CN101104866A - Technique for producing protein peptide and isoflavone extracted from bean sprout - Google Patents
Technique for producing protein peptide and isoflavone extracted from bean sprout Download PDFInfo
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- CN101104866A CN101104866A CNA2006100129588A CN200610012958A CN101104866A CN 101104866 A CN101104866 A CN 101104866A CN A2006100129588 A CNA2006100129588 A CN A2006100129588A CN 200610012958 A CN200610012958 A CN 200610012958A CN 101104866 A CN101104866 A CN 101104866A
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Abstract
The invention discloses a production process of extraction soybean peptide and isoflavone from bean sprout, comprising refining, filtering, acid precipitation, centrifugal separation for protein solution, purified water call back, decolor, desalination, call back, enzymatic hydrolysis, ion exchange, drying concentration to get soybean peptide; desalination, resin adsorption, alcohol washing, dealcoholization, drying concentration to centrifugal separated whey solution to get soybean isoflavone. The invention adopts bean sprout as raw material and percolation technique by ultrafiltration membrane, which can not only save energy and recycle waste water, but also can realize cleaner production with discharge of waste water reduced.
Description
Technical field
The present invention relates to a kind of soybean deep processing technology, specifically, relate to a kind of production technique of utilizing bean sprouts processing to extract protein peptide, isoflavones.
Background technology
Known from institute, soybean has high-quality soy-protein, can extract soybean protein peptide, isoflavones, oligose, soybean fibers, Soyasaponin etc. from soybean protein, and causes people's attention.And fields such as medicine, food and health care have been widely used in.For this reason, this technical elements has been carried out deep research and development.In this respect, relevant report is arranged also, as Chinese invention patent, its notification number is CN1687123A, disclosed a kind of " membrane process for extracting soybean protein peptide and technique of deep processing byproduct thereof ".This technology is: with low temperature soy meal, through the alkali lye lixiviate, press the alkali lye solid-liquid ratio, after bean dregs are isolated with whizzer in the immersion back, utilize the polysulfone membrane ultrafiltration, separating whey solution and protein peptide solution; Protein peptide solution is heated, sterilizes, protein solution is homogenized; Protein solution behind the homogeneous is added protease hydrolysis, utilizes the polysulfone membrane ultrafiltration again, concentrate, dry, promptly obtain soybean protein peptide; Utilize isolated whey solution to adsorb by macroporous resin again, adsorb the soybean isoflavones composition in the whey solution, by utilizing ethanolic soln macroporous resin is carried out wash-out, obtain soybean isoflavones solution, drying obtains soybean isoflavones; Utilize the whey solution of the resin absorption of the soybean isoflavones composition of deviating from again, through the selective adsorbing resin column of Daidzin is adsorbed, make the soybean saponin glucoside from solution, be adsorbed onto on the resin, by heating the Soyasaponin adsorption column is inhaled again and taken off, obtain Soyasaponin solution, after super-dry, obtain the Soyasaponin of finished product then; Soyasaponin that again will be through deviating from is by the whey solution of adsorption column, add in the hydrochloric acid and after, by anionite-exchange resin and Zeo-karb, obtain soybean oligosaccharide solution, through concentrate drying, obtain soybean oligosaccharide.
Above-mentioned embrane method is to produce soybean protein isolate and protein peptide, solves through the heavy method gelation of traditional alkali pickling, emulsifying property, oil absorbency, water-retentivity, solvability, foaminess and the relatively poor defective that can't satisfy the foodstuffs industry needs of froth stability.But in actual production, owing to adopt the dregs of beans raw material to make emulsion by processing.Wherein contain the profit composition in the emulsion, with discharge of wastewater, contain certain oily matter like this in waste water after separation and Extraction, when the waste water that is discharged meeting generation peculiar smell behind long-term aeration, and the polluted air environment does not meet environmental requirement.And administer this exhaust emission and need dispose sewage treatment equipment.The sewage treatment equipment price is rather stiff of this special use.Therefore above-mentioned technology for a manufacturing enterprise, is that sizable difficulty is arranged, thereby has also just restricted the application of this production technique in actual applications.
For this reason, this field presses for the production technique of the soybean protein peptide that a kind of non-environmental-pollution is provided.
Summary of the invention
The objective of the invention is by utilizing the soybeans soaking germination technology, to extract soybean protein peptide, isoflavones for addressing the above problem.
Another object of the present invention has provided the production technique of extracting soybean protein peptide, isoflavones and byproduct thereof.Finished the present invention on this basis.
Production technique of the present invention may further comprise the steps:
The pre-treatment of raw material: with soybeans soaking 3-6 days, temperature 24-28 ℃ to germinateing; Before the use
It is cleaned, but standby except that do not germinate beans and skin of beancurd.
Production technique:
1) bean sprouts material, water were carried out defibrination 80 orders than 1: 4 and filter, filter through the 100-120 order, filter residue is standby;
2) solution is added 20% edible hydrochloric acid or citric acid, phosphoric acid; Transfer PH=4.5, stir 30 rev/mins, precipitate 30 fens;
3) filtrate of above-mentioned heavy acid is carried out centrifugation, separating whey solution and albumen precipitation thing;
4) throw out is washed, separated, secondary water washing, temperature are 60 ℃, carry out secondary separation;
5) the albumen precipitation thing is added NaOH, accent PH=6～7, and be heated to 90 ℃, 10 minutes time was carried out the water purification readjustment;
6) with the above-mentioned activated carbon decolorizing that utilizes, reverse osmosis desalination, high-temperature short-time sterilization;
7) adjust back above-mentioned, adjust PH=6, between 5～7, concentration 18%-25%;
8), add NaOH and transfer PH=8,5 with 80 ℃ of above-mentioned heating;
9) with above-mentioned adding hydrolysis by novo, temperature is controlled at 65-70 ℃, transfers PH=7～8,6 hours time;
10) above-mentioned enzymolysis solution is entered ultra-fine filter and separate, adopt two film spares to handle, molecular weight cut-off is respectively 300-1000.300, returns the continuation hydrolysis greater than 1000;
11) be that the enzymolysis solution of 300-1 000 adds gac and carries out debitterize, decolouring with molecular weight;
12) carry out 135 high-temperature short-time sterilization after enzymolysis solution being entered the ion-exchanger desalination;
13) enzymolysis solution is carried out the vacuum concentration drying, promptly obtain the finished product soybean protein peptide;
14) the isolated whey solution of each step 3 heats 70 ℃, removes residual protein;
15) with the whey solution of purifying treatment, squeeze into desalination plant, remove 95% salt;
16), send into resin column absorption other component wherein with the whey solution behind the desalination;
17) above-mentioned ethanol with 20%-80% is carried out gradient elution, collect elutriant, carry out dealcoholysis, concentrate, be drying to obtain soybean isoflavones.
Also can make following product by production technique of the present invention:
1, with after above-mentioned steps 7 dryings, promptly obtains soybean protein isolate.
2, the whey solution of above-mentioned steps 16 adsorption treatment is carried out the reverse osmosis dehydration, solution sugar concentration is reached more than 12%, dialysis water can repeatedly utilize, and the dense back solution sugar that contracts is sent into vacuum-evaporation and can be concentrated and reach 30%; Enter ultra-filtration equipment and carry out membrane sepn, remove suspended substance and bacterium, purge drying promptly obtains soybean oligosaccharide;
3, with the filter residue of above-mentioned steps 1, water was carried 10 minutes in 1: 3, crossed 80 orders, and filter residue and drying promptly obtains soybean fibers.
The positively effect that the present invention had is:
1, the seed selection of soybean varieties
Because soybean varieties NSI value exists than big-difference, and is bigger with 2S, 7S, 11S and 15S sphaeroprotein proportional difference in the soy-protein.Be the productive rate and the function of raising soybean varieties, necessary seed selection 7S and the high soybean varieties of 11S content, therefore, soy material selects northeast granule soya bean as the main separation raw material.
2, novel soybean germination technology
Soybean germination is a kind of biological action, along with seed imbibition cell water content increases, enzymic activity strengthens, insoluble polymer nutritive substance is converted into the simple material of solubility.The one, germinateing raises the total NSI value of soybean seeds, and aminoacids content raises; The 2nd, metabolism of fat.In the soybean germination process, fat is resolved into glycerine and lipid acid by enzyme, and generates carbohydrate at last, and the 3rd, the increase of carbohydrate; The 4th, in the soybean germination process, the harmful anti-nutrition of part is because of fading away.
3, protease treatment technology
Adopted Sumizyme MP to carry out the hydrolysis of soybean protein in this technology, thereby the protein molecule peptide chain is shortened.Effectively obtain emulsifying property, retentiveness, whipability, solvability, dispersed better protein product.Utilize the hydrolyzate of Sumizyme MP control soybean protein, not only have higher water-solublely, mobile, and can provide the human body needed nutritive substance that grows, have diseases prevention, cure the disease and regulate the effect of human body physiological function.
4, be raw material because of having adopted beans material, utilize ultra-filtration membrane diafiltration technology, not only energy-conservationly make cycling utilization of wastewater, but also realized cleaner production, reduced the discharging of waste water to germinate.
Embodiment
Below in conjunction with specific embodiment, the present invention is described in further detail, and institute be should manage and reference and explanation usefulness only are used for to embodiment, be not to be used to limit the scope of the invention.
In the concrete enforcement of the present invention, earlier its raw material is carried out pre-treatment:
1, select the granule soya bean in the place of production, northeast, soak through washing only, controlled temperature is at 24-28 ℃ of trickle, and lucifuge was germinateed in 3-6 days;
2, above-mentioned germination beans are cleaned, but remove do not germinate beans and skin of beancurd.
Embodiment 1 (soybean protein isolate)
1, defibrination: with the ratio of germination beans material and water is 1: 4, carries out defibrination 80 orders by paste roller mill and is filtered into slurry;
2, filter: it is standby that above-mentioned slurry is crossed the elimination filter residue with 100～120 orders;
3, heavy acid: with 20% edible hydrochloric acid or citric acid, phosphoric acid: transfer PH=4.5, stir 30 rev/mins, precipitate 30 minutes;
4, centrifugation: with above-mentioned centrifuge dehydration, separating whey solution and the albumen precipitation thing of carrying out;
5, above-mentioned albumen precipitation thing is washed centrifugations with 60 ℃ of warm water, respectively carry out twice;
6, water purification readjustment: above-mentioned adding NaOH is transferred PH=6～7, and heat 90 ℃, 10 minutes;
7, decolouring, desalination: its albumen precipitation thing is decoloured reverse osmosis desalination by gac;
8, sterilising treatment: above-mentioned albumen precipitation thing through decolouring, desalting treatment is carried out 120 ℃ of high-temperature short-time sterilization;
9, readjustment: adjust PH=6,5～7, between 0, concentration 18%-25%;
10, drying:, promptly obtain soybean protein isolate by bedroom spray thing drying treatment.
Embodiment 2 (soybean protein peptide)
1, presses 80 ℃ of poly-9 readjustment post-heating of 1 step of embodiment, add NaOH and transfer PH=8;
2, with above-mentioned adding hydrolysis by novo, temperature is controlled at 65～70 ℃, transfers PH=7～8, and the time is 6 hours;
3, above-mentioned enzymolysis solution is entered ultra-fine filter and separate, adopt two film spares to handle, molecular weight cut-off is respectively: 300-1000,300; Return the continuation hydrolysis greater than 1000;
4, molecular weight cut-off is existed: the enzymolysis solution of 300-1000 adds gac and carries out debitterize, decolouring;
5, carry out 135 ℃ of ultra high temperature short time sterilizations after above-mentioned enzymolysis solution being entered the ion-exchanger desalination;
6, above-mentioned enzymolysis solution is carried out the vacuum concentration drying, promptly obtain the finished product soybean protein peptide.
Embodiment 3 (soybean oligosaccharide)
1, presses isolated whey solution in embodiment 1 step 4, be heated to 70 ℃, purify and remove residual protein;
2, desalination: with the soybean whey solution of above-mentioned purifying treatment, squeeze into desalination plant, remove 95% salt;
3, resin absorption: the soybean whey behind the desalination, enter resin absorption other component wherein, improve mouthfeel, the local flavor of sugar, and will hang down colour, simultaneously decolouring;
4, concentrate: utilize the reverse osmosis dehydration, solution sugar concentration is reached more than 12%.Dialysis water can repeatedly utilize, but after concentrating solution sugar is sent into vacuum-evaporation simmer down to 30%;
5, purify: the solution sugar with 30% enters ultra-filtration equipment and carries out membrane sepn, removes suspended substance and bacterium, is drying to obtain soybean oligosaccharide.
Embodiment 4 (soybean isoflavones)
Press embodiment 3 step poly-3 and handle through the resin absorption thing, carry out gradient elution with the ethanol of 20%-80%, the collection elutriant carries out dealcoholysis concentrating under reduced pressure drying, promptly obtains soybean isoflavones;
Embodiment 5 (soybean fibers)
Press 1 step poly-2 of embodiment with filter residue, press 1: 3 flooding of material-water ratio and filtered 80 orders in 10 minutes, filter residue and drying promptly obtains soybean fibers.
Claims (1)
1. the production technique of soybean protein peptide, isoflavones is extracted in a bean sprouts, may further comprise the steps:
1) defibrination: with the germination beans, pressed ratio of water to material 4: 1, carry out defibrination 80 orders and filter;
2) filter: the 100-120 order filters, and filter residue is standby;
3) acid is heavy: with 20% edible hydrochloric acid/or citric acid, phosphoric acid, accent PH=4.5 stirs 30 rev/mins, precipitates 30 minutes;
4) centrifugation: centrifuge dehydration, dehydration separating whey solution and albumen precipitation thing;
5) water purification readjustment: above-mentioned albumen precipitation thing is added NaOH, transfer PH=6-7, and heat 90 ℃, 10 minutes;
6) decolouring, desalination: use activated carbon decolorizing, reverse osmosis desalination;
7) sterilization: 135 ℃ of high-temperature short-time sterilization,
8) readjustment: transfer PH=6,5～7, between 0, concentration 18%-25%;
9) with 80 ℃ of above-mentioned readjustment post-heating, add NaOH, transfer PH=8,5, add hydrolysis by novo, temperature is transferred PH=7～8 at 65～70 ℃, 6 hours time, send into ultra-fine filter and separate, molecular weight cut-off is respectively: 300-1000,300 enters the ion-exchanger desalination, through 135 ℃ of high-temperature sterilizations, concentrate drying promptly obtains soybean protein peptide;
10) with 70 ℃ of isolated whey solution heating of above-mentioned steps poly-4, remove residual protein; Desalination to 95% after the purifying treatment; Enter resin column absorption other component wherein, and add 20%-80% ethanol and carry out gradient elution, collect elutriant carry out dealcoholysis concentrate, dry, promptly obtain soybean isoflavones.
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Cited By (11)
| Publication number | Priority date | Publication date | Assignee | Title |
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| CN102000138A (en) * | 2010-10-28 | 2011-04-06 | 中国科学院新疆理化技术研究所 | Method for purifying effective ingredients of cicer ariethnum L. bean sprouts |
| CN102010460A (en) * | 2010-09-27 | 2011-04-13 | 厦门盛洲植物油有限公司 | Tea seed polypeptide and preparation method thereof |
| CN102429309A (en) * | 2011-11-18 | 2012-05-02 | 上海交通大学 | Micro-capsulated germinated soybean peptide crude extract effervescent tablet and preparation method thereof |
| CN102475191A (en) * | 2010-11-26 | 2012-05-30 | 台菌生技股份有限公司 | Plant protein product and preparation method thereof |
| CN103146788A (en) * | 2013-02-28 | 2013-06-12 | 珠海博康药业有限公司 | Enzymic-method based preparation method of soybean peptide |
| CN103392904A (en) * | 2013-08-02 | 2013-11-20 | 江苏丘陵地区镇江农业科学研究所 | Method used for extracting black soya bean polypeptide by combining wet ball milling with enzyme method |
| CN105586178A (en) * | 2016-01-25 | 2016-05-18 | 王家高 | Non-toxic detergent and preparation method thereof |
| CN105779550A (en) * | 2016-04-11 | 2016-07-20 | 浙江省农业科学院 | Method for preparing bean sprouting functional peptide |
| CN107156307A (en) * | 2017-07-11 | 2017-09-15 | 济源市能力源生物饮品有限公司 | A kind of soy-bean peptide and its preparation technology |
| CN108813615A (en) * | 2018-07-05 | 2018-11-16 | 中国农业科学院农产品加工研究所 | The preparation method of mung bean extracting solution with antioxidant activity |
| CN114468106A (en) * | 2022-03-11 | 2022-05-13 | 岭南师范学院 | Protease M-assisted isoflavone peptide-barley-folium Rumicis gel soft candy and preparation method thereof |
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Cited By (15)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102010460A (en) * | 2010-09-27 | 2011-04-13 | 厦门盛洲植物油有限公司 | Tea seed polypeptide and preparation method thereof |
| CN102010460B (en) * | 2010-09-27 | 2012-08-22 | 厦门盛洲植物油有限公司 | Tea seed polypeptide and preparation method thereof |
| CN102000138A (en) * | 2010-10-28 | 2011-04-06 | 中国科学院新疆理化技术研究所 | Method for purifying effective ingredients of cicer ariethnum L. bean sprouts |
| CN102000138B (en) * | 2010-10-28 | 2012-04-18 | 中国科学院新疆理化技术研究所 | Method for purifying effective ingredients of cicer ariethnum L. bean sprouts |
| CN102475191A (en) * | 2010-11-26 | 2012-05-30 | 台菌生技股份有限公司 | Plant protein product and preparation method thereof |
| CN102429309A (en) * | 2011-11-18 | 2012-05-02 | 上海交通大学 | Micro-capsulated germinated soybean peptide crude extract effervescent tablet and preparation method thereof |
| CN103146788A (en) * | 2013-02-28 | 2013-06-12 | 珠海博康药业有限公司 | Enzymic-method based preparation method of soybean peptide |
| CN103392904A (en) * | 2013-08-02 | 2013-11-20 | 江苏丘陵地区镇江农业科学研究所 | Method used for extracting black soya bean polypeptide by combining wet ball milling with enzyme method |
| CN105586178A (en) * | 2016-01-25 | 2016-05-18 | 王家高 | Non-toxic detergent and preparation method thereof |
| CN105779550A (en) * | 2016-04-11 | 2016-07-20 | 浙江省农业科学院 | Method for preparing bean sprouting functional peptide |
| CN107156307A (en) * | 2017-07-11 | 2017-09-15 | 济源市能力源生物饮品有限公司 | A kind of soy-bean peptide and its preparation technology |
| CN108813615A (en) * | 2018-07-05 | 2018-11-16 | 中国农业科学院农产品加工研究所 | The preparation method of mung bean extracting solution with antioxidant activity |
| CN108813615B (en) * | 2018-07-05 | 2022-06-07 | 中国农业科学院农产品加工研究所 | Preparation method of mung bean extract with antioxidant activity |
| CN114468106A (en) * | 2022-03-11 | 2022-05-13 | 岭南师范学院 | Protease M-assisted isoflavone peptide-barley-folium Rumicis gel soft candy and preparation method thereof |
| CN114468106B (en) * | 2022-03-11 | 2024-01-16 | 岭南师范学院 | Protease M-assisted iso Huang Tongtai-barley grass gel soft sweet and preparation method thereof |
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