CN101095690A - Rhodiola sacra extractive, medicinal composition containing the same, method for preparing the same and the use thereof - Google Patents
Rhodiola sacra extractive, medicinal composition containing the same, method for preparing the same and the use thereof Download PDFInfo
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Abstract
The invention provides a gadol extract. The extract comprises rosavins, with the weight proportion being 3.0-10.0%, and the rosavins comprises rosavin, rosarin and rosin. The invention also relates to the method for preparing said extract and utilization. The invention is characterized by distinctive effect and strong controllable.
Description
Technical field
The present invention relates to a kind of Radix Rhodiolae extract, belong to drug world.
Background technology
The Radix Rhodiolae nature and flavor are sweet, and are puckery, cold, are used for promoting blood circulation and hemostasis, clearing away lung-heat to relieve cough.The cough of treatment pneumonia, the spitting of blood hemoptysis, the women leucorrhea, traumatic injury, burn are controlled in external.Recent study shows that Radix Rhodiolae has the effect of anti-hypoxia, cold resistance, resisting fatigue, resisting microwave radiation and excited brain and spinal cord.Can improve the work efficiency of muscle power and mental work, delay body aging, prevent and treat functions such as infirmities of age.Crassulaceae Rhodida plant (Rhodiola L.) is perennial herb or semishrub plant, and global rhodiola has kind more than 90, and China has kind more than 80 approximately.
At present, about rhodiola rosea formulated product, report morely, provide a kind of safe as JP2001240552, the alpha-amylase inhibitor of pure natural is comprising the extract of Radix Rhodiolae and other plant.Difference: the one, this patent product contains other extract except that Radix Rhodiolae extract, and active component is indeterminate; The 2nd, the Radix Rhodiolae raw material variety difference that relates in this patented product.The JP2000128795 aldose reductase inhibitor is used for the diabetes peripheral nerve injury such as amyotrophy, and product contains the powder of Rhodida plant or extract as effective ingredient.Preferred Rhodiolasacra, this patent provides taking dose, but this product uses is to comprise the powder of Herb of Radix Rhodiolae or alcohol, water extract, and purposes is the diabetes peripheral nerve injury.The JP2000128793 alpha-glucosidase inhibitor, product contains the powder of Rhodida plant or extract as effective ingredient, preferred Rhodiola sacra, patent text provides taking dose, but this patent product uses is to comprise the powder of Herb of Radix Rhodiolae or alcohol, water extract.
Have at hypoglycemic report at present: Zhao Wen, imperial court is glad, and Jiang Dongsheng, Rhodiola rosea L. extracting solution be to the repair of diabetic mice pancreatic function, Chinese comparative medicine magazine, 2003,13 (5): 309; The serum insulin levels of report Rhodiola rosea L. extracting solution (alcohol, water are slightly carried) the alloxan type diabetic mice that can obviously raise, and can obviously improve the damage that alloxan is caused the mouse islets tissue, prompting Rhodiola rosea L. extracting solution has certain repair to the diabetic mice pancreatic function.Zhao Wen, imperial court is glad, and Jiang Dongsheng etc., Rhodiola rosea L. are to the regulating action of alloxan diabetes mouse blood sugar, health research, the effect of 28:362-263 Rhodiola rosea L. extracting solution, alcohol, water crude extract.Described result of the test shows, Radix Rhodiolae extract can reduce the fasting blood sugar of alloxan diabetes mice at dosage during for 2500mg/kg.BW more significantly, and strengthen its carbohydrate tolerance ability, and when dosage is 5000mg/kg.BW, it falls the DeGrain of fasting glucose, but the carbohydrate tolerance ability of animal pattern is obviously strengthened.Simultaneously, Radix Rhodiolae extracting solution can make hepatic glycogen of alloxan diabetes mice and serum calcium levels obviously increase in the 1250-5000mg/kg.BW dosage range, and the decrease to some degree of serum urea fluorine content.The author think Radix Rhodiolae extracting solution blood sugar reducing function may to be rich in chromium relevant with it.Cheng Xiujuan, Di Lin, Wu Yan etc., Radix Rhodiolae polysaccharide for reducing blood sugar effect---the comparison of different way of administration, 1996 the 21st volumes of CHINA JOURNAL OF CHINESE MATERIA MEDICA o. 11th 686-691, Rhodiola sachalinensis polyoses extract has hypoglycemic activity, and the Radix Rhodiolae that this report is used is Rhodiola sachalinensis.
But because Radix Rhodiolae kind source is complicated, and ingredient complexity, effective substance can not be clear and definite, clinical use is difficult to effectively control usage and the consumption of medicine at different indications, stability, controllability is all relatively poor, also do not have the report of related pins to the corresponding index composition of its different indications at present.
Summary of the invention
Technical problem to be solved by this invention has provided a kind of stable, controlled Radix Rhodiolae extract, and the technical problem of another solution of the present invention has provided this preparation method of extract and purposes.
The invention provides a kind of Radix Rhodiolae extract, it is to be the extract that raw material is prepared from through extraction by Radix Rhodiolae, the weight percentage that this extract contains the plain glycoside rosavins of phenylpropyl alcohol is 3.0-10.0%, and wherein the plain glycoside rosavins of phenylpropyl alcohol is made up of cinnamyl alcohol glycosides rosavin, rosarin, rosin.Wherein rhodiola rosea is called rose-red Herba hylotelephii erythrosticti or Rhodiola rosea L. in China, the plain glycoside rosavins of its composition phenylpropyl alcohol (comprises rosavin, rosarin, rosin) be the main feature that rhodiola rosea is different from other kind Radix Rhodiolae, in rhodiola plant, rosavins only is present in the rhodiola rosea rhizome.
Described Radix Rhodiolae derives from Crassulaceae rhodiola rhodiola rosea rhizome.
Further, the weight percentage that contains rosavins in the Radix Rhodiolae extract of the present invention is 3.0-5.0%.
Wherein, also contain rhodioside salidroside in this extract, wherein the weight percentage of rhodioside salidroside is 0.5-1.0%.
Further, the weight percentage that contains rosavins in the extract is 3.0-5.0%, and the weight percentage of rhodioside salidroside is 0.8-1.0%.
The present invention also provides the preparation method of this Radix Rhodiolae extract, and it comprises the steps:
A, to get Radix Rhodiolae rhodiola rosea be raw material, water or ethanol, or aqueous alcohols is extracted, is concentrated;
B, get the described concentrated solution of a step, last macroporous adsorptive resins washes non-adsorbable impurity, and reuse 50-70% ethanol is washed, eluent, with the eluent drying.
The invention provides this Radix Rhodiolae extract and have purposes in the medicine that suppresses alpha-glucosidase activity in preparation.
The present invention also provides this Radix Rhodiolae extract to have purposes in the hypoglycemic medicine in preparation.
Wherein, described medicine is to have the medicine that reduces the post-prandial glycemia effect.
With above-mentioned said Radix Rhodiolae extract of the present invention, after pharmaceutically acceptable auxiliary adding ingredient mixes,, can prepare the medicine of a-glucosidase inhibitor class by corresponding conventional medicine formulation method.For example, after in oral formulations, can receivedly mixing as auxiliary adding ingredient commonly used such as disintegrating agent, excipient, lubricant, binding agent, filler, operational approach routinely and process can be made the medicine of tablet, pill, capsule or solid orally ingestible forms such as multiple corresponding slow releasing agent, controlled release agent.
Radix Rhodiolae extract drug effect of the present invention is obvious, and controllability is strong, provides a kind of new selection for clinical.
Obviously, according to foregoing of the present invention,,, can also make modification, replacement or the change of other various ways not breaking away under the above-mentioned basic fundamental thought of the present invention prerequisite according to the ordinary skill and the customary means of this area.
The specific embodiment of form is described in further detail foregoing of the present invention again by the following examples.But this should be interpreted as that the scope of the above-mentioned theme of the present invention only limits to following example, all technology that realizes based on foregoing of the present invention all belong to scope of the present invention.
Description of drawings
Fig. 1 Radix Rhodiolae extract of the present invention is to a-amylase inhibitory action
Fig. 2 Radix Rhodiolae extract of the present invention is to the maltase inhibitory action
Fig. 3 Radix Rhodiolae extract of the present invention is to the inhibitory action of saccharase
The specific embodiment
The preparation of embodiment 1 Radix Rhodiolae extract of the present invention:
200g Radix Rhodiolae rhodiola rosea rhizome, the alcohol reflux of 2000ml70% 1 hour, filter, the ethanol extraction of reuse 1000ml70% 1 hour, filter, filtering residue is with the ethanol of 1000ml70% reflux, extract, 1 hour again, merge extractive liquid, concentrates D101 resin on the concentrated solution, elder generation's water eluting, the ethanol elution of reuse 50%, the concentrate drying ethanol elution gets the 13.5g extract, it is 3.51% that HPLC detects the percentage composition that contains rosavins, and the percentage composition of salidroside is 0.93%.
The preparation of embodiment 2 Radix Rhodiolae extracts of the present invention:
200g Radix Rhodiolae rhodiola rosea rhizome, the alcohol reflux of 2000ml70% 1 hour, filter, the ethanol extraction of reuse 1000ml70% 1 hour, filter, filtering residue is with the ethanol of 1000ml70% reflux, extract, 1 hour again, merge extractive liquid, concentrates D101 resin on the concentrated solution, elder generation's water eluting, the ethanol elution of reuse 70%, the concentrate drying ethanol elution gets the 13.6g extract, it is 4.18% that HPLC detects the percentage composition that contains rosavins, and the percentage composition of salidroside is 0.76%.
The preparation of embodiment 3 Radix Rhodiolae extracts of the present invention:
200g Radix Rhodiolae rhodiola rosea rhizome, the alcohol reflux of 2000ml70% 1 hour, filter, the ethanol extraction of reuse 1000ml70% 1 hour, filter, filtering residue is with the ethanol of 1000ml70% reflux, extract, 1 hour again, and merge extractive liquid, concentrates, D101 resin on the concentrated solution, elder generation's water eluting, the ethanol elution of reuse 30%, reuse 70% ethanol elution, concentrate drying 70% ethanol elution, get the 10.9g extract, it is 8.04% that HPLC detects the percentage composition that contains rosavins, and the percentage composition of salidroside is 0.57%.
Under the constant situation of above-mentioned basic technology, carry out the choose reasonable of conditional parameter, just can obtain the Radix Rhodiolae extract of the present invention of different size.
The preparation of embodiment 4 Radix Rhodiolae crude extracts:
200g Radix Rhodiolae rhizome, the alcohol reflux of 2000ml70% 1 hour filters the ethanol extraction of reuse 1000ml70% 1 hour, filter, filtering residue is with the ethanol of 1000ml70% reflux, extract, 1 hour again, merge extractive liquid,, concentrate drying, get 45g, HPLC detects, and the percentage composition that contains rosavins is 1.25%, and the percentage composition of salidroside is 1.35%.
The preparation of embodiment 5 medicines of the present invention
With above extract (extract of embodiment 1-3 preparation) powder, granulate with one-step-granulating method, be wetting agent with water, inlet temperature 60-80 ℃, leaving air temp 40-60 ℃, get granule, packing gets the granule product.
With above extract (embodiment 1-3 preparation extract) powder or granule, incapsulate the capsule product.
After being processed into granule by above method, add an amount of wetting agent and disintegrating agent again, tabletting, every 0.45-0.55g, the high-efficiency coating machine coating, packing gets the tablet product.
Embodiment 6 Radix Rhodiolae extract detection methods of the present invention
The detection method of rosavins:
One, chromatographic condition:
Inertsil ODS 150×4.6mm 3.5um
Mobile phase: the water of mobile phase 0.1% phosphoric acid acetonitrile solution in the time of 0-22 minute becomes 70% by 90%.
Column temperature: room temperature
Flow velocity: 1.0ml/min
Detect wavelength: 254nm
Sample size: 10ul
Sensitivity: 0.01AUFS
Two, formulations prepared from solutions:
1, the preparation of standard solution
Precision takes by weighing rosavin, rosarin, the rosin reference substance that is dried to constant weight through phosphorus pentoxide, respectively adds methanol and makes the solution that every 1ml contains 0.15mg respectively, in contrast product solution.
Draw contrast liquid 2,4,6,8,10ml are mixed with five kinds of variable concentrations with methanol in the 50ml volumetric flask reference substance solution more respectively.
2, the preparation of need testing solution
The fixed two duplicate samples 0.6g of accurate respectively title are in two 100ml volumetric flasks.The analysis methanol that adds about 70ml, ultrasonic dissolution in ultrasound wave is put and is chilled to room temperature, is settled to scale, promptly gets testing sample with the 0.45um filtering with microporous membrane then.
Three, sample determination:
Under above-mentioned chromatographic condition, treat instrument stabilizer, after baseline was steady, sample introduction was measured; Content calculates by external standard method.
The rhodioside detection method
One, chromatographic condition:
Inertsil ODS 150×4.6mm 3.5um
Mobile phase: water: methanol: phosphoric acid (volume ratio 80: 20: 0.2)
Column temperature: room temperature
Flow velocity: 1.0ml/min
Detect wavelength: 225nm
Sample size: 10ul
Sensitivity: 0.01AUFS
Two, the preparation of solution:
1, the preparation of standard solution
Precision takes by weighing the rhodioside reference substance that is dried to constant weight through phosphorus pentoxide, respectively adds methanol and makes the solution that every 1ml contains 0.15mg respectively, in contrast product solution.
Draw contrast liquid 2,4,6,8,10ml are mixed with five kinds of variable concentrations with methanol in the 50ml volumetric flask reference substance solution more respectively.
2, the preparation of need testing solution
The fixed two duplicate samples 0.6g of accurate respectively title are in two 100ml volumetric flasks.The analysis methanol that adds about 70ml, ultrasonic dissolution in ultrasound wave is put and is chilled to room temperature, is settled to scale, promptly gets testing sample with the 0.45um filtering with microporous membrane then.
3, sample determination:
Under above-mentioned chromatographic condition, treat instrument stabilizer, after baseline was steady, sample introduction was measured; Content calculates by external standard method.
Below prove beneficial effect of the present invention by pharmacodynamics test.
Test of the inhibition determination of activity of example 1 Radix Rhodiolae extract of the present invention to the a-glucosidase:
Sample is respectively: 1, embodiment 1 prepared product; 2, embodiment 2 prepared products;
3, embodiment 3 prepared products; 4, embodiment 4 prepared products.
Experimental program:
When 1) being substrate with starch, Radix Rhodiolae extract is to the inhibition determination of activity of a-glucosidase:
The Radix Rhodiolae extract of embodiment 1-4 preparation is dissolved in normal saline respectively, being mixed with concentration is 1mg/ml, 0.5mg/ml, 025mg/ml, 0.125mg/ml, 0.0625mg/ml Radix Rhodiolae extract solution, again respectively with these three kinds of solution 20 microlitres and 2 microlitre a-amylase (0.1U/ul, available from SIGMA company) mix, 37 ℃ of incubations 10 minutes, add 1% starch solution, incubated at room 3～5 minutes, use the iodometric titration development process, under the 630nm wavelength, measure the OD value, calculate a-glucosidase activity suppression ratio by the consumption of starch amount; Set blank, negative control, positive control (acarbose (manufacturer: Beijing Bayer HealthCare Co)), suppression ratio=(sample-feminine gender/blank-feminine gender) * 100%
When 2) being substrate with maltose, Radix Rhodiolae extract is to the inhibition determination of activity of a-glucosidase:
The Radix Rhodiolae extract of embodiment 1-4 preparation is dissolved in normal saline respectively, be mixed with 1mg/ml, 0.1mg/ml, 1 μ g/ml, 0.1 μ g/ml solution, again respectively with these three kinds of solution 20 microlitres and 2 microlitre a-glucosidase (0.02U/ul, purchase SIGMA company) mix, 37 ℃ of incubations 10 minutes, add maltose then, 37 ℃ were continued incubation 10 minutes; Adopt glucose oxidase method, under the 490nm wavelength, measure the OD value,, calculate a-glucosidase activity suppression ratio with the growing amount of glucose; Set blank, negative control, positive control (acarbose).Enzymatic activity suppression ratio=(feminine gender-sample)/feminine gender-blank) * 100%.
When 3) being substrate with sucrose, Radix Rhodiolae extract is to the inhibition determination of activity of a-glucosidase:
The Radix Rhodiolae extract of embodiment 1-4 preparation is dissolved in normal saline respectively, be mixed with 1mg/ml, 0.1mg/ml, 0.01mg/ml, 0.001mg/ml solution, again respectively with these three kinds of solution 20 microlitres and 2 microlitre invertase (0.106U/ul, purchase SIGMA company) mix, 37 ℃ of incubations 10 minutes, add sucrose then, 37 ℃ were continued incubation 10 minutes; Adopt glucose oxidase method, under 490 wavelength, measure the OD value,, calculate a-glucosidase activity suppression ratio with the growing amount of glucose; Set blank simultaneously, negative control, positive control (acarbose).Enzymatic activity suppression ratio=(feminine gender-sample)/(feminine gender-blank) * 100%.
Fig. 1,2, the 3rd, Radix Rhodiolae extract is to the measurement result of the depression effect of a-glucosidase activity.Ordinate is represented the enzymatic activity suppression ratio, and abscissa is represented Radix Rhodiolae extract concentration.Compared among the figure with maltose, sucrose, when starch is substrate, Radix Rhodiolae extract is to the inhibition activity of a-glucosidase.
Fig. 1 result shows that Radix Rhodiolae extract is external to have significant inhibitory effect to a-amylase, and is wherein the strongest to the amylase inhibitory action with No. 2, secondly is No. 1, No. 3, No. 4.
Fig. 2 result shows that Radix Rhodiolae extract is external to have significant inhibitory effect to maltase.The extract inhibitory action of different content is suitable, does not have evident difference.
Fig. 3 result shows that Radix Rhodiolae extract is external to have significant inhibitory effect to saccharase.The extract inhibitory action of different content is suitable, does not have evident difference.
Conclusion: above experimental result shows that Radix Rhodiolae extract of the present invention is external to have remarkable inhibitory action to the a-glucosidase.And the extract of different content is directed to the different mechanism of action and has produced different inhibitory action.Particularly Radix Rhodiolae extract is external has significant inhibitory effect to a-amylase, wherein the strongest to the amylase inhibitory action with No. 2, next is No. 1, No. 3, No. 4, and promptly used Radix Rhodiolae extract of the present invention is better than the Radix Rhodiolae crude extract to the diastatic inhibitory action of a-.
Experiment (alloxan is induced the carbohydrate tolerance test of diabetic mice starch-bearing) in the test example 2 medicine bodies of the present invention
Get body weight 22-26 gram Kunming mouse, 200 milligrams of/kilogram alloxan of lumbar injection, measure blood glucose after 72 hours, choose the mice of blood glucose value more than 13mmol/L, by 10 every group, male and female half and half are divided into 6 groups, it is diabetic model group, Radix Rhodiolae crude extract group (embodiment 4 provides the preparation method of crude extract), Radix Rhodiolae extract (embodiment 1 preparation) high dose group, middle dosage group, low dose group, administration 10 minutes (model group is irritated the stomach normal saline), each group is all irritated stomach starch 5 gram/kilograms, in 0min, 30min, 60min gets vena orbitalis posterior clump blood behind the 120min, in 96 orifice plates, measure blood sugar content with glucose oxidase method, the results are shown in Table 1:
Table 1 pair alloxan causes the influence (x ± s) of diabetic mice carbohydrate tolerance
| Group | Dosage (g/kg) | Blood glucose value (mmol/L) | |||
| 0min | 30min | 60min | 120min | ||
| Model group crude extract group Radix Rhodiolae extract | 1.2 0.1 0.2 0.4 | 28.0±5.56 29.0±5.41 29.1±5.42 28.1±5.20 28.1±5.33 | 34.41±6.20 28.40±4.16 27.72±5.09 * 26.70±4.34 ** 24.81±4.30 * | 35.36±5.57 34.40±4.53 35.47±4.48 34.98±3.59 30.30±4.47 * | 33.0±487 30.7±4.07 33.9±3.71 32.8±3.62 31.5±3.76 |
Compare with model group,
*P＜0.05,
*P＜0.01.
Interpretation of result: from table as can be seen, after diabetic mice gave starch, blood glucose value obviously raise.The blood glucose value that Radix Rhodiolae extract can suppress diabetic mice raises.The blood glucose value of crude extract group has downward trend, but compares with matched group, does not have the significance meaning.
By the foregoing description, Radix Rhodiolae extract of the present invention is external that the a-glucosidase is had remarkable inhibitory action through experiment in vitro proof.Zoopery shows by the starch-bearing carbohydrate tolerance test, after giving dosage and being 100-400mg/kg.bw Radix Rhodiolae extract of the present invention, blood sugar increasing behind the remarkable reduction diabetic mice starch-bearing, rising to the control post-prandial glycemia is very effective, can be used for the particularly treatment of type ii diabetes and diabetic complication of I type.
Above-mentioned pharmacodynamics test proves that rhodiola plant rhodiola rosea of the present invention carries out quality control with rosavins.Evidence is 3.0-10.0% at the weight percentage of rosavins, steady quality, especially best results when the weight percentage of rosavins is 3.0-5.0%.Simultaneously rhodioside salidroside in the extract is carried out quality control, prove by experiment, the all non-single dose-effect relationship of rosavins and rhodioside salidroside in the medicine of the present invention, and (weight percentage of rosavins was 3.0-5.0% when two constituents reached ratio range, the weight percentage of salidroside is 0.8-1.0%), drug effect the best and controllability are good, provide a kind of stable, effective, controlled medicament selection for clinical.
Claims (10)
1, a kind of Radix Rhodiolae extract, it is characterized in that: it is to be the extract that raw material is prepared from through extraction by Radix Rhodiolae rhodiola rosea, the weight percentage that this extract contains the plain glycoside rosavins of phenylpropyl alcohol is 3.0-10.0%, and wherein the plain glycoside rosavins of phenylpropyl alcohol is made up of cinnamyl alcohol glycosides rosavin, rosarin, rosin.
2, Radix Rhodiolae extract according to claim 1 is characterized in that: the weight percentage that it contains rosavins is 3.0-5.0%.
3, Radix Rhodiolae extract according to claim 2 is characterized in that: also contain rhodioside salidroside in the extract, wherein the weight percentage of rhodioside salidroside is 0.5-1.0%.
4, Radix Rhodiolae extract according to claim 3 is characterized in that: the weight percentage that contains rosavins in the extract is 3.0-5.0%; The weight percentage of salidroside is 0.8-1.0%.
5, a kind of each described method of gadol extract of preparation claim 1-4, it comprises the steps:
A, get Radix Rhodiolae rhodiola rosea raw material, water or ethanol, or aqueous alcohols is extracted, is concentrated.
B, get the described concentrated solution of a step, last macroporous adsorptive resins washes non-adsorbable impurity, and reuse 50-70% ethanol is washed, eluent, with the eluent drying.
6, each described Radix Rhodiolae extract of claim 1-4 has purposes in the medicine that suppresses alpha-glucosidase activity in preparation.
7, each described Radix Rhodiolae extract of claim 1-4 has purposes in the hypoglycemic medicine in preparation.
8, purposes according to claim 7 is characterized in that: described medicine is to have the medicine that reduces the post-prandial glycemia effect.
9, a kind of pharmaceutical composition, it is to be active component by each described Radix Rhodiolae extract of claim 1-4, adds the medicament that acceptable accessories or complementary composition are prepared from.
10, pharmaceutical composition according to claim 9 is characterized in that: described medicament is an oral formulations.
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| CN102391316A (en) * | 2011-10-11 | 2012-03-28 | 泸州医学院 | Disaccharide compound and separation and purification method thereof |
| CN103131730A (en) * | 2011-11-30 | 2013-06-05 | 韩国食品研究院 | Method for manufacturing rhodiola fermentation product by using adsorbent |
| CN104306427A (en) * | 2014-11-06 | 2015-01-28 | 广州神农生物技术有限公司 | Rhodiola rosea extract and application thereof |
| CN104447901A (en) * | 2014-11-13 | 2015-03-25 | 安徽尚善生物科技股份有限公司 | Method for preparing rosavin from rose rhodiola rosea |
| CN104447900A (en) * | 2014-10-23 | 2015-03-25 | 石任兵 | Preparation activity and application of novel compound as well as quality control |
| CN107312047A (en) * | 2017-08-06 | 2017-11-03 | 中国科学院兰州化学物理研究所 | A kind of method that separated in synchronization from rhodiola root prepares rhodioside and network plug dimension |
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| CN110269876A (en) * | 2019-07-23 | 2019-09-24 | 中国科学院西北高原生物研究所 | Application of the Rhodiola crenulata extract in the food, drug or health care product that preparation inhibits amylase and glucosidase activity |
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| CN102391316B (en) * | 2011-10-11 | 2013-11-13 | 泸州医学院 | Disaccharide compound and separation and purification method thereof |
| CN102391316A (en) * | 2011-10-11 | 2012-03-28 | 泸州医学院 | Disaccharide compound and separation and purification method thereof |
| CN103131730A (en) * | 2011-11-30 | 2013-06-05 | 韩国食品研究院 | Method for manufacturing rhodiola fermentation product by using adsorbent |
| CN103131730B (en) * | 2011-11-30 | 2014-09-10 | 韩国食品研究院 | Method for manufacturing rhodiola fermentation product by using adsorbent |
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| CN104306427A (en) * | 2014-11-06 | 2015-01-28 | 广州神农生物技术有限公司 | Rhodiola rosea extract and application thereof |
| CN104447901A (en) * | 2014-11-13 | 2015-03-25 | 安徽尚善生物科技股份有限公司 | Method for preparing rosavin from rose rhodiola rosea |
| CN107312047B (en) * | 2017-08-06 | 2020-05-01 | 中国科学院兰州化学物理研究所 | Method for synchronously separating and preparing salidroside and rosavin from rhodiola rosea |
| CN107312047A (en) * | 2017-08-06 | 2017-11-03 | 中国科学院兰州化学物理研究所 | A kind of method that separated in synchronization from rhodiola root prepares rhodioside and network plug dimension |
| CN109021035A (en) * | 2018-09-18 | 2018-12-18 | 云南中烟工业有限责任公司 | A kind of phenyl acetanides, preparation method and use |
| CN109021035B (en) * | 2018-09-18 | 2021-04-06 | 云南中烟工业有限责任公司 | Phenylacetamide compound, preparation method and application thereof |
| CN110269876A (en) * | 2019-07-23 | 2019-09-24 | 中国科学院西北高原生物研究所 | Application of the Rhodiola crenulata extract in the food, drug or health care product that preparation inhibits amylase and glucosidase activity |
| US20230201286A1 (en) * | 2019-10-30 | 2023-06-29 | Plant Synergy Inc. | High yield extraction method for and products of rhodiola plants |
| US11793848B2 (en) * | 2019-10-30 | 2023-10-24 | Plant Synergy Inc. | High yield extraction method for and products of Rhodiola plants |
| CN110981924A (en) * | 2019-12-26 | 2020-04-10 | 天津市尖峰天然产物研究开发有限公司 | Method for preparing Luoshen from rhodiola rosea |
| CN112544634A (en) * | 2020-12-07 | 2021-03-26 | 吉林农业大学 | Rhodiola sachalinensis extract and application thereof |
| CN112544634B (en) * | 2020-12-07 | 2022-01-28 | 吉林农业大学 | Rhodiola sachalinensis extract and application thereof |
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