CN101089610A - Portable biochemical analyser based on surface plasma resonance - Google Patents

Abstract

A portable type of biochemical analysis device based on surface plasma resonance consists of host, microcomputer set in host, liquid crystal display on said computer and biological transducer. It is featured as setting infrared LED, polarization plate, linear-array CCD array detector, signal transmission cable and biological chip in biological transducer; arranging Au film on surface of glass base plate on biological chip and fixing biological sensitive film on Au film.

Description

A kind of portable biochemical analyser based on surface plasma resonance

Technical field

The present invention relates to the portable biochemical analyser of pick-up unit of a kind of CCD of the linear array based on surface plasma wave sensing.

Background technology

The detection modulator approach of existing surface plasma resonance (SPR) has following four kinds usually: (1) angular modulation: with the light incident of fixing wavelength X, change the incident angle θ of incident light wave, under a certain tested refractive index, according to equation $\sqrt{{\mathrm{\ϵ}}_{\mathrm{pr}}}\frac{\mathrm{\ω}}{c}\sin \mathrm{\θ}=\sqrt{\frac{{\widetilde{\mathrm{\ϵ}}}_1{\widetilde{\mathrm{\ϵ}}}_2}{{\widetilde{\mathrm{\ϵ}}}_1+{\widetilde{\mathrm{\ϵ}}}_2}}\left(\frac{\mathrm{\ω}}{c}\right),$ Always can find an incident angle θ, make it to satisfy resonant condition, produce surface plasma resonance.When the refractive index of sample changes, rescan incident angle, seek the resonance incident angle, set up the relation between resonance incident angle and the sample refractive index.The detection resolution of angular modulation method can reach 1 * 10 ^-7Deg RIU ^-1(RIU is a refractive index unit).(2) wavelength-modulated:, change the wavelength X of incident light wave with fixing incident angle θ incident.When refractive index one timing of sample, the scanning lambda1-wavelength makes it to satisfy resonant condition, produces surface plasma resonance, sets up the relation between resonance lambda1-wavelength and the sample refractive index.The detection resolution and the angular modulation of wavelength-modulated are basic identical, relatively are suitable for waveguide or optical fiber surface plasmon detection scheme.(3) intensity modulated: with fixing incident angle θ, fixing incident wavelength λ incident, measure the light intensity that produces when variations in refractive index and change, set up the relation between transmission light intensity and the refractive index.Intensity modulated detection method, structure are very simple, and the pick-up unit price is also very cheap, but the poor stability of system, can't distinguish and detect the light intensity signal variation is that error that caused by variations in refractive index or pick-up unit produces, detection system sensitivity is low.(4) phase modulation (PM): be scanned into firing angle θ earlier, seek the refractive index one surface plasma resonance incident angle regularly of sample, incident θ when fix resonance this moment, measure the resonance phase place under this refractive index then, when the variations in refractive index of sample, repeat said process, setting up resonates concerns between phase place and the refractive index, just can obtain the refractive index size of sample according to this relation.It has signal to noise ratio (S/N ratio) and higher detection sensitivity preferably, and shortcoming is the pick-up unit complexity of system, is difficult to realize the portability of surface plasma resonance sensor apparatus.

Angular modulation, wavelength-modulated and intensity modulated technology are very ripe, and these technology extensively adopt in surface plasma resonance (SPR) sensing testing instrument and designed monitoring analysis system.Angle adjusting method accuracy of detection height, but, make use in real time at the scene can introduce bigger noise owing to surface plasma resonance angular modulation method can't be distinguished the variations in refractive index of the liquid to be measured that the variation of environmental factor causes.

Summary of the invention

At the problems referred to above, technical program of the present invention lies in adopting a kind of portable biochemical analyser based on surface plasma resonance, by the main frame body, be arranged on the intrinsic micro-mainframe computer of main frame, biology sensor pick-up unit body, be provided with near-infrared luminous diode in the biology sensor pick-up unit body, polaroid, the line array CCD array detector, signal-transmitting cable and biochip, the line array CCD array detector is communicated with microcomputer by transmission cable, glass baseplate surface at biochip has the Au film, is fixed with bio-sensitive film on the Au film.

The thickness that the surface of the glass substrate of described biochip is coated with the Au film is 50nm.

Be provided with near-infrared luminous diode in the biology sensor pick-up unit body of the present invention, polaroid, the line array CCD array detector, signal-transmitting cable and biochip, glass baseplate surface at biochip has the Au film, be fixed with bio-sensitive film on the Au film, the incident beam that near-infrared luminous diode sends, arrive the surface of the glass substrate of biochip through polaroid, the superficial growth of glass substrate has the Au film, fixed biologically sensitive membrane on the Au film, because the sensitive membrane of measured matter and biomolecule reacts, cause the refractive index on Au film surface to change, produce surface plasma resonance (SPR), from the biochip beam reflected, CCD by linear array receives, the CCD of linear array changes light signal into electric signal, and electric signal sends to microcomputer by cable, and the result of calculation of the mutual relationship of the positional information that microcomputer detects CCD and the biological substance of variable concentrations shows on LCD.The invention provides a kind ofly based on the surface plasma resonance principle, the manual biology sensor pick-up unit of liquid feeding realizes that the surface plasma resonance biological instrument detects use at the scene; The angular modulation characteristics that kept surface plasma resonance biological sensor adopt line array CCD to come the detection of reflected spectral signal, are determined the position of resonance spectrum to have solved the manufacturing cost height that brings owing to angular modulation, the problem of system complex by line array CCD; In addition, the present invention also utilizes digital liquid crystal display displays biomolecule concentration to be analyzed, makes the surface plasma resonance Biochemical Analyzer more portable.

Description of drawings

Fig. 1 is a structural representation of the present invention;

Fig. 2 is the cut-open view of Fig. 1;

Fig. 3 is the structural drawing of biochip among Fig. 1.

Embodiment

As shown in Figure 1-Figure 3, in the present embodiment, portable biochemical analyser is by micro-mainframe computer 8, near-infrared luminous diode 9, polaroid 10, line array CCD array detector 11, signal-transmitting cable 12, LCD 4, main frame body 5, biochip 6, biology sensor pick-up unit body 7 is formed.The incident beam that near-infrared luminous diode 9 sends, arrive the surface of the glass substrate 1 of biochip 6 through polaroid 10, the superficial growth of glass substrate 1 has Au film 2, the thickness of Au film is 50nm, be fixed with bio-sensitive film 3 on the Au film 2, because the bio-sensitive film 3 of measured matter and biomolecule reacts, cause the refractive index on bio-sensitive film 3 surfaces to change, produce surface plasma resonance SPR, from biochip 6 beam reflected, receive by line array CCD array detector 11, line array CCD array detector 11 changes light signal into electric signal, electric signal sends to microcomputer 8 by cable 12, and the result of calculation of the positional information that microcomputer 8 detects line array CCD array detector 11 and the biological substance mutual relationship of variable concentrations shows on LCD 4.

It should be noted last that: above embodiment is the unrestricted technical scheme of the present invention in order to explanation only, although the present invention is had been described in detail with reference to the foregoing description, those of ordinary skill in the art is to be understood that: still can make amendment or be equal to replacement the present invention, and not breaking away from any modification or partial replacement of the spirit and scope of the present invention, it all should be encompassed in the middle of the claim scope of the present invention.

Claims (2)

1, a kind of portable biochemical analyser based on surface plasma resonance, it is characterized in that, by the main frame body, be arranged on the LCD that intrinsic micro-mainframe computer of main frame and surface thereof are provided with, biology sensor pick-up unit body, be provided with near-infrared luminous diode in the biology sensor pick-up unit body, polaroid, the line array CCD array detector, signal-transmitting cable and biochip, the line array CCD array detector is communicated with microcomputer by transmission cable, glass baseplate surface at biochip has the Au film, is fixed with bio-sensitive film on the Au film.

2, the portable biochemical analyser based on surface plasma resonance according to claim 1 is characterized in that, the thickness that the surface of the glass substrate of described biochip is coated with the Au film is 50nm.

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