CN101069508A - Farm chemical composition, preparing method and use - Google Patents
Farm chemical composition, preparing method and use Download PDFInfo
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- CN101069508A CN101069508A CN 200610026485 CN200610026485A CN101069508A CN 101069508 A CN101069508 A CN 101069508A CN 200610026485 CN200610026485 CN 200610026485 CN 200610026485 A CN200610026485 A CN 200610026485A CN 101069508 A CN101069508 A CN 101069508A
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Abstract
The present invention provides a pesticide composite. It contains meteloidine, tripterygium wilfordii lactone and toosendanin which are used as active components and carrier. Said invention also relates to its preparation method and application. The invented pesticide composite has broad spectrum insecticidal and germicidal function.
Description
Technical field
The present invention relates to pesticide field, particularly, the present invention relates to a kind of plant pesticide composition of novelty, prepare the method for this composition pesticide and the purposes of this composition pesticide.
Background technology
China is a large agricultural country, up to now still at the chemical pesticide that is extensive use of the synthetic that natural world do not have.Because natural world does not have corresponding micropopulation, causes residue of pesticide to degrade, contaminated land, water source and environment.Residue of pesticide enter people and animals through food chain, bring out multiple disease.Moreover the use of chemical pesticide has had half a century, and the pesticide resistance of insect becomes tens of times growth, has greatly restricted the sustainable development of China's agricultural.
Therefore, still press in this area a kind of novelty to harmless naturally biological pesticide composite.
Summary of the invention
For addressing the above problem, first aspect present invention provides a kind of composition pesticide, and datura total alkali, thunder godvine lactones, toosendanin that described composition pesticide comprises desinsection degerming effective dose are active component, and acceptable carrier on the agricultural chemicals.
The present invention also provides a kind of the present invention of preparation method of above-mentioned composition pesticide on the other hand, described method comprises acceptable carrier on the datura total alkali of desinsection degerming effective dose, thunder godvine lactones, toosendanin and the agricultural chemicals is mixed, and makes described composition pesticide.
The present invention also provides a kind of method that plant is carried out the desinsection degerming on the other hand, and this method comprises above-mentioned composition pesticide is applied on the plant.
The present invention also relates to above-mentioned composition pesticide on the other hand in the purposes of plant being carried out in the desinsection degerming.
Composition pesticide of the present invention is made of natural bioactivity substance, it does not contain original non-existent element in heavy metal and the human body, in addition, composition stable chemical performance of the present invention, raw material resources are abundant, be easy to artificial cultivation, do not have waste water, waste gas in process of production, the dregs of a decoction can fully utilize produces foliage fertilizer and medicine fertilizer.Said composition has the broad spectrum disinfestation degermation, in field trial, lepidoptera pests such as cabbage caterpillar, diamond-back moth, prodenia litura all there is killing rate more than 90% to 1～5 instar under 1: 1000 condition of thinner ratio, under the condition to 1: 1500 times of the larva thinner ratio below 3 instars, killing rate remains on more than 90%.Composition of the present invention has food refusal, tags, the stomach toxicity effect, and rapider than general vegetable insecticide onset, and the longevity of residure is more than 20 days.Said composition contains the nutrient of plant growth, Foliage Absorption after spraying, and crop is obvious general green grass or young crops after three days, and growing way is better than other crops of not spraying.Other purposes of the present invention and advantage can be learnt from following description.
Embodiment
A. composition
First aspect present invention provides a kind of composition pesticide, it is characterized in that, described composition pesticide comprises acceptable carrier on datura total alkali, thunder godvine lactones, toosendanin isoreactivity composition and the agricultural chemicals of desinsection degerming effective dose.
Amount that each active component exists combine kill pests and fungi effectively represented in term " desinsection degerming effective dose ".The insect that is fit to kill with composition pesticide of the present invention for example comprises, but be not limited to, Lepidoptera is diamond-back moth, cabbage caterpillar, prodenia litura, castor-oil plant noctuid, cigarette aphid noctuid, moths attracted by lamplight, tea geometrid, the flat abdomen stinkbug of beans, aulacophora femoralis etc. for example, Homoptera such as cotten aphid, melon aphid, black peach aphid, radish aphid rice leafhopper etc.; Two spotted spider mite on dipterous fly maggot and the fruit tree, tent caterpillar, steinernema etc.The fungi that is fit to kill with composition pesticide of the present invention for example includes, but are not limited to, and causes the fungi of diseases such as rotted leaf disease, graywall, white blister, stem rot, fusarium wilt, anthracnose, black spot, powdery mildew.After having determined the active component in the present composition, those skilled in the art is easy to result according to the desinsection degerming and determines each active component consumption at concrete insect or fungi.
In a preferable embodiment of the present invention, with the percentage by weight of the present composition is that benchmark calculates, and the toosendanin of thunder godvine lactones, 0.1～1% weight that contains datura total alkali, 0.01～0.2% weight of 0.5～6% weight in the composition pesticide of the present invention is as active component.In a better embodiment, with the percentage by weight of the present composition is that benchmark calculates, and contains 1～5% datura total alkali, 0.02～0.1% thunder godvine lactones, 0.2～0.6% toosendanin in the composition pesticide of the present invention as active component.
The implication of used datura total alkali, thunder godvine lactones, toosendanin all is known to those skilled in the art in the present composition.The datura total alkali is based on hyoscine, and all the other are hyoscyamine and atropine.Hyoscine, hyoscyamine and atropinic molecular formula, molecular weight structural formula are as follows.
Hyoscine: molecular formula C
17H
21NO
4, molecular weight 303.35, structural formula is as follows:
Hyoscyamine: (levo form) molecular formula C
17H
23NO
3, molecular weight 289.36, structural formula is as follows:
Atropine: (raceme) molecular formula C
17H
23NO
3, molecular weight 289.36, structural formula is as follows:
Under normal conditions, the datura total alkali is by the hyoscine of 40-80% (weight), and the atropine of the hyoscyamine of 10-30% (weight) and 10-30% (weight) is formed.Because the difference of extracting method, the composition of datura total alkali may be variant slightly with respect to above-mentioned composition.Yet, it should be recognized by those skilled in the art that these variations can't influence the realization of goal of the invention of the present invention.In other words, form content not the datura total alkali in above-mentioned normal conditions scope also within limit of consideration of the present invention.
The thunder godvine lactones has another name called triptolide, and molecular weight is 360.39, molecular formula C
20H
24O
6, structural formula is as follows:
The molecular weight 574.60 of toosendanin (furans triterpene), molecular formula C
30H
38O
11, its structural formula is as follows:
Datura total alkali among the present invention (comprise its respectively form hyoscine, hyoscyamine, atropine) and thunder godvine lactones and toosendanin all can be buied from commercial channels.For example hyoscine, hyoscyamine can be buied from mountains and rivers, Xi'an Bioisystech Co., Ltd, atropine can be buied from Shanghai Hefeng Pharmaceutical Co., Ltd., the thunder godvine lactones can be buied from Huangshi three nine-day periods after the winter solstice pharmaceutical Co. Ltd, and toosendanin can be buied from De Libang Science and Technology Ltd. of Shaanxi agricultural university.The datura total alkali for example can be available from Lanzhou medical college and Jinchang City Study of Medicinal Herbs institute.
In preferable embodiment, said components also can adopt organic solvent extraction technology to extract acquisition from the plant (for example datura, henbane seed, belladonna, datura flower, Tang Gute henbane, scopolia acutangula, velamen of Tripterygium wilfordii, Fructus meliae toosendan etc.) of being rich in datura total alkali, thunder godvine lactones, toosendanin by those skilled in the art.
In preferable embodiment, described extraction process may further comprise the steps: datura flower or datura meal, thunder godvine meal, Fructus meliae toosendan meal water or organic solvent (as ethanol, acetone etc.) are extracted and extract, use then such as means such as filtrations and remove slag, concentrating under reduced pressure is removed solvent.In preferable embodiment, " organic solvent extraction " is to adopt the normal temperature and pressure Continuous Countercurrent Extraction, and concentrated normally vacuum decompression concentrates, and temperature is below 45 ℃.In the said extracted scheme, preferable organic solvent is an ethanol.In better technical scheme,, be to adopt Extraction by Ultrasound for datura total alkali and thunder godvine lactones.Usually, adopted the 20KHz-36KHz ultrasonic Extraction 20-40 minute, this method improves 5%～10% than the conventional alkaloid that extracted 3 hours, and has saved time and solvent consumption greatly.Whole process of preparation is a complete closed in the above-described embodiment, and the purification of effective active composition is adopted according to molecular weight, polarity, acid-base value, solvability size and carried out system's separation.
Hereinafter enumerated multiple extraction process in the implementation example.Yet, should be appreciated that scope of the present invention is not limited to these concrete schemes.Those skilled in the art can adopt other conventional extraction schemes that the plant of being rich in datura total alkali, thunder godvine lactones, toosendanin is extracted processing fully.
In technical scheme of the present invention, term " acceptable carrier on the agricultural chemicals " has the implication the most widely known to those skilled in the art, and it can comprise following optional component: additive such as adjuvant, excipient, wetting agent, surfactant, dispersant, dyestuff, thixotropic agent, synergist, binder.The kind of carrier and consumption can be by those skilled in the art according to the forming of composition, and factors such as the consumption of active component are determined.
Term used herein " contains " in the expression composition of the present invention can also contain some other component of not pointing out.Therefore, except above-mentioned carrier, composition of the present invention can also randomly comprise stable material, the medicine of including in other insecticides, miticide, plant nematocide, pest repellant, attractant or pheromone etc. and NF and the local pharmacopeia.These can design as required to improve and tire, or make composition bring into play other useful functions in same processing region.
In a preferable embodiment, composition of the present invention is made up of acceptable carrier on datura total alkali, thunder godvine lactones, toosendanin isoreactivity composition and the agricultural chemicals of desinsection degerming effective dose basically.In another embodiment, composition of the present invention is made up of acceptable carrier (preferably water) on datura total alkali, thunder godvine lactones, toosendanin isoreactivity composition and the agricultural chemicals of desinsection degerming effective dose.Herein, " basically by ... form " in the expression composition of the present invention except containing main active component datura total alkali, thunder godvine lactones, toosendanin, also can comprise some for the desinsection degerming characteristic of the composition component of influence in fact not.Term used herein " by ... form " represent that then composition of the present invention is made up of pointed concrete component, do not have other components, but it can have the impurity of content in common scope.
Composition of the present invention can be the form of gel, dispersant, emulsion or solution, wherein randomly mixes various wetting agents, dispersant, emulsifier or gelling agent.Dispersant and emulsifier comprise sulforicinoleic acid fat, quaternary ammonium derivative or product based on the condensation product of going back oxidative ethane and nonyl alcohol and octanol, or by with go back the oxidative ethane condensation and make free hydroxyl etherificate have the carboxylic esters of deliquescent sorbitan and the mixture of the reagent of these types.These gels, emulsion, suspension, dispersion liquid and/or solution can make with water-based, organic or water-based-organic diluent, these thinners for example are acetophenone, isophorone, toluene, dimethylbenzene, mineral oil, animal oil or vegetable oil, and water-soluble polymer (and mixture of these thinners), they can contain wetting agent, dispersant or emulsifier or its mixture of ion or nonionic, those for example above-mentioned types.In preferable experimental program, composition of the present invention adopts the form of the aqueous solution, that is, described carrier is a water.
B) preparation of compositions method
Composition pesticide of the present invention can by with the commercially available hyoscine of buying, hyoscyamine, atropine, thunder godvine lactones, toosendanin in certain proportion with agricultural chemicals on acceptable carrier mix easily and make, the mixing order of each component has no particular limits.
In view of raw material resources such as datura, thunder godvine, Fructus meliae toosendan very abundant, in preferable embodiment of the present invention, can adopt raw material to extract with organic solvent to datura or datura flower, thunder godvine, Fructus meliae toosendan, the method of filtering and concentrating purification obtains datura total alkali, thunder godvine lactones, toosendanin then, mix acceptable carrier is composite on above-mentioned active principle and the agricultural chemicals with certain proportion then, thereby make composition pesticide of the present invention.
C) use
Composition described herein and method can be used to protect economic crops such as vegetables, melon and fruit, flowers, tealeaves, forest, Chinese herbal medicine.By the synergy between datura total alkali, thunder godvine lactones, the toosendanin three, the composition of different proportion of the present invention and concentration can be killed various insect colony effectively, these insect colonies can be adult, larva and the ovum of Lepidoptera (butterfly and moth), for example diamond-back moth, cabbage caterpillar, prodenia litura, castor-oil plant noctuid, cigarette aphid noctuid, moths attracted by lamplight, tea geometrid, the flat abdomen stinkbug of beans, aulacophora femoralis etc.; Can be cotten aphid, melon aphid, black peach aphid, the radish aphid rice leafhopper of Homoptera; Can be two spotted spider mite, tent caterpillar, steinernema on dipterous fly maggot or the like and the fruit tree etc.
Composition described herein and method also have good preventive and therapeutic effect to fungus diseases such as rotted leaf disease, graywall, white blister, stem rot, fusarium wilt, anthracnose, black spot, powdery mildews.
Therefore, the present invention relates to a kind of method that plant is carried out the desinsection degerming on the other hand, and this method comprises above-mentioned composition pesticide of the present invention is applied on the plant.The not special restriction in the position that applies.Preferred methods is that composition pesticide of the present invention is applied on the plant leaf surface.Apply and to adopt any routine techniques of knowing in this area, for example adopt that spraying is sprayed etc. and apply mode in the air.Before applying, can dilute composition of the present invention as required, for example, extension rate is preferably 100 to 5000 times.In addition, those skilled in the art need not can determine the suitableeest dispenser dosage of the present invention through too much test that for example, preferable dispenser dosage is in 1000～2000 times of scopes.
Below in conjunction with specific embodiment, further set forth the present invention.Should understand these embodiment only is used to the present invention is described and is not used in and limit the scope of the invention.Unless have in addition refer in particular to or capable territory in outside the conventional implication of knowing, the percentage among the embodiment is all represented percentage by weight.
The extraction process of embodiment 1 datura total alkali
Carry out following each extracting method respectively.
1) datura flower (datura alba Nees) meal adds 40 ℃ of backflows of 95% ethanol 3 * 1 hours/time, reclaim ethanol to concentrate (solid-liquid ratio is 1: 2), use chloroform eccysis oil-soluble impurities (acidifying 0.5mol/l aqueous sulfuric acid) after the acidifying, the acid liquid that obtains is neutralized to pH 9 with sodium carbonate and uses chloroform extraction again, extract reclaims chloroform to doing, and can obtain oily datura total alkali.According to first (appendix VI0) high performance liquid chromatography of Pharmacopoeia of the People's Republic of China version in 2000 the datura total alkali in the described oily datura total alkali is measured, recorded 0.25%～0.30% (weight) of former medicine (the being the datura flower meal) amount of gained datura total alkali.
2) datura (seed) meal adds (60-90) petroleum ether degreasing.The datura meal of degreasing adds 95% ethanol and refluxed 3 * 1 hours/time for 40 ℃, reclaiming ethanol to concentrate is medicinal extract, staticly settle after being dissolved in water, getting supernatant adds ammoniacal liquor and transfers to more than the ph10, refluxed 2 * 30 minutes/time with carbon tetrachloride again, reclaim carbon tetrachloride, can obtain the datura total alkali, wherein 0.30%～0.35% of the former dose of gained datura total alkali.
3) datura (seed) meal adds (60-90) petroleum ether degreasing.The datura meal of degreasing adds 30 ℃ of 95% ethanol and adopts the ultrasonic processing of 20KHz～36KHz 30 minutes, filters the back and reclaims ethanol to medicinal extract, is oily datura total alkali, and wherein 0.30%～0.38% of the former dose of gained datura total alkali.
All can from the flower of datura alba Nees, hair leaf datura, datura, leaf, branch, seed, extract the datura total alkali with said method.From agents area, the content of flower is greater than leaf and seed, and the content of leaf is a bit larger tham seed, but the seed price is minimum.Said method all uses the same position of datura to be used as medicine, and adopts the datura total alkali technology of Extraction by Ultrasound easy, and yield is the highest, and is generally high by 5%～10% than other method.
The extraction of embodiment 2. thunder godvine lactones
1) velamen of Tripterygium wilfordii meal cold water soak is 2 hours, and decocting boils 3 * 2h/ time, and the condensed water decocting liquid adds ethanol to containing alcohol amount 75% to solid-liquid ratio 1: 2 after the cooling then, places after 5 hours and filters, recovery filtrate.Sediment merges cleaning solution and filtrate with 75% washing with alcohol 3 times, reclaims ethanol to paste.Measure the content of triptolide according to first (appendix VI0) high performance liquid chromatography of Pharmacopoeia of the People's Republic of China version in 2000, recording the gained triptolide is 0.03%～0.08% (weight) of former dose.
2) velamen of Tripterygium wilfordii meal cold water soak is 2 hours, adds hydrochloric acid and transfers pH=4, adds 0.5% cellulase (unit of activity 2000u/g) and fully stirs, and 40 ℃ were refluxed 1.5 hours, adds 5 times of amount 95% alcohol refluxs again and extracts 1 hour, filters, and soup is standby.The dregs of a decoction add 5 times of amount 60% alcohol refluxs and extracted 0.5 hour, filter, and the dregs of a decoction discard, and merge the secondary soup, reclaim ethanol to there not being the alcohol flavor, and the centrifuging and taking supernatant with absolute ethyl alcohol extraction 3 * 30 minutes/time, reclaims ethanol to doing.The gained triptolide is 0.05%～0.1% of a former dose.
3) velamen of Tripterygium wilfordii meal cold water soak is 2 hours, adds hydrochloric acid and transfers pH=4, adds 0.5 cellulase (unit of activity 2000v/g) and fully stirs, handled 30 minutes 30 ℃ of Ultrasonic K Hz20～36, add 30 ℃ of ultrasonic processing of 5 times of amount 80% ethanol 30 minutes again, filter and discard the dregs of a decoction, filtrate recycling ethanol is to doing.The gained triptolide is 0.05%～0.15% of a former dose.
Said method all can extract the thunder godvine lactones from velamen of Tripterygium wilfordii.The pharmaceutical purity cleanliness that the third method technology is simple, the time short, extract is good, and content improves.
The extraction of embodiment 3. toosendanins
1) Fructus meliae toosendan (or melia toosendan skin) meal filters with 30% alcohol reflux 4 * 1 hours/time, and filtrate is with wet carbon diafiltration absorption, flow velocity 80～100ml/ minute, wet carbon amount be 2～3 times of material quantity repeatedly diafiltration to percolate do not have obvious bitter taste.Wet carbon 95% ethanol elution flow velocity 20～40ml/ minute, is eluted to the eluent water white transparency.Merge the eluent decompression recycling ethanol, concentrate leaves standstill filtering-depositing, takes out supernatant liquor and gets yellow powder, washes with 95% ethanol and filters the toosendanin that alumina column obtains white or little Huang.According to first (appendix VI0) high effective liquid chromatography for measuring toosendanin of Pharmacopoeia of the People's Republic of China version in 2000 content, recording former dose is 0.3% (weight) of extract.
2) Fructus meliae toosendan (or melia toosendan skin) is with (60～90) petroleum ether degreasing, and the degreasing meal is with 40 ℃ of 95% ethanol
Refluxed 4 * 1 hours/time, 1/5 95% ethanol with former dose filters at every turn, removes slag.Decompression filtrate recycling ethanol, residue add the saturated extracting n-butyl alcohol 6 * 0.5 hours/time of former dose 1/2, and extract recovered under reduced pressure n-butanol promptly gets yellow toosendanin.0.2%～0.3% of the former dose of toosendanin content.
3) Fructus meliae toosendan (or melia toosendan skin) is with (60～90) petroleum ether degreasing, and the degreasing meal is with 30 ℃ of 95% ethanol
The ultrasonic processing in KHz36～50 50 minutes is filtered, and discards the dregs of a decoction; Decompression filtrate recycling ethanol is to paste, adds the ultrasonic processing of saturated n-butanol 30 minutes of 4 times of former doses, filters, and the recovered under reduced pressure n-butanol gets final product.0.3%～0.4% of the former dose of toosendanin content.
The said extracted method, the third technology is simple, and the time significantly shortens, and degree of purity and content all are better than second kind, and ratio content is suitable mutually with first kind, but the technology cost descends.
The preparation of embodiment 4. compositions
Earlier hyoscine, hyoscyamine, the atropine of buying being mixed with datura total alkali configuration proportion is that hyoscine is 70% (weight), and hyoscyamine, atropine respectively are 15% (weight).The datura total alkali that is mixed with is ground to form fine powder, be dissolved in 95% ethanol, (hyoscine of buying, hyoscyamine, atropine are the tablet of content 0.5mg to the elimination sediment, necessary elimination carrier), and measure peak value through high performance liquid chromatograph as reference substance, the datura total alkali that embodiment 1 is extracted dissolves in 95% ethanol equally as test sample then.Measure peak value through high performance liquid chromatograph, compare the two collection of illustrative plates of measuring thereafter, promptly determine the content of the datura total alkali that the present invention extracts.Triptolide, toosendanin are buied the back and are measured by last example, in addition the triperygium wilfordii extractive, the Sichuan chinaberry extract that extract are measured by last example as test sample, relatively draw the content of triptolide and toosendanin in the extract behind the collection of illustrative plates.Concrete content assaying method is according to first (appendix VI0) high performance liquid chromatography of Pharmacopoeia of the People's Republic of China version in 2000.
Then, the carrier (water) of the datura extract, triperygium wilfordii extractive, Sichuan chinaberry extract and the surplus that are obtained among the embodiment 1,2,3 is mixed, be mixed with desinsection of the present invention and remove bacteria composition.The amount of preparation of each extract (weight %) (in the amount of the datura total alkali that wherein contains, Triptolide, toosendanin) is as shown in the table:
Table 1
| Composition no | The datura total alkali | Triptolide | Toosendanin |
| 1 | 4 | 0.06 | 0.1 |
| 2 | 3 | 0.06 | 0.5 |
| 3 | 2 | 0.1 | 0.5 |
| 4 | 2 | 0.15 | 0.3 |
In addition, buy from commercial channels datura total alkali, thunder godvine lactones, (hyoscine of buying from commercial channels, hyoscyamine, atropine are content 0.5mg/ sheet to toosendanin.Triptolide is the 33ug/ sheet, and toosendanin content is 0.6% missible oil), according to ratio shown in the following table 1 (continuing), it is mixed with the carrier (water) of surplus, make composition with following weight (%).
Table 1 (continuing)
| Composition no | The datura total alkali | Triptolide | Toosendanin |
| 5 | 5 | 0.06 | 0.12 |
| 6 | 4 | 0.08 | 0.5 |
| 7 | 4 | 0.1 | 0.5 |
| 8 | 3 | 0.1 | 0.8 |
Embodiment 5 field trials
The inventor in Shanghai, ground such as Mingguang City, Anhui, Quanjiang, Quanzhou, Fujian, the insecticidal effect of the present composition (numbering 3 and numbering 7) that embodiment 4 is made is measured, the details comprehensive report of relevant test is as follows:
(1) test method and object:
According to pertinent regulations and the step of agricultural sector, test at treatment region and check plot with spray-on process about " pesticide efficacy field test request ".Examination is murdered worm and is mainly contained diamond-back moth, prodenia litura, cabbage caterpillar, aphid, tea geometrid, yellow tea mite, dish miller, beans three-spotted plusia etc., and chief crop is wild cabbage, tealeaves, radish, green vegetables, French beans etc.To check the disease situation of change of insect survival condition and plant leaf surface cane in 24,48,72 hours.
Obtaining of test data mainly adopts Abbott ' s formula to calculate the lethality and the corrected mortality of each concentration of treatment.
Lethality (%)=(borer population of living behind examination borer population-medicine)/examination borer population * 100
Corrected mortality (%)=(chemicals treatment lethality-contrast lethality)/(100-contrasts lethality) * 100
(2) result and analysis
The field trial drug effect of insects such as composition for preventing and controlling diamondback moth larvae of the present invention sees the following form 2.
Table 2: the drug effect of control diamond-back moth (spraying is handled)
| Numbering | Concentration (doubly) | Worm age | 24Hr lethality (%) | 48Hr lethality (%) | 72Hr lethality (%) |
| 1 | 500 | 5 | 88.6 | 100 | 100 |
| 2 | 1000 | 3 | 75.4 | 96.8 | 100 |
| 3 | 1500 | 3 | 72 | 97.87 | 100 |
| 4 | 2000 | 3 | 66.5 | 89.6 | 92 |
Data in the table are 3 groups of test mean values
Analyze: present composition control diamond-back moth, the result shows: very obvious to the diamond-back moth lethality below 3 ages under 1: 500～2000 the concentration, better ratio is under 1: 1000～1500 the concentration, and is more excellent to the diamond-back moth control efficiency below 5 ages.
Table 3: the drug effect of control radish aphid (spraying is handled)
| Numbering | Concentration (doubly) | Worm age | 24Hr lethality (%) | 48Hr lethality (%) | 72Hr lethality (%) |
| 1 | 1500 | Become | 78 | 84 | 92.7 |
| 2 | 1800 | Become | 67 | 77 | 90.1 |
Data in the table are 4 groups of test mean values
Table 4: the drug effect (spraying is handled) of control beans three-spotted plusia, French beans plume moth
| Numbering | Concentration (doubly) | Worm age | 24Hr lethality (%) | 48Hr lethality (%) | 72Hr lethality (%) |
| 1 | 1000 | Adult | 100 | / | / |
| 2 | 1500 | Adult | 100 | / | / |
Data are 4 cell means in the table.In actual observation, above-mentioned insect is handled back 4 hours lethality in spraying and just reaches more than 90%.
Table 5: the drug effect of control tea geometrid, yellow tea mite (spraying is handled)
| Numbering | Concentration (doubly) | Worm age | 24Hr lethality (%) | 48Hr lethality (%) | 72Hr lethality (%) |
| 1 | 1500 | Adult | 100 | 100 | / |
| 2 | 2000 | Adult | 84 | 93.2 | 95.7 |
Data are 4 cell means in the table.
From in mid-September, 2005～early November, the inventor successively handles the disease spraying of cucumber, wild cabbage, Chinese cabbage, radish, strawberry again with the present composition of same experimental concentration.As wild cabbage graywall, stem rot, the fusarium wilt of cucumber, anthracnose, the black spot of radish, the powdery mildew of strawberry, verticillium wilt etc. all obtain comparatively desirable effect, sprayed the sick leaf of handling, generally can both see original blade face that corroded by disease after three days, thalline begins death and comes off, branches and leaves are turned green shinny gradually, and growing way takes a turn for the better.
In the test of control cabbage caterpillar, contrast at last with the simple insecticidal effect, result such as following table 6 (with three age cabbage caterpillar be example).
Table 6
| The medicine name | Concentration % | Lethality % | Concentration % | Lethality % |
| The datura total alkali | 0.5～1.5 | ＜64 | 2～3 | ＞90 |
| The thunder godvine lactones | 0.03～0.05 | ＜78 | 0.05～0.08 | ＞78＜88.4 |
| Toosendanin | 0.3％ | ＜60％ | 0.5％ | 86 |
The simple sample insecticidal effect that simple and market are purchased is variant slightly, and comparatively speaking, the simple that the present invention extracts is better than that market purchases, and wherein reason is that extract has more pharmacologically active than pure product.Simple is poorer than compound composition prepared insecticidal effect in addition, and the desinsection kind is few.
Although the invention describes object lesson, having a bit is significantly for a person skilled in the art, promptly under the premise without departing from the spirit and scope of the present invention, can do various variations and change to the present invention.Therefore, claims have covered all these changes within the scope of the present invention.It is for referencial use that this paper is all included in all publications, patent and the patent application that this paper quotes in.
Claims (10)
1. a composition pesticide is characterized in that, datura total alkali, thunder godvine lactones, toosendanin that described composition pesticide comprises desinsection degerming effective dose are active component, and acceptable carrier on the agricultural chemicals.
2. composition pesticide according to claim 1 is characterized in that acceptable carrier is a water on the described agricultural chemicals.
3. composition pesticide according to claim 1 is characterized in that, described composition pesticide contains the toosendanin of thunder godvine lactones, 0.1～1% weight of datura total alkali, 0.01～0.2% weight of 0.5～6% weight as active component.
4. according to each described composition pesticide of claim 1-3, it is characterized in that described datura total alkali is by the hyoscine of 40-80% weight, the atropine of the hyoscyamine of 10-30% weight and 10-30% weight is formed.
5. composition pesticide according to claim 1 is characterized in that, described composition pesticide is made up of acceptable carrier on datura total alkali, thunder godvine lactones, toosendanin and the agricultural chemicals of desinsection degerming effective dose.
6. method for preparing the described composition pesticide of claim 1, it is characterized in that, described method comprises acceptable carrier on the datura total alkali of desinsection degerming effective dose, thunder godvine lactones, toosendanin and the agricultural chemicals is mixed, and makes described composition pesticide.
7. method as claimed in claim 6 is characterized in that, described datura total alkali, thunder godvine lactones, toosendanin extract from datura or datura flower, thunder godvine, Fructus meliae toosendan respectively as organic solvent with ethanol and obtain.
8. method as claimed in claim 7 is characterized in that, described extracting method adopts Extraction by Ultrasound.
9. method that plant is carried out the desinsection degerming, this method comprise the described composition pesticide of claim 1 are applied on the plant.
10. the described composition pesticide of claim 1 is in the purposes of plant being carried out in the desinsection degerming.
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Cited By (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102239891A (en) * | 2011-05-30 | 2011-11-16 | 杨凌农科大无公害农药研究服务中心 | Enhanced compound pesticide containing toosedarin plant source and preparation method thereof |
| CN101638580B (en) * | 2009-08-26 | 2012-08-08 | 广东省食品工业研究所 | Method for preparing rosemary natural anti-oxidant |
| CN102696700A (en) * | 2012-06-12 | 2012-10-03 | 刘倚乐 | Chinese herbal medicine mosquito and fly killing agent |
| CN104365691A (en) * | 2014-11-19 | 2015-02-25 | 沈阳化工研究院有限公司 | Botanical pesticide suspension agent as well as preparation method and application of botanical pesticide suspension agent |
| CN104430609A (en) * | 2014-11-19 | 2015-03-25 | 沈阳化工研究院有限公司 | Botanic pesticide water dispersant, preparation method and application thereof |
| CN105961458A (en) * | 2016-05-17 | 2016-09-28 | 梁文荣 | Special herbicide for grassy weeds |
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2006
- 2006-05-12 CN CN 200610026485 patent/CN101069508A/en active Pending
Cited By (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101638580B (en) * | 2009-08-26 | 2012-08-08 | 广东省食品工业研究所 | Method for preparing rosemary natural anti-oxidant |
| CN102239891A (en) * | 2011-05-30 | 2011-11-16 | 杨凌农科大无公害农药研究服务中心 | Enhanced compound pesticide containing toosedarin plant source and preparation method thereof |
| CN102696700A (en) * | 2012-06-12 | 2012-10-03 | 刘倚乐 | Chinese herbal medicine mosquito and fly killing agent |
| CN104365691A (en) * | 2014-11-19 | 2015-02-25 | 沈阳化工研究院有限公司 | Botanical pesticide suspension agent as well as preparation method and application of botanical pesticide suspension agent |
| CN104430609A (en) * | 2014-11-19 | 2015-03-25 | 沈阳化工研究院有限公司 | Botanic pesticide water dispersant, preparation method and application thereof |
| CN105961458A (en) * | 2016-05-17 | 2016-09-28 | 梁文荣 | Special herbicide for grassy weeds |
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Application publication date: 20071114 |