CN101066471A - Cell-eliminating coanea matrix and its prepn process - Google Patents
Cell-eliminating coanea matrix and its prepn process Download PDFInfo
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- CN101066471A CN101066471A CNA2007100276986A CN200710027698A CN101066471A CN 101066471 A CN101066471 A CN 101066471A CN A2007100276986 A CNA2007100276986 A CN A2007100276986A CN 200710027698 A CN200710027698 A CN 200710027698A CN 101066471 A CN101066471 A CN 101066471A
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61L—METHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS IN GENERAL; DISINFECTION, STERILISATION OR DEODORISATION OF AIR; CHEMICAL ASPECTS OF BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES; MATERIALS FOR BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES
- A61L27/00—Materials for grafts or prostheses or for coating grafts or prostheses
- A61L27/36—Materials for grafts or prostheses or for coating grafts or prostheses containing ingredients of undetermined constitution or reaction products thereof, e.g. transplant tissue, natural bone, extracellular matrix
- A61L27/38—Materials for grafts or prostheses or for coating grafts or prostheses containing ingredients of undetermined constitution or reaction products thereof, e.g. transplant tissue, natural bone, extracellular matrix containing added animal cells
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61L—METHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS IN GENERAL; DISINFECTION, STERILISATION OR DEODORISATION OF AIR; CHEMICAL ASPECTS OF BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES; MATERIALS FOR BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES
- A61L27/00—Materials for grafts or prostheses or for coating grafts or prostheses
- A61L27/36—Materials for grafts or prostheses or for coating grafts or prostheses containing ingredients of undetermined constitution or reaction products thereof, e.g. transplant tissue, natural bone, extracellular matrix
- A61L27/3604—Materials for grafts or prostheses or for coating grafts or prostheses containing ingredients of undetermined constitution or reaction products thereof, e.g. transplant tissue, natural bone, extracellular matrix characterised by the human or animal origin of the biological material, e.g. hair, fascia, fish scales, silk, shellac, pericardium, pleura, renal tissue, amniotic membrane, parenchymal tissue, fetal tissue, muscle tissue, fat tissue, enamel
- A61L27/3633—Extracellular matrix [ECM]
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61L—METHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS IN GENERAL; DISINFECTION, STERILISATION OR DEODORISATION OF AIR; CHEMICAL ASPECTS OF BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES; MATERIALS FOR BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES
- A61L27/00—Materials for grafts or prostheses or for coating grafts or prostheses
- A61L27/36—Materials for grafts or prostheses or for coating grafts or prostheses containing ingredients of undetermined constitution or reaction products thereof, e.g. transplant tissue, natural bone, extracellular matrix
- A61L27/3641—Materials for grafts or prostheses or for coating grafts or prostheses containing ingredients of undetermined constitution or reaction products thereof, e.g. transplant tissue, natural bone, extracellular matrix characterised by the site of application in the body
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61L—METHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS IN GENERAL; DISINFECTION, STERILISATION OR DEODORISATION OF AIR; CHEMICAL ASPECTS OF BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES; MATERIALS FOR BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES
- A61L2430/00—Materials or treatment for tissue regeneration
- A61L2430/16—Materials or treatment for tissue regeneration for reconstruction of eye parts, e.g. intraocular lens, cornea
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Abstract
The present invention discloses one kind of cell-eliminating cornea matrix, which consists of mainly hundreds of parallel collagen fiber plate layers with voids formed through eliminating cells in between. The cell-eliminating cornea matrix is used in repairing, reconstructing and shaping surficial eye defect, treating surficial eye diseases and constructing tissue engineering cornea. After being transplanted, the cell-eliminating cornea matrix may exist for long term or degrade gradually, and is high transparent. It may allow receptor nerve to grow into and receptor cell to attach on, and has the features of low immunogenicity and high tissue compatibility.
Description
Technical field
The invention belongs to medical instruments field, specifically relate to a kind of cell-eliminating coanea matrix and preparation method thereof.
Background technology
Corneal transplantation is treatment because of one of the keratopathy that infects, injures the various causes of disease such as congenital anomaly outward and cause, damaged, muddy, important method that diopter is unusual, owing to the donor source scarcity can not be treated many patients timely and effectively.The artificial cornea that synthetic material is made is because biocompatibility is poor, be difficult to after the transplanting and near the eyes tissue merge, easily produce and take off mistake, leak and complication such as front and back film.
Pterygium is a kind of common eye surface diseases, has the postoperative characteristics of recurrence easily, and simple pterygium excision associating limbal transplantation can reduce the postoperative recurrence rate of this disease.But present donor material deficiency.
The eye table comprises cornea, conjunctival epithelium and three parts of tear film.The treatment of the eye surface diseases that causes at different reasons promptly comprises effectively keeping and rebuilding these three aspect health.
Corneal refractive power accounts for 2/3 of eyeball total dioptric power, and the refractive status that changes eye with operation method can be used for correcting dissimilar ametropia.
The timbering material of organizational project plays a part nutrition to be provided, the shape size of place and decision institute repair tissue is provided for cell proliferation for cell.Natural macromolecular material such as collagen, hyaluronic acid, chitin and derivant thereof and its mechanical property of polysaccharide polymer substance are not good enough, and hydrophobicity is too strong, and it is too fast to degrade, and are unfavorable for that the cytotrophy material is to the material internal diffusion.The macromolecular material of synthetic (as polylactic acid, polyglycolic acid) etc. also is used for the carrier as tissue engineering comea, its transparency and less stable, and aseptic inflammatory reaction rate is higher, is unsuitable for clinical trial.Though amnion tissue can provide continuous basement membrane, contain collagen, laminin, fibronectin and various integral protein, these compositions are beneficial to epithelial differentiation and divide a word with a hyphen at the end of a line, because of the too thin reparation that is not suitable for corneal stroma deep layer pathological changes of material itself.
Summary of the invention
The technical issues that need to address of the present invention provide a kind of cell-eliminating coanea matrix, this cell-eliminating coanea matrix is used to repair that a table organization is damaged, reconstruction and moulding eye table organization, treatment eye surface diseases and as the carrier that makes up engineered biological cornea, in the hope of the source that affords ample material for the operation of clinical eye table.
A kind of cell-eliminating coanea matrix mainly is made of hundreds of the collagen fiber flaggies that are parallel to each other, and has cell to be sloughed the space that the back forms between the collagen fiber flaggy.
Described cell-eliminating coanea matrix, its basis comprise collagen fiber, corneal stroma (comprising sticking polyprotein and glycoprotein etc.) and take off cell and handle back residual after birth and endochylema composition.
The thickness of described cell-eliminating coanea matrix is preferably 0.01～5.0mm.
Described cell-eliminating coanea matrix is preferably the circle that diameter is 1mm～40mm.
Described cell-eliminating coanea matrix can also carry medicine and/or cytokine.
The preparation method of above-mentioned cell-eliminating coanea matrix, taking xenogenesis (animal class) or allogeneic corneal stroma is raw material, handles through the past cell, obtains described cell-eliminating coanea matrix.
Go cell to handle can to adopt cytopheresis (as filter, centrifugal, sedimentation etc.), also can adopt cell breakage method (the osmotic pressure impact of, physics infiltration molten etc., freeze-thawing method etc.) as acid-alkali treatment, chemical reagent processing, the enzyme of the high-pressure homogenization of Mechanical Crushing, pearl mill, ultrasonication etc., chemistry and biochemistry infiltration.
Preferably, go cell to be treated to the chemosmosis method, concrete steps are as follows: with concentration is that 0.05～5% anion surfactant SDS solution was handled hetero stroma of cornea 4～24 hours under 4～50 ℃ condition.This method does not constitute the destruction of corneal 3-D solid structure and matrix components thereof when removing the cornea tissue cell fully, make the most important characteristic of cornea-transparency and toughness be able to abundant reservation.
Go cell to handle and can also be another kind of chemosmosis method, step is as follows: with concentration is that 0.01～5% detergent TritonX-100 solution was handled hetero stroma of cornea 24～48 hours under 4～50 ℃ condition, afterwards with trypsinization 2-12 hour.
Described cell-eliminating coanea matrix can also be taked to remove heteroantigen and handle, the method of removing the heteroantigen processing can adopt primary bioseparation technology (as membrance separation, extraction, precipitation fractionation and foam separation etc.), also can adopt other biological isolation technics (as absorption, chromatograph, electrophoresis and electrochromatography etc.).
Preparing cell-eliminating coanea matrix of the present invention and can adopt xenogenesis (animal class) or allochthonous part cornea, can be whole corneas also, even comprises sclera, conjunctiva that part is attached thereto.Can be flesh tissue, also can be the tissue through preserving.Under certain action condition, (as: handle) through enzyme processing, rupture of membranes agent, slough the cell component in the material fully, remove in-house pathogenic microorganism to greatest extent, reduce or eliminate the antigenicity of tissue, but keep the three dimensional structure that its normal matrix fiber is arranged.
The corresponding cell of area load by the cell-eliminating coanea matrix made in the present invention can construct engineered biological cornea.Because cell-eliminating coanea matrix has certain rigidity and toughness, therefore have machinability, can form 3-D solid structure; Can be processed into different shape, as: circle, crescent etc.Can be to have certain radian, also can be plane.Can be made into receptor eye to be repaired yet and show the corresponding to thickness of damaged thickness, be processed as equal thickness, then cell-eliminating coanea matrix does not have diopter, if be processed as not equal thickness, just can have corresponding diopter.
Can long-term existence or progressively degraded after cell-eliminating coanea matrix of the present invention is transplanted, and being the highly transparent state, the nerve with receptor can be grown into, recipient cell can be in its long-term apposition growth in surface, immunogenicity is low and histocompatibility is good characteristics; In the time of can also or using rehydration in fabrication and processing, make it carry related drugs and/or cytokine, play the effect that new vessels is grown into and/or treated primary disease that suppresses; In nerve can be grown into substrate after transplanting, the epithelial cell of receptor can be at the stromal surface apposition growth; Described cell-eliminating coanea matrix also has the similar heat stability to receptor eye table organization.
Cell-eliminating coanea matrix preparation method of the present invention is simple, easy to operate, and prepared cell-eliminating coanea matrix has the following advantages:
1. come from biomaterial, have good support effect, can be used as safe and effective biologic material and implant ophthalmic for a long time.
2. can be used to repair the limbus of corneae that due to illness becomes excision, alleviate the insufficient contradiction of donor material.
The band conjunctiva take off cell angle conjunctiva substrate, promptly can be used as the former support of viscose, substrate and basement membrane epithelial growth, healthy are provided, can be used for ocular surface reconstruction.
4. when changing the refractive status of eye, can make it have different refractive diopters, be used for the treatment of ametropia by operation methods such as corneal lens arts by changing the thickness of substrate.
In addition, the cell-eliminating coanea matrix that the present invention relates to is by reaching the in vitro toxicity test, stimulating coherent detections such as sensitization test (STT) and immunology in the body, meet the biological assessment standard, confirm that material is safe and reliable to receptor, can not constitute harm biology receptor part and whole body.
The specific embodiment
A kind of cell-eliminating coanea matrix of the present invention and preparation method thereof is further set forth by following examples.
Embodiment 1
A kind of cell-eliminating coanea matrix, mainly the collagen fiber flaggy that is parallel to each other by hundreds of (about 100-250) constitutes, and has cell to be sloughed the space that the back forms between the collagen fiber flaggy.The collagen fiber flaggy is parallel to each other, and is big or small consistent, equates at interval, also contains corneal stroma (comprising sticking polyprotein and glycoprotein etc.) in the space between the collagen fiber flaggy and takes off residual after birth and the endochylema composition in cell processing back.Described cell-eliminating coanea matrix regular shape, its thickness are (concrete shape and specification are decided on the situation of clinical case) about 0.1-0.5mm.
The preparation method of cell-eliminating coanea matrix, taking the heterogenous animal corneal stroma is raw material, and going cell to handle through the chemosmosis method can obtain, and step is as follows:
The chemosmosis method is made and to be taken off cell based cornea matter: with concentration is that 0.1% anion surfactant SDS solution was handled hetero stroma of cornea 7 hours under 37 ℃ condition, make the capable histopathology of paraffin section and observe, the result shows that substrate inner cell composition can be sloughed.
The cell based cornea matter of taking off of above-mentioned preparation is used for following test:
1. the described cell based cornea matter of taking off is used for the lamellar cornea transplanting
The damaged patient of the corneal opacity, ulcer or corneal stroma, behind the cornea with trepan or cornea ablation cutter removal lesion portion, with suture or biogum the cell-eliminating coanea matrix of corresponding specification is planted sheet and be fixed in plant bed, the bag eye, after treating superficial epitheliumization, press the flaggy keratoplasty and handle.Postoperative can recover the transparency and the integrity thereof of cornea in various degree.Also can simultaneously this cell-eliminating coanea matrix be carried the not medicine or the cytokine of same-action by different carriers (as liposome, Emulsion, millimicro ball etc.), as the VEGF of anti-new vessels, promote adherent ICAM-1 of epithelium and antibiotic etc., move to improve the flaggy angle that the postoperative new vessels forms, epithelial growth slowly and complication such as secondary infection.
2. the described cell based cornea matter of taking off is used for operation on pterygium
Routinely behind the pterygium resection operation regulation excision pterygium, the cleaning wound surface, getting the cell-eliminating coanea matrix that contains limbus girdle part sclera (circumferential edges is poor) that matches with damaged limbus of corneae fixes with suture or biogum, the conjunctiva that tractive cuts, cover the sclera part of cell-eliminating coanea matrix, pressure dressing art eye.Press pterygium associating amnion transplantation operation back conventional treatment.
3. take off cell based cornea matter to be used to repair sclera damaged described
Behind cleaning corneosclera staphyloma patient's the pathological changes surface, will fix with suture or biogum, sew up conjunctiva, pressure dressing art eye with the cell stroma of sclera (circumferential edges is poor) that takes off that damaged sclera matches.
Embodiment 2
A kind of cell-eliminating coanea matrix is made of hundreds of the collagen fiber flaggies that are parallel to each other, and has cell to be sloughed the space that the back forms between the collagen fiber flaggy.The collagen fiber flaggy is parallel to each other, and size is consistent, equates at interval, and its basis comprises collagen fiber, corneal stroma (comprising sticking polyprotein and glycoprotein etc.) and takes off cell and handle back residual after birth and endochylema composition.Described cell-eliminating coanea matrix is the circle of diameter range about 1-10mm, and its thickness is about 0.3mm.(concrete diameter and thickness can be moulding according to refractive status and the pathological changes situation of suffering from eye)
The preparation method of cell-eliminating coanea matrix, taking the allogeneic corneal stroma is raw material, and this cornea comprises the CSC that attaches on cornea, and going cell to handle through the chemosmosis method can obtain, and step is as follows:
The chemosmosis method is made and to be taken off cell based cornea matter: with concentration is that 1% detergent TritonX-100 solution was handled hetero stroma of cornea 24 hours under 37 ℃ condition, make the capable histopathology of paraffin section and observe, the result shows that substrate inner cell composition can be sloughed.
The cell based cornea matter of taking off of above-mentioned preparation is used for following test:
1. the described cell based cornea matter of taking off is used for the corneal lens art
By the requirement of anterior corneal surface eyeglass art, manufacture embedding groove at anterior corneal surface, will have in the poor circumferential edges insertion groove of suitable dioptric cell-eliminating coanea matrix sheet, fix pressure dressing art eye with suture or biogum.Press corneal lens art operation back conventional treatment.The diopter of postoperative patient can have improvement in various degree.
2. the described cell based cornea matter of taking off is used for implantation between corneal stroma
Be used for correction of refractive errors and treatment keratoconus.Select to have suitable dioptric cell-eliminating coanea matrix sheet according to patient's diopter.Do lamellar cornea or corneal stroma interlayer capsule bag, the cell-eliminating coanea matrix sheet of selecting is implanted in the inter-lamination of cornea, after fixing, pressure dressing art eye.Press corneal lens art operation back conventional treatment.
3. the described cell based cornea matter of taking off is used to construct engineered biological cornea:
When cornea histoengineering makes up, at the front surface plantation epithelial cell of cell-eliminating coanea matrix, rear surface plantation endotheliocyte, the 3-D solid structure of formation cornea.
Claims (9)
1. a cell-eliminating coanea matrix is characterized in that: mainly be made of hundreds of the fibrolamellars that are parallel to each other, have cell to be sloughed the space that the back forms between the fibrolamellar.
2. cell-eliminating coanea matrix according to claim 1 is characterized in that: mainly comprise collagen fiber, corneal stroma and take off residual after birth and the endochylema composition in cell processing back.
3. cell-eliminating coanea matrix according to claim 1 is characterized in that: the thickness of described cell-eliminating coanea matrix is 0.01～5.0mm.
4. cell-eliminating coanea matrix according to claim 1 is characterized in that: described cell-eliminating coanea matrix is that diameter is the circle of 1mm～40mm.
5. according to the arbitrary described cell-eliminating coanea matrix of claim 1-4, it is characterized in that: carry medicine and/or cytokine in the described cell-eliminating coanea matrix.
6. method for preparing the described cell-eliminating coanea matrix of claim 1 is characterized in that: adopting heterogenous animal class or allogeneic corneal stroma is raw material, handles through the past cell, obtains described cell-eliminating coanea matrix.
7. the method for preparing the described cell-eliminating coanea matrix of claim 1 according to claim 6, it is characterized in that: go cell to be treated to the chemosmosis method, step is as follows: with concentration is that 0.05～5% anion surfactant SDS solution was handled hetero stroma of cornea 4～24 hours under 4～50 ℃ condition.
8. the method for preparing the described cell-eliminating coanea matrix of claim 1 according to claim 6, it is characterized in that: go cell to be treated to the chemosmosis method, step is as follows: with concentration is that 0.01～5% detergent TritonX-100 solution was handled hetero stroma of cornea 24～48 hours under 4～50 ℃ condition, again with trypsinization 2-12 hour.
9. method for preparing the described cell-eliminating coanea matrix of claim 1 is characterized in that: heterogenous animal class or allogeneic corneal stroma be independent cornea, the cornea that has sclera, have the cornea of conjunctiva or have the cornea of CSC.
Priority Applications (2)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN200710027698A CN101066471B (en) | 2007-04-25 | 2007-04-25 | Cell-eliminating coanea matrix and its preparation process |
| PCT/CN2008/000661 WO2008131639A1 (en) | 2007-04-25 | 2008-04-02 | A cell-removing cornea substrate and a method of preparation thereof |
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| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN200710027698A CN101066471B (en) | 2007-04-25 | 2007-04-25 | Cell-eliminating coanea matrix and its preparation process |
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| CN101066471A true CN101066471A (en) | 2007-11-07 |
| CN101066471B CN101066471B (en) | 2010-05-19 |
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| CN200710027698A Expired - Fee Related CN101066471B (en) | 2007-04-25 | 2007-04-25 | Cell-eliminating coanea matrix and its preparation process |
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| WO (1) | WO2008131639A1 (en) |
Cited By (13)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| WO2008131639A1 (en) * | 2007-04-25 | 2008-11-06 | Zhongshan Ophthalmic Center, Sun Yat-Sen University | A cell-removing cornea substrate and a method of preparation thereof |
| WO2009117858A1 (en) * | 2008-03-24 | 2009-10-01 | 中山大学中山眼科中心 | A method for preparing the decellularized matrix |
| CN101433478B (en) * | 2007-11-14 | 2012-05-23 | 上海交通大学医学院附属第九人民医院 | Whole layer biological cornea as well as construction method and use thereof |
| CN102599991A (en) * | 2012-03-20 | 2012-07-25 | 中山大学中山眼科中心 | Application of beautifying cornea lens |
| CN102793593A (en) * | 2012-09-03 | 2012-11-28 | 于好勇 | Heterogeneous scleral piece used for posterior scleral reinforcement and preparation method thereof |
| CN103127549A (en) * | 2013-02-01 | 2013-06-05 | 中山大学中山眼科中心 | Decellularized eyelid board and preparation method thereof |
| CN101917932B (en) * | 2009-03-04 | 2013-08-21 | 首尔大学校产学协力团 | Method for processing porcine cornea for decellularization |
| CN103892938A (en) * | 2014-04-01 | 2014-07-02 | 薛春燕 | Corneal lamellar piece and production method thereof |
| CN105288736A (en) * | 2015-11-24 | 2016-02-03 | 北京清源伟业生物组织工程科技有限公司 | Preparation method of acellular dermal matrix cornea and ocular surface tissue restoration material |
| CN107106291A (en) * | 2014-12-29 | 2017-08-29 | 艾扬医药有限公司 | Artificial cornea |
| CN107233143A (en) * | 2017-05-31 | 2017-10-10 | 广州新诚生物科技有限公司 | Remove cell corneal stroma lens and preparation method thereof |
| CN108852615A (en) * | 2018-05-09 | 2018-11-23 | 山东省眼科医院 | A kind of plant piece and corneal stroma pouch for corneal stroma implantation |
| CN110841112A (en) * | 2011-09-02 | 2020-02-28 | Adeka株式会社 | Method for producing decellularized tissue product, and graft having decellularized tissue product |
Family Cites Families (6)
| Publication number | Priority date | Publication date | Assignee | Title |
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| US20060153928A1 (en) * | 2003-02-26 | 2006-07-13 | Shigeru Kinoshita | Aminion-origin medical material and method of preparing the same |
| CN100368534C (en) * | 2003-05-01 | 2008-02-13 | 四川大学华西医院 | Bioderived amnion, composite bioderived amnion and its preparation method |
| CN1326502C (en) * | 2003-08-07 | 2007-07-18 | 中山大学中山眼科中心 | Artificial tissue engineeing biological cornea |
| CN100333702C (en) * | 2004-04-28 | 2007-08-29 | 浙江大学医学院附属邵逸夫医院 | Exogenous cornea substrate without cells and its preparation method and use |
| JP4463124B2 (en) * | 2005-01-27 | 2010-05-12 | 史郎 天野 | Method for reducing the amount of cells contained in living tissue and acellularized living tissue |
| CN101066471B (en) * | 2007-04-25 | 2010-05-19 | 中山大学中山眼科中心 | Cell-eliminating coanea matrix and its preparation process |
-
2007
- 2007-04-25 CN CN200710027698A patent/CN101066471B/en not_active Expired - Fee Related
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2008
- 2008-04-02 WO PCT/CN2008/000661 patent/WO2008131639A1/en active Application Filing
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| Publication number | Priority date | Publication date | Assignee | Title |
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| WO2008131639A1 (en) * | 2007-04-25 | 2008-11-06 | Zhongshan Ophthalmic Center, Sun Yat-Sen University | A cell-removing cornea substrate and a method of preparation thereof |
| CN101433478B (en) * | 2007-11-14 | 2012-05-23 | 上海交通大学医学院附属第九人民医院 | Whole layer biological cornea as well as construction method and use thereof |
| WO2009117858A1 (en) * | 2008-03-24 | 2009-10-01 | 中山大学中山眼科中心 | A method for preparing the decellularized matrix |
| CN101274106B (en) * | 2008-03-24 | 2011-11-09 | 中山大学中山眼科中心 | Method for preparing acellular matrix |
| CN101917932B (en) * | 2009-03-04 | 2013-08-21 | 首尔大学校产学协力团 | Method for processing porcine cornea for decellularization |
| CN110841112A (en) * | 2011-09-02 | 2020-02-28 | Adeka株式会社 | Method for producing decellularized tissue product, and graft having decellularized tissue product |
| CN102599991A (en) * | 2012-03-20 | 2012-07-25 | 中山大学中山眼科中心 | Application of beautifying cornea lens |
| CN102793593A (en) * | 2012-09-03 | 2012-11-28 | 于好勇 | Heterogeneous scleral piece used for posterior scleral reinforcement and preparation method thereof |
| CN102793593B (en) * | 2012-09-03 | 2015-06-10 | 于好勇 | Heterogeneous scleral piece used for posterior scleral reinforcement and preparation method thereof |
| CN103127549A (en) * | 2013-02-01 | 2013-06-05 | 中山大学中山眼科中心 | Decellularized eyelid board and preparation method thereof |
| CN103892938A (en) * | 2014-04-01 | 2014-07-02 | 薛春燕 | Corneal lamellar piece and production method thereof |
| CN107106291A (en) * | 2014-12-29 | 2017-08-29 | 艾扬医药有限公司 | Artificial cornea |
| CN105288736A (en) * | 2015-11-24 | 2016-02-03 | 北京清源伟业生物组织工程科技有限公司 | Preparation method of acellular dermal matrix cornea and ocular surface tissue restoration material |
| CN107233143A (en) * | 2017-05-31 | 2017-10-10 | 广州新诚生物科技有限公司 | Remove cell corneal stroma lens and preparation method thereof |
| WO2018219045A1 (en) * | 2017-05-31 | 2018-12-06 | 广州新诚生物科技有限公司 | Decellularized corneal stromal lens and preparation method therefor |
| CN108852615A (en) * | 2018-05-09 | 2018-11-23 | 山东省眼科医院 | A kind of plant piece and corneal stroma pouch for corneal stroma implantation |
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| Publication number | Publication date |
|---|---|
| CN101066471B (en) | 2010-05-19 |
| WO2008131639A1 (en) | 2008-11-06 |
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