CN101051049A - Card type mycoplasma pneumiae anti-body detection reagent box - Google Patents
Card type mycoplasma pneumiae anti-body detection reagent box Download PDFInfo
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- CN101051049A CN101051049A CN 200710015485 CN200710015485A CN101051049A CN 101051049 A CN101051049 A CN 101051049A CN 200710015485 CN200710015485 CN 200710015485 CN 200710015485 A CN200710015485 A CN 200710015485A CN 101051049 A CN101051049 A CN 101051049A
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Abstract
A card type of antibody kit for detecting mycoplasma pneumoniae consists of detection plate includes plate body, plate cover and box shaped body. It is featured as firm-jointing plate cover with plate body, arranging detection hole on plate cover, setting reaction film and a gold labeling bottom as well as cleaning mixture bottle at position in plate body corresponding to detection hole.
Description
Technical field
The present invention relates to a kind of kit, specifically, relate to a kind of card type mycoplasma pneumiae anti-body detection reagent box.
Background technology
Mycoplasma pneumoniae belongs to a kind of special microorganism, and oral cavity, the parotid gland that can colonize in the people etc. located.Mycoplasma pneumoniae is neither virus, neither bacterium, but between virus and bacterium a kind of microorganism between the two, it mainly causes school-ager and teenager's lower respiratory infection, can pass through the respiratory tract droplet transmission.Approximately will take place once popular every 4～5 years Eaton agent pneumonias, based on the winter-spring season morbidity, the very difficult difference of respiratory tract infection that the clinical symptoms of mycoplasma pneumoniae infection possesses diversity and causes with other reason, mycoplasma pneumoniae can make tracheae, bronchus and bronchiole epithelial hyperplasia.Infect the back and some non-special symptoms just occur after 10～20 days,, between infection period, also may develop into interstitial pneumonia as fever, headache, non-productive cough etc.
Mycoplasma pneumoniae is one of important cause of disease of human ARI, can cause SARS (Severe Acute Respiratory Syndrome), tracheitis, bronchitis etc., there are about 10% adult's pneumonia and 20%～25% children Streptococcus to cause by mycoplasma pneumoniae, can also cause the outer complication of lung of other system, as meningitis, brain stem inflammation, myocarditis, pericarditis, ephritis etc.Because mycoplasma pneumoniae is to general medicine resistance, so but in time make a definite diagnosis specific treatment, the shortening course of treatment, minimizing complication and poor prognosis generation.Diagnostic method commonly used at present has metabolic inhibition test, complement fixation test (CFT) and euzymelinked immunosorbent assay (ELISA) etc., metabolic inhibition test, complement fixation test (CFT) and euzymelinked immunosorbent assay (ELISA) complex operation, need some large-scale instrument and equipments, need professional's operation experiments, use inconvenient.
Summary of the invention
The purpose of this invention is to provide a kind of high specificity, highly sensitive, efficient and convenient, safety, result accurately, adapt to the card type mycoplasma pneumiae anti-body detection reagent box of clinical needs.
For achieving the above object, the technical solution used in the present invention is: card type mycoplasma pneumiae anti-body detection reagent box is characterized in that: described kit comprises:
Check-out console, described check-out console is the box-like body that comprises plate body, plate lid, described plate lid is fixedlyed connected with plate body, described plate covers to be provided with and detects the hole, be provided with reaction film with detection corresponding position, hole site in the described plate body, one end of described reaction film is provided with bag by MP detection of antigens district, and the other end is provided with bag by the check plot of anti-mouse antibody, and the bottom of described reaction film is provided with adsorptive pads;
One gold medal mark liquid bottle, described gold is marked the mark bond that fills collaurum nano particle and monoclonal antibody in the liquid bottle; With
One Washing liquid bottle fills cleansing solution, in order to soak into diaphragm, to wash background and go nonspecific reaction.
As the prioritization scheme of technique scheme, described reaction film is a nitrocellulose filter.
As the another kind of prioritization scheme of technique scheme, the bottom surface of described plate lid is provided with several register pins that is used for fixing plate lid and plate body, and described plate body is provided with the locating slot that cooperates with register pin.
As another prioritization scheme of technique scheme, the position mark that described plate covers corresponding detection zone has letter " T ", and the position mark of corresponding check plot has letter " C ".
As the further prioritization scheme of technique scheme, described kit comprises:
One external packing box fills 40 check-out consoles with packaging of aluminium foil bag, a gold mark liquid bottle, a Washing liquid bottle in the described external packing box.
The using method of described kit may further comprise the steps:
(1) sample collection
Take venous blood in totally not adding in the container of anti-coagulants, leave standstill and make blood clotting, separation of serum is prepared to detect;
(2) detect
A, taking-up check-out console, equilibrium at room temperature 20～30 minutes;
B, drip 1 of cleansing solution in detecting the hole, treat that liquid is moistening fully with nitrocellulose filter;
C, add test serum 150ul in detecting the hole, treat that liquid fully sucks;
D, drip 3 of gold mark liquid in detecting the hole, treat that liquid fully sucks;
E, drip 3 of cleansing solutions in detecting the hole, treat that liquid fully sucks back observations in 3 minutes.
(3) result judges
After splashing into liquid in the detection hole of check-out console, absorbent material makes liquid slowly descend diafiltration, finishes specific antigen-antibody reaction in diafiltration.
A, feminine gender:
If do not have mycoplasma pneumoniae antibody in the test sample, the gold mark monoclonal antibody in the reaction system can't form indirect antigen-antibody reaction with the MP antigen of bag quilt, i.e. redfree reaction marking appearance in mark has the detection zone of letter " T ".Simultaneously, the how anti-reaction bonded of anti-mouse IgG of gold mark monoclonal antibody in the reaction system and check plot bag quilt, have the place, check plot of letter " C " to form a red deposition reaction mark at mark, this shows does not have mycoplasma pneumoniae antibody in the sample, negative result; Illustrate that simultaneously reagent W-response system works is normal.
B, the positive:
If have mycoplasma pneumoniae antibody in the test sample, the then MP antigen generation antigen-antibody reaction of bag quilt on its elder generation and the nitrocellulose filter, form antigen-antibody complex and be trapped on the film, other irrelevant antibody and material are then filtered, when adding the anti-human monoclonal antibodies of gold mark again, just react with it, form the immune complex of gold mark anti-human monoclonal antibodies-MP antibody-MP antigen, promptly in mark has the detection zone of letter " T ", have red reaction marking to occur.Simultaneously, the how anti-reaction bonded of anti-mouse IgG of gold mark monoclonal antibody in the reaction system and check plot bag quilt has the place, check plot of letter " C " to form a red deposition reaction mark at mark.Detection zone has certification mark to occur, and this shows mycoplasma pneumoniae antibody in the sample, positive result.
C, invalid:
After the reaction, there is the place, check plot of letter " C " not develop the color, shows that this detection is invalid at mark.
(4) points for attention
A, the necessary fresh serum that uses are measured.Minority sample such as hyperlipemia, haemolysis etc. can make background dark partially, generally do not influence the result and judge.
B, adding must not be if having time at interval between each step operation of liquid; Add in the same step and wait its infiltration after liquid should once add rapidly.
The result of c, the appearance that exceeds schedule time is invalid.
The power of d, check plot mark is not represented the quality of reagent quality, as long as its colour developing is clearly visible, shows that promptly reagent is effective.
E, different product, forbid to use with the mark of the gold between product different batches liquid.
(5) store and transportation
A, kit are put 2～8 ℃ of refrigerations, and the term of validity is 10 months.
But b, the transportation of kit short-term normal temperature.Severe winter or season hot summer should be taked corresponding safeguard procedures, avoid freeze thawing or long term high temperature.
Following table is the clinical assays experimental data of kit of the present invention:
Beneficial effect: the present invention adopts above technical scheme, has high specificity, highly sensitive, quick, easy, directly perceived, does not need special instrument and equipment, adapts to the advantage of clinical needs, and therefore, the present invention has higher social value and economic worth.
The invention will be further described below in conjunction with drawings and Examples.
Description of drawings
Accompanying drawing 1 is the structural representation of the embodiment of the invention 1;
Accompanying drawing 2 be in the accompanying drawing 1 A-A to structural representation;
Accompanying drawing 3 is the structural representation of the embodiment of the invention 2;
Accompanying drawing 4 be in the accompanying drawing 3 B-B to structural representation.
Embodiment
The using method of kit of the present invention may further comprise the steps:
(1) sample collection: take venous blood in totally not adding in the container of anti-coagulants, leave standstill and make blood clotting, separation of serum is prepared to detect;
(2) detect:
A, taking-up check-out console 1, equilibrium at room temperature 20～30 minutes;
B, drip 1 of cleansing solution in square detection hole 2, treat that liquid is moistening fully with nitrocellulose filter;
C, add test serum 150ul in square detection hole 2, treat that liquid fully sucks;
D, drip 3 of gold mark liquid in square detection hole 2, treat that liquid fully sucks;
E, drip 3 of cleansing solutions in square detection hole 2, treat that liquid fully sucks the back and saw and hold a memorial ceremony for the result in 3 minutes.
(3) result judges: after splashing into liquid in the detection hole 2 of check-out console, absorbent material makes liquid slowly descend diafiltration, finishes specific antigen-antibody reaction in diafiltration.
A, feminine gender:
After the reaction, have 6 places, check plot of letter " C " to form a red deposition reaction line at mark, have the reactionless line in detection zone 5 places of letter " T " to occur at mark, showing does not have mycoplasma pneumoniae antibody, negative result in the sample;
B, the positive:
After the reaction, have 6 places, check plot of letter " C " to form a red deposition reaction line at mark, have detection zone 5 places of letter " T " to form a red deposition reaction line at mark simultaneously, showing has mycoplasma pneumoniae antibody, positive result in the sample;
C, invalid:
After the reaction, there are 6 places, check plot of letter " C " not develop the color, show that this detection is invalid at mark.
Claims (5)
1, card type mycoplasma pneumiae anti-body detection reagent box is characterized in that: described kit comprises:
Check-out console (1), described check-out console (1) is the box-like body that comprises plate body (12), plate lid (11), described plate lid (11) is fixedlyed connected with plate body (12), described plate lid (11) is provided with and detects hole (2), be provided with reaction film (3) with detection corresponding position, position, hole (2) in the described plate body (12), one end of described reaction film (3) is provided with bag by MP detection of antigens district (5), and the other end is provided with bag by the check plot of anti-mouse antibody (6), and the bottom of described reaction film (3) is provided with adsorptive pads (4);
One gold medal mark liquid bottle, described gold is marked the mark bond that fills collaurum nano particle and monoclonal antibody in the liquid bottle; With
One Washing liquid bottle fills cleansing solution, in order to soak into diaphragm, to wash background and go nonspecific reaction.
2, card type mycoplasma pneumiae anti-body detection reagent box as claimed in claim 1 is characterized in that: described reaction film (3) is a nitrocellulose filter.
3, card type mycoplasma pneumiae anti-body detection reagent box as claimed in claim 2, it is characterized in that: the bottom surface of described plate lid (11) is provided with several register pins (7) that is used for fixing plate lid (11) and plate body (12), and described plate body (12) is provided with the locating slot (8) that cooperates with register pin (7).
4, card type mycoplasma pneumiae anti-body detection reagent box as claimed in claim 3 is characterized in that: the position mark that described plate lid (11) is gone up corresponding detection zone (5) has letter " T ", and the position mark of corresponding check plot (6) has letter " C ".
5, as claim 1,2,3,4 one of them described card type mycoplasma pneumiae anti-body detection reagent box, it is characterized in that: described kit comprises:
One external packing box fills 40 check-out consoles with packaging of aluminium foil bag (1), a gold mark liquid bottle, a Washing liquid bottle in the described external packing box.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN 200710015485 CN101051049A (en) | 2007-05-11 | 2007-05-11 | Card type mycoplasma pneumiae anti-body detection reagent box |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN 200710015485 CN101051049A (en) | 2007-05-11 | 2007-05-11 | Card type mycoplasma pneumiae anti-body detection reagent box |
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| Publication Number | Publication Date |
|---|---|
| CN101051049A true CN101051049A (en) | 2007-10-10 |
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Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN 200710015485 Pending CN101051049A (en) | 2007-05-11 | 2007-05-11 | Card type mycoplasma pneumiae anti-body detection reagent box |
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| CN (1) | CN101051049A (en) |
Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102928589A (en) * | 2012-11-15 | 2013-02-13 | 南京凯基生物科技发展有限公司 | Colloidal gold method detection test strip and reagent kit for IgG antibody of respiratory disease and preparation method of reagent kit |
| CN101738475B (en) * | 2010-01-11 | 2013-04-17 | 杨致亭 | Hepatitis E virus antibody detection kit and preparation method thereof |
| CN108761073A (en) * | 2018-04-12 | 2018-11-06 | 潍坊市康华生物技术有限公司 | A kind of mycoplasma pneumoniae antigen detection kit and preparation method thereof |
-
2007
- 2007-05-11 CN CN 200710015485 patent/CN101051049A/en active Pending
Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101738475B (en) * | 2010-01-11 | 2013-04-17 | 杨致亭 | Hepatitis E virus antibody detection kit and preparation method thereof |
| CN102928589A (en) * | 2012-11-15 | 2013-02-13 | 南京凯基生物科技发展有限公司 | Colloidal gold method detection test strip and reagent kit for IgG antibody of respiratory disease and preparation method of reagent kit |
| CN108761073A (en) * | 2018-04-12 | 2018-11-06 | 潍坊市康华生物技术有限公司 | A kind of mycoplasma pneumoniae antigen detection kit and preparation method thereof |
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Application publication date: 20071010 |