CN101032564B - Health preserving wine for conditioning blood-fat and the preparing method - Google Patents
Health preserving wine for conditioning blood-fat and the preparing method Download PDFInfo
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- CN101032564B CN101032564B CN2007100568522A CN200710056852A CN101032564B CN 101032564 B CN101032564 B CN 101032564B CN 2007100568522 A CN2007100568522 A CN 2007100568522A CN 200710056852 A CN200710056852 A CN 200710056852A CN 101032564 B CN101032564 B CN 101032564B
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- resveratrol
- puerarin
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Abstract
The present invention discloses one kind of blood fat regulating health wine, which includes white liquor and puerarin, resveratrol, resveratrol glycoside and/or ursolic acid. Each 500 ml of the health wine contains at least 1 mg of the said health factors. The blood fat regulating health wine is mellow, stable and controllable in quality, and has the health function of preventing and treating atherosclerosis.
Description
Technical field
The present invention relates to food and protective foods, particularly relate to a kind of regulating blood fat health wine that contains health factor and preparation method thereof.
Background technology
Liquor historical of long standing and well established is that people entertain, one of important drink when celebrating.The ethanol content of liquor is higher, and other nutritive substance relative content is lower, and is little to the human body benefit after drinking.Along with the progress of society, the improving constantly of people's living standard, idea is also being brought in constant renewal in, and when requiring to drink, can not only enjoy drink happy, and will guarantee healthy.At present, the number of drinking white spirit is accustomed to for a long time up to more than one hundred million by China.In this colony, there have sizable ratio to exist blood fat to be higher, atherosclerotic danger.And this part philtrum hyperlipidemia belongs to the wet blood stasis category of motherland's medical science phlegm, major cause be on ordinary days eating and drinking without temperance, have a liking for food foie gras, impairing the spleen and stomach, the wet strong fortune of spleen and accumulatively wet raise phlegm, the wet resistance of phlegm network are due to rising in the hemostasis, the disease being located in the spleen stomach should be with invigorating the spleen for dissipating phlegm, and it is main that clearing damp is fallen turbid.How to make wine both keep the original style of liquor, have the antilipemic healthy trophism again.Therefore, in wine, dissolve in medicinal material with different health-care effecies or plurality of Chinese medicine materical crude slice and put into wine and soak and form, higher in order to prevention and blood fat reducing, atherosclerotic danger.This kind immersion process has been obtained certain effect, obtained the approval of partial mass, but also there is following shortcoming in this immersion process: quality product is wayward, and nutritive ingredient in the medicinal material and pharmaceutical component can not effectively be extracted, and the wine after soaking, color depth, bitter; Because coloured being difficult to when entertaining of wine enters dinner party wine, and mouthfeel is relatively poor, drink just as taking medicine, lost the aromatic of wine,, and added sugar often in order to solve sensory issues, still be difficult to be accepted, be unfavorable for alcohol user's health care to health when drinking by the people.
Summary of the invention
Technical problem to be solved by this invention is, overcomes the shortcoming of prior art, and a kind of crystalline powder that adds the activeconstituents of reducing blood-fat in wine is provided, and produces colourless clear saturating, pure taste, and it is higher to suppress blood fat, atherosclerotic health-care and nutritious wine.
The technical solution used in the present invention is: a kind of regulating blood fat health wine comprises liquor and plant milk extract; Described plant milk extract comprises puerarin, trans-resveratrol, polydatin, ursolic acid; Comprise in the described liquor plant milk extract one or more, contain from 1mg to liquor in every 500ml wine the plant milk extract of maximum saturation solubleness.
The proportioning of plant milk extract and liquor (w/v) is 1mg～400mg/500ml.
The liquor number of degrees are 0.5～65 degree.
Contain plant milk extract puerarin 1mg～398mg, trans-resveratrol and glycosides 1mg～300mg thereof, ursolic acid 1mg～200mg in the 500ml liquor.
Contain plant milk extract puerarin 30～300mg, trans-resveratrol and glycosides 20～300mg thereof, ursolic acid 5mg～100mg in the 500ml liquor.
Also comprise 1mg～300mg Fructus Crataegi extract.
Also comprise 1mg～300mg Fructus Crataegi extract.
A kind of preparation method of regulating blood fat health wine comprises the steps:
(1) plant is purified
Choose natural plant material: puerarin, trans-resveratrol, ursolic acid and hawthorn;
Raw material is carried out boiling, extraction, alcohol precipitation, concentrates the acquisition concentrated solution;
By macroporous resin purification, described macroporous resin purification comprises: upper prop, absorption and wash-out to concentrated solution;
Successively water and 10%～90% ethanol wash-out macroporous adsorbent resin is respectively collected elutriant, the dry crude product that obtains;
The purifying plant milk extract obtains effective constituent chemicals monomer reducing blood-fat plant milk extract white crystalline powder;
(2) but the finished product method of reducing blood-fat health wine
In the plant milk extract behind the above-mentioned E item gained purifying one or more are put into storage wine jar in proportion, take out with wine by pump repeatedly and fully merge, behind molecular sieve filtration, obtain reducing blood-fat health wine.
The invention has the beneficial effects as follows: regulating blood fat health bartender of the present invention has stayed the color and the taste of original liquor, and colourless or little yellow, clear saturating, free from extraneous odour have the sweet-smelling of wine, and health factor are clear and definite, stable and controllable for quality; The health factor that is added does not contain any chemical additive component, is a kind of green food and healthcare products of environment-friendly type, and it is higher that Chinese medicine content of active substance wherein has the blood fat of inhibition, atherosclerotic effect.Owing in wine, added new healthy element, make the colony of getting used to drinking for a long time, the health factor that can will have reducing blood lipid in daily drinking is sent in the body, reach and effectively prevent or control atherosclerotic formation, in the process of drinking, realize the health care, promote longevity.This health wine is clear thoroughly, sweet-smelling is strong can also have higher popularization and application values as dinner party wine.
Description of drawings
Fig. 1 is preparation method's schema of regulating blood fat health wine of the present invention.
Embodiment
Regulating blood fat health wine comprises liquor and plant milk extract; Described plant milk extract comprises puerarin, trans-resveratrol, ursolic acid; Comprise one or more mixture of plant milk extract in the described liquor, contain from 1mg to liquor in every 500ml wine the plant milk extract of maximum saturation solubleness.The proportioning of plant milk extract and liquor (w/v) is 1mg～400mg/500ml.Can contain health factor (active ingredient of Chinese herbs) puerarin 1mg～398mg, trans-resveratrol and glycosides 1mg～300mg, ursolic acid 1mg～200mg in the general 500ml liquor.The comparatively tangible mix proportion scheme of health-care effect also can adopt and contain health factor (active ingredient of Chinese herbs) puerarin 30～300mg, trans-resveratrol and glycosides 20～300mg, ursolic acid 40mg～200mg in 500ml liquor.The liquor number of degrees that regulating blood fat health wine is adopted can be 0.5～65 degree, and the preferred number of degrees are 38 degree.Regulating blood fat health wine can also comprise Fructus Crataegi extract 1mg～300mg.
Preparation and detection method to regulating blood fat health wine illustrated below:
(1) respectively to contain health factor of the present invention be the integration of drinking and medicinal herbs of plant milk extract or natural phant extraction, separation, purifying such as the macroporous adsorption resin technology that is used for protective foods and chromatogram purification technical point from, to prepare the effective constituent compound monomer with hepatoprotective effect that its purity reaches more than 90% be health factor.
(2), make new type health health wine and should meet following characteristics with a kind of health factor of the present invention or severally dissolve in the liquor by prescription: colourless or little yellow, limpid bright, free from extraneous odour, have the sweet-smelling of wine, health factor is clear and definite, stable and controllable for quality.
(3) adopt quantitatively the Quality Control Technology of measuring health factor content respectively of HPLC method accurately:
1. the HPLC method is measured the method for puerarin:
Chromatographic condition moving phase: methanol-water (25: 75);
Chromatographic column: C
18(5um), filler is kromasil;
Detect wavelength: 250nm;
The preparation of reference substance solution: it is an amount of that precision is measured the puerarin reference substance, makes the reference substance solution that every 1ml contains puerarin 40ug with 50% methyl alcohol.
Sample solution preparation: measure sample solution 5ml, place the 25ml measuring bottle, add 50% methyl alcohol and be diluted to scale, shake up, promptly.
Assay method: precision is measured reference substance solution and each 10～20ul of sample solution respectively, injects the HPLC instrument, measures, and calculates by external standard method, promptly.
2. the HPLC method is measured the method for trans-resveratrol and polydatin:
Chromatographic condition moving phase: acetonitrile-water (22: 78);
Chromatographic column: C
18(5um), filler is kromasil;
Detect wavelength: 306nm;
The preparation of reference substance solution: precision measures trans-resveratrol and the polydatin reference substance is an amount of, makes the reference substance solution that every 1ml contains trans-resveratrol and each 10ug of polydatin with 70% methyl alcohol.
Sample solution preparation: measure sample solution 5ml, place the 25ml measuring bottle, add 70% methyl alcohol and be diluted to scale, shake up, promptly.
Assay method: precision is measured reference substance solution and each 10～20ul of sample solution respectively, injects the HPLC instrument, measures, and calculates trans-resveratrol and polydatin content by external standard method, promptly
3. the HPLC method is measured the method for ursolic acid:
Chromatographic condition moving phase: methanol-water (25: 75);
Chromatographic column: C
18(5um), filler is kromasil;
Detect wavelength: 250nm;
The preparation of reference substance solution: it is an amount of that precision is measured the ursolic acid reference substance, makes the reference substance solution that every 1ml contains ursolic acid 40ug with 50% methyl alcohol.
Sample solution preparation: measure sample solution 5ml, place the 25ml measuring bottle, add 50% methyl alcohol and be diluted to scale, shake up, promptly.
Assay method: precision is measured reference substance solution and each 10～20ul of sample solution respectively, injects the HPLC instrument, measures, and calculates by external standard method, promptly.
Preparation method to concrete health factor is illustrated respectively below:
Select the plant material that mouth is light, look shallow of the integration of drinking and medicinal herbs of pure natural: puerarin, trans-resveratrol, polydatin and ursolic acid.
1) preparation of puerarin: the root of kudzu vine behind macroporous resin purification, is handled purifying, crystallization with the silica gel chromatography column chromatography through water extraction, promptly obtains white crystalline powder.
2) preparation of trans-resveratrol: when extracting trans-resveratrol, select 60%～80% ethanol to make extraction solvent, after extracting solution is concentrated into and is equivalent to the medicinal material charging capacity, add the less water dilution, diluent extracts with 8 times of volumes of acetic acid ethyl esters, extracting solution concentrate ethyl acetate extract, through the silica gel wash-out, collect elutriant, through decolouring, concentrate, freeze-drying promptly obtains white crystalline powder.
3) preparation of ursolic acid: when extracting ursolic acid, with 50%～80% extraction using alcohol, after extracting solution is concentrated into and does not have alcohol flavor (being equivalent to the medicinal material charging capacity), add small diluted amount, diluent is by macroporous resin column, and first water is eluted to colourless, uses 50%～80% ethanol elution again, collect elutriant, through decolouring, concentrate, freeze-drying promptly obtains white crystalline powder.
4) preparation of polydatin: when extracting polydatin, select 20%～50% ethanol to make extraction solvent, after extracting solution is concentrated into and is equivalent to the medicinal material charging capacity, add the less water dilution, diluent is centrifugal, removes not tolerant, supernatant liquor is crossed macroporous adsorbent resin, wash with water to closely colourless, use 20%～50% wash-out again, collect elutriant, through decolouring, being concentrated into does not have the alcohol flavor, and again with 10 times of volumes of acetic acid ethyl ester gradation extractions, extracting solution is concentrated separates out crystallization and promptly obtain the white crystalline material.
5) with a kind of above-mentioned white crystalline powder or severally dissolve in the 500ml liquor by prescription, make new type health health wine, adopt quantitative HPLC method to measure health factor content.
The quality of medicinal material standard that the present invention uses all meets " the Chinese people are state's officinal relevant regulations altogether ", and the physico-chemical property and the pharmacological effect of used medicinal material are described below in the reducing blood-fat health wine:
The root of kudzu vine is the integration of drinking and medicinal herbs article, and puerarin is a main effective constituent in the root of kudzu vine, and its molecular formula is 4, and 7-dihydroxyl-8-8-D-glucose isoflavones is white needle, water-soluble and ethanol.The experiment report is arranged, puerarin can effectively reduce the value of high lipid diet rat blood serum TG, TC, LDL-C level and AI, puerarin intervention group rat blood serum HDL-C significantly raises, and normal feed group rat plasma HDL-C obviously reduces, and will play better action to the prevention hyperlipidemia.Main component is pycnogenols, trans-resveratrol and polydatin in Semen Vitis viniferae and the Pericarpium Vitis viniferae.Trans-resveratrol and polydatin are white crystals, and certain solubleness is arranged in Diluted Alcohol; Pycnogenols is a kind of total aldehydes matter, and sorrel can be dissolved in Diluted Alcohol.They are the high-efficiency antioxidant agent, can remove free radical effectively, the peroxide injury that Green Tea Extract is caused and playing a protective role.
Trans-resveratrol is a kind of polyphenolic compound, and TC, TG, LDL-C and the Al of hyperlipidemia rats are obviously reduced, rising HDL-C and HDL-C/TC ratio, and certain dose-effect relationship is arranged.This may be because trans-resveratrol has the phenol hydroxyl, easily pass through autoxidation, suppress the oxidation of cholesterol and unsaturated fatty acids, prevent the accumulation of cholesterol and other enol classes and neutral fat in the blood, platelet aggregation-against, improve the blood peripheral circulation, thereby play preventing hyperlipidemia, suppress arteriosclerosis and form.
But the Fructus Crataegi total flavones reducing cholesterol suppresses hyperlipemia animal TC, TG and improves the SOD vigor, reduces the generation of free radical, obviously reduces all lipid parameters, and hyperlipidaemia is had significant prevention and therapeutic action.The uvaol extract, obvious to the triglyceride reducing effect especially.Ursolic acid is the main component of the contained triterpene substance of hawthorn, and it is the crystalline powder of white, but poorly water-soluble; Quercetin 3-galactoside is the important component of Fructus Crataegi flavone, and it is faint yellow needle crystal, is soluble in ethanol.
Puerarin, trans-resveratrol, ursolic acid etc. have cholesterol in the blood fat reducing, triglyceride level, improve the SOD vigor, reduce the generation of free radical, obviously reduce all lipid parameters, and hyperlipidaemia is had significant prevention and therapeutic action.
There are some researches prove, hyperlipemia, blood lipid level descends 1%, the mortality ratio of cardiovascular and cerebrovascular diseases can descend 2%, therefore, the blood fat reducing level is prevention and treatment cardiovascular and cerebrovascular diseases important and effective means are one of current social urgent problems.Therefore, in wine, add reducing blood-fat health factors such as a certain amount of puerarin, salvianolic acid, ursolic acid, trans-resveratrol, make it organically to combine with liquor, the health factor that utilizes this carrier of wine will have reducing blood lipid is sent in the body of being accustomed to the crowd that drinks, reaches the health care purpose of reducing blood-fat.
Drinking method: each cuvette, noon and evening respectively once also can increase the amount of drinking according to the capacity for liquor of oneself slightly, are as the criterion up to taking effect, and adhere to drinking resistibility and healthy being beneficial to health.
Actual effect of the present invention mainly is to taste experiment by people, and the real reaction effect of prolonged application, similar compare is verified.On probation through people more than 100, effect is obvious, and spirit is happy after drinking, and feels that generally health is comfortable.Contain cholesterol, triglyceride level health factor in the blood fat reducing of various compositions simultaneously, show through acute toxicity test and long term toxicity test: the health factor among the present invention comprises that one or more and the liquor compatibility in puerarin, trans-resveratrol, polydatin, the ursolic acid is without any side effects, safe and reliable.In different crowds, try out and obtained the extraordinary effect of drinking.
Embodiment 1
The preparation of health factor: highly purified puerarin, trans-resveratrol, polydatin, ursolic acid composition.
1. the preparation of puerarin: root of kudzu vine 500g is with 14 times of water extraction 2 times, each 2 hours, extracting solution concentrating under reduced pressure, being concentrated into relative density is 1.10 (50 ℃), concentrated solution adds 2 times of water dilutions, and HPD100 type macroporous resin on the diluent washes with water to colourless, use 50% ethanol elution instead, 4 times of column volumes of wash-out are collected elutriant, concentrate, enriched material obtains white crystalline powder behind silica decoloration, purifying.
2. the preparation of trans-resveratrol: get Pericarpium Vitis viniferae 5kg, select 70% ethanol to make extraction solvent, after extracting solution is concentrated into and is equivalent to the medicinal material charging capacity, add small diluted amount, diluent extracts with 10 times of volumes of acetic acid ethyl esters, extracting solution concentrate ethyl acetate extract, through the silica gel wash-out, collect elutriant, through decolouring, concentrate, freeze-drying promptly obtains white crystalline powder.
3. the preparation of ursolic acid: get hawthorn 3kg, use 70% extraction using alcohol, after extracting solution is concentrated into and does not have alcohol flavor (being equivalent to the medicinal material charging capacity), add small diluted amount, diluent is by HPD300 type macroporous resin column, and first water is eluted to colourless, uses 70% ethanol elution again, collect elutriant, through silica decoloration, concentrate, freeze-drying promptly obtains white crystalline powder.
4. polydatin preparation: get Pericarpium Vitis viniferae 2kg, make the extraction solvent, extract No. 2 times extracting solution with 50% ethanol, be evaporated to 2000ml, add 1.0 times of water dilutions, cross the YWD04 resin, be washed to closely colourless, use 25% ethanol elution, collect elutriant, be evaporated to 1000ml, with ethyl acetate 4000ml gradation extraction, extraction liquid concentrates and separates out crystallization, promptly.
Need to prove especially; the extract and the effective constituent of a lot of medicinal materials all have commercially available product; therefore active ingredient of Chinese herbs of the present invention also can be replaced with corresponding commercially available extract; the proportioning of each component extract is to be converted to the proportioning that is equivalent to described active ingredient of Chinese herbs according to index components content, and these all belong to protection scope of the present invention.
Among the preparation method of regulating blood fat health wine, the number of degrees of liquor can not exert an influence to the injected volume of health factor among the described embodiment of following table.
Take out fusion with the wine pump in the health factor input storage wine jar with above different proportionings, colourless through promptly can be made into behind the screen, clear saturating, free from extraneous odour have the sweet-smelling of wine, but health factor is clear and definite, health care's wine of stay-in-grade reducing blood-fat.
It is worthy of note; protection scope of the present invention is not limited to above-mentioned specific examples mode; health factor can also be put into wine such as red wine, beer and yellow rice wine; all can realize identical effect; according to basic fundamental design of the present invention; those of ordinary skills need not through creative work, and the embodiment that can associate all belongs to protection scope of the present invention.
Claims (5)
1. a regulating blood fat health wine is characterized in that: be made up of liquor and plant milk extract; Plant milk extract in the described liquor is puerarin, trans-resveratrol, polydatin and ursolic acid; The plant milk extract of maximum saturation solubleness containing from 1mg to liquor in every 500ml wine.
2. a regulating blood fat health wine is characterized in that: be made up of liquor and plant milk extract; Plant milk extract in the described liquor is puerarin, trans-resveratrol, polydatin and ursolic acid; The proportioning w/v of plant milk extract and liquor is 1mg～400mg/500ml.
3. a regulating blood fat health wine is characterized in that: be made up of liquor and plant milk extract; Plant milk extract in the 500ml liquor is puerarin 1mg～398mg, trans-resveratrol and glycosides 1mg～300mg thereof and ursolic acid 1mg～200mg.
4. a regulating blood fat health wine is characterized in that: be made up of liquor and plant milk extract; Plant milk extract in the 500ml liquor is puerarin 30mg～300mg, trans-resveratrol and glycosides 20mg～300mg thereof and ursolic acid 5mg～100mg.
5. as claim 1 or 2 or 3 or 4 described regulating blood fat health wine, it is characterized in that: the liquor number of degrees are 0.5～65 degree.
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| Application Number | Priority Date | Filing Date | Title |
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| CN2007100568522A CN101032564B (en) | 2007-02-28 | 2007-02-28 | Health preserving wine for conditioning blood-fat and the preparing method |
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| CN2007100568522A CN101032564B (en) | 2007-02-28 | 2007-02-28 | Health preserving wine for conditioning blood-fat and the preparing method |
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| CN201010147507A Division CN101870944A (en) | 2007-02-28 | 2007-02-28 | Blood fat regulating healthcare wine and preparation method thereof |
| CN201010147500XA Division CN101940580A (en) | 2007-02-28 | 2007-02-28 | Health promotion wine for regulating blood fat and preparation method thereof |
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| CN101032564B true CN101032564B (en) | 2011-03-23 |
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Families Citing this family (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| AU2007101055A4 (en) * | 2007-10-31 | 2007-12-13 | Norrie, Philip Anthony Dr | Resveratrol Enhanced Wine |
| ITPI20110063A1 (en) * | 2011-06-08 | 2012-12-09 | Funcional Food Res S R L | ALCOHOLIC DRINK WITH HIGH CONTENT OF RESVERATROL AND RELATIVE PRODUCTION METHOD. |
| CN103548996A (en) * | 2013-10-27 | 2014-02-05 | 施东德 | Kumiss |
| AU2015256178B2 (en) | 2014-05-05 | 2020-02-27 | The Board Of Regents Of The University Of Texas System | Methods and compositions comprising ursolic acid and/or resveratrol for treating obesity, diabetes, or cancer |
| CN105368659A (en) * | 2015-11-12 | 2016-03-02 | 湖北省三鑫生物科技有限公司 | Fangxian county yellow rice wine containing resveratrol as well as preparation method and application thereof |
| CN112526045B (en) * | 2019-09-17 | 2023-03-31 | 云南中医药大学 | Method for simultaneously detecting or identifying effective components in heart-soothing and lipid-lowering tablets |
Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1472303A (en) * | 2003-07-11 | 2004-02-04 | 王德福 | Production of high content white veratrum alkaloids spirit |
| CN1560062A (en) * | 2004-03-11 | 2005-01-05 | 白栓锁 | Process of extracting puerariae and making food and medicine addied with it |
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Patent Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1472303A (en) * | 2003-07-11 | 2004-02-04 | 王德福 | Production of high content white veratrum alkaloids spirit |
| CN1560062A (en) * | 2004-03-11 | 2005-01-05 | 白栓锁 | Process of extracting puerariae and making food and medicine addied with it |
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