CN101011181A - Process for bacteria degradation of orange capsule clothes - Google Patents
Process for bacteria degradation of orange capsule clothes Download PDFInfo
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- CN101011181A CN101011181A CN 200710034429 CN200710034429A CN101011181A CN 101011181 A CN101011181 A CN 101011181A CN 200710034429 CN200710034429 CN 200710034429 CN 200710034429 A CN200710034429 A CN 200710034429A CN 101011181 A CN101011181 A CN 101011181A
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- spore
- orange
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Abstract
The invention relates to a method for using microbe to degrade the coat of orange, with high quality, efficiency and safety, without pollution and heavy metal left. The invention comprises that a, selecting the fungi or the fungi combination meet following conditions that the fungi can grow quickly and generate agamospores or sexual spores, and the generated external pectin has high activity on the degradation of enzyme, cellulose and hemi-cellulase, and the fungi is safe to the tin of orange; b, using fermentation to generate spores or mycelium from the fungi in step a; c, preparing the spores or mycelium into microbe degrading agent; d, removing skin of cleaned orange, splitting and removing the stems, adding the left orange into the microbe degrading agent solution, mixing to remove the coat.
Description
Technical field
The invention belongs to a kind of method that removes the capsule clothing, be specifically related to a kind of method that removes orange skin.
Background technology
China is world citrus industry big country, and cultivated area was 171.4 ten thousand hectares in 2005, occupied first place in the world, and output reaches 1601.95 ten thousand tons, occupies the second place of the world.Mandarin orange can is the leading products of mandarin orange processing, accounts for more than 80% of citrus processing capacity.Oranges and tangerines can 2005 annual export volumes reach 300,000 tons, account for more than 60% of volume of world trade, and export amount reaches 1.98 hundred million dollars, are China's citrus industry the most competitive products in the international market, become new " oranges and tangerines can kingdom " after Spain, Japan.
At present, Spain is submitting to European Union to implement at the mandarine can working out new technical standard to form TBT (Technical Barriers to Trade) after restrictive " Special Safeguard measure " do not become.China continues to use traditional acid-base method excystation clothing technology substantially in mandarin orange can processing, this explained hereafter efficient is low, and product quality is also very unstable.The acid-base method excystation clothing processing technology of tradition oranges and tangerines can has that acid base concentration is big more within the specific limits, and capsule clothing removal efficiency is high more, the hard brittleness of tangerine lobe is low more, petal-breaking rate is high and the high shortcoming of ton water consumption.And because the soda acid processing needs use water rinse, produce a large amount of acidic and alkaline waste waters, simultaneously, residues of harmful substances such as heavy metal has reduced the quality and safety of oranges and tangerines canned packs in the soda acid, meet with technology barriers easily, and the problem of environmental pollution that brings also meets with green barrier easily, and these are with the superiority of serious threat China oranges and tangerines can industry in the international market.And, because current technology industrial water amount is big, reach the 60-80 ton, bring heavier cost burden to enterprise, restricted the development of enterprise.
Summary of the invention
The inventive method technical problem to be solved is to overcome above-mentioned the deficiencies in the prior art, provide a kind of production efficiency height, constant product quality, safe, free from environmental pollution, do not have heavy-metal residual and utilize the method for bacteria degradation of orange capsule clothes.
For solving the problems of the technologies described above, the inventive method adopts following technical proposals:
A. seed selection meets the single fungi strain or the bacterial classification combination of following condition simultaneously: strain growth speed is fast and can produce a large amount of asexual spores or sexual spore; The active height of the outer pectin degrading enzyme (comprising pectin hydrolase, pectin lyase, pectinesterase and protopectinase) of secreted born of the same parents, cellulase (comprising endoglucanase EC3.2.1.4, glucan excision enzyme EC3.2.1.91 and beta-glucosidase EC3.2.1.21) and hemicellulase; The fungi strain that the oranges and tangerines can is not had quality safety harm;
B. the bacterial classification that makes step a be picked out by fermentation mode produces spore or mycelium;
C. spore or the mycelium that is obtained among the step b is prepared into the microbial degradation agent;
D. with the oranges and tangerines peeling, the distinguish that clean up and remove train of thought on the tangerine lobe, under suitable condition, join in the microbial degradation agent solution, stir and remove the capsule clothing, excystation clothing tangerine lobe.
The bacterial classification of institute's seed selection is a kind of in aspergillus niger, aspergillus flavus and the Jin Mao shell or their composition among the described step a; The microbial degradation agent for preparing among the described step c is: behind the spore on the solid fermentation culture of collecting with the spore gatherer behind the solid fermentation, the solid fermentation solid fermentation culture low temperature drying pulverized the pulvis that is rich in spore that directly obtains or/and directly obtain be rich in mycelial liquid fermentation liquid; In the described steps d microbial degradation agent solution for spore or/and be rich in suspension that the pulvis of spore is mixed with or/and be rich in mycelial liquid fermentation liquid; Can in the microbial degradation agent solution, add the nutriment that can promote spore or mycelial growth in the described steps d; Described nutriment is sucrose, NaNO
3, K
2HPO
4, MgSO
4.7H
2O and (NH
4)
2SO
4In a kind of or their composition; Described suitable condition is 25～60 ℃ of pH value 3～10 and temperature.
Advantage of the present invention is: but the 1. microorganism formulation suitability for industrialized production of degraded orange skin is easy to use, does not need the refining step through enzyme preparation, and the product quality of oranges and tangerines can is not produced safety hazard.2. the green barrier that quality safety technology barriers that the harmful substances such as heavy metal of having avoided soda acid excystation clothing to cause are residual and problem of environmental pollution cause, water-saving consumption-reduction has reduced production cost, has realized economic benefit and and the harmony of social benefit.3. the present invention compares with acid-base method, and the nutritive value behind the microbial method excystation clothing is better than traditional acid-base method, and it has kept original local flavor of citrus fruit and color and luster.
The specific embodiment
Example 1: aspergillus niger (Aspergillus niger 3.316 is available from Institute of Microorganism, Academia Sinica), on Czapek ' s agar, carry out the inclined-plane and cultivate, 26 ℃, 72h; First order seed is cultivated: the 250mL triangular flask seed culture medium 50g that packs into, each constituent mass of described seed culture medium consists of: wheat bran 2g, glucose 3g, (NH
4)
2SO
40.15g water 44.85g is 1cm by inoculum concentration
2Czapek ' s agar medium bacterial classification inoculation after the inoculation, at rotating speed 120r/min, is cultivated 96h down for 30 ℃; Secondary seed is cultivated: add the 5Kg seed culture fluid in the 10L seed fermentation jar, each constituent mass of described seed culture fluid consists of: wheat bran 200g, glucose 300g, (NH
4)
2SO
415g and water 4485g add one-level then and cultivate seed 50g at rotating speed 120r/min, cultivate 96h down for 30 ℃; Solid fermentation is cultivated: the 5Kg second class inoculum inserts in the 50Kg solid fermentation culture medium, and described each constituent mass of solid fermentation culture medium consists of: straw powder 12Kg, orange peel powder 2Kg, wheat bran 10Kg, (NH
4)
2SO
40.25Kg with water 25.75Kg, mix, 30 ℃ of constant temperature culture 108h obtain a large amount of spore or mycelium; Further, standby with the low temperature drying of solid fermentation culture medium, pulverizing.
Citrus reticulata 100kg, after fully cleaning, the steam heat blanch, distinguish is also removed train of thought on the tangerine lobe, tangerine lobe 60Kg is standby; Solid fermentation culture medium 6Kg after pulverizing is joined in the physiological saline of 60Kg, the rear suspension liquid warming-in-water to 35 that stirs ℃, add the 60Kg peeling then and remove train of thought tangerine lobe, hydrochloric acid solution adjust pH to 4.5 with 1moL/L, under 35 ℃ of conditions of temperature, constantly stir constant temperature and kept 2 hours, get full excystation clothing tangerine lobe.
Example 2: aspergillus niger (Aspergillus niger 3.316 is available from Institute of Microorganism, Academia Sinica), on Czapek ' s agar, carry out the inclined-plane and cultivate, 26 ℃, 72h; First order seed is cultivated: the 250mL triangular flask seed culture medium 50g that packs into, each constituent mass of described seed culture medium consists of: wheat bran 2g, glucose 3g, (NH
4)
2SO
40.15g, be 1cm by inoculum concentration with water 44.85g
2Czapek ' s agar medium bacterial classification is inoculated, and after the inoculation, is 120r/min at rotating speed, cultivates 96h down for 30 ℃; Secondary seed is cultivated: add the 5Kg seed culture fluid in the 10L seed fermentation jar, each constituent mass of described seed culture fluid consists of: wheat bran 200g, glucose 300g, (NH
4)
2SO
415g and water 4485g add one-level then and cultivate seed 50g at rotating speed 120r/min, cultivate 96h down for 30 ℃; Fermented and cultured: the 5Kg second class inoculum inserts the 50Kg fermentation culture by inoculum concentration 10% (mass/mass), described fermentation culture pH be 5.0 and each constituent mass consist of: straw powder 2Kg, orange peel powder 0.5Kg, wheat bran 1.5Kg, (NH
4)
2SO
40.05Kg, K
2HPO
40.05Kg and water 45.9Kg, rotating speed 120r/min cultivates 108h for 30 ℃, low temperature is preserved, and is stand-by.
Citrus reticulata 100 Kg, after fully cleaning, the steam heat blanch, distinguish is also removed train of thought on the tangerine lobe, tangerine lobe 60Kg is standby; Warming-in-water to 35 ℃ behind 5 times of the zymotic fluid 12Kg thin ups that above-mentioned low temperature is preserved is used the hydrochloric acid solution adjust pH to 4.5 of 1moL/L then, keeps temperature; Peeling is removed train of thought tangerine lobe join in the zymotic fluid of described insulation, under pH value 4.5,35 ℃ of conditions of temperature, constantly stir constant temperature and kept 2 hours, get full excystation clothing tangerine lobe.
Example 3: aspergillus flavus (Aspergillus flavus 3.3554 is available from Institute of Microorganism, Academia Sinica), on Czapek ' s agar, carry out the inclined-plane and cultivate, 26 ℃, 72h; First order seed is cultivated: the 250mL triangular flask seed culture medium 50g that packs into, each constituent mass of described seed culture medium consists of: wheat bran 1g, orange peel powder 0.5g, glucose 1.5g, (NH
4)
2SO
40.15g, be 1cm by inoculum concentration with water 46.85g
2Czapek ' s agar medium bacterial classification inoculation, the inoculation back is 90r/min at rotating speed, cultivates 96h down for 28 ℃; Secondary seed is cultivated: add the 5Kg seed culture fluid in the 10L seed fermentation jar, the quality group of described each component of seed culture fluid becomes: wheat bran 100g, orange peel powder 50g, glucose 150g, (NH
4)
2SO
415g and water 4685g; Add one-level then and cultivate seed 50g, cultivate 96h down for 28 ℃ at rotating speed 90r/min; Fermented and cultured: the 5Kg second class inoculum inserts the 50Kg fermentation culture by inoculum concentration 10% (mass/mass), and described fermentation culture pH is 5.0, and each constituent mass consists of: orange peel powder 0.5Kg, wheat bran 2.5Kg, (NH
4)
2SO
40.05Kg, K
2HPO
40.05Kg and water 46.9Kg, rotating speed 100r/min cultivates 108h for 28 ℃, low temperature is preserved, and is the A zymotic fluid.Gold hair shell (Chaetomiumaureum 3.3783, available from Institute of Microorganism, Academia Sinica) carries out the inclined-plane on the PDA culture medium cultivates, and 30 ℃, 96h; First order seed is cultivated: the 250mL triangular flask seed culture medium 50g that packs into, each constituent mass of described seed culture medium consists of: potato extract 49g, glucose 1g is 1cm by inoculum concentration
2PDA culture medium bacterial classification is inoculated, and the inoculation back is cultivated 108h down for 30 ℃ at rotating speed 100r/min; Secondary seed is cultivated: add the 5Kg seed culture fluid in the 10L seed fermentation jar, each constituent mass of described seed culture fluid consists of: straw powder 300g, soya-bean cake powder 50g, (NH
4)
2SO
425g, CaCl 15g, water 4610g adds one-level then and cultivates seed 50g at rotating speed 100r/min, cultivates 108h down for 30 ℃; Fermented and cultured: the 5Kg second class inoculum inserts the 50Kg fermentation culture by inoculum concentration 10% (mass/mass), and described fermentation culture pH is 5.0, and each constituent mass consists of: straw powder 3Kg, soya-bean cake powder 0.5Kg, (NH
4)
2SO
40.25Kg, CaCl 0.15Kg and water 46.1Kg, rotating speed 120r/min cultivates 108h for 30 ℃, and low temperature is preserved, and is the B zymotic fluid.
Citrus reticulata 100Kg, after fully cleaning, peeling distinguish Ex-all train of thought, it is standby to get tangerine lobe 60Kg; Warming-in-water to 40 ℃ after A zymotic fluid 3Kg and B zymotic fluid 9Kg be mixed into mixed culture fermentation liquid and dilute 5 times is used the hydrochloric acid solution adjust pH to 5.0 of 1moL/L then, keeps temperature; The tangerine lobe of Ex-all train of thought is joined in the above-mentioned mixed culture fermentation liquid of insulation, under pH5.0,40 ℃, constantly stir, constant temperature kept 3 hours, full excystation clothing tangerine lobe.
Claims (7)
1, a kind of method of utilizing bacteria degradation of orange capsule clothes is characterized in that may further comprise the steps:
A. seed selection meets the single fungi strain or the bacterial classification combination of following condition simultaneously: strain growth speed is fast and can produce a large amount of asexual spores or sexual spore; The active height of the outer pectin degrading enzyme of secreted born of the same parents, cellulase and hemicellulase; The fungi strain that the oranges and tangerines can is not had quality safety harm;
B. the bacterial classification that makes step a be picked out by fermentation mode produces spore or mycelium;
C. spore or the mycelium that is obtained among the step b is prepared into the microbial degradation agent;
D. with the oranges and tangerines peeling, the distinguish that clean up and remove train of thought on the tangerine lobe, join in the microbial degradation agent solution under suitable condition, suitable stirring removes the capsule clothing, excystation clothing tangerine lobe.
2, the method for utilizing bacteria degradation of orange capsule clothes according to claim 1 is characterized in that the microbial degradation agent for preparing among the described step c is: behind the spore on the solid fermentation culture of collecting with the spore gatherer behind the solid fermentation, the solid fermentation solid fermentation culture low temperature drying pulverized the pulvis that is rich in spore that directly obtains or/and directly obtain be rich in mycelial liquid fermentation liquid.
3, the method for utilizing bacteria degradation of orange capsule clothes according to claim 2, it is characterized in that microbial degradation agent solution in the described steps d for spore or/and be rich in suspension that the pulvis of spore is mixed with or/and be rich in mycelial liquid fermentation liquid.
4,, it is characterized in that in the microbial degradation agent solution, to add the nutriment that can promote spore or mycelial growth in the described steps d according to claim 1 or the 2 or 3 described methods of utilizing bacteria degradation of orange capsule clothes.
5, the method for utilizing bacteria degradation of orange capsule clothes according to claim 4 is characterized in that described nutriment is sucrose, NaNO
3, K
2HPO
4, MgSO
4.7H
2O and (NH
4)
2SO
4In a kind of or their composition.
6,, it is characterized in that in the described steps d that suitable condition is 25～60 ℃ of pH value 3～10 and temperature according to claim 1 or the 2 or 3 described methods of utilizing bacteria degradation of orange capsule clothes.
7, according to claim 1 or the 2 or 3 described methods of utilizing bacteria degradation of orange capsule clothes, the bacterial classification that it is characterized in that institute's seed selection among the described step a is a kind of in aspergillus niger, aspergillus flavus and the Jin Mao shell or their composition.
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|---|---|---|---|
| CNB2007100344292A CN100546504C (en) | 2007-02-13 | 2007-02-13 | Utilize the method for bacteria degradation of orange capsule clothes |
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|---|---|---|---|
| CNB2007100344292A CN100546504C (en) | 2007-02-13 | 2007-02-13 | Utilize the method for bacteria degradation of orange capsule clothes |
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| CN100546504C CN100546504C (en) | 2009-10-07 |
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Cited By (7)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101803791A (en) * | 2010-03-30 | 2010-08-18 | 浙江省柑桔研究所 | Method for removing segment membranes from citrus |
| CN102078024A (en) * | 2010-11-25 | 2011-06-01 | 青岛康地恩生物科技有限公司 | Liquid complex enzyme for peeling membranes of orange segments, and compounding process and application thereof |
| CN102994400A (en) * | 2012-12-20 | 2013-03-27 | 湖南李文食品有限公司 | Microorganism capable of degrading navel orange segment membrane and enzymic preparation containing microorganism as well as application |
| CN104054907A (en) * | 2014-06-10 | 2014-09-24 | 湖南省农产品加工研究所 | Preparation method of protein feed |
| CN105462866A (en) * | 2015-12-24 | 2016-04-06 | 邵阳学院 | Activation culture medium powder and activation of yeast strain, segment membrance peeling culture medium powder and application |
| CN106262964A (en) * | 2016-08-05 | 2017-01-04 | 安陆市元畈辣业有限公司 | A kind of method removing ginkgo nut episperm |
| CN111500371A (en) * | 2020-05-12 | 2020-08-07 | 浙江工业大学 | Method for extracting orange peel essential oil by using microbial fermentation method |
-
2007
- 2007-02-13 CN CNB2007100344292A patent/CN100546504C/en active Active
Cited By (11)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101803791A (en) * | 2010-03-30 | 2010-08-18 | 浙江省柑桔研究所 | Method for removing segment membranes from citrus |
| CN102078024A (en) * | 2010-11-25 | 2011-06-01 | 青岛康地恩生物科技有限公司 | Liquid complex enzyme for peeling membranes of orange segments, and compounding process and application thereof |
| CN102078024B (en) * | 2010-11-25 | 2013-04-17 | 青岛蔚蓝生物集团有限公司 | Liquid complex enzyme for peeling membranes of orange segments, and compounding process and application thereof |
| CN102994400A (en) * | 2012-12-20 | 2013-03-27 | 湖南李文食品有限公司 | Microorganism capable of degrading navel orange segment membrane and enzymic preparation containing microorganism as well as application |
| CN102994400B (en) * | 2012-12-20 | 2014-06-18 | 湖南李文食品有限公司 | Microorganism capable of degrading navel orange segment membrane and enzymic preparation containing microorganism as well as application |
| CN104054907A (en) * | 2014-06-10 | 2014-09-24 | 湖南省农产品加工研究所 | Preparation method of protein feed |
| CN105462866A (en) * | 2015-12-24 | 2016-04-06 | 邵阳学院 | Activation culture medium powder and activation of yeast strain, segment membrance peeling culture medium powder and application |
| CN105462866B (en) * | 2015-12-24 | 2018-11-20 | 邵阳学院 | The activation culture original washing powder of yeast strain and activation, excystation clothing medium powder and application |
| CN106262964A (en) * | 2016-08-05 | 2017-01-04 | 安陆市元畈辣业有限公司 | A kind of method removing ginkgo nut episperm |
| CN111500371A (en) * | 2020-05-12 | 2020-08-07 | 浙江工业大学 | Method for extracting orange peel essential oil by using microbial fermentation method |
| CN111500371B (en) * | 2020-05-12 | 2022-05-24 | 浙江工业大学 | Method for extracting orange peel essential oil by using microbial fermentation method |
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|---|---|
| CN100546504C (en) | 2009-10-07 |
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