CN100998618A - Method for continuously extracting sea-buckthorn oil and sea buckthorn flavonoid by using supercritical CO2 fluid - Google Patents
Method for continuously extracting sea-buckthorn oil and sea buckthorn flavonoid by using supercritical CO2 fluid Download PDFInfo
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- CN100998618A CN100998618A CN 200610124371 CN200610124371A CN100998618A CN 100998618 A CN100998618 A CN 100998618A CN 200610124371 CN200610124371 CN 200610124371 CN 200610124371 A CN200610124371 A CN 200610124371A CN 100998618 A CN100998618 A CN 100998618A
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- 238000000034 method Methods 0.000 title claims abstract description 16
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- 239000012530 fluid Substances 0.000 title claims description 9
- 229930003935 flavonoid Natural products 0.000 title 1
- 150000002215 flavonoids Chemical class 0.000 title 1
- 235000017173 flavonoids Nutrition 0.000 title 1
- 235000003935 Hippophae Nutrition 0.000 claims abstract description 71
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- 238000000605 extraction Methods 0.000 claims description 43
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- ADRVNXBAWSRFAJ-UHFFFAOYSA-N catechin Natural products OC1Cc2cc(O)cc(O)c2OC1c3ccc(O)c(O)c3 ADRVNXBAWSRFAJ-UHFFFAOYSA-N 0.000 description 1
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- Extraction Or Liquid Replacement (AREA)
Abstract
A process for continuously extracting hippophae oil and hippophae flavone includes such steps as supercritical CO2 extracting from the dried hippohpae fruit or its dregs at 30-50 deg.C under 20-40 MPa for 0.5-1 hr to obtain hippophae oil, filling entraining agent, and continuous extracting for 2-3 hr to obtain hippophae flavone.
Description
Technical field
The invention belongs to deep processing and the comprehensive utilization technique field of secondary product of forestry deep processing and Fructus Hippophae, particularly a kind of supercritical CO of using
2The method of fluid continuous extraction Oleum Hippophae and Fructus Hippophae flavone.
Background technology
Oleum Hippophae is the material general name that extracts from sea buckthorn fruit or seed by the certain physical means, and the Oleum Hippophae that obtains from sea buckthorn fruit is called Oleum Hippophae, and the Oleum Hippophae that obtains from seed of Fructus Hippophae is called Fructrs Hippophae seed oil; It is the natural materials of a kind of high nutrition, high economic worth.According to research report, contain a large amount of nutrient substances in the Oleum Hippophae, the main component that goes out of isolation identification is broadly divided into vitamin, triterpene, phytosterin compound, lipid, phenols and organic acid etc.Oleum Hippophae has the effect of anti-myocardial ischemia, can prolong the mice time-to-live, and myocardial ischemia is also had the certain protection effect; The function that remarkable enhancing nonspecific immunity, humoral immunization and cellular immunization are arranged; Have certain antitumaous effect, can be used as anticancer adjuvant therapy medicaments, the Synergistic anti-cancer medicine, the immunocompetence of raising tumor patient alleviates the detrimental effect of chemotherapy, radiotherapy; Contain several physiological active substances in the Oleum Hippophae, have tangible radiation resistance, can be used as the medicine that prevention and treatment skin and mucosa are subjected to radiation injury, be used for radiation therapy process, to reduce esophagus and the suffered damage of mucosa; The function that remarkable enhancing nonspecific immunity, humoral immunization and cellular immunization are arranged; Useful already Fructus Hippophae among the people is treated digestive system disease, comprises stomach, duodenal ulcer, inflammation, dyspepsia etc.Studies confirm that Oleum Hippophae has tangible hepatoprotective effect, can resist by CCl
4Obstacles such as the hepatocyte metabolism that causes, sugar is synthetic, solidify in sugared source, protein synthesis.
Fructus Hippophae flavone is the unique activity material that extracts from sea buckthorn fruit or exsiccant seabuckthorn fruit peel slag, dry fruit, Ye Zhongzhong, contains Quercetin, isorhamnetin, kaempferol, chlorogenic acid, pigweed glycosides, catechin, astragaloside, white anthocyanidin, guassin, Flos Camelliae Japonicae element, flavonol and a spot of flavanone isoreactivity composition.Fructus Hippophae flavone has blood fat reducing, and vessel softening reduces myocardial oxygen consumption, strengthens cardiac function, and arrhythmia is anti-early rich, dwindles myocardial infarction area, allevating angina pectoris, and enhance immunity is improved liver function, anti-treating respiratory system disease, functions such as radioprotective.
At present Oleum Hippophae and Fructus Hippophae flavone generally will can obtain respectively in two kinds of different technologies, different equipment; Reported and used supercritical CO
2The research of fluid extraction Oleum Hippophae (Liaoning chemical industry, 2006, the 35th the 2nd phase of volume, p77), method was with after the drying, moisture places supercritical CO less than 10% sea-buckthorn pomace
2Extract in the equipment, obtain Oleum Hippophae.Reported also that in addition with ethanol be solvent extraction Fructus Hippophae flavone (Food Science,, the 26th the 6th phase of volume in 2004, p138), method is exsiccant sea-buckthorn pomace is sloughed lipid material in petroleum ether after, soaks in alcohol solvent and extracts, reclaim ethanol then, obtain Fructus Hippophae flavone.
Because molecular structure and the polarity of Oleum Hippophae and Fructus Hippophae flavone differ greatly, generally must adopt diverse ways, in distinct device, finish, long flow path not only, investment greatly, efficient is not high yet.
Summary of the invention
In order to solve above-mentioned the deficiencies in the prior art part, the object of the present invention is to provide a kind of supercritical CO of using
2The method of fluid continuous extraction Oleum Hippophae and Fructus Hippophae flavone.This method can be in same flow process, continuous extraction goes out Oleum Hippophae and Fructus Hippophae flavone.
The objective of the invention is to realize: a kind of supercritical CO of using by following technical proposals
2The method of fluid continuous extraction Oleum Hippophae and Fructus Hippophae flavone comprises the steps: to place supercritical CO with the Fructus Hippophae dry fruit or through exsiccant sea-buckthorn pomace
2In the extraction equipment, extracted 0.5～1 hour, extraction obtains Oleum Hippophae; The extract yield of Oleum Hippophae is 0.2～0.5%.Then to supercritical CO
2Inject entrainer in the extraction equipment, continue extraction 2～3 hours, extraction obtains Fructus Hippophae flavone; The extract yield of Fructus Hippophae flavone is 0.02～0.5%.Described extraction conditions is: 30～50 ℃ of temperature, pressure 20～40MPa, CO
2Flow 15～25kg/h.
In order to realize the present invention better, described extraction conditions is preferably: 30 ℃ of temperature, pressure 20～30MPa, CO
2Flow 15～20kg/h.
Described entrainer comprises methanol, ethanol or water etc.
The present invention compared with prior art has following advantage and beneficial effect: technology of the present invention is simple, can be in same technological process, and disposable acquisition Oleum Hippophae and Fructus Hippophae flavone; The invention solves the problem that Oleum Hippophae and Fructus Hippophae flavone must extract respectively with distinct device, improved production efficiency, reduced investment, have good application prospects.
The specific embodiment
The present invention is described in further detail below in conjunction with embodiment, but embodiments of the present invention are not limited thereto.
Embodiment 1
The sea-buckthorn pomace of 2000 gram moisture content 9% is placed 5 liters of supercritical COs
2In the extraction equipment, at 30 ℃ of temperature, pressure 20MPa, CO
2Flow 15kg/h extracted 0.5 hour, and extraction obtains Oleum Hippophae, and the Oleum Hippophae extract yield is 0.23%.Then, to supercritical CO
2Inject ethanol in the extraction equipment as entrainer, at 30 ℃ of temperature, pressure 20MPa, CO
2Flow 15kg/h continues extraction 2 hours, and extraction obtains Fructus Hippophae flavone, and the Fructus Hippophae flavone extract yield is 0.2%.
Embodiment 2
The Fructus Hippophae dry fruit of 2000 gram moisture content 9% is placed 5 liters of supercritical COs
2In the extraction equipment, at 30 ℃ of temperature, pressure 30MPa, CO
2Flow 20kg/h extracted 0.5 hour, and extraction obtains Oleum Hippophae, and the Oleum Hippophae extract yield is 0.20%.Then, to supercritical CO
2Inject ethanol in the extraction equipment as entrainer, 30 ℃ of temperature, pressure 30MPa, CO
2Flow 20kg/h continues extraction 3 hours, and extraction obtains Fructus Hippophae flavone, and the Fructus Hippophae flavone extract yield is 0.12%.
Embodiment 3
The sea-buckthorn pomace (through super-dry) of 2000 gram moisture content 9% is placed 5 liters of supercritical COs
2In the extraction equipment, at 50 ℃ of temperature, pressure 40MPa, CO
2Flow 25kg/h extracted 1 hour, and extraction obtains Oleum Hippophae, and the Oleum Hippophae extract yield is 0.48%.Then, to supercritical CO
2Inject methanol in the extraction equipment as entrainer, 50 ℃ of temperature, pressure 40MPa, CO
2Flow 25kg/h continues extraction 2.5 hours, and extraction obtains Fructus Hippophae flavone, and the Fructus Hippophae flavone extract yield is 0.12%.
Embodiment 4
The sea-buckthorn pomace (through super-dry) of 2000 gram moisture content 9% is placed 5 liters of supercritical COs
2In the extraction equipment, at 45 ℃ of temperature, pressure 30MPa, CO
2Flow 20kg/h extracted 1 hour, and extraction obtains Oleum Hippophae, and the Oleum Hippophae extract yield is 0.20%.Then, to supercritical CO
2Inject water in the extraction equipment as entrainer, 45 ℃ of temperature, pressure 30MPa, CO
2Flow 20kg/h continues extraction 2 hours, and extraction obtains Fructus Hippophae flavone, and the Fructus Hippophae flavone extract yield is 0.5%.
The foregoing description is a preferred implementation of the present invention; but embodiments of the present invention are not restricted to the described embodiments; other any do not deviate from change, the modification done under spirit of the present invention and the principle, substitutes, combination, simplify; all should be the substitute mode of equivalence, be included within protection scope of the present invention.
Claims (3)
1, a kind of supercritical CO of using
2The method of fluid continuous extraction Oleum Hippophae and Fructus Hippophae flavone is characterized in that comprising the steps: placing supercritical CO with the Fructus Hippophae dry fruit or through exsiccant sea-buckthorn pomace
2In the extraction equipment, extracted 0.5～1 hour, extraction obtains Oleum Hippophae; Then to supercritical CO
2Inject entrainer in the extraction equipment, continue extraction 2～3 hours, extraction obtains Fructus Hippophae flavone; Described extraction conditions is: 30～50 ℃ of temperature, pressure 20～40MPa, CO
2Flow 15～25kg/h.
2, a kind of supercritical CO of using according to claim 1
2The method of fluid continuous extraction Oleum Hippophae and Fructus Hippophae flavone is characterized in that: described extraction conditions is 30 ℃ of temperature, pressure 20～30MPa, CO
2Flow 15～20kg/h.
3, a kind of supercritical CO of using according to claim 1
2The method of fluid continuous extraction Oleum Hippophae and Fructus Hippophae flavone is characterized in that: described entrainer comprises methanol, ethanol or water.
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| CN 200610124371 CN100998618A (en) | 2006-12-22 | 2006-12-22 | Method for continuously extracting sea-buckthorn oil and sea buckthorn flavonoid by using supercritical CO2 fluid |
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| CN 200610124371 CN100998618A (en) | 2006-12-22 | 2006-12-22 | Method for continuously extracting sea-buckthorn oil and sea buckthorn flavonoid by using supercritical CO2 fluid |
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Cited By (17)
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| CN101905090A (en) * | 2010-05-31 | 2010-12-08 | 中国农业大学 | Method for extracting polar or medium polar plant source natural product |
| CN102070591A (en) * | 2011-01-25 | 2011-05-25 | 向华 | Process method for efficiently extracting apigenin from Chinese violet |
| CN102533433A (en) * | 2012-01-30 | 2012-07-04 | 刘正芳 | Method for supercritical extraction of sea buckthorn oil |
| CN102586006A (en) * | 2012-02-29 | 2012-07-18 | 范兆科 | Method for deeply developing and comprehensively utilizing medicinal and edible plant sea buckthorn |
| CN101921693B (en) * | 2009-08-31 | 2012-10-03 | 内蒙古水域山饮品有限责任公司 | Seabuckthorn white wine and production method thereof |
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