CN100569091C - A kind of preparation method of collagen of red fish skin in deep sea - Google Patents
A kind of preparation method of collagen of red fish skin in deep sea Download PDFInfo
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- CN100569091C CN100569091C CNB2007100229129A CN200710022912A CN100569091C CN 100569091 C CN100569091 C CN 100569091C CN B2007100229129 A CNB2007100229129 A CN B2007100229129A CN 200710022912 A CN200710022912 A CN 200710022912A CN 100569091 C CN100569091 C CN 100569091C
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Abstract
The preparation method of a kind of collagen of red fish skin in deep sea of the present invention belongs to marine biotechnology.Comprise: select for use deep-sea snapper skin to clean and shred, utilize NaCl solution to carry out preliminary treatment, the 0.5M acetic acid solution that adds precooling again, after magnetic stirrer, ice bath, ultrasonic wave 200W handles, add NaCl behind the refrigerated centrifuge in the supernatant and saltout, centrifugal again, precipitation is dissolved with acetic acid solution again, the dialysis freeze drying can obtain white collagen of red fish skin in deep sea solid.The present invention has made full use of aquatic resources, and the processing of aquatic products discarded object is turned waste into wealth, and has both reduced the pollution to environment, has extended industrial chain again, has improved the aquatic products added value; Production technology is simple, strong operability, and cost is low; Collagen productive rate and purity are higher, can be widely used in fields such as functional food, medical material, biological medicine, cosmetics, leather.
Description
One, technical field
The preparation method of a kind of collagen of red fish skin in deep sea of the present invention belongs to marine biotechnology, and product can be widely used in fields such as functional food, medical material, biological medicine, cosmetics, leather.
Two, technical background
China is a fishery big country, and the aquatic products total output had reached 5,102 ten thousand tons in 2005, and the processing of aquatic products amount has also reached 1,195 ten thousand tons.In the process of processing of aquatic products, produced a large amount of discarded objects, as skin, squama, bone, head, internal organ etc., these discarded objects have accounted for the 50%-70% of processing of aquatic products total amount.Though these discarded objects have higher nutritive value, these discarded objects are mainly as low value-added feed, fertilizer etc. at present.In addition, the improper processing of these discarded objects also can cause serious environmental to pollute.Therefore, develop the processing waste of these aquatic products, improve its added value, extend the aquatic products processing industrial chain, huge economic benefit and social benefit are arranged.
Collagen is a kind of structural proteins, be the holder and the filler of tissue, participate in migration, differentiation and propagation, the reparation of wound and embryo's the growth etc. of cell, be used widely in fields such as functional food, medical material, biological medicine, cosmetics, leathers.But the collagen main source is a mammal at present, as skin of pig, ox etc., the pre-treatment that this class collagen extracts is cumbersome, cost is also higher relatively, the particularly outburst of diseases such as aftosa, rabid ox disease, Streptococcus suis in recent years, allow people that the product of pig Niu Laiyuan has been produced very big worry, come the application of product-derived also to be restricted owing to some religion reasons cause pig in addition.Therefore, people are badly in need of seeking the new alternative source of collagen.
The processing of aquatic products discarded object, as bone, skin, scale and fin etc., contain abundant collagen, all can be used as the good substitute of the collagen in livestock products source, still present domestic people mainly concentrate on the livestock products to the research of collagen, research to the processing of aquatic products discarded object is just at the early-stage, and it utilizes more almost is blank.Because the physics of the collagen in livestock products sources and chemical property exist significant difference with the collagen that aquatic products are originated, be widely used in the pig of curriery, the preparation method of cow leather collagen obviously is not suitable for the aquatic products collagen.Therefore, develop a kind of aquatic products collagen efficient production technology, from the processing of aquatic products discarded object, extract collagen and be used, significant.
Three, summary of the invention
Technical problem
The purpose of this invention is to provide a kind of high efficiency method that from the red water fish processing waste of deep-sea, prepares collagen.This method is introduced the extraction process of collagen with ultrasonic technology, adopts the response surface method to optimize the fish-skin preliminary treatment and collagen prepares key process parameter, obtained a kind of from seawater fish processing waste the processing technology of efficient production collagen.Technology is simple, strong operability, and cost is low, the efficient height.
Technical scheme
A kind of preparation method of collagen of red fish skin in deep sea is characterized in that:
1) raw material: remove fish scale and hypodermis on deep-sea snapper (Sebastes mentella) skin, clean up, drain, shred 2 * 2-2 * 5mm with scissors with running water;
2) preliminary treatment: get the 10g fish-skin, add 200-300mL 1.0M NaCl solution, behind the homogeneous 2-5min, use magnetic stirrer 12-24h, use 3 μ m strainer filterings then, and wash 3 times with running water;
3) preparation of collagen: the 0.5M acetic acid solution that in pretreated fish-skin, adds the 350-400mL precooling, after in the refrigeration refrigerator-freezer, using magnetic stirrer 2-6h, ice bath, ultrasonic wave 200W handles 40-60min, use magnetic stirrer 17-21h again, 15000-20000g refrigerated centrifuge 40-60min then, slowly adding NaCl to 0.9M in supernatant saltouts, spend the night, the centrifugal 15-30min of 2500-5000g precipitates the 0.1-0.5M acetic acid solution dissolving with the 30-50mL precooling again, dialyses 2-3 days, freeze drying can obtain pure white collagen of red fish skin in deep sea solid 4.5-5.0g.All operations all carries out in 4 ℃ of refrigerating chambers, and preparation technology can amplify by same ratio.。
Beneficial effect
The present invention relates to a kind of preparation method of collagen of red fish skin in deep sea, from collagen raw material screening, collagen preparation method relatively screen, the development of the aspects such as optimization of collagen preparation technology key parameter, the characteristic of the collagen in deep-sea snapper different tissues (skin, squama, bone) source has been compared in research, the characteristic of the collagen that different preparation methods such as acid system, alkaline process, enzyme process are prepared comprises productive rate, purity, heat endurance, structure etc.The result shows that the productive rate of collagen (47.5%) is significantly higher than fish scale and fish-bone collagen (2.6%, 3.9%), and fish-skin is a preparation collagen optimal material.The quality of solubility in acid collagen is higher, by improving and optimizing the collagen extraction process, improves the solubility in acid collagen production, is the optimal selection of preparation collagen.Ultrasonic technology is introduced the collagen extraction process, and optimization has obtained the best preparation technology of ultrasonic wave assisted extraction collagen.
The present invention has made full use of aquatic resources, and the processing of aquatic products discarded object is turned waste into wealth, and has both reduced the pollution to environment, has extended industrial chain again, has improved the aquatic products added value; Production technology is simple, strong operability, and cost is low, the efficient height; Collagen productive rate and purity higher (with the hydroxyproline content evaluation) can be widely used in fields such as functional food, medical material, biological medicine, cosmetics, leather.
Four, description of drawings
Fig. 1 different N aCl concentration is to the influence of fish-skin pretreating effect
Five, Fa Ming the specific embodiment
Remove fish scale and hypodermis on deep-sea snapper (Sebastes mentella) skin, clean up, drain, shred (2 * 5mm) with scissors with running water.Get the 10g fish-skin, add 300mL 1.0M NaCl solution, behind the homogeneous 5min, use magnetic stirrer 24h, use 3 μ m strainer filterings then, and wash 3 times with running water.
The 0.5M acetic acid solution that in pretreated fish-skin, adds the 400mL precooling, behind magnetic stirrer 6h, ice bath, ultrasonic wave (200W) is handled 60min, use magnetic stirrer 17h again, 15000g refrigerated centrifuge 60min then, slowly adding NaCl to 2.0M in supernatant saltouts, spend the night, the centrifugal 15min of 5000g precipitates the 0.5M acetic acid solution dissolving with the 30-50mL precooling again, dialysis 72h (12h * 6), freeze drying can obtain pure white deep-sea snapper collagen solid 4.5-5.0g, and hydroxyproline (Hpro) content is 76.3mg/gdry wt..All operations all carries out in 4 ℃ of refrigerating chambers.
This technology is fit to produce in batches, only needs to amplify in proportion to get final product.
Claims (2)
1, a kind of preparation method of collagen of red fish skin in deep sea is characterized in that:
1) raw material: remove fish scale and hypodermis on deep-sea snapper (Sebastes mentella) skin, clean up, drain, shred 2 * 5mm with scissors with running water;
2) preliminary treatment: get the 10g fish-skin, add 300mL 1.0M NaCl solution, behind the homogeneous 5min, use magnetic stirrer 24h, use 3 μ m strainer filterings then, and wash 3 times with running water;
3) preparation of collagen: the 0.5M acetic acid solution that in pretreated fish-skin, adds the 400mL precooling, behind magnetic stirrer 6h, ice bath, ultrasonic wave 200W handles 60min, use magnetic stirrer 17h again, 15000g refrigerated centrifuge 60min then, slowly adding NaCl to 2.0M in supernatant saltouts, spend the night, the centrifugal 15min of 5000g precipitates the 0.5M acetic acid solution dissolving with the 30-50mL precooling, dialysis 72h again, freeze drying can obtain pure white deep-sea snapper collagen solid.
2, according to the preparation method of the described a kind of collagen of red fish skin in deep sea of claim 1, it is characterized in that: all operations all carries out in 4 ℃ of refrigerating chambers.
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| CNB2007100229129A CN100569091C (en) | 2007-05-25 | 2007-05-25 | A kind of preparation method of collagen of red fish skin in deep sea |
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| CN100569091C true CN100569091C (en) | 2009-12-16 |
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Families Citing this family (12)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101230088B (en) * | 2008-02-22 | 2011-08-03 | 四川大学 | Method for extracting native natural collagen from animal skin or/and tendon |
| CN101735304B (en) * | 2008-11-26 | 2012-06-06 | 大连隆合海洋生物工程技术有限公司 | Method for preparing water-based collagen of codfish skin by ultrasound wave extraction |
| JP5277480B2 (en) * | 2009-07-27 | 2013-08-28 | 國立成功大學 | Preparation of high purity collagen |
| CN101628938B (en) * | 2009-08-12 | 2012-08-08 | 江苏省海洋资源开发研究院 | Extraction technology of macromolecular collagen protein |
| CN101999992A (en) * | 2010-10-26 | 2011-04-06 | 威海市宇王集团有限公司 | Fish scale collagen cosmetic |
| CN104220586B (en) * | 2010-12-30 | 2017-05-17 | 韩国食品研究院 | Method for extracting collagen using ultrasonic waves, and apparatus therefor |
| CN102863064B (en) * | 2012-09-28 | 2017-05-10 | 江苏艾特克环境工程有限公司 | Method for preparing coagulant for sewage treatment |
| CN103172729A (en) * | 2013-03-11 | 2013-06-26 | 北京航空航天大学 | Method for rapidly preparing fish collagen |
| CN104225689A (en) * | 2013-06-08 | 2014-12-24 | 北京航空航天大学 | Preparation method of fish collagen bone-induced regeneration membrane |
| WO2015012682A2 (en) * | 2013-07-22 | 2015-01-29 | Universiti Putra Malaysia | A method for extracting collagen from aquatic animals, collagen and products containing it |
| CN103980360A (en) * | 2014-05-29 | 2014-08-13 | 杭州市农业科学研究院 | Rapid preparation method of fish scale collagen protein |
| CN108186613A (en) * | 2018-02-12 | 2018-06-22 | 重庆医科大学附属永川医院 | It is a kind of to be used to repair implantation film of bone injury and preparation method thereof |
-
2007
- 2007-05-25 CN CNB2007100229129A patent/CN100569091C/en not_active Expired - Fee Related
Non-Patent Citations (7)
| Title |
|---|
| Biochemical properties of bone and scale collagensisolated from the subtropical fish black drum (Pogonia cromis)andsheepshead seabream (Archosagus probatocephalus). Masahiro Ogawa etc.Food Chemistry,Vol.88 . 2004 |
| Biochemical properties of bone and scale collagensisolated from the subtropical fish black drum (Pogonia cromis)andsheepshead seabream (Archosagus probatocephalus). Masahiro Ogawa etc.Food Chemistry,Vol.88. 2004 * |
| Characterisation of acid-soluble collagenfrom skin and bone of bigeye snapper(Priacanthus tayenus). Phanat Kittiphattanabawon etc.Food Chemistry,Vol.89 . 2005 |
| Isolation and characterisation ofacid and pepsin-solubilised collagens from the skinof Brownstripe red snapper (Lutjanus vitta). Akkasit Jongjareonrak etc.Food Chemistry,Vol.93 . 2005 |
| 淡水鱼鱼皮胶原蛋白的提取. 傅燕凤等.上海水产大学学报，第13卷第2期. 2004 |
| 草鱼皮酸溶性和酶溶性胶原蛋白的提取及性质. 张建忠等.食品科学，第27卷第12期. 2006 |
| 草鱼皮酸溶性和酶溶性胶原蛋白的提取及性质. 张建忠等.食品科学，第27卷第12期. 2006 * |
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