CN100561190C - Dark current detection device and absorbency detection method - Google Patents
Dark current detection device and absorbency detection method Download PDFInfo
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- CN100561190C CN100561190C CNB2006100626523A CN200610062652A CN100561190C CN 100561190 C CN100561190 C CN 100561190C CN B2006100626523 A CNB2006100626523 A CN B2006100626523A CN 200610062652 A CN200610062652 A CN 200610062652A CN 100561190 C CN100561190 C CN 100561190C
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Abstract
The invention discloses a kind of dark current detection device and absorbency detection method, comprise light source, optical filter wheel and pick-up unit, described pick-up unit is used for receiving optical signals, and export corresponding electric signal, described optical filter wheel is provided with the light through hole, on the described smooth through hole optical filter is housed, described optical filter wheel is rotatable so that light leads to the light through hole on the light path of light from the light source to the pick-up unit; Also be provided with lighttight dark current on the described optical filter wheel and measure the position, described dark current is measured the position and can be rotated on the light path of light from the light source to the pick-up unit with optical filter wheel.The present invention comes shading light by the dark current measurement position that is positioned on the optical filter wheel, need not turn off the light and to measure dark current, and can carry out the test of dark current in real time, stabilization time need not be waited for, when test data is handled, what deduct is real-time dark current value, has improved the accuracy of absorbance.
Description
[technical field]
The present invention relates to a kind of dark current detection device and absorbency detection method, can be applicable to microplate reader, biochemical instruments and spectrophotometer etc. needs in the system of deduction dark current influence.
[background technology]
In medical field or optical research, need to measure some physical quantitys of different wavelengths of light usually, measuring method normally is converted to light signal the electric signal input, utilizes the curtage value to come the calculating optical physical quantity.But when measuring, pass through even without light, also can detect electric current, this electric current is called dark current.Be that example describes below with the microplate reader.
Microplate reader is under specific wavelength of light, detect the light absorption value of measured object, its optical system generally includes light source, optical filter wheel (built-in optical filter), optical fiber, composition such as ELISA Plate and pick-up unit, its principle is: during detection, earlier take turns the optical filter of selecting different wave length by the rotating filtering sheet, light source light obtains the light of specific wavelength behind optical filter, light is divided into eight light beams by optical fiber then, the light direct beam of each bundle light is to enzyme mark hole, the light that penetrates enzyme mark hole installs absorption after testing, and pick-up unit is converted to the computational analysis that the electric signal input processing device carries out absorbance with light signal.The intrinsic output of system when dark current enters for no light signal, in the system testing, absorbance is calculated the measured value that needs the deduction dark current, so the accuracy that dark current is measured directly has influence on the result of calculation of absorbance and the performance index of system.The method that the measurement of existing dark current is mainly taked to turn off the light and measured.During start, before not opening illuminator, carry out the test of dark current, and outcome record is got off.After each test was finished, the dark current value that measures when using start was carried out the calculating of absorbance.Yet dark current has drift unavoidably in test process, and prior art is used is the dark current value that once test obtains of starting shooting, if the basis that the dark current value of measuring when using start is calculated as absorbance, can bring the influence of the drift of dark current into, cause that the system testing result's is not accurate enough the absorbance accuracy.Therefore, be necessary to carry out in real time the test of dark current, eliminate the harmful effect of dark current drift absorbance result of calculation.And prior art is because what adopt is the way of turning off the light and measuring, and illuminator is opened again closing after and needed the regular hour to make illuminator stable, if frequent turning off the light, turn on light, this is unacceptable in reality is tested.Therefore this dark current is measured and can only be carried out when start, and measurement that can't be real-time.
[summary of the invention]
Fundamental purpose of the present invention is exactly in order to solve prior art problems, a kind of dark current detection device is provided, before each the detection, all detect dark current, realize the real-time detection of dark current, when calculating absorbance, to remove the influence of dark current drift the absorbance measuring accuracy.
Another object of the present invention just provides a kind of method of utilizing above-mentioned dark current detection device to carry out absorbance detection, before each the detection, all detect dark current, realize the real-time detection of dark current, when calculating absorbance, to remove the influence of dark current drift to the absorbance measuring accuracy.
For achieving the above object, the present invention proposes a kind of dark current detection device, comprise light source, optical filter wheel and pick-up unit, described pick-up unit is used for receiving optical signals, and export corresponding electric signal, described optical filter wheel is provided with the light through hole, on the described smooth through hole optical filter is housed, and the rotatable so that light through hole of described optical filter wheel is positioned on the light path of light from the light source to the pick-up unit; Also be provided with lighttight dark current on the described optical filter wheel and measure the position, described dark current is measured the position and can be rotated on the light path of light from the light source to the pick-up unit with optical filter wheel.
Described smooth through hole and dark current measure the position be preferably be evenly distributed on the concentric circle of optical filter wheel on.
Also comprise condenser, described condenser is positioned at light from light source to the light through hole or on the light path of dark current measurement position.
For achieving the above object, the present invention also proposes a kind of method of utilizing above-mentioned dark current detection device to carry out absorbance detection, may further comprise the steps for each measurement:
A1, rotating filtering sheet wheel make dark current measure the position and are positioned on the light path of light from the light source to the pick-up unit, carry out the measurement of dark current;
B1, rotating filtering sheet wheel make the light through hole that the specified wavelength optical filter is installed be positioned on the light path of light from the light source to the pick-up unit measurement light source background values;
C1, the light through hole that the specified wavelength optical filter is installed is positioned on the light path of light from the light source to the pick-up unit, and the solution that will measure absorbance places container, be sent to the test position, measure the current value of this solution;
The current value of D1, the dark current value of utilizing this measurement, light source background values and this solution calculates this solution absorbency.
Beneficial effect of the present invention:
1, comes shading light by the dark current measurement position that is positioned on the optical filter wheel, obtain the unglazed purpose of passing through, need not turn off the light and to measure dark current, and can carry out the test of dark current in real time, need not wait for stabilization time.
When 2, test data being handled, deduction be real-time dark current value, promptly each absorbance utilization of calculating be the dark current value of this measurement, improved the accuracy of absorbance.
3, the present invention is simple in structure, and light path design need not be changed, and realizes easily.
Feature of the present invention and advantage will be elaborated in conjunction with the accompanying drawings by embodiment.
[description of drawings]
Fig. 1 is the light path system synoptic diagram of an embodiment of the present invention;
Fig. 2 is the optical filter wheel synoptic diagram of an embodiment of the present invention;
Fig. 3 is the absorbance calculation flow chart of an embodiment of the present invention.
[embodiment]
Be that example specifies embodiments of the present invention below with the microplate reader.
The synoptic diagram of the optical system of microplate reader as shown in Figure 1.The light that illuminator 1 sends enters the light through hole 4 of optical filter wheel 3 after condenser 2 converges, the optical filter of different wave length is installed in different light through holes 4.Optical filter wheel 3 is around turning axle 7 rotations, thereby the different light through hole 4 of selection selects different wavelength to test.The light signal of mating plate selection enters the big end 5 of optical fiber after filtration, goes out to inject follow-up light path from optical fiber small end 6.Pick-up unit is used for receiving optical signals, and exports corresponding electric signal, is used for the calculating of follow-up absorbance.
The synoptic diagram of optical filter wheel 3 as shown in Figure 2.On optical filter wheel, a dark current is set measures position 4a.The layout that dark current is measured position 4a and some smooth through holes 4 be preferably be evenly distributed on the concentric circle of optical filter wheel on, make things convenient for the drive of motor and optical filter wheel 3 around turning axle 7 rotations.Dark current is measured position 4a, and a dark current measurement 4a does not install optical filter, but lighttight structure.Measure the position by optical filter wheel being rotated to dark current, be positioned on the light path of light from the light source to the pick-up unit even dark current is measured the position, make that light signal is blocked, subsequent optical path is in no light signal input state, situation when also just being equivalent to illuminator and closing, can measure dark current value this moment.Measure the optical filter wheel of position by adding dark current, having reached does not turn off the light can test the purpose of dark current.
Certainly, dark current is measured other positions that position 4a also can be arranged on optical filter wheel 3.
On this basis, for eliminating the influence of dark current drift to absorbance, the process flow diagram that absorbance is calculated may further comprise the steps as shown in Figure 3:
At first, in each test beginning, optical filter wheel is gone to dark current measure the position, promptly rotating filtering sheet wheel makes dark current measure the position and is positioned on the light path of light from the light source to the pick-up unit, carries out the measurement of dark current;
Then, rotating filtering sheet wheel, the light through hole that the specified wavelength optical filter is installed is positioned on the light path of light from the light source to the pick-up unit, make the light of specified wavelength pass through optical filter wheel, the measurement light source background values, the light source background values is to carry out the benchmark fiducial value that absorbance is calculated, and promptly the light of specified wavelength does not promptly arrive the measured value of pick-up unit through detected solution;
Next step light that still makes specified wavelength is by optical filter wheel, and the solution that will measure absorbance places container, is sent to the test position, measures the current value of this solution correspondence;
The sequencing of above-mentioned steps also can change.
Utilize the current value of dark current value, light source background values and this solution of this measurement to calculate this solution absorbency at last, if dark current value is Y0, the light source background values is Y, and the measured value of solution to be measured is that the computing formula of Yi absorbance I is: I=Log ((Y-Y0)/(Yi-Y0)).
In sum, the present invention can effectively remove the influence of dark current drift to absorbance, and also has same effect for biochemical instruments and spectrophotometer.
Claims (4)
1. dark current detection device, comprise light source, optical filter wheel and pick-up unit, described pick-up unit is used for receiving optical signals, and export corresponding electric signal, described optical filter wheel is provided with the light through hole, on the described smooth through hole optical filter is housed, the rotatable so that light through hole of described optical filter wheel is positioned on the light path of light from the light source to the pick-up unit; It is characterized in that: also be provided with lighttight dark current on the described optical filter wheel and measure the position, described dark current is measured the position and can be rotated on the light path of light from the light source to the pick-up unit with optical filter wheel.
2. dark current detection device as claimed in claim 1 is characterized in that: described smooth through hole and dark current measure the position be evenly distributed on the concentric circle of optical filter wheel on.
3. dark current detection device as claimed in claim 2 is characterized in that: also comprise condenser, described condenser is positioned at light from light source to the light through hole or on the light path of dark current measurement position.
4. utilize the method for carrying out absorbance detection as each described dark current detection device in the claim 1 to 3, it is characterized in that: may further comprise the steps for each measurement:
A1, rotating filtering sheet wheel make dark current measure the position and are positioned on the light path of light from the light source to the pick-up unit, carry out the measurement of dark current;
B1, rotating filtering sheet wheel make the light through hole that the specified wavelength optical filter is installed be positioned on the light path of light from the light source to the pick-up unit measurement light source background values;
C1, the light through hole that the specified wavelength optical filter is installed is positioned on the light path of light from the light source to the pick-up unit, and the solution that will measure absorbance places container, be sent to the test position, measure the current value of this solution;
The current value of D1, the dark current value of utilizing this measurement, light source background values and this solution calculates this solution absorbency.
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| CNB2006100626523A CN100561190C (en) | 2006-09-15 | 2006-09-15 | Dark current detection device and absorbency detection method |
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| CNB2006100626523A CN100561190C (en) | 2006-09-15 | 2006-09-15 | Dark current detection device and absorbency detection method |
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| CN100561190C true CN100561190C (en) | 2009-11-18 |
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| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102221605B (en) * | 2010-04-16 | 2014-12-24 | 深圳迈瑞生物医疗电子股份有限公司 | Sampling system, sampling method and medical treatment analysis meter |
| CN102236039A (en) * | 2010-04-27 | 2011-11-09 | 上海永创医疗器械有限公司 | Dark current detection method |
| CN103048277B (en) * | 2012-12-14 | 2015-08-12 | 北京农业智能装备技术研究中心 | The system and method for near infrared spectrum Non-Destructive Testing fruit internal quality |
| CN103645373B (en) * | 2013-12-27 | 2016-05-11 | 百力达太阳能股份有限公司 | A kind of method of monitoring dark current |
| CN103954819B (en) * | 2014-03-28 | 2016-08-17 | 中国科学院半导体研究所 | Nearly zero partially time semiconductor infrared photodetector surfaces dark current measuring method |
| CN113340856B (en) * | 2021-06-03 | 2022-12-06 | 武汉致腾科技有限公司 | Noise removal algorithm of optical sensor and visibility calculation method thereof |
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Application publication date: 20080319 Assignee: Shenzhen Mindray Animal Medical Technology Co.,Ltd. Assignor: SHENZHEN MINDRAY BIO-MEDICAL ELECTRONICS Co.,Ltd. Contract record no.: X2022440020009 Denomination of invention: Dark current detection device and absorbance detection method Granted publication date: 20091118 License type: Common License Record date: 20220804 |