CN100554927C - A kind of automatic flow-path separation device - Google Patents
A kind of automatic flow-path separation device Download PDFInfo
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- CN100554927C CN100554927C CNB2005101145545A CN200510114554A CN100554927C CN 100554927 C CN100554927 C CN 100554927C CN B2005101145545 A CNB2005101145545 A CN B2005101145545A CN 200510114554 A CN200510114554 A CN 200510114554A CN 100554927 C CN100554927 C CN 100554927C
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- pipeline
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- sample
- resolution element
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Abstract
The present invention relates to a kind of automatic flow-path separation device, it comprises solvent bottle, infusion pump, six-way valve, resolution element, reaches sample bottle; Described solvent bottle connects described infusion pump by pipeline, described six-way valve connects described resolution element by pipeline, it is characterized in that: it also comprises three T-valve that connect described six-way valve respectively by pipeline, and ram pump and has sampling concurrently and is collected in sample collection device all over the body; An outlet of first described T-valve connects a sewer pipe, and another outlet connects described infusion pump by pipeline; Two import and export in addition of second described T-valve connect described ram pump and solvent bottle by pipeline respectively; Two import and export in addition of the 3rd described T-valve connect described resolution element and sample collection device by pipeline respectively.The present invention has not only overcome fussy hand operation in the prior art, and can improve analysis efficiency and analysis result repeatable accuracy, and stream is simple simultaneously.
Description
Technical field
The present invention relates to the separation flow circuit device that uses in the processes such as a kind of biological or chemical sample separation, preparation, particularly about a kind of automatic flow-path separation device.
Background technology
(with the chromatograph is example to existing common separation flow circuit device, as shown in Figure 1) mainly comprise solvent bottle 1, woven hose 2, infusion pump 4, woven hose 5, six-way valve 8, woven hose 13, resolution element 14 (chromatographic column), woven hose 15, detecting device 16, woven hose 19, the gatherer 20 that connects successively, and sample bottle 23 etc.In the analytic process, sample introduction, collection generally are to carry out loaded down with trivial details operation by craft, and analysis efficiency is low, repeatable accuracy is poor.And existing automatic flow-path separation device complex structure, the instrument manufacturing cost is very high and difficult in maintenance.
Summary of the invention
At the problems referred to above, the objective of the invention is the stream of above-mentioned separation flow circuit device is improved, make its switching by various valves, can realize the separation flow circuit device of control automatically.
For achieving the above object, the present invention takes following technical scheme: a kind of automatic flow-path separation device, and it comprises solvent bottle, infusion pump, six-way valve, resolution element, reaches sample bottle; Described solvent bottle connects described infusion pump by pipeline, described six-way valve connects described resolution element by pipeline, it is characterized in that: it also comprises three T-valve that connect described six-way valve respectively by pipeline, and ram pump and has sampling concurrently and is collected in sample collection device all over the body; An outlet of first described T-valve connects a sewer pipe, and another outlet connects described infusion pump by pipeline; Two import and export in addition of second described T-valve connect described ram pump and solvent bottle by pipeline respectively; Two import and export in addition of the 3rd described T-valve connect described resolution element and sample collection device by pipeline respectively.
On the pipeline between described resolution element and the 3rd T-valve, be connected a detecting device.
Described resolution element comprises and can or dissolve in the chromatographic column that the biased sample of solvent separates with the liquid mixing sample, or gel column, ion exchange column, the column jecket that the counter-current separation method is used, the distributing trough that the centrifugal partition chromatograph method is used, the kapillary that the capillary electrophoresis separation sample is used, a kind of in the solvent distillation post.
Therefore the present invention can pass through the switching of control T-valve, six-way valve owing in existing separation flow circuit device, increased by three T-valve, ram pump and sampling/gatherers, and increased electric controller; To the control of ram pump and sampling/gatherer, make the present invention finish automatically sample quantitative sampling, cleaning, sample separation, collect, abandon five processes.So not only overcome fussy hand operation in the prior art, and can improve analysis efficiency and analysis result repeatable accuracy.
Description of drawings
Fig. 1 is that separation flow circuit device of the prior art constitutes synoptic diagram
Fig. 2 is a formation synoptic diagram of the present invention
Fig. 3 is an another embodiment of the present invention
Fig. 4 is a control circuit block diagram of the present invention.
Embodiment
As shown in Figure 1 and Figure 2, flow path system of the present invention removes solvent bottle 1, pipeline 2, infusion pump 4, pipeline 5, six-way valve 8, pipeline 13, resolution element 14, pipeline 15, detecting device 16, pipeline 19, the gatherer 20 that connects successively that comprise same as the prior art, and beyond the sample bottle 23, principal character is to have increased by 6,10,18 and ram pumps 12 of three T-valve, and gatherer 20 is to have sampling concurrently and be collected in sampling/gatherer 24 all over the body simultaneously.Wherein, first T-valve 6 connects infusion pump 4, connects a six-way valve 8 and a sewer pipe 21 by pipeline 7 by pipeline 5 respectively; Second T-valve 10 connects solvent bottle 1, connects six-way valve 8 and be connected ram pump 12 by pipeline 11 by pipeline 9 by pipeline 3 respectively; The 3rd T-valve 18 passes through pipeline 17 connection detectors 16 respectively and is connected sampling/gatherer 24 by pipeline 19.
As shown in Figure 3, in the foregoing description,, can not use detecting device 16, just resolution element 14 directly connects T-valve 18 by pipeline 15 if determined under the condition of disengaging time.
As Fig. 2, shown in Figure 4, in the foregoing description, each T- valve 6,10,18, six-way valve 8, ram pump 12 all can be controlled each motor-driven by driving circuit, and such as being driven T-valve 6 by motor M 1 to connect six-way valve 8, the solvent of control whole instrument is supplied with or discharged; Motor M 2 drives T-valve 10 and links to each other with solvent bottle 1, ram pump 12 and six-way valve 8, finish at sample introduction with when cleaning sample introduction needle to sample quantitatively extraction and solvent streams to control; Solvent when motor M 3 drives T-valve 18 control sample introductions and separated and collected and sample flow to.Motor M 4 drives six-way valve 8 controls and external sample is transferred to the assembly of analyzing stream; Motor M 5 drives the cooperation of ram pump 12 and T-valve 10, finishes the function of quantitative extraction solvent and sample.Solvent bottle 1 is stored and is provided solvent as the system flow phase, and infusion pump 4 inlets are connected with solvent bottle 1 by pipeline 2, and infusion pump 4 can be opened all the time, extracts solvent and supplies with the constant mobile solvent of whole piping flow; Resolution element 14 is assemblies of sample separation; Detecting device 16 is assemblies of test sample.Sampling/gatherer 24 that the present invention adopts is prior art parts, and it both can form sampling components with sample bottle 23, can form collection assembly with the receiving flask (not shown) again, and receiving flask is collected is sample component after separating.
In the foregoing description, sample introduction, elution analysis and the requirement that needs test sample to collect per sample can start different motor realizations by general logic control and driving circuit according to the order of stream operation, is not characteristics of the present invention, do not repeat them here.
Below just the dynamic process that need finish of the present invention describe, the present invention includes: system's cleaning, draw samples, sample separation, collect and abandon five dynamic processes (as shown in Figure 2):
When 1, automatic flow-path separation device is in original state, the solvent path direction is: solvent bottle 1-pipeline 2-infusion pump 4-pipeline 5-T-valve 6-pipeline 7-six-way valve 8-pipeline 13-resolution element 14-pipeline 15-detecting device 16-pipeline 17-T-valve 18-pipeline 19-sampling/gatherer 24, this cleans state for the sample analysis pipeline.
2, when automatic flow-path separation device is in the draw samples state, sampling/gatherer 24 inserts sample bottle 23, motor M 3 drives T-valve 18 and makes pipeline 19,22 are communicated with, the reversing bar connecting pipeline 22 that motor M 4 drives in the six-way valve 8,9, motor M 2 drives T-valve 10 and makes pipeline 9,11 are communicated with, motor M 5 drives ram pump 12 and does the pull action, the solvent path direction is: sample bottle 23-sampling/gatherer 24-pipeline 19-T-valve 18-pipeline 22-six-way valve 8-pipeline 9-T-valve 10-pipeline 11-ram pump 12, through a pull of ram pump 12, just can reach the purpose of automatic ration sampling by the switching of reversing bar in the six-way valve 8.
3, automatic flow-path separation device is in when washing the sample lines state, sampling/gatherer 24 shifts out sample bottle 23, T-valve 18 is motionless, motor M 4 drives six-way valve 8 commutations and makes its interior reversing bar connecting pipeline 7,13, sample separation is carried out in preparation, motor M 2 drives T-valve 10 switchings and makes pipeline 3,9,11 all are communicated with, motor M 5 drives ram pump 12 and does the pull action, the solvent path direction is: solvent bottle 1-pipeline 3-T-valve 10-pipeline 9-six-way valve 8-pipeline 22-T-valve 18-pipeline 19-sampling/gatherer 24, the waste liquid of washing sample lines is entered waste liquid pool, ram pump 12 repeatedly pull several times so that sample lines cleans up.
When carrying out sample drawing and washing sample lines, the state of T-valve 6 is in pipeline 7 closing state all the time, and the solution path direction is: solution bottle 1-pipeline 2-infusion pump 4-pipeline 5-T-valve 6-sewer pipe 21, waste liquid is introduced waste liquid pool.
When 4, automatic flow-path separation device is in the sample separation state, the pipeline 3,9 of T-valve 10 is closed, the pipeline 21 that motor M 1 drives T-valve 6 is closed, pipeline 7 is opened, and the solvent path direction is: solution bottle 1-pipeline 2-infusion pump 4-pipeline 5-T-valve 6-pipeline 7-six-way valve 8-pipeline 13-resolution element 14-pipeline 15-detecting device 16-pipeline 17-T-valve 18-pipeline 19-sampling/gatherer 24.Observe detecting device 16 in elution process, unwanted component is introduced waste liquid pool, this is for abandoning; Peak value occurs in case find the component that needs, just begin to collect the sample component that is detected with receiving flask.
5, after first sample separation is collected and finished, get back to original state again, carry out the sample analysis pipeline and clean.Sample to remainder repeats aforementioned detachment process one by one then, till whole sample separation finish.
Under situation, can remove detecting device in above-mentioned each stream without detecting device.
Claims (6)
1, a kind of automatic flow-path separation device, it comprises solvent bottle, infusion pump, six-way valve, resolution element, reaches sample bottle; Described solvent bottle connects described infusion pump by pipeline, described six-way valve connects described resolution element by pipeline, it is characterized in that: it also comprises three T-valve that connect described six-way valve respectively by pipeline, and ram pump and has sampling concurrently and is collected in sample collection device all over the body; An outlet of first described T-valve connects a sewer pipe, and another outlet connects described infusion pump by pipeline; Two import and export in addition of second described T-valve connect described ram pump and solvent bottle by pipeline respectively; Two import and export in addition of the 3rd described T-valve connect described resolution element and sample collection device by pipeline respectively.
2, a kind of automatic flow-path separation device as claimed in claim 1 is characterized in that: be connected a detecting device on the pipeline between described resolution element and the 3rd T-valve.
3, a kind of automatic flow-path separation device as claimed in claim 1 or 2 is characterized in that: described resolution element comprises can be with the chromatographic column of liquid mixing sample separation.
4, a kind of automatic flow-path separation device as claimed in claim 1 or 2, it is characterized in that: described resolution element comprises can be with the gel column of liquid mixing sample separation, ion exchange column, the column jecket that the counter-current separation method is used, the distributing trough that the centrifugal partition chromatograph method is used, the kapillary that the capillary electrophoresis separation sample is used, a kind of in the solvent distillation post.
5, a kind of automatic flow-path separation device as claimed in claim 1 or 2 is characterized in that: described resolution element comprises the chromatographic column that the biased sample that is dissolved in solvent can be separated.
6, a kind of automatic flow-path separation device as claimed in claim 1 or 2, it is characterized in that: described resolution element comprises the gel column that the biased sample that is dissolved in solvent can be separated, ion exchange column, the column jecket that the counter-current separation method is used, the distributing trough that the centrifugal partition chromatograph method is used, the kapillary that the capillary electrophoresis separation sample is used, a kind of in the solvent distillation post.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CNB2005101145545A CN100554927C (en) | 2005-10-25 | 2005-10-25 | A kind of automatic flow-path separation device |
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| Application Number | Priority Date | Filing Date | Title |
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| CNB2005101145545A CN100554927C (en) | 2005-10-25 | 2005-10-25 | A kind of automatic flow-path separation device |
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| CN1955712A CN1955712A (en) | 2007-05-02 |
| CN100554927C true CN100554927C (en) | 2009-10-28 |
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Families Citing this family (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101571460B (en) * | 2009-06-18 | 2011-08-24 | 中国原子能科学研究院 | Sampling device for small-volume samples |
| CN101598739B (en) * | 2009-07-20 | 2012-05-16 | 中国检验检疫科学研究院 | Full-automatic modular multi-sample intelligent processing system |
| CN101601967B (en) * | 2009-07-20 | 2011-06-22 | 中国检验检疫科学研究院 | Four-channel full automatic gel permeation purification system for macromolecule |
| CN104707359A (en) * | 2013-12-11 | 2015-06-17 | 北京思践通科技发展有限公司 | Automatic solid phase extraction apparatus |
| CN103868970B (en) * | 2014-03-21 | 2016-01-06 | 上海通微分析技术有限公司 | Full-automatic high precision capillary electrophoresis apparatus |
Citations (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US4724081A (en) * | 1986-04-28 | 1988-02-09 | Soken Kagaku Kabushiki Kaisha | Process and apparatus for separation by liquid chromatography |
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Patent Citations (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US4724081A (en) * | 1986-04-28 | 1988-02-09 | Soken Kagaku Kabushiki Kaisha | Process and apparatus for separation by liquid chromatography |
Non-Patent Citations (2)
| Title |
|---|
| 微型电动色谱***的设计. 姚珈等.分析仪器，第2001年第4期. 2001 |
| 微型电动色谱***的设计. 姚珈等.分析仪器，第2001年第4期. 2001 * |
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| CN1955712A (en) | 2007-05-02 |
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