CN100553459C - A kind of pokeberry extract and new purposes thereof - Google Patents
A kind of pokeberry extract and new purposes thereof Download PDFInfo
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- CN100553459C CN100553459C CNB2008101016783A CN200810101678A CN100553459C CN 100553459 C CN100553459 C CN 100553459C CN B2008101016783 A CNB2008101016783 A CN B2008101016783A CN 200810101678 A CN200810101678 A CN 200810101678A CN 100553459 C CN100553459 C CN 100553459C
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Abstract
The present invention discloses a kind of pokeberry extract and preparation method thereof; The present invention also discloses the new purposes of mite extremely of pokeberry extract.
Description
Technical field
The present invention relates to a kind of plant extracts and preparation method thereof, particularly a kind of pokeberry extract and preparation method thereof and new purposes.
Background technology
Human in long agricultural history process, striving to find always various ways with anti-ly effectively cure the disease, pests such as worm, grass, mouse.But the most successfully controlling pest is after the development of 20 middle of century chemical pesticides.As the product of science, agricultural chemicals has brought very big interests to people in control pest, minimizing input, additional income, promotion agricultural development, but also exists corresponding ecological risk simultaneously.Certainly, owing to there are problems such as operation technique, people only pay attention to its favourable one side in the process of using agricultural chemicals in addition, do not pay attention to " 3E " (promptly rendeing a service Effectiveness efficient Efficency and environment Environment) problem of chemical control in activity in production
[2], abuse organic insecticide in a large number, caused the appearance of " 3R " (being residual Residue, rampant again Resurgence and pesticide resistance Resistance) problem again inevitably, therefore, ecological problem has obtained people and has paid close attention to widely.People recognize the novel pesticide of present exploitation must have safe, residual low, nuisanceless, biologically active is high, use the characteristic that rate is low, selectivity is high, has also recognized that agricultural chemicals should be by weak point, flat, fast single effect to multi-faceted development quick-acting, that hold effect, aftereffect simultaneously.Along with the development of science and society, people no longer pay attention to " killing " (killing), but pay attention to " adjusting " (regulation).Wish that promptly the agricultural chemicals of being developed has the selectivity of height, can effectively control the target pest population, safer to non-target organism, easy to use, safety relatively in production, processing, transporting procedures, degraded and can obviously not influence the biorational agricultural chemicals (Biorational insecticides) of environmental quality easily under field conditions (factors).Confirm to show through the scientists years of researches, most plant corpuss contain the insect poisoning, inhibiting secondary substance grows, this be plant in evolution of long period of time, obtain avoid a kind of defence mechanism that various insects are caused harm, these secondary metabolites often have necessarily professional, determined it low, had safety preferably environment and person poultry toxicity.Its another characteristics are exactly that form with multiple composition exists and concurs, and therefore, insect is difficult for developing immunity to drugs to it.In addition, as pesticide plant, take from nature and be applied to nature, its toxic component can be degraded in nature quickly, does not have residue problem.Therefore, in today of paying attention to environmental protection and quality of life day by day, the exploitation vegetable insecticide just has even more important meaning.
After World War II, invention along with DDT and hexachlorcyclohexane, the kind of agricultural chemicals (comprise desinsection, sterilization, kill mite, nematicide, kill mouse, weeding and growth regulator etc.) increases to 900 kinds from 30 kinds to 1971 in 1936, and its output in the U.S. from 100,000,000 pounds to 1971 1,100,000,000 pounds in 1936. the harm of using agricultural chemicals not eliminate destructive insects so in large quantities, cause the problem of wildness and environmental pollution etc. again of pests such as insect on the contrary, the most outstanding with agricultural evil mite especially.Have advantages such as wide spectrum, low toxicity, safety in view of botanical pesticide, all contain the acaricidal activity material in the many plants of natural world, and the miticide proportion is very little in the middle of the botanical pesticide, compare with the demand in market, the acaricidal research and development of plant resource still are in the junior stage, become the very few especially of product, therefore acaricidal research and development have great potential and urgency to plant resource.Aspect the research of natural acaricidal activity material, many scholars have done a large amount of work both at home and abroad, have found much to have the material of acaricidal activity.At present, the relatively more sufficient acaricidal activity material of research mainly comprises alkaloids, citrin class, flavonoids, plants essential oil etc.
On the crops of phytophagous mites all over the world discovery being arranged all, have characteristics such as volume is little, breeding is fast, adaptability develops immunity to drugs by force and easily, is the pest group of the very difficult control of generally acknowledging.The present used most dependence on import of miticide on the agricultural chemicals market of China, the acaricidal expense of import account for that the general import agricultural chemicals expense uses 88.8%.And the loss that crop is caused of mite class, tangible increase was arranged in the past in 30 years.Main cause is intensive monoculture and uses non-selectivity insecticide.Next is because the growth time of a generation of mite class is shorter, can produce resistance rapidly to original miticide.These insecticides often acaricidal activity are not high, eliminated mite class natural enemy when killing the mite class yet.At present, the 3R problem of chemical pesticide generally is subjected to people's attention, and human more and more deep to the understanding of agricultural chemicals, the use of agricultural chemicals is not paid attention to killing off the insect pests yet, but more pays attention to regulating.Therefore, use the obligate miticide to become the major measure of control phytophagous mites, inevitable biopesticide and the insect hormone type agricultural chemicals direction towards efficient, wide spectrum, low toxicity, safety of acaricidal development develops, and satisfies the demand in market.
Summary of the invention
The object of the invention is to provide pokeberry extract; Another purpose of the present invention is to provide the Phytolacca acinosa preparation method of extract; The object of the invention also is to provide the new purposes of mite extremely of pokeberry extract.
The present invention seeks to be achieved through the following technical solutions:
Extract of the present invention is made by following method:
Take by weighing the Phytolacca acinosa plant drymeal, add benzinum, chloroform or methyl alcohol that dry powder 3-7 doubly measures, the difference lixiviate is 2-4 time under the room temperature, and each 3~5 days, concentrating under reduced pressure merged, and obtains extract.
Method for preparing extractive of the present invention is preferably:
Take by weighing the Phytolacca acinosa plant drymeal, add the benzinum of 5,6 or 4 times of amounts of dry powder, the difference lixiviate is 2,3 or 4 times under the room temperature, and each 3~5 days, conventional concentrating under reduced pressure merged, and obtains ligroin extraction.
Solvent benzinum in the said method can also be replaced with chloroform and methyl alcohol and obtain chloroform extract, methanolic extract respectively.Above-mentioned pokeberry extract can be made miticide with conventional method.
Following experimental example or embodiment are used to further specify but are not limited to the present invention.
1 materials and methods
1.1 material
1.2 extracting method
Take by weighing the Phytolacca acinosa plant drymeal, add benzinum, chloroform or the methyl alcohol of 5 times of amounts of dry powder, the difference lixiviate is 3 times under the room temperature, and each 3~5 days, concentrating under reduced pressure merges 3 times extract, and was standby.
1.3 indoor bioactive mensuration
Slide infusion process a: end that active, the of the same size female one-tenth mite of health back is sticked at slide downwards with two-sided tape paper, and under anatomical lens, reject the killed or wounded individuality, guarantee that every slide tetranychid number is 30, take out after in soup, soaking 5s, sop up floating medicine gently with filter paper, observe its death condition behind 24h, the moving person of contact is for living, motionless person is dead, the calculation correction lethality.
2 results and analysis
The Phytolacca acinosa plant extracts sees Table 2 and table 3 to the biologically active of the female one-tenth mite of tetranychus viennensis, as can be seen from the table:
Three kinds of extracts of Equisetaceae plant pokeweed all have certain biologically active to tetranychus viennensis, and wherein the biologically active of benzinum is higher, and the 24h corrected mortality is 85.72%, LC
50Be 0.52mg/ml; The methanolic extract acaricidal activity is lower, and the 24h corrected mortality is 55.72%, and the acaricidal activity of chloroform extract is between between the two, and the 24h corrected mortality is 71.45%.
Table 2 pokeberry extract is to the biologically active of the female one-tenth mite of tetranychus viennensis
* every kind of reagent test concentration is 2mg/ml, 3 repetitions, and contrast is 1% Tween 80 solution
Table 3 Phytolacca acinosa plant extracts is to the virulence equation of tetranychus viennensis
* every kind of reagent is 5 concentration, 3 repetitions; * LC
50Unit is mg/ml, and each is handled and repeats 3 times, and contrast is 1% Tween 80 solution.
3 conclusions
The biologically active of plant pokeweed ligroin extraction is higher, and the 24h corrected mortality is 85.72%, LC
50Be 0.52mg/ml.
Following embodiment all can realize the effect of above-mentioned experimental example
Embodiment
Embodiment 1: pokeberry extract and preparation method thereof
Take by weighing the Phytolacca acinosa plant drymeal, add the benzinum of 5 times of amounts of dry powder, the difference lixiviate is 2 times under the room temperature, and each 4 days, conventional concentrating under reduced pressure merged extract, gets ligroin extraction, and above-mentioned pokeberry extract can be made miticide with conventional method.
Embodiment 2: pokeberry extract and preparation method thereof
Take by weighing Pokeberry Root, add the chloroform of 4 times of weight of dry powder, the difference lixiviate is 4 times under the room temperature, and each 3 days, concentrating under reduced pressure merged, and gets chloroform extract, and above-mentioned pokeberry extract can be made miticide with conventional method.
Embodiment 3: pokeberry extract and preparation method thereof
Take by weighing Pokeberry Root, add the benzinum of 6 times of weight of dry powder, the difference lixiviate is 3 times under the room temperature, and each 5 days, concentrating under reduced pressure merged, and gets methanolic extract, and above-mentioned pokeberry extract can be made miticide with conventional method.
Claims (4)
1, the application of pokeberry extract in the preparation miticide, pokeberry extract is made by following method:
Take by weighing the Phytolacca acinosa plant drymeal, add benzinum, chloroform or methyl alcohol that dry powder 3-7 doubly measures, the difference lixiviate is 2-4 time under the room temperature, and each 3~5 days, concentrating under reduced pressure merged, and obtains extract.
2, the application of pokeberry extract according to claim 1 in the preparation miticide, pokeberry extract is made by following method:
Take by weighing Pokeberry Root, add the benzinum of 5 times of weight of dry powder, the difference lixiviate is 3 times under the room temperature, and each 4 days, concentrating under reduced pressure merged, and gets ligroin extraction.
3, the application of pokeberry extract as claimed in claim 2, the benzinum that it is characterized in that making in this pokeberry extract method obtains chloroform extract, methanolic extract respectively with chloroform and methyl alcohol replacement.
4,, it is characterized in that described mite extremely is meant tetranychus viennensis according to the application of the arbitrary described pokeberry extract of claim 1-3 in the preparation miticide.
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CNB2008101016783A CN100553459C (en) | 2008-03-11 | 2008-03-11 | A kind of pokeberry extract and new purposes thereof |
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CNB2008101016783A CN100553459C (en) | 2008-03-11 | 2008-03-11 | A kind of pokeberry extract and new purposes thereof |
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CN101411341A CN101411341A (en) | 2009-04-22 |
CN100553459C true CN100553459C (en) | 2009-10-28 |
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Families Citing this family (4)
Publication number | Priority date | Publication date | Assignee | Title |
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CN102002090B (en) * | 2010-11-23 | 2012-10-03 | 苏州派腾生物医药科技有限公司 | Preparation method of Ombuoside H |
CN104522059B (en) * | 2014-12-30 | 2017-02-22 | 中南林业科技大学 | Phytolacca americana extract and application of phytolacca americana extract in preventing and treating dalbergia odorifera anthracnose |
CN106234426A (en) * | 2016-07-15 | 2016-12-21 | 广西顺帆投资有限公司 | One vegetative insecticide and preparation method thereof |
CN113785845A (en) * | 2021-09-18 | 2021-12-14 | 河南省林业科学研究院 | Plant extract with antibacterial effect and application thereof |
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Non-Patent Citations (2)
Title |
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中药化学. 肖崇厚,16-18,上海科学技术出版社. 2003 |
中药化学. 肖崇厚,16-18,上海科学技术出版社. 2003 * |
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