CN100547403C - A kind of micro-fluidic chip analyzer and support chip - Google Patents
A kind of micro-fluidic chip analyzer and support chip Download PDFInfo
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- CN100547403C CN100547403C CNB2005100902603A CN200510090260A CN100547403C CN 100547403 C CN100547403 C CN 100547403C CN B2005100902603 A CNB2005100902603 A CN B2005100902603A CN 200510090260 A CN200510090260 A CN 200510090260A CN 100547403 C CN100547403 C CN 100547403C
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Abstract
The present invention relates to a kind of mobile micro-fluidic chip analyzer of laser radiation driving testing liquid and supporting special chip of adopting, described micro-fluidic chip analyzer accurately shines surface, chip analysis workspace scheduled circuit by controllable light source, make surface, chip analysis workspace possess hydrophilic property, thereby the guiding testing liquid is controlled, reversible mobile at chip surface, and can obtain the testing liquid of determining volume.But this micro-fluidic chip analyzer and chip large-scale application are in required quick, integrated check and analysis in aspect such as clinical, food, medicine, chemical industry, environment, herding, agriculturals, compare with prior art micro-fluidic chip analytical technology, need on chip, not process intensive microchannel, have simple, the advantage of low manufacturing cost of chip structure.
Description
Technical field
The present invention relates to a kind of micro-fluidic chip analyzer, relate in particular to and adopt laser radiation to drive mobile micro-fluidic chip analyzer of testing liquid and supporting special chip.
Background technology
At present, micro-fluidic chip is to be architectural feature with the microchannel network, and its material is selected and channels designs is the key factor of micro-fluidic chip and the technical development of corresponding analysis instrument.Initial micro-fluidic chip is to utilize technology such as photoetching, wet corrosion technology, inductively coupled plasma etching to etch the microchannel on glass, quartz, silicon chip.But, with glass, quartz, silicon chip be matrix make micro-fluidic chip technical matters complexity, tediously long, step is loaded down with trivial details, cost is high.In recent years, micro-fluidic chip adopts macromolecular material more, and for example polymethylmethacrylate of rigidity (PMMA), flexible dimethyl silicone polymer (PDMS) and poly-carbon ester (PC) etc. are to utilize low etc. the advantage of its handling ease, cost.Macromolecular material is that the micro-fluidic chip of matrix adopts pressing mold usually, injection moulding, and methods such as X-ray etching or laser ablation are made.
Therefore the micro-fluidic chip of above-mentioned prior art all need be made a large amount of microchannels in the job analysis district, and 96 microchannels of processing on a sheet base for example exist manufacturing technology complexity, processing step is many, cost of manufacture is high shortcoming.In addition, in the prior art micro-fluidic chip analyzer liquid flow to be measured is adopted high-voltage electric field or pump drive source, increased the complexity of chip structure, also increased difficulty liquid driven control on the chip.
Therefore, need a kind of simple in structure, cost of manufacture is low, the simple accurate micro-fluidic chip of liquid flow control and corresponding analyser.
Summary of the invention
The object of the invention provides a kind of micro-fluidic chip analyzer and supporting micro-fluidic chip special, can overcome structure that the prior art micro-fluidic chip exists, complex manufacturing technology, and the defective of liquid flow drive controlling difficulty.
The basic structure of at first introducing micro-fluidic chip analyzer of the present invention and special chip with and principle of work.Micro-fluidic chip of the present invention utilizes the insulating material surface to be filled with the variation of water logging lubricant nature behind the electric charge, make surface, chip analysis workspace possess hydrophilic property, thereby the guiding testing liquid flows at chip surface.And to be filled with the process of electric charge be to carry out by micro-fluidic chip analyzer of the present invention on this insulating material surface, specifically, controllable light source in the analyser is accurately shone chip surface, realize that water infiltration sexually revises on the chip surface projected route, realize the controllability that testing liquid flows thus; Can adopt simultaneously charged the light-guide material of below, zone of another controllable light source irradiation, change its conductivity, can make this charging zone by its surface hydrophobic of ground connection discharge recovery.Two kinds of method combinations can realize that testing liquid is controlled, reversible mobile, and testing liquid are partitioned into volume required by the mode that adopts the siphon chamber.
First kind of micro-fluidic chip of the present invention, substrate at optical glass or transparent plastic material, for example polycarbonate plastic (PC) or polyester plastics (PET) surface, pass through vacuum magnetic-control sputtering, surface filming technology such as vacuum chemistry deposition, form transparent conductive film, this layer nesa coating adopts for example tin indium oxide material, on this layer nesa coating, deposit the ground floor light-guide material then, this ground floor light-guide material can adopt for example selen-tellurjum alloy material, it is inner along presenting low-resistance physical characteristics with the laminar surface vertical direction that this layer light-guide material has under bluish-green laser irradiation material layer, deposit the second layer photovoltaic material with photovoltaic property on above-mentioned ground floor light-guide material again, this second layer photovoltaic material adopts for example titania (TiO
2) material, this second layer photovoltaic material has illuminated surface charging under the ultraviolet laser irradiation and presents hydrophilic physical characteristics, being provided with plastics on second layer photovoltaic material is the double faced adhesive tape liner plate layer of base material, double faced adhesive tape liner plate layer has the thickness less than 0.2 millimeter, this double faced adhesive tape liner plate layer is the rectangular ring with breach, the second layer photovoltaic material upper surface that is surrounded in the straight-flanked ring is the analytical work district, apply one deck Silan-based Thin Films or tetrafluoroethene film at second layer photovoltaic material upper surface analytical work district inside surface, make the analytical work district on the second layer photovoltaic material have strong-hydrophobicity, on the double faced adhesive tape liner plate cover plate is set, but cover plate adopts the transparent material of optical quartz glass or other transparent, UV light beam, Acrylite for example, in the part of cover plate lower surface correspondence analysis workspace also silane coating film or tetrafluoroethene film, make the part of cover plate lower surface correspondence analysis workspace have strong-hydrophobicity, form in the analytical work district height thus smaller or equal to 0.2 millimeter cavity.The cover plate upper surface adds telltale mark by screen printing mode, is used for chip and positions when analyser uses.The substrate and the cover plate that utilize the double faced adhesive tape liner plate will deposit nesa coating, ground floor light-guide material, second layer photovoltaic material and apply hydrophobic film in the analytical work district are combined closely and are interfixed.Second layer photovoltaic material upper surface outside ring-type double faced adhesive tape breach has kept the liquid storage district, and this liquid storage district is used for testing liquid is directly instiled should the zone at chip, then chip is placed on analyser and carries out test analysis.
In above-mentioned first kind of micro-fluidic chip of the present invention, the rectangular ring parts that surround the analytical work district also can be by photoetching, the 0.2 millimeters thick colloidal materials that processing technologys such as pressing mold will have a certain degree of hardness for example photoresist is molded directly within second layer photovoltaic material upper surface, obtain to bind the rectangular ring parts of the colloidal materials layer formation that is one with second layer photovoltaic material layer, and form height smaller or equal to cavity above 0.2 millimeter the analytical work district, second layer photovoltaic material upper surface applies the hydrophobic material film in the analytical work district then, combines fixing with the colloidal materials layer with cover plate.
During use, chip is placed on the loading worktable of analyser of the present invention, this loading worktable is by two step motor drive, this loading worktable can be placed several chips according to actual needs and test, for example put six chips, by the ground floor transparent conductive film ground connection of analyser with micro-fluidic chip, the loading worktable is respectively arranged with upper table and lower table up and down in the analyser, upper table is by two step motor drive, so that upper table accurately moves in two dimensional surface together with the ultraviolet laser head of being installed, make the ultraviolet laser head of being installed on the upper table accurately aim at required irradiation position in the analytical work district, the analytical work district that is radiated at chip second layer photovoltaic material of the present invention after the transparent cover is crossed in the focusing ultraviolet laser transmission that the ultraviolet laser hair goes out, fixed point forms the surface charging zone on second layer photovoltaic material surface, this charging zone makes relevant position, surface, analytical work district form hydrophilic region, lower table is accurately moved in two dimensional surface by two step motor drive equally, the blue green light laser head of being installed on the control lower table accurately moves so that aim at specified position on the ground floor light-guide material layer, after the focusing blue green light laser-transmitting that the blue green light laser head sends is crossed substrate and nesa coating, be radiated on the ground floor light-guide material, in light-guide material, form along low-resistance fixed point zone on this layer thickness direction, because this low resistance zone makes the charging charge of second layer photovoltaic material on correspondence position on this ground floor light-guide material discharge through ground floor low resistance light-guide material and nesa coating ground connection, thereby make analytical work district upper surface forfeiture water wettability and recover strong-hydrophobicity, because little height cavity of top, analytical work district partly forms the siphon passage based on the capillary principle, forming hydrophilic area by accurately moving of control ultraviolet laser head with accurately moving of blue green light laser head moves continuously, thereby the guiding testing liquid enters the analytical work district from the liquid storage district, and according to the moving line of ultraviolet laser head make testing liquid analytical work district surface controlled flow to assigned address and form controlled distribution.In the use of chip of the present invention, the chemical analysis reagent of predetermined fixed drying regime on the precalculated position in the analytical work district on the chip, the position of analytical reagent corresponds to the telltale mark of cover plate upper surface, specifies the chemical analysis reagent position to carry out biochemical reaction so that analyser can be directed to testing liquid according to telltale mark in analytical test.Behind the reaction beginning certain hour, by laser-induced fluorescence (LIF) or chemiluminescence signal are detected, thereby obtain the test result of test substance.The detection probe of test light signal and ultraviolet laser are first with being installed on the upper table, two step motor drive upper tables move in two dimensional surface, carry out light signal strength and measure so that detection probe is accurately moved to target location in the analytical work district.
By adopting micro-fluidic chip of the present invention and adopting the corresponding analysis instrument to carry out above-mentioned control, not only can guide to the place that needs to liquid conductance to be measured, but also can control the size of its distribution.And the guided procedure of this testing liquid is still not controlled, and is reversible.
Second kind of simplification that micro-fluidic chip is first kind of chip of the present invention, owing to adopt the ultraviolet laser irradiation ground floor light-guide material layer upper surface of more high-power for example 0.1W to 1.0W scope, thereby directly near this ground floor light-guide material layer upper surface, produce gas discharge, make near the gas the ground floor light-guide material layer upper surface form plasma, this plasma makes that the hydrophobic material film surface is charged, can be implemented in analytical work district upper surface equally and form hydrophilic area.This second kind of chip technology scheme can be omitted the second layer photovoltaic material layer in the aforementioned chip technology scheme, the double faced adhesive tape liner plate of rectangular ring directly mutually combines the ground floor light-guide material on the substrate and cover plate fixing, and the ground floor light-guide material upper surface that is surrounded at the double faced adhesive tape liner plate still applies with first kind of hydrophobic film that the chip technology scheme is identical.Second kind of chip technology scheme compared with first kind of chip, further facilitating chip structure and manufacturing process.But, this second kind of chip structure is poor slightly in the siphon channel plane dimensional accuracy that surface, analytical work district forms, and the ultraviolet laser head of analyser needs bigger irradiation power, other structure of chip and analyser is formed constant, when carrying out the not high biochemical test of accuracy requirement, use this second kind of chip technology scheme and analyser can further reduce the technology difficulty of chip preparation, reduce the chip preparation cost simultaneously.
Utilize micro-fluidic chip analyzer of the present invention and chip of the present invention, but large-scale application is in required quick, integrated check and analysis in aspect such as clinical, food, medicine, chemical industry, environment, herding, agriculturals, compare with existing conventional micro-fluidic chip analytical technology, owing to need on chip, not process intensive microchannel, have simple, the low cost of manufacture of chip structure, but the advantage of large-scale practical application.Also can be used for other fields such as integrated microreactor.
Description of drawings
Fig. 1 is the first example structure stereographic map of first kind of micro-fluidic chip of the present invention;
Fig. 2 is the second example structure stereographic map of first kind of micro-fluidic chip of the present invention;
Fig. 3 is the first example structure stereographic map of second kind of micro-fluidic chip of the present invention;
Fig. 4 is the structural representation of analyser middle-ultraviolet lamp laser head of the present invention and blue green light laser head;
Fig. 5 is the structural representation of micro-fluidic chip analyzer of the present invention;
Fig. 6 is a loading Working table structure synoptic diagram.
Embodiment
Explain micro-fluidic chip of the present invention and pairing analyser structure in detail below in conjunction with specific embodiment.
Fig. 5 represents the structure of analyser of the present invention.As shown in Figure 5, micro-fluidic chip 1 of the present invention is positioned on the loading worktable 4 of analyser, the loading Working table structure as shown in Figure 6, loading worktable 4 is the rectangular node framework, the rectangular shaped rim that six hollows are arranged, can in frame, inlay six chips of the present invention simultaneously, loading worktable 4 among Fig. 6 has been inlayed chip piece 1 in a frame, loading worktable 4 is driven by stepper motor 41 and stepper motor 42, and the chip that current needs is carried out analytical test is positioned between upper table 2 and the lower table 3; It on the loading worktable 4 upper table 2, upper table 2 is provided with ultraviolet laser head 23 and detection probe 24, upper table 2 is driven in the two dimensional surface parallel with loading worktable 4 by stepper motor 21 and stepper motor 22 and moves, and the ultraviolet laser head 23 on the upper table 2 or detection probe 24 accurately is positioned at the precalculated position of institute's test chip; It under the loading worktable 4 lower table 3, lower table 3 is provided with blue green light laser head 33, lower table 3 is driven in the two dimensional surface parallel with loading worktable 4 by stepper motor 31 and stepper motor 32 and moves, and the blue green light laser head 33 of lower table 3 accurately is positioned at the precalculated position of institute's test chip; Guidance panel 6 is provided with operation push-button and LCD, can be used for moving of manual operation loading worktable 4, ultraviolet laser head 23, blue green light laser head 33, detection probe 24, and on LCD the display analysis result; The operation signal that button produces on the guidance panel 6 passes to control circuit 5, by control circuit 5 operation signal is converted to upper table stepper motor 21 and stepper motor 22, lower table stepper motor 31 and stepper motor 32, the control signal of loading worktable stepper motor 41 and stepper motor 42, in the control, the action of lower table and loading worktable, control circuit 5 is also controlled on the upper table 2 the laser emission output of the blue green light laser head of installing on the ultraviolet laser head 23 installed and the lower table 4 33, and reception is from the detection signal of detection probe 24, and detection signal is converted to the testing result data sends guidance panel to, by the liquid crystal display displays testing result of guidance panel 6; Control circuit 5 also connects IO interface 7, by IO interface 7 receive from external unit for example personal computer the operation steering order of upper table 2, lower table 3 and loading worktable 4 and is exported to for example personal computer of external unit by IO interface 7 with testing result.The detection probe that micro-fluidic chip analyzer of the present invention adopted is made up of optical lens group and photomultiplier, perhaps is made up of optical lens group and charge-coupled image sensor.Micro-fluidic chip of the present invention is provided with on upper table 2 and induces lasing light emitter, for example near ultraviolet light emitting diode (LED) lasing light emitter when adopting the laser-induced fluorescence (LIF) mode to detect.As shown in Figure 4, ultraviolet laser head 23 and blue green light laser head 33 are formed by laser instrument and lens combination.The bluish-green laser of described blue green light laser head emission wavelength ranges 400 to 550nm.
Fig. 1 represents first kind of microfluidic chip structure instantiation of the present invention.As depicted in figs. 1 and 2, the optical glass of rectangular shape or transparent plastic material substrate 101, it on it indium oxide layer tin transparent conductive film 102, shape, size is identical with substrate 101, transparent conductive film 102 exposes rectangular area 110 and 111 as discharge connector in two angular positions of chip length direction one end, be used for the ground connection discharge, last layer is a ground floor light-guide material selen-tellurjum alloy-layer 103 again, light-guide material layer shape, size is except all identical with substrate 101 discharge connector zone 110 and 111, on be second layer photovoltaic material titanium dioxide layer 104, its shape, size is except all identical with substrate 101 discharge connector zone 110 and 111, last layer is that the double faced adhesive tape liner plate 105 of plastic basis material is a rectangular ring again, rectangular ring liner plate 105 1 ends are provided with breach 109, liner plate 105 outline width are identical with optical glass substrate 101, length is less than optical glass substrate 101, thickness is smaller or equal to 0.2 millimeter, liner plate 105 is adhesively fixed on the second layer photovoltaic material layer 104, breach 109 is aimed at the liquid storage district 108 that second layer photovoltaic material floor is provided with near discharge connector zone 110 and 111 1 end upper surfaces, the liner plate other end aligns with the other end of second layer photovoltaic material layer, second layer photovoltaic material layer surface area silane coating film or tetrafluoroethene film that liner plate 105 straight-flanked ring structures are surrounded, as the analytical work district, cover plate 106 is adhesively fixed on the liner plate 105, cover plate 106 is rectangle and length, width and liner plate 105 are equal to and four limits and liner plate 105 positioned in alignment, with screen printing mode telltale mark 107 is set on the cover plate 106, is used for analytic process laser head and detection probe location.As shown in Figure 2, liquid storage district 108 is exposed to second layer photovoltaic material laminar surface, is used to preserve testing liquid.In facture of microchip of the present invention, need the especially physical dimension of substrate and cover plate of accurate control layers of material, scale error must be less than 10 microns.
With blood sugar and uric acid chemical examination is example, illustrates how to utilize micro-fluidic chip of the present invention and corresponding analysis instrument.When utilizing first kind of facture of microchip blood sugar of the present invention and uric acid analysis chip, at first on optical glass substrate 101, form transparent conductive film layer 102, ground floor light-guide material layer 103, second layer photovoltaic material layer 104, the transparent conductive film floor is provided with the discharge connector zone 110 and 111 of two jiaos of leaving exposed of liquid storage district one end at chip, and, form the chip semi-manufacture at predetermined analytical work district silane coating film or tetrafluoroethene film.Select the half-finished limit of rectangular dies as gulde edge, on micro-separatory machine platform, chip is placed on accurate position.With comprise respectively glucose oxidase, peroxidase for example hydrogen peroxidase, luminol and uricase, peroxidase for example several microlitres of the solution of hydrogen peroxidase, luminol drip in the chip second layer photovoltaic material layer hydrophobicity analysis workspace allocation really, above-mentioned chemical analysis reagent is retained in this with drying mode and determines the position after super-dry, and above-mentioned position is called the enzyme point for short.Because chip size is accurately controlled, enzyme point is determined apart from the distance of chip gulde edge like this, keeps enzyme point location parameter to be used for the location of analyser test process laser head and detection probe.Similarly, determine the liquid storage zone position at second layer photovoltaic material layer one end upper surface, and keep corresponding location parameter.Afterwards, on second layer photovoltaic material layer 104, add liner plate 105 and cover plate 106 tight sealing-ins, and the precalculated position has been provided with telltale mark 107 on cover plate 106, determine the relative position of this telltale mark and gulde edge, when chip is placed on the loading worktable of analyser, gulde edge with these telltale mark 107 identification chips, and determine each enzyme point position in analyser according to the location parameter between each enzyme point and the gulde edge, thereby accurately the laser head of control analysis instrument and detection probe are to the enzyme point location.
During assay, after 108 places, liquid storage district of above-mentioned first kind of chip 1 add an amount of test serum, chip is put into analyser, and chip transparent conductive film floor 102 is in the earth lead terminal ground connection of the discharge connector zone 110 and 111 of exposed state by analyser at the end that chip is provided with liquid storage district 108.Analyser is determined the gulde edge of chip by telltale mark 107, and desmoenzyme point location parameter and liquid storage zone position parameter are determined each enzyme point and liquid storage zone position, ultraviolet laser head 23 by upper table 2 focuses on ad-hoc location surface in the irradiation chip analysis workspace, make this surface charging, form the hydrophilic region microchannel, testing liquid flows along the microchannel that hydrophilic region forms, and enters the little height cavity on the chip analysis workspace.Utilize the ultraviolet laser head 23 of upper table 2 to focus on the place not far in the irradiation chip analysis workspace equally apart from enzyme point, form the circular hydrophilic region of 2 millimeters of diameters by mobile ultraviolet laser head 23, on this border circular areas, utilize the height of little height cavity can obtain to determine the test serum of volume.Utilize the blue green light laser head 33 of lower table 3 to focus on the feed liquor microchannel zone of the corresponding above-mentioned border circular areas of irradiation then along circle tangent line place, make this zone, feed liquor microchannel by transparent conductive film 102 and 101,111 discharges of discharge connector zone, the passage area water wettability is disappeared, then the test serum at border circular areas place and the microchannel between the liquid storage district are got in touch and are cut off in the analytical work district, and border circular areas forms relatively independent test serum drop.Still utilize the ultraviolet laser head 23 of upper table 2 to focus on irradiation formation drainage channel, test serum in the border circular areas is drained into enzyme point region, contact with chemical reagent and react, utilize the detection probe 24 on the upper table 2 to carry out luminous intensity measurement behind the certain hour, the light intensity signal of measuring is transferred to control circuit 5, converse blood sugar and uric acid content result data in the test serum by control circuit 5 according to light intensity signal, result data is transferred to guidance panel 6 or exports to external unit by IO interface 7.
Figure 2 shows that second embodiment of first kind of chip structure of the present invention, as shown in Figure 2, wherein directly on second layer photovoltaic material layer, directly be processed to form the gelatinous layer of 0.2 millimeter thickness and one-tenth rectangular ring by modes such as photoetching, pressing molds, this gelatinous layer makes in the present embodiment with photoresist and forms, because gelatinous layer is formed directly into second layer photovoltaic material layer, make the straight-flanked ring and the second layer photovoltaic material floor in the encirclement analytical work district that gelatinous layer constitutes form integrative-structure, the integrative-structure layer shown in Reference numeral among Fig. 2 104/105.The setting of first kind of chip second embodiment chemical analysis reagent shown in Figure 2, location and testing liquid injection mode are all identical with first embodiment, no longer are repeated in this description at this.The use-pattern of first kind of chip second embodiment shown in Figure 2 especially transparent conductive film layer 102 is also identical with first kind of chip first embodiment shown in Figure 1 by the mode of analyser ground connection.
Fig. 3 represents second kind of microfluidic chip structure specific embodiment of the present invention.The difference of second kind of chip structure and first kind of chip shown in Figure 1 is to omit first kind of second layer photovoltaic material layer 104 in the chip technology scheme, the double faced adhesive tape liner plate 105 of rectangular ring mutually combines ground floor light-guide material layer 103 and cover plate 106 fixing, ground floor light-guide material layer 103 surface area of being surrounded at double faced adhesive tape liner plate layer 105 still apply with first kind of hydrophobic material film that the chip technology scheme is identical, and ground floor light-guide material floor 103 upper surface outside the breach 109 of double faced adhesive tape liner plate floor 105 are provided with liquid storage district 108 equally.Second kind of chip technology scheme compared with first kind of chip, owing to omitted second layer photovoltaic material layer 104, thus further facilitating chip structure and manufacturing process.Among Fig. 3 in second kind of chip structure except omitting second layer photovoltaic material layer 104, all the other each layers are all identical with first kind of chip embodiment shown in Figure 1.Above-mentioned second kind of chip of the present invention is provided with chemical analysis reagent and all identical with first kind of chip first embodiment shown in Figure 1 to the method for reagent location positioning in the analytical work district, be not repeated in this description at this.The use-pattern of this second kind of chip of the present invention and first kind of chip are similar, unique difference is that the ultraviolet laser head 23 of analyser upper table 2 of the present invention is coated with the analytical work district of hydrophobic material film with ground floor light-guide material floor 103 upper surface in second kind of chip of higher-wattage irradiation of 0.1 to 1.0 watt, because the higher-wattage laser radiation makes surface, analytical work district air be ionized, form plasma, thereby this plasma makes the charged irradiated area possess hydrophilic property that makes of analytical work district upper surface, all the other analysis operation processes comprise that the earthing mode of chip transparent conductive film layer 102 in analyser is all identical with first kind of chip, the first embodiment use-pattern.
In each chip technology scheme, the layer that same material forms is represented with identical Reference numeral with identical structure division shown in Fig. 1,2,3.Those skilled in the art should be noted that, only to chip material and the laser wavelength that the uses formula explanation of giving an example, anyly can under laser radiation, produce other material of photoconductive property, photovoltaic property and other wave band of laser light source that any irradiation respective material can produce photoconductive property, photovoltaic property all can be used as substituting chip material and the use of substituting analyser light source among this instructions embodiment.For example, when using arsenic selenide (Se-As) material as the material of ground floor light-guide material layer 103, because this arsenic selenide material is for the infrared laser sensitivity, therefore the blue green light laser head on the analyser lower table 3 that the material of arsenic selenide therewith shown in Figure 5 is used as ground floor light-guide material layer chip should replace with the infrared laser head.Except above-mentioned selen-tellurjum alloy material and arsenic selenide material, other light-guide materials for example material that also can be used as chip ground floor light-guide material layer 103 of the present invention such as cadmium sulfide (CdS) material, unformed silicon (A-Si) material and organic light-guide material (OPC) use, and also the laser head that can launch described material sensitive wave length laser should be installed on the lower table 3 of micro-fluidic chip analyzer of the present invention naturally.Similarly, second layer photovoltaic material layer 104 also can use other photovoltaic material beyond the titania, also the laser head that can launch the photovoltaic material sensitive wave length laser that uses should be installed on the upper table 2 of micro-fluidic chip analyzer of the present invention naturally.Transparent conductive film layer 102 in the chip of the present invention also can use other outer transparent conductive materials of tin indium oxide.Chip form of the present invention also is not limited to the rectangular shape shown in Fig. 1 to 3, and those skilled in the art can adopt other chip form as required flexibly.For example, when needs, liquid storage district 108 and breach 109 can all be set, even can also adopt square chip and liquid storage district 108 and breach 109 all are set on square four limits at chip length direction two ends.
Adopt micro-fluidic chip of the present invention and supporting analyser in actual use, the index of test can add as required, integrated, to realize hyperchannel, big flux test.
Claims (8)
1. micro-fluidic chip analyzer, wherein
Described microfluidic analysis instrument comprises:
Loading worktable (4) is latticed rectangular platform, and six hollow square frames wherein are set, and inlays a micro-fluidic chip in each square frame, and loading worktable (4) is driven by the 5th stepper motor (41) and the 6th stepper motor (42);
It is characterized in that: described analyser further comprises:
Upper table (2) is positioned on the loading worktable (4), and upper table (2) drives ultraviolet laser head of installing on the upper table (23) and detection probe (24) by first stepper motor (21) and second stepper motor (22);
Lower table (3) is positioned under the loading worktable (4), and lower table (3) is driven by the 3rd stepper motor (31) and the 4th stepper motor (32), and blue green light laser head (33) is installed on the lower table;
Guidance panel (6), its with control circuit (5) between be connected signal line, guidance panel (6) is provided with button and LCD, be used for the operation signal of the manual button generation of operating personnel to upper table (2), lower table (3), and operation signal passed to control circuit (5), also be used for liquid crystal display displays chemical analysis testing result by guidance panel (6);
Control circuit (5), be used for operation signal is converted to upper table first stepper motor (21) and second stepper motor (22), lower table the 3rd stepper motor (31) and the 4th stepper motor (32), the control signal of loading worktable the 5th stepper motor (41) and the 6th stepper motor (42), control upper table (2), the action of lower table (3) and loading worktable (4), control circuit (5) also is used to control the output of the blue green light laser head of installing on the ultraviolet laser head (23) installed on the upper table and the lower table (33), and receive detection signal, and detection signal is converted to the testing result data sends guidance panel (6) to from detection probe (24); Control circuit (5) also with between the IO interface (7) is connected signal line;
IO interface (7) receives from the operation steering order of external unit to upper table (2), lower table (3) and loading worktable (4), and being transferred to control circuit (5) by signal line, IO interface (7) also will be exported to external unit from the testing result information of control circuit (5);
Micro-fluidic chip comprises:
The substrate of optical glass or transparent optical plastic material (101),
Be layer of transparent conductive film (102) on the substrate, transparent conductive film (102) exposes rectangular area (110) and (111) as discharge connector two jiaos of chip length directions, be used for by the discharge of analyser ground connection,
On the nesa coating ground floor light-guide material (103),
On the ground floor light-guide material second layer photovoltaic material (104),
Being provided with plastics on the second layer photovoltaic material is the double faced adhesive tape liner plate layer (105) of base material, double faced adhesive tape liner plate layer has the thickness less than 0.2 millimeter, this double faced adhesive tape liner plate layer is for having the rectangular ring of breach (109), vertical width equals the vertical width of substrate to rectangular ring double faced adhesive tape liner plate lateral length less than the substrate lateral length
Second layer photovoltaic material (104) upper surface that is surrounded in the rectangular ring double faced adhesive tape is the analytical work district, applies one deck strong-hydrophobicity film on the surface in second layer photovoltaic material upper surface analytical work district,
Transparent material cover plate (106) is set on the double faced adhesive tape liner plate (105), and the part in cover plate (106) lower surface correspondence analysis workspace also applies the strong-hydrophobicity film,
Form in the analytical work district height smaller or equal to 0.2 millimeter cavity by second layer photovoltaic material (104) upper surface, rectangular ring double faced adhesive tape liner plate (105), cover plate (106),
Cover plate (106) upper surface adds telltale mark (107) with screen printing mode,
The substrate (101) that double faced adhesive tape liner plate (105) will deposit nesa coating (102), ground floor light-guide material (103), second layer photovoltaic material (104) and apply hydrophobic film in the analytical work district is combined closely and is interfixed with cover plate (106),
The outer second layer photovoltaic material upper surface of the breach (109) of rectangular ring double faced adhesive tape liner plate (105) has kept liquid storage district (108).
2. according to the described micro-fluidic chip analyzer of claim 1, it is characterized in that:
The described transparent optical plastic material that constitutes substrate (101) is polycarbonate plastic (PC) or polyester plastics (PET),
Described transparent conductive film (102) material on the substrate (101) is a tin indium oxide,
Described ground floor light-guide material (103) is the selen-tellurjum alloy,
Described second layer photovoltaic material (104) is titania (TiO
2),
Applying one deck strong-hydrophobicity film at second layer photovoltaic material (104) upper surface analytical work district inside surface is Silan-based Thin Films or tetrafluoroethene film,
Described transparent material cover plate (106) adopts optical quartz glass or Acrylite,
The strong-hydrophobicity film that part in cover plate (106) lower surface correspondence analysis workspace applies is Silan-based Thin Films or tetrafluoroethene film.
3. micro-fluidic chip analyzer, wherein
Described microfluidic analysis instrument comprises:
Loading worktable (4) is latticed rectangular platform, and six hollow square frames wherein are set, and inlays a micro-fluidic chip in each square frame, and loading worktable (4) is driven by the 5th stepper motor (41) and the 6th stepper motor (42);
It is characterized in that: described analyser further comprises:
Upper table (2) is positioned on the loading worktable (4), and upper table (2) drives ultraviolet laser head of installing on the upper table (23) and detection probe (24) by first stepper motor (21) and second stepper motor (22);
Lower table (3) is positioned under the loading worktable (4), and lower table (3) is driven by the 3rd stepper motor (31) and the 4th stepper motor (32), and blue green light laser head (33) is installed on the lower table;
Guidance panel (6), its with control circuit (5) between be connected signal line, guidance panel (6) is provided with button and LCD, be used for the operation signal of the manual button generation of operating personnel to upper table (2), lower table (3), and operation signal passed to control circuit (5), also be used for liquid crystal display displays chemical analysis testing result by guidance panel (6);
Control circuit (5), be used for operation signal is converted to upper table first stepper motor (21) and second stepper motor (22), lower table the 3rd stepper motor (31) and the 4th stepper motor (32), the control signal of loading worktable the 5th stepper motor (41) and the 6th stepper motor (42), control upper table (2), the action of lower table (3) and loading worktable (4), control circuit (5) also is used to control the output of the blue green light laser head of installing on the ultraviolet laser head (23) installed on the upper table and the lower table (33), and receive detection signal, and detection signal is converted to the testing result data sends guidance panel (6) to from detection probe (24); Control circuit (5) also with between the IO interface (7) is connected signal line;
IO interface (7) receives from the operation steering order of external unit to upper table (2), lower table (3) and loading worktable (4), and being transferred to control circuit (5) by signal line, IO interface (7) also will be exported to external unit from the testing result information of control circuit (5);
Micro-fluidic chip comprises:
The substrate of optical glass or transparent optical plastic material (101),
Be layer of transparent conductive film (102) on the substrate, transparent conductive film (102) exposes rectangular area (110) and (111) as discharge connector two jiaos of chip length directions, be used for by the discharge of analyser ground connection,
On the nesa coating ground floor light-guide material (103),
On the ground floor light-guide material second layer photovoltaic material (104),
Form colloidal materials layer (105) with photoresist on the second layer photovoltaic material, this colloidal materials layer has the thickness less than 0.2 millimeter, this colloidal materials layer is for having the rectangular ring of breach (109), this colloidal materials layer and the second layer photovoltaic material structure (104/105) that is combined as a whole, vertical width equals the vertical width of substrate to the colloidal materials layer lateral length of rectangular ring less than the substrate lateral length
Second layer photovoltaic material (104) upper surface that the colloidal materials floor of rectangular ring is surrounded is the analytical work district, applies one deck strong-hydrophobicity film on the surface in second layer photovoltaic material (104) upper surface analytical work district,
Transparent material cover plate (106) is set on the colloidal materials layer, and the part in cover plate (106) lower surface correspondence analysis workspace also applies the strong-hydrophobicity film,
Form height in the analytical work district smaller or equal to 0.2 millimeter cavity by colloidal materials floor (105), the cover plate (106) of second layer photovoltaic material (104) upper surface, rectangular ring,
Cover plate (106) upper surface adds telltale mark (107) with screen printing mode,
The substrate (101) that colloidal materials floor (105) will deposit nesa coating (102), ground floor light-guide material (103), second layer photovoltaic material (104) and apply hydrophobic film in the analytical work district is combined closely and is interfixed with cover plate (106),
The outer second layer photovoltaic material upper surface of the colloidal materials break mouth (109) of rectangular ring has kept liquid storage district (108).
4. according to the described micro-fluidic chip analyzer of claim 3, it is characterized in that:
The described transparent optical plastic material that constitutes substrate (101) is polycarbonate plastic (PC) or polyester plastics (PET),
Described transparent conductive film (102) material on the substrate (101) is a tin indium oxide,
Described ground floor light-guide material (103) is the selen-tellurjum alloy,
Described second layer photovoltaic material (104) is titania (TiO
2),
Applying one deck strong-hydrophobicity film at second layer photovoltaic material (104) upper surface analytical work district inside surface is Silan-based Thin Films or tetrafluoroethene film,
The material that described colloidal materials layer (105) adopts is a photoresist,
Described transparent material cover plate (106) adopts optical quartz glass or Acrylite,
The strong-hydrophobicity film that part in cover plate (106) lower surface correspondence analysis workspace applies is Silan-based Thin Films or tetrafluoroethene film.
5. micro-fluidic chip analyzer, wherein
Described microfluidic analysis instrument comprises:
Loading worktable (4) is latticed rectangular platform, and six hollow square frames wherein are set, and inlays a micro-fluidic chip in each square frame, and loading worktable (4) is driven by the 5th stepper motor (41) and the 6th stepper motor (42);
It is characterized in that: described analyser further comprises:
Upper table (2), be positioned on the loading worktable (4), upper table (2) is driven by first stepper motor (21) and second stepper motor (22), ultraviolet laser head of installing on the upper table (23) and detection probe (24), and the power of ultraviolet laser head (23) is 0.1 watt to 1.0 watts;
Lower table (3) is positioned under the loading worktable (4), and lower table (3) is driven by the 3rd stepper motor (31) and the 4th stepper motor (32), and blue green light laser head (33) is installed on the lower table;
Guidance panel (6), its with control circuit (5) between be connected signal line, guidance panel (6) is provided with button and LCD, be used for the operation signal of the manual button generation of operating personnel to upper table (2), lower table (3), and operation signal passed to control circuit (5), also be used for liquid crystal display displays chemical analysis testing result by guidance panel (6);
Control circuit (5), be used for operation signal is converted to control signal to upper table first stepper motor (21) and second stepper motor (22), lower table the 3rd stepper motor (31) and the 4th stepper motor (32), loading worktable the 5th stepper motor (41) and the 6th stepper motor (42), the action of control upper table (2), lower table (3) and loading worktable (4), control circuit (5) also is used for receiving the detection signal from detection probe (24), and detection signal is converted to the testing result data sends guidance panel (6) to; Control circuit (5) also with between the IO interface (7) is connected signal line;
IO interface (7) receives from the operation steering order of external unit to upper table (2), lower table (3) and loading worktable (4), and being transferred to control circuit (5) by signal line, IO interface (7) also will be exported to external unit from the testing result information of control circuit (5);
Micro-fluidic chip comprises:
The substrate of optical glass or transparent optical plastic material (101),
On the substrate layer of transparent conductive film (102),
Be ground floor light-guide material (103) on the nesa coating, transparent conductive film (102) exposes rectangular area (110) and (111) as discharge connector two jiaos of chip length directions, be used for by the discharge of analyser ground connection,
Ground floor light-guide material (103) is gone up and is provided with plastics is the double faced adhesive tape liner plate layer (105) of base material, double faced adhesive tape liner plate layer has the thickness less than 0.2 millimeter, this double faced adhesive tape liner plate layer is for having the rectangular ring of breach (109), vertical width equals the vertical width of substrate to rectangular ring double faced adhesive tape liner plate lateral length less than the substrate lateral length
Ground floor light-guide material (103) upper surface that is surrounded in the rectangular ring double faced adhesive tape is the analytical work district, applies one deck strong-hydrophobicity film on the surface in ground floor light-guide material upper surface analytical work district,
Transparent material cover plate (106) is set on the double faced adhesive tape liner plate (105), and the part in cover plate (106) lower surface correspondence analysis workspace also applies the strong-hydrophobicity film,
Form in the analytical work district height smaller or equal to 0.2 millimeter cavity by ground floor light-guide material (103) upper surface, rectangular ring double faced adhesive tape liner plate (105), cover plate (106),
Cover plate (106) upper surface adds telltale mark (107) with screen printing mode,
The substrate (101) that double faced adhesive tape liner plate (105) will deposit nesa coating (102), ground floor light-guide material (103) and apply hydrophobic film in the analytical work district is combined closely and is interfixed with cover plate (106),
The outer ground floor light-guide material upper surface of the breach (109) of rectangular ring double faced adhesive tape liner plate (105) has kept liquid storage district (108).
6. according to the described micro-fluidic chip analyzer of claim 4, it is characterized in that:
The described transparent optical plastic material that constitutes substrate (101) is polycarbonate plastic (PC) or polyester plastics (PET),
Described transparent conductive film (102) material on the substrate (101) is a tin indium oxide,
Described ground floor light-guide material (103) is the selen-tellurjum alloy,
Applying one deck strong-hydrophobicity film at ground floor light-guide material (103) upper surface analytical work district inside surface is Silan-based Thin Films or tetrafluoroethene film,
Described transparent material cover plate (106) adopts optical quartz glass or Acrylite,
The strong-hydrophobicity film that part in cover plate (106) lower surface correspondence analysis workspace applies is Silan-based Thin Films or tetrafluoroethene film.
7. the testing liquid driving method of a micro-fluidic chip, described method are used for driving as testing liquid on the described chip of claim 1 to 4, and described method comprises step:
The precalculated position is provided with chemical analysis reagent in the analytical work district that the hydrophobic material film of micro-fluidic chip photovoltaic material floor upper surface setting forms, and the relative position between definite reagent and the micro-fluidic chip gulde edge;
In the liquid storage district of micro-fluidic chip photovoltaic material floor upper surface setting, inject testing liquid;
Analyze the photovoltaic material layer of position, appointed area in the workspace with the ultraviolet laser irradiation, the charging of photovoltaic material layer, make this appointed area possess hydrophilic property of analytical work district surface, little height space of this hydrophilic region and surface, analytical work district forms siphonic effect, and testing liquid is attracted to flow into this hydrophilic region;
Light-guide material layer with appointed area lower position in the blue green light laser radiation correspondence analysis workspace, make the interior relevant position of light-guide material layer resistance reduce, thereby by the discharge of the transparent conductive film layer ground connection under light-guide material layer and the light-guide material layer, make the appointed area of correspondence analysis workspace lose water wettability and recover hydrophobicity the photovoltaic material layer lotus of charging in the relevant position;
Along alternately irradiation of scheduled circuit, the guiding testing liquid flows into the ad-hoc location that is provided with chemical analysis reagent from the liquid storage district in the analytical work district for ultraviolet laser and blue green light laser.
8. the testing liquid driving method of a micro-fluidic chip, described method are used for driving as testing liquid on the described chip of claim 5 to 6, and described method comprises step:
The precalculated position is provided with chemical analysis reagent in the analytical work district that the hydrophobic material film of micro-fluidic chip light-guide material floor upper surface setting forms, and the relative position between definite reagent and the micro-fluidic chip gulde edge;
In the liquid storage district of micro-fluidic chip light-guide material floor upper surface setting, inject testing liquid;
Analyze the light-guide material layer of position, appointed area in the workspace with the ultraviolet laser irradiation of higher-wattage, light-guide material laminar surface air is ionized the formation plasma, described plasma makes this appointed area, surface, analytical work district charge and possess hydrophilic property, little height space of this hydrophilic region and surface, analytical work district forms siphonic effect, and testing liquid is attracted to flow into this hydrophilic region;
Light-guide material layer with position, appointed area in the blue green light laser radiation correspondence analysis workspace, make the interior relevant position of light-guide material layer resistance reduce, thereby by the discharge of the transparent conductive film layer ground connection under light-guide material layer and the light-guide material layer, make the appointed area of correspondence analysis workspace lose water wettability and recover hydrophobicity light-guide material laminar surface electric charge;
Along alternately irradiation of scheduled circuit, the guiding testing liquid flows into the ad-hoc location that is provided with chemical analysis reagent from the liquid storage district in the analytical work district for ultraviolet laser and blue green light laser.
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| Application Number | Priority Date | Filing Date | Title |
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| CNB2005100902603A CN100547403C (en) | 2005-08-12 | 2005-08-12 | A kind of micro-fluidic chip analyzer and support chip |
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| CNB2005100902603A CN100547403C (en) | 2005-08-12 | 2005-08-12 | A kind of micro-fluidic chip analyzer and support chip |
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| Publication number | Priority date | Publication date | Assignee | Title |
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| CN101923045B (en) * | 2010-05-07 | 2012-02-08 | 重庆大学 | Micro-fluidic chip observing platform and dual-visual field micro-fluidic chip observing system |
| CN102644049A (en) * | 2012-04-26 | 2012-08-22 | 北京工业大学 | Micro-flow driving method based on TiO2 nano-film wettability |
| GB201217344D0 (en) * | 2012-09-28 | 2012-11-14 | Ibm | Microfluidic surface processing systems with self- regulated distance-to surface control |
| CN102921480B (en) * | 2012-10-26 | 2014-10-01 | 北京理工大学 | Method for manufacturing micro-fluidic chip by ultraviolet cured optical cement |
| CN104762344B (en) * | 2015-04-22 | 2018-04-03 | 南京工业大学 | The method that FOS is continuously prepared using immobilized enzyme micro-reactor |
| CN108414773B (en) * | 2018-07-12 | 2018-11-02 | 上海速创诊断产品有限公司 | A kind of micro-fluidic detection chip and preparation method thereof, fixing device and centrifugal detection device |
| CN109254018B (en) * | 2018-09-11 | 2021-12-07 | 华中科技大学同济医学院附属协和医院 | Pharmacokinetic imaging detection system for radiopharmaceuticals |
| WO2021189290A1 (en) * | 2020-03-25 | 2021-09-30 | 京东方科技集团股份有限公司 | Detection chip, detection device, and methods for manufacturing and operating detection chip |
| CN113567397A (en) * | 2021-08-11 | 2021-10-29 | 苏州索真生物技术有限公司 | Microfluidic chip, microfluidic chip channel positioning structure and positioning method |
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