CN100535634C

CN100535634C - Biochip detection method and system with light intensity real-time detection

Info

Publication number: CN100535634C
Application number: CNB2004100090447A
Authority: CN
Inventors: 邢薇; 唐小萍; 岑辉
Original assignee: Institute of Optics and Electronics of CAS
Current assignee: Institute of Optics and Electronics of CAS
Priority date: 2004-04-26
Filing date: 2004-04-26
Publication date: 2009-09-02
Anticipated expiration: 2024-04-26
Also published as: CN1690690A

Abstract

A testing method and its system of biochip with light intensity checking real-time. Add an optical density pan to laser path to adjust light intensity; set light detection system under the plant to detect the light intensity from xenon lamp, the light intensity value reflects truly the real situation of laser light source and is stored in computer; when the value is changed, computer decides according to signal supplied by detection circuit and takes action, adjust light intensity plate or exposure of sample to ensure the reality and reliability of detection data. The invention can adjust light intensity plate, also exposure of biological sample; avoid checking error from light source intensity difference or even variety, ensure the reality and reliability of detection data of biochip detection system.

Description

Have biochip test method and detection system thereof that light intensity detects in real time

Affiliated technical field

The present invention relates to a kind of biochip test method and detection system thereof that light intensity detects in real time that have.

Technical background

In the biological chips detection system testing process; because the serviceable life of exciting light sources xenon lamp and the factors such as instability of line voltage; usually can cause that the light intensity of xenon source and homogeneity change; so not only can make biological chips detection system in testing process because the variation of light source intensity and detection data that the homogeneity difference causes biological chips detection system produce difference; cause misjudgment; and photobleaching can appear, biochip is caused damage.At present, multiple biochip supplies analysis is arranged both at home and abroad, but in these instruments, all do not adopt real-time detection technique of light intensity and adjustment measure, change the influence that is brought to solve light source intensity.

Summary of the invention

Technology of the present invention is dealt with problems and is: for overcome that biological chips detection system occurs in testing process by the caused detection error problem of light source intensity difference, a kind of biological chips detection system that light intensity detects in real time that has is provided, make and in time to be detected when the light source intensity change conditions appears in biological chips detection system in testing process, and in time take measures by computing machine, adjust the exposure of optical density dish or control print, make that the detection data are true and reliable.

The technical solution adopted for the present invention to solve the technical problems is: have the biochip test method that light intensity detects in real time, it is characterized in that: in the exciting light light path of biological chips detection system, add an optical density dish, to adjust light intensity, an optical detection system is installed below slide holder, its objective is the light intensity of the light on the detected print of arrival that detection sent by xenon source, this light intensity value has reflected the truth of excitation source light intensity really, this light intensity value record to some extent in computing machine, when this light intensity value change, computer system is judged according to the signal that testing circuit provides, in time take measures, adjust the exposure of optical density dish or adjustment print, to guarantee to detect the true and reliable of data.

The biological chips detection system of realizing said method and designing with the real-time detection of light intensity, comprise optical system, the emission interference filter, computing machine, slide holder, xenon source, excite interference filter, it is characterized in that: after xenon source, add the optical density dish that is useful on the adjusting light intensity, photo-detector is installed below slide holder, the light that xenon lamp sends is through the optical density dish, after exciting interference filter, shine on the slide holder again, light intensity detector promptly may detect this light signal, after the integrated operational amplifier amplification, send into computing machine and handle, adjust the exposure of optical density dish or adjustment print again by computer control.

Principle of the present invention is: biological chips detection system can carry out online detection to the light intensity and the homogeneity of xenon lamp exciting light sources by inner light intensity detection system, and by computing machine according to the light intensity value that the is detected control of being correlated with, both can adjust light intensity by the optical density dish, also can adjust the exposure of biological print, to reduce biological chips detection system caused detection error of difference owing to exciting light sources in detection.

The invention has the beneficial effects as follows, has the biological chips detection system that light intensity is surveyed, can detect in time, effectively whether xenon source working properly, whether light intensity even, and can control adjustment by computing machine easily, the required electric current of light intensity probe portion circuit is very little, primary power is not impacted, and circuit only adopts photo-detector and integrated transporting discharging device, resistance, electric capacity etc., and the optical density dish is simple in structure, it is easy to adjust, economical, safe to install, reliably.

Description of drawings

Fig. 1 is a systematic schematic diagram of the present invention.

Embodiment

As shown in Figure 1, the embodiment of the invention is by image acquistion system 1, optical system 2, emission interference filter 3, computing machine 4, slide holder 5, photo-detector 6, integrated transporting discharging 7, xenon source 8, optical density dish 9 and excite interference filter 10 to form.Open xenon lamp power supply, the light that xenon lamp 8 sends is through optical density dish 9, after exciting interference filter 10, shine on the slide holder 5 again, at this moment, the light intensity detector 6 that is installed under the slide holder 5 promptly may detect this light signal, and this signal is after operational amplifier 7 amplifies, flow to computing machine 4, the light intensity data that 4 bases of computing machine receive is in time handled, and adjusts the exposure of optical density dish 9 or adjustment print, to eliminate the error information detection that causes because of the light source intensity variation.During detection, be placed on print on the slide holder 5 after excitation, the target molecule that is marked with fluorescent dye on the print is gone into optics system 2 at the monochromatic fluorescence that produces down of exciting through emission spike interference filter 3 is laggard, optical system 2 is delivered to data acquisition system (DAS) 1 with the picture intelligence of catching, the picture intelligence that data acquisition system (DAS) 1 is caught optical system 2 changes digital signal into delivers to computing machine 4, again the detection data processing of being correlated with by computing machine 4.

Optical density dish 9 is by constituting through the optical device of coating film treatment, its light to see through filter be equally distributed by 99%～5% in 0 °～360 ° scopes.The optical density dish can pass through its rotation of driven by motor, when the light intensity of exciting light sources changes, computing machine can spiral according to the light intensity signal automatic control motor drive optical density that detects and forward the desired position to adjust the incident intensity of exciting light, and computing machine also can change the exposure that being provided with of time shutter adjusted print as required.What the light intensity testing circuit components and parts that added were few, photo-detector 6 adopts is the GT101 photodetector, the light detection sensitivity height, simple in structure, the simple installation of optical density dish, in addition, the required power of testing circuit is very little, influence to biological chips detection system can be ignored, and testing circuit is in low-duty, safety, reliable.

Claims

1, has the biochip test method that light intensity detects in real time, it is characterized in that: in the exciting light light path of biological chips detection system, add an optical density dish, to adjust light intensity, an optical detection system is installed below slide holder, light intensity on the detected print of arrival that detection is sent by xenon source, this light intensity value has truly reflected the truth of excitation source light intensity, this light intensity value record to some extent in computing machine, when this light intensity value change, computer system is judged according to the signal that testing circuit provides, in time take measures, adjust the exposure of optical density dish or adjustment print, to guarantee to detect the true and reliable of data.

2, the biochip test method with the real-time detection of light intensity according to claim 1, it is characterized in that: the exposure of described adjustment optical density dish or adjustment print is meant: when the light intensity of exciting light sources changes, computing machine can spiral according to the light intensity signal automatic control motor drive optical density that detects and forward the desired position to adjust the incident intensity of exciting light, and computing machine also can change the exposure that being provided with of time shutter adjusted print as required.

3, the biochip test method with the real-time detection of light intensity according to claim 1, it is characterized in that: described optical density dish is to be made of the optical device through coating film treatment, and the transmitance of its light is evenly to distribute by 99%～5% in 0 °～360 ° scopes.

4, has the biological chips detection system that light intensity detects in real time, it comprises optical system, the emission interference filter, computing machine, slide holder, xenon source, excite interference filter, it is characterized in that: after xenon source, add the optical density dish that is useful on the adjusting light intensity, photo-detector is installed below slide holder, the light that xenon lamp sends is through the optical density dish, after exciting interference filter, shine on the slide holder again, light intensity detector promptly may detect this light signal, after the integrated operational amplifier amplification, send into computing machine and handle, adjust the exposure of optical density dish or adjustment print again by computer control.

5, the biological chips detection system with the real-time detection of light intensity according to claim 4, it is characterized in that: described photo-detector adopts the GT101 photodetector.