CN100424165C - Process of producing live microbial composition EM prepn - Google Patents
Process of producing live microbial composition EM prepn Download PDFInfo
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- CN100424165C CN100424165C CNB2006100197532A CN200610019753A CN100424165C CN 100424165 C CN100424165 C CN 100424165C CN B2006100197532 A CNB2006100197532 A CN B2006100197532A CN 200610019753 A CN200610019753 A CN 200610019753A CN 100424165 C CN100424165 C CN 100424165C
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- 239000000203 mixture Substances 0.000 title claims abstract description 10
- 230000000813 microbial effect Effects 0.000 title claims abstract description 7
- 238000000034 method Methods 0.000 title description 7
- 235000007164 Oryza sativa Nutrition 0.000 claims abstract description 25
- 235000009566 rice Nutrition 0.000 claims abstract description 25
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims abstract description 25
- 238000005406 washing Methods 0.000 claims abstract description 21
- 241000894006 Bacteria Species 0.000 claims abstract description 19
- 238000000855 fermentation Methods 0.000 claims abstract description 13
- 230000004151 fermentation Effects 0.000 claims abstract description 13
- 238000002156 mixing Methods 0.000 claims abstract description 12
- 238000004519 manufacturing process Methods 0.000 claims abstract description 10
- 238000002360 preparation method Methods 0.000 claims abstract description 9
- 239000002699 waste material Substances 0.000 claims abstract description 8
- 239000007788 liquid Substances 0.000 claims abstract description 7
- 235000013379 molasses Nutrition 0.000 claims abstract description 7
- 239000002002 slurry Substances 0.000 claims abstract description 6
- 229920002472 Starch Polymers 0.000 claims abstract description 4
- 241000209094 Oryza Species 0.000 claims description 24
- 238000003756 stirring Methods 0.000 claims description 17
- 229940041514 candida albicans extract Drugs 0.000 claims description 8
- 239000012138 yeast extract Substances 0.000 claims description 8
- 108090000145 Bacillolysin Proteins 0.000 claims description 7
- 102000035092 Neutral proteases Human genes 0.000 claims description 7
- 108091005507 Neutral proteases Proteins 0.000 claims description 7
- 241000209051 Saccharum Species 0.000 claims description 5
- 239000003795 chemical substances by application Substances 0.000 claims description 5
- 241000196324 Embryophyta Species 0.000 claims description 4
- 235000019890 Amylum Nutrition 0.000 claims description 3
- 159000000003 magnesium salts Chemical class 0.000 claims description 3
- 150000003016 phosphoric acids Chemical class 0.000 claims description 3
- 150000003839 salts Chemical class 0.000 claims description 3
- FAPWRFPIFSIZLT-UHFFFAOYSA-M Sodium chloride Chemical compound [Na+].[Cl-] FAPWRFPIFSIZLT-UHFFFAOYSA-M 0.000 abstract description 11
- 235000002639 sodium chloride Nutrition 0.000 abstract description 8
- 230000001580 bacterial effect Effects 0.000 abstract description 6
- 239000011780 sodium chloride Substances 0.000 abstract description 6
- 244000144972 livestock Species 0.000 abstract description 3
- 240000007594 Oryza sativa Species 0.000 abstract 1
- 229910019142 PO4 Inorganic materials 0.000 abstract 1
- 240000004808 Saccharomyces cerevisiae Species 0.000 abstract 1
- 101000693530 Staphylococcus aureus Staphylokinase Proteins 0.000 abstract 1
- QAOWNCQODCNURD-UHFFFAOYSA-L Sulfate Chemical compound [O-]S([O-])(=O)=O QAOWNCQODCNURD-UHFFFAOYSA-L 0.000 abstract 1
- 238000009395 breeding Methods 0.000 abstract 1
- 230000001488 breeding effect Effects 0.000 abstract 1
- 238000001816 cooling Methods 0.000 abstract 1
- 235000013305 food Nutrition 0.000 abstract 1
- 238000010438 heat treatment Methods 0.000 abstract 1
- 238000009776 industrial production Methods 0.000 abstract 1
- NBIIXXVUZAFLBC-UHFFFAOYSA-K phosphate Chemical compound [O-]P([O-])([O-])=O NBIIXXVUZAFLBC-UHFFFAOYSA-K 0.000 abstract 1
- 239000010452 phosphate Substances 0.000 abstract 1
- 230000001105 regulatory effect Effects 0.000 abstract 1
- 235000019698 starch Nutrition 0.000 abstract 1
- 239000008107 starch Substances 0.000 abstract 1
- 239000001963 growth medium Substances 0.000 description 5
- 244000005700 microbiome Species 0.000 description 5
- 239000000047 product Substances 0.000 description 5
- 239000011734 sodium Substances 0.000 description 5
- 239000002253 acid Substances 0.000 description 4
- 235000009508 confectionery Nutrition 0.000 description 4
- 230000000694 effects Effects 0.000 description 4
- 238000011081 inoculation Methods 0.000 description 4
- 238000009413 insulation Methods 0.000 description 4
- 235000015097 nutrients Nutrition 0.000 description 4
- 102000004190 Enzymes Human genes 0.000 description 3
- 108090000790 Enzymes Proteins 0.000 description 3
- 230000000050 nutritive effect Effects 0.000 description 3
- 239000002994 raw material Substances 0.000 description 3
- OAICVXFJPJFONN-UHFFFAOYSA-N Phosphorus Chemical compound [P] OAICVXFJPJFONN-UHFFFAOYSA-N 0.000 description 2
- ZLMJMSJWJFRBEC-UHFFFAOYSA-N Potassium Chemical compound [K] ZLMJMSJWJFRBEC-UHFFFAOYSA-N 0.000 description 2
- UIIMBOGNXHQVGW-UHFFFAOYSA-M Sodium bicarbonate Chemical compound [Na+].OC([O-])=O UIIMBOGNXHQVGW-UHFFFAOYSA-M 0.000 description 2
- 201000010099 disease Diseases 0.000 description 2
- 208000037265 diseases, disorders, signs and symptoms Diseases 0.000 description 2
- 238000005516 engineering process Methods 0.000 description 2
- 230000002708 enhancing effect Effects 0.000 description 2
- DNJIEGIFACGWOD-UHFFFAOYSA-N ethanethiol Chemical compound CCS DNJIEGIFACGWOD-UHFFFAOYSA-N 0.000 description 2
- 230000003053 immunization Effects 0.000 description 2
- JVTAAEKCZFNVCJ-UHFFFAOYSA-N lactic acid Chemical compound CC(O)C(O)=O JVTAAEKCZFNVCJ-UHFFFAOYSA-N 0.000 description 2
- 238000005360 mashing Methods 0.000 description 2
- 235000016709 nutrition Nutrition 0.000 description 2
- 230000035764 nutrition Effects 0.000 description 2
- 239000011574 phosphorus Substances 0.000 description 2
- 229910052698 phosphorus Inorganic materials 0.000 description 2
- 230000008635 plant growth Effects 0.000 description 2
- 239000004033 plastic Substances 0.000 description 2
- 229920003023 plastic Polymers 0.000 description 2
- 239000011591 potassium Substances 0.000 description 2
- 229910052700 potassium Inorganic materials 0.000 description 2
- 244000144977 poultry Species 0.000 description 2
- 230000001737 promoting effect Effects 0.000 description 2
- 239000002689 soil Substances 0.000 description 2
- 239000000126 substance Substances 0.000 description 2
- 241000186361 Actinobacteria <class> Species 0.000 description 1
- 241000233866 Fungi Species 0.000 description 1
- WQZGKKKJIJFFOK-GASJEMHNSA-N Glucose Natural products OC[C@H]1OC(O)[C@H](O)[C@@H](O)[C@@H]1O WQZGKKKJIJFFOK-GASJEMHNSA-N 0.000 description 1
- FYYHWMGAXLPEAU-UHFFFAOYSA-N Magnesium Chemical compound [Mg] FYYHWMGAXLPEAU-UHFFFAOYSA-N 0.000 description 1
- 244000061456 Solanum tuberosum Species 0.000 description 1
- 235000002595 Solanum tuberosum Nutrition 0.000 description 1
- CZMRCDWAGMRECN-UGDNZRGBSA-N Sucrose Chemical compound O[C@H]1[C@H](O)[C@@H](CO)O[C@@]1(CO)O[C@@H]1[C@H](O)[C@@H](O)[C@H](O)[C@@H](CO)O1 CZMRCDWAGMRECN-UGDNZRGBSA-N 0.000 description 1
- 229930006000 Sucrose Natural products 0.000 description 1
- 239000005864 Sulphur Substances 0.000 description 1
- 229930003270 Vitamin B Chemical group 0.000 description 1
- 238000009360 aquaculture Methods 0.000 description 1
- 244000144974 aquaculture Species 0.000 description 1
- WQZGKKKJIJFFOK-VFUOTHLCSA-N beta-D-glucose Chemical compound OC[C@H]1O[C@@H](O)[C@H](O)[C@@H](O)[C@@H]1O WQZGKKKJIJFFOK-VFUOTHLCSA-N 0.000 description 1
- 150000001720 carbohydrates Chemical group 0.000 description 1
- 235000014633 carbohydrates Nutrition 0.000 description 1
- 239000002131 composite material Substances 0.000 description 1
- 239000012531 culture fluid Substances 0.000 description 1
- 238000011161 development Methods 0.000 description 1
- 238000003912 environmental pollution Methods 0.000 description 1
- 239000006052 feed supplement Substances 0.000 description 1
- 235000011389 fruit/vegetable juice Nutrition 0.000 description 1
- 239000008103 glucose Substances 0.000 description 1
- 235000001727 glucose Nutrition 0.000 description 1
- 230000012010 growth Effects 0.000 description 1
- 235000012907 honey Nutrition 0.000 description 1
- 239000012535 impurity Substances 0.000 description 1
- 239000004615 ingredient Substances 0.000 description 1
- 229910017053 inorganic salt Inorganic materials 0.000 description 1
- 235000014655 lactic acid Nutrition 0.000 description 1
- 239000011777 magnesium Substances 0.000 description 1
- 229910052749 magnesium Inorganic materials 0.000 description 1
- 238000011177 media preparation Methods 0.000 description 1
- 231100000957 no side effect Toxicity 0.000 description 1
- 230000000243 photosynthetic effect Effects 0.000 description 1
- 238000002203 pretreatment Methods 0.000 description 1
- 239000006041 probiotic Substances 0.000 description 1
- 235000018291 probiotics Nutrition 0.000 description 1
- 235000018102 proteins Nutrition 0.000 description 1
- 108090000623 proteins and genes Chemical group 0.000 description 1
- 102000004169 proteins and genes Human genes 0.000 description 1
- 239000000718 radiation-protective agent Substances 0.000 description 1
- 238000012827 research and development Methods 0.000 description 1
- 235000017557 sodium bicarbonate Nutrition 0.000 description 1
- 229910000030 sodium bicarbonate Inorganic materials 0.000 description 1
- 239000005720 sucrose Substances 0.000 description 1
- 239000011573 trace mineral Substances 0.000 description 1
- 235000013619 trace mineral Nutrition 0.000 description 1
- 239000011720 vitamin B Chemical group 0.000 description 1
- 235000019156 vitamin B Nutrition 0.000 description 1
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- Cereal-Derived Products (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
- Fodder In General (AREA)
Abstract
The live microbial composition EM preparation is produced with rice washing water and through regulating pH value, treating with neutral proteinase, adding certain amount of one of waste molasses, red sugar and starch saccarifying slurry, phosphate, table salt, sulfate and yeast paste, mixing homogeneously, heating at 100 deg.c for 5 min, cooling, inoculating live EM bacterial liquid in 10-20 %, and fermentation to culture. The present invention has simple production process suitable for industrial production and low cost, and can produce high quality live microbial composition EM preparation with high live bacteria content for use in farm production, livestock breeding, food processing, environment protection and other fields.
Description
Technical field
The present invention relates to a kind of production method of live microbial composition EM preparation.
Background technology
EM is the english abbreviation of effective microorganism (efective microorganisms), is present more common a kind of probiotics.Mainly belonging to 80 kinds of microorganisms by photosynthetic bacterium, milk-acid bacteria, actinomycetes, fermented type thread fungus etc. 10 and form, is the special flora that a kind of anaerobism and oxytropism have both at the same time.It at first is that Japanese Ryukyu university studies successful a kind of NEW TYPE OF COMPOSITE microorganism live bacteria preparation than good according to people such as professors husband.EM does not contain any chemical objectionable impurities, has no side effect, and noresidue and secondary pollution do not produce antiradiation drug.Since coming out the eighties in 20th century, promote the use of in more than 60 countries and regions such as Japan, Brazil, the U.S., Thailand, all show good effect at aspects such as promoting plant-growth, enhancing livestock and poultry resistance against diseases, the immunizing power that strengthens the breed object, removal ight soil stench, improve the ecological environment and improve the quality of products.EM introduces China the beginning of the nineties, promote through these years experimental study, has obtained significant effect in fields such as non-polluted planting industry, aquaculture and environment protection, and the Sustainable development of agricultural has been produced huge pushing effect.
After EM introduced China, through relevant scientific and technical personnel's continuous exploration, research and development, its culture technique was grasped substantially, and culture medium prescription presents variation.A kind of preparation method of nutrient solution of the EM of being applicable to viable bacteria production is disclosed as Chinese patent application " preparation method of the nutrient solution that a kind of EM of being applicable to viable bacteria produces " (application number 02145041.2), this method is that raw material is made juice with the potato, adding relatively large sucrose, glucose, honey, sodium bicarbonate then mixes and stirs evenly, make after the nutrient solution, add the EM viable bacteria by 1: 1 volume ratio, be greater than or less than the cultivation of room temperature bottom fermentation.This culture medium prescription, the raw material that is adopted is more expensive, the cost height, nutrition is owed comprehensively comparatively speaking, does not add as micro-sulphur, phosphorus, potassium, magnesium etc., will certainly have influence on the growth of EM viable bacteria like this, thereby have influence on the quality of product.
Summary of the invention
The technical problem to be solved in the present invention provides that a kind of manufacturing process is simple, culture medium cost is low, the production method of the comprehensive EM live bacteria agent of nutritive substance.
The present invention solves the problems of the technologies described above with following technical scheme:
A kind of production method of live microbial composition EM preparation, utilize rice washing water to cultivate the active liquid of EM, after it is characterized in that the rice washing water pH value transferred to neutrality, (as follows) is the ratio adding neutral protease of 0.001%-0.01% by weight percentage, be heated to 40～50 ℃, be incubated 1 hour, stirred once every 10 minutes; A kind of and phosphoric acid salt, magnesium salts, salt, yeast extract paste, mixing and stirring to adding by a certain percentage through pretreated rice washing water in waste molasses or brown sugar (Saccharum Sinensis Roxb.) or the plant amylum saccharification slurries are heated to 100 ℃, 5 minutes; To be cooled to 35-40 ℃, the EM of 10～20% ratio adding by weight percentage strain liquid mixing secondary fermentation promptly gets live bacteria agent then.
Contain abundant carbohydrate, protein, vitamin B group, phosphorus, potassium and various trace element in the rice washing water, be of high nutritive value, after the EM viable bacteria fermentation transforms, nutritive value and result of use further improve, and all show better effect at aspects such as promoting plant-growth, enhancing livestock and poultry resistance against diseases, the immunizing power that strengthens the breed object, removal ight soil stench, improve the ecological environment and improve the quality of products.And use method of the present invention to produce the EM live bacteria agent, simple and reliable process, with low cost, suitable suitability for industrialized production.
Embodiment
With the major ingredient of rice washing water (also claiming washing water of rice) as bacteria culture fluid, utilize in the rice washing water unique nutrition and microorganism species efficiently, add feed supplements such as small amounts of inorganic salt, waste molasses, can produce high-quality EM living bacterial liquid, not only product performance are superior, can also reduce the plant effuent quantity discharged, reduce environmental pollution, reach the purpose that turns waste into wealth.
Method technological process of the present invention is as follows:
1, the pre-treatment of rice washing water:
Rice washing water can be used (Na)
2CO
3Solution transfers to about PH7.0, adds neutral protease in the 0.001%-0.01% ratio then, is heated to 40～50 ℃, is incubated 1 hour, stirs once every 10 minutes.
2, culture medium preparation and fermentation:
The pretreated rice washing water of learning from else's experience adds a kind of and phosphoric acid salt, magnesium salts, salt, yeast extract paste, mixing and stirring in waste molasses or plant amylum saccharification slurries or the brown sugar (Saccharum Sinensis Roxb.) by a certain percentage, is heated to 100 ℃, 5 minutes.To be cooled to 40 ℃, 10～20% ratio adds the mixing of EM strain liquid by weight percentage, places plastic seal container or acidproof fermentor tank, and fermentation promptly gets the live bacteria agent product.Above-mentioned culture medium prescription can be: add useless sugared close 1-3% or mashing slurries 8-10% or brown sugar (Saccharum Sinensis Roxb.) 1-2% in the rice washing water, NaCl 0.1-0.3%, KH
2PO
40.2-0.3%, MgSO
40.01-0.03%, yeast extract 0.1-0.3%.
Above-mentioned leavening temperature is between 20～37 ℃, and the length of fermentation time is decided according to the temperature of fermentation.
Can also adopt with the rice washing water is that main raw material is made various types of rice washing water nutrient solutions.
Embodiment 1
Get rice: the slubbing rice washing water 1000g of wash water=1: 1 (weight ratio), use Na
2CO
3After solution transfers to PH7.0, add neutral protease 0.1g (0.01%), stir, be heated to 40 ℃ of insulations and handled 1 hour, middle every stirring in 10 minutes 1 time.Add NaCl 1g (0.1%) then, waste molasses 20g (2%), KH
2PO
41g (0.1%), MgSO
40.2g (0.02%), yeast extract 1g (0.1%), mixing and stirring was boiled 5 minutes, and is to be cooled to 35 ℃, inoculation EM viable bacteria 150g (15%), mixing is put in the plastics pot and is sealed, and 24～28 ℃ of leavening temperatures, fermentation time are 96 hours.PH is 3.5, and bacterial count reaches 3.6 * 10
9Individual/ml, smell acid is with fragrant and sweet.
Embodiment 2
Get rice: the slubbing rice washing water 500kg of wash water=1: 2 (weight ratio) adds an amount of Na
2CO
3After solution transfers to PH7.0, add neutral protease 50g (0.01%), stir, heat 45 ℃ of insulation enzymes and handled 1 hour, middle every stirring in 10 minutes 1 time.Add NaCl 1kg (0.2%) then, waste molasses 10kg (2%), KH
2PO
41kg (0.2%), MgSO
40.075kg (0.015%), yeast extract 1.0kg (0.2%), mixing and stirring, heat 100 ℃ 5 minutes, to be cooled to 35～40 ℃, the inoculation EM viable bacteria 50kg (10%), mix, leavening temperature is 33 ℃, fermentation time is 85 hours.PH is 3.5, and bacterial count reaches 3.7 * 10
9Individual/ml, smell acid is with fragrant and sweet.
Embodiment 3
Get rice: the slubbing rice washing water 500kg of wash water=1: 2.5 (weight ratio) adds an amount of Na
2CO
3After solution transfers to PH7.0, add neutral protease 20g (0.004%), stir, heat 45 ℃ of insulation enzymes and handled 1 hour, middle every stirring in 10 minutes 1 time.Add NaCl 1.5kg (0.3%) then, mashing slurries 45kg (9%), KH
2PO
41.5kg MgSO (0.3%),
40.075kg (0.015%), yeast extract 1kg (0.2%), mixing and stirring, heat 100 ℃ 5 minutes, to be cooled to 35～40 ℃, the inoculation EM viable bacteria 50kg (10%), mix, leavening temperature is 35 ℃, fermentation time is 85 hours.PH is 3.5, and bacterial count reaches 3.7 * 10
9Individual/ml, smell acid is with fragrant and sweet.
Embodiment 4
Get rice: the slubbing rice washing water 500kg of wash water=1: 2.8 (weight ratio) adds an amount of Na
2CO
3After solution transfers to PH7.0, add neutral protease 5g (0.001%), stir, heat 45 ℃ of insulation enzymes and handled 1 hour, middle every stirring in 10 minutes 1 time.Add NaCl 0.5kg (0.1%) then, brown sugar (Saccharum Sinensis Roxb.) 5kg (1%), KH
2PO
40.5kg MgSO (0.1%),
40.15kg (0.03%), yeast extract 1.5kg (0.3%), mixing and stirring, heat 100 ℃ 5 minutes, to be cooled to 35～40 ℃, the inoculation EM viable bacteria 100kg (20%), mix, leavening temperature is 36 ℃, fermentation time is 72 hours.PH is 3.4, and bacterial count reaches 4.6 * 10
9Individual/ml, smell acid is with fragrant and sweet.
Claims (1)
1. the production method of a live microbial composition EM preparation, utilize rice washing water to cultivate the active liquid of EM, after it is characterized in that the rice washing water pH value transferred to neutrality, the ratio that is 0.001%-0.01% by weight percentage adds neutral protease, be heated to 40～50 ℃, be incubated 1 hour, stirred once every 10 minutes; To a kind of and phosphoric acid salt, magnesium salts, salt and the yeast extract paste mixing and stirring that add by a certain percentage through pretreated rice washing water in waste molasses or brown sugar (Saccharum Sinensis Roxb.) or the plant amylum saccharification slurries; Be heated to 100 ℃, 5 minutes; To be cooled after 35-40 ℃, the EM of 10～20% ratio adding by weight percentage strain liquid mixing secondary fermentation promptly gets live bacteria agent.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CNB2006100197532A CN100424165C (en) | 2006-07-27 | 2006-07-27 | Process of producing live microbial composition EM prepn |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CNB2006100197532A CN100424165C (en) | 2006-07-27 | 2006-07-27 | Process of producing live microbial composition EM prepn |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN1888046A CN1888046A (en) | 2007-01-03 |
| CN100424165C true CN100424165C (en) | 2008-10-08 |
Family
ID=37577331
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CNB2006100197532A Active CN100424165C (en) | 2006-07-27 | 2006-07-27 | Process of producing live microbial composition EM prepn |
Country Status (1)
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|---|---|
| CN (1) | CN100424165C (en) |
Families Citing this family (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101591631B (en) * | 2009-06-08 | 2011-04-13 | 江西省天意生物技术开发有限公司 | EM original dew and production method thereof |
| CN103255059A (en) * | 2012-02-15 | 2013-08-21 | 何寒 | Preparation method of high purity EM protospecies |
| CN103255074B (en) * | 2012-02-15 | 2014-12-03 | 何寒 | Simple and rapid propagation method of EM |
| CN102911903A (en) * | 2012-10-29 | 2013-02-06 | 丘佩珍 | Cultivation of lactic acid bacteria and method of keeping nuisance-free rabbits with lactic acid bacteria |
| CN104531802A (en) * | 2014-12-11 | 2015-04-22 | 无锡市善源生物科技有限公司 | Method for preparing edible bacterial cellulose by using rice immersing water and product of method |
| CN112553010A (en) * | 2020-12-17 | 2021-03-26 | 聂绪明 | EM (effective microorganisms) bacterium laundry detergent and preparation method thereof |
Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US5246848A (en) * | 1991-05-20 | 1993-09-21 | Olin Corporation | Process for providing enhanced yield and simplified isolation of hydrophobic enzymes from culture media |
| JP2002080288A (en) * | 2000-06-30 | 2002-03-19 | Asake Seiko Kk | Treatment method of garbage |
| CN1498961A (en) * | 2002-11-05 | 2004-05-26 | 张德怀 | Method for preparing culture fluid suitable to production of EM live bacterium |
-
2006
- 2006-07-27 CN CNB2006100197532A patent/CN100424165C/en active Active
Patent Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US5246848A (en) * | 1991-05-20 | 1993-09-21 | Olin Corporation | Process for providing enhanced yield and simplified isolation of hydrophobic enzymes from culture media |
| JP2002080288A (en) * | 2000-06-30 | 2002-03-19 | Asake Seiko Kk | Treatment method of garbage |
| CN1498961A (en) * | 2002-11-05 | 2004-05-26 | 张德怀 | Method for preparing culture fluid suitable to production of EM live bacterium |
Non-Patent Citations (4)
| Title |
|---|
| 利用淘米水培养EM活性液的方法以及应用技术. 崔春兰等.安徽农学通报，第11卷第6期. 2005 |
| 利用淘米水培养EM活性液的方法以及应用技术. 崔春兰等.安徽农学通报，第11卷第6期. 2005 * |
| 新型EM菌剂的研制. 詹益全等.家畜生态，第22卷第3期. 2001 |
| 新型EM菌剂的研制. 詹益全等.家畜生态，第22卷第3期. 2001 * |
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|---|---|
| CN1888046A (en) | 2007-01-03 |
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Effective date of registration: 20170714 Address after: 518052 Guangdong city of Shenzhen province Qianhai Shenzhen Hong Kong cooperation zone before Bay Road No. 1 building 201 room A (located in Shenzhen Qianhai business secretary Co. Ltd.) Patentee after: Zhongjian Biotechnology (Shenzhen) Co., Ltd. Address before: Nanning City People's Road West 530012 No. 6 the Guangxi Zhuang Autonomous Region second kindergarten King turn red Co-patentee before: Zhang Xuerong Patentee before: Zhou Malin |