CN100404525C - Process of extracting dihydromyricitrin from vine tea - Google Patents
Process of extracting dihydromyricitrin from vine tea Download PDFInfo
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- CN100404525C CN100404525C CNB2005100329185A CN200510032918A CN100404525C CN 100404525 C CN100404525 C CN 100404525C CN B2005100329185 A CNB2005100329185 A CN B2005100329185A CN 200510032918 A CN200510032918 A CN 200510032918A CN 100404525 C CN100404525 C CN 100404525C
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Abstract
The present invention discloses a method for extracting dibydromyricetrin from vine tea, which comprises: dry leaves of the vine tea are soaked in a solvent and filtered and the wet leaves of the vine tea are treated by microwave radiation; filtered solutions are combined and heated in water bath to extract the dibydromyricetrin; the hot filtered solutions are filtered, dissolved with hot water, and separated and purified by a cold water recrystallization method. The extracting method of the present invention has the advantages of high extraction rate, simple and convenient operation and few by-products. The method for extracting dibydromyricetrin from vine tea is a good method for extracting dihydromyricetin.
Description
Technical field
The present invention relates to biochemistry and modernization of Chinese medicine technical field, relate in particular to a kind of method of from vine tea, extracting dibydro myricetrin.
Background technology
Vine tea is a kind of very ancient natural resources of Chinese medicinal materials, class tea plant resources and medicine food dual purpose plant resource.Vine tea is all on the books in the successive dynasties flora and in the medicinal plant register, and vine tea is a kind of time-honored plant, and is the same with other herbal medicine, in human and the Nature are struggled process, once plays a part important.Even to this day, along with the deepening continuously and develop of human knowledge's nature, along with improving constantly of human research's level, vine tea will become human a kind of important plant resources.
" vine tea (claiming wild vine tea, white tea, white tea cake again); belong to Vitaceae; Ampelopsis; formal name used at school is Ampelopsis grossedentata (Ampelopsis grossedentata); be a kind of perennial vine; mainly be distributed in provinces and regions such as Guangdong, Guangxi, Hainan, Yunnan, Guizhou, Hunan, Hubei, Jiangxi, Fujian, be grown in the hillside, woods of height above sea level 400～1300m, river shoal etc. is dark and damp and fertile soil in, it is a kind of wild natural class tea plant alternative tea with unique health-care effect and consumption value, complete stool is medicinal, and it is sweet, light, cool in nature to distinguish the flavor of.Have effects such as clearing heat and detoxicating, wind-damp dispelling, strengthening the bones and muscles, among the people its young stem and leaf is made health tea, be used for the treatment of diseases such as cold, fever, swelling and pain in the throat, icterohepatitis, blister furuncle, the use history in existing hundreds of years.The mountain area common people of Jiang Hua, Hunan county Yao nationality gather cauline leaf, through kneading, drying, are used for anti-curing cold, heatstroke, gastrointestinal upset etc., pass on from one to another existing hundreds of years applicating histories." herbal medicine compilation " and " Guangxi medicinal plant register " record, this property of medicine slightly sweet flavor, effect is clearing heat and detoxicating, cures mainly icterohepatitis, cold anemopyretic, swelling and pain in the throat, also has antialcoholism action.
Main effective constituent is flavonoid compound in the vine tea, and with the dihydromyricetin cellulose content for the highest, be the highest plant of body burden greatly in the flavanone kind composition of finding both at home and abroad at present.Show that through pharmacological evaluation dibydro myricetrin has obvious antagonism and high K
+Due to rabbit thoracic aorta bar contractile response and calcium antagonism, and toxicity is low, can be used as anti-arrhythmia, myocardial ischemia, hypertensive newtype drug, can eliminate the phlegm, anti-inflammatory, cough-relieving, lipopenicillinase, liver protecting; Remove alcoholism; Suppress the formation of extracorporeal platelet aggregation and thrombus in vivo, blood fat reducing and glucose level, SOD activity improving is expected to be developed as one, two national kind new medicines.The bagged steeping drug that this medicinal material is made is used for hypertension clinically and the coronary heart disease assisting therapy has obtained positive effect.
In view of the vital role of flavonoid compound dibydro myricetrin in the vine tea leaf, the dibydro myricetrin that fully develops in the vine tea leaf is of great practical significance.At present, the extraction of dibydro myricetrin mainly is to come from ampelopsis, and its extraction process is loaded down with trivial details, and extraction yield is low, has only about 20%.
Summary of the invention
The objective of the invention is to overcome above-mentioned dibydro myricetrin and extract the problem that exists, provide a kind of easy and simple to handle, by product is few, the extracting method of the dibydro myricetrin that productive rate is high.
Microwave technology is used more and more widely in people's productive life, and it has characteristics such as reaction high efficiency and strong selectivity in leaching process, and easy and simple to handle, and by product is few, advantages such as the easy purification of productive rate height and product.The principle of microwave technology is to utilize the quick vibrations of 24.5 hundred million ultra high frequencies of per second that magnetron produces, and makes intermolecular mutual collision in the vine tea, extruding, helps the leaching of effective constituent like this.
The method of extracting dibydro myricetrin from vine tea of the present invention specifically comprises the steps:
(1) the vine tea cured leaf is soaked in solvent;
(2) filter, the vine tea leaf that will wet carries out microwave radiation processing;
(3) the vine tea leaf of filtrate with the process microwave radiation merged, heating in water bath extracts;
(4) filtered while hot, the filtrate Crystallization Separation.
The described solvent of above-mentioned steps (1) is water or ethanol; The solid-liquid ratio of above-mentioned vine tea cured leaf and solvent is 1: 12～20g/ml, and best solid-liquid ratio is 1: 20g/ml, optimum solvent is a water.
The time of the described microwave treatment of above-mentioned steps (2) is 1～3 minute; The time of best microwave treatment is 3 minutes, and best microwave power is high-grade, i.e. 900W.
The time of the described heating in water bath of above-mentioned steps (3) is 30～120 minutes, and bath temperature is 60～90 ℃; The time of best heating in water bath is 120 minutes.
Beneficial effect of the present invention: the present invention adopts microwave radiation processing vine tea leaf, utilize the quick vibrations of 24.5 hundred million ultra high frequencies of per second that magnetron produces, make intermolecular mutual collision in the vine tea, extruding, be beneficial to the abundant leaching of effective constituent dibydro myricetrin, thereby improved the extraction yield of dibydro myricetrin, reached 29%.In addition, the present invention is easy and simple to handle, and by product is few, is a kind of good method of extracting dibydro myricetrin.
Embodiment
Embodiment 1
Take by weighing vine tea cured leaf 10g, soak 10min with the water yield of 200ml, the tealeaves that will wet after the filtration carries out microwave radiation processing 3min with microwave oven (NN-K542MF), and microwave power is 900W, merging filtrate, and heating in water bath extracts 120min in 80 ℃ thermostat water bath.Filtered while hot, the filtrate crystallization obtains pure dibydro myricetrin, and extraction yield is 29%.
Claims (7)
1. method of extracting dibydro myricetrin from vine tea is characterized in that adopting the method for microwave radiation processing, comprises the steps:
(1) the vine tea cured leaf is soaked in solvent;
(2) filter, the vine tea leaf that will wet carries out microwave radiation processing, and the time of microwave treatment is 1～3 minute;
(3) the vine tea leaf of filtrate with the process microwave radiation merged, heating in water bath extracts, and the time of heating in water bath is 30～120 minutes, and bath temperature is 60～90 ℃;
(4) filtered while hot, the filtrate Crystallization Separation.
2. the method for extracting dibydro myricetrin from vine tea as claimed in claim 1 is characterized in that the described solvent of step (1) is water or ethanol.
3. the method for extracting dibydro myricetrin from vine tea as claimed in claim 2 is characterized in that the described solvent of step (1) is a water.
4. as claim 1 or the 2 or 3 described methods of from vine tea, extracting dibydro myricetrin, it is characterized in that the solid-liquid ratio of described vine tea cured leaf of step (1) and solvent is 1: 12～20g/ml.
5. the method for extracting dibydro myricetrin from vine tea as claimed in claim 4, it is characterized in that the solid-liquid ratio of described vine tea cured leaf of step (1) and solvent is 1: 20g/ml, solvent are water.
6. the method for extracting dibydro myricetrin from vine tea as claimed in claim 1 is characterized in that the time of the described microwave treatment of step (2) is 3 minutes, and microwave power is 900W.
7. the method for extracting dibydro myricetrin from vine tea as claimed in claim 1 is characterized in that the time of the described heating in water bath of step (3) is 120 minutes.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CNB2005100329185A CN100404525C (en) | 2005-01-27 | 2005-01-27 | Process of extracting dihydromyricitrin from vine tea |
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| CNB2005100329185A CN100404525C (en) | 2005-01-27 | 2005-01-27 | Process of extracting dihydromyricitrin from vine tea |
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| CN1810798A CN1810798A (en) | 2006-08-02 |
| CN100404525C true CN100404525C (en) | 2008-07-23 |
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| CNB2005100329185A Expired - Fee Related CN100404525C (en) | 2005-01-27 | 2005-01-27 | Process of extracting dihydromyricitrin from vine tea |
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Families Citing this family (7)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101805321B (en) * | 2010-04-16 | 2012-08-22 | 安徽省瑞森生物科技有限责任公司 | Method for extracting dihydromyricetin from vine tea |
| CN101973976B (en) * | 2010-09-16 | 2012-01-18 | 湖北民族学院 | Method for extracting dihydromyricetin, ampelopsis grossedentata amylase and ampelopsis grossedentata polyphenol from ampelopsis grossedentata |
| CN102796069B (en) * | 2012-07-04 | 2015-01-21 | 贵州省生物研究所 | Method for extracting dihydromyricetin and Ampelopsis grossedentata polysaccharide from Ampelopsis grossedentata |
| CN109180629A (en) * | 2018-10-12 | 2019-01-11 | 浙江省林业科学研究院 | A kind of green extraction method improving dihydromyricetin yield |
| CN110810693A (en) * | 2019-10-14 | 2020-02-21 | 北京逸飞时代生物科技有限公司 | A beverage composition containing Ampelopsis grossedentata and its preparation method |
| CN111303107B (en) * | 2020-03-16 | 2021-12-24 | 重庆骄王天然产物股份有限公司 | Method for extracting dihydromyricetin from vine tea |
| CN114223762A (en) * | 2021-11-04 | 2022-03-25 | 宁波大学 | Racemization protection method of dihydromyricetin |
Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1116293C (en) * | 1999-09-16 | 2003-07-30 | 宋新荣 | Preparation and application of dihydromyricetrin |
| CN1712402A (en) * | 2004-06-25 | 2005-12-28 | 广东省农业科学院蚕业与农产品加工研究所 | Extraction of dihydromyricetin from microwave and composition thereof |
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Patent Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1116293C (en) * | 1999-09-16 | 2003-07-30 | 宋新荣 | Preparation and application of dihydromyricetrin |
| CN1712402A (en) * | 2004-06-25 | 2005-12-28 | 广东省农业科学院蚕业与农产品加工研究所 | Extraction of dihydromyricetin from microwave and composition thereof |
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| 二氢杨梅素的稳定性及其影响因素. 林淑英等.无锡轻工大学学报，第23卷第2期. 2004 |
| 二氢杨梅素的稳定性及其影响因素. 林淑英等.无锡轻工大学学报，第23卷第2期. 2004 * |
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Assignee: Daqian Hi-New Tech Research Center Co., Ltd., Shantou City Assignor: Guangzhou University Contract fulfillment period: 2008.12.2 to 2014.7.1 contract change Contract record no.: 2009440001484 Denomination of invention: Method for extracting dihydromyricetin from vine tea Granted publication date: 20080723 License type: Exclusive license Record date: 2009.10.9 |
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Free format text: EXCLUSIVE LICENSE; TIME LIMIT OF IMPLEMENTING CONTACT: 2008.12.2 TO 2014.7.1; CHANGE OF CONTRACT Name of requester: SHANTOU CITY DAQIAN HIGH AND NEW TECHNOLOGY RESEAR Effective date: 20091009 |
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