CN100403909C - Production method of induced anti-heterogenous fungus protein agricultural chemical for promoting crop growth based on moss slag - Google Patents
Production method of induced anti-heterogenous fungus protein agricultural chemical for promoting crop growth based on moss slag Download PDFInfo
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Abstract
The present invention discloses a production method of biological pesticides, more specifically a production method of induced anti-heterogeneous fungus protein agricultural chemicals for promoting crop growth based on marsh gas slag, which is characterized in that the present invention comprises the following steps of 1) solid or liquid culture of CL and/or AA strain seed bacteria, 2) preparation of a marsh gas slag culture medium and fermentation cultivation, and 3) thoroughly mixing, drying and mechanically crushing culture including culture medium matter, fungi and assistant agents which are fermented and cultivated in step 2 to obtain a heterogeneous fungus protein agricultural chemical for crops. The production method of induced anti-heterogeneous fungus protein agricultural chemicals for promoting crop growth based on marsh gas slag can prepare broad-spectrum fungus protein agricultural chemicals as both pesticides and fertilizers for promoting the growth of crops and improving the reverse immunity resistance of the crops.
Description
Technical field
The present invention relates to a kind of production method of biopesticide, be specially production method based on the induced anti-heterogenous fungus protein agricultural chemical for promoting crop growth of natural pond slag.
Background technology
The requirement of 21 century to the used preparation of crops is: effective, consumption is few, inexpensive; Safety non-toxic is harmless; Function is many, purposes is wide; Do not cause that pest produces resistance and induces the resistance of crop enhancing to adverse circumstance and pest; Can improve the green product of the microecosystem of plant and soil.With natural pond fertilizer is the microprotein of matrix and the novel biopesticide that preparation meets this requirement beyond doubt thereof, will receive increasing concern.Biogas is that China solves rural energy resources, improves the rural health environment, improves rich people's engineering of life of farmers quality and income.According to statistics, the farmers' drought lavatory that existing 100,000,000 many places, the whole nation are simple and crude, more than 20 hundred million tons of ight soil of the annual generation of livestock and poultry breeding industry.Natural pond fertilizer (comprising natural pond slag and natural pond liquid) is that people, animal poultry manure, house refuse etc. are carried out anaerobic fermentation, the residue behind the generation biogas.China has family small-sized methane pond (average external volume 8m at present
3) about more than 1,000 ten thousand, there is more than 30,000 large methane tank (jar) in the whole nation, total capacity surpasses 1,500,000 m
3In recent years country and each province (district, city) attaches great importance to rural methane facilities development, and central authorities are annual for successive years to arrange 1,000,000,000 yuan of national debt funds to be used for production and use of marsh gas engineering, and more than 6000 village that project relates to 540 counties of 24 provinces (city, district) benefits 1,030,000 peasant households.Expecting national rural novel biogas generating pit total amount in 2005 will be above 2,000 ten thousand family, will reach 5,000 ten thousand families in 2010.A normal domestic use biogas generating pit can produce fertile about 20 tons of natural pond every year, estimates about 2.5～3.0 hundred million tons of the present whole nation year output natural pond fertilizer.
The natural pond fertilizer that the people, animal poultry manure produces after biogas generating pit (jar) fermentation outside the decarburize loss is big, still keeps 90% above material nutrient component.Nitrogen is fertile high by 40%～60% than the stack retting of the same amount of homogeneity, and its structure is optimized; The rate of recovery of phosphorus element and potassium element is up to 80%～90%.Natural pond fertilizer also contains materials such as multiple micronutrient element, amino acid, hormone and vitamin except containing grand nutrition element, organic matter, be speed, slow having both, plyability, polyphenic fertilizer, and utilizing properly turns waste into wealth; If handle bad then can become one of root that rural environmental pollution, sanitary condition worsen once more.Table 1 has been listed various fertilizer component contents.
Nitrogen, phosphorus, potassium content in the table 1 different fertilizer organic matter
Natural pond fertilizer is the abundant high-quality fertilizer of a kind of nutrient component, 2～more than 300,000,000 ton natural pond fertilizer of the annual generation of China at present, be mainly used in the seed soaking of various crops, do base manure, foliage fertilizer, part natural pond slag is used to breed fish, raises pigs, planting edible mushroom, and a part is abandoned becomes environomental pollution source.And with the natural pond slag as the relevant fungus body of medium culture, development can promote plant growth, the mycoprotein agricultural chemicals that improves the degeneration-resistant immunity of crop does not appear in the newspapers both at home and abroad as yet.
Summary of the invention
The present invention is intended to make full use of biogas fermentation residue natural pond slag, provides a kind of and can promote plant growth, improves the production method based on the induced anti-heterogenous fungus protein agricultural chemical for promoting crop growth of natural pond slag of the degeneration-resistant immunity of crop.
The production method of described induced anti-heterogenous fungus protein agricultural chemical for promoting crop growth based on the natural pond slag is characterized in that may further comprise the steps:
1. based on the production method of the induced anti-heterogenous fungus protein agricultural chemical for promoting crop growth of natural pond slag, it is characterized in that may further comprise the steps:
1) adopts solid culture or liquid culture CL and/or AA kind daughter bacteria;
2) culture medium preparation: the dry matter weight ratio in following each component is mixed, high-quality natural pond slag: barnyard grass grass=4: 1～1: 1, add the sucrose of gross weight 2.0%～4.0%, 5.0%～6.0% rice bran, 0.3%～0.8% urea is adjusted to 40%～45% with water content at last;
3) initial pH value with above-mentioned medium is adjusted to 6.0～7.0, under 121 ℃～125 ℃, the HTHP of 0.105～0.108MPa, sterilizes 20～30 minutes, and the cooling back is standby;
4) fermented and cultured: the CL of step 1) gained and/or AA kind daughter bacteria are seeded in the solid fermentation medium that step 3) obtains, 26 ℃～29 ℃ of temperature, enough throughputs are arranged, continuous darkness is cultivated or each cultivation that hockets in 12 hours of illumination/dark, stir once every day, carry out large scale fermentation and cultivated 7～10 days, obtain to contain the culture matrix of a large amount of CL and/or AA thalline, comprise spore and mycelia;
5) with above-mentioned steps 4) culture of gained, comprise culture matrix, fungus body, add that conventional adjuvant fully is mixed, drying, make induced anti-heterogenous fungus protein agricultural chemical for promoting crop growth with mechanical crushing.According to the employed Different Crop object of this albumen agricultural chemicals, adopt existing known conventional adjuvant, can be growth accelerator, as gibberellin etc.; Also can be short healthy and strong agent, as paclobutrazol, uniconazole P etc.
The production method of described induced anti-heterogenous fungus protein agricultural chemical for promoting crop growth based on the natural pond slag is characterized in that the method for described solid culture kind daughter bacteria may further comprise the steps:
1) preparation of CL and/or AA bacterial classification: the CL and/or the AA original strain expanding propagation on the PDA flat board that are kept on the PDA inclined-plane obtain the first order seed bacterium;
2) first order seed bacterium piece is inoculated into the barnyard grass grass on the medium of matrix, cultivates and obtain the secondary seed bacterium, comprise culture matrix and thalline.Its concrete cultural method is that " 02102975.X ", name are called existing open record in the patent of invention of " method of producing barnyard grass grass biocontrol microorganisms spore with barnyard grass grass matrix " in the patent No., does not repeat them here.
The production method of described induced anti-heterogenous fungus protein agricultural chemical for promoting crop growth based on the natural pond slag is characterized in that the method for described liquid culture kind daughter bacteria may further comprise the steps:
1) be basic culture solution with natural pond liquid, by natural pond liquid: the volume ratio preparation of water=4: 1～2: 1, with NaOH or HCl adjust pH to 6.8～7.2, add the sucrose or the glucose of gross weight 2.0%～4.0%, 0.3%～0.6% urea mixes the back and packs in the triangular flask by 250 ml container volumes, the 100 milliliters of zymotic fluid standards of packing into, sterilization is 20 minutes under the high temperature of 121 ℃～125 ℃ and 0.105～0.108MPa, condition of high voltage, and the cooling back is standby;
2) insert 3～4 in the above-mentioned triangular flask, diameter is 6～9mm, and cell age is 5～7 days CL and/or an AA bacterium piece, and at 150～200rpm, oscillation and fermentation cultivation is 7～10 days on 26～28 ℃ of constant temperature shaking tables, stops fermentation behind a large amount of hypha bodies to be formed;
3) the dried zymotic fluid of filter obtains hypha body, pulverizes hypha body as the secondary seed bacterium.
The production method of described induced anti-heterogenous fungus protein agricultural chemical for promoting crop growth based on the natural pond slag is characterized in that step 2) in: high-quality natural pond slag: barnyard grass grass=3: 1, sucrose 3.0%, rice bran 5.5%, urea 5.5%;
In the step 3): sterilization time is 25 minutes; In the step 4): cultivation temperature is 28 ℃, and the large scale fermentation incubation time is 9 days.
The production method of described induced anti-heterogenous fungus protein agricultural chemical for promoting crop growth based on the natural pond slag is characterized in that the particle diameter after the mechanical crushing is 10～25 μ m in the step 5).
The production method of described induced anti-heterogenous fungus protein agricultural chemical for promoting crop growth based on the natural pond slag, it is characterized in that: the configuration proportion of natural pond liquid and water is 3: 1 in the step 1) in the method for liquid culture kind daughter bacteria, adds 3.0% sucrose or glucose, 0.45% urea; Step 2) to go into cell age be 7 days CL and/or AA bacterium piece to the intermediate cam bottle graft, and the liquid fermentation and culture condition is an oscillation and fermentation cultivation 8 days on 170rpm, 28 ℃ the constant temperature shaking table.
The production method of described induced anti-heterogenous fungus protein agricultural chemical for promoting crop growth based on the natural pond slag, with biogas fermentation residue natural pond slag is primary raw material, be equipped with and induce the fungi particular matter of growth fast, add small amounts of inorganic salt element, form cheap, fast, the high-performance solid fermentation medium, large-scale production Curvularia lunata (Curvularia lunata, CL) and Alternariaspp (Alternaria altrnata, AA) fungus body, adopt the mechanical crushing technology to pulverize fermentation mixed culture (culture matrix and fungus body, down together), development patent medicine fertilizer has both, and can promote plant growth, improve the broad spectrum fungus albumen agricultural chemicals of the degeneration-resistant immunity of crop.Handle various single, double cotyledon plants with the seed dressing of fungi activator protein pesticide powder, the modes such as root, pouring, spraying of soaking, as paddy rice (long-grained nonglutinous rice, japonica rice, hybrid rice), wheat, corn, tomato, capsicum, watermelon, strawberry, soya bean, broad bean, fresh kidney beans, a variety of Chinese cabbage etc., the result shows that fungi activator protein agricultural chemicals can promote single, double cotyledon plant growth, improves the yield and quality, induce and make the resistance of deposits yields to multiple diseases, reach protection effect, reduce chemical fertilizer, pesticide dosage.Result of the test shows, handle paddy rice (rice blast, banded sclerotial blight, bakanae disease, bacterial leaf-blight), a variety of Chinese cabbage (soft rot, leaf spot), strawberry (powdery mildew, downy mildew) etc. with CL, AA mycoprotein, induced the resistance of these crops main fungi, bacteriosis.
Ratio that relates in the present specification and percentage composition are weight ratio and weight percentage except that specifying.
Embodiment
Now the present invention is described in further detail according to specific embodiment and test example thereof.
Embodiment 1:
1) the kind daughter bacteria of solid culture CL and/or AA bacterial classification: the preparation of (1) CL, AA bacterial classification, the CL and/or the AA original strain expanding propagation on the PDA flat board that are kept on the PDA inclined-plane obtain the first order seed bacterium; (2) first order seed bacterium piece is inoculated into the barnyard grass grass on the medium of matrix, cultivates the secondary seed bacterium.
2) natural pond slag culture medium preparation and fermented and cultured may further comprise the steps: (1) culture medium preparation, mix in the dry matter weight ratio of following each component, high-quality natural pond slag: barnyard grass grass=4: 1, sucrose 2.0%, rice bran 5.0%, urea 0.3% is adjusted to 40% with water content at last; (2) initial pH value with above-mentioned medium is adjusted to 7.0, under 121 ℃, the HTHP of 0.107MPa, sterilizes 30 minutes, and the cooling back is standby; (3) with the secondary seed bacterium of above-mentioned solid culture, comprise culture matrix and thalline, being seeded in the natural pond slag is in the solid fermentation medium of main matrix, 29 ℃ of temperature, enough throughputs are arranged, and continuous darkness is cultivated or illumination/dark respectively hocketed in 12 hours, and stir once every day, carry out large scale fermentation and cultivated 10 days, obtain to contain the culture matrix of a large amount of CL and/or AA bacterium.
3) with above-mentioned steps 2) the good culture of natural pond slag fermented and cultured of gained, comprise culture matrix, fungus body, add that adjuvant fully is mixed, drying, make crop allos fungi activator protein agricultural chemicals with mechanical crushing.
Embodiment 2:
1) the kind daughter bacteria of liquid culture CL and/or AA bacterial classification: (1) is basic culture solution with natural pond liquid, in natural pond liquid: the ratio preparation of water=4: 1, with NaOH or HCl adjust pH, add 2.0% sucrose or glucose, 0.3% urea, by pack into the standard dress triangular flask of 100 milliliters of zymotic fluids of 250 ml volumes containers, sterilization is 20 minutes under the high temperature of 121 ℃ and 0.106MPa, condition of high voltage, and the cooling back is standby; (2) to insert 3 ferfas be 5 days CL or AA bacterium piece age to above-mentioned triangular flask, is 150rpm in the liquid fermentation and culture condition, and oscillation and fermentation cultivation is 7 days on 28 ℃ the constant temperature shaking table, stops fermentation behind a large amount of hypha bodies to be formed; (3) the dried zymotic fluid of filter obtains hypha body, pulverizes hypha body and is used for following step 2 as the secondary seed bacterium) natural pond slag fermented and cultured;
2) natural pond slag culture medium preparation and fermented and cultured may further comprise the steps: (1) culture medium preparation, mix in the dry matter weight ratio of following each component, high-quality natural pond slag: barnyard grass grass=1: 1, sucrose 4.0%, rice bran 6.0%, urea 0.8% is adjusted to 45% with water content at last; (2) initial pH value with above-mentioned medium is adjusted to 7.0, under 121 ℃, the HTHP of 0.106MPa, sterilizes 30 minutes, and the cooling back is standby; (3) hypha body of aforesaid liquid fermented and cultured being pulverized body, to be seeded in the natural pond slag be in the solid fermentation medium of main matrix, 26 ℃ of temperature, enough throughputs are arranged, continuous darkness is cultivated or illumination/dark respectively hocketed in 12 hours, stir once every day, carry out large scale fermentation and cultivated 7 days, obtain to contain the culture matrix of a large amount of CL and/or AA bacterium.
3) with above-mentioned steps 2) the good culture of natural pond slag fermented and cultured of gained, comprise culture matrix, fungus body, add that adjuvant fully is mixed, drying, make crop allos fungi activator protein agricultural chemicals with mechanical crushing.
Embodiment 3:
1) the kind daughter bacteria of liquid culture CL and/or AA bacterial classification: (1) is basic culture solution with natural pond liquid, in natural pond liquid: the ratio preparation of water=3: 1, with NaOH or HCl adjust pH, add 3.0% sucrose or glucose, 0.45% urea, by pack into the standard dress triangular flask of 100 milliliters of zymotic fluids of 250 ml container volumes, sterilization is 25 minutes under the high temperature of 121 ℃ and 0.107MPa, condition of high voltage, and the cooling back is standby; (2) to insert 3 ferfas be 7 days CL or AA bacterium piece age to above-mentioned triangular flask, is 170rpm in the liquid fermentation and culture condition, and oscillation and fermentation cultivation is 8 days on 28 ℃ the constant temperature shaking table, stops fermentation behind a large amount of hypha bodies to be formed; (3) the dried zymotic fluid of filter obtains hypha body, pulverizes hypha body and is used for following step 2 as the secondary seed bacterium) natural pond slag fermented and cultured;
2) natural pond slag culture medium preparation and fermented and cultured may further comprise the steps: (1) culture medium preparation, mix in the dry matter weight ratio of following each component, high-quality natural pond slag: barnyard grass grass=3: 1, sucrose 3.0%, rice bran 5.5%, urea 5.5% is adjusted to 45% with water content at last; (2) initial pH value with above-mentioned medium is adjusted to 6.0, under 121 ℃, the HTHP of 0.106MPa, sterilizes 25 minutes, and the cooling back is standby; (3) hypha body of aforesaid liquid fermented and cultured being pulverized body, to be seeded in the natural pond slag be in the solid fermentation medium of main matrix, 28 ℃ of temperature, enough throughputs are arranged, continuous darkness is cultivated or illumination/dark respectively hocketed in 12 hours, stir once every day, carry out large scale fermentation and cultivated 9 days, obtain to contain the culture matrix of a large amount of CL and/or AA bacterium.
3) with above-mentioned steps 2) the good culture of natural pond slag fermented and cultured of gained, comprise culture matrix, fungus body, add that adjuvant fully is mixed, drying, make crop allos fungi activator protein agricultural chemicals with mechanical crushing.
Test 1: heterogenous fungus protein CL is to the influence of paddy growth
1.1 heterogenous fungus protein CL seed dressing or seed soaking Rice seeds treated are to the influence of paddy rice germination vigor and seedling quality
Heterogenous fungus protein seed dressing or seed soaking Rice seeds treated see Table 1 to the germination vigor of seed and the influence of seedling quality.Sprout the initial stage in germination, the heterogenous fungus protein Rice seeds treated is compared with the clear water contrast, improves germination rate, the elongation of induced bud and root.To paddy rice 3 leaves during the phase, handle rice shoot and increase 1～2cm; To 5 leaves during the phase, the hybrid rice that heterogenous fungus protein is handled, the rice shoot fresh weight of long-grained nonglutinous rice improve 16%～38% and 54%～70% than the clear water contrast respectively; During to 6 periods, the height of seedling comparison is according to increasing 4～6cm, and the rice shoot fresh weight that hybrid rice, long-grained nonglutinous rice are handled improves 34%～72% and 12%～31% than the clear water contrast respectively.Heterogenous fungus protein seed soaking or seed dressing Rice seeds treated are described, can promote the germination vigor of seed, seedling quality improves.
In addition, we plant in the test at dish and find, rice plant growth's speed that the CL mycoprotein is handled is more very fast than the clear water contrast, when the clear water contrast still is in 6 leaves during the phase, the rice shoot majority of handling illustrates that near 6 leaves, 1 heart seedling growth that heterogenous fungus protein was handled speeds up.
Table 1 heterogenous fungus protein CL seed dressing or seed soaking Rice seeds treated are to the influence of paddy rice germination vigor and seedling quality
*There were significant differences to be illustrated in 0.05 level
1.2 heterogenous fungus protein CL foliage-spray or water of the influence of root Rice seeds treated to paddy rice germination vigor and seedling quality
Carry out foliage-spray and water root with 5% mycoprotein during the phase at rice shoot 3 leaves, the results are shown in Table 2.To 6 leaves during the phase, no matter spray blade and still water root and handle the rice shoot base portion, hybrid rice is handled the rice shoot fresh weight increases by 45%～68%, and long-grained nonglutinous rice is handled rice shoot increases by 11%～18% than the clear water contrast, and fresh weight increases by 18%～40%; The rice plant seedling of handling during the phase to 7 leaves improves 9%～15% than the clear water contrast, and fresh weight increases by 15%～30%, and dry weight also increases by 15%～45%.
Table 2 heterogenous fungus protein CL foliage-spray or water root and handle the influence of rice seedling to rice seedling quality
*There were significant differences to be illustrated in 0.05 level.
With the data comparative analysis in table 1 and the table 2 as can be seen, during the phase, use diverse ways different at paddy rice 6 leaves to the seedling quality influence of paddy rice.Adopt seed soaking or seed dressing to handle, with foliage-spray or water root and handle and compare, more can promote the accumulation of dry matter, fresh weight and dry weight increase; Foliage-spray or water root and handle can increase plant height.
Test 2: heterogenous fungus protein CL is to the influence of vegetable growth
With heterogenous fungus protein preliminary treatment vegetable seeds, spray blade with heterogenous fungus protein then or water root processing vegetables seedling base portion, the results are shown in Table 3.The germination of vegetable seeds rate of soaking seed pretreated improves, and the germination rate of soya bean, tomato, capsicum and Chinese cabbage improves 20%, 8%, 17% and 8% separately.After the first time, seedling was handled 5 days, the seedling aspect ratio clear water contrast that soya bean, tomato, capsicum and Chinese cabbage are handled had improved 24%～32%, 11%～12%, 25%～26% and 12%～23% respectively.After handling 10 days for the second time, the seedling that soya bean, tomato, capsicum and Chinese cabbage are handled than the clear water contrast improved 23%～26%, 12%～19%, 23%～28% and 9%～30% respectively, root elongation increased by 30%～34%, 21%～33%, 16%～31% and 5%～13%, fresh weight increases by 21%～37%, 26%～34%, 28%～63% and 17%～21%, and dry weight increases by 5%～22.5%, 17%～23%, 20%～60% and 40%.
Table 3 heterogenous fungus protein CL is to the influence of vegetable growth
*There were significant differences to be illustrated in 0.05 level, and the numerical value unification all keeps 2 decimals in the table.
As can be seen from the results, no matter be foliage-spray, still watering root handles, can both promote the growth of vegetables seedling, after heterogenous fungus protein is handled, compare with the clear water contrast, the height of handling back vegetables seedling has on average increased 9%～32%, root elongation 5%～33%, fresh weight has increased weight 17%～63%.Can see simultaneously that watering root handles the growth that more helps vegetables than the sprinkling processing.
Simultaneously we also observe an interesting phenomenon, no matter spray to handle or water root and handle, and the color that can both deepen the vegetables blade has improved the photosynthesis of blade, helps the accumulation of matter energy.And can observe the tomato leaf color of handling obviously deepens than the clear water contrast.
Test 3: different biopesticide (medicine fertilizer) seed dressing Rice seeds treated, to the influence of paddy growth
From the result of the test of table 4 as can be seen, different heterogenous fungus proteins is handled and the power of planting rise the seed dressing Rice seeds treated, and the germination of seed is not had inhibitory action, can promote the elongation of root and bud; But seed is handled in the activator protein seed dressing, can suppress the germination rate of seed, reduces the vitality of seed, and is unfavorable to the paddy rice late growing stage.Rice shoot after heterogenous fungus protein seed dressing is handled is variant slightly with contrast during the phase at 5 leaves, 6 leaves during the phase effect comparatively obvious.To hybrid rice, 6 leaves make height of seedling increase 12%～13% than the clear water contrast during phase, and fresh weight improves 34%～74% respectively.To long-grained nonglutinous rice, 6 leaves make height of seedling increase 12%～14% than the clear water contrast during phase, and fresh weight improves 12%～31% respectively.Different short biomass processes japonica rice seeds to the not obviously influence of plant height, but has certain facilitation to increasing dry matter.After seed dressing is handled as heterogenous fungus protein, can increase fresh weight and increase about 50%.
The different short living beings seed dressing Rice seeds treated of table 4 is to the influence of paddy growth
Test 4: different biopesticide (medicine fertilizer) seed soaking Rice seeds treated, to the influence of paddy growth
As can be seen from Table 5, different biopesticide (medicine fertilizer) uses the seed soaking mode to handle seed, sprouts initial stage and rice shoot 3 leaves before the phase seed sprouting, does not all have significant difference.To paddy growth to 5 leaves during the phase, difference is obvious between the different processing.The seedling quality that heterogenous fungus protein is handled all is better than other processing.The rice shoot plant height that heterogenous fungus protein is handled increases.Single heterogenous fungus protein is handled hybrid rice and is promoted plant height to increase by 17%～22%, and fresh weight increases by 59%～72%, makes the long-grained nonglutinous rice plant height increase by 9.2%～12%, and fresh weight increases by 13%～18%.To 6 leaves during the phase effect also remarkable.And after planting power liter and activator protein seed soaking processing seed, to the not influence of growth of rice seedling.
The different short living beings seed soaking Rice seeds treated of table 5 is to the influence of paddy growth
Test 5: different biopesticide (medicine fertilizer) foliage-spray rice leaf is to the influence of paddy growth
With different short living beings at paddy rice 3 leaves during the phase, carry out foliage-spray, time performance is not clearly in 6 periods to hybrid rice, but during to 7 periods, compare with the clear water contrast, the rice shoot plant height that single heterogenous fungus protein is handled has increased by 8～9cm, and plant height has increased by 12%～13%, and fresh weight has improved 32%～41%; To long-grained nonglutinous rice, at 6 leaves during the phase, the rice shoot plant height that single heterogenous fungus protein is handled contrasts with clear water all with fresh weight that there were significant differences, has increased by 6.6%～11% and 35%～50% respectively, and to 7 leaves during the phase, also comparing photograph has increased by 12%～16% and 30%～44%.In addition, can see with activator protein and handle hybrid rice that the growth of rice shoot is had facilitation, the accumulation effect that particularly increases dry matter is obvious, compares with the clear water contrast, can increase dry weight and reach 51%.
The different short living beings foliage-spray rice leaf of table 6 is to the influence of paddy growth
Test 6: different biopesticide (medicine fertilizer) waters root and handles the influence of rice seedling to paddy growth
During the phase, different short living beings dilutions is poured into the paddy rice base portion at 3 leaves, to 6 leaf after date observation survey seedling qualities.Can see with planting the contrast of power liter, activator protein and clear water and comparing that the heterogenous fungus protein of various combination is handled all has certain facilitation to the increase of rice seedling plant height and the accumulation of dry matter.Compare with the clear water contrast, at 6 leaves during the phase, the rice shoot plant height that heterogenous fungus protein is handled increases by 4～5cm, improved 4.5%～11%, fresh weight has increased by 22%～31%, and to 7 leaves during the phase, heterogenous fungus protein is handled with other processing section and reached utmost point significant difference, plant height has increased by 7.3%～15%, and fresh weight has increased weight 7.3%～40%.Can see simultaneously that to 7 leaves during the phase plant the rice shoot fresh weight that power rises and activator protein is handled and descend, the rice shoot fresh weight of the heterogenous fungus protein processing of additional mixing has also descended, and illustrates that these three different processing are in the accumulation that is unfavorable for paddy rice later stage rice shoot dry matter.
The different short living beings of table 7 are watered root and are handled the influence of rice seedling to paddy growth
Conclusion (of pressure testing):
1. adopt different biopesticide (medicine fertilizer) to handle paddy rice and compare test, as can be seen, single heterogenous fungus protein reagent has certain facilitation to the growth of rice seedling, show that mainly several aspects: a. handles the seed (containing seed dressing or seed soaking) of paddy rice with heterogenous fungus protein, still not obvious in seedling growth initial stage effect, manifest gradually to the short fruit of coming into force of 5 leaf after dates.Illustrate and use the heterogenous fungus protein Rice seeds treated, may improve the soil environment of plant root late growing stage, promote absorption and the utilization of plant soil nutrient.B. handle 3 leaf phase rice seedlings (contain foliage-spray and water root) with heterogenous fungus protein, to 6 leaves during the phase effect effect be not very outstanding, along with the prolongation of breeding time, to 7 leaves during the phase, to the plant height of rice seedling increase and the accumulation effect of dry matter all very obvious.And from whole structure, the effect that heterogenous fungus protein CL handles is better than the AA treatment effect.
2. seed is handled in heterologous protein seed soaking that mixes or seed dressing has certain facilitation to the growth of rice seedling, but blade spraying and water the root DeGrain.
3. activator protein seed dressing Rice seeds treated is unfavorable for the sprouting of seed, even the germination of seed is had inhibitory action.Activator protein waters root and handles the accumulation that rice seedling has suppressed rice dry matter on the contrary.
4. plant the power liter and spray blade or water root processing paddy rice, all be unfavorable for the growth of rice seedling, in order to Rice seeds treated, certain effect is arranged in early days, the later stage diminuendo.
5. heterogenous fungus protein is handled dissimilar rice varieties (hybrid rice, long-grained nonglutinous rice and japonica rice), effect difference.For hybrid rice, for long-grained nonglutinous rice, it is longer to the time that the rice shoot expression effect comes out that heterogenous fungus protein is handled the back, and effect is not quite similar.Heterogenous fungus protein is handled hybrid rice and can be promoted the rice shoot plant height to increase by 11%～22%, and fresh weight improves 25%～74%; Heterogenous fungus protein is handled long-grained nonglutinous rice and can be promoted the rice shoot plant height to increase by 9%～14%, and fresh weight improves 7%～44%; Heterogenous fungus protein is handled the not influence of japonica rice to plant height, reaches 50% but can improve fresh weight.Illustrate that heterogenous fungus protein more can promote the accumulation of the dry matter of hybrid rice and japonica rice plant.
6. result of the test shows, uses diverse ways different to the seedling quality influence of paddy rice.Adopt seed soaking or seed dressing to handle, with foliage-spray or water root and handle and compare, more can promote the accumulation of dry matter, fresh weight of plant and dry weight increase; And foliage-spray or water root and handle can increase plant height.The heterogenous fungus protein seed soaking is compared with seed dressing, and the processing of can finding to soak seed is very fast to the short living inducing action effect of paddy rice, compares with the effect of watering the root processing spraying blade, and it is faster than spraying effect to water the root treatment effect.Illustrate that different processing modes is not exclusively the same to the growth-promoting functions of paddy rice.
7. in order to strengthen the short fruit of coming into force of heterogenous fungus protein, can soak seed or seed dressing processing seed,, water root again or spray blade and handle, further promote growing of rice seedling then to seeding stage at the seed initial stage of sprouting.
Claims (5)
1. based on the production method of the induced anti-heterogenous fungus protein agricultural chemical for promoting crop growth of natural pond slag, it is characterized in that may further comprise the steps:
1) adopts solid culture or liquid culture CL and/or AA kind daughter bacteria;
2) culture medium preparation: the dry matter weight ratio in following each component is mixed, high-quality natural pond slag: barnyard grass grass=4: 1～1: 1, add the sucrose of gross weight 2.0%～4.0%, 5.0%～6.0% rice bran, 0.3%～0.8% urea is adjusted to 40%～45% with water content at last;
3) initial pH value with above-mentioned medium is adjusted to 6.0～7.0, under 121 ℃～125 ℃, the HTHP of 0.105～0.108MPa, sterilizes 20～30 minutes, and the cooling back is standby;
4) fermented and cultured: the CL of step 1) gained and/or AA kind daughter bacteria are seeded in the solid fermentation medium that step 3) obtains, 26 ℃～29 ℃ of temperature, enough throughputs are arranged, continuous darkness is cultivated or each cultivation that hockets in 12 hours of illumination/dark, stir once every day, carry out large scale fermentation and cultivated 7～10 days, obtain to contain the culture matrix of a large amount of CL and/or AA thalline;
5) with above-mentioned steps 4) culture of gained, comprise culture matrix, fungus body, add that conventional adjuvant fully is mixed, drying, make induced anti-heterogenous fungus protein agricultural chemical for promoting crop growth with mechanical crushing.
2. the production method of the induced anti-heterogenous fungus protein agricultural chemical for promoting crop growth based on the natural pond slag as claimed in claim 1 is characterized in that the method for described solid culture kind daughter bacteria may further comprise the steps:
1) preparation of CL and/or AA bacterial classification: the CL and/or the AA original strain expanding propagation on the PDA flat board that are kept on the PDA inclined-plane obtain the first order seed bacterium;
2) first order seed bacterium piece is inoculated into the barnyard grass grass on the medium of matrix, cultivates and obtain the secondary seed bacterium.
3. the production method of the induced anti-heterogenous fungus protein agricultural chemical for promoting crop growth based on the natural pond slag as claimed in claim 1 is characterized in that the method for described liquid culture kind daughter bacteria may further comprise the steps:
1) be basic culture solution with natural pond liquid, by natural pond liquid: the volume ratio preparation of water=4: 1～2: 1, with NaOH or HCL adjust pH to 6.8～7.2, add the sucrose or the glucose of gross weight 2.0%～4.0%, 0.3%～0.6% urea, mix the back and pack in the triangular flask by 250 ml container volumes, the 100 milliliters of zymotic fluid standards of packing into, sterilization is 20 minutes under the high temperature of 121 ℃～125 ℃ and 0.105～0.108MPa, condition of high voltage, and cooling is afterwards standby;
2) insert 3～4 in the above-mentioned triangular flask, diameter is 6～9mm, and cell age is 5～7 days CL and/or an AA bacterium piece, and at 150～200rpm, oscillation and fermentation cultivation is 7～10 days on 26～28 ℃ of constant temperature shaking tables, stops fermentation behind a large amount of hypha bodies to be formed;
3) the dried zymotic fluid of filter obtains hypha body, pulverizes hypha body as the secondary seed bacterium.
4. the production method of the induced anti-heterogenous fungus protein agricultural chemical for promoting crop growth based on the natural pond slag as claimed in claim 1 is characterized in that the particle diameter after the mechanical crushing is 10～25 μ m in the step 5).
5. the production method of the induced anti-heterogenous fungus protein agricultural chemical for promoting crop growth based on the natural pond slag as claimed in claim 3, it is characterized in that: the configuration proportion of natural pond liquid and water is 3: 1 in the step 1) in the method for liquid culture kind daughter bacteria, adds 3.0% sucrose or glucose, 0.45% urea; Step 2) to go into cell age be 7 days CL and/or AA bacterium piece to the intermediate cam bottle graft, and the liquid fermentation and culture condition is an oscillation and fermentation cultivation 8 days on 170rpm, 28 ℃ the constant temperature shaking table.
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Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JPH0748214A (en) * | 1993-08-05 | 1995-02-21 | Mitsui Eng & Shipbuild Co Ltd | Agent for promoting resistance of plant to pathogenic microbe |
| US20040052776A1 (en) * | 2002-09-12 | 2004-03-18 | National Taiwan University | Method for inhibiting pathogenic fungi in plants using bacillus amyloliquefaciens |
| CN1219051C (en) * | 2002-02-10 | 2005-09-14 | 中国水稻研究所 | Method for producing Bicao anti-bateria spore using Bicao substrate |
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Patent Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JPH0748214A (en) * | 1993-08-05 | 1995-02-21 | Mitsui Eng & Shipbuild Co Ltd | Agent for promoting resistance of plant to pathogenic microbe |
| CN1219051C (en) * | 2002-02-10 | 2005-09-14 | 中国水稻研究所 | Method for producing Bicao anti-bateria spore using Bicao substrate |
| US20040052776A1 (en) * | 2002-09-12 | 2004-03-18 | National Taiwan University | Method for inhibiting pathogenic fungi in plants using bacillus amyloliquefaciens |
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