CN100403885C - Method for breeding Sanyou portunid - Google Patents
Method for breeding Sanyou portunid Download PDFInfo
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- CN100403885C CN100403885C CNB2007100667462A CN200710066746A CN100403885C CN 100403885 C CN100403885 C CN 100403885C CN B2007100667462 A CNB2007100667462 A CN B2007100667462A CN 200710066746 A CN200710066746 A CN 200710066746A CN 100403885 C CN100403885 C CN 100403885C
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- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02A—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE
- Y02A40/00—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production
- Y02A40/80—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production in fisheries management
- Y02A40/81—Aquaculture, e.g. of fish
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Abstract
A reproduction method for triwart protunid is characterized by that the water temp suitable for reproduction is 20-22 deg.C and its flea-shaped larvae are in shock state for 0.5-20 min by increasing or decreasing the water temp by 12-22 deg.C. The female rate can be increased to 65% or more.
Description
Technical field
The present invention relates to the propagation method of a kind of crab, relate in particular to a kind of propagation method of Portunus trituberculatus Miers.
Background technology
Portunus trituberculatus Miers was to fish for acquisition from the ocean in the past, now propagate artificially on a large scale, in order to satisfy a large amount of needs of culturing Portunus trituberculatus Miers, therefore people have carried out the artificial work of growing seedlings, people mating not oogenesis female crab or the female crab of oogenesis in artificial breeding pond, breed (laying eggs and hatching) as close crab and cultivate, its suitable breeding water temperature is 20 ℃～22 ℃, the embryo development procedure of Portunus trituberculatus Miers comprises fertilized egg, cleavage stage, blastula stage, gastrul stage, zoea stage in nauplius stage and the film in the film, zoea behind the membrane was grown through the fourth phase, metamorphosis is megalops larva, and the megalops larva remetaboly is young crab; By the animal reproduction rule, the male and female ratio of zoea under the normal condition behind the membrane and the young crab of cultivating respectively is about 50%.Portunus trituberculatus Miers is popular a kind of seafood, and its nutritive value and economic worth are all very high, especially the female crab price of Portunus trituberculatus Miers is higher than the price of male crab, can reach a times of male crab, so improve the ratio of female crab in the seed that raisers wish to be purchased, to reach better economic benefit.
Summary of the invention
Technical problem to be solved by this invention provides a kind of propagation method of Portunus trituberculatus Miers, and it has the ratio that improves the female young crab of Portunus trituberculatus Miers in reproductive process.
The present invention solves the problems of the technologies described above the technical scheme that is adopted: a kind of propagation method of Portunus trituberculatus Miers, its suitable breeding water temperature is 20 ℃～22 ℃, its embryonic development period, comprise nauplius stage and the interior zoea stage of film in fertilized egg, cleavage stage, blastula stage, gastrul stage, the film in order, characteristics are zoea stage processing of suffering a shock in described film, the temperature difference of the water temperature of described shock and described suitable breeding water temperature is ± 12 ℃～22 ℃, and the time of described shock is 0.5min～20min.
Described shock is a heat shock, and the water temperature of described heat shock is 38.5 ℃～44.0 ℃, and the time of described heat shock is 0.5min～2min.
The water temperature of described heat shock is 40.0 ℃～41.0 ℃, and the time of described heat shock is 1.5min.
Described shock is a cold shock, and the water temperature of described cold shock is 5.5 ℃～8.0 ℃, and the time of described cold shock is 10min～20min.
The water temperature of described cold shock is 6.5 ℃～7.5 ℃, and the time of described cold shock is 15min.
Compared with prior art, the invention has the advantages that because the processing of in film, suffering a shock in the zoea stage, the temperature difference of the water temperature of shock and its suitable breeding water temperature is ± 12 ℃～22 ℃, the time of shock is 0.5min～20min, after zoea stage is handled through shock in film like this, variation has taken place in the male and female ratio of zoea behind the membrane and the young crab of cultivating, the ratio that makes breeding cultivate the female young crab of back acquisition reaches more than 57%, especially pass through the processing of cold shock, the ratio that the female young crab in back is cultivated in breeding reaches more than 65%, so just improve the artificial propagation Portunus trituberculatus Miers and propagated the economic benefit of Portunus trituberculatus Miers artificially, also satisfied the requirement that people like female crab.
Experimental group 1: the mating that 5 seas are caught the not female Portunus trituberculatus Miers of oogenesis is entrusted one's child to the care of sb. in the artificial propagation pond as close crab, after progressively improving water temperature to 20 ℃～22 ℃, stablize water temperature, allow it lay eggs and hatching, often carry out microscopy, when embryonic development in film during zoea stage, close crab taken out to put in the seawater shock pond that water temperature is controlled at 40.0 ℃～41.0 ℃ carry out heat shock, the time of heat shock is 1.5min, and then close crab put back in the artificial propagation pond continues to cultivate, become young crab to the zoea cultivation of hatching after, random sampling, the male and female of the young crab of microscopy obtain the experimental result in the following table:
Experimental group 2: identical with experiment 1 experimental technique, the water temperature of experimental group heat shock that different is is 43. ℃～44.0 ℃, and the time of heat shock is 0.7min, obtains the experimental result in the following table:
Experimental group 3: identical with experiment 1 experimental technique, the water temperature of experimental group heat shock that different is is 38.5 ℃～39.5 ℃, and the time of heat shock is 2min, obtains the result in the following table:
Experimental group 4: identical with the experimental technique of experiment 1, different is to handle with cold shock to experimental group, and the water temperature of cold shock is 6.5 ℃～7.5 ℃, and the processing time is 15min, obtains following experimental result:
Experimental group 5: identical with experiment 4 experimental technique, the water temperature of different is experimental group cold shock is 5.5 ℃～6.5 ℃, and the processing time is 20min, obtains the experimental result in the following table:
Control group: 5 seas catch mating not the female crab of oogenesis entrust one's child to the care of sb. in the artificial propagation pond as close crab, after progressively improving water temperature to 20 ℃～22 ℃, stablize water temperature, allow it lay eggs, pass through each period of embryonic development like this, cultivate the zoea membrane, after cultivation becomes young crab again, random sampling, the male and female of the young crab of microscopy obtain the experimental result in the following table:
From following table, can draw embryonic development at Portunus trituberculatus Miers and in film, give heat shock during zoea stage, the water temperature of heat shock is at 38.5 ℃～44.0 ℃, the time of heat shock is 0.5min～2min, and the ratio of the female young crab that obtains after the cultivation just surpasses normal value, reaches more than 57%; Give cold shock in the same film during zoea stage, the water temperature of cold shock is at 5.5 ℃～7.5 ℃, the time of cold shock is 15min～20min, the ratio of the female young crab that obtains after the cultivation just surpasses normal value, reach more than 65%, the processing and control group is suffered a shock, the ratio of the female young crab that obtains after the cultivation is 50.40%.
According to this experiment situation analysis, the present invention also can be applicable to other various crabs in the processing of suffering a shock of its embryonic development zoea stage in the film, also may improve the ratio of female young crab after its breeding, the present invention also may be applied in the breeding of crustacean, makes crustacean improve the ratio of female young crab after its breeding with the method.
Table: be the shared ratio of female young crab of breeding under the difference shock condition
| Title | Shock (water temperature/time) | The total number of elements of young crab of sampling | Female number of elements | Female sex rate |
| Experimental group 1 | 40.0℃～41.0℃/1.5min | 657 | 385 | 58.60％ |
| Experimental group 2 | 43.0℃～44.0℃/0.7min | 624 | 357 | 57.21％ |
| Experimental group 3 | 38.5℃～39.5℃/2min | 621 | 359 | 57.80％ |
| Experimental group 4 | 6.5℃～7.5℃/10min | 674 | 453 | 67.21％ |
| Experimental group 5 | 5.5℃～6.5℃/20min | 656 | 432 | 65.85％ |
| Control group | Do not have | 631 | 318 | 50.40％ |
Embodiment
Below in conjunction with embodiment the present invention is described in further detail.
Embodiment 1
5 seas catch mating not the female crab of the Portunus trituberculatus Miers of oogenesis as close crab, the oxygenation transportation, entrust one's child to the care of sb. after the sterilization in the artificial propagation pond, after progressively improving water temperature to 20 ℃～22 ℃, stablize water temperature, allow it lay eggs and hatching, often carry out microscopy, when embryonic development in film during zoea stage, close crab taken out to put in the seawater shock pond that water temperature is controlled at 40.0 ℃～41.0 ℃ carry out heat shock, the time of heat shock is 1.5min, and then close crab is put back to continue cultivation in the artificial propagation pond, to the zoea membrane, and then cultivation becomes young crab.
Embodiment 2
Embodiment is with above-mentioned embodiment 1, the different just water temperatures of heat shock be controlled at 43.0 ℃～44.0 ℃, the time of heat shock is 0.7min.
Embodiment 3
Embodiment is with above-mentioned embodiment 1, the different just water temperatures of heat shock be controlled at 38.5 ℃～39.5 ℃, the time of heat shock is 2.0min.
Embodiment 4
Embodiment is with above-mentioned embodiment 1, the different cold shocks that just carry out, the water temperature of cold shock is 6.5 ℃～7.5 ℃, the time of cold shock is 15min.
Embodiment 5
Embodiment is with above-mentioned embodiment 4, the different just water temperatures of cold shock be 5.5 ℃～6.5 ℃, the time of cold shock is 20min.
The female crab of the Portunus trituberculatus Miers of also available oogenesis is as close crab in the foregoing description.
Claims (5)
1. the propagation method of a Portunus trituberculatus Miers, its suitable breeding water temperature is 20 ℃～22 ℃, its embryonic development period, comprise nauplius stage and the interior zoea stage of film in fertilized egg, cleavage stage, blastula stage, gastrul stage, the film in order, it is characterized in that in described film the zoea stage processing of suffering a shock, the temperature difference of the water temperature of described shock and described suitable breeding water temperature is ± 12 ℃～22 ℃, and the time of described shock is 0.5min～20min.
2. the propagation method of a kind of Portunus trituberculatus Miers as claimed in claim 1 is characterized in that described shock is a heat shock, and the water temperature of described heat shock is 38.5 ℃～44.0 ℃, and the time of described heat shock is 0.5min～2min.
3. the propagation method of a kind of Portunus trituberculatus Miers as claimed in claim 2, the water temperature that it is characterized in that described heat shock is 40.0 ℃～41.0 ℃, the time of described heat shock is 1.5min.
4. the propagation method of a kind of Portunus trituberculatus Miers as claimed in claim 1 is characterized in that described shock is a cold shock, and the water temperature of described cold shock is 5.5 ℃～8.0 ℃, and the time of described cold shock is 10min～20min.
5. the propagation method of a kind of Portunus trituberculatus Miers as claimed in claim 4, the water temperature that it is characterized in that described cold shock is 6.5 ℃～7.5 ℃, the time of described cold shock is 15min.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CNB2007100667462A CN100403885C (en) | 2007-01-18 | 2007-01-18 | Method for breeding Sanyou portunid |
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| Application Number | Priority Date | Filing Date | Title |
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| CNB2007100667462A CN100403885C (en) | 2007-01-18 | 2007-01-18 | Method for breeding Sanyou portunid |
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| CN101002548A CN101002548A (en) | 2007-07-25 |
| CN100403885C true CN100403885C (en) | 2008-07-23 |
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| CNB2007100667462A Expired - Fee Related CN100403885C (en) | 2007-01-18 | 2007-01-18 | Method for breeding Sanyou portunid |
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Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101703024B (en) * | 2009-07-03 | 2011-09-07 | 浙江海洋学院 | Method and device for cage propagation of Portunus trituberculatus Miers in coastal mudflat pond |
Families Citing this family (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102119675A (en) * | 2010-12-30 | 2011-07-13 | 广东海洋大学 | Method for cultivating portunus pelagicus |
| CN103651199B (en) * | 2013-12-04 | 2016-03-16 | 北海市振发海洋产业科技有限公司 | The cultural method of a kind of Portunus pelagicus young |
| CN103875566A (en) * | 2014-03-21 | 2014-06-25 | 苏州市阳澄湖现代农业产业园特种水产养殖有限公司 | Method for ecologically breeding portunus trituberculatus in ponds |
Citations (7)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US4697546A (en) * | 1986-05-30 | 1987-10-06 | Purdue Research Foundation | Method for production of tetraploid channel catfish |
| RU2045896C1 (en) * | 1992-12-08 | 1995-10-20 | Всероссийский научно-исследовательский институт прудового рыбного хозяйства | Fish genotype restoration method |
| EP0934697A1 (en) * | 1997-12-09 | 1999-08-11 | Navital S.a.r.l. | A method for the cultivation, conditioning and storage of shell fish independently of the season and an installation for operating of this method |
| WO1999056534A1 (en) * | 1998-04-30 | 1999-11-11 | Ribes Andre | Method for reproducing and rearing silurus glanis in particular in aquatic medium and implementing device |
| US20030229211A1 (en) * | 1988-06-23 | 2003-12-11 | Wainwright Norman R. | Endotoxin binding and neutralizing protein and uses thereof |
| CN1141018C (en) * | 2001-05-16 | 2004-03-10 | 中国科学院海洋研究所 | Inductest for triploid of Eriocheir sinensia |
| CN1763180A (en) * | 2004-10-22 | 2006-04-26 | 中国科学院海洋研究所 | The triploid preparation method of different sorts prawn |
-
2007
- 2007-01-18 CN CNB2007100667462A patent/CN100403885C/en not_active Expired - Fee Related
Patent Citations (7)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US4697546A (en) * | 1986-05-30 | 1987-10-06 | Purdue Research Foundation | Method for production of tetraploid channel catfish |
| US20030229211A1 (en) * | 1988-06-23 | 2003-12-11 | Wainwright Norman R. | Endotoxin binding and neutralizing protein and uses thereof |
| RU2045896C1 (en) * | 1992-12-08 | 1995-10-20 | Всероссийский научно-исследовательский институт прудового рыбного хозяйства | Fish genotype restoration method |
| EP0934697A1 (en) * | 1997-12-09 | 1999-08-11 | Navital S.a.r.l. | A method for the cultivation, conditioning and storage of shell fish independently of the season and an installation for operating of this method |
| WO1999056534A1 (en) * | 1998-04-30 | 1999-11-11 | Ribes Andre | Method for reproducing and rearing silurus glanis in particular in aquatic medium and implementing device |
| CN1141018C (en) * | 2001-05-16 | 2004-03-10 | 中国科学院海洋研究所 | Inductest for triploid of Eriocheir sinensia |
| CN1763180A (en) * | 2004-10-22 | 2006-04-26 | 中国科学院海洋研究所 | The triploid preparation method of different sorts prawn |
Non-Patent Citations (4)
| Title |
|---|
| 中华绒螯蟹多倍体的诱导研究：Ⅱ.热休克诱导中华绒螯蟹三倍体胚胎和四倍体蚤状幼体. 陈立侨，赵云龙，周忠良，唐灵燕，陈魏，堵南山，赖伟.动物学报，第43卷第4期. 1997 |
| 中华绒螯蟹多倍体的诱导研究：Ⅱ.热休克诱导中华绒螯蟹三倍体胚胎和四倍体蚤状幼体. 陈立侨，赵云龙，周忠良，唐灵燕，陈魏，堵南山，赖伟.动物学报，第43卷第4期. 1997 * |
| 热休克诱导斑节对虾四倍体的初步研究. 王红勇，吴洪流，黄勃.海南大学学报自然科学版，第22卷第2期. 2004 |
| 热休克诱导斑节对虾四倍体的初步研究. 王红勇，吴洪流，黄勃.海南大学学报自然科学版，第22卷第2期. 2004 * |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101703024B (en) * | 2009-07-03 | 2011-09-07 | 浙江海洋学院 | Method and device for cage propagation of Portunus trituberculatus Miers in coastal mudflat pond |
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