CN100381373C - Method for cultivating nitrified granular sludge using anaerobic granular sludge - Google Patents
Method for cultivating nitrified granular sludge using anaerobic granular sludge Download PDFInfo
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- CN100381373C CN100381373C CNB200610038726XA CN200610038726A CN100381373C CN 100381373 C CN100381373 C CN 100381373C CN B200610038726X A CNB200610038726X A CN B200610038726XA CN 200610038726 A CN200610038726 A CN 200610038726A CN 100381373 C CN100381373 C CN 100381373C
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- waste water
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- 239000010802 sludge Substances 0.000 title claims abstract description 45
- 238000000034 method Methods 0.000 title claims abstract description 20
- 239000002351 wastewater Substances 0.000 claims abstract description 33
- XKMRRTOUMJRJIA-UHFFFAOYSA-N ammonia nh3 Chemical compound N.N XKMRRTOUMJRJIA-UHFFFAOYSA-N 0.000 claims abstract description 24
- 239000011573 trace mineral Substances 0.000 claims abstract description 17
- 235000013619 trace mineral Nutrition 0.000 claims abstract description 17
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims abstract description 9
- 239000002054 inoculum Substances 0.000 claims abstract description 8
- QVGXLLKOCUKJST-UHFFFAOYSA-N atomic oxygen Chemical compound [O] QVGXLLKOCUKJST-UHFFFAOYSA-N 0.000 claims abstract description 7
- 229910052760 oxygen Inorganic materials 0.000 claims abstract description 7
- 239000001301 oxygen Substances 0.000 claims abstract description 7
- OKTJSMMVPCPJKN-UHFFFAOYSA-N Carbon Chemical compound [C] OKTJSMMVPCPJKN-UHFFFAOYSA-N 0.000 claims abstract description 5
- 229910052799 carbon Inorganic materials 0.000 claims abstract description 5
- 230000001546 nitrifying effect Effects 0.000 claims description 21
- QGZKDVFQNNGYKY-UHFFFAOYSA-O Ammonium Chemical compound [NH4+] QGZKDVFQNNGYKY-UHFFFAOYSA-O 0.000 claims description 10
- 150000003016 phosphoric acids Chemical class 0.000 claims description 5
- UIIMBOGNXHQVGW-UHFFFAOYSA-M Sodium bicarbonate Chemical compound [Na+].OC([O-])=O UIIMBOGNXHQVGW-UHFFFAOYSA-M 0.000 claims description 4
- 229910052759 nickel Inorganic materials 0.000 claims description 4
- 229910052796 boron Inorganic materials 0.000 claims description 3
- 229910052791 calcium Inorganic materials 0.000 claims description 3
- 229910052802 copper Inorganic materials 0.000 claims description 3
- 229910052742 iron Inorganic materials 0.000 claims description 3
- 229910052749 magnesium Inorganic materials 0.000 claims description 3
- 229910052748 manganese Inorganic materials 0.000 claims description 3
- 239000011734 sodium Substances 0.000 claims description 3
- 229910052725 zinc Inorganic materials 0.000 claims description 3
- 238000002360 preparation method Methods 0.000 claims description 2
- 229910000030 sodium bicarbonate Inorganic materials 0.000 claims description 2
- 235000017557 sodium bicarbonate Nutrition 0.000 claims description 2
- 238000010790 dilution Methods 0.000 claims 1
- 239000012895 dilution Substances 0.000 claims 1
- 229910019142 PO4 Inorganic materials 0.000 abstract 1
- OAICVXFJPJFONN-UHFFFAOYSA-N Phosphorus Chemical compound [P] OAICVXFJPJFONN-UHFFFAOYSA-N 0.000 abstract 1
- 239000003795 chemical substances by application Substances 0.000 abstract 1
- NBIIXXVUZAFLBC-UHFFFAOYSA-K phosphate Chemical compound [O-]P([O-])([O-])=O NBIIXXVUZAFLBC-UHFFFAOYSA-K 0.000 abstract 1
- 239000010452 phosphate Substances 0.000 abstract 1
- 229910052698 phosphorus Inorganic materials 0.000 abstract 1
- 239000011574 phosphorus Substances 0.000 abstract 1
- 241000108664 Nitrobacteria Species 0.000 description 11
- 230000003647 oxidation Effects 0.000 description 7
- 238000007254 oxidation reaction Methods 0.000 description 7
- IJGRMHOSHXDMSA-UHFFFAOYSA-N Atomic nitrogen Chemical compound N#N IJGRMHOSHXDMSA-UHFFFAOYSA-N 0.000 description 4
- 241000894006 Bacteria Species 0.000 description 3
- FRXSZNDVFUDTIR-UHFFFAOYSA-N 6-methoxy-1,2,3,4-tetrahydroquinoline Chemical compound N1CCCC2=CC(OC)=CC=C21 FRXSZNDVFUDTIR-UHFFFAOYSA-N 0.000 description 2
- 230000003321 amplification Effects 0.000 description 2
- 238000006243 chemical reaction Methods 0.000 description 2
- 230000000694 effects Effects 0.000 description 2
- 238000011081 inoculation Methods 0.000 description 2
- 238000006396 nitration reaction Methods 0.000 description 2
- 229910052757 nitrogen Inorganic materials 0.000 description 2
- 238000003199 nucleic acid amplification method Methods 0.000 description 2
- 238000012545 processing Methods 0.000 description 2
- 239000010865 sewage Substances 0.000 description 2
- 241001453382 Nitrosomonadales Species 0.000 description 1
- JVMRPSJZNHXORP-UHFFFAOYSA-N ON=O.ON=O.ON=O.N Chemical compound ON=O.ON=O.ON=O.N JVMRPSJZNHXORP-UHFFFAOYSA-N 0.000 description 1
- VMHLLURERBWHNL-UHFFFAOYSA-M Sodium acetate Chemical compound [Na+].CC([O-])=O VMHLLURERBWHNL-UHFFFAOYSA-M 0.000 description 1
- UIIMBOGNXHQVGW-DEQYMQKBSA-M Sodium bicarbonate-14C Chemical compound [Na+].O[14C]([O-])=O UIIMBOGNXHQVGW-DEQYMQKBSA-M 0.000 description 1
- BDKZHNJTLHOSDW-UHFFFAOYSA-N [Na].CC(O)=O Chemical compound [Na].CC(O)=O BDKZHNJTLHOSDW-UHFFFAOYSA-N 0.000 description 1
- 230000001651 autotrophic effect Effects 0.000 description 1
- 238000013461 design Methods 0.000 description 1
- 238000011161 development Methods 0.000 description 1
- 230000007613 environmental effect Effects 0.000 description 1
- 239000008187 granular material Substances 0.000 description 1
- 230000007774 longterm Effects 0.000 description 1
- 230000004060 metabolic process Effects 0.000 description 1
- 230000000813 microbial effect Effects 0.000 description 1
- 238000012856 packing Methods 0.000 description 1
- FGIUAXJPYTZDNR-UHFFFAOYSA-N potassium nitrate Chemical compound [K+].[O-][N+]([O-])=O FGIUAXJPYTZDNR-UHFFFAOYSA-N 0.000 description 1
- 238000001556 precipitation Methods 0.000 description 1
- 238000009790 rate-determining step (RDS) Methods 0.000 description 1
- 239000001632 sodium acetate Substances 0.000 description 1
- 235000017281 sodium acetate Nutrition 0.000 description 1
- 238000009280 upflow anaerobic sludge blanket technology Methods 0.000 description 1
- 238000004065 wastewater treatment Methods 0.000 description 1
Classifications
-
- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02W—CLIMATE CHANGE MITIGATION TECHNOLOGIES RELATED TO WASTEWATER TREATMENT OR WASTE MANAGEMENT
- Y02W10/00—Technologies for wastewater treatment
- Y02W10/10—Biological treatment of water, waste water, or sewage
Abstract
The present invention relates to a method for utilizing anaerobic granular sludge to cultivate nitrified granular sludge. Inocula are put into an up-flow type aerobe reactor provided with a three phase separator firstly, and the inoculum is anaerobic granular sludge in a large anaerobic bioreactor. A proper number of culture accelerating agents composed of phosphate and trace elements need to be added into waste water containing ammonia nitrogen and organic carbon sources, or prepared simulated waste water, and the concentration of the phosphorus element is from 0.02 to 0.1 g/L. The waste water is diluted to be used as intake water of the reactor, of which the concentration of ammonia nitrogen is from 30 to 50 mg/L, to acclimatizing the inocula, and the stay time of the waste water in the reactor in the process of hydraulic acclimatization is from 8 to 36 h. The temperature in the reactor is kept between 20 to 35 DEG C, the pH value is kept between 7.5 to 8.5, and the concentration of dissolved oxygen is kept between 1.5 to 5 mg/L. When the ammonia nitrogen removing rate reaches 85% and the running is stable, the concentration of ammonia nitrogen in the intake water is increased gradually until the concentration of ammonia nitrogen in the intake water reaches 250 to 350 mg/L, the stable running is kept for 60 to 120 days, and nitrified granular sludge can be obtained in the reactor.
Description
Technical field
The present invention relates to a kind of method of utilizing anaerobic grain sludge to cultivate nitrifying granular activated sludge, belong to the biological wastewater treatment technology field.
Technical background
Biological denitrificaion is used widely because of its economy, effective having obtained in the denitrogenation processing of waste water.The upflowing bio-reactor of being furnished with triphase separator also is widely used.Yet, because nitrobacteria is a kind of growth velocity autotrophic bacteria extremely slowly, and very responsive to environmental factor, therefore be difficult to be implemented in and keep a large amount of nitrobacterias in the reactor, thereby cause the nitrated rate limiting step that becomes in the wastewater biological denitrificaion processing.Granule sludge is that a large amount of bacteriums assemble the particulate state microbial aggregate that growth forms, profile with rule, closely knit structure, with good precipitation threshold, utilize higher sludge concentration in their energy realization response devices, thereby help to realize that less reactor takes up an area of design, and can bear the sewage load and the impact load of higher concentration.Therefore, in bio-reactor, turn out and have highly active nitrifying granular activated sludge, can improve the nitrobacteria content in the reactor, improve the volumetric loading and the operation stability of reactor.
CN02112100.1 discloses a kind of startup method of anaerobic ammonium oxidation apparatus.The startup method of anaerobic ammonium oxidation apparatus is to adopt the biological nitration device to cultivate nitrobacteria earlier under aerobic condition, obtain the nitrifying granular activated sludge that has nitrification activity and anaerobic ammoxidation activity concurrently, again nitrifying granular activated sludge is dropped into anaerobic ammonium oxidation apparatus as inoculum.The operational condition of biological nitration device is: 20～35 ℃ of temperature, pH7.0～8.5, hydraulic detention time 5～15 hours; The operational condition of anaerobic ammonium oxidation apparatus is: 20～35 ℃ of temperature, pH7.0～8.5, hydraulic detention time 8～18 hours, the ratio of ammonia nitrogen concentration and nitrite nitrogen concentration be 1: 1.1～1.5 or the ratio of ammonia nitrogen concentration and nitre nitrogen concentration be 1: 0.8～1.2.
Above scheme is utilized nitrobacteria and metabolism diversity, and amplification nitrobacteria thalline under anaerobic carries out the Anammox reaction under aerobic condition; Utilize nitrobacteria can form the performance of nitrifying granular activated sludge, cultivate anaerobic ammonium oxidation granular sludge by nitrifying granular activated sludge; Utilize the good settling property of anaerobic ammonium oxidation granular sludge, realize the efficient operation of anaerobic sewage biological denitrifying device.The advantage of such scheme is: (1) has solved the difficult problem in anaerobic ammonium oxidation apparatus inoculum source; (2) it is slow to have solved the Anammox bacteria growing, is difficult for the difficult problem of amplification; (3) solve anaerobic ammonia oxidizing bacteria and in reaction unit, held a difficult problem of staying.But above-mentioned bottleneck still exists.The nitrobacteria thalline need increase under aerobic condition.
Summary of the invention
The objective of the invention is to hang down and problems such as clearance is low in order to solve the reactor volume load that causes owing to nitrobacteria concentration is low in the existing nitrator.At the problems referred to above, the invention provides a kind of nitrifying granular activated sludge and its cultural method.
The anaerobic grain sludge that utilizes that the present invention proposes is cultivated the method for nitrifying granular activated sludge, it is characterized in that this method comprises the steps:
1) the upflowing aerobe reactor of at first a certain amount of inoculum being packed into and being furnished with triphase separator; Inoculum is the anaerobic grain sludge in the large-scale anaerobic biological reactor;
2) waste water or preparation contain the simulated wastewater of ammonia nitrogen and organic carbon source, also need add an amount of culture promoter of being made up of phosphoric acid salt, trace element and sodium bicarbonate in the simulated wastewater;
3) described waste water or simulated wastewater being diluted to ammonia nitrogen concentration is that 30-50mg/L is intake as reactor, the hydraulic detention time of waste water in reactor is 8～36h, keep temperature in the reactor between 20～35 ℃, the pH value between 7.5～8.5, dissolved oxygen 1.5～5mg/L;
4) reach 85% and when stable when ammonia nitrogen removal frank, progressively improve the ammonia nitrogen concentration in the water inlet, reach 250-350mg/L, steady running for some time (general 60-120 day), in reactor, obtain nitrifying granular activated sludge until influent ammonium concentration.
In the present invention, trace element is one or more among Fe, Ca, Mg, Cu, B, Ni, Zn, Co, Mn and the Al in the described culture promoter, and every kind of trace trace element is 0.00002～0.02g/L in the concentration of simulated wastewater.
In the present invention, phosphoric acid salt is K in the described culture promoter
2HPO
4, KH
2PO
4, Na
2HPO
4And NaH
2PO
4In one or more, phosphoric is 0.02～0.1g/L in the concentration of simulated wastewater.
Advantage of the present invention is: the method that has proposed a kind of simple and easy to do cultivation nitrifying granular activated sludge, turned out nitrifying granular activated sludge and can keep reactor operation steady in a long-term, for the development and application of high-efficiency biological denitrification technology provides platform with superperformance.
Embodiment
At first with the anaerobic grain sludge upflowing aerobe reactor of packing into of inoculation, adopt the simulated wastewater that contains ammonia nitrogen and organic carbon source to start reactor then, in simulated wastewater, add an amount of culture promoter of forming by phosphoric acid salt, trace element and sodium bicarbonate, phosphoric is 0.02～0.1g/L in the concentration of simulated wastewater, trace element Fe, Ca, Mg, Cu, B, Ni, Zn, Co, Mn and the concentration of Al in waste water are 0.00002～0.02g/L, contain organic carbon source in the waste water.Adopt the method that progressively improves ammonia nitrogen concentration to carry out the domestication of nitrification, the simulated wastewater that will be diluted to ammonia nitrogen concentration earlier and be 30～50mg/L pumps into the upflowing aerobe reactor as the reactor water inlet and from the bottom, and the hydraulic detention time of domestication process is 8～36h.Keep the interior temperature of reactor between 20～35 ℃, the pH value is between 7.5～8.5, and dissolved oxygen 1.5～5mg/L to promote increasing fast of whole nitrobacteria flora, reaches the enrichment to nitrobacteria.When ammonia nitrogen removal frank reaches 85% and progressively increase ammonia nitrogen concentration in the water inlet when stable, reach 300mg/L until influent ammonium concentration.After steady running for some time, just can obtain nitrifying granular activated sludge in the reactor.
Embodiment 1
Anaerobic grain sludge 150ml in large industrialized UASB (upflow type anaerobic reactor) reactor is inoculated into top to be equipped with in the upflowing aerobe reactor of triphase separator, the useful volume of this reactor is 1.2L, and the sludge concentration in the inoculation post-reactor is about 5.5g SS/L.With being diluted to ammonia nitrogen concentration is that the simulated wastewater of 50mg/L is intake as reactor, has also added by K in the waste water
2HPO
4, KH
2PO
4, the trace element, NaHCO
3Culture promoter and the small amount of acetic acid sodium formed, the concentration of phosphoric is 0.025g/L in the simulated wastewater, trace element concentration is respectively 0.0052gFe/L, 0.0108gCa/L, 0.0024gMg/L, 0.00005gCu/L, 0.00002gB/L, 0.00004gNi/L, 0.00004gZn/L, 0.00002gCo/L, 0.00002gMn/L and 0.00002gAl/L, and the concentration of sodium acetate is 0.05gCOD/L.The operational condition of reactor is: hydraulic detention time is 12～18h, and temperature is between 25～32 ℃, and the pH value is between 7.5～8.5, and dissolved oxygen is controlled at 2.5～3.5mg/L.When ammonia nitrogen removal frank reaches 85% and when stable, progressively increase ammonia nitrogen concentration in the water inlet until reaching 300mg/L.Steady running obtained khaki nitrifying granular activated sludge in the reactor of the left and right sides in 70 days.This upflowing bio-reactor is at total nitrogen volumetric loading 2.0kgN/ (m
3D) under the condition, ammonia nitrogen removal frank can reach more than 85%, and has advantages of higher stability.
Itself there is trace element then need not add in the waste water.
Embodiment 2
Adopt the seed sludge identical with embodiment 1, culture promoter is by Na
2HPO
4, NaH
2PO
4, the trace element, NaHCO
3Form, the concentration of phosphoric is 0.05g/L in the waste water, and trace element concentration is respectively 0.0104gFe/L, 0.02gCa/L, 0.005gMg/L, 0.0001gCu/L, 0.00002gB/L, 0.0001gNi/L, 0.0001gZn/L and 0.00004gAl/L.The operational condition of reactor is: hydraulic detention time is 20～30h, temperature between 20～25 ℃, the pH value between 7.5～8.5, dissolved oxygen 1.5～2.5mg/L.All the other operating process are identical with embodiment 1, and cultured continuously obtained nitrifying granular activated sludge in the reactor of the left and right sides in 80 days.
Embodiment 3
Adopt the seed sludge identical with embodiment 1, culture promoter is by K
2HPO
4, KH
2PO
4, the trace element, NaHCO
3Form, the concentration of phosphoric is 0.08g/L in the waste water, and trace element concentration is respectively 0.02gFe/L, 0.02gCa/L, 0.01gMg/L, 0.0002gCu/L, 0.0001gB/L, 0.0002gNi/L, 0.0002gZn/L, 0.0001gCo/L, 0.0001gMn/L and 0.0001gAl/L.The operational condition of reactor is: hydraulic detention time is 10～15h, temperature between 25～32 ℃, the pH value between 7.5～8.5, dissolved oxygen 3.5～4.5mg/L.All the other operating process are identical with embodiment 1, and cultured continuously obtained nitrifying granular activated sludge in the reactor of the left and right sides in 75 days.
Claims (4)
1. utilize anaerobic grain sludge to cultivate the method for nitrifying granular activated sludge, it is characterized in that the upflowing aerobe reactor of earlier inoculum being packed into and being furnished with triphase separator; Inoculum is the anaerobic grain sludge in the large-scale anaerobic biological reactor; Waste water or preparation contain the simulated wastewater of ammonia nitrogen and organic carbon source, also need add the culture promoter of being made up of phosphoric acid salt, trace element and sodium bicarbonate in the simulated wastewater, and phosphoric is 0.02～0.1g/L in the concentration of simulated wastewater; Being 30-50mg/L as reactor with described wastewater dilution to ammonia nitrogen concentration, water inlet is tamed inoculum, the residence time is 8～36h in the waterpower domestication process of waste water in reactor, keep temperature in the reactor between 20～35 ℃, the pH value between 7.5～8.5, dissolved oxygen 1.5～5mg/L; When ammonia nitrogen removal frank reaches 85% and when stable, progressively improve the ammonia nitrogen concentration in the water inlet, reach 250-350mg/L until influent ammonium concentration, steady running 60-120 day, in reactor, obtain nitrifying granular activated sludge.
2. by the described method of utilizing anaerobic grain sludge to cultivate nitrifying granular activated sludge of claim 1, it is characterized in that described phosphoric acid salt is K
2HPO
4, KH
2PO
4, Na
2HPO
4And NaH
2PO
4In one or more.
3. by the described method of utilizing anaerobic grain sludge to cultivate nitrifying granular activated sludge of claim 1, it is characterized in that described trace element is one or more among Fe, Ca, Mg, Cu, B, Zn, Ni, Co, Mn or the Al, every kind of trace element is 0.00002～0.02g/L in the concentration of simulated wastewater.
4. by the described method of utilizing anaerobic grain sludge to cultivate nitrifying granular activated sludge of claim 3, it is characterized in that trace element is Ni or Co.
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|---|---|---|---|
| CNB200610038726XA CN100381373C (en) | 2006-03-09 | 2006-03-09 | Method for cultivating nitrified granular sludge using anaerobic granular sludge |
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|---|---|---|---|
| CNB200610038726XA CN100381373C (en) | 2006-03-09 | 2006-03-09 | Method for cultivating nitrified granular sludge using anaerobic granular sludge |
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|---|---|
| CN1821122A CN1821122A (en) | 2006-08-23 |
| CN100381373C true CN100381373C (en) | 2008-04-16 |
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Families Citing this family (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102092846B (en) * | 2010-12-15 | 2012-09-05 | 南京工业大学 | Continuous culture method for aerobic particle sludge with synchronous denitrification and carbon removal function |
| CN102219300B (en) * | 2011-05-16 | 2012-11-21 | 苏州科技学院 | Method for culturing anaerobic granular sludge in anaerobic baffled reactor |
| CN102167443B (en) * | 2011-05-23 | 2012-06-13 | 合肥工业大学 | Method for removing organic arsenic in cultivation wastewater by anaerobic granular sludge |
| CN102259975B (en) * | 2011-05-27 | 2013-02-27 | 甘肃金桥给水排水设计与工程(集团)有限公司 | Bio-augmentation technique for treating high ammonia nitrogen waste water under low temperature condition |
| CN102976485B (en) * | 2012-12-10 | 2013-12-04 | 东北电力大学 | Method and device for fast cultivation of anaerobic granular sludge for treating swine wastewater |
| CN112777736A (en) * | 2020-12-16 | 2021-05-11 | 中海油天津化工研究设计院有限公司 | Anaerobic nitrification method |
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| CN1401594A (en) * | 2002-06-13 | 2003-03-12 | 浙江大学 | Anaerobic sewage biological denitrifying device starting method |
| CN1579958A (en) * | 2004-05-17 | 2005-02-16 | 湖南大学 | Automatized sequential reaction unit for aerobic particle sludge culture and research |
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