CN100376887C - Test paper for detecting heavy metal cadmium rapidly, preparation method and application - Google Patents
Test paper for detecting heavy metal cadmium rapidly, preparation method and application Download PDFInfo
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- CN100376887C CN100376887C CNB2005100133790A CN200510013379A CN100376887C CN 100376887 C CN100376887 C CN 100376887C CN B2005100133790 A CNB2005100133790 A CN B2005100133790A CN 200510013379 A CN200510013379 A CN 200510013379A CN 100376887 C CN100376887 C CN 100376887C
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Abstract
The present invention relates to test paper, and a preparing method thereof and an application thereof for quickly detecting heavy metal cadmium. The test paper is prepared by that quantitative filter paper is used, the quantitative filter paper is soaked in 0.0015% to 0.0025% of victoria blue B chromogenic agent solution prepared from deionized water for 50 minutes to 70 minutes at normal temperature, and the soaked quantitative filter paper is naturally aired. The application of the test paper comprises the steps: ascorbic acid, potassium iodide and concentrated sulfuric acid are orderly added in solution to be detected after a water sample or a vegetable sample to be detected is counteracted so that the concentrations of the ascorbic acid, the potassium iodide and the concentrated sulfuric acid are respectively from 0.003 to 0.010 mol/L, 0.10 to 0.15 mol/L and 0.40 to 0.50 mol/L, and 1mL of phenanthroline alcohol solution with the concentration of 2% is added in the solution to be detected to be uniformly mixed to be detected; the test paper is put in a white PVC plastic tank of which the length is 9mm, the width is 7mm and the depth is 2mm, 30 mu L of the solution to be detected is taken to be dropped on the test paper, and the dropped test paper is put in the sunlight to react for 90 seconds to 180 seconds or is put in the non sunlight to react for 240 seconds to 360 seconds to be quantitatively compared with the standard color gradation. The test paper prepared by the present invention can quickly detecte environmental water samples and pollutant cadmium ions in agricultural products such as vegetables, etc. which are properly treated to achieve the purposes of protecting environment, promoting the safety production of nuisanceless agricultural products and ensuring human health.
Description
[technical field]:
The present invention relates to a kind of preparation of test paper and technical field of sample detection of detecting, particularly detect the preparation method of test paper of heavy metal cadmium and the application in detection thereof.
[background technology]:
Water is a kind of valuable natural resources, is the indispensable material of human lives, animal and plant growth and industrial and agricultural production.Current, China's water resource is being faced with the most serious since the dawn of human civilization pollution problem, not only causes enormous economic loss, the serious threat drinking water source, and greatly affect the sustainable development of people's healthy and national economy.The heavy metal of polluted-water has mercury, cadmium, lead, chromium, vanadium, cobalt, barium etc.Heavy metal so that various chemical states or chemical form exist will retain after entering the environment or the ecosystem, accumulates and move, and works the mischief.Heavy metal as discharging with waste water even concentration is little, also can accumulate in algae and bed mud, and by the body surface of fish and shellfish absorption, produce food chain and concentrate, thus the public hazards of causing.As the itai-itai of Japan, be due to the cadmium that is discharged by zinc metallurgy industry and cadmium electroplating industry.Cadmium is many-sided to the harm of tissue and organ, mainly is the harm to kidney, liver.In addition, cadmium also causes pulmonary edema, hypertension, anaemia, malacosteon and illnesss such as hyposphresia even forfeiture.
It is very general that China uses rural sewage disposal farm at present, and farmland and vegetable field soil and agricultural product are subjected to heavy metal pollution in various degree, produces cadmium rice as the sewage irrigated area, and the vegetables heavy metal cadmium is serious equally.Because it is heavy metal pollution has chronicity, disguise, therefore very big to human health damage; Simultaneously, a large amount of surface water and underground water are polluted by harmful heavy metal, cause having water to use, and drinking water source, town and country and economic sustainable development in serious threat.
At present, the analytical approach commonly used to heavy metal cadmium in water body, the vegetables mainly contains: (1) atomic absorption luminosity method; (2) atomic fluorescence method; (3) colourimetry; (4) anodic stripping voltammetry; (5) oscilloscopic polarography; (6) EDTA titrimetry etc., but these methods all adopt complex instrument to finish, and are not suitable for field quick detection.
[summary of the invention]:
The present invention seeks to solve existing analytical approach commonly used and be not suitable for the problem that field quick detection or use are subjected to certain limitation, the preparation method and the application of the wider fast detecting heavy metal cadmium test paper of a kind of scope of application are provided heavy metal cadmium in water body, the vegetables.
The test paper of fast detecting heavy metal cadmium provided by the invention is to adopt quantitative filter paper, and 0.0015%～0.0025% victoria blue B chromogenic reagent solution soak at room temperature via the deionized water preparation makes after drying naturally.
Concrete preparation method comprises successively:
---chromogenic reagent solution preparation: with deionized water victoria blue B being mixed with concentration is 0.0015%～0.0025% chromogenic reagent solution;
---filter paper soaks: the middling speed quantitative filter paper is put into the chromogenic reagent solution of step preparation, and normal temperature, and make filter paper can both fully be soaked 50～70 minutes time up and down;
---drying: the filter paper that will go up after soaking in the step takes out, and dries naturally;
---finished product and preservation: the filter paper after upward drying in the step is the test paper finished product of fast detecting heavy metal cadmium, and the test paper finished product is sheared the test-paper of growth 8mm, wide 6mm, places in the Brown Glass Brown glass bottles and jars only, and shady and cool drying place preserves.
The concrete applying step of this test paper in detection comprises:
---testing sample solution is prepared: after water sample to be measured or vegetable sample are cleared up processing, in solution to be measured, add ascorbic acid, potassium iodide, the concentrated sulphuric acid successively, make its concentration be respectively 0.001～0.010mol/L, 0.10～0.20mol/L and 0.20～0.30mol/L, to be measured behind the mixing;
---detect: the test paper of preparation is placed in the white PVC plastic channel of long 9mm, wide 7mm and dark 2mm, drawing 30 μ L drips of solution to be measured with micro-liquid getting device is added on the test paper, react that (being defined as of reaction time: placing response is 90～180 seconds under the daylight after 90～360 seconds, or placing response 240～360 seconds under the non-daylight), more quantitative with the standard color range.
Advantage of the present invention and good effect: the present invention has prepared a kind of cadmium ion quick, sensitive, that use is simple, selectivity is good and has detected test paper; the cadmium ion that pollutes in fast detecting environmental water sample and the agricultural product such as vegetables at the scene through suitably handling; reach the protection environment; promote the safety in production of non-polluted farm product, ensure the purpose of health.Ascorbic acid during detection is used does not participate in the chromogenic reaction of Cd (II) and developer, but in color development system, has vital role, a certain amount of ascorbic acid both can have been protected I ~ not oxidized, can shelter the interference of other heavy metal ion again with certain density adjacent luxuriant and rich with fragrance sound of vomiting quinoline solution; Cd (II) is highly stable with the complex anion of excessive I ~ formation, and easy and victoria blue B forms blue ternary complexes, has improved the sensitivity and the selectivity of test paper reaction; After the reaction of sulfuric acid and test paper, blank displaing yellow contains cadmium and shows blue in the liquid to be measured, and shade and cadmium concentration are proportionate.
[embodiment]:
Example example 1
The preparation of test paper comprises:
---chromogenic reagent solution preparation: with deionized water victoria blue B being mixed with concentration is 0.0020% chromogenic reagent solution;
---filter paper soaks: the middling speed quantitative filter paper is put into the chromogenic reagent solution of step preparation, and soak at room temperature, and the filter paper that constantly overturns keep filter paper can both fully be soaked 60 minutes time up and down;
---drying: the filter paper that will go up after soaking in the step takes out, and dries naturally;
---finished product and preservation: the filter paper after upward drying in the step is the test paper finished product of fast detecting heavy metal cadmium, and the test paper finished product is sheared the test-paper of growth 8mm, wide 6mm, places in the Brown Glass Brown glass bottles and jars only, and shady and cool drying place preserves.9～12 months holding times.
Example example 2
According to last routine preparation method, the victoria blue B solution concentration gets 0.0018%, and soak time was got 55 minutes, and all the other conditions are constant, make the test paper of fast detecting heavy metal cadmium.
Example example 3
Measure 25mL water sample to be measured, in the 150mL conical flask, add 15～20mL red fuming nitric acid (RFNA) and perchloric acid (3+1) acid mixture, heating is 240 minutes on electric hot plate, and it is in slight boiling condition its chien shih, places cooling thereafter.Add ascorbic acid, potassium iodide and the concentrated sulphuric acid in the solution to be measured to above-mentioned clearing up successively, make its concentration be respectively 0.005mol/L, 0.15mol/L and 0.25mol/L, adding 1mL concentration is 2% adjacent luxuriant and rich with fragrance sound of vomiting quinoline alcoholic solution, and is to be measured behind the mixing.The test-paper of preparation is placed in the white PVC plastic channel of long 9mm, wide 7mm and dark 2mm, drawing 30 μ L mixed solution to be measured with micro-liquid getting device is added drop-wise on the test-paper, placing response is 120 seconds under the daylight, or placing response after 300 seconds under the non-daylight, and is more quantitative with the standard color range.
Example example 4
Take by weighing about 25.0g pakchoi in the 150mL conical flask, add 15～20mL red fuming nitric acid (RFNA) and perchloric acid (3+1) acid mixture, heating is 300 minutes on electric hot plate, and it is in slight boiling condition its chien shih, places cooling thereafter.Add ascorbic acid, potassium iodide and the concentrated sulphuric acid in the solution to be measured to above-mentioned clearing up successively, make its concentration be respectively 0.006mol/L, 0.18mol/L and 0.26mol/L, adding 1mL concentration is 2% adjacent luxuriant and rich with fragrance sound of vomiting quinoline alcoholic solution, and is to be measured behind the mixing.The test-paper of preparation is placed in the white PVC plastic channel of long 9mm, wide 7mm and dark 1mm, accurately drawing 30 μ L mixed solution to be measured with micro-liquid getting device is added drop-wise on the test-paper, placing response is 150 seconds under the daylight, or placing response after 330 seconds under the non-daylight, and is more quantitative with the standard color range.
Claims (4)
1. the test paper of a fast detecting heavy metal cadmium, it is characterized in that this test paper adopt quantitative filter paper, via the 0.0015%-0.0025% victoria blue B chromogenic reagent solution soak at room temperature of deionized water preparation, make after drying naturally.
2. the preparation method of the described test paper of claim 1 is characterized in that this method comprises successively:
---chromogenic reagent solution preparation: victoria blue B is mixed with the chromogenic reagent solution that concentration is 0.0015%-0.0025% with deionized water;
---filter paper soaks: quantitative filter paper is put into the chromogenic reagent solution of step preparation, and normal temperature, and make filter paper can both fully be soaked time 50-70 minute up and down;
---drying: the filter paper that will go up after soaking in the step takes out, and dries naturally;
---finished product: the filter paper after upward drying in the step is the test paper finished product of fast detecting heavy metal cadmium.
3. according to the preparation method of the described test paper of claim 2, it is characterized in that quantitative filter paper is the middling speed quantitative filter paper, the test paper finished product is sheared the test-paper of growth 8mm, wide 6mm, places in the Brown Glass Brown glass bottles and jars only, and shady and cool dry place preserves.
4. the detection of the test paper of the described fast detecting heavy metal cadmium of claim 1 is used, and it is characterized in that this detection applying step comprises:
---testing sample solution is prepared: after water sample to be measured or vegetable sample are cleared up processing, in solution to be measured, add ascorbic acid, potassium iodide, the concentrated sulphuric acid successively, make its concentration be respectively 0.001-0.010mol/L, 0.10-0.20mol/L, 0.20-0.30mol/L, to be measured behind the mixing;
---detect: the test paper of preparation is placed in the white PVC plastic channel of long 9mm, wide 7mm and dark 1mm, drawing 30 μ L drips of solution to be measured with micro-liquid getting device is added on the test paper, placing response 90-180 is after second under the daylight, or placing response 240-360 second under the non-daylight, with the contrast of standard color range, obtain testing result.
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Families Citing this family (8)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102507548B (en) * | 2011-10-12 | 2016-10-12 | 江南大学 | A kind of method and application of relevant reagent paper thereof preparing detection of heavy metal ion sensing test-paper based on inkjet technology |
| CN103776822A (en) * | 2013-09-12 | 2014-05-07 | 天津凯英科技发展有限公司 | Rapid heavy-metal detection method, as well as test paper and preparation method thereof |
| CN103760154A (en) * | 2013-09-12 | 2014-04-30 | 天津凯英科技发展有限公司 | Rapid heavy metal detection method, and detection test paper and preparation method thereof |
| CN104049028B (en) * | 2014-06-12 | 2017-02-15 | 白银有色集团股份有限公司 | Determination method for cadmium amount in copper slag |
| CN104316523A (en) * | 2014-10-31 | 2015-01-28 | 长安大学 | Method for rapidly detecting cadmium content in solid waste |
| CN107228855A (en) * | 2017-05-25 | 2017-10-03 | 贵州省烟草科学研究院 | The preparation method and its detection method of the test paper of heavy metal cadmium in a kind of quick detection tobacco leaf |
| CN113418930B (en) * | 2021-06-04 | 2024-05-10 | 西北工业大学 | Method for rapidly detecting whether surface coating of iron plate is complete |
| CN117538318B (en) * | 2024-01-10 | 2024-04-05 | 山东利尔康医疗科技股份有限公司 | Residual peracetic acid test card and preparation method thereof |
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| US6309797B1 (en) * | 2000-04-26 | 2001-10-30 | Spectra Group Limited, Inc. | Selectively colorable polymerizable compositions |
| CN1478731A (en) * | 2003-06-30 | 2004-03-03 | 雁北师范学院 | Preparation method of triazene polymer chelant and its application |
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Patent Citations (2)
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| US6309797B1 (en) * | 2000-04-26 | 2001-10-30 | Spectra Group Limited, Inc. | Selectively colorable polymerizable compositions |
| CN1478731A (en) * | 2003-06-30 | 2004-03-03 | 雁北师范学院 | Preparation method of triazene polymer chelant and its application |
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