CN100368408C - Method of extracting licorice flavone from licorice slag - Google Patents
Method of extracting licorice flavone from licorice slag Download PDFInfo
- Publication number
- CN100368408C CN100368408C CNB2005101195807A CN200510119580A CN100368408C CN 100368408 C CN100368408 C CN 100368408C CN B2005101195807 A CNB2005101195807 A CN B2005101195807A CN 200510119580 A CN200510119580 A CN 200510119580A CN 100368408 C CN100368408 C CN 100368408C
- Authority
- CN
- China
- Prior art keywords
- licorice
- extracting
- organic solvent
- glycyrrhiza residue
- licoflavone
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Expired - Fee Related
Links
Landscapes
- Medicines Containing Plant Substances (AREA)
Abstract
The present invention discloses a method of extracting licorice flavone from licorice slag, which comprises the following steps: licorice slag is extracted by organic solvent ethyl acetate by an arbitrary extraction method in cold soaking, warm soaking or back flow; extracting liquid is merged, and then, after extract for removing the organic solvent is dried, the licorice flavone is obtained. The present invention has the advantages of easy acquirement of raw materials, and the yield and the total flavone content of the organic solvent ethyl acetate are relatively high. Compared with the current process of extracting licorice flavone from licorice slag, the process has the advantages of simple process and low cost. The method of extracting licorice flavone from licorice slag is very ideal at present.
Description
Technical field:
The present invention relates to a kind of method of extracting licoflavone, especially relate to a kind of method of from glycyrrhiza residue, extracting licoflavone.
Background technology:
The Chinese medicine Radix Glycyrrhizae is the dry root or the root stock of glycyrrhizic legume (Glycyrrhiza uralensis Fisch.), glycyrrhiza inflate bat (Glycyrrhiza inflata Bat.) or glycyrrhiza glabra (Glycyrrhizaglabra L.).The flat flavor of its property is sweet, can and middle beneficial gas, moistening lung are eliminated the phlegm, clearing heat and detoxicating, tonifying spleen and stomach and coordinating the actions of various ingredients in a prescription.Be used for swelling and pain in the throat, cough, shortness of breath and palpitation, coughing with a lot of sputum, the empty pain of gastral cavity abdomen, cushion toxicity etc.Its main component is triterpenoid saponin (Potenlini etc.) and liquorice flavonoids compound.
At present many to the Study on extraction of Potenlini, also comparative maturity.But for the extraction process of flavones ingredient in the Radix Glycyrrhizae, then the investigator is less, and utilize to extract waste residue behind the Potenlini extract licoflavone just still less.Pharmacological experimental data shows that liquorice flavonoids compound has effects such as antiulcer agent, antispastic, antibiotic, antiviral, anti-oxidant, anticancer, anti-heart disorder.The classical extracting method of licoflavone has solvent extration, polymeric amide absorption method etc., but because reasons such as complex process, yield are low, cost height are not suitable for suitability for industrialized production.From Radix Glycyrrhizae, extract Radix Glycyrrhizae extractum and Potenlini now, produced a large amount of waste residues,, can produce huge benefit surely as being made full use of.
And the present method of from licorice slag, extracting licoflavone, it is the patent application of " 200310115114.2 " that application number is arranged, and the method relative complex of licoflavone is extracted in this patent application, and cost is higher.
Summary of the invention:
The object of the present invention is to provide a kind of method of from glycyrrhiza residue, extracting licoflavone.Extract licoflavone effectively in the useless glycyrrhiza residue that this method can produce from extract Radix Glycyrrhizae extractum and Potenlini production process, and its cost is low, technology is simple.
Purpose of the present invention is implemented by following technical scheme: the present invention includes following steps:
(1), with cold soaking, temperature soak or reflux in any one extracting method, with glycyrrhiza residue with organic solvent extraction 1～3 time; Described organic solvent is an ethyl acetate;
(2), united extraction liquid, reclaim organic solvent wherein;
(3), the extract that will remove organic solvent then is drying to obtain licoflavone.
Described cold soaking is at ambient temperature with 5～15 times solvent soaking glycyrrhiza residue, extracts with the method for diacolation then.
It is to be with 5～15 times solvent soaking glycyrrhiza residue and be incubated 1～5 hour under 30～55 ℃ in temperature that described temperature is soaked.
Described backflow is to make solvent refluxing and kept reflux state 1～3 hour with 5～15 times solvent soaking glycyrrhiza residue and heating.
Advantage of the present invention is:
1, raw material is easy to get: glycyrrhiza residue is a waste, and because the Radix Glycyrrhizae consumption is big, so there is a large amount of glycyrrhiza residues to need second stage employ; Used organic solvent ethyl acetate is modal organic solvent.But its yield is relative with general flavone content higher.
2, yield and general flavone content are all than higher.Yield can be stabilized in more than 4.00%; General flavone content then can be stabilized in more than 30%.
3, to extract the technology of licoflavone at present from glycyrrhiza residue simple for technology, and cost is low.It is the at present ideal method of from glycyrrhiza residue, extracting licoflavone.
Embodiment:
Embodiment 1 (warm lixiviate is got)
With glycyrrhiza residue 100 grams, be solvent extraction 3 times with the ethyl acetate, be to soak glycyrrhiza residue and be incubated 2 hours with 500 gram ethyl acetate under 50 ℃ in temperature; United extraction liquid reclaims ethyl acetate wherein; The extract that to remove organic solvent then is drying to obtain licoflavone 4.8 grams, and yield 4.80%, general flavone content are 30.8%.
Embodiment 2:
With glycyrrhiza residue 100 grams, be solvent extraction 2 times with the ethyl acetate, be to soak glycyrrhiza residue and be incubated 2 hours with 1000 gram ethyl acetate under 50 ℃ in temperature; United extraction liquid reclaims ethyl acetate wherein; The extract that to remove organic solvent then is drying to obtain licoflavone 4.78 grams, and yield is 4.78%, general flavone content 37.4%.
Embodiment 3 (cold soaking extraction)
With glycyrrhiza residue 100 grams, be solvent extraction 3 times with the ethyl acetate, be to soak glycyrrhiza residue with 1500 gram ethyl acetate under 25 ℃ in temperature, extract with the method for diacolation then; United extraction liquid reclaims ethyl acetate wherein; The extract that to remove organic solvent then is drying to obtain licoflavone 4.6 grams, and yield 4.60%, general flavone content are 33.6%.
Embodiment 4 (refluxing extraction)
With glycyrrhiza residue 100 grams, be solvent extraction 3 times with the ethyl acetate, soak glycyrrhiza residue with 1000 gram ethyl acetate, and heating makes solvent refluxing and kept reflux state 2 hours; United extraction liquid reclaims ethyl acetate wherein; The extract that to remove organic solvent then is drying to obtain licoflavone 5.6 grams, and yield 5.60%, general flavone content are 38.3%.
Claims (4)
1. a method of extracting licoflavone from glycyrrhiza residue is characterized in that, comprises following steps:
(1), with cold soaking, temperature soak or reflux in any one extracting method, with glycyrrhiza residue with organic solvent extraction 1～3 time; Described organic solvent is an ethyl acetate;
(2), united extraction liquid, reclaim organic solvent wherein;
(3), the extract that will remove organic solvent then is drying to obtain licoflavone.
2. the method for extracting licoflavone from glycyrrhiza residue according to claim 1 is characterized in that: described cold soaking is at ambient temperature with 5～15 times solvent soaking glycyrrhiza residue, extracts with the method for diacolation then.
3. the method for extracting licoflavone from glycyrrhiza residue according to claim 1 is characterized in that: it is to be with 5～15 times solvent soaking glycyrrhiza residue and be incubated 1～5 hour under 30～55 ℃ in temperature that described temperature is soaked.
4. the method for extracting licoflavone from glycyrrhiza residue according to claim 1 is characterized in that: described backflow is to make solvent refluxing and kept reflux state 1～3 hour with 5～15 times solvent soaking glycyrrhiza residue and heating.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CNB2005101195807A CN100368408C (en) | 2005-11-16 | 2005-11-16 | Method of extracting licorice flavone from licorice slag |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CNB2005101195807A CN100368408C (en) | 2005-11-16 | 2005-11-16 | Method of extracting licorice flavone from licorice slag |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN1752081A CN1752081A (en) | 2006-03-29 |
| CN100368408C true CN100368408C (en) | 2008-02-13 |
Family
ID=36679096
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CNB2005101195807A Expired - Fee Related CN100368408C (en) | 2005-11-16 | 2005-11-16 | Method of extracting licorice flavone from licorice slag |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN100368408C (en) |
Families Citing this family (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102552239B (en) * | 2011-12-31 | 2013-10-30 | 沈阳药科大学 | Method for preparing anti-inflammatory and anti-tumor active ingredient group from liquorice dregs and application thereof |
| CN104382773A (en) * | 2014-10-30 | 2015-03-04 | 洛阳蓝斯利科技有限公司 | Method for extracting licoflavone from liquorice dregs |
| CN114470033A (en) * | 2021-12-28 | 2022-05-13 | 新疆全安药业股份有限公司 | Preparation method and application of licorice flavone extract |
Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1207910A (en) * | 1997-08-08 | 1999-02-17 | 中国科学院新疆化学研究所 | Licorice total flavone extracting method |
| CN1359905A (en) * | 2001-12-15 | 2002-07-24 | 宁夏大学 | Process for extracting licoflavone, lycyrrhizic acid and licopolyose from liquorice root |
| CN1477104A (en) * | 2003-07-11 | 2004-02-25 | 上海奥利实业有限公司 | Extraction and purification method of licoflavone |
| CN1544429A (en) * | 2003-11-21 | 2004-11-10 | 长春中医学院 | Bee glue flavone extract preparation method, pharmaceutical preparation and its new medical uses |
-
2005
- 2005-11-16 CN CNB2005101195807A patent/CN100368408C/en not_active Expired - Fee Related
Patent Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1207910A (en) * | 1997-08-08 | 1999-02-17 | 中国科学院新疆化学研究所 | Licorice total flavone extracting method |
| CN1359905A (en) * | 2001-12-15 | 2002-07-24 | 宁夏大学 | Process for extracting licoflavone, lycyrrhizic acid and licopolyose from liquorice root |
| CN1477104A (en) * | 2003-07-11 | 2004-02-25 | 上海奥利实业有限公司 | Extraction and purification method of licoflavone |
| CN1544429A (en) * | 2003-11-21 | 2004-11-10 | 长春中医学院 | Bee glue flavone extract preparation method, pharmaceutical preparation and its new medical uses |
Non-Patent Citations (3)
| Title |
|---|
| 中草药. 乔仲和，胡自如，522-524，甘草渣仲大量黄酮类化合物的分离及甘草的综合开发研究. 1997 * |
| 兰州医学院学报. 封士兰，20-21，甘草黄酮的提取分离和含量测定. 1998 * |
| 石河子大学学报(自然科学版). 但建明等，427-428，甘草渣中黄酮类化合物的提取工艺研究. 2004 * |
Also Published As
| Publication number | Publication date |
|---|---|
| CN1752081A (en) | 2006-03-29 |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN102342359B (en) | Throat-soothing drop and preparation method thereof | |
| CN1994331A (en) | Super micronization method for botanical medicinal, product obtained thereby and use thereof | |
| CN100368408C (en) | Method of extracting licorice flavone from licorice slag | |
| CN107880145A (en) | A kind of new method that Siberian solomonseal rhizome polysaccharide is extracted from sealwort | |
| CN104306908A (en) | Health-care composition for preventing respiratory system diseases caused by haze, and preparation method thereof | |
| CN1272338C (en) | Licorice flavone extracting method from licorice dregs | |
| CN101829187A (en) | Preparation method of fruit-flavored cyclocarya paliurus chewable tablets | |
| CN102504038A (en) | Plant polysaccharide and preparation method thereof as well as application of plant polysaccharide in cigarette | |
| CN110384732B (en) | Traditional Chinese medicine composition, traditional Chinese medicine microecological preparation, and preparation method and application thereof | |
| CN104173700A (en) | Method for extracting total timosaponin | |
| CN101008022A (en) | Preparation method of scutellaria root flavone general aglycone extract | |
| Virk et al. | Lack of pharmacological basis of substitution of an endangered plant group Ashtawarga–A significant ingredient of polyherbal formulations | |
| CN105079467A (en) | Method for extracting anoectochilus roxburghii and application of extracts of anoectochilus roxburghii | |
| CN103690710A (en) | Pharmaceutical composition for treating gout and gouty arthritis | |
| CN104817601B (en) | Three flavones ingredient mixtures, preparation and its method are extracted from Herba lysimachiae capillipedis | |
| CN104127558A (en) | Heat-clearing and detoxifying traditional Chinese medicine composition for livestock, and preparation method and application thereof | |
| CN1052897C (en) | Chinese proprietary medicine for curing cold | |
| CN102552417B (en) | Traditional Chinese medicine for bird respiratory diseases | |
| CN102895303A (en) | Resin purification method for extracting and separating total flavone of cyclocarya paliurus | |
| CN111214535A (en) | A method for extracting Glycyrrhrizae radix extract from Glycyrrhrizae radix | |
| CN105175376A (en) | Method for extracting luteolin from Laifeng crossostephium leaves | |
| CN101703554A (en) | Preparation and use of semen cuscutae flavonoids | |
| AU2020101090A4 (en) | A Method for Preparing Flavonoids in Functional Passion Fruit Shells | |
| CN1102148C (en) | Preparation method of scutellaria root extract | |
| CN111690498A (en) | Guiling health-preserving medicinal liquor and preparation method thereof |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| C06 | Publication | ||
| PB01 | Publication | ||
| C10 | Entry into substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| C14 | Grant of patent or utility model | ||
| GR01 | Patent grant | ||
| C57 | Notification of unclear or unknown address | ||
| DD01 | Delivery of document by public notice |
Addressee: Li Bing Document name: Notification of Passing Examination on Formalities |
|
| DD01 | Delivery of document by public notice |
Addressee: Li Bing Document name: Notification to Pay the Fees |
|
| DD01 | Delivery of document by public notice |
Addressee: Li Bing Document name: Notification of Termination of Patent Right |
|
| C17 | Cessation of patent right | ||
| CF01 | Termination of patent right due to non-payment of annual fee |
Granted publication date: 20080213 Termination date: 20101116 |