CN100347280C - Cleaner for hemocyte analyzer and its prepn - Google Patents
Cleaner for hemocyte analyzer and its prepn Download PDFInfo
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- CN100347280C CN100347280C CNB031168507A CN03116850A CN100347280C CN 100347280 C CN100347280 C CN 100347280C CN B031168507 A CNB031168507 A CN B031168507A CN 03116850 A CN03116850 A CN 03116850A CN 100347280 C CN100347280 C CN 100347280C
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- clean
- out system
- cleaning agent
- agent
- blood cell
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- Apparatus For Disinfection Or Sterilisation (AREA)
Abstract
The present invention discloses a cleaning agent for a blood cell analyzer and a preparation method thereof. The components and the content of the cleaning agent for a blood cell analyzer comprises 3 to 9 ml/L of hydrolysis proteinase, 5 to 15 g/L of sodium formate, 4 to 12 g/L of sodium chloride and 15 to 25 ml/L of ampholytic active agent, wherein the pH of the cleaning agent is equal to 9.0 to 9.5. The preparation method comprises the following steps: the ampholytic active agent is heated and dissolved in the water and is cooled, the ampholytic active agent is sterilized and disinfected by ultra-violet lights and is added with sodium formate, sodium chloride, an anticorrosion agent, hydrolysis proteinase and a buffering agent of which the pH is equal to 9.0 to 9.5, and the cleaning agent can be obtained and is filled in dust-free and aseptic workshops. The cleaning agent has the advantages of easy preparation and strong dirt removing capacity; in addition, the cleaning agent not only is suitable for Kurt blood cell analyzers but also is suitable for blood cell analyzers, such as ABX, East Asia, Yapei, etc., and the cleaning agent has wide using prospects for the blood cell analyzers and has larger social benefits and economic benefits.
Description
Technical field
The present invention relates to a kind of clean-out system, relate in particular to a kind of clean-out system that is used for cellanalyzer and preparation method thereof.
Background technology
Cellanalyzer is a kind of analysis and detecting instrument of checking the patient blood situation, obtained using widely at hospital laboratory, the analytical data that cellanalyzer provided will be the important evidence of the diagnosis patient state of an illness, the accuracy of these data not only depends on the performance of instrument itself, also depends on the operation of this instrument simultaneously.Wherein, the cleaning before detecting will be an important step, and the clean-out system that is adopted when cleaning then is a crucial factor of cleaning performance.For this reason, numerous patents and document disclose technology separately, as: disclosed a kind of clean-out system as United States Patent (USP) 5985813, this clean-out system mainly is made of cats product, anion surfactant, nonionogenic tenside, ability of cleaning greasy dirt is stronger, but this clean-out system can not be eliminated the enzyme of protein residue thing; The clean-out system that company of U.S. Abbott Laboratories (ABBOTT) produces contains tensio-active agent and the subtilisin of eliminating the protein residue thing.This subtilisin is a kind of proteolytic enzyme that extracts from plant, costs an arm and a leg.
Summary of the invention
The technical issues that need to address of the present invention are to disclose a kind of cellanalyzer clean-out system, to overcome the above-mentioned defective that prior art exists, satisfy the needs of medical field;
Another technical problem that the present invention need solve provides the preparation method of above-mentioned clean-out system.
Technical scheme of the present invention:
Cellanalyzer clean-out system of the present invention is a kind of aqueous solution composition, and its component and content comprise:
Hydrolysising protease 3～9ml/L
Sodium formiate 5～15g/L
Sodium-chlor 4～12g/L
Amphoterics 15～25ml/L
The pH=9.0-9.5 of this clean-out system;
The hydrolysising protease of being addressed is a kind of lytic enzyme that comes from animal, described hydrolysising protease has the specificity to the blood of human body hydrolysis, substitute subtilisin with this proteolytic enzyme, not only can guarantee to remove bloodstain remaining in the cellanalyzer, and reduced cost, this hydrolysising protease can adopt the commercially available prod, is the hydrolysising protease of TtPe:EX Sayinase 16L as the trade mark;
The main effect of the sodium formiate of being addressed be can stabilized enzyme character;
The main effect of the sodium-chlor of being addressed is the specific conductivity of regulating the aqueous solution, makes clean-out system meet the electrical property of cellanalyzer;
The main effect of the amphoterics of being addressed is the dirt in dispersion and the emulsification instrument, can preferably adopt the trade mark is the zwitterionics of BS-12, and its chemical name is " trimethyl-glycine ", and this tensio-active agent soil removability is strong, and having certain germicidal action, adaptability is good.
According to optimized technical scheme of the present invention, also comprise an amount of sanitas, preferred add-on is 0.2～1ml/L, the mixture class of 5-chloro-2-methyl-4-isothiazoline-3-ketone and 2-methyl-4-isothiazoline-3-ketone preferably, the trade mark is CY-1.
The preparation method of clean-out system of the present invention comprises the steps:
According to said ratio, amphoterics is heated to 40～70 ℃ of dissolvings in water, be cooled to 10～30 ℃ then, with ultraviolet radiation for sterilizing and disinfecting 0.5～3 hour, the buffer reagent that adds sodium formiate, sodium-chlor, sanitas, hydrolysising protease, pH=9.0-9.5, promptly obtain clean-out system of the present invention, can in dustless, aseptic workshop.
The buffer reagent of being addressed can preferably adopt the buffering of three (methylol) amido methane right, its add-on with 1～5g/L for well.
Clean-out system of the present invention, preparation easily, soil removability is strong, not only be applicable to the Ku Erte cellanalyzer, and be applicable to the cellanalyzer of brands such as ABX, East Asia, Abbott Laboratories, for a kind of cellanalyzer clean-out system, have bigger social benefit and economic benefit with wide application prospect.
Embodiment
Embodiment 1
Place 1L water to be heated to 40 ℃ of dissolvings 20ml BS-12 amphoterics, be cooled to 30 ℃ then, with ultraviolet radiation for sterilizing and disinfecting 1 hour, the buffering of three (methylol) amido methane of adding 10 gram sodium formiates, 8 gram sodium-chlor, 0.5 gram CY-1 sanitas, 6ml hydrolysising protease, 1g pH=9.0-9.5 is right, stir, promptly obtain clean-out system of the present invention, can in dustless, aseptic workshop.
Embodiment 2
Adopt conventional method to be used for the cleaning of Ku Erte cellanalyzer the clean-out system of embodiment 1, the result shows that cleaning performance is very good, the noresidue bloodstain.
Claims (8)
1. a cellanalyzer clean-out system for a kind of aqueous solution composition, is characterized in that, its component and content comprise:
Animal hydrolyzed protein enzyme 3～9ml/L
Sodium formiate 5～15g/L
Sodium-chlor 4～12g/L
Amphoterics 15～25ml/L.
2. clean-out system according to claim 1 is characterized in that, also comprises an amount of sanitas.
3. clean-out system according to claim 1 is characterized in that the amphoterics of being addressed is a Varion CDG-K.
4. clean-out system according to claim 2 is characterized in that, the sanitas of being addressed is the mixture of 5-chloro-2-methyl-4-isothiazoline-3-ketone and 2-methyl-4-isothiazoline-3-ketone.
5. according to each described clean-out system of claim 1～4, it is characterized in that the pH=9.0-9.5 of clean-out system.
6. according to the preparation method of each described clean-out system of claim 1～5, it is characterized in that, comprise the steps:
With amphoterics heating for dissolving in water, cooling then, sterilization and disinfection adds sodium formiate, sodium-chlor, sanitas, hydrolysising protease, buffer reagent, promptly obtains clean-out system.
7. method according to claim 6 is characterized in that, the buffer reagent of being addressed adopts the buffering of three (methylol) amido methane right.
8. according to claim 6 or 7 described methods, it is characterized in that, amphoterics is heated to 40～70 ℃ of dissolvings in water, be cooled to 10～30 ℃ then, use ultraviolet radiation for sterilizing and disinfecting 0.5～3 hour.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CNB031168507A CN100347280C (en) | 2003-05-09 | 2003-05-09 | Cleaner for hemocyte analyzer and its prepn |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CNB031168507A CN100347280C (en) | 2003-05-09 | 2003-05-09 | Cleaner for hemocyte analyzer and its prepn |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN1548510A CN1548510A (en) | 2004-11-24 |
| CN100347280C true CN100347280C (en) | 2007-11-07 |
Family
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Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CNB031168507A Expired - Fee Related CN100347280C (en) | 2003-05-09 | 2003-05-09 | Cleaner for hemocyte analyzer and its prepn |
Country Status (1)
| Country | Link |
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| CN (1) | CN100347280C (en) |
Families Citing this family (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103773631B (en) * | 2014-02-12 | 2016-03-16 | 深圳市职业病防治院 | A kind of scavenging solution of blood coagulation analyzer |
| CN107557180A (en) * | 2017-09-26 | 2018-01-09 | 重庆赞龙生物科技有限公司 | A kind of cleaning fluid |
| CN108004052A (en) * | 2017-12-12 | 2018-05-08 | 山东兰桥医学科技有限公司 | A kind of blood analyser safe environment protection type cleaning agent |
| CN113004983A (en) * | 2019-12-20 | 2021-06-22 | 深圳市帝迈生物技术有限公司 | Cleaning solution, method for producing cleaning solution, and blood cell analyzer |
| CN113008653A (en) * | 2019-12-20 | 2021-06-22 | 深圳市帝迈生物技术有限公司 | Diluent, hemocyte analyzer, reagent for hemocyte analyzer, and kit |
| CN114686318A (en) * | 2022-03-28 | 2022-07-01 | 内江市第二人民医院 | Hemocyte analyzer cleaning solution and preparation method thereof |
Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US6043205A (en) * | 1997-10-01 | 2000-03-28 | Sysmex Corporation | Hematological analyzer sampling probe cleansing composition and method of using |
| JP2002323504A (en) * | 2001-04-25 | 2002-11-08 | Kanto Chem Co Inc | Alkaline cleaning liquid for clinical examination automatic analyzer |
-
2003
- 2003-05-09 CN CNB031168507A patent/CN100347280C/en not_active Expired - Fee Related
Patent Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US6043205A (en) * | 1997-10-01 | 2000-03-28 | Sysmex Corporation | Hematological analyzer sampling probe cleansing composition and method of using |
| JP2002323504A (en) * | 2001-04-25 | 2002-11-08 | Kanto Chem Co Inc | Alkaline cleaning liquid for clinical examination automatic analyzer |
Non-Patent Citations (2)
| Title |
|---|
| MET BC－2000血细胞分析仪稀释液、清洗液的研制及应用 麦穗.医学文选，第20卷第增刊期 2001 * |
| 用于血液与生化分析仪的糜蛋白酶清洁剂 陈则清等.现代医学仪器与应用，第9卷第4期 1997 * |
Also Published As
| Publication number | Publication date |
|---|---|
| CN1548510A (en) | 2004-11-24 |
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