CL2013001887A1 - Yarrowia lipolytica transformada que comprende un segmento de dna que codifica para el polipeptido qsuc2 de saccharomyces cerevisiae fusionado a la secuencia señal y preproregion xpr2 y a una porcion n-terminal de xor2. metodo para producir un compuesto de interes en base a dicha yarrowia. - Google Patents

Yarrowia lipolytica transformada que comprende un segmento de dna que codifica para el polipeptido qsuc2 de saccharomyces cerevisiae fusionado a la secuencia señal y preproregion xpr2 y a una porcion n-terminal de xor2. metodo para producir un compuesto de interes en base a dicha yarrowia.

Info

Publication number
CL2013001887A1
CL2013001887A1 CL2013001887A CL2013001887A CL2013001887A1 CL 2013001887 A1 CL2013001887 A1 CL 2013001887A1 CL 2013001887 A CL2013001887 A CL 2013001887A CL 2013001887 A CL2013001887 A CL 2013001887A CL 2013001887 A1 CL2013001887 A1 CL 2013001887A1
Authority
CL
Chile
Prior art keywords
yarrowia
qsuc2
preproregion
xpr2
xor2
Prior art date
Application number
CL2013001887A
Other languages
English (en)
Inventor
Quinn Qun Zhu
Seung-Pyo Hong
John E Seip
Original Assignee
Du Pont
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Du Pont filed Critical Du Pont
Publication of CL2013001887A1 publication Critical patent/CL2013001887A1/es

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
    • C12N1/14—Fungi; Culture media therefor
    • C12N1/16—Yeasts; Culture media therefor
    • C12N1/18—Baker's yeast; Brewer's yeast
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/24—Hydrolases (3) acting on glycosyl compounds (3.2)
    • C12N9/2402—Hydrolases (3) acting on glycosyl compounds (3.2) hydrolysing O- and S- glycosyl compounds (3.2.1)
    • C12N9/2405—Glucanases
    • C12N9/2408—Glucanases acting on alpha -1,4-glucosidic bonds
    • C12N9/2431—Beta-fructofuranosidase (3.2.1.26), i.e. invertase
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
    • C12N15/62—DNA sequences coding for fusion proteins
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/24—Hydrolases (3) acting on glycosyl compounds (3.2)
    • C12N9/2402—Hydrolases (3) acting on glycosyl compounds (3.2) hydrolysing O- and S- glycosyl compounds (3.2.1)
    • C12N9/2405—Glucanases
    • C12N9/2408—Glucanases acting on alpha -1,4-glucosidic bonds
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/48—Hydrolases (3) acting on peptide bonds (3.4)
    • C12N9/50—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25)
    • C12N9/58—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from fungi
    • C12N9/60—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from fungi from yeast
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P7/00—Preparation of oxygen-containing organic compounds
    • C12P7/64—Fats; Fatty oils; Ester-type waxes; Higher fatty acids, i.e. having at least seven carbon atoms in an unbroken chain bound to a carboxyl group; Oxidised oils or fats
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y302/00—Hydrolases acting on glycosyl compounds, i.e. glycosylases (3.2)
    • C12Y302/01—Glycosidases, i.e. enzymes hydrolysing O- and S-glycosyl compounds (3.2.1)
    • C12Y302/01026—Beta-fructofuranosidase (3.2.1.26), i.e. invertase
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2319/00—Fusion polypeptide
    • C07K2319/01—Fusion polypeptide containing a localisation/targetting motif
    • C07K2319/02—Fusion polypeptide containing a localisation/targetting motif containing a signal sequence

Landscapes

  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Organic Chemistry (AREA)
  • Engineering & Computer Science (AREA)
  • Genetics & Genomics (AREA)
  • Wood Science & Technology (AREA)
  • Zoology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Biotechnology (AREA)
  • Biochemistry (AREA)
  • Microbiology (AREA)
  • Biomedical Technology (AREA)
  • Mycology (AREA)
  • Molecular Biology (AREA)
  • Medicinal Chemistry (AREA)
  • Oil, Petroleum & Natural Gas (AREA)
  • General Chemical & Material Sciences (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Botany (AREA)
  • Virology (AREA)
  • Tropical Medicine & Parasitology (AREA)
  • Physics & Mathematics (AREA)
  • Biophysics (AREA)
  • Plant Pathology (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
CL2013001887A 2010-12-30 2013-06-26 Yarrowia lipolytica transformada que comprende un segmento de dna que codifica para el polipeptido qsuc2 de saccharomyces cerevisiae fusionado a la secuencia señal y preproregion xpr2 y a una porcion n-terminal de xor2. metodo para producir un compuesto de interes en base a dicha yarrowia. CL2013001887A1 (es)

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
US201061428590P 2010-12-30 2010-12-30

Publications (1)

Publication Number Publication Date
CL2013001887A1 true CL2013001887A1 (es) 2014-01-31

Family

ID=45063228

Family Applications (1)

Application Number Title Priority Date Filing Date
CL2013001887A CL2013001887A1 (es) 2010-12-30 2013-06-26 Yarrowia lipolytica transformada que comprende un segmento de dna que codifica para el polipeptido qsuc2 de saccharomyces cerevisiae fusionado a la secuencia señal y preproregion xpr2 y a una porcion n-terminal de xor2. metodo para producir un compuesto de interes en base a dicha yarrowia.

Country Status (10)

Country Link
US (1) US8735137B2 (es)
EP (1) EP2658962A1 (es)
JP (1) JP2014503216A (es)
KR (1) KR20140000711A (es)
CN (1) CN103443266A (es)
AU (1) AU2011353002A1 (es)
BR (1) BR112013016769A2 (es)
CA (1) CA2824584A1 (es)
CL (1) CL2013001887A1 (es)
WO (1) WO2012091812A1 (es)

Families Citing this family (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN104178435A (zh) * 2014-08-21 2014-12-03 天津科技大学 一种适合于高糖面团发酵的高活性干酵母
AU2016361425B2 (en) * 2015-11-24 2021-04-01 Cargill Incorporated Genetically modified yeasts and fermentation processes using genetically modified yeasts
CN107022499A (zh) * 2017-05-04 2017-08-08 北京化工大学 调控酿酒酵母社会行为提高生物乙醇发酵速率效率的方法
CN108070579A (zh) * 2017-12-21 2018-05-25 中诺生物科技发展江苏有限公司 一种蔗糖转化酶工业化生产方法
CN111996132A (zh) * 2020-07-21 2020-11-27 郑州大学 一种解脂耶氏酵母表面展示方法

Family Cites Families (39)

* Cited by examiner, † Cited by third party
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US5071764A (en) 1983-10-06 1991-12-10 Pfizer Inc. Process for integrative transformation of yarrowia lipolytica
US4880741A (en) 1983-10-06 1989-11-14 Pfizer Inc. Process for transformation of Yarrowia lipolytica
US4683202A (en) 1985-03-28 1987-07-28 Cetus Corporation Process for amplifying nucleic acid sequences
US4937189A (en) * 1985-10-18 1990-06-26 Pfizer Inc. Expression and secretion of heterologous proteins by Yarrowia lipolytica transformants
FR2626584B1 (fr) * 1988-01-28 1990-07-13 Agronomique Inst Nat Rech Sequence ars efficace chez yarrowia lipolytica et procede pour sa preparation
EP0402226A1 (en) 1989-06-06 1990-12-12 Institut National De La Recherche Agronomique Transformation vectors for yeast yarrowia
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US7259255B2 (en) 2003-06-25 2007-08-21 E. I. Du Pont De Nemours And Company Glyceraldehyde-3-phosphate dehydrogenase and phosphoglycerate mutase promoters for gene expression in oleaginous yeast
US20110059496A1 (en) 2003-06-25 2011-03-10 E. I. Du Pont De Nemours And Company Glyceraldehyde-3-phosphate dehydrogenase and phosphoglycerate mutase promoters for gene expression in oleaginous yeast
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US7202356B2 (en) 2003-11-14 2007-04-10 E. I. Du Pont De Nemours And Company Fructose-bisphosphate aldolase regulatory sequences for gene expression in oleaginous yeast
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US20060094102A1 (en) 2004-11-04 2006-05-04 Zhixiong Xue Ammonium transporter promoter for gene expression in oleaginous yeast
US7550286B2 (en) 2004-11-04 2009-06-23 E. I. Du Pont De Nemours And Company Docosahexaenoic acid producing strains of Yarrowia lipolytica
US7879591B2 (en) 2004-11-04 2011-02-01 E.I. Du Pont De Nemours And Company High eicosapentaenoic acid producing strains of Yarrowia lipolytica
WO2006125000A2 (en) 2005-05-19 2006-11-23 E.I. Du Pont De Nemours And Company Method for the production of resveratrol in a recombinant oleaginous microorganism
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US7678560B2 (en) 2006-05-17 2010-03-16 E.I. Du Pont De Nemours And Company Δ 5 desaturase and its use in making polyunsaturated fatty acids
US8367395B2 (en) 2006-09-28 2013-02-05 Dsm Ip Assets B.V. Production of sterols in oleaginous yeast and fungi
CA2663807C (en) 2006-10-30 2018-05-01 E.I. Du Pont De Nemours And Company .delta.17 desaturase and its use in making polyunsaturated fatty acids
US7709239B2 (en) 2006-12-07 2010-05-04 E.I. Du Pont De Nemours And Company Mutant Δ8 desaturase genes engineered by targeted mutagenesis and their use in making polyunsaturated fatty acids
US8846374B2 (en) 2006-12-12 2014-09-30 E I Du Pont De Nemours And Company Carotenoid production in a recombinant oleaginous yeast
WO2008124048A2 (en) 2007-04-03 2008-10-16 E. I. Du Pont De Nemours And Company Multizymes and their use in making polyunsaturated fatty acids
US7790156B2 (en) 2007-04-10 2010-09-07 E. I. Du Pont De Nemours And Company Δ-8 desaturases and their use in making polyunsaturated fatty acids
US7794701B2 (en) 2007-04-16 2010-09-14 E.I. Du Pont De Nemours And Company Δ-9 elongases and their use in making polyunsaturated fatty acids
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US8815567B2 (en) 2007-11-30 2014-08-26 E I Du Pont De Nemours And Company Coenzyme Q10 production in a recombinant oleaginous yeast
EP2294193A4 (en) 2008-04-10 2012-11-28 Dsm Ip Assets Bv PRODUCTION OF CAROTENOIDS IN YEAST AND OLEAGINOUS FUNGI
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CN102257126B (zh) 2008-12-18 2014-07-09 纳幕尔杜邦公司 减少发酵过程中的丙二酸副产物
JP2012530182A (ja) 2009-06-16 2012-11-29 イー・アイ・デュポン・ドウ・ヌムール・アンド・カンパニー 改良されたヤロウイア・リポリティカ（ｙａｒｒｏｗｉａｌｉｐｏｌｙｔｉｃａ）最適化株による高エイコサペンタエン酸油
US8637298B2 (en) 2009-06-16 2014-01-28 E I Du Pont De Nemours And Company Optimized strains of yarrowia lipolytica for high eicosapentaenoic acid production
CN103189513A (zh) 2010-03-31 2013-07-03 纳幕尔杜邦公司 上调磷酸戊糖途径以提高转基因微生物中受关注的非天然产物的产量

Also Published As

Publication number Publication date
JP2014503216A (ja) 2014-02-13
WO2012091812A1 (en) 2012-07-05
AU2011353002A1 (en) 2013-05-30
BR112013016769A2 (pt) 2016-10-11
EP2658962A1 (en) 2013-11-06
KR20140000711A (ko) 2014-01-03
US20120171719A1 (en) 2012-07-05
US8735137B2 (en) 2014-05-27
CN103443266A (zh) 2013-12-11
CA2824584A1 (en) 2012-07-05

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