CL2013001887A1 - Yarrowia lipolytica transformada que comprende un segmento de dna que codifica para el polipeptido qsuc2 de saccharomyces cerevisiae fusionado a la secuencia señal y preproregion xpr2 y a una porcion n-terminal de xor2. metodo para producir un compuesto de interes en base a dicha yarrowia. - Google Patents
Yarrowia lipolytica transformada que comprende un segmento de dna que codifica para el polipeptido qsuc2 de saccharomyces cerevisiae fusionado a la secuencia señal y preproregion xpr2 y a una porcion n-terminal de xor2. metodo para producir un compuesto de interes en base a dicha yarrowia.Info
- Publication number
- CL2013001887A1 CL2013001887A1 CL2013001887A CL2013001887A CL2013001887A1 CL 2013001887 A1 CL2013001887 A1 CL 2013001887A1 CL 2013001887 A CL2013001887 A CL 2013001887A CL 2013001887 A CL2013001887 A CL 2013001887A CL 2013001887 A1 CL2013001887 A1 CL 2013001887A1
- Authority
- CL
- Chile
- Prior art keywords
- yarrowia
- qsuc2
- preproregion
- xpr2
- xor2
- Prior art date
Links
- 108010076504 Protein Sorting Signals Proteins 0.000 title 1
- 240000004808 Saccharomyces cerevisiae Species 0.000 title 1
- 235000014680 Saccharomyces cerevisiae Nutrition 0.000 title 1
- 241000235013 Yarrowia Species 0.000 title 1
- 241000235015 Yarrowia lipolytica Species 0.000 title 1
- 150000001875 compounds Chemical class 0.000 title 1
- 238000004519 manufacturing process Methods 0.000 title 1
- 229920001184 polypeptide Polymers 0.000 title 1
- 108090000765 processed proteins & peptides Proteins 0.000 title 1
- 102000004196 processed proteins & peptides Human genes 0.000 title 1
Classifications
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
- C12N1/14—Fungi; Culture media therefor
- C12N1/16—Yeasts; Culture media therefor
- C12N1/18—Baker's yeast; Brewer's yeast
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
- C12N9/14—Hydrolases (3)
- C12N9/24—Hydrolases (3) acting on glycosyl compounds (3.2)
- C12N9/2402—Hydrolases (3) acting on glycosyl compounds (3.2) hydrolysing O- and S- glycosyl compounds (3.2.1)
- C12N9/2405—Glucanases
- C12N9/2408—Glucanases acting on alpha -1,4-glucosidic bonds
- C12N9/2431—Beta-fructofuranosidase (3.2.1.26), i.e. invertase
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
- C12N15/62—DNA sequences coding for fusion proteins
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
- C12N9/14—Hydrolases (3)
- C12N9/24—Hydrolases (3) acting on glycosyl compounds (3.2)
- C12N9/2402—Hydrolases (3) acting on glycosyl compounds (3.2) hydrolysing O- and S- glycosyl compounds (3.2.1)
- C12N9/2405—Glucanases
- C12N9/2408—Glucanases acting on alpha -1,4-glucosidic bonds
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
- C12N9/14—Hydrolases (3)
- C12N9/48—Hydrolases (3) acting on peptide bonds (3.4)
- C12N9/50—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25)
- C12N9/58—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from fungi
- C12N9/60—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from fungi from yeast
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
- C12P7/00—Preparation of oxygen-containing organic compounds
- C12P7/64—Fats; Fatty oils; Ester-type waxes; Higher fatty acids, i.e. having at least seven carbon atoms in an unbroken chain bound to a carboxyl group; Oxidised oils or fats
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Y—ENZYMES
- C12Y302/00—Hydrolases acting on glycosyl compounds, i.e. glycosylases (3.2)
- C12Y302/01—Glycosidases, i.e. enzymes hydrolysing O- and S-glycosyl compounds (3.2.1)
- C12Y302/01026—Beta-fructofuranosidase (3.2.1.26), i.e. invertase
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K2319/00—Fusion polypeptide
- C07K2319/01—Fusion polypeptide containing a localisation/targetting motif
- C07K2319/02—Fusion polypeptide containing a localisation/targetting motif containing a signal sequence
Landscapes
- Life Sciences & Earth Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Health & Medical Sciences (AREA)
- Organic Chemistry (AREA)
- Engineering & Computer Science (AREA)
- Genetics & Genomics (AREA)
- Wood Science & Technology (AREA)
- Zoology (AREA)
- Bioinformatics & Cheminformatics (AREA)
- General Engineering & Computer Science (AREA)
- General Health & Medical Sciences (AREA)
- Biotechnology (AREA)
- Biochemistry (AREA)
- Microbiology (AREA)
- Biomedical Technology (AREA)
- Mycology (AREA)
- Molecular Biology (AREA)
- Medicinal Chemistry (AREA)
- Oil, Petroleum & Natural Gas (AREA)
- General Chemical & Material Sciences (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Botany (AREA)
- Virology (AREA)
- Tropical Medicine & Parasitology (AREA)
- Physics & Mathematics (AREA)
- Biophysics (AREA)
- Plant Pathology (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
- Preparation Of Compounds By Using Micro-Organisms (AREA)
Applications Claiming Priority (1)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
US201061428590P | 2010-12-30 | 2010-12-30 |
Publications (1)
Publication Number | Publication Date |
---|---|
CL2013001887A1 true CL2013001887A1 (es) | 2014-01-31 |
Family
ID=45063228
Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
CL2013001887A CL2013001887A1 (es) | 2010-12-30 | 2013-06-26 | Yarrowia lipolytica transformada que comprende un segmento de dna que codifica para el polipeptido qsuc2 de saccharomyces cerevisiae fusionado a la secuencia señal y preproregion xpr2 y a una porcion n-terminal de xor2. metodo para producir un compuesto de interes en base a dicha yarrowia. |
Country Status (10)
Country | Link |
---|---|
US (1) | US8735137B2 (es) |
EP (1) | EP2658962A1 (es) |
JP (1) | JP2014503216A (es) |
KR (1) | KR20140000711A (es) |
CN (1) | CN103443266A (es) |
AU (1) | AU2011353002A1 (es) |
BR (1) | BR112013016769A2 (es) |
CA (1) | CA2824584A1 (es) |
CL (1) | CL2013001887A1 (es) |
WO (1) | WO2012091812A1 (es) |
Families Citing this family (5)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
CN104178435A (zh) * | 2014-08-21 | 2014-12-03 | 天津科技大学 | 一种适合于高糖面团发酵的高活性干酵母 |
AU2016361425B2 (en) * | 2015-11-24 | 2021-04-01 | Cargill Incorporated | Genetically modified yeasts and fermentation processes using genetically modified yeasts |
CN107022499A (zh) * | 2017-05-04 | 2017-08-08 | 北京化工大学 | 调控酿酒酵母社会行为提高生物乙醇发酵速率效率的方法 |
CN108070579A (zh) * | 2017-12-21 | 2018-05-25 | 中诺生物科技发展江苏有限公司 | 一种蔗糖转化酶工业化生产方法 |
CN111996132A (zh) * | 2020-07-21 | 2020-11-27 | 郑州大学 | 一种解脂耶氏酵母表面展示方法 |
Family Cites Families (39)
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US5071764A (en) | 1983-10-06 | 1991-12-10 | Pfizer Inc. | Process for integrative transformation of yarrowia lipolytica |
US4880741A (en) | 1983-10-06 | 1989-11-14 | Pfizer Inc. | Process for transformation of Yarrowia lipolytica |
US4683202A (en) | 1985-03-28 | 1987-07-28 | Cetus Corporation | Process for amplifying nucleic acid sequences |
US4937189A (en) * | 1985-10-18 | 1990-06-26 | Pfizer Inc. | Expression and secretion of heterologous proteins by Yarrowia lipolytica transformants |
FR2626584B1 (fr) * | 1988-01-28 | 1990-07-13 | Agronomique Inst Nat Rech | Sequence ars efficace chez yarrowia lipolytica et procede pour sa preparation |
EP0402226A1 (en) | 1989-06-06 | 1990-12-12 | Institut National De La Recherche Agronomique | Transformation vectors for yeast yarrowia |
US7238482B2 (en) | 2003-05-07 | 2007-07-03 | E. I. Du Pont De Nemours And Company | Production of polyunsaturated fatty acids in oleaginous yeasts |
US7125672B2 (en) | 2003-05-07 | 2006-10-24 | E. I. Du Pont De Nemours And Company | Codon-optimized genes for the production of polyunsaturated fatty acids in oleaginous yeasts |
US7259255B2 (en) | 2003-06-25 | 2007-08-21 | E. I. Du Pont De Nemours And Company | Glyceraldehyde-3-phosphate dehydrogenase and phosphoglycerate mutase promoters for gene expression in oleaginous yeast |
US20110059496A1 (en) | 2003-06-25 | 2011-03-10 | E. I. Du Pont De Nemours And Company | Glyceraldehyde-3-phosphate dehydrogenase and phosphoglycerate mutase promoters for gene expression in oleaginous yeast |
US7504259B2 (en) | 2003-11-12 | 2009-03-17 | E. I. Du Pont De Nemours And Company | Δ12 desaturases suitable for altering levels of polyunsaturated fatty acids in oleaginous yeast |
US7202356B2 (en) | 2003-11-14 | 2007-04-10 | E. I. Du Pont De Nemours And Company | Fructose-bisphosphate aldolase regulatory sequences for gene expression in oleaginous yeast |
WO2006012326A1 (en) | 2004-06-25 | 2006-02-02 | E.I. Dupont De Nemours And Company | Delta-8 desaturase and its use in making polyunsaturated fatty acids |
US20060094102A1 (en) | 2004-11-04 | 2006-05-04 | Zhixiong Xue | Ammonium transporter promoter for gene expression in oleaginous yeast |
US7550286B2 (en) | 2004-11-04 | 2009-06-23 | E. I. Du Pont De Nemours And Company | Docosahexaenoic acid producing strains of Yarrowia lipolytica |
US7879591B2 (en) | 2004-11-04 | 2011-02-01 | E.I. Du Pont De Nemours And Company | High eicosapentaenoic acid producing strains of Yarrowia lipolytica |
WO2006125000A2 (en) | 2005-05-19 | 2006-11-23 | E.I. Du Pont De Nemours And Company | Method for the production of resveratrol in a recombinant oleaginous microorganism |
US7470532B2 (en) | 2005-10-19 | 2008-12-30 | E.I. Du Pont De Nemours And Company | Mortierella alpina C16/18 fatty acid elongase |
AU2006318738B2 (en) | 2005-11-23 | 2011-12-22 | E. I. Du Pont De Nemours And Company | Delta-9 elongases and their use in making polyunsaturated fatty acids |
EP2004801A2 (en) | 2006-03-20 | 2008-12-24 | Microbia Precision Engineering | Production of quinone derived compounds in oleaginous yeast fungi |
US7695950B2 (en) | 2006-05-17 | 2010-04-13 | E. I. Du Pont De Nemours And Company | Δ5 desaturase and its use in making polyunsaturated fatty acids |
US7678560B2 (en) | 2006-05-17 | 2010-03-16 | E.I. Du Pont De Nemours And Company | Δ 5 desaturase and its use in making polyunsaturated fatty acids |
US8367395B2 (en) | 2006-09-28 | 2013-02-05 | Dsm Ip Assets B.V. | Production of sterols in oleaginous yeast and fungi |
CA2663807C (en) | 2006-10-30 | 2018-05-01 | E.I. Du Pont De Nemours And Company | .delta.17 desaturase and its use in making polyunsaturated fatty acids |
US7709239B2 (en) | 2006-12-07 | 2010-05-04 | E.I. Du Pont De Nemours And Company | Mutant Δ8 desaturase genes engineered by targeted mutagenesis and their use in making polyunsaturated fatty acids |
US8846374B2 (en) | 2006-12-12 | 2014-09-30 | E I Du Pont De Nemours And Company | Carotenoid production in a recombinant oleaginous yeast |
WO2008124048A2 (en) | 2007-04-03 | 2008-10-16 | E. I. Du Pont De Nemours And Company | Multizymes and their use in making polyunsaturated fatty acids |
US7790156B2 (en) | 2007-04-10 | 2010-09-07 | E. I. Du Pont De Nemours And Company | Δ-8 desaturases and their use in making polyunsaturated fatty acids |
US7794701B2 (en) | 2007-04-16 | 2010-09-14 | E.I. Du Pont De Nemours And Company | Δ-9 elongases and their use in making polyunsaturated fatty acids |
US7943365B2 (en) | 2007-05-03 | 2011-05-17 | E.I. Du Pont De Nemours And Company | Δ-5 desaturases and their use in making polyunsaturated fatty acids |
SG10201509864QA (en) * | 2007-06-01 | 2015-12-30 | Solazyme Inc | Production of oil in microorganisms |
US8323935B2 (en) | 2007-10-03 | 2012-12-04 | E I Du Pont De Nemours And Company | Optimized strains of Yarrowia lipolytica for high eicosapentaenoic acid production |
US8815567B2 (en) | 2007-11-30 | 2014-08-26 | E I Du Pont De Nemours And Company | Coenzyme Q10 production in a recombinant oleaginous yeast |
EP2294193A4 (en) | 2008-04-10 | 2012-11-28 | Dsm Ip Assets Bv | PRODUCTION OF CAROTENOIDS IN YEAST AND OLEAGINOUS FUNGI |
EP2324119B1 (en) | 2008-09-19 | 2016-03-30 | E. I. du Pont de Nemours and Company | Mutant delta5 desaturases and their use in making polyunsaturated fatty acids |
CN102257126B (zh) | 2008-12-18 | 2014-07-09 | 纳幕尔杜邦公司 | 减少发酵过程中的丙二酸副产物 |
JP2012530182A (ja) | 2009-06-16 | 2012-11-29 | イー・アイ・デュポン・ドウ・ヌムール・アンド・カンパニー | 改良されたヤロウイア・リポリティカ(yarrowialipolytica)最適化株による高エイコサペンタエン酸油 |
US8637298B2 (en) | 2009-06-16 | 2014-01-28 | E I Du Pont De Nemours And Company | Optimized strains of yarrowia lipolytica for high eicosapentaenoic acid production |
CN103189513A (zh) | 2010-03-31 | 2013-07-03 | 纳幕尔杜邦公司 | 上调磷酸戊糖途径以提高转基因微生物中受关注的非天然产物的产量 |
-
2011
- 2011-11-15 BR BR112013016769A patent/BR112013016769A2/pt not_active IP Right Cessation
- 2011-11-15 JP JP2013547474A patent/JP2014503216A/ja active Pending
- 2011-11-15 KR KR1020137019892A patent/KR20140000711A/ko not_active Application Discontinuation
- 2011-11-15 CN CN2011800630477A patent/CN103443266A/zh active Pending
- 2011-11-15 AU AU2011353002A patent/AU2011353002A1/en not_active Abandoned
- 2011-11-15 US US13/296,524 patent/US8735137B2/en not_active Expired - Fee Related
- 2011-11-15 CA CA2824584A patent/CA2824584A1/en not_active Abandoned
- 2011-11-15 EP EP11788953.5A patent/EP2658962A1/en not_active Withdrawn
- 2011-11-15 WO PCT/US2011/060788 patent/WO2012091812A1/en active Application Filing
-
2013
- 2013-06-26 CL CL2013001887A patent/CL2013001887A1/es unknown
Also Published As
Publication number | Publication date |
---|---|
JP2014503216A (ja) | 2014-02-13 |
WO2012091812A1 (en) | 2012-07-05 |
AU2011353002A1 (en) | 2013-05-30 |
BR112013016769A2 (pt) | 2016-10-11 |
EP2658962A1 (en) | 2013-11-06 |
KR20140000711A (ko) | 2014-01-03 |
US20120171719A1 (en) | 2012-07-05 |
US8735137B2 (en) | 2014-05-27 |
CN103443266A (zh) | 2013-12-11 |
CA2824584A1 (en) | 2012-07-05 |
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