CL2011001462A1 - Yarrowia sp. transgenica util en la fermentacion de acidos grasos poliinsaturados que comprende un polipeptido con actividad malonil-coa sintetasa; y metodo para reducir la produccion de malonatos como subproducto de la fermetacion basado en el uso de dicha yarrowia. - Google Patents

Yarrowia sp. transgenica util en la fermentacion de acidos grasos poliinsaturados que comprende un polipeptido con actividad malonil-coa sintetasa; y metodo para reducir la produccion de malonatos como subproducto de la fermetacion basado en el uso de dicha yarrowia.

Info

Publication number
CL2011001462A1
CL2011001462A1 CL2011001462A CL2011001462A CL2011001462A1 CL 2011001462 A1 CL2011001462 A1 CL 2011001462A1 CL 2011001462 A CL2011001462 A CL 2011001462A CL 2011001462 A CL2011001462 A CL 2011001462A CL 2011001462 A1 CL2011001462 A1 CL 2011001462A1
Authority
CL
Chile
Prior art keywords
yarrowia
fermentation
malonyl
malonates
byproduct
Prior art date
Application number
CL2011001462A
Other languages
English (en)
Inventor
Zhixiong Xue
Quinn Qun Zhu
Original Assignee
Du Pont
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Du Pont filed Critical Du Pont
Publication of CL2011001462A1 publication Critical patent/CL2011001462A1/es

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P7/00—Preparation of oxygen-containing organic compounds
    • C12P7/64—Fats; Fatty oils; Ester-type waxes; Higher fatty acids, i.e. having at least seven carbon atoms in an unbroken chain bound to a carboxyl group; Oxidised oils or fats
    • C12P7/6409—Fatty acids
    • C12P7/6427—Polyunsaturated fatty acids [PUFA], i.e. having two or more double bonds in their backbone
    • C12P7/6432—Eicosapentaenoic acids [EPA]
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/93—Ligases (6)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P7/00—Preparation of oxygen-containing organic compounds
    • C12P7/64—Fats; Fatty oils; Ester-type waxes; Higher fatty acids, i.e. having at least seven carbon atoms in an unbroken chain bound to a carboxyl group; Oxidised oils or fats
    • C12P7/6436—Fatty acid esters
    • C12P7/6445—Glycerides
    • C12P7/6472—Glycerides containing polyunsaturated fatty acid [PUFA] residues, i.e. having two or more double bonds in their backbone

Landscapes

  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Zoology (AREA)
  • Engineering & Computer Science (AREA)
  • Wood Science & Technology (AREA)
  • Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Microbiology (AREA)
  • Biotechnology (AREA)
  • Biochemistry (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Oil, Petroleum & Natural Gas (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • General Chemical & Material Sciences (AREA)
  • Biomedical Technology (AREA)
  • Molecular Biology (AREA)
  • Medicinal Chemistry (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)

Abstract

Organismo transgénico porque comprende un gen de la malonil-coa sintetasa; método para manipular el contenido de malonatos en un organismo transgénico; célula transgénica de yarrowia sp.
CL2011001462A 2008-12-18 2011-06-16 Yarrowia sp. transgenica util en la fermentacion de acidos grasos poliinsaturados que comprende un polipeptido con actividad malonil-coa sintetasa; y metodo para reducir la produccion de malonatos como subproducto de la fermetacion basado en el uso de dicha yarrowia. CL2011001462A1 (es)

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
US13892208P 2008-12-18 2008-12-18

Publications (1)

Publication Number Publication Date
CL2011001462A1 true CL2011001462A1 (es) 2012-03-30

Family

ID=41820237

Family Applications (1)

Application Number Title Priority Date Filing Date
CL2011001462A CL2011001462A1 (es) 2008-12-18 2011-06-16 Yarrowia sp. transgenica util en la fermentacion de acidos grasos poliinsaturados que comprende un polipeptido con actividad malonil-coa sintetasa; y metodo para reducir la produccion de malonatos como subproducto de la fermetacion basado en el uso de dicha yarrowia.

Country Status (9)

Country Link
US (1) US9175318B2 (es)
EP (1) EP2358862B1 (es)
JP (1) JP5620402B2 (es)
CN (1) CN102257126B (es)
AU (1) AU2009335903B2 (es)
BR (1) BRPI0917722A2 (es)
CA (1) CA2744716C (es)
CL (1) CL2011001462A1 (es)
WO (1) WO2010080388A1 (es)

Families Citing this family (19)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JP2012530182A (ja) * 2009-06-16 2012-11-29 イー・アイ・デュポン・ドウ・ヌムール・アンド・カンパニー 改良されたヤロウイア・リポリティカ（ｙａｒｒｏｗｉａｌｉｐｏｌｙｔｉｃａ）最適化株による高エイコサペンタエン酸油
CN102803289B (zh) 2009-06-16 2015-07-22 纳幕尔杜邦公司 用于高水平生产二十碳五烯酸的改善的优化的解脂耶氏酵母菌株
US8637298B2 (en) * 2009-06-16 2014-01-28 E I Du Pont De Nemours And Company Optimized strains of yarrowia lipolytica for high eicosapentaenoic acid production
AU2011242843A1 (en) 2010-04-22 2012-09-27 E. I. Du Pont De Nemours And Company Method for obtaining polyunsaturated fatty acid-containing compositions from microbial biomass
WO2012027689A1 (en) 2010-08-26 2012-03-01 E. I. Du Pont De Nemours And Company Recombinant microbial host cells for high eicosapentaenoic acid production
EP2609202A1 (en) 2010-08-26 2013-07-03 E.I. Du Pont De Nemours And Company Mutant hpgg motif and hdash motif delta-5 desaturases and their use in making polyunsaturated fatty acids
AU2011293167B2 (en) 2010-08-26 2016-12-08 E. I. Du Pont De Nemours And Company Mutant delta-9 elongases and their use in making polyunsaturated fatty acids
BR112013016769A2 (pt) 2010-12-30 2016-10-11 Du Pont yarrowia lipolytica transformada e método para produzir pelo menos um produto não nativo de interesse
US20130040340A1 (en) 2011-02-07 2013-02-14 E. I. Du Pont De Nemours And Company Production of alcohol esters in situ using alcohols and fatty acids produced by microorganisms
CA3026436A1 (en) 2011-02-11 2012-08-16 E. I. Du Pont De Nemours And Company Method for obtaining a lipid-containing composition from microbial biomass
BR112015014844A2 (pt) 2012-12-21 2019-01-15 Du Pont célula microbiana recombinante e método de produção de um óleo microbiano
DE102015202013B4 (de) 2015-02-05 2019-05-09 Adidas Ag Verfahren zur Herstellung eines Kunststoffformteils, Kunststoffformteil und Schuh
WO2016130597A1 (en) * 2015-02-10 2016-08-18 GeneSys Consulting, LLC Methylmalonic acid compositions, biological methods for making same, and microorganisms for making same
DE102016209045B4 (de) 2016-05-24 2022-05-25 Adidas Ag Verfahren und vorrichtung zum automatischen herstellen von schuhsohlen, sohlen und schuhe
DE102016209044B4 (de) 2016-05-24 2019-08-29 Adidas Ag Sohlenform zum Herstellen einer Sohle und Anordnung einer Vielzahl von Sohlenformen
DE102016209046B4 (de) 2016-05-24 2019-08-08 Adidas Ag Verfahren zur herstellung einer schuhsohle, schuhsohle, schuh und vorgefertigte tpu-gegenstände
DE102016223980B4 (de) 2016-12-01 2022-09-22 Adidas Ag Verfahren zur Herstellung eines Kunststoffformteils
DE102017205830B4 (de) 2017-04-05 2020-09-24 Adidas Ag Verfahren für die Nachbehandlung einer Vielzahl einzelner expandierter Partikel für die Herstellung mindestens eines Teils eines gegossenen Sportartikels, Sportartikel und Sportschuh
CN110063161B (zh) * 2019-03-20 2021-09-10 广州中医药大学(广州中医药研究院) 一种利用田菁茎瘤固氮根瘤菌促进穿心莲生长并提高其有效成分含量的方法

Family Cites Families (34)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4880741A (en) 1983-10-06 1989-11-14 Pfizer Inc. Process for transformation of Yarrowia lipolytica
US5071764A (en) 1983-10-06 1991-12-10 Pfizer Inc. Process for integrative transformation of yarrowia lipolytica
US4683202A (en) 1985-03-28 1987-07-28 Cetus Corporation Process for amplifying nucleic acid sequences
US5107065A (en) 1986-03-28 1992-04-21 Calgene, Inc. Anti-sense regulation of gene expression in plant cells
JP2527340B2 (ja) 1986-12-15 1996-08-21 アプライド バイオシステムズ インコーポレイテッド ロ―ダミン染料の５−及び６−スクシニミジルカルボキシレ―ト異性体
US5366860A (en) 1989-09-29 1994-11-22 Applied Biosystems, Inc. Spectrally resolvable rhodamine dyes for nucleic acid sequence determination
EP1034308A4 (en) 1997-12-04 2002-04-03 Smithkline Beecham Corp PROCESS USING ANTISENSE EXPRESSION SEQUENCES TO PRODUCE CONDITIONALLY EXPRESSED MUTANT CELLS
US6939691B1 (en) * 1999-10-13 2005-09-06 Board Of Trustees Of The Leland Stanford Junior University E. coli and Streptomyces host cells that contain MatBC genes or E. coli host cells that contain pcc genes useful for enhanced polyketide production
US6998256B2 (en) * 2000-04-28 2006-02-14 Kosan Biosciences, Inc. Methods of obtaining epothilone D using crystallization and /or by the culture of cells in the presence of methyl oleate
TWI350854B (en) 2001-04-16 2011-10-21 Martek Biosciences Corp Product and process for transformation of thraustochytriales microorganisms
US7125672B2 (en) 2003-05-07 2006-10-24 E. I. Du Pont De Nemours And Company Codon-optimized genes for the production of polyunsaturated fatty acids in oleaginous yeasts
US7238482B2 (en) 2003-05-07 2007-07-03 E. I. Du Pont De Nemours And Company Production of polyunsaturated fatty acids in oleaginous yeasts
US7214491B2 (en) 2003-05-07 2007-05-08 E. I. Du Pont De Nemours And Company Δ-12 desaturase gene suitable for altering levels of polyunsaturated fatty acids in oleaginous yeasts
US7259255B2 (en) 2003-06-25 2007-08-21 E. I. Du Pont De Nemours And Company Glyceraldehyde-3-phosphate dehydrogenase and phosphoglycerate mutase promoters for gene expression in oleaginous yeast
US7504259B2 (en) 2003-11-12 2009-03-17 E. I. Du Pont De Nemours And Company Δ12 desaturases suitable for altering levels of polyunsaturated fatty acids in oleaginous yeast
AU2004290051A1 (en) 2003-11-12 2005-05-26 E.I. Dupont De Nemours And Company Delta-15 desaturases suitable for altering levels of polyunsaturated fatty acids in oilseed plants and oleaginous yeast
WO2006012326A1 (en) 2004-06-25 2006-02-02 E.I. Dupont De Nemours And Company Delta-8 desaturase and its use in making polyunsaturated fatty acids
US7465564B2 (en) 2004-08-10 2008-12-16 E.I. Du Pont De Nemours And Company Production of γ-linolenic acid in oleaginous yeast
WO2006044290A2 (en) * 2004-10-12 2006-04-27 Board Of Trustees Of Michigan State University Biosynthesis of philoroglucinol and preparation of 1,3-dihydroxybenzene therefrom
US7617795B2 (en) 2004-10-13 2009-11-17 Embrex, Inc. Methods and apparatus for injecting and sampling material through avian egg membranes
US7550286B2 (en) 2004-11-04 2009-06-23 E. I. Du Pont De Nemours And Company Docosahexaenoic acid producing strains of Yarrowia lipolytica
DE102004062294A1 (de) * 2004-12-23 2006-07-06 Basf Plant Science Gmbh Verfahren zur Herstellung von mehrfach ungesättigten langkettigen Fettsäuren in transgenen Organismen
WO2006125000A2 (en) * 2005-05-19 2006-11-23 E.I. Du Pont De Nemours And Company Method for the production of resveratrol in a recombinant oleaginous microorganism
WO2006124999A2 (en) 2005-05-19 2006-11-23 E. I. Du Pont De Nemours And Company Method for the production of resveratrol in a recombinant bacterial host cell
US7470532B2 (en) 2005-10-19 2008-12-30 E.I. Du Pont De Nemours And Company Mortierella alpina C16/18 fatty acid elongase
AU2006318738B2 (en) 2005-11-23 2011-12-22 E. I. Du Pont De Nemours And Company Delta-9 elongases and their use in making polyunsaturated fatty acids
US7678560B2 (en) 2006-05-17 2010-03-16 E.I. Du Pont De Nemours And Company Δ 5 desaturase and its use in making polyunsaturated fatty acids
US7695950B2 (en) 2006-05-17 2010-04-13 E. I. Du Pont De Nemours And Company Δ5 desaturase and its use in making polyunsaturated fatty acids
CA2663807C (en) 2006-10-30 2018-05-01 E.I. Du Pont De Nemours And Company .delta.17 desaturase and its use in making polyunsaturated fatty acids
US7709239B2 (en) 2006-12-07 2010-05-04 E.I. Du Pont De Nemours And Company Mutant Δ8 desaturase genes engineered by targeted mutagenesis and their use in making polyunsaturated fatty acids
WO2008124048A2 (en) 2007-04-03 2008-10-16 E. I. Du Pont De Nemours And Company Multizymes and their use in making polyunsaturated fatty acids
US7794701B2 (en) * 2007-04-16 2010-09-14 E.I. Du Pont De Nemours And Company Δ-9 elongases and their use in making polyunsaturated fatty acids
US8323935B2 (en) 2007-10-03 2012-12-04 E I Du Pont De Nemours And Company Optimized strains of Yarrowia lipolytica for high eicosapentaenoic acid production
WO2009046248A1 (en) * 2007-10-03 2009-04-09 E. I. Du Pont De Nemours And Company Peroxisome biogenesis factor protein (pex) disruptions for altering the content of polyunsaturated fatty acids and the total lipid content in oleaginous eukaryotic organisms

Also Published As

Publication number Publication date
US9175318B2 (en) 2015-11-03
CA2744716C (en) 2017-05-23
US20100159558A1 (en) 2010-06-24
EP2358862A1 (en) 2011-08-24
WO2010080388A1 (en) 2010-07-15
AU2009335903B2 (en) 2016-04-14
EP2358862B1 (en) 2017-08-02
BRPI0917722A2 (pt) 2017-05-30
AU2009335903A1 (en) 2010-07-15
JP2012512656A (ja) 2012-06-07
CA2744716A1 (en) 2010-07-15
CN102257126A (zh) 2011-11-23
CN102257126B (zh) 2014-07-09
JP5620402B2 (ja) 2014-11-05

Similar Documents

Publication Publication Date Title
CL2011001462A1 (es) Yarrowia sp. transgenica util en la fermentacion de acidos grasos poliinsaturados que comprende un polipeptido con actividad malonil-coa sintetasa; y metodo para reducir la produccion de malonatos como subproducto de la fermetacion basado en el uso de dicha yarrowia.
UA101214C2 (ru) Гидролазы, кодирующеие их нуклеиновые кислоты и способы их получения и применения
AR065168A1 (es) Produccion de biodiesel
PH12013502032A1 (en) Microorganisms for producing methacrylic acid and methacrylate esters and methods related thereto
WO2011022229A3 (en) Method for enhanced sustainable production of algal bio-products, comprising use of symbiotic diazotroph-attenuated stress co-cultivation
CO6270374A2 (es) Metodos y composiciones para la produccion de aceites, combustible y oleoquimicos fabricados a`partir de microorganismos
AU2018256637A1 (en) Brassica rod1 gene sequences and uses thereof
SG179048A1 (en) Microorganisms and methods for the co-production of isopropanol with primary alcohols, diols and acids
PE20090740A1 (es) Modificacion enzimatica del aceite
CO6400153A2 (es) Producción de aceites específicos en microorganismos heterótrofos recombinantes
EA201171291A1 (ru) Кормовая добавка
GB2485112A (en) Differentiation of human embryonic stem cells
UA103335C2 (ru) Способ изготовления добавки для ферментативного расщепления микотоксинов, добавка и ее применение
EA201291332A1 (ru) Уменьшение содержания насыщенных жирных кислот в семенах растений
BR112015005782A2 (pt) variantes de enzima com propriedades aprimoradas de éster-sintase
CL2015001836A1 (es) Sistemas para procesar transesterificación/esterificación enzimática y procesos que emplean lipasas inmobilizadas en resinas hidrófóbicas
MX2012007184A (es) Acidos grasos de cadena ramificada y produccion biologica de estos.
HK1141044A1 (en) Production of biodiesel fuels which are low in glycerin and sulfur
EA020145B9 (ru) Гидролазы, кодирующие их нуклеиновые кислоты и способы их получения и применения
AR100382A1 (es) Método para producir nailon
MY161118A (en) Use of methanesulfonic acid for producing fatty acid esters
CL2011003163A1 (es) Celula huesped microbiana oleaginosa recombinante para la produccion mejorada de al menos un acido graso poliinsaturado de cadena larga que codifica un polipeptido con actividad acilcoa: lisofosfolipido; metodo para elaborar aceite que comprende acido eicosapentaenoico y/o acido docosahexaenoico.
CL2014003419A1 (es) Célula microbiana recombinante que sobreexpresa una secuencia que codifica acil-coa: lisofosfatidilcolina aciltranferasa y fosofolípido: diacilglicerol aciltransferasas; y método de producción mejorada de ácidos grasos poliinsaturados.
MY152746A (en) Altering enzyme balance through fermentation conditions
BRPI0911286A8 (pt) Método aperfeiçoado de produção de biodisel