CL2009000193A1 - Vector para la transferencia de genes capaz de reproducirse en plantas que contienen genes del virus lr-2, celula vegetal que lo comprende, metodo de produccion de dicha construccion molecular, y procedimiento para expresar un gen heterologo en una celula vegetal. - Google Patents
Vector para la transferencia de genes capaz de reproducirse en plantas que contienen genes del virus lr-2, celula vegetal que lo comprende, metodo de produccion de dicha construccion molecular, y procedimiento para expresar un gen heterologo en una celula vegetal.Info
- Publication number
- CL2009000193A1 CL2009000193A1 CL2009000193A CL2009000193A CL2009000193A1 CL 2009000193 A1 CL2009000193 A1 CL 2009000193A1 CL 2009000193 A CL2009000193 A CL 2009000193A CL 2009000193 A CL2009000193 A CL 2009000193A CL 2009000193 A1 CL2009000193 A1 CL 2009000193A1
- Authority
- CL
- Chile
- Prior art keywords
- vector
- plant cell
- genes
- virus
- cell
- Prior art date
Links
Classifications
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- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
- C07K14/005—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from viruses
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
- C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
- C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
- C12N15/8216—Methods for controlling, regulating or enhancing expression of transgenes in plant cells
- C12N15/8237—Externally regulated expression systems
- C12N15/8239—Externally regulated expression systems pathogen inducible
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
- C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
- C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
- C12N15/8201—Methods for introducing genetic material into plant cells, e.g. DNA, RNA, stable or transient incorporation, tissue culture methods adapted for transformation
- C12N15/8202—Methods for introducing genetic material into plant cells, e.g. DNA, RNA, stable or transient incorporation, tissue culture methods adapted for transformation by biological means, e.g. cell mediated or natural vector
- C12N15/8203—Virus mediated transformation
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N2770/00—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA ssRNA viruses positive-sense
- C12N2770/00011—Details
- C12N2770/00022—New viral proteins or individual genes, new structural or functional aspects of known viral proteins or genes
Landscapes
- Health & Medical Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Genetics & Genomics (AREA)
- Engineering & Computer Science (AREA)
- Chemical & Material Sciences (AREA)
- Organic Chemistry (AREA)
- Biomedical Technology (AREA)
- Biotechnology (AREA)
- Molecular Biology (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Zoology (AREA)
- General Engineering & Computer Science (AREA)
- Wood Science & Technology (AREA)
- Biochemistry (AREA)
- Biophysics (AREA)
- General Health & Medical Sciences (AREA)
- Virology (AREA)
- Plant Pathology (AREA)
- Physics & Mathematics (AREA)
- Cell Biology (AREA)
- Microbiology (AREA)
- Medicinal Chemistry (AREA)
- Gastroenterology & Hepatology (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Communicable Diseases (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
- Breeding Of Plants And Reproduction By Means Of Culturing (AREA)
Abstract
UN VECTOR PARA LA EXPRESION DE GENES CAPAZ DE REPLICARSE EN VIDES QUE COMPRENDE UN ÁCIDO NUCLEICO QUE CODIFICA: A) GENES VIRALES DEL GRAPEVINE LEAFROL-ASSOCIATED VIRUS-2 (LR-2) COMPRENDIENDO UNA METILTRANSFERASA, UNA ARN HELICASA, Y UNA ARN POLMERASA DEPENDIENTE DE ARN; B) UN ARNI SUPRESOR SELECCIONADO DE) GRUPO FORMADO POR P24 DE LR-2 Y P21 DEL VIRUS AMARILLO DE LA REMOLACHA; C) PROTEASAS DIRECTRICES L1 Y L2 DE LR-2; Y D) UN POLINUCLEÁTIDO HETERÓLOGO UNIDO OPERATIVAMENTE A UN PROMOTOR. UNA CÉLULA DE VID QUE COMPRENDE DICHO VECTOR. UN MÉTODO PARA EXPRESAR UN GEN HETERÓLOGO EN UNA CÉLULA DE VID QUE COMPRENDE INTRODUCIR DICHO VECTOR EN LA CÉLULA DE VID. UN MÉTODO PARA PRODUCIR DICHO VECTOR QUE ADEMÁS COMPRENDE RECUPERAR E) VECTOR DE A CÉLULA DE VID O DEL MEDIO DONDE SE CULTIVA LA CÉLULA DE VID.
Applications Claiming Priority (2)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
US6330508P | 2008-01-31 | 2008-01-31 | |
US8350408P | 2008-07-24 | 2008-07-24 |
Publications (1)
Publication Number | Publication Date |
---|---|
CL2009000193A1 true CL2009000193A1 (es) | 2009-08-21 |
Family
ID=40952630
Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
CL2009000193A CL2009000193A1 (es) | 2008-01-31 | 2009-01-29 | Vector para la transferencia de genes capaz de reproducirse en plantas que contienen genes del virus lr-2, celula vegetal que lo comprende, metodo de produccion de dicha construccion molecular, y procedimiento para expresar un gen heterologo en una celula vegetal. |
Country Status (9)
Country | Link |
---|---|
US (2) | US8415147B2 (es) |
EP (1) | EP2240580B1 (es) |
CN (1) | CN101978051A (es) |
AR (1) | AR070326A1 (es) |
AU (1) | AU2009210508B2 (es) |
CL (1) | CL2009000193A1 (es) |
ES (1) | ES2478041T3 (es) |
WO (1) | WO2009099877A2 (es) |
ZA (1) | ZA201005866B (es) |
Families Citing this family (11)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
US8389804B2 (en) | 2010-04-08 | 2013-03-05 | University Of Florida Research Foundation, Inc. | Viral based transient-expression vector system that allows multiple applications |
ES2477141B2 (es) * | 2010-04-08 | 2015-02-23 | University Of Florida Research Foundation, Inc. | Sistema basado en un vector viral de expresión transitoria que permite múltiples aplicaciones |
BR112013008377A2 (pt) * | 2010-10-08 | 2020-10-06 | Fraunhofer Usa Inc. | molécula de ácido nucléico isolada, para produzir dois ou mais polipeptídeos alvos em uma célula de planta, método para produzir dois ou mais polipeptídeos alvos em uma célula de planta, composição, método de tratamento de um indivíduo em necessidade de pelo menos um dentre os dois ou mais polipeptídeos alvos produzidos pelo método, método de tratamento de um indivíduo em necessidade da proteína produzida pelo método e método para induzir uma resposta imunológica protetora contra um patógeno em um indivíduo |
CN105939599B (zh) * | 2011-09-21 | 2019-10-18 | 佛罗里达大学研究基金会有限公司 | 使用柑橘衰退病毒载体进行外源基因表达 |
US10017747B2 (en) | 2011-09-21 | 2018-07-10 | University Of Florida Research Foundation, Inc. | Citrus tristeza virus based vectors for foreign gene/s expression |
CN103421839B (zh) * | 2013-07-24 | 2015-04-29 | 河南农业大学 | 瓜类褪绿黄化病毒侵染性载体的构建方法 |
CN105063038B (zh) * | 2015-09-01 | 2017-12-26 | 中华人民共和国伊犁出入境检验检疫局 | 一种检测葡萄卷叶伴随病毒3号的rpa引物及试剂盒 |
CN109777792B (zh) * | 2018-09-29 | 2020-12-11 | 中国科学院动物研究所 | 一种rna解旋酶3及其编码基因和应用 |
CN110144338A (zh) * | 2019-05-29 | 2019-08-20 | 通用生物***(安徽)有限公司 | 一种以rna为模板的rna聚合酶制备方法及该聚合酶的应用 |
MX2023008604A (es) * | 2021-01-21 | 2023-10-10 | Yissum Res Dev Co Of Hebrew Univ Jerusalem Ltd | Nanopartículas de arni y métodos de uso de las mismas en agricultura. |
CN117305267B (zh) * | 2023-08-09 | 2024-05-28 | 中国农业大学 | 葡萄类受体蛋白激酶herk1的新用途 |
Family Cites Families (5)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
US6093544A (en) | 1997-05-20 | 2000-07-25 | Cornell Research Foundation, Inc. | Rupestris stem pitting associated virus nucleic acids, proteins, and their uses |
ZA984232B (en) * | 1997-05-20 | 1998-12-01 | Cornell Res Foundation Inc | Grapevine leafroll virus type 2 proteins coding sequences and methods |
US20020104126A1 (en) | 2000-10-18 | 2002-08-01 | Pogue Gregory P. | Production of bovine lysozyme by plant viral vectors |
DE10109354A1 (de) | 2001-02-27 | 2002-09-05 | Icon Genetics Ag | Rekombinante virale Schaltersysteme |
US20060228800A1 (en) | 2003-05-15 | 2006-10-12 | Shi-Lung Lin | Novel Transgenic Methods Using intronic RNA |
-
2009
- 2009-01-29 AU AU2009210508A patent/AU2009210508B2/en not_active Ceased
- 2009-01-29 AR ARP090100293A patent/AR070326A1/es unknown
- 2009-01-29 CL CL2009000193A patent/CL2009000193A1/es unknown
- 2009-01-29 CN CN2009801107817A patent/CN101978051A/zh active Pending
- 2009-01-29 US US12/865,708 patent/US8415147B2/en active Active
- 2009-01-29 EP EP09709123.5A patent/EP2240580B1/en not_active Not-in-force
- 2009-01-29 WO PCT/US2009/032380 patent/WO2009099877A2/en active Application Filing
- 2009-01-29 ES ES09709123.5T patent/ES2478041T3/es active Active
-
2010
- 2010-08-17 ZA ZA2010/05866A patent/ZA201005866B/en unknown
-
2013
- 2013-01-29 US US13/752,605 patent/US20130133109A1/en not_active Abandoned
Also Published As
Publication number | Publication date |
---|---|
US8415147B2 (en) | 2013-04-09 |
ES2478041T3 (es) | 2014-07-18 |
EP2240580B1 (en) | 2014-04-30 |
ZA201005866B (en) | 2011-11-30 |
AU2009210508B2 (en) | 2015-04-09 |
WO2009099877A3 (en) | 2010-01-07 |
AU2009210508A1 (en) | 2009-08-13 |
WO2009099877A2 (en) | 2009-08-13 |
CN101978051A (zh) | 2011-02-16 |
EP2240580A4 (en) | 2011-09-07 |
US20130133109A1 (en) | 2013-05-23 |
US20100333230A1 (en) | 2010-12-30 |
EP2240580A2 (en) | 2010-10-20 |
AR070326A1 (es) | 2010-03-31 |
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