CA3188282A1 - Reproduction rapide de plantes cultivees transgeniques par edition genomique - Google Patents
Reproduction rapide de plantes cultivees transgeniques par edition genomiqueInfo
- Publication number
- CA3188282A1 CA3188282A1 CA3188282A CA3188282A CA3188282A1 CA 3188282 A1 CA3188282 A1 CA 3188282A1 CA 3188282 A CA3188282 A CA 3188282A CA 3188282 A CA3188282 A CA 3188282A CA 3188282 A1 CA3188282 A1 CA 3188282A1
- Authority
- CA
- Canada
- Prior art keywords
- transgenic locus
- transgenic
- dna
- plant
- approved
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Pending
Links
- 230000009261 transgenic effect Effects 0.000 title claims abstract description 829
- 244000038559 crop plants Species 0.000 title claims description 138
- 238000010362 genome editing Methods 0.000 title claims description 79
- 238000009395 breeding Methods 0.000 title description 11
- 230000001488 breeding effect Effects 0.000 title description 11
- 238000000034 method Methods 0.000 claims abstract description 190
- 241000196324 Embryophyta Species 0.000 claims description 470
- 108020004414 DNA Proteins 0.000 claims description 430
- 239000003550 marker Substances 0.000 claims description 143
- 108090000623 proteins and genes Proteins 0.000 claims description 133
- 102000040430 polynucleotide Human genes 0.000 claims description 132
- 108091033319 polynucleotide Proteins 0.000 claims description 132
- 239000002157 polynucleotide Substances 0.000 claims description 132
- 240000008042 Zea mays Species 0.000 claims description 96
- 239000002773 nucleotide Substances 0.000 claims description 94
- 125000003729 nucleotide group Chemical group 0.000 claims description 94
- 108700019146 Transgenes Proteins 0.000 claims description 90
- 230000004048 modification Effects 0.000 claims description 83
- 238000012986 modification Methods 0.000 claims description 83
- 238000012217 deletion Methods 0.000 claims description 64
- 230000037430 deletion Effects 0.000 claims description 64
- 108020005004 Guide RNA Proteins 0.000 claims description 61
- 230000002068 genetic effect Effects 0.000 claims description 60
- 150000007523 nucleic acids Chemical class 0.000 claims description 60
- 102000039446 nucleic acids Human genes 0.000 claims description 52
- 108020004707 nucleic acids Proteins 0.000 claims description 52
- 108091032973 (ribonucleotides)n+m Proteins 0.000 claims description 47
- 239000012472 biological sample Substances 0.000 claims description 42
- 108010008532 Deoxyribonuclease I Proteins 0.000 claims description 38
- 102000007260 Deoxyribonuclease I Human genes 0.000 claims description 38
- 108091040857 miR-604 stem-loop Proteins 0.000 claims description 34
- 108091088140 miR162 stem-loop Proteins 0.000 claims description 34
- 230000008859 change Effects 0.000 claims description 33
- 239000013611 chromosomal DNA Substances 0.000 claims description 28
- 235000002017 Zea mays subsp mays Nutrition 0.000 claims description 27
- 238000001514 detection method Methods 0.000 claims description 26
- 235000016383 Zea mays subsp huehuetenangensis Nutrition 0.000 claims description 25
- 235000009973 maize Nutrition 0.000 claims description 25
- 239000012634 fragment Substances 0.000 claims description 22
- 206010021929 Infertility male Diseases 0.000 claims description 20
- 208000007466 Male Infertility Diseases 0.000 claims description 20
- 238000004519 manufacturing process Methods 0.000 claims description 20
- 230000000306 recurrent effect Effects 0.000 claims description 19
- 230000001419 dependent effect Effects 0.000 claims description 17
- 238000003556 assay Methods 0.000 claims description 16
- 230000001939 inductive effect Effects 0.000 claims description 16
- 239000003795 chemical substances by application Substances 0.000 claims description 12
- 108700001094 Plant Genes Proteins 0.000 claims description 10
- 238000003306 harvesting Methods 0.000 claims description 10
- 230000035772 mutation Effects 0.000 claims description 8
- 239000000126 substance Substances 0.000 claims description 8
- 230000001086 cytosolic effect Effects 0.000 claims description 5
- 238000009331 sowing Methods 0.000 claims description 4
- 230000009418 agronomic effect Effects 0.000 claims description 2
- 238000003976 plant breeding Methods 0.000 abstract 1
- 210000004027 cell Anatomy 0.000 description 127
- 230000014509 gene expression Effects 0.000 description 57
- 102000053602 DNA Human genes 0.000 description 50
- 108091033409 CRISPR Proteins 0.000 description 33
- 239000004009 herbicide Substances 0.000 description 31
- 108010082527 phosphinothricin N-acetyltransferase Proteins 0.000 description 30
- 244000068988 Glycine max Species 0.000 description 29
- 238000003780 insertion Methods 0.000 description 29
- 230000037431 insertion Effects 0.000 description 29
- 240000000385 Brassica napus var. napus Species 0.000 description 26
- 102000004533 Endonucleases Human genes 0.000 description 25
- 108010042407 Endonucleases Proteins 0.000 description 25
- 230000002363 herbicidal effect Effects 0.000 description 25
- 230000005782 double-strand break Effects 0.000 description 24
- 101710163270 Nuclease Proteins 0.000 description 21
- HCHKCACWOHOZIP-UHFFFAOYSA-N Zinc Chemical compound [Zn] HCHKCACWOHOZIP-UHFFFAOYSA-N 0.000 description 21
- 238000003776 cleavage reaction Methods 0.000 description 21
- 230000007017 scission Effects 0.000 description 21
- 210000001519 tissue Anatomy 0.000 description 21
- 229910052725 zinc Inorganic materials 0.000 description 21
- 239000011701 zinc Substances 0.000 description 21
- 235000018102 proteins Nutrition 0.000 description 20
- 102000004169 proteins and genes Human genes 0.000 description 20
- 108091028043 Nucleic acid sequence Proteins 0.000 description 19
- 239000000203 mixture Substances 0.000 description 19
- 108091026890 Coding region Proteins 0.000 description 18
- 210000001938 protoplast Anatomy 0.000 description 18
- 240000002024 Gossypium herbaceum Species 0.000 description 16
- 235000004341 Gossypium herbaceum Nutrition 0.000 description 16
- 238000010586 diagram Methods 0.000 description 16
- 235000010469 Glycine max Nutrition 0.000 description 15
- 241000238631 Hexapoda Species 0.000 description 15
- 230000027455 binding Effects 0.000 description 15
- 230000000670 limiting effect Effects 0.000 description 14
- 108020004682 Single-Stranded DNA Proteins 0.000 description 13
- 238000010354 CRISPR gene editing Methods 0.000 description 12
- 229920000742 Cotton Polymers 0.000 description 12
- 241000219146 Gossypium Species 0.000 description 12
- 108010017070 Zinc Finger Nucleases Proteins 0.000 description 12
- 210000002230 centromere Anatomy 0.000 description 11
- 230000008569 process Effects 0.000 description 11
- 108091035539 telomere Proteins 0.000 description 11
- 102000055501 telomere Human genes 0.000 description 11
- 210000003411 telomere Anatomy 0.000 description 11
- 108700004991 Cas12a Proteins 0.000 description 10
- 230000004568 DNA-binding Effects 0.000 description 10
- 210000000349 chromosome Anatomy 0.000 description 10
- 230000034431 double-strand break repair via homologous recombination Effects 0.000 description 10
- 235000013305 food Nutrition 0.000 description 10
- 230000001404 mediated effect Effects 0.000 description 10
- 238000006467 substitution reaction Methods 0.000 description 10
- 102000040650 (ribonucleotides)n+m Human genes 0.000 description 9
- 235000014698 Brassica juncea var multisecta Nutrition 0.000 description 9
- 235000006008 Brassica napus var napus Nutrition 0.000 description 9
- 235000006618 Brassica rapa subsp oleifera Nutrition 0.000 description 9
- 235000004977 Brassica sinapistrum Nutrition 0.000 description 9
- 239000005562 Glyphosate Substances 0.000 description 9
- 108700011259 MicroRNAs Proteins 0.000 description 9
- 108010073062 Transcription Activator-Like Effectors Proteins 0.000 description 9
- 230000000694 effects Effects 0.000 description 9
- 229940097068 glyphosate Drugs 0.000 description 9
- 239000002679 microRNA Substances 0.000 description 9
- 230000001105 regulatory effect Effects 0.000 description 9
- 230000003007 single stranded DNA break Effects 0.000 description 9
- 108010020183 3-phosphoshikimate 1-carboxyvinyltransferase Proteins 0.000 description 8
- 238000013461 design Methods 0.000 description 8
- 108090000765 processed proteins & peptides Proteins 0.000 description 8
- 206010020649 Hyperkeratosis Diseases 0.000 description 7
- 238000010459 TALEN Methods 0.000 description 7
- 108010043645 Transcription Activator-Like Effector Nucleases Proteins 0.000 description 7
- 230000005017 genetic modification Effects 0.000 description 7
- XDDAORKBJWWYJS-UHFFFAOYSA-N glyphosate Chemical compound OC(=O)CNCP(O)(O)=O XDDAORKBJWWYJS-UHFFFAOYSA-N 0.000 description 7
- 230000008488 polyadenylation Effects 0.000 description 7
- 229920001184 polypeptide Polymers 0.000 description 7
- 102000004196 processed proteins & peptides Human genes 0.000 description 7
- 241000894007 species Species 0.000 description 7
- 108091027544 Subgenomic mRNA Proteins 0.000 description 6
- 230000003115 biocidal effect Effects 0.000 description 6
- 210000000170 cell membrane Anatomy 0.000 description 6
- 230000000295 complement effect Effects 0.000 description 6
- 210000002257 embryonic structure Anatomy 0.000 description 6
- 238000009396 hybridization Methods 0.000 description 6
- 239000000047 product Substances 0.000 description 6
- 230000001172 regenerating effect Effects 0.000 description 6
- 238000011282 treatment Methods 0.000 description 6
- 108020005345 3' Untranslated Regions Proteins 0.000 description 5
- 230000007018 DNA scission Effects 0.000 description 5
- 229920002472 Starch Polymers 0.000 description 5
- 230000036579 abiotic stress Effects 0.000 description 5
- GFFGJBXGBJISGV-UHFFFAOYSA-N adenyl group Chemical group N1=CN=C2N=CNC2=C1N GFFGJBXGBJISGV-UHFFFAOYSA-N 0.000 description 5
- 125000003275 alpha amino acid group Chemical group 0.000 description 5
- 150000001413 amino acids Chemical class 0.000 description 5
- 239000002551 biofuel Substances 0.000 description 5
- 210000002421 cell wall Anatomy 0.000 description 5
- 230000004049 epigenetic modification Effects 0.000 description 5
- 210000003527 eukaryotic cell Anatomy 0.000 description 5
- 230000002779 inactivation Effects 0.000 description 5
- 235000012054 meals Nutrition 0.000 description 5
- 230000006780 non-homologous end joining Effects 0.000 description 5
- 235000019698 starch Nutrition 0.000 description 5
- IAJOBQBIJHVGMQ-UHFFFAOYSA-N 2-amino-4-[hydroxy(methyl)phosphoryl]butanoic acid Chemical compound CP(O)(=O)CCC(N)C(O)=O IAJOBQBIJHVGMQ-UHFFFAOYSA-N 0.000 description 4
- WQZGKKKJIJFFOK-QTVWNMPRSA-N D-mannopyranose Chemical compound OC[C@H]1OC(O)[C@@H](O)[C@@H](O)[C@@H]1O WQZGKKKJIJFFOK-QTVWNMPRSA-N 0.000 description 4
- 238000010442 DNA editing Methods 0.000 description 4
- 102000052510 DNA-Binding Proteins Human genes 0.000 description 4
- 239000005561 Glufosinate Substances 0.000 description 4
- 240000007594 Oryza sativa Species 0.000 description 4
- 235000007164 Oryza sativa Nutrition 0.000 description 4
- 101710185494 Zinc finger protein Proteins 0.000 description 4
- 102100023597 Zinc finger protein 816 Human genes 0.000 description 4
- 239000011230 binding agent Substances 0.000 description 4
- OPTASPLRGRRNAP-UHFFFAOYSA-N cytosine Chemical compound NC=1C=CNC(=O)N=1 OPTASPLRGRRNAP-UHFFFAOYSA-N 0.000 description 4
- 230000010354 integration Effects 0.000 description 4
- 239000003147 molecular marker Substances 0.000 description 4
- 239000003921 oil Substances 0.000 description 4
- 230000003252 repetitive effect Effects 0.000 description 4
- 239000008107 starch Substances 0.000 description 4
- 235000000346 sugar Nutrition 0.000 description 4
- 230000008685 targeting Effects 0.000 description 4
- 239000013598 vector Substances 0.000 description 4
- 230000017260 vegetative to reproductive phase transition of meristem Effects 0.000 description 4
- 108020003589 5' Untranslated Regions Proteins 0.000 description 3
- 229930024421 Adenine Natural products 0.000 description 3
- 108010031325 Cytidine deaminase Proteins 0.000 description 3
- 101710096438 DNA-binding protein Proteins 0.000 description 3
- 239000005504 Dicamba Substances 0.000 description 3
- LFQSCWFLJHTTHZ-UHFFFAOYSA-N Ethanol Chemical compound CCO LFQSCWFLJHTTHZ-UHFFFAOYSA-N 0.000 description 3
- 241000244206 Nematoda Species 0.000 description 3
- 102000014450 RNA Polymerase III Human genes 0.000 description 3
- 108010078067 RNA Polymerase III Proteins 0.000 description 3
- 108010091086 Recombinases Proteins 0.000 description 3
- 102000018120 Recombinases Human genes 0.000 description 3
- -1 TALENS Proteins 0.000 description 3
- 108091028113 Trans-activating crRNA Proteins 0.000 description 3
- 108091023040 Transcription factor Proteins 0.000 description 3
- 102000040945 Transcription factor Human genes 0.000 description 3
- 241000607479 Yersinia pestis Species 0.000 description 3
- 238000005299 abrasion Methods 0.000 description 3
- 229960000643 adenine Drugs 0.000 description 3
- 230000003321 amplification Effects 0.000 description 3
- 238000013459 approach Methods 0.000 description 3
- 230000001580 bacterial effect Effects 0.000 description 3
- 230000004790 biotic stress Effects 0.000 description 3
- 230000002759 chromosomal effect Effects 0.000 description 3
- 150000001875 compounds Chemical class 0.000 description 3
- 238000005520 cutting process Methods 0.000 description 3
- 230000003111 delayed effect Effects 0.000 description 3
- IWEDIXLBFLAXBO-UHFFFAOYSA-N dicamba Chemical compound COC1=C(Cl)C=CC(Cl)=C1C(O)=O IWEDIXLBFLAXBO-UHFFFAOYSA-N 0.000 description 3
- 230000002255 enzymatic effect Effects 0.000 description 3
- 230000004720 fertilization Effects 0.000 description 3
- 230000012010 growth Effects 0.000 description 3
- 230000006872 improvement Effects 0.000 description 3
- 238000002955 isolation Methods 0.000 description 3
- 108010058731 nopaline synthase Proteins 0.000 description 3
- 238000003199 nucleic acid amplification method Methods 0.000 description 3
- 230000006798 recombination Effects 0.000 description 3
- 238000005215 recombination Methods 0.000 description 3
- 210000005132 reproductive cell Anatomy 0.000 description 3
- 238000007894 restriction fragment length polymorphism technique Methods 0.000 description 3
- 235000009566 rice Nutrition 0.000 description 3
- 230000009870 specific binding Effects 0.000 description 3
- 150000008163 sugars Chemical class 0.000 description 3
- YBJHBAHKTGYVGT-ZKWXMUAHSA-N (+)-Biotin Chemical compound N1C(=O)N[C@@H]2[C@H](CCCCC(=O)O)SC[C@@H]21 YBJHBAHKTGYVGT-ZKWXMUAHSA-N 0.000 description 2
- WRIDQFICGBMAFQ-UHFFFAOYSA-N (E)-8-Octadecenoic acid Natural products CCCCCCCCCC=CCCCCCCC(O)=O WRIDQFICGBMAFQ-UHFFFAOYSA-N 0.000 description 2
- IAKHMKGGTNLKSZ-INIZCTEOSA-N (S)-colchicine Chemical compound C1([C@@H](NC(C)=O)CC2)=CC(=O)C(OC)=CC=C1C1=C2C=C(OC)C(OC)=C1OC IAKHMKGGTNLKSZ-INIZCTEOSA-N 0.000 description 2
- LQJBNNIYVWPHFW-UHFFFAOYSA-N 20:1omega9c fatty acid Natural products CCCCCCCCCCC=CCCCCCCCC(O)=O LQJBNNIYVWPHFW-UHFFFAOYSA-N 0.000 description 2
- QSBYPNXLFMSGKH-UHFFFAOYSA-N 9-Heptadecensaeure Natural products CCCCCCCC=CCCCCCCCC(O)=O QSBYPNXLFMSGKH-UHFFFAOYSA-N 0.000 description 2
- 108010052875 Adenine deaminase Proteins 0.000 description 2
- 241000589155 Agrobacterium tumefaciens Species 0.000 description 2
- 108091023037 Aptamer Proteins 0.000 description 2
- 241000219195 Arabidopsis thaliana Species 0.000 description 2
- 101100507772 Arabidopsis thaliana HTR12 gene Proteins 0.000 description 2
- IJGRMHOSHXDMSA-UHFFFAOYSA-N Atomic nitrogen Chemical compound N#N IJGRMHOSHXDMSA-UHFFFAOYSA-N 0.000 description 2
- 108010016529 Bacillus amyloliquefaciens ribonuclease Proteins 0.000 description 2
- 241000894006 Bacteria Species 0.000 description 2
- 108091032955 Bacterial small RNA Proteins 0.000 description 2
- 239000002028 Biomass Substances 0.000 description 2
- 229910001369 Brass Inorganic materials 0.000 description 2
- OKTJSMMVPCPJKN-UHFFFAOYSA-N Carbon Chemical compound [C] OKTJSMMVPCPJKN-UHFFFAOYSA-N 0.000 description 2
- 102100026846 Cytidine deaminase Human genes 0.000 description 2
- 108010066133 D-octopine dehydrogenase Proteins 0.000 description 2
- 108091029865 Exogenous DNA Proteins 0.000 description 2
- 241000701484 Figwort mosaic virus Species 0.000 description 2
- 108010017464 Fructose-Bisphosphatase Proteins 0.000 description 2
- 229940123611 Genome editing Drugs 0.000 description 2
- 108091092195 Intron Proteins 0.000 description 2
- 108010025815 Kanamycin Kinase Proteins 0.000 description 2
- 235000007688 Lycopersicon esculentum Nutrition 0.000 description 2
- 108091022912 Mannose-6-Phosphate Isomerase Proteins 0.000 description 2
- 102000048193 Mannose-6-phosphate isomerases Human genes 0.000 description 2
- 241001465754 Metazoa Species 0.000 description 2
- GQPLMRYTRLFLPF-UHFFFAOYSA-N Nitrous Oxide Chemical compound [O-][N+]#N GQPLMRYTRLFLPF-UHFFFAOYSA-N 0.000 description 2
- 239000005642 Oleic acid Substances 0.000 description 2
- ZQPPMHVWECSIRJ-UHFFFAOYSA-N Oleic acid Natural products CCCCCCCCC=CCCCCCCCC(O)=O ZQPPMHVWECSIRJ-UHFFFAOYSA-N 0.000 description 2
- 108700026244 Open Reading Frames Proteins 0.000 description 2
- 102000004316 Oxidoreductases Human genes 0.000 description 2
- 108090000854 Oxidoreductases Proteins 0.000 description 2
- 102000003867 Phospholipid Transfer Proteins Human genes 0.000 description 2
- 108090000216 Phospholipid Transfer Proteins Proteins 0.000 description 2
- 208000020584 Polyploidy Diseases 0.000 description 2
- 108091027981 Response element Proteins 0.000 description 2
- 108010052160 Site-specific recombinase Proteins 0.000 description 2
- 240000003768 Solanum lycopersicum Species 0.000 description 2
- 241000194020 Streptococcus thermophilus Species 0.000 description 2
- 108091036066 Three prime untranslated region Proteins 0.000 description 2
- 102000004357 Transferases Human genes 0.000 description 2
- 108090000992 Transferases Proteins 0.000 description 2
- 241000209140 Triticum Species 0.000 description 2
- 235000021307 Triticum Nutrition 0.000 description 2
- DRTQHJPVMGBUCF-XVFCMESISA-N Uridine Chemical compound O[C@@H]1[C@H](O)[C@@H](CO)O[C@H]1N1C(=O)NC(=O)C=C1 DRTQHJPVMGBUCF-XVFCMESISA-N 0.000 description 2
- 235000005824 Zea mays ssp. parviglumis Nutrition 0.000 description 2
- 230000004075 alteration Effects 0.000 description 2
- 229940126575 aminoglycoside Drugs 0.000 description 2
- 239000010951 brass Substances 0.000 description 2
- 235000014633 carbohydrates Nutrition 0.000 description 2
- 150000001720 carbohydrates Chemical class 0.000 description 2
- 229910052799 carbon Inorganic materials 0.000 description 2
- 230000022131 cell cycle Effects 0.000 description 2
- 210000003763 chloroplast Anatomy 0.000 description 2
- 238000004891 communication Methods 0.000 description 2
- 235000005822 corn Nutrition 0.000 description 2
- 229940104302 cytosine Drugs 0.000 description 2
- 230000008641 drought stress Effects 0.000 description 2
- 238000009510 drug design Methods 0.000 description 2
- 239000000839 emulsion Substances 0.000 description 2
- 238000005516 engineering process Methods 0.000 description 2
- 230000001747 exhibiting effect Effects 0.000 description 2
- 230000004927 fusion Effects 0.000 description 2
- 238000012239 gene modification Methods 0.000 description 2
- 235000013617 genetically modified food Nutrition 0.000 description 2
- 210000004602 germ cell Anatomy 0.000 description 2
- 239000005556 hormone Substances 0.000 description 2
- 229940088597 hormone Drugs 0.000 description 2
- 108010002685 hygromycin-B kinase Proteins 0.000 description 2
- 238000000338 in vitro Methods 0.000 description 2
- 238000010348 incorporation Methods 0.000 description 2
- 239000000411 inducer Substances 0.000 description 2
- QXJSBBXBKPUZAA-UHFFFAOYSA-N isooleic acid Natural products CCCCCCCC=CCCCCCCCCC(O)=O QXJSBBXBKPUZAA-UHFFFAOYSA-N 0.000 description 2
- 238000005304 joining Methods 0.000 description 2
- 239000003446 ligand Substances 0.000 description 2
- 150000002632 lipids Chemical class 0.000 description 2
- 239000002502 liposome Substances 0.000 description 2
- 239000007788 liquid Substances 0.000 description 2
- 239000000463 material Substances 0.000 description 2
- 238000000520 microinjection Methods 0.000 description 2
- 230000000921 morphogenic effect Effects 0.000 description 2
- 239000002105 nanoparticle Substances 0.000 description 2
- 238000007481 next generation sequencing Methods 0.000 description 2
- 235000019198 oils Nutrition 0.000 description 2
- ZQPPMHVWECSIRJ-KTKRTIGZSA-N oleic acid Chemical compound CCCCCCCC\C=C/CCCCCCCC(O)=O ZQPPMHVWECSIRJ-KTKRTIGZSA-N 0.000 description 2
- 210000000056 organ Anatomy 0.000 description 2
- 239000013612 plasmid Substances 0.000 description 2
- 229920002401 polyacrylamide Polymers 0.000 description 2
- 238000003752 polymerase chain reaction Methods 0.000 description 2
- 239000012264 purified product Substances 0.000 description 2
- 230000008707 rearrangement Effects 0.000 description 2
- 230000009467 reduction Effects 0.000 description 2
- 230000002829 reductive effect Effects 0.000 description 2
- 230000008929 regeneration Effects 0.000 description 2
- 238000011069 regeneration method Methods 0.000 description 2
- 108091008146 restriction endonucleases Proteins 0.000 description 2
- 150000003839 salts Chemical class 0.000 description 2
- 239000000523 sample Substances 0.000 description 2
- 230000010153 self-pollination Effects 0.000 description 2
- 238000013518 transcription Methods 0.000 description 2
- 230000035897 transcription Effects 0.000 description 2
- 239000005631 2,4-Dichlorophenoxyacetic acid Substances 0.000 description 1
- MWMOPIVLTLEUJO-UHFFFAOYSA-N 2-oxopropanoic acid;phosphoric acid Chemical compound OP(O)(O)=O.CC(=O)C(O)=O MWMOPIVLTLEUJO-UHFFFAOYSA-N 0.000 description 1
- FWMNVWWHGCHHJJ-SKKKGAJSSA-N 4-amino-1-[(2r)-6-amino-2-[[(2r)-2-[[(2r)-2-[[(2r)-2-amino-3-phenylpropanoyl]amino]-3-phenylpropanoyl]amino]-4-methylpentanoyl]amino]hexanoyl]piperidine-4-carboxylic acid Chemical compound C([C@H](C(=O)N[C@H](CC(C)C)C(=O)N[C@H](CCCCN)C(=O)N1CCC(N)(CC1)C(O)=O)NC(=O)[C@H](N)CC=1C=CC=CC=1)C1=CC=CC=C1 FWMNVWWHGCHHJJ-SKKKGAJSSA-N 0.000 description 1
- 102000007469 Actins Human genes 0.000 description 1
- 108010085238 Actins Proteins 0.000 description 1
- 101150092509 Actn gene Proteins 0.000 description 1
- 241000589158 Agrobacterium Species 0.000 description 1
- 241000589159 Agrobacterium sp. Species 0.000 description 1
- 108700028369 Alleles Proteins 0.000 description 1
- 229920000945 Amylopectin Polymers 0.000 description 1
- 102000008682 Argonaute Proteins Human genes 0.000 description 1
- 108010088141 Argonaute Proteins Proteins 0.000 description 1
- 229930192334 Auxin Natural products 0.000 description 1
- 208000035143 Bacterial infection Diseases 0.000 description 1
- 241000589171 Bradyrhizobium sp. Species 0.000 description 1
- 101100004199 Brassica napus BBM2 gene Proteins 0.000 description 1
- 101710172824 CRISPR-associated endonuclease Cas9 Proteins 0.000 description 1
- 101100442689 Caenorhabditis elegans hdl-1 gene Proteins 0.000 description 1
- 102000014914 Carrier Proteins Human genes 0.000 description 1
- 108090000994 Catalytic RNA Proteins 0.000 description 1
- 102000053642 Catalytic RNA Human genes 0.000 description 1
- 108050006476 Chlorophyll A-B binding proteins Proteins 0.000 description 1
- VPAXJOUATWLOPR-UHFFFAOYSA-N Conferone Chemical compound C1=CC(=O)OC2=CC(OCC3C4(C)CCC(=O)C(C)(C)C4CC=C3C)=CC=C21 VPAXJOUATWLOPR-UHFFFAOYSA-N 0.000 description 1
- 208000034656 Contusions Diseases 0.000 description 1
- 102000005381 Cytidine Deaminase Human genes 0.000 description 1
- 102220605874 Cytosolic arginine sensor for mTORC1 subunit 2_D10A_mutation Human genes 0.000 description 1
- 102000012410 DNA Ligases Human genes 0.000 description 1
- 108010061982 DNA Ligases Proteins 0.000 description 1
- 101710150423 DNA nickase Proteins 0.000 description 1
- 230000033616 DNA repair Effects 0.000 description 1
- 108700020911 DNA-Binding Proteins Proteins 0.000 description 1
- 208000035240 Disease Resistance Diseases 0.000 description 1
- 241001057636 Dracaena deremensis Species 0.000 description 1
- 101100109120 Drosophila melanogaster Alp4 gene Proteins 0.000 description 1
- 101100480904 Drosophila melanogaster tctn gene Proteins 0.000 description 1
- 102000002322 Egg Proteins Human genes 0.000 description 1
- 108010000912 Egg Proteins Proteins 0.000 description 1
- 241000702189 Escherichia virus Mu Species 0.000 description 1
- VGGSQFUCUMXWEO-UHFFFAOYSA-N Ethene Chemical compound C=C VGGSQFUCUMXWEO-UHFFFAOYSA-N 0.000 description 1
- 239000005977 Ethylene Substances 0.000 description 1
- 241000206602 Eukaryota Species 0.000 description 1
- MBMLMWLHJBBADN-UHFFFAOYSA-N Ferrous sulfide Chemical compound [Fe]=S MBMLMWLHJBBADN-UHFFFAOYSA-N 0.000 description 1
- 102000027487 Fructose-Bisphosphatase Human genes 0.000 description 1
- 102000001390 Fructose-Bisphosphate Aldolase Human genes 0.000 description 1
- 108010068561 Fructose-Bisphosphate Aldolase Proteins 0.000 description 1
- 241000702463 Geminiviridae Species 0.000 description 1
- 102000053187 Glucuronidase Human genes 0.000 description 1
- 108010060309 Glucuronidase Proteins 0.000 description 1
- 108010068370 Glutens Proteins 0.000 description 1
- 102100024501 Histone H3-like centromeric protein A Human genes 0.000 description 1
- 101000981071 Homo sapiens Histone H3-like centromeric protein A Proteins 0.000 description 1
- 101000829367 Homo sapiens Src substrate cortactin Proteins 0.000 description 1
- 240000005979 Hordeum vulgare Species 0.000 description 1
- 235000007340 Hordeum vulgare Nutrition 0.000 description 1
- IMQLKJBTEOYOSI-GPIVLXJGSA-N Inositol-hexakisphosphate Chemical compound OP(O)(=O)O[C@H]1[C@H](OP(O)(O)=O)[C@@H](OP(O)(O)=O)[C@H](OP(O)(O)=O)[C@H](OP(O)(O)=O)[C@@H]1OP(O)(O)=O IMQLKJBTEOYOSI-GPIVLXJGSA-N 0.000 description 1
- 102000005298 Iron-Sulfur Proteins Human genes 0.000 description 1
- 108010081409 Iron-Sulfur Proteins Proteins 0.000 description 1
- 108010044467 Isoenzymes Proteins 0.000 description 1
- 239000005571 Isoxaflutole Substances 0.000 description 1
- 101100288095 Klebsiella pneumoniae neo gene Proteins 0.000 description 1
- 102000003855 L-lactate dehydrogenase Human genes 0.000 description 1
- 108700023483 L-lactate dehydrogenases Proteins 0.000 description 1
- 108091026898 Leader sequence (mRNA) Proteins 0.000 description 1
- 241000288904 Lemur Species 0.000 description 1
- 241000209510 Liliopsida Species 0.000 description 1
- 239000000232 Lipid Bilayer Substances 0.000 description 1
- 241000061177 Mesorhizobium sp. Species 0.000 description 1
- 239000005578 Mesotrione Substances 0.000 description 1
- 108091092878 Microsatellite Proteins 0.000 description 1
- 101001094044 Mus musculus Solute carrier family 26 member 6 Proteins 0.000 description 1
- 101100406338 Mycoplasma capricolum subsp. capricolum (strain California kid / ATCC 27343 / NCTC 10154) pdhC gene Proteins 0.000 description 1
- 208000031888 Mycoses Diseases 0.000 description 1
- 101150094405 NAC23 gene Proteins 0.000 description 1
- 241000588650 Neisseria meningitidis Species 0.000 description 1
- 108020004485 Nonsense Codon Proteins 0.000 description 1
- 108020005497 Nuclear hormone receptor Proteins 0.000 description 1
- 102000007399 Nuclear hormone receptor Human genes 0.000 description 1
- 108020004711 Nucleic Acid Probes Proteins 0.000 description 1
- 108091034117 Oligonucleotide Proteins 0.000 description 1
- 101100049728 Oryza sativa subsp. japonica WOX9 gene Proteins 0.000 description 1
- 239000005587 Oryzalin Substances 0.000 description 1
- 238000012408 PCR amplification Methods 0.000 description 1
- 108010081996 Photosystem I Protein Complex Proteins 0.000 description 1
- 241000056147 Phyllobacterium sp. Species 0.000 description 1
- 241000589187 Rhizobium sp. Species 0.000 description 1
- 108020004422 Riboswitch Proteins 0.000 description 1
- 108010003581 Ribulose-bisphosphate carboxylase Proteins 0.000 description 1
- 241000225041 Roestes Species 0.000 description 1
- 101100462087 Saccharomyces cerevisiae (strain ATCC 204508 / S288c) LAT1 gene Proteins 0.000 description 1
- 101710142587 Short-chain dehydrogenase/reductase Proteins 0.000 description 1
- 108091061750 Signal recognition particle RNA Proteins 0.000 description 1
- 241000144249 Sinorhizobium sp. Species 0.000 description 1
- 108020004459 Small interfering RNA Proteins 0.000 description 1
- 240000006394 Sorghum bicolor Species 0.000 description 1
- 235000011684 Sorghum saccharatum Nutrition 0.000 description 1
- 102100023719 Src substrate cortactin Human genes 0.000 description 1
- 101100166144 Staphylococcus aureus cas9 gene Proteins 0.000 description 1
- 241000193996 Streptococcus pyogenes Species 0.000 description 1
- 108020004566 Transfer RNA Proteins 0.000 description 1
- 101800005109 Triakontatetraneuropeptide Proteins 0.000 description 1
- 244000098338 Triticum aestivum Species 0.000 description 1
- 101000987816 Triticum aestivum 16.9 kDa class I heat shock protein 1 Proteins 0.000 description 1
- 108090000704 Tubulin Proteins 0.000 description 1
- IBEDDHUHZBDXGB-OEJISELMSA-N Tubulysin A Chemical compound N([C@@H]([C@@H](C)CC)C(=O)N(COC(=O)CC(C)C)[C@H](C[C@@H](OC(C)=O)C=1SC=C(N=1)C(=O)N[C@H](C[C@H](C)C(O)=O)CC=1C=CC(O)=CC=1)C(C)C)C(=O)[C@H]1CCCCN1C IBEDDHUHZBDXGB-OEJISELMSA-N 0.000 description 1
- 108091026822 U6 spliceosomal RNA Proteins 0.000 description 1
- 108090000848 Ubiquitin Proteins 0.000 description 1
- 102000044159 Ubiquitin Human genes 0.000 description 1
- 108091023045 Untranslated Region Proteins 0.000 description 1
- 101150065399 WOX4 gene Proteins 0.000 description 1
- 101150010537 WOX5 gene Proteins 0.000 description 1
- 101150019635 WOX9 gene Proteins 0.000 description 1
- 241000589634 Xanthomonas Species 0.000 description 1
- KRPUGSACBHJZSR-UHFFFAOYSA-N [3-oxo-2-phenyl-3-(pyridin-2-ylmethylamino)propyl] acetate Chemical compound C=1C=CC=CC=1C(COC(=O)C)C(=O)NCC1=CC=CC=N1 KRPUGSACBHJZSR-UHFFFAOYSA-N 0.000 description 1
- 239000002253 acid Substances 0.000 description 1
- 210000001789 adipocyte Anatomy 0.000 description 1
- 239000000443 aerosol Substances 0.000 description 1
- 239000003242 anti bacterial agent Substances 0.000 description 1
- 230000001946 anti-microtubular Effects 0.000 description 1
- 229940044684 anti-microtubule agent Drugs 0.000 description 1
- 238000003491 array Methods 0.000 description 1
- 239000002363 auxin Substances 0.000 description 1
- 208000022362 bacterial infectious disease Diseases 0.000 description 1
- 244000052616 bacterial pathogen Species 0.000 description 1
- 230000004888 barrier function Effects 0.000 description 1
- DRTQHJPVMGBUCF-PSQAKQOGSA-N beta-L-uridine Natural products O[C@H]1[C@@H](O)[C@H](CO)O[C@@H]1N1C(=O)NC(=O)C=C1 DRTQHJPVMGBUCF-PSQAKQOGSA-N 0.000 description 1
- 108091008324 binding proteins Proteins 0.000 description 1
- 230000005540 biological transmission Effects 0.000 description 1
- 230000015572 biosynthetic process Effects 0.000 description 1
- 229960002685 biotin Drugs 0.000 description 1
- 235000020958 biotin Nutrition 0.000 description 1
- 239000011616 biotin Substances 0.000 description 1
- 239000006227 byproduct Substances 0.000 description 1
- 239000003518 caustics Substances 0.000 description 1
- 238000004113 cell culture Methods 0.000 description 1
- 230000032823 cell division Effects 0.000 description 1
- 239000013592 cell lysate Substances 0.000 description 1
- 238000005119 centrifugation Methods 0.000 description 1
- 239000004464 cereal grain Substances 0.000 description 1
- 235000013339 cereals Nutrition 0.000 description 1
- 235000001729 chan in Nutrition 0.000 description 1
- 239000003153 chemical reaction reagent Substances 0.000 description 1
- 238000010367 cloning Methods 0.000 description 1
- 229960001338 colchicine Drugs 0.000 description 1
- 239000000084 colloidal system Substances 0.000 description 1
- JECGPMYZUFFYJW-UHFFFAOYSA-N conferone Natural products CC1=CCC2C(C)(C)C(=O)CCC2(C)C1COc3cccc4C=CC(=O)Oc34 JECGPMYZUFFYJW-UHFFFAOYSA-N 0.000 description 1
- 239000000356 contaminant Substances 0.000 description 1
- 101150049887 cspB gene Proteins 0.000 description 1
- 101150041068 cspJ gene Proteins 0.000 description 1
- 101150010904 cspLB gene Proteins 0.000 description 1
- UHDGCWIWMRVCDJ-XVFCMESISA-N cytidine Chemical compound O=C1N=C(N)C=CN1[C@H]1[C@H](O)[C@H](O)[C@@H](CO)O1 UHDGCWIWMRVCDJ-XVFCMESISA-N 0.000 description 1
- 230000006378 damage Effects 0.000 description 1
- 230000003247 decreasing effect Effects 0.000 description 1
- 229940124447 delivery agent Drugs 0.000 description 1
- 238000011161 development Methods 0.000 description 1
- 230000018109 developmental process Effects 0.000 description 1
- 238000002405 diagnostic procedure Methods 0.000 description 1
- 235000014113 dietary fatty acids Nutrition 0.000 description 1
- 239000000539 dimer Substances 0.000 description 1
- NAGJZTKCGNOGPW-UHFFFAOYSA-K dioxido-sulfanylidene-sulfido-$l^{5}-phosphane Chemical group [O-]P([O-])([S-])=S NAGJZTKCGNOGPW-UHFFFAOYSA-K 0.000 description 1
- 238000007598 dipping method Methods 0.000 description 1
- 239000006185 dispersion Substances 0.000 description 1
- 230000003828 downregulation Effects 0.000 description 1
- 230000024346 drought recovery Effects 0.000 description 1
- 239000003814 drug Substances 0.000 description 1
- 229940079593 drug Drugs 0.000 description 1
- 210000005069 ears Anatomy 0.000 description 1
- 235000013399 edible fruits Nutrition 0.000 description 1
- 238000004520 electroporation Methods 0.000 description 1
- 238000012407 engineering method Methods 0.000 description 1
- 230000007613 environmental effect Effects 0.000 description 1
- 239000012167 epicuticular wax Substances 0.000 description 1
- 241001233957 eudicotyledons Species 0.000 description 1
- 239000013604 expression vector Substances 0.000 description 1
- 229930195729 fatty acid Natural products 0.000 description 1
- 239000000194 fatty acid Substances 0.000 description 1
- 150000004665 fatty acids Chemical class 0.000 description 1
- 238000000855 fermentation Methods 0.000 description 1
- 230000004151 fermentation Effects 0.000 description 1
- 239000000796 flavoring agent Substances 0.000 description 1
- 235000019634 flavors Nutrition 0.000 description 1
- 235000013312 flour Nutrition 0.000 description 1
- 239000012530 fluid Substances 0.000 description 1
- GNBHRKFJIUUOQI-UHFFFAOYSA-N fluorescein Chemical compound O1C(=O)C2=CC=CC=C2C21C1=CC=C(O)C=C1OC1=CC(O)=CC=C21 GNBHRKFJIUUOQI-UHFFFAOYSA-N 0.000 description 1
- 102000034287 fluorescent proteins Human genes 0.000 description 1
- 108091006047 fluorescent proteins Proteins 0.000 description 1
- 230000002538 fungal effect Effects 0.000 description 1
- 244000053095 fungal pathogen Species 0.000 description 1
- 239000007789 gas Substances 0.000 description 1
- 239000000499 gel Substances 0.000 description 1
- 238000010353 genetic engineering Methods 0.000 description 1
- 231100000025 genetic toxicology Toxicity 0.000 description 1
- 244000037671 genetically modified crops Species 0.000 description 1
- 230000001738 genotoxic effect Effects 0.000 description 1
- 108010086476 glycerate kinase Proteins 0.000 description 1
- 150000003278 haem Chemical class 0.000 description 1
- 210000003783 haploid cell Anatomy 0.000 description 1
- 230000001744 histochemical effect Effects 0.000 description 1
- 239000003906 humectant Substances 0.000 description 1
- 239000010903 husk Substances 0.000 description 1
- 230000015784 hyperosmotic salinity response Effects 0.000 description 1
- 230000008676 import Effects 0.000 description 1
- SEOVTRFCIGRIMH-UHFFFAOYSA-N indole-3-acetic acid Chemical compound C1=CC=C2C(CC(=O)O)=CNC2=C1 SEOVTRFCIGRIMH-UHFFFAOYSA-N 0.000 description 1
- 230000006698 induction Effects 0.000 description 1
- 230000008595 infiltration Effects 0.000 description 1
- 238000001764 infiltration Methods 0.000 description 1
- 239000004615 ingredient Substances 0.000 description 1
- 239000003112 inhibitor Substances 0.000 description 1
- 229910052500 inorganic mineral Inorganic materials 0.000 description 1
- 238000007689 inspection Methods 0.000 description 1
- 238000007852 inverse PCR Methods 0.000 description 1
- OYIKARCXOQLFHF-UHFFFAOYSA-N isoxaflutole Chemical compound CS(=O)(=O)C1=CC(C(F)(F)F)=CC=C1C(=O)C1=C(C2CC2)ON=C1 OYIKARCXOQLFHF-UHFFFAOYSA-N 0.000 description 1
- 229940088649 isoxaflutole Drugs 0.000 description 1
- 239000012978 lignocellulosic material Substances 0.000 description 1
- 108010026228 mRNA guanylyltransferase Proteins 0.000 description 1
- 238000004949 mass spectrometry Methods 0.000 description 1
- 230000021121 meiosis Effects 0.000 description 1
- 230000000442 meristematic effect Effects 0.000 description 1
- KPUREKXXPHOJQT-UHFFFAOYSA-N mesotrione Chemical compound [O-][N+](=O)C1=CC(S(=O)(=O)C)=CC=C1C(=O)C1C(=O)CCCC1=O KPUREKXXPHOJQT-UHFFFAOYSA-N 0.000 description 1
- 108020004999 messenger RNA Proteins 0.000 description 1
- 230000002503 metabolic effect Effects 0.000 description 1
- 239000002184 metal Substances 0.000 description 1
- 229910052751 metal Inorganic materials 0.000 description 1
- YACKEPLHDIMKIO-UHFFFAOYSA-N methylphosphonic acid Chemical group CP(O)(O)=O YACKEPLHDIMKIO-UHFFFAOYSA-N 0.000 description 1
- 239000000693 micelle Substances 0.000 description 1
- 239000011707 mineral Substances 0.000 description 1
- 210000003470 mitochondria Anatomy 0.000 description 1
- 230000000394 mitotic effect Effects 0.000 description 1
- 239000000178 monomer Substances 0.000 description 1
- 230000000877 morphologic effect Effects 0.000 description 1
- 244000000175 nematode pathogen Species 0.000 description 1
- 229910052757 nitrogen Inorganic materials 0.000 description 1
- 239000001272 nitrous oxide Substances 0.000 description 1
- 210000000633 nuclear envelope Anatomy 0.000 description 1
- 108020004017 nuclear receptors Proteins 0.000 description 1
- 239000002853 nucleic acid probe Substances 0.000 description 1
- 239000002777 nucleoside Substances 0.000 description 1
- 229940127073 nucleoside analogue Drugs 0.000 description 1
- 150000003833 nucleoside derivatives Chemical class 0.000 description 1
- 210000004940 nucleus Anatomy 0.000 description 1
- 235000015097 nutrients Nutrition 0.000 description 1
- 230000009437 off-target effect Effects 0.000 description 1
- 210000003463 organelle Anatomy 0.000 description 1
- UNAHYJYOSSSJHH-UHFFFAOYSA-N oryzalin Chemical compound CCCN(CCC)C1=C([N+]([O-])=O)C=C(S(N)(=O)=O)C=C1[N+]([O-])=O UNAHYJYOSSSJHH-UHFFFAOYSA-N 0.000 description 1
- 210000001672 ovary Anatomy 0.000 description 1
- 230000008122 ovule development Effects 0.000 description 1
- 210000004681 ovum Anatomy 0.000 description 1
- 101150101567 pat-2 gene Proteins 0.000 description 1
- 230000008823 permeabilization Effects 0.000 description 1
- 230000000361 pesticidal effect Effects 0.000 description 1
- 238000002823 phage display Methods 0.000 description 1
- 125000002467 phosphate group Chemical group [H]OP(=O)(O[H])O[*] 0.000 description 1
- 150000004713 phosphodiesters Chemical group 0.000 description 1
- 230000029553 photosynthesis Effects 0.000 description 1
- 238000010672 photosynthesis Methods 0.000 description 1
- 230000000243 photosynthetic effect Effects 0.000 description 1
- 235000002949 phytic acid Nutrition 0.000 description 1
- 230000010152 pollination Effects 0.000 description 1
- 102000054765 polymorphisms of proteins Human genes 0.000 description 1
- 239000000843 powder Substances 0.000 description 1
- 239000002243 precursor Substances 0.000 description 1
- 239000003755 preservative agent Substances 0.000 description 1
- 230000002335 preservative effect Effects 0.000 description 1
- PHNUZKMIPFFYSO-UHFFFAOYSA-N propyzamide Chemical compound C#CC(C)(C)NC(=O)C1=CC(Cl)=CC(Cl)=C1 PHNUZKMIPFFYSO-UHFFFAOYSA-N 0.000 description 1
- 108020001580 protein domains Proteins 0.000 description 1
- 230000002285 radioactive effect Effects 0.000 description 1
- 238000011084 recovery Methods 0.000 description 1
- 230000001850 reproductive effect Effects 0.000 description 1
- 230000000717 retained effect Effects 0.000 description 1
- 230000002441 reversible effect Effects 0.000 description 1
- PYWVYCXTNDRMGF-UHFFFAOYSA-N rhodamine B Chemical compound [Cl-].C=12C=CC(=[N+](CC)CC)C=C2OC2=CC(N(CC)CC)=CC=C2C=1C1=CC=CC=C1C(O)=O PYWVYCXTNDRMGF-UHFFFAOYSA-N 0.000 description 1
- 108091092562 ribozyme Proteins 0.000 description 1
- 238000012216 screening Methods 0.000 description 1
- 238000010187 selection method Methods 0.000 description 1
- 230000009758 senescence Effects 0.000 description 1
- 238000012163 sequencing technique Methods 0.000 description 1
- 230000014639 sexual reproduction Effects 0.000 description 1
- 230000019491 signal transduction Effects 0.000 description 1
- 229910010271 silicon carbide Inorganic materials 0.000 description 1
- 230000005783 single-strand break Effects 0.000 description 1
- 239000004055 small Interfering RNA Substances 0.000 description 1
- 238000002791 soaking Methods 0.000 description 1
- 239000007787 solid Substances 0.000 description 1
- 238000000527 sonication Methods 0.000 description 1
- 230000002269 spontaneous effect Effects 0.000 description 1
- 238000005507 spraying Methods 0.000 description 1
- 238000003860 storage Methods 0.000 description 1
- 230000035882 stress Effects 0.000 description 1
- 239000004094 surface-active agent Substances 0.000 description 1
- 230000004083 survival effect Effects 0.000 description 1
- 239000000725 suspension Substances 0.000 description 1
- 230000002194 synthesizing effect Effects 0.000 description 1
- 235000020357 syrup Nutrition 0.000 description 1
- 239000006188 syrup Substances 0.000 description 1
- 238000012360 testing method Methods 0.000 description 1
- RYYWUUFWQRZTIU-UHFFFAOYSA-K thiophosphate Chemical group [O-]P([O-])([O-])=S RYYWUUFWQRZTIU-UHFFFAOYSA-K 0.000 description 1
- 231100000331 toxic Toxicity 0.000 description 1
- 230000002588 toxic effect Effects 0.000 description 1
- 238000001890 transfection Methods 0.000 description 1
- 230000009466 transformation Effects 0.000 description 1
- 230000001052 transient effect Effects 0.000 description 1
- 238000013519 translation Methods 0.000 description 1
- ZSDSQXJSNMTJDA-UHFFFAOYSA-N trifluralin Chemical compound CCCN(CCC)C1=C([N+]([O-])=O)C=C(C(F)(F)F)C=C1[N+]([O-])=O ZSDSQXJSNMTJDA-UHFFFAOYSA-N 0.000 description 1
- NMEHNETUFHBYEG-IHKSMFQHSA-N tttn Chemical compound C([C@@H](C(=O)N[C@@H]([C@@H](C)CC)C(=O)N[C@@H](CC=1C=CC(O)=CC=1)C(=O)N[C@@H](CO)C(=O)N[C@@H](CC=1NC=NC=1)C(=O)N[C@@H](CC=1C=CC=CC=1)C(=O)N[C@@H](CCCCN)C(=O)N[C@@H](CCC(N)=O)C(=O)N[C@@H](C)C(=O)N[C@@H]([C@@H](C)O)C(=O)N[C@@H](C(C)C)C(=O)NCC(=O)N[C@@H](CC(O)=O)C(=O)N[C@@H](C(C)C)C(=O)N[C@@H](CC(N)=O)C(=O)N[C@@H]([C@@H](C)O)C(=O)N[C@@H](CC(O)=O)C(=O)N[C@@H](CCCNC(N)=N)C(=O)N1[C@@H](CCC1)C(=O)NCC(=O)N[C@@H](CC(C)C)C(=O)N[C@@H](CC(C)C)C(=O)N[C@@H](CC(O)=O)C(=O)N[C@@H](CC(C)C)C(=O)N[C@@H](CCCCN)C(O)=O)NC(=O)[C@H](CC(C)C)NC(=O)[C@H](CCSC)NC(=O)[C@H](CCC(O)=O)NC(=O)[C@H](CCC(O)=O)NC(=O)[C@H](CC(O)=O)NC(=O)[C@@H](NC(=O)[C@H]1N(CCC1)C(=O)[C@H](CCC(N)=O)NC(=O)[C@@H](N)[C@@H](C)O)[C@@H](C)O)C1=CC=CC=C1 NMEHNETUFHBYEG-IHKSMFQHSA-N 0.000 description 1
- 238000002604 ultrasonography Methods 0.000 description 1
- 230000003827 upregulation Effects 0.000 description 1
- DRTQHJPVMGBUCF-UHFFFAOYSA-N uracil arabinoside Natural products OC1C(O)C(CO)OC1N1C(=O)NC(=O)C=C1 DRTQHJPVMGBUCF-UHFFFAOYSA-N 0.000 description 1
- 229940045145 uridine Drugs 0.000 description 1
- 108700026220 vif Genes Proteins 0.000 description 1
- 239000013603 viral vector Substances 0.000 description 1
- 239000011782 vitamin Substances 0.000 description 1
- 235000013343 vitamin Nutrition 0.000 description 1
- 229940088594 vitamin Drugs 0.000 description 1
- 229930003231 vitamin Natural products 0.000 description 1
- 150000003722 vitamin derivatives Chemical class 0.000 description 1
- 238000003260 vortexing Methods 0.000 description 1
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 description 1
- 238000001086 yeast two-hybrid system Methods 0.000 description 1
Classifications
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01H—NEW PLANTS OR NON-TRANSGENIC PROCESSES FOR OBTAINING THEM; PLANT REPRODUCTION BY TISSUE CULTURE TECHNIQUES
- A01H1/00—Processes for modifying genotypes ; Plants characterised by associated natural traits
- A01H1/04—Processes of selection involving genotypic or phenotypic markers; Methods of using phenotypic markers for selection
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
- C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
- C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
- C12N15/8201—Methods for introducing genetic material into plant cells, e.g. DNA, RNA, stable or transient incorporation, tissue culture methods adapted for transformation
- C12N15/8213—Targeted insertion of genes into the plant genome by homologous recombination
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N2310/00—Structure or type of the nucleic acid
- C12N2310/10—Type of nucleic acid
- C12N2310/20—Type of nucleic acid involving clustered regularly interspaced short palindromic repeats [CRISPRs]
-
- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02A—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE
- Y02A40/00—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production
- Y02A40/10—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production in agriculture
- Y02A40/146—Genetically Modified [GMO] plants, e.g. transgenic plants
Landscapes
- Health & Medical Sciences (AREA)
- Genetics & Genomics (AREA)
- Life Sciences & Earth Sciences (AREA)
- Engineering & Computer Science (AREA)
- Biomedical Technology (AREA)
- Biotechnology (AREA)
- Organic Chemistry (AREA)
- Wood Science & Technology (AREA)
- General Health & Medical Sciences (AREA)
- General Engineering & Computer Science (AREA)
- Chemical & Material Sciences (AREA)
- Zoology (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Physics & Mathematics (AREA)
- Botany (AREA)
- Plant Pathology (AREA)
- Molecular Biology (AREA)
- Biochemistry (AREA)
- Biophysics (AREA)
- Cell Biology (AREA)
- Microbiology (AREA)
- Developmental Biology & Embryology (AREA)
- Environmental Sciences (AREA)
- Breeding Of Plants And Reproduction By Means Of Culturing (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
- Agricultural Chemicals And Associated Chemicals (AREA)
- Cultivation Of Plants (AREA)
Abstract
L'invention divulgue des méthodes d'excision sélective de locus transgéniques à partir de plantes transgéniques et l'utilisation de ces méthodes pour faciliter la reproduction de plantes.
Applications Claiming Priority (35)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| US202063059963P | 2020-07-31 | 2020-07-31 | |
| US202063059860P | 2020-07-31 | 2020-07-31 | |
| US202063059916P | 2020-07-31 | 2020-07-31 | |
| US202063059813P | 2020-07-31 | 2020-07-31 | |
| US63/059,963 | 2020-07-31 | ||
| US63/059,860 | 2020-07-31 | ||
| US63/059,813 | 2020-07-31 | ||
| US63/059,916 | 2020-07-31 | ||
| US202163199930P | 2021-02-03 | 2021-02-03 | |
| US63/199,930 | 2021-02-03 | ||
| US202163199951P | 2021-02-04 | 2021-02-04 | |
| US202163199949P | 2021-02-04 | 2021-02-04 | |
| US63/199,949 | 2021-02-04 | ||
| US63/199,951 | 2021-02-04 | ||
| US17/248,936 | 2021-02-12 | ||
| US17/248,936 US11359210B2 (en) | 2020-07-31 | 2021-02-12 | INIR12 transgenic maize |
| US17/249,640 US11214811B1 (en) | 2020-07-31 | 2021-03-08 | INIR6 transgenic maize |
| US17/249,640 | 2021-03-08 | ||
| US202163201029P | 2021-04-09 | 2021-04-09 | |
| US202163201030P | 2021-04-09 | 2021-04-09 | |
| US63/201,030 | 2021-04-09 | ||
| US63/201,029 | 2021-04-09 | ||
| US17/302,110 US20220030822A1 (en) | 2020-07-31 | 2021-04-23 | Inht26 transgenic soybean |
| US17/302,110 | 2021-04-23 | ||
| US17/302,121 US11242534B1 (en) | 2020-07-31 | 2021-04-23 | INHT31 transgenic soybean |
| US17/302,121 | 2021-04-23 | ||
| US17/302,739 | 2021-05-11 | ||
| US17/302,739 US11326177B2 (en) | 2020-07-31 | 2021-05-11 | INIR12 transgenic maize |
| US17/303,116 US11369073B2 (en) | 2020-07-31 | 2021-05-20 | INIR12 transgenic maize |
| US17/303,116 | 2021-05-20 | ||
| US202163202569P | 2021-06-16 | 2021-06-16 | |
| US63/202,569 | 2021-06-16 | ||
| US202163203137P | 2021-07-09 | 2021-07-09 | |
| US63/203,137 | 2021-07-09 | ||
| PCT/US2021/043170 WO2022026379A1 (fr) | 2020-07-31 | 2021-07-26 | Reproduction rapide de plantes cultivées transgéniques par édition génomique |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CA3188282A1 true CA3188282A1 (fr) | 2022-02-03 |
Family
ID=85128620
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CA3188282A Pending CA3188282A1 (fr) | 2020-07-31 | 2021-07-26 | Reproduction rapide de plantes cultivees transgeniques par edition genomique |
Country Status (3)
| Country | Link |
|---|---|
| EP (1) | EP4172344A1 (fr) |
| BR (1) | BR112023001765A2 (fr) |
| CA (1) | CA3188282A1 (fr) |
-
2021
- 2021-07-26 BR BR112023001765A patent/BR112023001765A2/pt unknown
- 2021-07-26 EP EP21851339.8A patent/EP4172344A1/fr active Pending
- 2021-07-26 CA CA3188282A patent/CA3188282A1/fr active Pending
Also Published As
| Publication number | Publication date |
|---|---|
| EP4172344A1 (fr) | 2023-05-03 |
| BR112023001765A2 (pt) | 2023-03-07 |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| US11773397B2 (en) | Modified excisable DAS59122-7 maize transgenic locus | |
| CA3188280A1 (fr) | Generation de plantes avec des loci transgeniques ameliores par edition genomique | |
| US20220098602A1 (en) | Inir6 transgenic maize | |
| US11359210B2 (en) | INIR12 transgenic maize | |
| CA3188323A1 (fr) | Mais transgenique inir6 | |
| US20230313221A1 (en) | Expedited breeding of transgenic crop plants by genome editing | |
| US20230265445A1 (en) | Removable plant transgenic loci with cognate guide rna recognition sites | |
| CA3188282A1 (fr) | Reproduction rapide de plantes cultivees transgeniques par edition genomique | |
| CA3188283A1 (fr) | Edition de genome de plantes cultivees transgeniques avec des loci transgeniques modifies | |
| CA3188406A1 (fr) | Loci transgeniques de plante amovibles ayant des sites de reconnaissance d'arn guides |