CA3165815A1 - Utilisation de la chromatographie liquide et de la spectrometrie de masse pour caracteriser des oligonucleotides - Google Patents
Utilisation de la chromatographie liquide et de la spectrometrie de masse pour caracteriser des oligonucleotides Download PDFInfo
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- CA3165815A1 CA3165815A1 CA3165815A CA3165815A CA3165815A1 CA 3165815 A1 CA3165815 A1 CA 3165815A1 CA 3165815 A CA3165815 A CA 3165815A CA 3165815 A CA3165815 A CA 3165815A CA 3165815 A1 CA3165815 A1 CA 3165815A1
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- oligonucleotides
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- modification
- oligonucleotide
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- Chemical Kinetics & Catalysis (AREA)
- General Physics & Mathematics (AREA)
- Pathology (AREA)
- Other Investigation Or Analysis Of Materials By Electrical Means (AREA)
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Abstract
L'invention concerne des procédés de caractérisation d'un échantillon d'oligonucléotides d'intérêt par chromatographie liquide et spectrométrie de masse.
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US202062968368P | 2020-01-31 | 2020-01-31 | |
US62/968,368 | 2020-01-31 | ||
PCT/US2021/015697 WO2021155139A1 (fr) | 2020-01-31 | 2021-01-29 | Utilisation de la chromatographie liquide et de la spectrométrie de masse pour caractériser des oligonucléotides |
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CA3165815A1 true CA3165815A1 (fr) | 2021-08-05 |
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CA3165815A Pending CA3165815A1 (fr) | 2020-01-31 | 2021-01-29 | Utilisation de la chromatographie liquide et de la spectrometrie de masse pour caracteriser des oligonucleotides |
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US (1) | US20210239663A1 (fr) |
EP (1) | EP4097256A1 (fr) |
JP (1) | JP2023512075A (fr) |
KR (1) | KR20220136376A (fr) |
CN (1) | CN115552034A (fr) |
AU (1) | AU2021212195A1 (fr) |
BR (1) | BR112022014992A2 (fr) |
CA (1) | CA3165815A1 (fr) |
IL (1) | IL295038A (fr) |
MX (1) | MX2022009416A (fr) |
WO (1) | WO2021155139A1 (fr) |
Families Citing this family (7)
Publication number | Priority date | Publication date | Assignee | Title |
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WO2020241454A1 (fr) * | 2019-05-31 | 2020-12-03 | 株式会社新日本科学 | Procédé de spectrométrie de masse utilisant un dispositif de spectrométrie de masse par chromatographie |
US20210277383A1 (en) * | 2020-03-06 | 2021-09-09 | Waters Technologies Corporation | Purification and isolation of synthetic oligonucleotides using hydrophilic-interaction liquid chromatography |
CN114577954B (zh) * | 2022-05-09 | 2022-08-02 | 北京生物制品研究所有限责任公司 | 检测吸附型疫苗中CpG ODN含量的方法 |
US20230364585A1 (en) * | 2022-05-10 | 2023-11-16 | Waters Technologies Corporation | Method of analysis of polynucleotides by restricted access reversed phase chromatography |
CN116656781B (zh) * | 2023-07-07 | 2024-04-26 | 中国药科大学 | 一种用于检测反义寡核苷酸类药物的荧光探针及检测方法 |
CN117250301B (zh) * | 2023-11-20 | 2024-02-09 | 中国医学科学院北京协和医院 | 一种定量检测人血浆中诺西那生的液相质谱联用方法 |
CN117269402B (zh) * | 2023-11-20 | 2024-02-23 | 中国医学科学院北京协和医院 | 一种定量检测人脑脊液中诺西那生的液相质谱联用方法 |
Family Cites Families (18)
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US5585481A (en) | 1987-09-21 | 1996-12-17 | Gen-Probe Incorporated | Linking reagents for nucleotide probes |
US5378825A (en) | 1990-07-27 | 1995-01-03 | Isis Pharmaceuticals, Inc. | Backbone modified oligonucleotide analogs |
ATE515510T1 (de) | 1991-12-24 | 2011-07-15 | Isis Pharmaceuticals Inc | Durch dna-abschnitte unterbrochene modifizierte oligonukleotide |
US5605798A (en) | 1993-01-07 | 1997-02-25 | Sequenom, Inc. | DNA diagnostic based on mass spectrometry |
ES2201077T3 (es) | 1993-05-28 | 2004-03-16 | Baylor College Of Medicine | Metodo y espectrometro de masas para la desorcion e ionizacion de analitos. |
JPH10500310A (ja) | 1994-05-19 | 1998-01-13 | ダコ アクティーゼルスカブ | 淋菌及びトラコーマ クラミジアの検出のためのpna プローブ |
GB9717926D0 (en) | 1997-08-22 | 1997-10-29 | Micromass Ltd | Methods and apparatus for tandem mass spectrometry |
US6040575A (en) | 1998-01-23 | 2000-03-21 | Analytica Of Branford, Inc. | Mass spectrometry from surfaces |
US6949367B1 (en) | 1998-04-03 | 2005-09-27 | Epoch Pharmaceuticals, Inc. | Modified oligonucleotides for mismatch discrimination |
US20050144669A1 (en) | 2003-07-01 | 2005-06-30 | Whitehead Institute For Biomedical Research | MicroRNAs in plants |
US20050120415A1 (en) | 2003-10-09 | 2005-06-02 | E.I. Du Pont De Nemours And Company | Gene silencing |
US20070213292A1 (en) | 2005-08-10 | 2007-09-13 | The Rockefeller University | Chemically modified oligonucleotides for use in modulating micro RNA and uses thereof |
EP1919512B1 (fr) | 2005-08-10 | 2014-11-12 | Alnylam Pharmaceuticals Inc. | Oligonucléotides chimiquement modifiés pouvant être employés dans la modulation de micro-arn et applications |
US8148677B2 (en) | 2008-02-05 | 2012-04-03 | Thermo Finnigan Llc | Peptide identification and quantitation by merging MS/MS spectra |
US8278620B2 (en) | 2010-05-03 | 2012-10-02 | Thermo Finnigan Llc | Methods for calibration of usable fragmentation energy in mass spectrometry |
BR112016026459B1 (pt) | 2014-05-12 | 2022-09-27 | Quest Diagnostics Investments Incorporated | Métodos para determinar a quantidade de norendoxifeno e para determinar a quantidade de tamoxifeno e metabólitos do mesmo em uma amostra humana por espectrometria de massas |
US10319573B2 (en) | 2017-01-26 | 2019-06-11 | Protein Metrics Inc. | Methods and apparatuses for determining the intact mass of large molecules from mass spectrographic data |
WO2020252292A1 (fr) * | 2019-06-14 | 2020-12-17 | Laboratory Corporation Of America Holdings | Bioanalyse lc-ms d'oligonucléotides sans appariement d'ions |
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2021
- 2021-01-29 KR KR1020227029389A patent/KR20220136376A/ko unknown
- 2021-01-29 AU AU2021212195A patent/AU2021212195A1/en active Pending
- 2021-01-29 BR BR112022014992A patent/BR112022014992A2/pt unknown
- 2021-01-29 IL IL295038A patent/IL295038A/en unknown
- 2021-01-29 CA CA3165815A patent/CA3165815A1/fr active Pending
- 2021-01-29 WO PCT/US2021/015697 patent/WO2021155139A1/fr active Application Filing
- 2021-01-29 JP JP2022546402A patent/JP2023512075A/ja active Pending
- 2021-01-29 MX MX2022009416A patent/MX2022009416A/es unknown
- 2021-01-29 US US17/161,803 patent/US20210239663A1/en active Pending
- 2021-01-29 EP EP21707843.5A patent/EP4097256A1/fr active Pending
- 2021-01-29 CN CN202180016257.4A patent/CN115552034A/zh active Pending
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WO2021155139A1 (fr) | 2021-08-05 |
KR20220136376A (ko) | 2022-10-07 |
MX2022009416A (es) | 2022-10-21 |
CN115552034A (zh) | 2022-12-30 |
AU2021212195A1 (en) | 2022-09-15 |
EP4097256A1 (fr) | 2022-12-07 |
BR112022014992A2 (pt) | 2022-10-11 |
JP2023512075A (ja) | 2023-03-23 |
IL295038A (en) | 2022-09-01 |
US20210239663A1 (en) | 2021-08-05 |
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