CA3053009A1 - Optics, device, and system for assaying - Google Patents
Optics, device, and system for assayingInfo
- Publication number
- CA3053009A1 CA3053009A1 CA3053009A CA3053009A CA3053009A1 CA 3053009 A1 CA3053009 A1 CA 3053009A1 CA 3053009 A CA3053009 A CA 3053009A CA 3053009 A CA3053009 A CA 3053009A CA 3053009 A1 CA3053009 A1 CA 3053009A1
- Authority
- CA
- Canada
- Prior art keywords
- sample
- optical
- optical assembly
- light
- imaging
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Pending
Links
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WO2021011944A2 (en) * | 2019-07-18 | 2021-01-21 | Essenlix Corporation | Imaging based homogeneous assay |
EP3798614A1 (en) * | 2019-09-30 | 2021-03-31 | IAssay, Inc. | Modular multiplex analysis devices and platforms |
GB201918948D0 (en) | 2019-12-20 | 2020-02-05 | Oxford Immune Algorithmics Ltd | Portable device for imaging biological sample |
CN113155555B (zh) * | 2020-01-23 | 2023-04-11 | 天津市政工程设计研究总院有限公司 | 用于模拟混凝土管廊的镁合金模型的制作方法 |
US11768175B1 (en) | 2020-03-04 | 2023-09-26 | 10X Genomics, Inc. | Electrophoretic methods for spatial analysis |
EP4414459A2 (en) | 2020-05-22 | 2024-08-14 | 10X Genomics, Inc. | Simultaneous spatio-temporal measurement of gene expression and cellular activity |
US12031177B1 (en) | 2020-06-04 | 2024-07-09 | 10X Genomics, Inc. | Methods of enhancing spatial resolution of transcripts |
WO2021252499A1 (en) | 2020-06-08 | 2021-12-16 | 10X Genomics, Inc. | Methods of determining a surgical margin and methods of use thereof |
AU2021288090A1 (en) * | 2020-06-10 | 2023-01-19 | 10X Genomics, Inc. | Fluid delivery methods |
US10991185B1 (en) | 2020-07-20 | 2021-04-27 | Abbott Laboratories | Digital pass verification systems and methods |
EP4199810A4 (en) * | 2020-08-23 | 2024-03-13 | My-Or Diagnostics Ltd. | APPARATUS AND METHOD FOR DETERMINING HEMOGLOBIN LEVELS |
WO2022061152A2 (en) * | 2020-09-18 | 2022-03-24 | 10X Genomics, Inc. | Sample handling apparatus and fluid delivery methods |
CN112276370B (zh) * | 2020-11-27 | 2021-10-08 | 华中科技大学 | 一种基于空间光调制器的三维码激光标刻方法及*** |
WO2022119459A1 (en) * | 2020-12-03 | 2022-06-09 | Pictor Limited | Device and method of analyte detection |
AU2021409136A1 (en) | 2020-12-21 | 2023-06-29 | 10X Genomics, Inc. | Methods, compositions, and systems for capturing probes and/or barcodes |
EP4275087A1 (en) * | 2021-01-06 | 2023-11-15 | Scopgenx Private Limited | A compact portable multimodal microscopy |
WO2022178054A1 (en) * | 2021-02-18 | 2022-08-25 | Peek Technologies, Inc. | Configurable diagnostic platform systems and methods for performing chemical test assays |
CN113984759B (zh) * | 2021-09-24 | 2023-12-12 | 暨南大学 | 一种光学即时检测***、体液玻片成晶与检测方法 |
WO2024092682A1 (zh) * | 2022-11-04 | 2024-05-10 | 深圳华大智造科技股份有限公司 | 一种光学***及检测方法 |
Family Cites Families (15)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
US7372985B2 (en) * | 2003-08-15 | 2008-05-13 | Massachusetts Institute Of Technology | Systems and methods for volumetric tissue scanning microscopy |
JP2005099786A (ja) * | 2003-08-29 | 2005-04-14 | Olympus Corp | 顕微鏡の光学素子切換装置 |
DE102004034975A1 (de) * | 2004-07-16 | 2006-02-16 | Carl Zeiss Jena Gmbh | Verfahren zur Erfassung von Bildern einer Probe mit einem Mikroskop |
JP5080186B2 (ja) * | 2007-09-26 | 2012-11-21 | 富士フイルム株式会社 | 分子分析光検出方法およびそれに用いられる分子分析光検出装置、並びにサンプルプレート |
WO2012058641A2 (en) * | 2010-10-29 | 2012-05-03 | The Regents Of The University Of California | Cellscope apparatus and methods for imaging |
WO2009088930A2 (en) * | 2008-01-02 | 2009-07-16 | The Regents Of The University Of California | High numerical aperture telemicroscopy apparatus |
KR20130131408A (ko) * | 2010-12-21 | 2013-12-03 | 더 리전트 오브 더 유니버시티 오브 캘리포니아 | 모바일 장치 상의 콤팩트한 와이드필드 형광 이미징 |
US10571395B2 (en) * | 2013-07-12 | 2020-02-25 | Nowdiagnostics, Inc. | Universal rapid diagnostic test reader with trans-visual sensitivity |
RU2668332C1 (ru) * | 2013-12-12 | 2018-09-28 | Мес Медикал Электроник Системс Лтд. | Домашнее устройство тестирования |
TWI551269B (zh) * | 2014-03-21 | 2016-10-01 | 財團法人工業技術研究院 | 攜帶型分析裝置以及系統 |
TWI533025B (zh) * | 2014-07-07 | 2016-05-11 | 億觀生物科技股份有限公司 | 可攜式顯微鏡裝置 |
CN115989999A (zh) * | 2014-07-24 | 2023-04-21 | 大学健康网络 | 用于诊断目的的数据的收集和分析 |
JP2016161550A (ja) * | 2015-03-05 | 2016-09-05 | アークレイ株式会社 | 比色測定用アダプタ |
CN204439554U (zh) * | 2015-03-27 | 2015-07-01 | 华南师范大学 | 智能手机宽场荧光成像器 |
US10068145B2 (en) * | 2015-07-30 | 2018-09-04 | Fuji Xerox Co., Ltd. | Photographing system configured to hold a pill |
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