CA2948951C - Synthese d'acides nucleiques bicatenaires - Google Patents
Synthese d'acides nucleiques bicatenaires Download PDFInfo
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- CA2948951C CA2948951C CA2948951A CA2948951A CA2948951C CA 2948951 C CA2948951 C CA 2948951C CA 2948951 A CA2948951 A CA 2948951A CA 2948951 A CA2948951 A CA 2948951A CA 2948951 C CA2948951 C CA 2948951C
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6806—Preparing nucleic acids for analysis, e.g. for polymerase chain reaction [PCR] assay
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
- C12N15/1034—Isolating an individual clone by screening libraries
- C12N15/1093—General methods of preparing gene libraries, not provided for in other subgroups
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
- C12N15/1096—Processes for the isolation, preparation or purification of DNA or RNA cDNA Synthesis; Subtracted cDNA library construction, e.g. RT, RT-PCR
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q2521/00—Reaction characterised by the enzymatic activity
- C12Q2521/10—Nucleotidyl transfering
- C12Q2521/107—RNA dependent DNA polymerase,(i.e. reverse transcriptase)
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q2525/00—Reactions involving modified oligonucleotides, nucleic acids, or nucleotides
- C12Q2525/10—Modifications characterised by
- C12Q2525/121—Modifications characterised by incorporating both deoxyribonucleotides and ribonucleotides
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q2525/00—Reactions involving modified oligonucleotides, nucleic acids, or nucleotides
- C12Q2525/10—Modifications characterised by
- C12Q2525/155—Modifications characterised by incorporating/generating a new priming site
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q2525/00—Reactions involving modified oligonucleotides, nucleic acids, or nucleotides
- C12Q2525/10—Modifications characterised by
- C12Q2525/161—Modifications characterised by incorporating target specific and non-target specific sites
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q2525/00—Reactions involving modified oligonucleotides, nucleic acids, or nucleotides
- C12Q2525/10—Modifications characterised by
- C12Q2525/173—Modifications characterised by incorporating a polynucleotide run, e.g. polyAs, polyTs
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q2525/00—Reactions involving modified oligonucleotides, nucleic acids, or nucleotides
- C12Q2525/10—Modifications characterised by
- C12Q2525/191—Modifications characterised by incorporating an adaptor
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q2531/00—Reactions of nucleic acids characterised by
- C12Q2531/10—Reactions of nucleic acids characterised by the purpose being amplify/increase the copy number of target nucleic acid
- C12Q2531/113—PCR
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q2535/00—Reactions characterised by the assay type for determining the identity of a nucleotide base or a sequence of oligonucleotides
- C12Q2535/122—Massive parallel sequencing
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- Chemical & Material Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Health & Medical Sciences (AREA)
- Genetics & Genomics (AREA)
- Engineering & Computer Science (AREA)
- Organic Chemistry (AREA)
- Zoology (AREA)
- Wood Science & Technology (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Biotechnology (AREA)
- General Engineering & Computer Science (AREA)
- Biomedical Technology (AREA)
- Molecular Biology (AREA)
- Biochemistry (AREA)
- Microbiology (AREA)
- General Health & Medical Sciences (AREA)
- Biophysics (AREA)
- Physics & Mathematics (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Analytical Chemistry (AREA)
- Plant Pathology (AREA)
- Crystallography & Structural Chemistry (AREA)
- Bioinformatics & Computational Biology (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Immunology (AREA)
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
Abstract
La présente invention concerne un procédé pour la synthèse d'acides nucléiques bicaténaires à partir d'une large variété d'échantillons et comprend l'utilisation de ces acides nucléiques pour une analyse approfondie des séquences. De plus, la présente invention concerne des réactifs spécifiques utilisés dans le procédé de la présente invention. En outre, l'invention concerne des kits comprenant des réactifs pour le procédé de l'invention et l'utilisation desdits kits.
Applications Claiming Priority (3)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
EP14168313 | 2014-05-14 | ||
EP14168313.6 | 2014-05-14 | ||
PCT/EP2015/060777 WO2015173402A1 (fr) | 2014-05-14 | 2015-05-15 | Synthèse d'acides nucléiques bicaténaires |
Publications (2)
Publication Number | Publication Date |
---|---|
CA2948951A1 CA2948951A1 (fr) | 2015-11-19 |
CA2948951C true CA2948951C (fr) | 2023-04-04 |
Family
ID=50687416
Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
CA2948951A Active CA2948951C (fr) | 2014-05-14 | 2015-05-15 | Synthese d'acides nucleiques bicatenaires |
Country Status (7)
Country | Link |
---|---|
US (1) | US10988795B2 (fr) |
EP (1) | EP3143139B1 (fr) |
CN (1) | CN106460052B (fr) |
AU (1) | AU2015261440A1 (fr) |
CA (1) | CA2948951C (fr) |
EA (1) | EA035092B1 (fr) |
WO (1) | WO2015173402A1 (fr) |
Families Citing this family (17)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
EP2912197B1 (fr) * | 2012-10-24 | 2019-08-07 | Takara Bio USA, Inc. | Procédés basés sur la commutation de matrice destinés à produire un acide nucléique de synthèse |
EP4389888A2 (fr) | 2013-10-17 | 2024-06-26 | Takara Bio USA, Inc. | Procédés d'ajout d'adaptateurs à des acides nucléiques et compositions pour leur mise en uvre |
US9719136B2 (en) | 2013-12-17 | 2017-08-01 | Takara Bio Usa, Inc. | Methods for adding adapters to nucleic acids and compositions for practicing the same |
US11098304B2 (en) | 2015-11-04 | 2021-08-24 | Atreca, Inc. | Combinatorial sets of nucleic acid barcodes for analysis of nucleic acids associated with single cells |
KR102489902B1 (ko) * | 2016-11-11 | 2023-01-19 | 바이오 래드 래버러토리스 인코오포레이티드 | 핵산 샘플을 프로세싱하는 방법 |
WO2018191563A1 (fr) * | 2017-04-12 | 2018-10-18 | Karius, Inc. | Systèmes, procédés et compositions de préparation d'échantillon |
CN110551794B (zh) * | 2018-06-04 | 2023-05-30 | 完整基因有限公司 | 对rna分子进行处理的方法及试剂盒和复合体 |
AU2019282812A1 (en) * | 2018-06-07 | 2020-12-17 | Arc Bio, Llc | Compositions and methods for making guide nucleic acids |
DK3810811T3 (da) * | 2018-06-20 | 2022-08-22 | Pathoquest | Fremgangsmåde til skelnen mellem levende og døde mikrober i en prøve |
US10696994B2 (en) | 2018-09-28 | 2020-06-30 | Bioo Scientific Corporation | Size selection of RNA using poly(A) polymerase |
WO2020069791A1 (fr) * | 2018-10-02 | 2020-04-09 | Max-Delbrück-Centrum Für Molekulare Medizin In Der Helmholtz-Gemeinschaft | Séquençage d'arn pleine longueur |
EP3798318A1 (fr) | 2019-09-30 | 2021-03-31 | Diagenode S.A. | Procédé et kit de séquençage à haut débit |
US20220177964A1 (en) | 2019-03-27 | 2022-06-09 | Diagenode S.A. | A high throughput sequencing method and kit |
CN110055316A (zh) * | 2019-03-28 | 2019-07-26 | 天津大学 | 一种用于检测微小rna的由末端脱氧核苷酸转移酶介导的反转录pcr方法 |
WO2020227654A1 (fr) * | 2019-05-09 | 2020-11-12 | Pacific Biosciences Of California, Inc. | Compositions et méthodes pour la synthèse d'adnc |
US11788137B2 (en) | 2019-09-30 | 2023-10-17 | Diagenode S.A. | Diagnostic and/or sequencing method and kit |
EP3828283A1 (fr) | 2019-11-28 | 2021-06-02 | Diagenode S.A. | Procédé et kit de séquençage améliorés |
Family Cites Families (24)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
US4661450A (en) * | 1983-05-03 | 1987-04-28 | Molecular Genetics Research And Development Limited Partnership | Molecular cloning of RNA using RNA ligase and synthetic oligonucleotides |
US6582908B2 (en) * | 1990-12-06 | 2003-06-24 | Affymetrix, Inc. | Oligonucleotides |
US5962271A (en) * | 1996-01-03 | 1999-10-05 | Cloutech Laboratories, Inc. | Methods and compositions for generating full-length cDNA having arbitrary nucleotide sequence at the 3'-end |
KR20010085901A (ko) * | 1998-10-07 | 2001-09-07 | 릴리 엠 씨즐러 스피허, 아네뜨 워너 | 식물에서의 치료학적 활성 단백질 |
DE10035084A1 (de) * | 2000-07-17 | 2002-02-07 | Aventis Cropscience Gmbh | Nucleinsäuremolekül, das für eine pflanzliche AMP-Deaminase codiert |
SE0100412D0 (sv) * | 2001-02-09 | 2001-02-09 | Anders Virtanen | Novel human poly(A) polymerase |
EP2021503A1 (fr) * | 2006-03-17 | 2009-02-11 | Solexa Ltd. | Procédés isothermiques pour créer des réseaux moléculaires clonales simples |
US8314220B2 (en) * | 2007-01-26 | 2012-11-20 | Agilent Technologies, Inc. | Methods compositions, and kits for detection of microRNA |
US20100062494A1 (en) * | 2008-08-08 | 2010-03-11 | President And Fellows Of Harvard College | Enzymatic oligonucleotide pre-adenylation |
CN101948811B (zh) * | 2010-08-18 | 2013-01-09 | 中国农业科学院兰州兽医研究所 | 用反向遗传操作拓展***疫苗株抗原谱及疫苗制备方法 |
WO2012054933A2 (fr) * | 2010-10-22 | 2012-04-26 | Fluidigm Corporation | Méthodes de dosage par sondes universelles |
GB201106254D0 (en) * | 2011-04-13 | 2011-05-25 | Frisen Jonas | Method and product |
CN102154505B (zh) | 2011-04-20 | 2013-03-20 | 苟德明 | 一种检测miRNA的方法及引物及其应用 |
US8722585B2 (en) * | 2011-05-08 | 2014-05-13 | Yan Wang | Methods of making di-tagged DNA libraries from DNA or RNA using double-tagged oligonucleotides |
US20140272998A1 (en) | 2011-07-15 | 2014-09-18 | Leo Pharma A/S | Diagnostic microrna profiling in cutaneous t-cell lymphoma (ctcl) |
US9657360B2 (en) * | 2011-09-13 | 2017-05-23 | Commonwealth Scientific And Industrial Research Organisation | Detection of viral infection |
PL3363901T3 (pl) * | 2012-02-17 | 2021-07-05 | Fred Hutchinson Cancer Research Center | Kompozycje i sposoby dokładnej identyfikacji mutacji |
CN102676677B (zh) * | 2012-05-16 | 2013-04-10 | 北京旷博生物技术有限公司 | 一种microRNA定量检测方法 |
US20140094383A1 (en) * | 2012-10-02 | 2014-04-03 | Ohio State Innovation Foundation | Tethered Lipoplex nanoparticle Biochips And Methods Of Use |
WO2014060483A1 (fr) * | 2012-10-17 | 2014-04-24 | Spatial Transcriptomics Ab | Procédés et produit d'optimisation de la détection localisée ou spatiale de l'expression génique dans un échantillon de tissu |
EP2912197B1 (fr) | 2012-10-24 | 2019-08-07 | Takara Bio USA, Inc. | Procédés basés sur la commutation de matrice destinés à produire un acide nucléique de synthèse |
US20140242581A1 (en) | 2013-01-23 | 2014-08-28 | Reproductive Genetics And Technology Solutions, Llc | Compositions and methods for genetic analysis of embryos |
EP4389888A2 (fr) | 2013-10-17 | 2024-06-26 | Takara Bio USA, Inc. | Procédés d'ajout d'adaptateurs à des acides nucléiques et compositions pour leur mise en uvre |
WO2018035364A1 (fr) * | 2016-08-17 | 2018-02-22 | The Broad Institute Inc. | Produit et procédés utiles pour moduler et évaluer des réponses immunitaires |
-
2015
- 2015-05-15 CA CA2948951A patent/CA2948951C/fr active Active
- 2015-05-15 AU AU2015261440A patent/AU2015261440A1/en not_active Abandoned
- 2015-05-15 EA EA201692157A patent/EA035092B1/ru not_active IP Right Cessation
- 2015-05-15 US US15/309,237 patent/US10988795B2/en active Active
- 2015-05-15 EP EP15724585.3A patent/EP3143139B1/fr active Active
- 2015-05-15 CN CN201580024928.6A patent/CN106460052B/zh active Active
- 2015-05-15 WO PCT/EP2015/060777 patent/WO2015173402A1/fr active Application Filing
Also Published As
Publication number | Publication date |
---|---|
EA035092B1 (ru) | 2020-04-27 |
EA201692157A1 (ru) | 2017-05-31 |
EP3143139B1 (fr) | 2021-12-15 |
CN106460052A (zh) | 2017-02-22 |
WO2015173402A1 (fr) | 2015-11-19 |
CN106460052B (zh) | 2021-07-16 |
CA2948951A1 (fr) | 2015-11-19 |
AU2015261440A1 (en) | 2017-01-05 |
US10988795B2 (en) | 2021-04-27 |
US20190032110A1 (en) | 2019-01-31 |
EP3143139A1 (fr) | 2017-03-22 |
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