CA2948951C - Synthese d'acides nucleiques bicatenaires - Google Patents

Synthese d'acides nucleiques bicatenaires Download PDF

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CA2948951C
CA2948951C CA2948951A CA2948951A CA2948951C CA 2948951 C CA2948951 C CA 2948951C CA 2948951 A CA2948951 A CA 2948951A CA 2948951 A CA2948951 A CA 2948951A CA 2948951 C CA2948951 C CA 2948951C
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seq
dna
nucleotides
nucleic acid
sequence
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CA2948951A1 (fr
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Andrey TURCHINOVICH
Harald SUROWY
Barbara BURWINKEL
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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6806—Preparing nucleic acids for analysis, e.g. for polymerase chain reaction [PCR] assay
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
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    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/1034—Isolating an individual clone by screening libraries
    • C12N15/1093—General methods of preparing gene libraries, not provided for in other subgroups
    • C—CHEMISTRY; METALLURGY
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    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/1096—Processes for the isolation, preparation or purification of DNA or RNA cDNA Synthesis; Subtracted cDNA library construction, e.g. RT, RT-PCR
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2521/00—Reaction characterised by the enzymatic activity
    • C12Q2521/10—Nucleotidyl transfering
    • C12Q2521/107—RNA dependent DNA polymerase,(i.e. reverse transcriptase)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2525/00—Reactions involving modified oligonucleotides, nucleic acids, or nucleotides
    • C12Q2525/10—Modifications characterised by
    • C12Q2525/121—Modifications characterised by incorporating both deoxyribonucleotides and ribonucleotides
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
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    • C12Q2525/00—Reactions involving modified oligonucleotides, nucleic acids, or nucleotides
    • C12Q2525/10—Modifications characterised by
    • C12Q2525/155—Modifications characterised by incorporating/generating a new priming site
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2525/00—Reactions involving modified oligonucleotides, nucleic acids, or nucleotides
    • C12Q2525/10—Modifications characterised by
    • C12Q2525/161—Modifications characterised by incorporating target specific and non-target specific sites
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2525/00—Reactions involving modified oligonucleotides, nucleic acids, or nucleotides
    • C12Q2525/10—Modifications characterised by
    • C12Q2525/173—Modifications characterised by incorporating a polynucleotide run, e.g. polyAs, polyTs
    • C—CHEMISTRY; METALLURGY
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    • C12Q2525/00—Reactions involving modified oligonucleotides, nucleic acids, or nucleotides
    • C12Q2525/10—Modifications characterised by
    • C12Q2525/191—Modifications characterised by incorporating an adaptor
    • C—CHEMISTRY; METALLURGY
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    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2531/00—Reactions of nucleic acids characterised by
    • C12Q2531/10—Reactions of nucleic acids characterised by the purpose being amplify/increase the copy number of target nucleic acid
    • C12Q2531/113—PCR
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2535/00—Reactions characterised by the assay type for determining the identity of a nucleotide base or a sequence of oligonucleotides
    • C12Q2535/122—Massive parallel sequencing

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  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Engineering & Computer Science (AREA)
  • Organic Chemistry (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biotechnology (AREA)
  • General Engineering & Computer Science (AREA)
  • Biomedical Technology (AREA)
  • Molecular Biology (AREA)
  • Biochemistry (AREA)
  • Microbiology (AREA)
  • General Health & Medical Sciences (AREA)
  • Biophysics (AREA)
  • Physics & Mathematics (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Analytical Chemistry (AREA)
  • Plant Pathology (AREA)
  • Crystallography & Structural Chemistry (AREA)
  • Bioinformatics & Computational Biology (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Immunology (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)

Abstract

La présente invention concerne un procédé pour la synthèse d'acides nucléiques bicaténaires à partir d'une large variété d'échantillons et comprend l'utilisation de ces acides nucléiques pour une analyse approfondie des séquences. De plus, la présente invention concerne des réactifs spécifiques utilisés dans le procédé de la présente invention. En outre, l'invention concerne des kits comprenant des réactifs pour le procédé de l'invention et l'utilisation desdits kits.
CA2948951A 2014-05-14 2015-05-15 Synthese d'acides nucleiques bicatenaires Active CA2948951C (fr)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
EP14168313 2014-05-14
EP14168313.6 2014-05-14
PCT/EP2015/060777 WO2015173402A1 (fr) 2014-05-14 2015-05-15 Synthèse d'acides nucléiques bicaténaires

Publications (2)

Publication Number Publication Date
CA2948951A1 CA2948951A1 (fr) 2015-11-19
CA2948951C true CA2948951C (fr) 2023-04-04

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Family Applications (1)

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CA2948951A Active CA2948951C (fr) 2014-05-14 2015-05-15 Synthese d'acides nucleiques bicatenaires

Country Status (7)

Country Link
US (1) US10988795B2 (fr)
EP (1) EP3143139B1 (fr)
CN (1) CN106460052B (fr)
AU (1) AU2015261440A1 (fr)
CA (1) CA2948951C (fr)
EA (1) EA035092B1 (fr)
WO (1) WO2015173402A1 (fr)

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EP2912197B1 (fr) * 2012-10-24 2019-08-07 Takara Bio USA, Inc. Procédés basés sur la commutation de matrice destinés à produire un acide nucléique de synthèse
EP4389888A2 (fr) 2013-10-17 2024-06-26 Takara Bio USA, Inc. Procédés d'ajout d'adaptateurs à des acides nucléiques et compositions pour leur mise en uvre
US9719136B2 (en) 2013-12-17 2017-08-01 Takara Bio Usa, Inc. Methods for adding adapters to nucleic acids and compositions for practicing the same
US11098304B2 (en) 2015-11-04 2021-08-24 Atreca, Inc. Combinatorial sets of nucleic acid barcodes for analysis of nucleic acids associated with single cells
KR102489902B1 (ko) * 2016-11-11 2023-01-19 바이오 래드 래버러토리스 인코오포레이티드 핵산 샘플을 프로세싱하는 방법
WO2018191563A1 (fr) * 2017-04-12 2018-10-18 Karius, Inc. Systèmes, procédés et compositions de préparation d'échantillon
CN110551794B (zh) * 2018-06-04 2023-05-30 完整基因有限公司 对rna分子进行处理的方法及试剂盒和复合体
AU2019282812A1 (en) * 2018-06-07 2020-12-17 Arc Bio, Llc Compositions and methods for making guide nucleic acids
DK3810811T3 (da) * 2018-06-20 2022-08-22 Pathoquest Fremgangsmåde til skelnen mellem levende og døde mikrober i en prøve
US10696994B2 (en) 2018-09-28 2020-06-30 Bioo Scientific Corporation Size selection of RNA using poly(A) polymerase
WO2020069791A1 (fr) * 2018-10-02 2020-04-09 Max-Delbrück-Centrum Für Molekulare Medizin In Der Helmholtz-Gemeinschaft Séquençage d'arn pleine longueur
EP3798318A1 (fr) 2019-09-30 2021-03-31 Diagenode S.A. Procédé et kit de séquençage à haut débit
US20220177964A1 (en) 2019-03-27 2022-06-09 Diagenode S.A. A high throughput sequencing method and kit
CN110055316A (zh) * 2019-03-28 2019-07-26 天津大学 一种用于检测微小rna的由末端脱氧核苷酸转移酶介导的反转录pcr方法
WO2020227654A1 (fr) * 2019-05-09 2020-11-12 Pacific Biosciences Of California, Inc. Compositions et méthodes pour la synthèse d'adnc
US11788137B2 (en) 2019-09-30 2023-10-17 Diagenode S.A. Diagnostic and/or sequencing method and kit
EP3828283A1 (fr) 2019-11-28 2021-06-02 Diagenode S.A. Procédé et kit de séquençage améliorés

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Also Published As

Publication number Publication date
EA035092B1 (ru) 2020-04-27
EA201692157A1 (ru) 2017-05-31
EP3143139B1 (fr) 2021-12-15
CN106460052A (zh) 2017-02-22
WO2015173402A1 (fr) 2015-11-19
CN106460052B (zh) 2021-07-16
CA2948951A1 (fr) 2015-11-19
AU2015261440A1 (en) 2017-01-05
US10988795B2 (en) 2021-04-27
US20190032110A1 (en) 2019-01-31
EP3143139A1 (fr) 2017-03-22

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