CA2926427C - Procedes d'identification de champignons - Google Patents
Procedes d'identification de champignons Download PDFInfo
- Publication number
- CA2926427C CA2926427C CA2926427A CA2926427A CA2926427C CA 2926427 C CA2926427 C CA 2926427C CA 2926427 A CA2926427 A CA 2926427A CA 2926427 A CA2926427 A CA 2926427A CA 2926427 C CA2926427 C CA 2926427C
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- 238000000034 method Methods 0.000 title claims abstract description 131
- 241000233866 Fungi Species 0.000 title claims description 79
- 230000002538 fungal effect Effects 0.000 claims abstract description 172
- 150000002632 lipids Chemical class 0.000 claims abstract description 135
- 229930182558 Sterol Natural products 0.000 claims abstract description 48
- 150000003432 sterols Chemical class 0.000 claims abstract description 48
- 235000003702 sterols Nutrition 0.000 claims abstract description 48
- 150000002327 glycerophospholipids Chemical class 0.000 claims abstract description 45
- 150000003408 sphingolipids Chemical class 0.000 claims abstract description 45
- 239000012528 membrane Substances 0.000 claims abstract description 22
- 150000002500 ions Chemical class 0.000 claims description 258
- 238000001228 spectrum Methods 0.000 claims description 215
- 239000002243 precursor Substances 0.000 claims description 118
- 238000001819 mass spectrum Methods 0.000 claims description 87
- 230000007935 neutral effect Effects 0.000 claims description 54
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- 230000005593 dissociations Effects 0.000 claims description 19
- 108090000623 proteins and genes Proteins 0.000 claims description 15
- 102000004169 proteins and genes Human genes 0.000 claims description 15
- 238000010504 bond cleavage reaction Methods 0.000 claims description 13
- UHPMCKVQTMMPCG-UHFFFAOYSA-N 5,8-dihydroxy-2-methoxy-6-methyl-7-(2-oxopropyl)naphthalene-1,4-dione Chemical compound CC1=C(CC(C)=O)C(O)=C2C(=O)C(OC)=CC(=O)C2=C1O UHPMCKVQTMMPCG-UHFFFAOYSA-N 0.000 claims description 10
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- 241000222120 Candida <Saccharomycetales> Species 0.000 claims description 8
- 241001337994 Cryptococcus <scale insect> Species 0.000 claims description 8
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- 241000123650 Botrytis cinerea Species 0.000 claims description 5
- 241000222122 Candida albicans Species 0.000 claims description 5
- 241000223682 Exophiala Species 0.000 claims description 5
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- 241000228402 Histoplasma Species 0.000 claims description 5
- 241000228404 Histoplasma capsulatum Species 0.000 claims description 5
- 241001126842 Ichthyosporidium Species 0.000 claims description 5
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- 241001149962 Sporothrix Species 0.000 claims description 5
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- 241000082085 Verticillium <Phyllachorales> Species 0.000 claims description 5
- 229940095731 candida albicans Drugs 0.000 claims description 5
- 201000000317 pneumocystosis Diseases 0.000 claims description 5
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- 241000682645 Phakopsora pachyrhizi Species 0.000 claims description 4
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- OKKJLVBELUTLKV-UHFFFAOYSA-N Methanol Chemical compound OC OKKJLVBELUTLKV-UHFFFAOYSA-N 0.000 description 15
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- 239000000194 fatty acid Substances 0.000 description 15
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- HEDRZPFGACZZDS-UHFFFAOYSA-N Chloroform Chemical compound ClC(Cl)Cl HEDRZPFGACZZDS-UHFFFAOYSA-N 0.000 description 8
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- 238000013500 data storage Methods 0.000 description 8
- 238000001840 matrix-assisted laser desorption--ionisation time-of-flight mass spectrometry Methods 0.000 description 8
- OKTJSMMVPCPJKN-UHFFFAOYSA-N Carbon Chemical compound [C] OKTJSMMVPCPJKN-UHFFFAOYSA-N 0.000 description 7
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- KQNPFQTWMSNSAP-UHFFFAOYSA-N isobutyric acid Chemical compound CC(C)C(O)=O KQNPFQTWMSNSAP-UHFFFAOYSA-N 0.000 description 6
- 238000002955 isolation Methods 0.000 description 6
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- 230000000843 anti-fungal effect Effects 0.000 description 5
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- 239000011159 matrix material Substances 0.000 description 5
- NBIIXXVUZAFLBC-UHFFFAOYSA-K phosphate Chemical compound [O-]P([O-])([O-])=O NBIIXXVUZAFLBC-UHFFFAOYSA-K 0.000 description 5
- 239000010452 phosphate Substances 0.000 description 5
- -1 reservoirs Substances 0.000 description 5
- 108010026552 Proteome Proteins 0.000 description 4
- 238000004422 calculation algorithm Methods 0.000 description 4
- 238000000132 electrospray ionisation Methods 0.000 description 4
- 244000053095 fungal pathogen Species 0.000 description 4
- BDAGIHXWWSANSR-UHFFFAOYSA-N methanoic acid Natural products OC=O BDAGIHXWWSANSR-UHFFFAOYSA-N 0.000 description 4
- 238000011160 research Methods 0.000 description 4
- 238000010897 surface acoustic wave method Methods 0.000 description 4
- WEVYAHXRMPXWCK-UHFFFAOYSA-N Acetonitrile Chemical compound CC#N WEVYAHXRMPXWCK-UHFFFAOYSA-N 0.000 description 3
- VHUUQVKOLVNVRT-UHFFFAOYSA-N Ammonium hydroxide Chemical compound [NH4+].[OH-] VHUUQVKOLVNVRT-UHFFFAOYSA-N 0.000 description 3
- 208000011380 COVID-19–associated multisystem inflammatory syndrome in children Diseases 0.000 description 3
- VMHLLURERBWHNL-UHFFFAOYSA-M Sodium acetate Chemical compound [Na+].CC([O-])=O VMHLLURERBWHNL-UHFFFAOYSA-M 0.000 description 3
- 239000000908 ammonium hydroxide Substances 0.000 description 3
- 238000004364 calculation method Methods 0.000 description 3
- 210000002421 cell wall Anatomy 0.000 description 3
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- 238000000534 ion trap mass spectrometry Methods 0.000 description 3
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- 239000001632 sodium acetate Substances 0.000 description 3
- 235000017281 sodium acetate Nutrition 0.000 description 3
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- OSWFIVFLDKOXQC-UHFFFAOYSA-N 4-(3-methoxyphenyl)aniline Chemical group COC1=CC=CC(C=2C=CC(N)=CC=2)=C1 OSWFIVFLDKOXQC-UHFFFAOYSA-N 0.000 description 2
- LFQSCWFLJHTTHZ-UHFFFAOYSA-N Ethanol Chemical compound CCO LFQSCWFLJHTTHZ-UHFFFAOYSA-N 0.000 description 2
- SUHOOTKUPISOBE-UHFFFAOYSA-N O-phosphoethanolamine Chemical compound NCCOP(O)(O)=O SUHOOTKUPISOBE-UHFFFAOYSA-N 0.000 description 2
- ISWSIDIOOBJBQZ-UHFFFAOYSA-N Phenol Chemical compound OC1=CC=CC=C1 ISWSIDIOOBJBQZ-UHFFFAOYSA-N 0.000 description 2
- 125000002252 acyl group Chemical group 0.000 description 2
- 229940121375 antifungal agent Drugs 0.000 description 2
- 239000000090 biomarker Substances 0.000 description 2
- 150000001720 carbohydrates Chemical group 0.000 description 2
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- YHHSONZFOIEMCP-UHFFFAOYSA-O phosphocholine Chemical compound C[N+](C)(C)CCOP(O)(O)=O YHHSONZFOIEMCP-UHFFFAOYSA-O 0.000 description 2
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- GQYHUHYESMUTHG-UHFFFAOYSA-N lithium niobate Chemical compound [Li+].[O-][Nb](=O)=O GQYHUHYESMUTHG-UHFFFAOYSA-N 0.000 description 1
- 238000012423 maintenance Methods 0.000 description 1
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- 125000002467 phosphate group Chemical group [H]OP(=O)(O[H])O[*] 0.000 description 1
- 150000003904 phospholipids Chemical group 0.000 description 1
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- AIFRHYZBTHREPW-UHFFFAOYSA-N β-carboline Chemical compound N1=CC=C2C3=CC=CC=C3NC2=C1 AIFRHYZBTHREPW-UHFFFAOYSA-N 0.000 description 1
Classifications
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/02—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving viable microorganisms
- C12Q1/04—Determining presence or kind of microorganism; Use of selective media for testing antibiotics or bacteriocides; Compositions containing a chemical indicator therefor
-
- G—PHYSICS
- G16—INFORMATION AND COMMUNICATION TECHNOLOGY [ICT] SPECIALLY ADAPTED FOR SPECIFIC APPLICATION FIELDS
- G16C—COMPUTATIONAL CHEMISTRY; CHEMOINFORMATICS; COMPUTATIONAL MATERIALS SCIENCE
- G16C20/00—Chemoinformatics, i.e. ICT specially adapted for the handling of physicochemical or structural data of chemical particles, elements, compounds or mixtures
- G16C20/60—In silico combinatorial chemistry
- G16C20/62—Design of libraries
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
- G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
- G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
- G01N33/569—Immunoassay; Biospecific binding assay; Materials therefor for microorganisms, e.g. protozoa, bacteria, viruses
- G01N33/56961—Plant cells or fungi
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
- G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
- G01N33/92—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving lipids, e.g. cholesterol, lipoproteins, or their receptors
-
- G—PHYSICS
- G16—INFORMATION AND COMMUNICATION TECHNOLOGY [ICT] SPECIALLY ADAPTED FOR SPECIFIC APPLICATION FIELDS
- G16B—BIOINFORMATICS, i.e. INFORMATION AND COMMUNICATION TECHNOLOGY [ICT] SPECIALLY ADAPTED FOR GENETIC OR PROTEIN-RELATED DATA PROCESSING IN COMPUTATIONAL MOLECULAR BIOLOGY
- G16B35/00—ICT specially adapted for in silico combinatorial libraries of nucleic acids, proteins or peptides
-
- G—PHYSICS
- G16—INFORMATION AND COMMUNICATION TECHNOLOGY [ICT] SPECIALLY ADAPTED FOR SPECIFIC APPLICATION FIELDS
- G16C—COMPUTATIONAL CHEMISTRY; CHEMOINFORMATICS; COMPUTATIONAL MATERIALS SCIENCE
- G16C20/00—Chemoinformatics, i.e. ICT specially adapted for the handling of physicochemical or structural data of chemical particles, elements, compounds or mixtures
- G16C20/20—Identification of molecular entities, parts thereof or of chemical compositions
-
- G—PHYSICS
- G16—INFORMATION AND COMMUNICATION TECHNOLOGY [ICT] SPECIALLY ADAPTED FOR SPECIFIC APPLICATION FIELDS
- G16C—COMPUTATIONAL CHEMISTRY; CHEMOINFORMATICS; COMPUTATIONAL MATERIALS SCIENCE
- G16C20/00—Chemoinformatics, i.e. ICT specially adapted for the handling of physicochemical or structural data of chemical particles, elements, compounds or mixtures
- G16C20/60—In silico combinatorial chemistry
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N2333/00—Assays involving biological materials from specific organisms or of a specific nature
- G01N2333/37—Assays involving biological materials from specific organisms or of a specific nature from fungi
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N2560/00—Chemical aspects of mass spectrometric analysis of biological material
Landscapes
- Life Sciences & Earth Sciences (AREA)
- Health & Medical Sciences (AREA)
- Engineering & Computer Science (AREA)
- Chemical & Material Sciences (AREA)
- Molecular Biology (AREA)
- General Health & Medical Sciences (AREA)
- Immunology (AREA)
- Physics & Mathematics (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Urology & Nephrology (AREA)
- Hematology (AREA)
- Biomedical Technology (AREA)
- Medicinal Chemistry (AREA)
- Biochemistry (AREA)
- Biotechnology (AREA)
- Microbiology (AREA)
- Theoretical Computer Science (AREA)
- Bioinformatics & Computational Biology (AREA)
- Cell Biology (AREA)
- Biophysics (AREA)
- Analytical Chemistry (AREA)
- Computing Systems (AREA)
- Crystallography & Structural Chemistry (AREA)
- Food Science & Technology (AREA)
- General Physics & Mathematics (AREA)
- Pathology (AREA)
- Library & Information Science (AREA)
- Organic Chemistry (AREA)
- Spectroscopy & Molecular Physics (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Zoology (AREA)
- Wood Science & Technology (AREA)
- Medical Informatics (AREA)
- Evolutionary Biology (AREA)
- Endocrinology (AREA)
- Tropical Medicine & Parasitology (AREA)
- Virology (AREA)
- Mycology (AREA)
- Botany (AREA)
- General Engineering & Computer Science (AREA)
Abstract
L'invention concerne des procédés qui permettent d'identifier des espèces fongiques par analyse de lipides de membrane fongique, tels que des glycérophospholipides, des sphingolipides et des stérols, à l'aide de motifs d'ionisation de spectrométrie de masse.
Applications Claiming Priority (3)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| US201361888577P | 2013-10-09 | 2013-10-09 | |
| US61/888,577 | 2013-10-09 | ||
| PCT/US2014/059853 WO2015054468A1 (fr) | 2013-10-09 | 2014-10-09 | Procédés d'identification de champignons |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CA2926427A1 CA2926427A1 (fr) | 2015-04-16 |
| CA2926427C true CA2926427C (fr) | 2024-06-04 |
Family
ID=52813634
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CA2926427A Active CA2926427C (fr) | 2013-10-09 | 2014-10-09 | Procedes d'identification de champignons |
Country Status (6)
| Country | Link |
|---|---|
| US (1) | US10465223B2 (fr) |
| EP (1) | EP3055421A4 (fr) |
| JP (1) | JP2016537964A (fr) |
| AU (1) | AU2014331866A1 (fr) |
| CA (1) | CA2926427C (fr) |
| WO (1) | WO2015054468A1 (fr) |
Families Citing this family (21)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US20170370849A1 (en) * | 2014-12-12 | 2017-12-28 | Nitin Shashikant CHOUTHAI | Methods to identify candida species from patient cultures using raman spectrometry |
| CN107645938B (zh) | 2015-03-06 | 2020-11-20 | 英国质谱公司 | 成像导引的敞开式电离质谱测定 |
| EP3264990B1 (fr) | 2015-03-06 | 2022-01-19 | Micromass UK Limited | Appareil pour effectuer la spectrométrie de masse à ionisation rapide par évaporation |
| GB2553918B (en) | 2015-03-06 | 2022-10-12 | Micromass Ltd | Ambient ionization mass spectrometry imaging platform for direct mapping from bulk tissue |
| US11239066B2 (en) | 2015-03-06 | 2022-02-01 | Micromass Uk Limited | Cell population analysis |
| EP3265821B1 (fr) | 2015-03-06 | 2021-06-16 | Micromass UK Limited | Séparateur ou piège à liquide pour applications électro-chirurgicales |
| EP3265819B1 (fr) * | 2015-03-06 | 2020-10-14 | Micromass UK Limited | Spectrométrie de masse à ionisation ambiante guidée chimiquement |
| DE202016008460U1 (de) | 2015-03-06 | 2018-01-22 | Micromass Uk Limited | Zellpopulationsanalyse |
| CN112557491B (zh) * | 2015-03-06 | 2024-06-28 | 英国质谱公司 | 光谱分析 |
| CA2978165A1 (fr) | 2015-03-06 | 2016-09-15 | Micromass Uk Limited | Ionisation amelioree d'echantillons gazeux |
| EP3265822B1 (fr) | 2015-03-06 | 2021-04-28 | Micromass UK Limited | Analyse tissulaire par spectrométrie de masse ou par spectrométrie de mobilité ionique |
| US11282688B2 (en) | 2015-03-06 | 2022-03-22 | Micromass Uk Limited | Spectrometric analysis of microbes |
| CN107580675B (zh) | 2015-03-06 | 2020-12-08 | 英国质谱公司 | 拭子和活检样品的快速蒸发电离质谱（“reims”）和解吸电喷雾电离质谱（“desi-ms”）分析 |
| CA2977900A1 (fr) | 2015-03-06 | 2016-09-15 | Micromass Uk Limited | Surface de collision pour ionisation amelioree |
| GB2554181B (en) | 2015-03-06 | 2021-09-08 | Micromass Ltd | Inlet instrumentation for ion analyser coupled to rapid evaporative ionisation mass spectrometry ("REIMS") device |
| WO2016198984A1 (fr) * | 2015-06-11 | 2016-12-15 | Dh Technologies Development Pte. Ltd. | Procédé de déconvolution |
| GB201517195D0 (en) | 2015-09-29 | 2015-11-11 | Micromass Ltd | Capacitively coupled reims technique and optically transparent counter electrode |
| WO2017178833A1 (fr) | 2016-04-14 | 2017-10-19 | Micromass Uk Limited | Analyse spectrométrique de plantes |
| JP7334549B2 (ja) * | 2019-09-06 | 2023-08-29 | 株式会社島津製作所 | 微生物判別方法、微生物判別システム、及び微生物判別プログラム |
| CN114184668B (zh) * | 2020-09-15 | 2024-03-26 | 广州禾信康源医疗科技有限公司 | 微生物鉴定方法和双极性标准谱图生成方法 |
| CN113848261A (zh) * | 2021-08-24 | 2021-12-28 | 中南民族大学 | 一种基于脂质组学的真菌的鉴别方法 |
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| AU2001264867A1 (en) * | 2000-05-31 | 2001-12-11 | The Johns Hopkins University | Methods for using mass spectrometry to identify and classify filamentous fungi, yeasts, molds and pollen |
| US20040234952A1 (en) | 2003-01-07 | 2004-11-25 | Wibke Kallow | Process for identifying microoraganisms by means of mass spectrometry |
| WO2005085838A2 (fr) * | 2004-03-02 | 2005-09-15 | Vanderbilt University | Analyse computationnelle de matrices en lipidomique a spectroscopie de masse |
| FR2942806B1 (fr) | 2009-03-03 | 2011-09-02 | Assist Publ Hopitaux De Paris | Procede d'identification de germes en milieu liquide |
| FR2967691A1 (fr) * | 2010-11-24 | 2012-05-25 | Assist Publ Hopitaux Marseille | Procede d'identification d'un champignon, notamment d'un champignon filamenteux, et utilisations |
| US9273339B2 (en) | 2011-01-03 | 2016-03-01 | University Of Maryland, Baltimore | Methods for identifying bacteria |
| US8980577B2 (en) * | 2012-05-18 | 2015-03-17 | Bruker Daltonik Gmbh | Mass spectrometric identification of hyphal fungi |
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- 2014-10-09 CA CA2926427A patent/CA2926427C/fr active Active
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| US10465223B2 (en) | 2019-11-05 |
| JP2016537964A (ja) | 2016-12-08 |
| EP3055421A1 (fr) | 2016-08-17 |
| US20160215322A1 (en) | 2016-07-28 |
| CA2926427A1 (fr) | 2015-04-16 |
| WO2015054468A1 (fr) | 2015-04-16 |
| AU2014331866A1 (en) | 2016-04-21 |
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