CA2782964C - Parallel excitation system capable of spatial encoding and method thereof - Google Patents
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- 238000000799 fluorescence microscopy Methods 0.000 claims description 9
- 238000010171 animal model Methods 0.000 claims description 7
- 239000003550 marker Substances 0.000 claims description 7
- 238000013508 migration Methods 0.000 claims description 5
- 230000005012 migration Effects 0.000 claims description 5
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- G01D—MEASURING NOT SPECIALLY ADAPTED FOR A SPECIFIC VARIABLE; ARRANGEMENTS FOR MEASURING TWO OR MORE VARIABLES NOT COVERED IN A SINGLE OTHER SUBCLASS; TARIFF METERING APPARATUS; MEASURING OR TESTING NOT OTHERWISE PROVIDED FOR
- G01D5/00—Mechanical means for transferring the output of a sensing member; Means for converting the output of a sensing member to another variable where the form or nature of the sensing member does not constrain the means for converting; Transducers not specially adapted for a specific variable
- G01D5/26—Mechanical means for transferring the output of a sensing member; Means for converting the output of a sensing member to another variable where the form or nature of the sensing member does not constrain the means for converting; Transducers not specially adapted for a specific variable characterised by optical transfer means, i.e. using infrared, visible, or ultraviolet light
- G01D5/32—Mechanical means for transferring the output of a sensing member; Means for converting the output of a sensing member to another variable where the form or nature of the sensing member does not constrain the means for converting; Transducers not specially adapted for a specific variable characterised by optical transfer means, i.e. using infrared, visible, or ultraviolet light with attenuation or whole or partial obturation of beams of light
- G01D5/34—Mechanical means for transferring the output of a sensing member; Means for converting the output of a sensing member to another variable where the form or nature of the sensing member does not constrain the means for converting; Transducers not specially adapted for a specific variable characterised by optical transfer means, i.e. using infrared, visible, or ultraviolet light with attenuation or whole or partial obturation of beams of light the beams of light being detected by photocells
- G01D5/347—Mechanical means for transferring the output of a sensing member; Means for converting the output of a sensing member to another variable where the form or nature of the sensing member does not constrain the means for converting; Transducers not specially adapted for a specific variable characterised by optical transfer means, i.e. using infrared, visible, or ultraviolet light with attenuation or whole or partial obturation of beams of light the beams of light being detected by photocells using displacement encoding scales
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Abstract
A parallel excitation system based on spatial encoding technique and a method thereof are provided. The system includes a parallel excitation array consisting of a plurality of single-point excitation light sources (11) and a spatial encoding control system (2). The spatial encoding control system (2) includes a microcontroller (21), a driving module (22), a switch control module (23), an output light power control module (24) and an excitation time control module (25). The switch control module (23) is used for the control of each single-point excitation light source (11) to be turned on or off. The output light power of each single-point excitation light source (11) is controlled by the output light power control module (24). The operating time of each single-point excitation light source (11) is controlled by the excitation time control module (25).
The working parameters, such as the on or off, the output light power and the operation time of the single-point excitation light sources, are determined by the switch control module (23), the output light power control module (24) and the excitation time control module (25). The operating status of each single-point excitation light source (11) in the parallel excitation array is set by the microcontroller (21) with the driving module (22) according to corresponding parameters, and therefore spatial encoding for the parallel excitation array is achieved with the spatial encoding control system (2). The invention has wide applications with the advantages of flexibility, high efficiency, convenience, and high spatial resolution.
The working parameters, such as the on or off, the output light power and the operation time of the single-point excitation light sources, are determined by the switch control module (23), the output light power control module (24) and the excitation time control module (25). The operating status of each single-point excitation light source (11) in the parallel excitation array is set by the microcontroller (21) with the driving module (22) according to corresponding parameters, and therefore spatial encoding for the parallel excitation array is achieved with the spatial encoding control system (2). The invention has wide applications with the advantages of flexibility, high efficiency, convenience, and high spatial resolution.
Description
PARALLEL EXCITATION SYSTEM CAPABLE OF SPATIAL
ENCODING AND METHOD THEREOF
Technical Field The present invention relates to the technique of optical molecular imaging, particularly to a parallel excitation system based on spatial encoding technique and a method thereof used in fluorescence imaging.
Background Art Fluorescence molecular imaging is a new molecular imaging technique which developed rapidly in recent years. It has broad application prospects in the fields of tumor detection, drug development, and disease diagnosis. In fluorescence imaging, an external light with appropriate wavelength is used to excite specific fluorophore labeled molecules or cells, followed almost immediately by release of fluorescence. After scattering and absorption in biological tissues, the fluorescence signals can be detected by specific devices at the surface of the imaged object. The internal distributions of fluorochromes or chromophores in tissues can be obtained based on the fluorescence measurements. Normal or abnormal biological processes can be visualized at molecular and cellular levels spatially and temporally, with the advantages of high sensitive, non-ionizing radiation, non-invasive, and low cost.
However, with the conventional single-point light source excitation mode, only a limited volume of the imaged object near the excitation light source can be excited and fluorescence signals only from a local region can be acquired in fluorescence imaging. Fluorescent markers distributed in other volumes far away from the excitation light source may not be excited or only poorly excited. As a result, the obtained fluorescence information is incomplete and incorrect. Although fluorescence information at different regions can be obtained by successively changing the position of the single-point excitation light source to collect multiple cycles of projections, the complexity of the imaging system and the data acquisition time will be increased. It is unacceptable in practical cases where fluorescent signals with different temporal and spatial distributions need to be observed, especially in the imaging of fast dynamic process.
Summary of the Invention For the above issues, the present invention provides a parallel excitation system based on spatial encoding technique used for fluorescence imaging and method thereof.
In order to achieve above objective, the present invention employs the following technical solution: a parallel excitation system based on spatial encoding technique is characterized in that: the system includes a parallel excitation array consisting of a plurality of single-point excitation
ENCODING AND METHOD THEREOF
Technical Field The present invention relates to the technique of optical molecular imaging, particularly to a parallel excitation system based on spatial encoding technique and a method thereof used in fluorescence imaging.
Background Art Fluorescence molecular imaging is a new molecular imaging technique which developed rapidly in recent years. It has broad application prospects in the fields of tumor detection, drug development, and disease diagnosis. In fluorescence imaging, an external light with appropriate wavelength is used to excite specific fluorophore labeled molecules or cells, followed almost immediately by release of fluorescence. After scattering and absorption in biological tissues, the fluorescence signals can be detected by specific devices at the surface of the imaged object. The internal distributions of fluorochromes or chromophores in tissues can be obtained based on the fluorescence measurements. Normal or abnormal biological processes can be visualized at molecular and cellular levels spatially and temporally, with the advantages of high sensitive, non-ionizing radiation, non-invasive, and low cost.
However, with the conventional single-point light source excitation mode, only a limited volume of the imaged object near the excitation light source can be excited and fluorescence signals only from a local region can be acquired in fluorescence imaging. Fluorescent markers distributed in other volumes far away from the excitation light source may not be excited or only poorly excited. As a result, the obtained fluorescence information is incomplete and incorrect. Although fluorescence information at different regions can be obtained by successively changing the position of the single-point excitation light source to collect multiple cycles of projections, the complexity of the imaging system and the data acquisition time will be increased. It is unacceptable in practical cases where fluorescent signals with different temporal and spatial distributions need to be observed, especially in the imaging of fast dynamic process.
Summary of the Invention For the above issues, the present invention provides a parallel excitation system based on spatial encoding technique used for fluorescence imaging and method thereof.
In order to achieve above objective, the present invention employs the following technical solution: a parallel excitation system based on spatial encoding technique is characterized in that: the system includes a parallel excitation array consisting of a plurality of single-point excitation
2 light sources and a spatial encoding control system; the spatial encoding control system includes a microcontroller, a driving module, a switch control module, an output light power control module and an excitation time control module; wherein the switch control module controls each single-point excitation light source to be turned on or off, the output light power control module controls the output light power of each single-point excitation light source; the excitation time control module controls the operating time of each single-point excitation light source. The working parameters, such as the on or off, the output light power and the operation time of the single-point excitation light sources, are determined by the switch control module, the output light power control module and the excitation time control module. The working status of each single-point excitation light source in the parallel excitation array is controlled by the driver module. The microcontroller sends appropriate parameters that have been determined before to the driver module, and therefore achieves space encoding of the parallel excitation array.
The single-point excitation light source is a high power LED or a laser diode.
The parallel excitation array may be an array in a rectangular, round, fan shape or other shapes.
A method of parallel excitation with spatial encoding technique based on above system, includes the steps of: (1) determining a parallel
The single-point excitation light source is a high power LED or a laser diode.
The parallel excitation array may be an array in a rectangular, round, fan shape or other shapes.
A method of parallel excitation with spatial encoding technique based on above system, includes the steps of: (1) determining a parallel
3 excitation mode according to specific experiment conditions such as goals, objects and fluorescent markers of an experiment; (2) setting the determined parallel excitation mode into the spatial encoding control system. Spatial encoding of the parallel excitation array is achieved by controlling each single point excitation light source with the modules in the spatial encoding control system; (3) performing fluorescence imaging with the spatially encoded parallel excitation array.
The contents of the parallel excitation mode include the number and distribution of the single-point excitation light source, as well as the output light power, operating time and excitation sequence of each single-point excitation light source. It specifically includes the following four modes:
0 when the whole-body distribution of fluorescent markers needs to be imaged, a plurality of single-point excitation light sources are selected and set to work simultaneously to perform excitation, wherein the number of the single-point excitation light source is determined by the volume of the object, and the output light power and operating time of each single-point excitation light source are determined by specific experiment requirements;
0 when the distribution of fluorescent markers in a local region of the experimental animal needs to be imaged, a single-point excitation light source is selected to perform excitation according to where the region locates, wherein the output light power and operating time of the single-
The contents of the parallel excitation mode include the number and distribution of the single-point excitation light source, as well as the output light power, operating time and excitation sequence of each single-point excitation light source. It specifically includes the following four modes:
0 when the whole-body distribution of fluorescent markers needs to be imaged, a plurality of single-point excitation light sources are selected and set to work simultaneously to perform excitation, wherein the number of the single-point excitation light source is determined by the volume of the object, and the output light power and operating time of each single-point excitation light source are determined by specific experiment requirements;
0 when the distribution of fluorescent markers in a local region of the experimental animal needs to be imaged, a single-point excitation light source is selected to perform excitation according to where the region locates, wherein the output light power and operating time of the single-
4 point excitation light source are determined by specific experiment requirements; when a migration process of the fluorescent marker in the experimental animal needs to be tracked dynamically, a plurality of single-point excitation light sources are selected and set to work sequentially to perform excitation, with each single-point excitation light source being set to be turned on when the fluorescent marker passing it, wherein the output light power is determined by specific experiment requirements; ,CD other parallel excitation modes such as point-by-point scanning excitation, row-by-row(column-by-column) excitation, interlacing excitation in rows/columns, or alternate excitation in two rows/columns) can be selected according to practical needs.
Since the present invention employs the above technical solution, it has following advantages compared to the prior art: the present invention employs the spatial encoding based parallel excitation technique, which can control the operation status of each single-point excitation light source in the light source array according to practical needs. This technique can be used to observe the temporal and spatial distribution of fluorescent markers in the whole animal, to feature the distribution of fluorescent markers in local regions specifically, and to track a migration process of a certain fluorescent marker dynamically. Compared to the existing excitation mode with a single-point light source, the method of parallel excitation based on spatial encoding designed by the invention has wider applications with the advantages of flexibility, high efficiency, convenience, short imaging time, and high spatial resolution.
Brief Description of Figures FIG. 1 shows a block diagram of the structure of the present invention;
FIG. 2 shows a schematic view of the structure of a parallel excitation array in the present invention.
DETAILED DESCRIPTION
The present invention will be described in detail with reference to the embodiments of the present invention in conjunction with the accompanying drawings.
As shown in FIG. 1, the system of the present invention includes a parallel excitation array 1 and a spatial encoding control system 2.
As shown in FIG. 2, the parallel excitation array 1 includes a plurality of single-point excitation light source 11 (labeled as Si, in FIG.2, wherein i indicates the row where the single-point excitation light source 11 locates, and j indicates the column where the single-point excitation light sources 11 locates). A plurality of single-point excitation light sources 11 are arranged in a mxn array. in indicates the number of rows of the array, and n indicates the number of columns of the array, with a spacing of d1 between neighboring columns , and a spacing of d, between neighboring rows. m, n, d1, and d2 are determined according to practical needs.
As shown in FIG. 1, the spatial encoding control system 2 includes a microcontroller 21, a driving module 22, a switch control module 23, an output light power control module 24, and an excitation time control module 25. Wherein, the switch control module 23 controls each single-point excitation light source 11 to turn on or off; the output light power control module 24 controls the output light power of each single-point excitation light source 11; the excitation time control module 25 controls the operating time of each single-point excitation light source 11. The working parameters, such as the on or off, the output light power and the operation time of the single-point excitation light sources, are determined by the switch control module 23, the output light power control module 24 and the excitation time control module 25. The working status of each single-point light source 11 in the parallel excitation array 1 is controlled by the driver module 22. The microcontroller 21 sends appropriate parameters that have been determined before to the driver module 22, and therefore achieves space encoding of the parallel excitation array 1.
In the above embodiment, the single-point excitation light source 11 can be a high power LED (light emitting diode) or a laser diode.
In the above embodiment, the plurality of single-point excitation light source 11 may be arranged in an array of rectangular, round, fan or other shapes.
The use of the parallel excitation system based on spatial coding technique in fluorescence imaging includes the following steps:
(1) Determining a parallel excitation mode according to specific experiment conditions such as goals, objects and fluorescent markers of an experiment. The contents of the parallel excitation mode include the number and distribution of the single-point excitation light sources 11, as well as the output light power, operating time and excitation sequence of each single-point excitation light source 11. It specifically includes the following four modes:
When the whole-body distribution of fluorescent markers of an experimental animal needs to be imaged, i x j single-point excitation light sources 11 (Sõ S1, S¨ ) are selected and set to work simultaneously to perform excitation, wherein the values of i and j are determined by the volume of the object, and the output light power and operating time of each single-point excitation light source 11 are determined by specific experiment requirements;
When the distribution of fluorescent markers in a local region of the experimental animal needs to be imaged, a single-point excitation light sources S, is selected to perform excitation, wherein the values of i and j are determined by the position of the region, and the output light power and operating time of the single-point excitation light source 11 are determined by specific experiment requirements;
0 When a migration process of the fluorescent marker in the experimental animal needs to be tracked dynamically (assuming that a migration path is SH -4 Si 2 - -> S22 ---> S32 Si ¨> S34), a series of point sõ 512 Sõ S32 S33 Sm are selected and set to work sequentially to perform excitation, with each single-point excitation light source 11 being set to be turned on when the fluorescent marker passing it, wherein the output light power is determined by specific experiment requirements;
0 Other parallel excitation modes such as point-by-point scanning excitation, row-by-row (column-by-column) excitation, interlacing excitation in rows/columns, or alternate excitation in two rows/columns, may be selected according to practical needs.
(2) Setting the determined parallel excitation mode into the spatial coding control system 2. The operating state of each single-point excitation light source 11 (including whether the single-point excitation light sources 11 is turned on, its output light power and operating time, as well as the excitation order of the single-point excitation light sources 11) are set by the modules in the spatial encoding control system 2 to achieve spatial encoding of the parallel excitation array 1.
(3) Performing fluorescence imaging under the excitation of the spatially encoded parallel excitation array 1.
(4) When the imaging process ends, new fluorescence imaging experiment can be performed by adjusting the working mode of the parallel excitation array 1 with the spatial encoding control system 2 according to new experiment requirements.
While the present invention has been described with reference to the above embodiment, the structure, position and connection of each part can be changed. Based on the technical solution of the present invention, any modifications and equivalent changes to a specific part according to the principle of the present invention should not be excluded from the claimed scope of the invention.
Since the present invention employs the above technical solution, it has following advantages compared to the prior art: the present invention employs the spatial encoding based parallel excitation technique, which can control the operation status of each single-point excitation light source in the light source array according to practical needs. This technique can be used to observe the temporal and spatial distribution of fluorescent markers in the whole animal, to feature the distribution of fluorescent markers in local regions specifically, and to track a migration process of a certain fluorescent marker dynamically. Compared to the existing excitation mode with a single-point light source, the method of parallel excitation based on spatial encoding designed by the invention has wider applications with the advantages of flexibility, high efficiency, convenience, short imaging time, and high spatial resolution.
Brief Description of Figures FIG. 1 shows a block diagram of the structure of the present invention;
FIG. 2 shows a schematic view of the structure of a parallel excitation array in the present invention.
DETAILED DESCRIPTION
The present invention will be described in detail with reference to the embodiments of the present invention in conjunction with the accompanying drawings.
As shown in FIG. 1, the system of the present invention includes a parallel excitation array 1 and a spatial encoding control system 2.
As shown in FIG. 2, the parallel excitation array 1 includes a plurality of single-point excitation light source 11 (labeled as Si, in FIG.2, wherein i indicates the row where the single-point excitation light source 11 locates, and j indicates the column where the single-point excitation light sources 11 locates). A plurality of single-point excitation light sources 11 are arranged in a mxn array. in indicates the number of rows of the array, and n indicates the number of columns of the array, with a spacing of d1 between neighboring columns , and a spacing of d, between neighboring rows. m, n, d1, and d2 are determined according to practical needs.
As shown in FIG. 1, the spatial encoding control system 2 includes a microcontroller 21, a driving module 22, a switch control module 23, an output light power control module 24, and an excitation time control module 25. Wherein, the switch control module 23 controls each single-point excitation light source 11 to turn on or off; the output light power control module 24 controls the output light power of each single-point excitation light source 11; the excitation time control module 25 controls the operating time of each single-point excitation light source 11. The working parameters, such as the on or off, the output light power and the operation time of the single-point excitation light sources, are determined by the switch control module 23, the output light power control module 24 and the excitation time control module 25. The working status of each single-point light source 11 in the parallel excitation array 1 is controlled by the driver module 22. The microcontroller 21 sends appropriate parameters that have been determined before to the driver module 22, and therefore achieves space encoding of the parallel excitation array 1.
In the above embodiment, the single-point excitation light source 11 can be a high power LED (light emitting diode) or a laser diode.
In the above embodiment, the plurality of single-point excitation light source 11 may be arranged in an array of rectangular, round, fan or other shapes.
The use of the parallel excitation system based on spatial coding technique in fluorescence imaging includes the following steps:
(1) Determining a parallel excitation mode according to specific experiment conditions such as goals, objects and fluorescent markers of an experiment. The contents of the parallel excitation mode include the number and distribution of the single-point excitation light sources 11, as well as the output light power, operating time and excitation sequence of each single-point excitation light source 11. It specifically includes the following four modes:
When the whole-body distribution of fluorescent markers of an experimental animal needs to be imaged, i x j single-point excitation light sources 11 (Sõ S1, S¨ ) are selected and set to work simultaneously to perform excitation, wherein the values of i and j are determined by the volume of the object, and the output light power and operating time of each single-point excitation light source 11 are determined by specific experiment requirements;
When the distribution of fluorescent markers in a local region of the experimental animal needs to be imaged, a single-point excitation light sources S, is selected to perform excitation, wherein the values of i and j are determined by the position of the region, and the output light power and operating time of the single-point excitation light source 11 are determined by specific experiment requirements;
0 When a migration process of the fluorescent marker in the experimental animal needs to be tracked dynamically (assuming that a migration path is SH -4 Si 2 - -> S22 ---> S32 Si ¨> S34), a series of point sõ 512 Sõ S32 S33 Sm are selected and set to work sequentially to perform excitation, with each single-point excitation light source 11 being set to be turned on when the fluorescent marker passing it, wherein the output light power is determined by specific experiment requirements;
0 Other parallel excitation modes such as point-by-point scanning excitation, row-by-row (column-by-column) excitation, interlacing excitation in rows/columns, or alternate excitation in two rows/columns, may be selected according to practical needs.
(2) Setting the determined parallel excitation mode into the spatial coding control system 2. The operating state of each single-point excitation light source 11 (including whether the single-point excitation light sources 11 is turned on, its output light power and operating time, as well as the excitation order of the single-point excitation light sources 11) are set by the modules in the spatial encoding control system 2 to achieve spatial encoding of the parallel excitation array 1.
(3) Performing fluorescence imaging under the excitation of the spatially encoded parallel excitation array 1.
(4) When the imaging process ends, new fluorescence imaging experiment can be performed by adjusting the working mode of the parallel excitation array 1 with the spatial encoding control system 2 according to new experiment requirements.
While the present invention has been described with reference to the above embodiment, the structure, position and connection of each part can be changed. Based on the technical solution of the present invention, any modifications and equivalent changes to a specific part according to the principle of the present invention should not be excluded from the claimed scope of the invention.
Claims (5)
1. A parallel excitation system based on spatial encoding technique, characterized in that the system includes a parallel excitation array consisting of a plurality of single-point excitation light sources and a spatial encoding control system including a microcontroller, a driving module, a switch control module, an output light power control module and an excitation time control module; wherein the switch control module controls each single-point excitation light source to turn on or off; the output light power control module controls the output light power of each single-point excitation light source; the excitation time control module controls the operating time of each single-point excitation light source, wherein working parameters, such as the on or off, the output light power and the operation time of the single-point excitation light sources, are determined by the switch control module, the output light power control module and the excitation time control module; a working status of each single-point excitation light source in the parallel excitation array is controlled by the driver module, wherein the microcontroller sends the appropriate parameters that have been set before to the driver module, and therefore achieves space coding of the parallel excitation array.
2. The parallel excitation system based on spatial encoding technique according to claim 1, characterized in that the single-point excitation light source is a high power LED or a laser diode.
3. The parallel excitation system based on spatial encoding technique according to claim 1 or 2, characterized in that the parallel excitation array is an array of rectangular, round, fan or other shapes.
4. A method of parallel excitation with spatial encoding based on the system according to any of claims 1 to 3, including the steps of:
(1) Determining a parallel excitation mode according to specific experiment conditions such as goals, objects and fluorescent markers of an experiment;
(2) Setting the contents of the parallel excitation mode into the spatial coding control system after the mode determined according to specific experiment requirements, and then operating states of each single-point excitation light source are set by these modules in the spatial coding control system to achieve spatial encoding for the parallel excitation array;
(3) Performing fluorescence imaging under the excitation of the spatially encoded parallel excitation array.
(1) Determining a parallel excitation mode according to specific experiment conditions such as goals, objects and fluorescent markers of an experiment;
(2) Setting the contents of the parallel excitation mode into the spatial coding control system after the mode determined according to specific experiment requirements, and then operating states of each single-point excitation light source are set by these modules in the spatial coding control system to achieve spatial encoding for the parallel excitation array;
(3) Performing fluorescence imaging under the excitation of the spatially encoded parallel excitation array.
5. The method of parallel excitation based on spatial encoding technique according to claim 4, characterized in that the contents of the parallel excitation mode include the number and distribution of the single-point excitation light sources, as well as the output light power, operating time and excitation sequence of each single-point excitation light source, and the parallel excitation mode specifically includes the following four modes:
1 When the whole-body distribution of fluorescent markers needs to be imaged, a plurality of single-point excitation light sources are selected and set to work simultaneously to perform excitation, wherein the number of the single-point excitation light sources is determined by the volume of the object, and the output light power and operating time of each single-point excitation light source are determined by specific experimental requirements;
2 When the distribution of fluorescent markers in a local region of the experimental animal needs to be imaged, a single-point excitation light source is selected to perform excitation according to where the region locates, wherein the output light power and operating time of the single-point excitation light source are determined by specific experiment requirements;
3 When a migration process of the fluorescent marker in the experimental animal needs to be tracked dynamically, a plurality of single-point excitation light sources are selected and set to work sequentially to perform excitation, with each single-point excitation light source being set to be turned on when the fluorescent marker passing the single-point excitation light source, wherein the output light power is determined by specific experiment requirements;
4 Other parallel excitation modes such as point-by-point scanning excitation, row-by-row(column-by-column) sequential excitation, interlacing excitation in rows/columns, or alternate excitation in two rows/columns) can be selected according to practical needs.
1 When the whole-body distribution of fluorescent markers needs to be imaged, a plurality of single-point excitation light sources are selected and set to work simultaneously to perform excitation, wherein the number of the single-point excitation light sources is determined by the volume of the object, and the output light power and operating time of each single-point excitation light source are determined by specific experimental requirements;
2 When the distribution of fluorescent markers in a local region of the experimental animal needs to be imaged, a single-point excitation light source is selected to perform excitation according to where the region locates, wherein the output light power and operating time of the single-point excitation light source are determined by specific experiment requirements;
3 When a migration process of the fluorescent marker in the experimental animal needs to be tracked dynamically, a plurality of single-point excitation light sources are selected and set to work sequentially to perform excitation, with each single-point excitation light source being set to be turned on when the fluorescent marker passing the single-point excitation light source, wherein the output light power is determined by specific experiment requirements;
4 Other parallel excitation modes such as point-by-point scanning excitation, row-by-row(column-by-column) sequential excitation, interlacing excitation in rows/columns, or alternate excitation in two rows/columns) can be selected according to practical needs.
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| JP4087397B2 (en) * | 1996-03-19 | 2008-05-21 | 松下電器産業株式会社 | Fluorescence diagnostic treatment device |
| FR2842407B1 (en) * | 2002-07-18 | 2005-05-06 | Mauna Kea Technologies | "METHOD AND APPARATUS OF FIBROUS CONFOCAL FLUORESCENCE IMAGING" |
| DE102005013042A1 (en) * | 2005-03-18 | 2006-09-28 | Siemens Ag | Three-dimensional fluorescence/luminescence scanner for detection of e.g. breast cancer, computes three-dimensional position information of surface points of scanned body based on projection and recording angles of optical pattern |
| US8314406B2 (en) * | 2007-04-06 | 2012-11-20 | The General Hospital Corporation | Systems and methods for optical imaging using early arriving photons |
| EP2074933B1 (en) * | 2007-12-19 | 2012-05-02 | Kantonsspital Aarau AG | Method of analysing and processing fluorescent images |
| CN101539518B (en) * | 2008-03-20 | 2011-06-15 | 中国科学院自动化研究所 | Finite-element reconstruction method for space weighting of auto-fluorescence imaging |
| CN101396262B (en) * | 2008-10-31 | 2010-06-23 | 清华大学 | Fluorescent molecule tomography rebuilding method based on linear relationship |
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- 2010-03-02 CA CA2782964A patent/CA2782964C/en not_active Expired - Fee Related
- 2010-03-02 CN CN201080000866.2A patent/CN102036602B/en not_active Expired - Fee Related
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| CN102036602B (en) | 2012-09-05 |
| CA2782964A1 (en) | 2011-09-09 |
| WO2011106905A1 (en) | 2011-09-09 |
| CN102036602A (en) | 2011-04-27 |
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