CA2782176C - Procedes et appareil destines a la segregation de particules, y compris la segregation et la proliferation de cellules foetales et souches - Google Patents

Procedes et appareil destines a la segregation de particules, y compris la segregation et la proliferation de cellules foetales et souches Download PDF

Info

Publication number
CA2782176C
CA2782176C CA2782176A CA2782176A CA2782176C CA 2782176 C CA2782176 C CA 2782176C CA 2782176 A CA2782176 A CA 2782176A CA 2782176 A CA2782176 A CA 2782176A CA 2782176 C CA2782176 C CA 2782176C
Authority
CA
Canada
Prior art keywords
cells
passage
particles
fluid
cover
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Active
Application number
CA2782176A
Other languages
English (en)
Other versions
CA2782176A1 (fr
Inventor
George Hvichia
David Counts
Gary Evans
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Angle North America Inc
Original Assignee
Parsortix Inc
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Parsortix Inc filed Critical Parsortix Inc
Publication of CA2782176A1 publication Critical patent/CA2782176A1/fr
Application granted granted Critical
Publication of CA2782176C publication Critical patent/CA2782176C/fr
Active legal-status Critical Current
Anticipated expiration legal-status Critical

Links

Classifications

    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/5005—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving human or animal cells
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L3/00—Containers or dishes for laboratory use, e.g. laboratory glassware; Droppers
    • B01L3/50—Containers for the purpose of retaining a material to be analysed, e.g. test tubes
    • B01L3/502—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures
    • B01L3/5027—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures by integrated microfluidic structures, i.e. dimensions of channels and chambers are such that surface tension forces are important, e.g. lab-on-a-chip
    • B01L3/502753—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures by integrated microfluidic structures, i.e. dimensions of channels and chambers are such that surface tension forces are important, e.g. lab-on-a-chip characterised by bulk separation arrangements on lab-on-a-chip devices, e.g. for filtration or centrifugation
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/02—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving viable microorganisms
    • C12Q1/04—Determining presence or kind of microorganism; Use of selective media for testing antibiotics or bacteriocides; Compositions containing a chemical indicator therefor
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N1/00—Sampling; Preparing specimens for investigation
    • G01N1/28—Preparing specimens for investigation including physical details of (bio-)chemical methods covered elsewhere, e.g. G01N33/50, C12Q
    • G01N1/40—Concentrating samples
    • G01N1/4077—Concentrating samples by other techniques involving separation of suspended solids
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N15/00—Investigating characteristics of particles; Investigating permeability, pore-volume or surface-area of porous materials
    • G01N15/02—Investigating particle size or size distribution
    • G01N15/0272—Investigating particle size or size distribution with screening; with classification by filtering
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/483—Physical analysis of biological material
    • G01N33/487—Physical analysis of biological material of liquid biological material
    • G01N33/49—Blood
    • G01N33/491—Blood by separating the blood components
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
    • G01N33/543—Immunoassay; Biospecific binding assay; Materials therefor with an insoluble carrier for immobilising immunochemicals
    • G01N33/54366—Apparatus specially adapted for solid-phase testing
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2300/00—Additional constructional details
    • B01L2300/08—Geometry, shape and general structure
    • B01L2300/0809—Geometry, shape and general structure rectangular shaped
    • B01L2300/0816—Cards, e.g. flat sample carriers usually with flow in two horizontal directions
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2300/00—Additional constructional details
    • B01L2300/08—Geometry, shape and general structure
    • B01L2300/0861—Configuration of multiple channels and/or chambers in a single devices
    • B01L2300/087—Multiple sequential chambers
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2400/00—Moving or stopping fluids
    • B01L2400/08—Regulating or influencing the flow resistance
    • B01L2400/084—Passive control of flow resistance
    • B01L2400/086—Passive control of flow resistance using baffles or other fixed flow obstructions

Landscapes

  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Immunology (AREA)
  • Biomedical Technology (AREA)
  • Analytical Chemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Physics & Mathematics (AREA)
  • Molecular Biology (AREA)
  • Hematology (AREA)
  • Biochemistry (AREA)
  • Urology & Nephrology (AREA)
  • Pathology (AREA)
  • General Physics & Mathematics (AREA)
  • Medicinal Chemistry (AREA)
  • Organic Chemistry (AREA)
  • Biotechnology (AREA)
  • Microbiology (AREA)
  • Food Science & Technology (AREA)
  • Biophysics (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Cell Biology (AREA)
  • Dispersion Chemistry (AREA)
  • Tropical Medicine & Parasitology (AREA)
  • Toxicology (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Clinical Laboratory Science (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • Genetics & Genomics (AREA)
  • Ecology (AREA)
  • Apparatus Associated With Microorganisms And Enzymes (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Investigating Or Analysing Biological Materials (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)

Abstract

La présente invention concerne un appareil destiné à la ségrégation de particules en se basant sur leur capacité à circuler à travers une voie de passage étagée. Au moins une partie des particules sont disposées dans un passage lié à un premier étage, mais au moins une partie des particules ne sont pas capables de passer à travers un passage plus petit lié à un second étage, résultant en la ségrégation des particules. Les appareils et procédés décrits ici peuvent être utilisés dans la ségrégation de particules d'une grande variété de types. A titre d'exemple, ils peuvent être utilisés dans la ségrégation de cellules de type ftales provenant d'un échantillon de sang maternel tel que du sang artériel maternel.
CA2782176A 2009-11-30 2010-11-29 Procedes et appareil destines a la segregation de particules, y compris la segregation et la proliferation de cellules foetales et souches Active CA2782176C (fr)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
US26491809P 2009-11-30 2009-11-30
US61/264,918 2009-11-30
PCT/US2010/058172 WO2011066497A2 (fr) 2009-11-30 2010-11-29 Procédés et appareil destinés à la ségrégation de particules, y compris la ségrégation et la prolifération de cellules fœtales et souches

Publications (2)

Publication Number Publication Date
CA2782176A1 CA2782176A1 (fr) 2011-06-03
CA2782176C true CA2782176C (fr) 2015-10-06

Family

ID=43332885

Family Applications (1)

Application Number Title Priority Date Filing Date
CA2782176A Active CA2782176C (fr) 2009-11-30 2010-11-29 Procedes et appareil destines a la segregation de particules, y compris la segregation et la proliferation de cellules foetales et souches

Country Status (8)

Country Link
EP (1) EP2507382A4 (fr)
JP (2) JP2013511992A (fr)
CN (1) CN102695804B (fr)
AU (1) AU2010246381B2 (fr)
BR (1) BR112012013034A2 (fr)
CA (1) CA2782176C (fr)
MX (1) MX2012006244A (fr)
WO (1) WO2011066497A2 (fr)

Families Citing this family (11)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP2587248A1 (fr) * 2011-10-25 2013-05-01 Koninklijke Philips Electronics N.V. Filtrage de particules à partir de sang ou autres supports
US9631179B2 (en) 2013-03-15 2017-04-25 Angle North America, Inc. Methods for segregating particles using an apparatus with a size-discriminating separation element having an elongate leading edge
US10539554B2 (en) 2014-10-27 2020-01-21 Board Of Regents, The University Of Texas System High-throughput imaging platform
CN105176812B (zh) * 2015-07-28 2017-10-03 浙江奥瑞健生物技术有限公司 一种干细胞球切割收集装置及干细胞球的切割分离传代方法
JP6839173B2 (ja) * 2015-08-18 2021-03-03 ナショナル ヘルス リサーチ インスティテューツＮａｔｉｏｎａｌ Ｈｅａｌｔｈ Ｒｅｓｅａｒｃｈ Ｉｎｓｔｉｔｕｔｅｓ 流体力学シャトリングチップ機器、及び、分離した単一細胞を捕捉する方法
GB201704766D0 (en) * 2017-01-05 2017-05-10 Illumia Inc System and methods for selective effluent collection
EP3561038A1 (fr) * 2018-04-24 2019-10-30 Bayer AG Séparateur de cellules
US11389799B2 (en) * 2019-01-17 2022-07-19 The Regents Of The University Of Michigan Microfluidic device for size and deformability measurements and applications thereof
US20220146400A1 (en) * 2019-02-27 2022-05-12 Kyocera Corporation Particle separating and measuring device and particle separating and measuring apparatus
CN109810894B (zh) * 2019-03-01 2022-06-10 长春理工大学 一种基于光诱导介电泳的群细胞三维结构操纵与构建***及方法
JP7502740B2 (ja) * 2019-07-23 2024-06-19 国立大学法人佐賀大学 剪断処理による微細藻類の外被破壊法及び外被破壊システム

Family Cites Families (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US6008040A (en) * 1995-07-07 1999-12-28 Synosys, Inc. Procedures for efficient separation of cells, cellular materials and proteins
WO2003008931A2 (fr) * 2001-07-17 2003-01-30 Georgi Hvichia Microstructure pour separation, identification, triage et manipulation de particules et de cellules
US6783928B2 (en) * 2001-07-17 2004-08-31 Georgi Hvichia Microstructures for cell proliferation assays and semen analysis
EP2359689B1 (fr) * 2002-09-27 2015-08-26 The General Hospital Corporation Dispositif microfluidique pour la séparation de cellules et usage du dispositif
JP2007522913A (ja) * 2003-10-03 2007-08-16 フリーイェ・ユニヴェルシテイト・ブリュッセル 流体中に存在する粒子を粒度分離するための方法及び装置
CN102083997B (zh) * 2008-04-23 2016-05-25 角度北美公司 用于分离颗粒的方法和设备

Also Published As

Publication number Publication date
EP2507382A4 (fr) 2016-06-08
JP2016208978A (ja) 2016-12-15
AU2010246381B2 (en) 2013-08-15
CA2782176A1 (fr) 2011-06-03
CN102695804A (zh) 2012-09-26
BR112012013034A2 (pt) 2016-10-04
WO2011066497A2 (fr) 2011-06-03
CN102695804B (zh) 2014-09-03
JP2013511992A (ja) 2013-04-11
AU2010246381A1 (en) 2010-12-16
EP2507382A2 (fr) 2012-10-10
MX2012006244A (es) 2012-10-15
WO2011066497A3 (fr) 2011-11-17

Similar Documents

Publication Publication Date Title
US20180299425A1 (en) Methods and Apparatus for Segregation of Particles
CA2782176C (fr) Procedes et appareil destines a la segregation de particules, y compris la segregation et la proliferation de cellules foetales et souches
JP6782998B2 (ja) 装置
US9534200B2 (en) Microstructure for particle and cell SEparation, identification, sorting, and manipulation
RU2539989C2 (ru) Система и способ фильтрации частиц
JP2011163830A (ja) サイズ選択マイクロキャビティアレイを用いた循環腫瘍細胞の検出
US20150076049A1 (en) Microfilter and apparatus for separating a biological entity from a sample volume
WO2010080978A2 (fr) Appauvrissement préalable en leucocytes dans des échantillons de sang total avant capture des constituants du sang total
EP3816274A1 (fr) Système de capture de cellules rares, et son application
WO2011028483A2 (fr) Procédés et appareil pour la ségrégation de particules, comprenant des sources supplémentaires de collecte de prélèvements pour la séparation de cellules foetales nucléées
Turmezei et al. Low-cost microfilter for red blood cell membrane stiffness measurement using photosensitive BCB

Legal Events

Date Code Title Description
EEER Examination request