CA2782176C - Procedes et appareil destines a la segregation de particules, y compris la segregation et la proliferation de cellules foetales et souches - Google Patents
Procedes et appareil destines a la segregation de particules, y compris la segregation et la proliferation de cellules foetales et souches Download PDFInfo
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- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
- G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
- G01N33/5005—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving human or animal cells
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- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
- B01L3/00—Containers or dishes for laboratory use, e.g. laboratory glassware; Droppers
- B01L3/50—Containers for the purpose of retaining a material to be analysed, e.g. test tubes
- B01L3/502—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures
- B01L3/5027—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures by integrated microfluidic structures, i.e. dimensions of channels and chambers are such that surface tension forces are important, e.g. lab-on-a-chip
- B01L3/502753—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures by integrated microfluidic structures, i.e. dimensions of channels and chambers are such that surface tension forces are important, e.g. lab-on-a-chip characterised by bulk separation arrangements on lab-on-a-chip devices, e.g. for filtration or centrifugation
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/02—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving viable microorganisms
- C12Q1/04—Determining presence or kind of microorganism; Use of selective media for testing antibiotics or bacteriocides; Compositions containing a chemical indicator therefor
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N1/00—Sampling; Preparing specimens for investigation
- G01N1/28—Preparing specimens for investigation including physical details of (bio-)chemical methods covered elsewhere, e.g. G01N33/50, C12Q
- G01N1/40—Concentrating samples
- G01N1/4077—Concentrating samples by other techniques involving separation of suspended solids
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N15/00—Investigating characteristics of particles; Investigating permeability, pore-volume or surface-area of porous materials
- G01N15/02—Investigating particle size or size distribution
- G01N15/0272—Investigating particle size or size distribution with screening; with classification by filtering
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
- G01N33/483—Physical analysis of biological material
- G01N33/487—Physical analysis of biological material of liquid biological material
- G01N33/49—Blood
- G01N33/491—Blood by separating the blood components
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
- G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
- G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
- G01N33/543—Immunoassay; Biospecific binding assay; Materials therefor with an insoluble carrier for immobilising immunochemicals
- G01N33/54366—Apparatus specially adapted for solid-phase testing
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
- B01L2300/00—Additional constructional details
- B01L2300/08—Geometry, shape and general structure
- B01L2300/0809—Geometry, shape and general structure rectangular shaped
- B01L2300/0816—Cards, e.g. flat sample carriers usually with flow in two horizontal directions
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- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
- B01L2300/00—Additional constructional details
- B01L2300/08—Geometry, shape and general structure
- B01L2300/0861—Configuration of multiple channels and/or chambers in a single devices
- B01L2300/087—Multiple sequential chambers
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- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
- B01L2400/00—Moving or stopping fluids
- B01L2400/08—Regulating or influencing the flow resistance
- B01L2400/084—Passive control of flow resistance
- B01L2400/086—Passive control of flow resistance using baffles or other fixed flow obstructions
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- Health & Medical Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Engineering & Computer Science (AREA)
- Immunology (AREA)
- Biomedical Technology (AREA)
- Analytical Chemistry (AREA)
- General Health & Medical Sciences (AREA)
- Physics & Mathematics (AREA)
- Molecular Biology (AREA)
- Hematology (AREA)
- Biochemistry (AREA)
- Urology & Nephrology (AREA)
- Pathology (AREA)
- General Physics & Mathematics (AREA)
- Medicinal Chemistry (AREA)
- Organic Chemistry (AREA)
- Biotechnology (AREA)
- Microbiology (AREA)
- Food Science & Technology (AREA)
- Biophysics (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Zoology (AREA)
- Wood Science & Technology (AREA)
- Cell Biology (AREA)
- Dispersion Chemistry (AREA)
- Tropical Medicine & Parasitology (AREA)
- Toxicology (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Clinical Laboratory Science (AREA)
- Bioinformatics & Cheminformatics (AREA)
- General Engineering & Computer Science (AREA)
- Genetics & Genomics (AREA)
- Ecology (AREA)
- Apparatus Associated With Microorganisms And Enzymes (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
- Investigating Or Analysing Biological Materials (AREA)
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
Abstract
La présente invention concerne un appareil destiné à la ségrégation de particules en se basant sur leur capacité à circuler à travers une voie de passage étagée. Au moins une partie des particules sont disposées dans un passage lié à un premier étage, mais au moins une partie des particules ne sont pas capables de passer à travers un passage plus petit lié à un second étage, résultant en la ségrégation des particules. Les appareils et procédés décrits ici peuvent être utilisés dans la ségrégation de particules d'une grande variété de types. A titre d'exemple, ils peuvent être utilisés dans la ségrégation de cellules de type ftales provenant d'un échantillon de sang maternel tel que du sang artériel maternel.
Applications Claiming Priority (3)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
US26491809P | 2009-11-30 | 2009-11-30 | |
US61/264,918 | 2009-11-30 | ||
PCT/US2010/058172 WO2011066497A2 (fr) | 2009-11-30 | 2010-11-29 | Procédés et appareil destinés à la ségrégation de particules, y compris la ségrégation et la prolifération de cellules fœtales et souches |
Publications (2)
Publication Number | Publication Date |
---|---|
CA2782176A1 CA2782176A1 (fr) | 2011-06-03 |
CA2782176C true CA2782176C (fr) | 2015-10-06 |
Family
ID=43332885
Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
CA2782176A Active CA2782176C (fr) | 2009-11-30 | 2010-11-29 | Procedes et appareil destines a la segregation de particules, y compris la segregation et la proliferation de cellules foetales et souches |
Country Status (8)
Country | Link |
---|---|
EP (1) | EP2507382A4 (fr) |
JP (2) | JP2013511992A (fr) |
CN (1) | CN102695804B (fr) |
AU (1) | AU2010246381B2 (fr) |
BR (1) | BR112012013034A2 (fr) |
CA (1) | CA2782176C (fr) |
MX (1) | MX2012006244A (fr) |
WO (1) | WO2011066497A2 (fr) |
Families Citing this family (11)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
EP2587248A1 (fr) * | 2011-10-25 | 2013-05-01 | Koninklijke Philips Electronics N.V. | Filtrage de particules à partir de sang ou autres supports |
US9631179B2 (en) | 2013-03-15 | 2017-04-25 | Angle North America, Inc. | Methods for segregating particles using an apparatus with a size-discriminating separation element having an elongate leading edge |
US10539554B2 (en) | 2014-10-27 | 2020-01-21 | Board Of Regents, The University Of Texas System | High-throughput imaging platform |
CN105176812B (zh) * | 2015-07-28 | 2017-10-03 | 浙江奥瑞健生物技术有限公司 | 一种干细胞球切割收集装置及干细胞球的切割分离传代方法 |
JP6839173B2 (ja) * | 2015-08-18 | 2021-03-03 | ナショナル ヘルス リサーチ インスティテューツNational Health Research Institutes | 流体力学シャトリングチップ機器、及び、分離した単一細胞を捕捉する方法 |
GB201704766D0 (en) * | 2017-01-05 | 2017-05-10 | Illumia Inc | System and methods for selective effluent collection |
EP3561038A1 (fr) * | 2018-04-24 | 2019-10-30 | Bayer AG | Séparateur de cellules |
US11389799B2 (en) * | 2019-01-17 | 2022-07-19 | The Regents Of The University Of Michigan | Microfluidic device for size and deformability measurements and applications thereof |
US20220146400A1 (en) * | 2019-02-27 | 2022-05-12 | Kyocera Corporation | Particle separating and measuring device and particle separating and measuring apparatus |
CN109810894B (zh) * | 2019-03-01 | 2022-06-10 | 长春理工大学 | 一种基于光诱导介电泳的群细胞三维结构操纵与构建***及方法 |
JP7502740B2 (ja) * | 2019-07-23 | 2024-06-19 | 国立大学法人佐賀大学 | 剪断処理による微細藻類の外被破壊法及び外被破壊システム |
Family Cites Families (6)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
US6008040A (en) * | 1995-07-07 | 1999-12-28 | Synosys, Inc. | Procedures for efficient separation of cells, cellular materials and proteins |
WO2003008931A2 (fr) * | 2001-07-17 | 2003-01-30 | Georgi Hvichia | Microstructure pour separation, identification, triage et manipulation de particules et de cellules |
US6783928B2 (en) * | 2001-07-17 | 2004-08-31 | Georgi Hvichia | Microstructures for cell proliferation assays and semen analysis |
EP2359689B1 (fr) * | 2002-09-27 | 2015-08-26 | The General Hospital Corporation | Dispositif microfluidique pour la séparation de cellules et usage du dispositif |
JP2007522913A (ja) * | 2003-10-03 | 2007-08-16 | フリーイェ・ユニヴェルシテイト・ブリュッセル | 流体中に存在する粒子を粒度分離するための方法及び装置 |
CN102083997B (zh) * | 2008-04-23 | 2016-05-25 | 角度北美公司 | 用于分离颗粒的方法和设备 |
-
2010
- 2010-11-23 AU AU2010246381A patent/AU2010246381B2/en active Active
- 2010-11-29 BR BR112012013034A patent/BR112012013034A2/pt not_active IP Right Cessation
- 2010-11-29 CA CA2782176A patent/CA2782176C/fr active Active
- 2010-11-29 JP JP2012541219A patent/JP2013511992A/ja active Pending
- 2010-11-29 EP EP10834005.0A patent/EP2507382A4/fr not_active Withdrawn
- 2010-11-29 CN CN201080060399.2A patent/CN102695804B/zh active Active
- 2010-11-29 MX MX2012006244A patent/MX2012006244A/es not_active Application Discontinuation
- 2010-11-29 WO PCT/US2010/058172 patent/WO2011066497A2/fr active Application Filing
-
2016
- 2016-06-01 JP JP2016109647A patent/JP2016208978A/ja active Pending
Also Published As
Publication number | Publication date |
---|---|
EP2507382A4 (fr) | 2016-06-08 |
JP2016208978A (ja) | 2016-12-15 |
AU2010246381B2 (en) | 2013-08-15 |
CA2782176A1 (fr) | 2011-06-03 |
CN102695804A (zh) | 2012-09-26 |
BR112012013034A2 (pt) | 2016-10-04 |
WO2011066497A2 (fr) | 2011-06-03 |
CN102695804B (zh) | 2014-09-03 |
JP2013511992A (ja) | 2013-04-11 |
AU2010246381A1 (en) | 2010-12-16 |
EP2507382A2 (fr) | 2012-10-10 |
MX2012006244A (es) | 2012-10-15 |
WO2011066497A3 (fr) | 2011-11-17 |
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