CA2725573A1 - Novel 1,2,4 oxadiazole compounds and methods of use thereof - Google Patents

Novel 1,2,4 oxadiazole compounds and methods of use thereof Download PDF

Info

Publication number
CA2725573A1
CA2725573A1 CA2725573A CA2725573A CA2725573A1 CA 2725573 A1 CA2725573 A1 CA 2725573A1 CA 2725573 A CA2725573 A CA 2725573A CA 2725573 A CA2725573 A CA 2725573A CA 2725573 A1 CA2725573 A1 CA 2725573A1
Authority
CA
Canada
Prior art keywords
pyridin
oxadiazole
oxadiazol
compound
benzo
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Abandoned
Application number
CA2725573A
Other languages
French (fr)
Inventor
Jianguo Ji
Chih-Hung Lee
Kevin B. Sippy
Tao Li
Murali Gopalakrishnan
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Abbott Laboratories
Original Assignee
Abbott Laboratories
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Abbott Laboratories filed Critical Abbott Laboratories
Publication of CA2725573A1 publication Critical patent/CA2725573A1/en
Abandoned legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07D—HETEROCYCLIC COMPOUNDS
    • C07D271/00—Heterocyclic compounds containing five-membered rings having two nitrogen atoms and one oxygen atom as the only ring hetero atoms
    • C07D271/02—Heterocyclic compounds containing five-membered rings having two nitrogen atoms and one oxygen atom as the only ring hetero atoms not condensed with other rings
    • C07D271/06—1,2,4-Oxadiazoles; Hydrogenated 1,2,4-oxadiazoles
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K31/00—Medicinal preparations containing organic active ingredients
    • A61K31/33—Heterocyclic compounds
    • A61K31/395—Heterocyclic compounds having nitrogen as a ring hetero atom, e.g. guanethidine or rifamycins
    • A61K31/495—Heterocyclic compounds having nitrogen as a ring hetero atom, e.g. guanethidine or rifamycins having six-membered rings with two or more nitrogen atoms as the only ring heteroatoms, e.g. piperazine or tetrazines
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K45/00—Medicinal preparations containing active ingredients not provided for in groups A61K31/00 - A61K41/00
    • A61K45/06—Mixtures of active ingredients without chemical characterisation, e.g. antiphlogistics and cardiaca
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P21/00—Drugs for disorders of the muscular or neuromuscular system
    • A61P21/02—Muscle relaxants, e.g. for tetanus or cramps
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P25/00—Drugs for disorders of the nervous system
    • A61P25/04—Centrally acting analgesics, e.g. opioids
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P25/00—Drugs for disorders of the nervous system
    • A61P25/14—Drugs for disorders of the nervous system for treating abnormal movements, e.g. chorea, dyskinesia
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P25/00—Drugs for disorders of the nervous system
    • A61P25/18—Antipsychotics, i.e. neuroleptics; Drugs for mania or schizophrenia
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P25/00—Drugs for disorders of the nervous system
    • A61P25/24—Antidepressants
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P25/00—Drugs for disorders of the nervous system
    • A61P25/28—Drugs for disorders of the nervous system for treating neurodegenerative disorders of the central nervous system, e.g. nootropic agents, cognition enhancers, drugs for treating Alzheimer's disease or other forms of dementia
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P25/00—Drugs for disorders of the nervous system
    • A61P25/30—Drugs for disorders of the nervous system for treating abuse or dependence
    • A61P25/32—Alcohol-abuse
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P25/00—Drugs for disorders of the nervous system
    • A61P25/30—Drugs for disorders of the nervous system for treating abuse or dependence
    • A61P25/34—Tobacco-abuse
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P43/00—Drugs for specific purposes, not provided for in groups A61P1/00-A61P41/00
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07D—HETEROCYCLIC COMPOUNDS
    • C07D413/00—Heterocyclic compounds containing two or more hetero rings, at least one ring having nitrogen and oxygen atoms as the only ring hetero atoms
    • C07D413/02—Heterocyclic compounds containing two or more hetero rings, at least one ring having nitrogen and oxygen atoms as the only ring hetero atoms containing two hetero rings
    • C07D413/04—Heterocyclic compounds containing two or more hetero rings, at least one ring having nitrogen and oxygen atoms as the only ring hetero atoms containing two hetero rings directly linked by a ring-member-to-ring-member bond
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07D—HETEROCYCLIC COMPOUNDS
    • C07D413/00—Heterocyclic compounds containing two or more hetero rings, at least one ring having nitrogen and oxygen atoms as the only ring hetero atoms
    • C07D413/14—Heterocyclic compounds containing two or more hetero rings, at least one ring having nitrogen and oxygen atoms as the only ring hetero atoms containing three or more hetero rings
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07D—HETEROCYCLIC COMPOUNDS
    • C07D471/00—Heterocyclic compounds containing nitrogen atoms as the only ring hetero atoms in the condensed system, at least one ring being a six-membered ring with one nitrogen atom, not provided for by groups C07D451/00 - C07D463/00
    • C07D471/02—Heterocyclic compounds containing nitrogen atoms as the only ring hetero atoms in the condensed system, at least one ring being a six-membered ring with one nitrogen atom, not provided for by groups C07D451/00 - C07D463/00 in which the condensed system contains two hetero rings
    • C07D471/04—Ortho-condensed systems

Abstract

The invention relates to 1,2,4 oxadiazole compounds and analogs thereof, represented by formula (II), and compo-sitions and methods of use thereof

Description

2 PCT/US2008/066002 NOVEL 1,2, 4 OXADIAZOLE COMPOUNDS AND METHODS OF USE THEREOF
BACKGROUND OF THE INVENTION

Technical Field [0001] The invention relates to novel compounds and compositions comprising oxadiazole derivatives, and methods of using the same.

Description of Related Technology [0002] The endogenous cholinergic neurotransmitter, acetylcholine (ACh), exerts its biological effect via two types of cholinergic receptors, the muscarinic acetylcholine receptors (mAChR) and the nicotinic acetylcholine receptors (nAChR). nAChRs are pentameric assemblies of subunits surrounding a central pore that gates the flux of Na-'-, K+

and Ca 2-1- ions. At least 16 subunit proteins, i.e. a2-a10, (31-(310, y, 6 and E, have been identified in neuronal tissues. These subunits provide for a great variety of homomeric and heteromeric combinations that account for the diverse receptor subtypes. For example, functional neuronal nAChR or neuronal nicotinic receptor (NNR) assemblies can be homomeric, comprising a7 or a8 or a9 subunits, or heteromeric, usually with at least one subunit from the a group (a2, a3, a4, a6) and the remainder from the (3 group ((32, (34). In the central nervous system, a4(32-containing NNR and a7-containing NNR
subtypes are the most widespread and mediate synaptic and, possibly, paracrine functions. These NNRs are expressed at high levels in areas involved with learning and memory, and play key roles in modulating neurotransmission in these regions. Reduced cholinergic activity and dysregulation of NNRs have been correlated with disease states involving cognitive deficits, progressive dementia, and epilepsy. Accordingly, these NNRs are implicated in a range of physiological and patho-physiological functions related to cognitive function, learning and memory, reward, motor control, arousal and analgesia (reviewed in Gopalakrishnan, M. et al., Ion channels - Ligand-gated. Comprehensive Medicinal Chemistry II, Edited by Triggle D.
J. et al., Major Reference Works, Elsevier. Unit 2.22, pp 877-918, 2006).
[0003] Neuronal nicotinic receptors, especially a4(32 neuronal nicotinic acetylcholine receptors (nAChRs) have been targeted for pain and various central nervous system diseases.
Antisense knockdown of the a4 subunit was found to decrease the analgesic effect of agonists (Bitner RS, et al., Brain Res. 871:66-74, 2000). Reduced antinociceptive responses to nicotine also is seen in a4 gene knockout animals (Marubio LM, et al., Nature 398:805-810, 1999). Both a4 and (32 nAChRs are responsible for mediating nicotinic analgesia at supraspinal responses and spinal sites (Decker, MW, et al., Curr Top Med Chem., 4: 369-384, 2004). Antinociceptive effects through a4(32 nAChRs are generally attributed to stimulation of brainstem monoaminergic transmission, particularly in the raphe (Cucchiaro G, et al., J
Pharmacol Exp Ther. 313:389-394, 2005). However, a4(32 stimulation of GABAergic and glycinergic inhibitory transmission in the spinal cord also may contribute (Rashid MH, et al., Pain 125:125-135, 2006).
[0004] Central a3* nAChRs may contribute to nicotinic analgesia (Khan IM, et al., J
Neurocytol. 33:543-556, 2004), but a3134 ligands are of little interest because of likely autonomic side effects. Indeed, the goal has been to avoid a3 * neuronal nicotinic receptor (NNR), as the dose-limiting emetic liability of nonselective compounds has been attributed to activation of a3 containing nAChRs. a3* nAChRs are expressed in the enteric nervous system as well as in other components of the peripheral and central nervous systems. Area postrema and nucleus tractus solitarius are brainstem nuclei thought to be involved in nausea and emesis. a3* nAChRs in the dorsal motor nucleus of the vagus and in nucleus tractus solitarius have been implicated in gastric and blood pressure responses to nicotine injected locally (Ferreira M, et al J. Pharmacol. Exp. Ther., 294:230-238, 2000).
[0005] Compounds with varying degrees of selectivity for a4(32 nAChRs over other nicotinic subtypes (a3, a7, al-containing) have been discovered over the years. For example, ABT-594 (referred to as Compound A in this application) was efficacious across a number of rodent models of nociception including acute thermal, chemogenic, neuropathic, and visceral pain (Decker MW, et al., Expert Opinion on Investigational Drugs, 10:1819-1830, 2001).
Available data suggest that ligands with selectivity for the a4(32 nAChRs over a3134 efficacy is preferred for low adverse event profiles. In theory, the therapeutic index could be expanded by (a) reducing a3134 activity or (b) increasing a4(32 efficacy without increasing a3(34 activity. The latter may be achieved by an a4(32 selective positive allosteric modulator (PAM) either alone or in combination with exogenous a4(32 agonist. Positive allosteric modulators can potentiate effects by enhancing the efficacy and or potency of agonists.

Accordingly, an a4(32 selective positive allosteric modulator can selectively enhance effects at the preferred a4(32 nAChRs over other nAChR subtypes.
[0006] Initially known positive allosteric modulators of the a4(32 nAChRs have been nonselective and not very potent. For example, nefiracetam has been reported to potentiate a4(32 nAChR responses (Narahashi T, et al., Biol.Pharm.Bull., 27:1701-1706, 2004). More recently, subtype selective PAMs have been disclosed. Compounds like 3-(3-pyridin-3-yl-1,2,4-oxadiazol-5-yl) benzonitrile and others have been described with robust a4(32 PAM
effects with little modulatory activity at other subtypes such as a3134 (e.g., see WO
2006/114400, published November 2, 2006).
[0007] Pain is an unmet medical need and the methods and possibilities for treatments of such indications are insufficient. Although continued efforts are being made to treat pain using nAChR agonists, robust efficacy in pain may be limited by the range of side effects associated with their use, albeit to differing degrees. In light of the significance of chronic pain and the limitations in their treatment, it would be beneficial to identify new methods of treating such disorders, particularly in a manner that reduces adverse ganglionic effects such as at the gastrointestinal systems (e.g. emesis). It would be particularly beneficial to identify compounds and compositions that offer an opportunity to wide the therapeutic window of nicotinic (nAChR) agonists in pain. Enhanced efficacy with nAChR ligands for the treatment of other central nervous system diseases such as cognitive and attention deficits is also desirable.
SUMMARY OF THE INVENTION
[0008] The invention relates to oxadiazole compounds, compositions comprising such compounds, and methods of using such compounds and compositions.
[0009] In one aspect, the invention is directed to compounds of formula II
Ar2N -Ara O-N
II, wherein Ari and Ar 2 are independently optionally substituted aryl or heteroaryl, or a pharmaceutically acceptable salt thereof.
[0010] Another aspect of the invention relates to pharmaceutical compositions comprising compounds of the invention. Such compositions can be administered in accordance with a method of the invention, typically as part of a therapeutic regimen for treatment or prevention of conditions and disorders related to nAChR activity, and more particularly a4(32 nAChR
positive allosteric modulator activity.
[0011] Yet another aspect of the invention relates to a method of modulating a4(32 nAChR positive allosteric modulator activity. The method is useful for treating, preventing or both treating and preventing conditions and disorders related to a4(32 nAChR positive allosteric modulator activity, particularly in mammals.
[0012] One embodiment of this invention provides compositions that are useful for treatment of diseases or disorders related to the nicotinic acetylcholine receptor (nAChR) with enhanced efficacy and less side effects than nicotinic agents alone.
[0013] Another embodiment of the invention relates to methods and compositions wherein the efficacy of a nicotinic (nAChR) agent is enhanced by co-dosing a nicotinic ligand with a positive allosteric modulator (PAM) of nAChR subtype a4132. Another embodiment of the invention relates to methods and compositions for treatment of individuals with nAChR-mediated diseases or disorders, and particularly for pain or CNS disorders, which involves a combination of a nicotinic ligand with an a4(32 positive allosteric modulator.
[0014] In one embodiment, the invention relates to methods and compositions comprising (i) a nicotinic acetylcholine receptor ligand; and (ii) a nicotinic acetylcholine receptor subtype a4(32 selective positive allosteric modulator, in admixture with at least one pharmaceutically acceptable excipient.

BRIEF DESCRIPTION OF THE DRAWINGS
[0015] Figures IA and lB depict responses of a representative nicotinic acetylcholine receptor ligand, 5-[(2R)-azetidin-2-ylmethoxy]-2-chloropyridine (Compound A) in the absence and presence of a nicotinic acetylcholine receptor subtype a4(32 positive allosteric modulator, 3-(3-(pyridin-3-yl)-1,2,4-oxadiazol-5-yl)benzonitrile (PAM, Compound 1), at human a4(32 or a3134 nicotinic acetylcholine receptor subtypes expressed in HEK-293 cells.
The data demonstrate a leftward shift in potency (EC50 value) at a4(32, but not a3(34, nAChRs.
[0016] Figures 2A and 2B depict responses of another representative nicotinic acetylcholine receptor ligand, (3R)-l-pyridin-3-ylpyrrolidin-3-amine (Compound B), in the absence and presence of an a4(32 positive allosteric modulator, 3-(3-(pyridin-3-yl)-1,2,4-oxadiazol-5-yl)benzonitrile (PAM, Compound 1), at human a4(32 or a3134 nicotinic receptor subtypes expressed in HEK-293 cells. Again, the data demonstrate a leftward shift in potency (EC50 value) of the nAChR agonist at a4(32, but not a3134 nAChRs.
[0017] Figures 3A and 3B graphically represent the effect of a4(32 positive allosteric modulator in enhancing the effect of a nAChR partial agonist, such as 2-methyl-3-(2-(S)-pyrrolidinylmethoxy)pyridine (Compound C, also known as ABT-089; Reuter, L.E., et al., CNS Drug Rev., 10 (2), 167-182, 2004). Compound C alone does not evoke a calcium response, but when co-applied with the PAM, 3-(3-(pyridin-3-yl)-1,2,4-oxadiazol-5-yl)benzonitrile (Compound 1), evoked robust responses at a4(32 nAChRs (Figure 3A), but not at a3134 nAChRs (Figure 3B). Compound C is a representative of other nicotinic partial agonists.
[0018] Figures 4A and 4B graphically represent the effect of an a4(32 positive allosteric modulator in enhancing the effect of another nAChR partial agonist (1 S,5 S)-3-(3,6-diaza-bicyclo[3.2.0]hept-3-yl)-quinoline (Compound D; a4(32 [3H]cytisine K; = 6 nM)).

Compound D alone does not evoke a response, but when co-applied with the PAM, 3-(3-(pyridin-3-yl)-1,2,4-oxadiazol-5-yl)benzonitrile (Compound 1), evoked robust responses at a4(32 nAChRs (Figure 4A), but not at a3(34 nAChRs (Figure 4B). Compound D is a representative of other nicotinic partial agonists.
[0019] Figure 5 shows correlation of potencies for activation of a4132 nAChRs by various nicotinic acetycholine receptor ligands in the presence and absence of an a4(32 PAM, 3-(3-(pyridin-3-yl)-1,2,4-oxadiazol-5-yl)benzonitrile (Compound 1). In general, these nicotinic ligands are found to be more potent in activating a4(32 nAChRs in the presence of a4(32 PAM (Compound 1).
[0020] Figure 6A graphically represents the effect of an a4(32 PAM, 3-(3-(pyridin-3-yl)-1,2,4-oxadiazol-5-yl)benzonitrile (Compound 1), on enhancing the efficacy by 5-[(2R)-azetidin-2-ylmethoxy]-2-chloropyridine (Compound A) in reversing neuropathic pain.
[0021] Figure 6B graphically represents the dose dependent effect of an a4(32 PAM, 3-(3-(pyridin-3-yl)-1,2,4-oxadiazol-5-yl)benzonitrile (Compound 1), on enhancing the neuropathic pain efficacy of 5-[(2R)-azetidin-2-ylmethoxy]-2-chloropyridine (Compound A).
An ineffective dose of Compound A (1 nmol/kg) demonstrates effect when combined with various doses of a4132 PAM (Compound 1).
[0022] Figure 7A shows dose-dependent effects in neuropathic pain of 5-[(2R)-azetidin-2-ylmethoxy]-2-chloropyridine (Compound A) alone, a4(32 PAM, 3-(3-(pyridin-3-yl)-1,2,4-oxadiazol-5-yl)benzonitrile (Compound 1), alone and a combination of Compound 1 (3.5 moUkg) with various doses of Compound A. An a4(32 PAM (Compound 1) alone is ineffective. However, in the presence of Compound 1 the dose response curve of Compound A in the Chung model of neuropathic pain shifts to the left.
[0023] Figure 7B shows the effects on emesis in ferrets. The effects of 5-[(2R)-azetidin-2-ylmethoxy]-2-chloropyridine (Compound A) alone, a4(32 PAM, 3-(3-(pyridin-3-yl)-1,2,4-oxadiazol-5-yl)benzonitrile (Compound 1), alone and a combination of Compound 1 (3.5 moUkg) with various doses of Compound A are shown. An a4(32 PAM (Compound 1) alone does not cause emesis, and does not shift the dose response curve of Compound A in the ferret model of emesis.
[0024] Figure 8A and 8B show plasma level analysis in models of neuropathic pain and emesis. The efficacy of Compound A is shifted left-ward as shown in Figure 8A, but no shift in effects on emesis are shown in Figure 8B. The maximal efficacy of Compound A can be realized in neuropathic pain without incidence of emesis, in presence of a4(32 PAM, 3-(3-(pyridin-3-yl)-1,2,4-oxadiazol-5-yl)benzonitrile (Compound 1). The data demonstrates that the therapeutic window of a4(32 nAChR agonists is wider in the presence of a4(32 PAM.
[0025] Figure 9 shows the efficacy of a partial agonist, Compound D, in the presence and absence of a4(32 PAM, 3-(3-(pyridin-3-yl)-1,2,4-oxadiazol-5-yl)benzonitrile (Compound 1).
In the example illustrated, Compound D when administered alone is ineffective in relieving pain. When co-dosed with a4(32 PAM (Compound 1), Compound D demonstrates effect, and the data demonstrate that Compound D provides significant relief of neuropathic pain in rats.
[0026] Figure 10 is a graphical representation of specific binding to receptor sites in human brain membranes (fmoles per mg protein) as a function of the concentration of a radioligand [3H]-3-(5-(pyridin-3-yl)-1,2,4-oxadiazol-3-yl)benzonitrile ([3H]-POB, nM).

DETAILED DESCRIPTION OF THE INVENTION
Definition of Terms
[0027] For a variable that occurs more than one time in any substituent or in the compound of the invention or any other formulae herein, its definition at each occurrence is independent of its definition at every other occurrence. Combinations of substituents are permissible only if such combinations result in stable compounds.
[0028] As used throughout this specification and the appended claims, the designation CX
- Cy, wherein x and y are integers from 1 to 10 refer to a range of carbon atoms in the hydrocarbon portion of the group which it modifies, for example, the designation "Cl - C6 haloalkyl" refers to at least one halogen appended to the parent molecular moiety through an alkyl group having from 1 to 6 carbon atoms.
[0029] As used in the specification and the appended claims, unless specified to the contrary, the following terms have the meaning indicated:
[0030] The term "acyl hydrazide", as used herein, means a -C(O)NHNH2 group.
[0031] The term "alkenyl", as used herein, means a straight or branched chain hydrocarbon containing from 2 to 10 carbons and containing at least one carbon-carbon double bond formed by the removal of two hydrogens. Representative examples of alkenyl include, but are not limited to, ethenyl, 2-propenyl, 2-methyl-2-propenyl, 3-butenyl, 4-pentenyl, 5-hexenyl, 2-heptenyl, 2-methyl-l-heptenyl, and 3-decenyl.
[0032] The term "alkoxy", as used herein, means an alkyl group, as defined herein, appended to the parent molecular moiety through an oxygen atom. Representative examples of alkoxy include, but are not limited to, methoxy, ethoxy, propoxy, 2-propoxy, butoxy, tert-butoxy, pentyloxy, and hexyloxy.
[0033] The term "alkoxyalkoxy", as used herein, means an alkoxy group, as defined herein, appended to the parent molecular moiety through another alkoxy group, as defined herein. Representative examples of alkoxyalkoxy include, but are not limited to, tert-butoxymethoxy, 2-ethoxyethoxy, 2-methoxyethoxy, and methoxymethoxy.
[0034] The term "alkoxyalkoxyalkyl", as used herein, means an alkoxyalkoxy group, as defined herein, appended to the parent molecular moiety through an alkyl group, as defined herein. Representative examples of alkoxyalkoxyalkyl include, but are not limited to, tert-butoxymethoxymethyl, ethoxymethoxymethyl, (2-methoxyethoxy)methyl, and 2-(2-methoxyethoxy) ethyl.
[0035] The term "alkoxyalkyl", as used herein, means an alkoxy group, as defined herein, appended to the parent molecular moiety through an alkyl group, as defined herein.
Representative examples of alkoxyalkyl include, but are not limited to, tert-butoxymethyl, 2-ethoxyethyl, 2-methoxyethyl, and methoxymethyl.
[0036] The term "alkoxycarbonyl", as used herein, means an alkoxy group, as defined herein, appended to the parent molecular moiety through a carbonyl group, as defined herein.
Representative examples of alkoxycarbonyl include, but are not limited to, methoxycarbonyl, ethoxycarbonyl, and tert-butoxycarbonyl.
[0037] The term "alkoxycarbonylalkyl", as used herein, means an alkoxycarbonyl group, as defined herein, appended to the parent molecular moiety through an alkyl group, as defined herein. Representative examples of alkoxycarbonylalkyl include, but are not limited to, 3-methoxycarbonylpropyl, 4-ethoxycarbonylbutyl, and 2-tert-butoxycarbonylethyl.
[0038] The term "alkoxycarbonylamino", as used herein, means an alkoxycarbonyl group, as defined herein, appended to the partent molecular moiety through an amino group, as defined herein. Representative examples of alkoxycarbonyamino include, but are not limited to, t-butoxycarbonylamino and methoxycarbonylamino.
[0039] The term "alkoxycarbonylaminoalkyl", as used herein, means an alkoxycarbonylamino group, as defined herein, appended to the partent molecular moiety through an alkyl group, as defined herein. Representative examples of alkoxycarbonyaminoalkyl include, but are not limited to, t-butoxycarbonylaminomethyl and methoxycarbonylaminopropyl.
[0040] The term "alkoxysulfonyl", as used herein, means an alkoxy group, as defined herein, appended to the parent molecular moiety through a sulfonyl group, as defined herein.
Representative examples of alkoxysulfonyl include, but are not limited to, methoxysulfonyl, ethoxysulfonyl and propoxysulfonyl.
[0041] The term "alkyl", as used herein, means a straight or branched chain hydrocarbon containing from 1 to 10 carbon atoms. Representative examples of alkyl include, but are not limited to, methyl, ethyl, n-propyl, iso-propyl, n-butyl, sec-butyl, iso-butyl, tert-butyl, n-pentyl, isopentyl, neopentyl, n-hexyl, 3-methylhexyl, 2,2-dimethylpentyl, 2,3-dimethylpentyl, n-heptyl, n-octyl, n-nonyl, and n-decyl.
[0042] The term "alkylamino", as used herein, means an alkyl group, as defined herein, appended to the parent molecular moiety through an amino group, as defined herein.
Representative examples of alkylamino include, but are not limited to, methylamino, ethylamino, and t-butylamino.
[0043] The term "alkylcarbonyl", as used herein, means an alkyl group, as defined herein, appended to the parent molecular moiety through a carbonyl group, as defined herein.
Representative examples of alkylcarbonyl include, but are not limited to, acetyl, 1-oxopropyl, 2,2-dimethyl-l-oxopropyl, 1-oxobutyl, and 1-oxopentyl.
[0044] The term "alkylcarbonylalkyl", as used herein, means an alkylcarbonyl group, as defined herein, appended to the parent molecular moiety through an alkyl group, as defined herein. Representative examples of alkylcarbonylalkyl include, but are not limited to, 2-oxopropyl, 3,3-dimethyl-2-oxopropyl, 3-oxobutyl, and 3-oxopentyl.
[0045] The term "alkylcarbonyloxy", as used herein, means an alkylcarbonyl group, as defined herein, appended to the parent molecular moiety through an oxygen atom.

Representative examples of alkylcarbonyloxy include, but are not limited to, acetyloxy, ethylcarbonyloxy, and tert-butylcarbonyloxy.
[0046] The term "alkylcarbonyloxylalkyl", as used herein, means an alkylcarbonyloxy group, as defined herein, appended to the parent molecular moiety through an alkyl group.
[0047] The term "alkylene", as used herein, means a divalent group derived from a straight or branched chain hydrocarbon of from 1 to 10 carbon atoms.
Representative examples of alkylene include, but are not limited to, -CH2-, -CH(CH3)-, -C(CH3)2-, -CH2CH2-, -CH2CH2CH2-, -CH2CH2CH2CH2-, and -CH2CH(CH3)CH2-.
[0048] The term "alkylsulfinyl", as used herein, means an alkyl group, as defined herein, appended to the parent molecular moiety through a sulfinyl group, as defined herein.
Representative examples of alkylsulfinyl include, but are not limited to, methylsulfinyl and ethylsulfinyl.
[0049] The term "alkylsulfinylalkyl", as used herein, means an alkylsulfinyl group, as defined herein, appended to the parent molecular moiety through an alkyl group, as defined herein. Representative examples of alkylsulfinylalkyl include, but are not limited to, methylsulfinylmethyl and ethylsulfinylmethyl.
[0050] The term "alkylsulfonyl", as used herein, means an alkyl group, as defined herein, appended to the parent molecular moiety through a sulfonyl group, as defined herein.
Representative examples of alkylsulfonyl include, but are not limited to, methylsulfonyl and ethylsulfonyl.
[0051] The term "alkylsulfonylalkyl", as used herein, means an alkylsulfonyl group, as defined herein, appended to the parent molecular moiety through an alkyl group, as defined herein. Representative examples of alkylsulfonylalkyl include, but are not limited to, methylsulfonylmethyl and ethylsulfonylmethyl.
[0052] The term "alkylthio", as used herein, means an alkyl group, as defined herein, appended to the parent molecular moiety through a sulfur atom. Representative examples of alkylthio include, but are not limited, methylthio, ethylthio, tert-butylthio, and hexylthio.
[0053] The term "alkylthioalkyl", as used herein, means an alkylthio group, as defined herein, appended to the parent molecular moiety through an alkyl group, as defined herein.
Representative examples of alkylthioalkyl include, but are not limited, methylthiomethyl and 2-(ethylthio)ethyl.
[0054] The term "alkynyl", as used herein, means a straight or branched chain hydrocarbon group containing from 2 to 10 carbon atoms and containing at least one carbon-carbon triple bond. Representative examples of alkynyl include, but are not limited, to acetylenyl, 1-propynyl, 2-propynyl, 3-butynyl, 2-pentynyl, and 1-butynyl.
[0055] The term "amino", as used herein, means a -NH2 group.
[0056] The term "aminoalkyl", as used herein, means at least one amino group, as defined herein, is appended to the parent molecular moiety through an alkyl group, as defined herein.
Representative examples of hydroxyalkyl include, but are not limited to, aminomethyl, 2-aminoethyl, 3-aminopropyl, and 2-ethyl-4-aminoheptyl.
[0057] The term "amido", as used herein, means an amino (H2N-), alkylamino (alkylN(H)-), dialkylamino (alkyl2N-), arylamino (arylN(H)-), arylalkylamino (arylalkylN(H)-) or another substituted amine group appended to the parent molecular moiety through a carbonyl group, as defined herein. Representative examples of amido include, but are not limited to, aminocarbonyl, methylaminocarbonyl, dimethylaminocarbonyl, and ethylmethylaminocarbonyl.
[0058] The term "aryl," as used herein, means phenyl, a bicyclic aryl or a tricyclic aryl.
The bicyclic aryl is naphthyl, a phenyl fused to a cycloalkyl, or a phenyl fused to a cycloalkenyl. Representative examples of the bicyclic aryl include, but are not limited to, dihydroindenyl, indenyl, naphthyl, dihydronaphthalenyl, and tetrahydronaphthalenyl. The tricyclic aryl is anthracene or phenanthrene, or a bicyclic aryl fused to a cycloalkyl, or a bicyclic aryl fused to a cycloalkenyl, or a bicyclic aryl fused to a phenyl.
Representative examples of tricyclic aryl ring include, but are not limited to, azulenyl, dihydroanthracenyl, fluorenyl, and tetrahydrophenanthrenyl.
[0059] The aryl groups of this invention can be substituted with 0, 1, 2, 3, 4 or 5 substituents independently selected from alkenyl, alkoxy, alkoxyalkoxy, alkoxyalkoxyalkyl, alkoxyalkyl, alkoxycarbonyl, alkoxycarbonylalkyl, alkyl, alkylcarbonyl, alkylcarbonylalkyl, alkylcarbonyloxy, alkylcarbonyloxyalkyl, alkylsulfinyl, alkylsulfinylalkyl, alkylsulfonyl, alkylsulfonylalkyl, alkylthio, alkylthioalkyl, alkynyl, arylalkyl, arylalkoxy, aryloxy, carboxy, carboxyalkyl, cyano, cyanoalkyl, formyl, formylalkyl, halogen, haloalkyl, haloalkoxy, hydroxy, hydroxyalkyl, mercapto, nitro, -NZ'Z2, and (NZ3Z4)carbonyl.
[0060] The term "arylalkoxy", as used herein, means an aryl group, as defined herein, appended to the parent molecular moiety through an alkoxy group, as defined herein.
Representative examples of arylalkoxy include, but are not limited to, 2-phenylethoxy, 3-naphth-2-ylpropoxy, and 5-phenylpentyloxy.
[0061] The term "arylalkyl", as used herein, means an aryl group, as defined herein, appended to the parent molecular moiety through an alkyl group, as defined herein.

Representative examples of arylalkyl include, but are not limited to, benzyl, 2-phenylethyl, 3-phenylpropyl, and 2-naphth-2-ylethyl.
[0062] The term "aryloxy", as used herein, means an aryl group, as defined herein, appended to the parent molecular moiety through an oxygen atom. Representative examples of aryloxy include, but are not limited to, phenoxy, naphthyloxy, 3-bromophenoxy, 4-chlorophenoxy, 4-methylphenoxy, and 3,5-dimethoxyphenoxy.
[0063] The term "carbonyl", as used herein, means a -C(O)- group.
[0064] The term "carboxy", as used herein, means a -CO2H group.
[0065] The term "carboxyalkyl", as used herein, means a carboxy group, as defined herein, appended to the parent molecular moiety through an alkyl group, as defined herein.
Representative examples of carboxyalkyl include, but are not limited to, carboxymethyl, 2-carboxyethyl, and 3-carboxypropyl.
[0066] The terms "comprise", "comprises" and "comprising", as used herein, are transitional terms, which are synonymous with "including," "containing," or "characterized by," are inclusive or open-ended and do not exclude additional, unrecited elements or method steps.
[0067] The term "concurrent administration" refers to administering the a4(32 receptor ligand to a patient, who has been prescribed (or has consumed) at least one an a4(32 PAM, at an appropriate time so that the patient's symptoms may subside. This may mean simultaneous administration of an a4(32 PAM and an a4(32 receptor ligand, or administration of the medications at different, but appropriate times. Establishing such a proper dosing schedule will be readily apparent to one skilled in the art, such as a physician treating various pain states.
[0068] The term "cyan", as used herein, means a -CN group.
[0069] The term "cyanoalkyl", as used herein, means a cyano group, as defined herein, appended to the parent molecular moiety through an alkyl group, as defined herein.
Representative examples of cyanoalkyl include, but are not limited to, cyanomethyl, 2-cyanoethyl, and 3-cyanopropyl.
[0070] The term "cycloalkenyl", as used herein, means a cyclic hydrocarbon containing from 3 to 8 carbons and containing at least one carbon-carbon double bond formed by the removal of two hydrogens. Representative examples of cycloalkenyl include, but are not limited to, 2-cyclohexen-l-yl, 3-cyclohexen-l-yl, 2,4-cyclohexadien-l-yl and 3-cyclopenten-1-yl.
[0071] The term "cycloalkyl", as used herein, means a monocyclic, bicyclic, or tricyclic ring system. Monocyclic ring systems are exemplified by a saturated cyclic hydrocarbon group containing from 3 to 8 carbon atoms. Examples of monocyclic ring systems include cyclopropyl, cyclobutyl, cyclopentyl, cyclohexyl, cycloheptyl, and cyclooctyl.
Bicyclic ring systems are exemplified by a bridged monocyclic ring system in which two adjacent or non-adjacent carbon atoms of the monocyclic ring are linked by an alkylene bridge of between one and three additional carbon atoms. Representative examples of bicyclic ring systems include, but are not limited to, bicyclo[3.1.1]heptane, bicyclo[2.2.1]heptane, bicyclo[2.2.2]octane, bicyclo[3.2.2]nonane, bicyclo[3.3.1]nonane, andbicyclo[4.2.1]nonane.
Tricyclic ring systems are exemplified by a bicyclic ring system in which two non-adjacent carbon atoms of the bicyclic ring are linked by a bond or an alkylene bridge of between one and three carbon atoms. Representative examples of tricyclic-ring systems include, but are not limited to, tricyclo[3.3.1.03'7]nonane and tricyclo[3.3.1.13'7]decane (adamantane).
[0072] The cycloalkyl groups of the invention are optionally substituted with 1, 2, 3, 4 or 5 substituents selected from the group consisting of alkenyl, alkoxy, alkoxyalkoxy, alkoxyalkyl, alkoxycarbonyl, alkoxysulfonyl, alkyl, alkylcarbonyl, alkylcarbonyloxy, alkylsulfonyl, alkylthio, alkylthioalkyl, alkynyl, carboxy, cyano, formyl, haloalkoxy, haloalkyl, halogen, hydroxy, hydroxyalkyl, mercapto, oxo, -NZ'Z2, and (NZ3Z4)carbonyl.
[0073] The term "cycloalkylalkyl", as used herein, means a cycloalkyl group, as defined herein, appended to the parent molecular moiety through an alkyl group, as defined herein.
Representative examples of cycloalkylalkyl include, but are not limited to, cyclopropylmethyl, 2-cyclobutylethyl, cyclopentylmethyl, cyclohexylmethyl, and cycloheptylbutyl.
[0074] The term "dialkylamino," as used herein, refers to two independent alkyl groups, as defined herein, appended to the parent molecular moiety through an amino group, as defined herein. Representative examples of dialkylamino include, but are not limited to, dimethylamino, diethylamino, ethylmethylamino, butylmethylamino, ethylhexylamino, and the like.
[0075] The term "dialkylaminoalkyl", as used herein, means a dialkyl group, as defined herein, appended to the parent molecular moiety through an alkyl group, as defined herein.
Representative examples of dialkylaminoalkyl include, but are not limited to, dimethylaminomethyl and dimethylaminoethyl.
[0076] The term "dialkylsulfonylformimidamide" as used herein, means a -SO2N=CH-N(alkyl)2 group.
[0077] The term "formyl", as used herein, means a -C(O)H group.
[0078] The term "formylalkyl", as used herein, means a formyl group, as defined herein, appended to the parent molecular moiety through an alkyl group, as defined herein.
Representative examples of formylalkyl include, but are not limited to, formylmethyl and 2-formylethyl.
[0079] The term "halo" or "halogen", as used herein, means -Cl, -Br, -I or -F.
[0080] The term "haloalkoxy", as used herein, means at least one halogen, as defined herein, appended to the parent molecular moiety through an alkoxy group, as defined herein.
Representative examples of haloalkoxy include, but are not limited to, chloromethoxy, 2-fluoroethoxy, trifluoromethoxy, and pentafluoroethoxy.
[0081] The term "haloalkyl", as used herein, means at least one halogen, as defined herein, appended to the parent molecular moiety through an alkyl group, as defined herein.
Representative examples of haloalkyl include, but are not limited to, chloromethyl, 2-fluoroethyl, trifluoromethyl, pentafluoroethyl, and 2-chloro-3-fluoropentyl.
[0082] The term "haloalkylcarbonyl", as used herein, means a haloalkyl group, as defined herein, appended to the parent molecular moiety through a carbonyl group, as defined herein.
Representative examples of haloalkylcarbonyl include, but are not limited to, triflhoromethylcarbonyl and trifluoromethylcarbonyl.
[0083] The term "heteroaryl", as used herein, means a monocyclic heteroaryl or a bicyclic heteroaryl. The monocyclic heteroaryl is a 5 or 6 membered ring that contains at least one heteroatom selected from the group consisting of nitrogen, oxygen and sulfur.
The 5 membered ring contains two double bonds and the 6 membered ring contains three double bonds. The 5 or 6 membered heteroaryl is connected to the parent molecular moiety through any carbon atom or any substitutable nitrogen atom contained within the heteroaryl, provided that proper valance is maintained. Representative examples of monocyclic heteroaryl include, but are not limited to, furyl, imidazolyl, isoxazolyl, isothiazolyl, oxadiazolyl, oxazolyl, pyridinyl, pyridazinyl, pyrimidinyl, pyrazinyl, pyrazolyl, pyrrolyl, tetrazolyl, thiadiazolyl, thiazolyl, thienyl, triazolyl, and triazinyl. The bicyclic heteroaryl consists of a monocyclic heteroaryl fused to a phenyl, or a monocyclic heteroaryl fused to a cycloalkyl, or a monocyclic heteroaryl fused to a cycloalkenyl, or a monocyclic heteroaryl fused to a monocyclic heteroaryl, or a monocyclic heteroaryl fused to a monocyclic heterocycle. The bicyclic heteroaryl is connected to the parent molecular moiety through any carbon atom or any substitutable nitrogen atom contained within the bicyclic heteroaryl, provided that proper valance is maintained. Representative examples of bicyclic heteroaryl include, but are not limited to, azaindolyl, benzimidazolyl, benzo[d][1,3]dioxolyl, benzofuranyl, benzoxadiazolyl, benzo[d]imidazolyl, benzo[d]imidazle-2(3H)-thione, benzoisoxazole, benzoisothiazole, benzooxazole, benzooxazolone, benzo[d][1,2,3]thiadiazolyl, 1,3-benzothiazolyl, benzothiophenyl, benzo[d][1,2,3]triazolyl, cinnolinyl, 2,2-difluorobenzo[d][1,3]dioxolyl, furopyridine, imidazopyridinyl, indolyl, indazolyl, isobenzofuran, isoindolyl, isoquinolinyl, naphthyridinyl, oxazolopyridine, pyrazolopyrimidinyl, pyrrolopyridinyl, quinolinyl, quinoxalinyl, thienopyridinyl, and [1,2,4]triazolopyridinyl.
[0084] The heteroaryl groups of the invention are optionally substituted with 1, 2, 3 or 4 substituents independently selected from the group consisting of alkenyl, alkoxy, alkoxyalkoxy, alkoxyalkyl, alkoxycarbonyl, alkoxycarbonylalkyl, alkoxysulfonyl, alkyl, alkylcarbonyl, alkylcarbonylalkyl, alkylcarbonyloxy, alkylthio, alkylthioalkyl, alkynyl, carboxy, carboxyalkyl, cyano, cyanoalkyl, formyl, haloalkoxy, haloalkyl, halogen, hydroxy, hydroxyalkyl, mercapto, nitro, -NZ'Z2, (NZ3Z4)carbonyl and oxo. Heteroaryl groups of the invention that are substituted with a hydroxyl group may be present as tautomers. The heteroaryl groups of the invention encompass all tautomers including non-aromatic tautomers.
[0085] The term "heterocycle" or "heterocyclic", as used herein, means a monocyclic heterocycle, a bicyclic heterocycle or a tricyclic heterocycle. The monocyclic heterocycle is a 3, 4, 5, 6 or 7 membered ring containing at least one heteroatom independently selected from the group consisting of 0, N, and S. The 3 or 4 membered ring contains 1 heteroatom selected from the group consisting of 0, N and S. The 5 membered ring contains zero or one double bond and one, two or three heteroatoms selected from the group consisting of 0, N
and S. The 6 or 7 membered ring contains zero, one or two double bonds and one, two or three heteroatoms selected from the group consisting of 0, N and S. The monocyclic heterocycle is connected to the parent molecular moiety through any carbon atom or any nitrogen atom contained within the monocyclic heterocycle. Representative examples of monocyclic heterocycle include, but are not limited to, azetidinyl, azepanyl, aziridinyl, diazepanyl, 1,3-dioxanyl, 1,3-dioxolanyl, 1,3-dithiolanyl, 1,3-dithianyl, imidazolinyl, imidazolidinyl, isothiazolinyl, isothiazolidinyl, isoxazolinyl, isoxazolidinyl, morpholinyl, oxadiazolinyl, oxadiazolidinyl, oxazolinyl, oxazolidinyl, piperazinyl, piperidinyl, pyranyl, pyrazolinyl, pyrazolidinyl, pyrrolinyl, pyrrolidinyl, tetrahydrofuranyl, tetrahydrothienyl, thiadiazolinyl, thiadiazolidinyl, thiazolinyl, thiazolidinyl, thiomorpholinyl, 1,1-dioxidothiomorpholinyl (thiomorpholine sulfone), thiopyranyl, and trithianyl.
The bicyclic heterocycle is a 5 or 6 membered monocyclic heterocycle fused to a phenyl group, or a 5 or 6 membered monocyclic heterocycle fused to a cycloalkyl, or a 5 or 6 membered monocyclic heterocycle fused to a cycloalkenyl, or a 5 or 6 membered monocyclic heterocycle fused to a monocyclic heterocycle. The bicyclic heterocycle is connected to the parent molecular moiety through any carbon atom or any nitrogen atom contained within the bicyclic heterocycle. Representative examples of bicyclic heterocycle include, but are not limited to, 1,3-benzodioxolyl, 1,3-benzodithiolyl, 2,3-dihydro-1,4-benzodioxinyl, benzodioxolyl, 2,3-dihydro-l-benzofuranyl, 2,3-dihydro-l-benzothienyl, chromenyl and 1,2,3,4-tetrahydroquinolinyl. The tricyclic heterocycle is a bicyclic heterocycle fused to a phenyl, or a bicyclic heterocycle fused to a cycloalkyl, or a bicyclic heterocycle fused to a cycloalkenyl, or a bicyclic heterocycle fused to a monocyclic heterocycle. The tricyclic heterocycle is connected to the parent molecular moiety through any carbon atom or any nitrogen atom contained within the tricyclic heterocycle. Representative examples of tricyclic heterocycle include, but are not limited to, 2,3,4,4a,9,9a-hexahydro-lH-carbazolyl, 5a,6,7,8,9,9a-hexahydrodibenzo[b,d]furanyl, and 5a,6,7,8,9,9a-hexahydrodibenzo[b,d]thienyl.
[0086] The heterocycles of this invention are optionally substituted with 1, 2, 3 or 4 substituents independently selected from the group consisting of alkenyl, alkoxy, alkoxyalkoxy, alkoxyalkyl, alkoxycarbonyl, alkoxycarbonylalkyl, alkoxysulfonyl, alkyl, alkylcarbonyl, alkylcarbonylalkyl, alkylcarbonyloxy, alkylthio, alkylthioalkyl, alkynyl, carboxy, carboxyalkyl, cyano, cyanoalkyl, formyl, haloalkoxy, haloalkyl, halogen, hydroxy, hydroxyalkyl, nitro, mercapto, oxo, -NZ'Z2 and (NZ3Z4)carbonyl.
[0087] The term "hydroxy", as used herein, means an -OH group.
[0088] The term "hydroxyalkyl", as used herein, means at least one hydroxy group, as defined herein, is appended to the parent molecular moiety through an alkyl group, as defined herein. Representative examples of hydroxyalkyl include, but are not limited to, hydroxymethyl, 2-hydroxyethyl, 3-hydroxypropyl, 2,3-dihydroxypentyl, and 2-ethyl-4-hydroxyheptyl.
[0089] The term "hydroxy-protecting group" or "O-protecting group" means a substituent which protects hydroxyl groups against undesirable reactions during synthetic procedures.
Examples of hydroxy-protecting groups include, but are not limited to, substituted methyl ethers, for example, methoxymethyl, benzyloxymethyl, 2-methoxyethoxymethyl, 2-(trimethylsilyl)-ethoxymethyl, benzyl, and triphenylmethyl; tetrahydropyranyl ethers;
substituted ethyl ethers, for example, 2,2,2-trichloroethyl and t-butyl; silyl ethers, for example, trimethylsilyl, t-butyldimethylsilyl and t-butyldiphenylsilyl; cyclic acetals and ketals, for example, methylene acetal, acetonide and benzylidene acetal;
cyclic ortho esters, for example, methoxymethylene; cyclic carbonates; and cyclic boronates.
Commonly used hydroxy-protecting groups are disclosed in T.W. Greene and P.G.M. Wuts, Protective Groups in Organic Synthesis, 3rd edition, John Wiley & Sons, New York (1999).
[0090] The term "lower alkenyl", as used herein, is a subset of alkenyl, as defined herein, and means an alkenyl group containing from 2 to 4 carbon atoms. Examples of lower alkenyl are ethenyl, propenyl, and butenyl.
[0091] The term " lower alkoxy", as used herein, is a subset of alkoxy, as defined herein, and means a lower alkyl group, as defined herein, appended to the parent molecular moiety through an oxygen atom, as defined herein. Representative examples of lower alkoxy include, but are not limited to, methoxy, ethoxy, propoxy, 2-propoxy, butoxy, and tert-butoxy.
[0092] The term "lower alkyl", as used herein, is a subset of alkyl, as defined herein, and means a straight or branched chain hydrocarbon group containing from 1 to 4 carbon atoms.
Examples of lower alkyl are methyl, ethyl, n-propyl, iso-propyl, n-butyl, iso-butyl, sec-butyl, and tert-butyl.
[0093] The term "lower haloalkoxy", as used herein, is a subset of haloalkoxy, as defined herein, and means a straight or branched chain haloalkoxy group containing from 1 to 4 carbon atoms. Representative examples of lower haloalkoxy include, but are not limited to, trifluoromethoxy, trichloromethoxy, dichloromethoxy, fluoromethoxy, and pentafluoroethoxy.
[0094] The term "lower haloalkyl", as used herein, is a subset of haloalkyl, as defined herein, and means a straight or branched chain haloalkyl group containing from 1 to 4 carbon atoms. Representative examples of lower haloalkyl include, but are not limited to, trifluoromethyl, trichloromethyl, dichloromethyl, fluoromethyl, and pentafluoroethyl.
[0095] The term "mammal" includes humans and animals, such as cats, dogs, swine, cattle, horses, and the like.
[0096] The term "methylenedioxy", as used herein, means a -OCH2O- group wherein the oxygen atoms of the methylenedioxy are attached to the parent molecular moiety through two adjacent carbon atoms.
[0097] The term "nitrogen protecting group", as used herein, means those groups intended to protect an amino group against undesirable reactions during synthetic procedures.
Preferred nitrogen protecting groups are acetyl, benzoyl, benzyl, benzyloxycarbonyl (Cbz), formyl, phenylsulfonyl, tert-butoxycarbonyl (Boc), tert-butylacetyl, trifluoroacetyl, and triphenylmethyl (trityl).
[0098] The term "mercapto", as used herein, means a -SH group.
[0099] The term "nitro", as used herein, means a -NO2 group.
[00100] The term "NZ'Z2", as used herein, means two groups, Z' and Z2, which are appended to the parent molecular moiety through a nitrogen atom. Z' and Z2 are each independently selected from the group consisting of hydrogen, alkyl, alkylcarbonyl, alkoxycarbonyl, aryl, arylalkyl, and formyl. In certain instances within the invention, Z' and z2 taken together with the nitrogen atom to which they are attached form a heterocyclic ring.
Representative examples of NZ1Z2 include, but are not limited to, amino, methylamino, acetylamino, acetylmethylamino, phenylamino, benzylamino, azetidinyl, pyrrolidinyl and piperidinyl.
[00101] The term "NZ3Z4", as used herein, means two groups, Z3 and Z4, which are appended to the parent molecular moiety through a nitrogen atom. Z3 and Z4 are each independently selected from the group consisting of hydrogen, alkyl, aryl and arylalkyl.
Representative examples of NZ3Z4 include, but are not limited to, amino, methylamino, phenylamino and benzylamino.
[00102] The term "oxo", as used herein, means a =0 moiety.
[00103] The term "pharmaceutically acceptable amide," as used herein, refers to those amides, which retain, upon hydrolysis of the amide bond, the biological effectiveness and properties of the carboxylic acid and are not biologically or otherwise undesirable.
Pharmaceutically acceptable amides of the invention can be derived from ammonia, primary C1_6 alkyl amines and secondary C1_6 dialkyl amines. In the case of secondary amines, the amine can also be in the form of a 5- or 6-membered heterocycle containing one nitrogen atom. Amides derived from ammonia, C1.3 alkyl primary amides and C1_2 dialkyl secondary amides are preferred. Amides of the compounds of formulas (I) and (II) can be prepared according to conventional methods. Pharmaceutically acceptable amides can be prepared from compounds containing primary or secondary amine groups by reaction of the compound that contains the amino group with an alkyl anhydride, aryl anhydride, acyl halide, or aroyl halide. In the case of compounds containing carboxylic acid groups, the pharmaceutically acceptable esters are prepared from compounds containing the carboxylic acid groups by reaction of the compound with base such as triethylamine, a dehydrating agent such as dicyclohexyl carbodiimide or carbonyl diimidazole, and an alkyl amine, dialkylamine, for example with methylamine, diethylamine, piperidine. They also can be prepared by reaction of the compound with an acid such as sulfuric acid and an alkylcarboxylic acid such as acetic acid, or with acid and an arylcarboxylic acid such as benzoic acid under dehydrating conditions as with molecular sieves added. The composition can contain a compound of the invention in the form of a pharmaceutically acceptable prodrug.
[00104] The term "pharmaceutically acceptable prodrug" or "prodrug" as used herein, represents those prodrugs of the compounds of the invention which are, within the scope of sound medical judgment, suitable for use in contact with the tissues of humans and lower animals without undue toxicity, irritation, allergic response, and the like, commensurate with a reasonable benefit/risk ratio, and effective for their intended use.
Prodrugs of the invention can be rapidly transformed in vivo to a parent compound of the invention, for example, by hydrolysis in blood. A thorough discussion is provided in Higuchi T., et al., Pro-drugs as Novel Delivery Systems, V. 14 of the A.C.S. Symposium Series, and in Edward B.
Roche, ed., Bioreversible Carriers in Drug Design, American Pharmaceutical Association and Pergamon Press (1987).
[00105] The term "pharmaceutically acceptable carrier" as used herein, means a non-toxic, solid, semi-solid or liquid filler, diluent, encapsulating material, or formulation auxiliary of any type. Examples of therapeutically suitable excipients include sugars;
cellulose and derivatives thereof; oils; glycols; solutions; buffering, coloring, releasing, coating, sweetening, flavoring, and perfuming agents; and the like. These therapeutic compositions can be administered parenterally, intracisternally, orally, rectally, intraveneously, or intraperitoneally.
[00106] The term "sulfinyl", as used herein, means a -S(O)- group."
[00107] The term "sulfonamide", as used herein means an amino, alkylamino, or dialkylamino group, as defined herein, appended to the parent molecular moiety through a sulfonyl group, as defined herein. Representative examples include, but are not limited to, aminosulfonyl, methylaminosulfonyl, and diethylaminosulfonyl.
[00108] The term "sulfonyl", as used herein, means a -SO2- group.
[00109] The term "tautomer", as used herein, means a proton shift from one atom of a compound to another atom of the same compound wherein two or more structurally distinct compounds are in equilibrium with each other.
[00110] The term "radiolabel" refers to a compound in which at least one of the atoms is a radioactive atom or radioactive isotope, wherein the radioactive atom or isotope spontaneously emits gamma rays or energetic particles, for example alpha particles or beta particles, or positrons. Examples of such radioactive atoms include, but are not limited to, 3H

(tritium), 14C, 11C, 150, 18F, 35S, 123I, and 125I. Compounds suitable for the composition, method, and article of manufacture for the invention are any chemical compounds for which a4(32 nicotinic receptor activity can be identified.
[00111] The term "treatment" or "treating" includes any process, action, application, therapy, or the like, wherein a subject, including human, is provided medical aid with the object of improving the subject's condition, directly or indirectly, or slowing the progression of a condition or disorder in the subject.
[00112] Although typically it maybe recognized that an asterisk is used to indicate that the exact subunit composition of a receptor is uncertain, for example a4(32*
indicates a receptor that contains thea4 and (32subunit proteins in combination with other subunits.
[00113] It has been found that the efficacy of nicotinic receptor ligands surprisingly can be improved by combining a nicotinic acetylcholine receptor ligand, particularly an a4(32 receptor ligand (agonist, partial agonist), with a nicotinic acetylcholine receptor a4(32 subtype selective positive allosteric modulator (PAM). Such combinations are highly efficient for improving the efficacy of a4(32 ligand for treatment of pain and other disease indications such as cognitive deficits when compared to administration of an a4(32 receptor ligand alone.

Nicotinic Acetylcholine Subtype a4(32 Receptor Ligands _ 20 [00114] Nicotinic acetylcholine subtype a4(32 receptor ligands modulate the function by altering the activity of the receptor. Suitable compounds also can be partial agonists that partially block or partially activate the a4(32 receptor or agonists that activate the receptor.
Nicotinic acetylcholine receptor a4(32 receptor ligands suitable for the invention can include full agonists or partial agonists. Compounds modulating activity of nicotinic acetylcholine receptor a4(32 subtype are suitable for the invention regardless of the manner in which they interact with the receptor.

[00115] One manner for characterizing a4(32 receptor ligands is by a binding assay. [3H]-Cytisine binding values ("K; Cyt") of compounds of the invention ranged from about 0.001 nanomolar to greater than 100 micromolar. Preferred compounds for the composition demonstrate binding values of from about 0.001 nanomolar to 10 micromolar. The [3H]-cytisine binding assays have been well reported; however, further details for carrying out the assays can be obtained in International Publication No. WO 99/32480; U.S.
Patent Nos.

5,948,793 and 5,914,328; WO 2004/018607; U.S. Patent No. 6,809,105; WO
00/71534; and U.S. Patent No. 6,833,370.

[00116] Accordingly, a4(32 receptor ligands suitable for the invention can be compounds of various chemical classes. Particularly, some examples of a4(32 receptor ligands suitable for the invention include, but are not limited to heterocyclic ether derivatives (see, for example, International Publication No. WO 99/32480, published July 1, 1999; U.S. Patent No.
5,948,793, issued September 7, 1999, and U.S. Patent No 5,914,328, issued June 22, 1999);
N-substituted diazabicyclic derivatives (see for exampleInternational Publication No. WO
2004/0186107, published September 23, 2004, and U.S. Patent No. 6,809,105, issued October 26, 2004); heterocyclic substituted amino azacycles (see for example, International Publication No. WO 00/71534, published November 30, 2000, and U.S. Patent No.
6,833,370, issued December 21, 2004); all of which are hereby incorporated by reference in their entirety. Further description and methods for preparing the compounds have been reported in the patents, patent publications, and international patent publications cited.

[00117] Additional examples of a4(32 receptor ligands suitable for the invention include, but are not limited to aryl-fused azapolycyclic compounds (see for example, International Publication No. WO 2001062736, published August 30, 2001); aryl-substituted olefinic amine compounds (see for example, International Publication Nos. WO 9965876, published December 23, 1999, and WO 00/75110, published December 14, 2000);
pyridopyranoazepine derivatives (see for example, US Patent No. 6,538,003, published March 25, 2003);
benzylidene- and cinnamylidene- anabaseines (see for examples, International Publication No. WO 99/10338, published March 4, 1999); and 3-pyridoxylalkyl heterocyclic ether compounds (see for example, International Publication No. WO 96/040682, published December 19, 1996); all of which are hereby incorporated by reference in their entirety.
Further description and methods for preparing the compounds have been reported in the patents and international patent publications cited.

[00118] Other compounds reported as demonstrating a4(32 ligands include, but are not limited to, TC-1734 (ispronicline), GTS-21, 4-hydroxy-GTS-21, TC-5619, TC-2696, dianicline and varenicline, which are all described in the publicly available literature.

[00119] Specific examples of compounds contemplated for the a4(32 receptor ligands include, but are not limited to, [00120] 5-[(2R)-azetidin-2-ylmethoxy]-2-chloropyridine;
[00121] (3R)-l-pyridin-3-ylpyrrolidin-3-amine;

[00122] 2-methyl-3-(2-(S)-pyrrolidinylmethoxy)pyridine;
[00123] 3-(5,6-dichloro-pyridin-3-yl)-1S,5S-3,6-diazabicyclo[3.2.0]heptane;
[00124] (R,R)-1-(pyridin-3-yl)octahydro-pyrrolo[3,4-b]pyrrole;
[00125] 6,10-methano-6H-pyrazino[2,3-h][3]benzazepine;
[00126] 7,8,9,10-tetrahydro-(2S,4E)-N-methyl-5-(5-isopropoxy-3-pyridyl)-4-penten-2-amine;
[00127] (2S,4E)-N-methyl-5-(5-methoxy-3-pyridyl)-4-penten-2-amine;
[00128] (2S,4E)-N-methyl-5-(5-ethoxy-3-pyridyl)-4-penten-2-amine;
[00129] (2S,4E)-N-methyl-3-pyrimidine-4-penten-2-amine;
[00130] (5aS,8S,lOaR)-5a,6,9,10-tetrahydro-7H,11H-8,10a-methanopyrido[2',3':5,6]pyrano[2,3-d]azepine;
[00131] 3-[1-(2,4-dimethoxy-phenyl)-meth-(E)-ylidene]-3,4,5,6-tetrahydro-[2,3']bipyridinyl; and [00132] 3-[1-(2-methoxy-4-hydroxyphenyl)-meth-(E)-ylidene]-3,4,5,6-tetrahydro-[2,3']bipyridinyl;
[00133] or pharmaceutically acceptable salts thereof.

Nicotinic Acetylcholine Subtype a4132 Receptor Positive Allosteric Modulators [00134] Positive allosteric modulators (PAMs) are compounds that potentiate receptor responses to acetylcholine without themselves triggering receptor activation or desensitization, or either, of the receptor.

[00135] One manner for characterizing a4(32 positive allosteric modulator activity is by characterization in human HEK cells expressing the human nicotinic acetylcholine receptor subtype a4(32, particularly by use of Fluorescent Image Plate Reader technology. Such assay has been reported and further details for carrying out the assays can be obtained in International Publication Nos. WO 2006/114400, published November 2, 2006.
Another method to identify and characterize allosteric modulator activity is by expressing the a4(32 subunits in Xenopus oocytes or cell lines, and by measuring effects on ligand-evoked current responses as previously described (Curtis L, et al., Molecular Pharmacology, 61: 127-135, 2002).
[00136] Steroid hormones represent a family of molecules with varying modulatory effects onnAChRs as well as other members of the LGIC superfamily. For example, positive allosteric modulation of human a4(32 nAChRs expressed either in Xenopus oocytes or in human embryonic kidney cells was reported with 17 (3-estradiol (Curtis L, et al., Molecular Pharmacology, 61: 127-135, 2002). Examples of compounds reported as selective a4(32 positive allosteric modulators are oxadiazole derivatives, for example as described in WO
2006/114400.

[00137] Another suitable a4(32 positive allosteric modulator is 3,5-diphenylisoxazole, which is commercially available from Sigma Aldrich, St. Louis, Missouri, USA.

[00138] Other suitable examples of a4132 positive allosteric modulators include, but are not limited to, oxadiazole derivatives. Suitable oxadiazole derivatives can include 1,2,4-oxadiazole derivatives and 1,3,4-oxadiazole derivatives. Examples of 1,3,4-oxadiazole derivatives are described in co-pending U.S. Patent Application No.
61/000,295, filed on April 12, 2007, wherein the methods of preparation disclosed are incorporated by reference herein. Such compounds have the formula (I):

N-N
1 -J,/
Ar 0 X.Y
(I) [00139] or are pharmaceutically acceptable salts and prodrugs thereof, wherein X is a bond, 0, NR', S, or CI-C3 alkylene;
Y represents a monocyclic aryl, cycloalkyl, heterocycle, or heteroaryl group;
Ari represents a monocyclic aryl or a heteroaryl group; and R1 is hydrogen, alkyl, haloalkyl or arylalkyl.
[00140] X is selected from a bond, 0, NR', S, or CI-C3 alkylene, wherein R1 is selected from hydrogen, alkyl, haloalkyl, and arylalkyl. Preferably, X is a bond.
Preferably, R1 is hydrogen or alkyl.
[00141] Y represents a monocyclic aryl, cycloalkyl, heterocycle, or heteroaryl group, which can be substituted or unsubstituted with substituents. Examples of suitable heterocycle groups can include, but are not limited to, pyrrolidine, piperidine, and the like. Examples of suitable heteroaryl groups can include, but are not limited to, thienyl, furanyl, pyridinyl, pyrazinyl, and the like. A preferred monocyclic aryl group is substituted or unsubstituted phenyl. Suitable substituents for the monocyclic aryl, heterocycle, or heteroaryl group are, for example, alkyl, cycloalkyl, cycloalkylalkyl, halo, haloalkyl, hydroxyl, alkoxy, haloalkoxy, nitro, and cyan.

[00142] Ari represents a monocyclic aryl, such as substituted or unsubstituted phenyl, or heteroaryl group. Examples of suitable heteroaryl groups include, but are not limited, thienyl, furanyl, pyrrolyl, pyrazolyl, thiazolyl, 1,3,4-thiadiazolyl, and pyridinyl, each of which can be unsubstituted or substituted with one, two, or three substituents selected from alkyl, cycloalkyl, cycloalkylalkyl, halo, haloalkyl, hydroxyl, alkoxy, haloalkoxy, nitro, cyano, and amino.
[00143] In one embodiment, suitable 2,5-disubstituted-1,3,4-oxadiazole derivatives can have the formula (I) wherein X is a bond; Y is aryl, cycloalkyl, heterocycle, or heteroaryl;
and Ari is monocyclic aryl or heteroaryl.
[00144] In another embodiment, suitable 2,5-disubstituted-1,3,4-oxadiazole derivatives can have the formula (I) wherein X is a bond; Y is monocyclic cycloalkyl, phenyl, thienyl, furyl, pyridinyl, pyrazinyl, pyrrolidinyl, or piperidinyl optionally substituted with one or more of the substituents selected from the group consisting of alkyl, halogen, haloalkyl, hydroxy, alkoxy, haloalkoxy, nitro and cyan; and Ari is phenyl, thienyl, furyl, pyrrolyl, pyrazolyl, thiazolyl, 1,3,4-thiadiazolyl, pyrimidinyl, pyrazinyl, or pyridinyl optionally substituted with one or more of the substituents selectected from the group consisting of alkyl, alkylcarbonyl, alkylsulfonyl, alkythio, alrylalkyl, aryloxy, arylalkyloxy, halogen, haloalkyl, hydroxy, alkoxy, haloalkoxy, nitro, cyan, and NZ'Z2, wherein Z' and Z2 are hydrogen, alkyl, alkylcarbonyl, alkoxycarbonyl, aryl, arylalkyl, or formyl.
[00145] In another embodiment, the suitable 2,5-disubstituted-1,3,4-oxadiazole derivatives can have the formula (I) wherein X is a bond; Y is pyridyl; and Ari is phenyl, pyrimidinyl, pyrazinyl, or pyridinyl optionally substituted with one or more of the substituents selected from the group consisting of alkyl, halogen, haloalkyl, hydroxy, alkoxy, haloalkoxy, nitro, cyan, and NZ'Z2, wherein Z' and Z2 are hydrogen, alkyl, alkylcarbonyl, alkoxycarbonyl, aryl, arylalkyl, or formyl.

[00146] Other suitable examples of compounds reported as a4(32 positive allosteric modulators are oxadiazole derivatives, for example as described in WO
2006/114400, published November 2, 2006. Further examples of oxadiazole compounds that are suitable as a4(32 positive allosteric modulators are also provided in WO 02/100826, published December 19, 2002.

[00147] Yet other suitable examples of a4132 positive allosteric modulators include, but are not limited to, compounds of the formula (II):

Ar2N -Ara O-N
(II) or are pharmaceutically acceptable salts thereof, wherein [00148] Ar 2 is aryl or heteroaryl, wherein the aryl or heteroaryl is substituted or unsubstituted, and, when substituted, the aryl or heteroaryl is substituted with 0, 1, 2, 3, or 4 substituents selected from halo, C1-C6 haloalkyl, C6-C10 aryl, C4-C7 cycloalkyl, C2-C6 alkenyl, C2-C6 alkynyl, C5-C10 heteroaryl, C4-Cio heterocycle, C1-C6 alkyl, -(C1-C6 alkyl)NHC(O)O-(Ci-C6 alkyl), C1-C6 hydroxyalkyl, C1-C6 alkylcarbonyl, amino, hydroxyl, haloalkyl-C(O)-, haloalkyl-S02-, alkyl-S02-, -SO2NH2, -SO2NH(C1-C6 alkyl), -SO2N(C1-C6 alkyl)2, cyan, nitro, C1-C6 acylamino, C1-C6 alkoxy, -C(O)NH2, -C(O)O-(C1-C6 alkyl), and carboxy; and [00149] Ar 3 is aryl or heteroaryl, wherein the aryl or heteroaryl is optionally substituted, and, when substituted, the aryl or heteroaryl is substituted with a substituent selected from halo, C1-C6 haloalkyl, C6-C10 aryl, C4-C7 cycloalkyl, C2-C6 alkenyl, C2-C6 alkynyl, C5-C10 heteroaryl, C1-C6 alkyl, C1-C6 hydroxyalkyl, amino, hydroxyl, haloalkyl-S02-, cyan, nitro, C1-C6 acylamino, C1-C6 alkoxy, -N(C1-C6 alkyl)2, and carboxy.
[00150] In one embodiment, suitable 3,5-disubstituted-1,2,4-oxadiazole derivatives can have the formula (I) wherein Ar 2 is substituted monocyclic aryl or monocyclic heteroaryl, which can be substituted or unsubstituted, and Ar 3 is substituted monocyclic aryl or heteroaryl, which can be substituted or unsubstituted. When the aryl or heteroaryl group for Ar 2 is substituted the substituent is selected from halo, C1-C6 haloalkyl, C6-C10 aryl, C4-C7 cycloalkyl, C2-C6 alkenyl, C2-C6 alkynyl, C5-C10 heteroaryl, C4-Ci0 heterocycle, C1-C6 alkyl, -(C1-C6 alkyl)NHC(O)O-(CI-C6 alkyl), C1-C6 hydroxyalkyl, C1-C6 alkylcarbonyl, amino, hydroxyl, haloalkyl-C(O)-, haloalkyl-S02-, alkyl-S02-, -SO2NH2, -SO2NH(C1-C6 alkyl), -SO2N(C1-C6 alkyl)2, cyan, nitro, C1-C6 acylamino, C1-C6 alkoxy, -C(O)NH2, -C(O)O-(C1-C6 alkyl), and carboxy. When the aryl or heteroaryl group for Ar 3 is substituted the substituent is selected from halo, C1-C6 haloalkyl, C6-C10 aryl, C4-C7 cycloalkyl, C2-C6 alkenyl, C2-C6 alkynyl, C5-C10 heteroaryl, C1-C6 alkyl, C1-C6 hydroxyalkyl, amino, hydroxyl, haloalkyl-S02-, cyan, nitro, C1-C6 acylamino, C1-C6 alkoxy, -N(C1-C6 alkyl)2, and carboxy.
Preferred for monocyclic heteroaryl are pyridine-3-yl, pyridine-4-yl, and pyridine-2(1H)-one.
[00151] In another embodiment, suitable 3,5-disubstituted-1,2,4-oxadiazole derivatives can have the formula (II) wherein wherein Ar2 is pyridinyl, which can be substituted or unsubstituted, or substituted phenyl; and Ar 3 is pyridinyl, which can be substituted or unsubstituted, or substituted phenyl. The pyridinyl group, when substituted, is substituted with fluoro. The phenyl group is substituted with cyano or halo. It is preferred that the pyridinyl group for Ar 2 or Ar 3 is pyridin-3-yl. The preferred phenyl group is substitute with fluoro, sulfonamide or cyano, and preferably cyano.

[00152] Specific examples of a4(32 positive allosteric modulators are, for example, 3,5-disubstituted-1,2,4-oxadiazole derivatives, such as:
[00153] 3-(3-(pyridin-3-yl)-1,2,4-oxadiazol-5-yl)benzonitrile;
[00154] 3,5-di(pyridin-3-yl)-1,2,4-oxadiazole;
[00155] 3-(5-(pyridin-3-yl)-1,2,4-oxadiazol-3-yl)benzonitrile;
[00156] 3-(5-(6-fluoropyridin-3-yl)-1,2,4-oxadiazol-3-yl)benzonitrile;
[00157] 5-(5-bromopyridin-3-yl)-3-(pyridin-3-yl)-1,2,4-oxadiazole;
[00158] 3-(pyridin-3-yl)-5-(3-(trifluoromethylsulfonyl)phenyl)-1,2,4-oxadiazole;
[00159] 3-(3-(6-methylpyridin-3-yl)-1,2,4-oxadiazol-5-yl)benzonitrile;
[00160] 5-(5-(pyrrol-1-yl)pyridin-3-yl)-3-(pyridin-3-yl)-1,2,4-oxadiazole;
[00161] 5-(3-(pyridin-3-yl)-1,2,4-oxadiazol-5-yl)pyridin-3-ol;
[00162] 5-(3,4-difluorophenyl)-3-(pyridin-3-yl)-1,2,4-oxadiazole;
[00163] 5-(2,3-difluorophenyl)-3-(pyridin-3-yl)-1,2,4-oxadiazole;
[00164] 5-(pyrazin-2-yl)-3-(pyridin-3-yl)-1,2,4-oxadiazole;
[00165] 5-(3,5-difluorophenyl)-3-(pyridin-3-yl)-1,2,4-oxadiazole;
[00166] 5-(2,3,5-trifluorophenyl)-3-(pyridin-3-yl)-1,2,4-oxadiazole;
[00167] 5-(2,4,5-trifluorophenyl)-3-(pyridin-3-yl)-1,2,4-oxadiazole;
[00168] 5-(2,5-difluorophenyl)-3-(pyridin-3-yl)-1,2,4-oxadiazole;
[00169] 5-(4-chloro-2,5-difluorophenyl)-3-(pyridin-3-yl)-1,2,4-oxadiazole;
[00170] 5-(5-methylpyrazin-2-yl)-3-(pyridin-3-yl)-1,2,4-oxadiazole;
[00171] 4-(3-(pyridin-3-yl)-1,2,4-oxadiazol-5-yl)benzonitrile;
[00172] 2,3,6-trifluoro-5-(3-(pyridin-3-yl)-1,2,4-oxadiazol-5-yl)phenol;
[00173] 2-fluoro-5-(3-(pyridin-3-yl)-1,2,4-oxadiazol-5-yl)phenol;
[00174] 2-fluoro-4-(3-(pyridin-3-yl)-1,2,4-oxadiazol-5-yl)phenol;
[00175] 5-(3-chloro-4-fluorophenyl)-3-(pyridin-3-yl)-1,2,4-oxadiazole;
[00176] 5-(3,4-dichlorophenyl)-3-(pyridin-3-yl)-1,2,4-oxadiazole;
[00177] 2-nitro-5-(3-(pyridin-3-yl)-1,2,4-oxadiazol-5-yl)phenol;
[00178] 5-(2,3,6-trifluorophenyl)-3-(pyridin-3-yl)-1,2,4-oxadiazole;
[00179] 2,2,2-trifluoro-l-(4-(3-(pyridin-3-yl)-1,2,4-oxadiazol-5-yl)phenyl)ethanone;

[00180] 5-(3-fluorophenyl)-3-(pyridin-3-yl)-1,2,4-oxadiazole;
[00181] 5-(4-fluorophenyl)-3-(pyridin-3-yl)-1,2,4-oxadiazole;
[00182] 5-(2-fluorophenyl)-3-(pyridin-3-yl)-1,2,4-oxadiazole;
[00183] 3-fluoro-5-(3-(pyridin-3-yl)-1,2,4-oxadiazol-5-yl)benzonitrile;
[00184] 3 -(2,3 -difluorophenyl)-5 -(pyridin-3 -yl)- 1,2,4-oxadiazole;
[00185] 3 -(3,4-difluorophenyl)-5 -(pyridin-3 -yl)- 1,2,4-oxadiazole;
[00186] 5 -(2,6-difluorophenyl)-3 -(pyridin-3 -yl)- 1,2,4-oxadiazole;
[00187] 3-(3-(pyridin-3-yl)-1,2,4-oxadiazol-5-yl)benzenesulfonamide;
[00188] 5 -(2,4-difluorophenyl)-3 -(pyridin-3 -yl)- 1,2,4-oxadiazole;
[00189] 5-(2,3,4-trifluorophenyl)-3-(pyridin-3-yl)-1,2,4-oxadiazole;
[00190] 5-(3,4,5-trifluorophenyl)-3-(pyridin-3-yl)-1,2,4-oxadiazole;
[00191] 5-(4-chloro-3-fluorophenyl)-3-(pyridin-3-yl)-1,2,4-oxadiazole;
[00192] 5 -(3 -nitrophenyl)-3 -(pyridin-3 -yl)- 1,2,4-oxadiazole;
[00193] 5-(3-(methylsulfonyl)phenyl)-3-(pyridin-3-yl)-1,2,4-oxadiazole;
[00194] 3 -(2-chloropyridin-4-yl)-5 -(pyridin-3 -yl)- 1,2,4-oxadiazole;
[00195] 3-(3-(pyridin-3-yl)-1,2,4-oxadiazol-5-yl)benzamide;
[00196] 4-(5-(pyridin-3-yl)-1,2,4-oxadiazol-3-yl)pyridin-2(1H)-one;
[00197] tent-butyl 3-(3-(pyridin-3-yl)-1,2,4-oxadiazol-5-yl)benzoate;
[00198] 2-amino-5-(3-(pyridin-3-yl)-1,2,4-oxadiazol-5-yl)phenol;
[00199] N,N-dimethyl-4-(5-(pyridin-3-yl)-1,2,4-oxadiazol-3-yl)pyridin-2-amine;
[00200] 3-(3-(pyridin-3-yl)-1,2,4-oxadiazol-5-yl)benzoic acid;
[00201] 5-(3-(1H-tetrazol-5-yl)phenyl)-3-(pyridin-3-yl)-1,2,4-oxadiazole;
[00202] N,N-diethyl-3-(3-(pyridin-3-yl)-1,2,4-oxadiazol-5-yl)benzenesulfonamide;
[00203] 2-fluoro-5-(3-(pyridin-3-yl)-1,2,4-oxadiazol-5-yl)benzonitrile;
[00204] 3-(3-(1H-tetrazol-5-yl)phenyl)-5-(pyridin-3-yl)-1,2,4-oxadiazole;
[00205] 3-(6-chloropyridin-3-yl)-5-(pyridin-3-yl)-1,2,4-oxadiazole;
[00206] 5-(6-chloropyridin-3-yl)-3-(pyridin-3-yl)-1,2,4-oxadiazole;
[00207] 5-(5-(pyridin-3-yl)-1,2,4-oxadiazol-3-yl)pyridin-2(1H)-one;
[00208] 5-(3-(pyridin-3-yl)-1,2,4-oxadiazol-5-yl)pyridin-2(1H)-one;
[00209] N-methyl-3-(3-(pyridin-3-yl)-1,2,4-oxadiazol-5-yl)benzenesulfonamide;
[00210] 3-(3-(pyridin-3-yl)-1,2,4-oxadiazol-5-yl)aniline;
[00211] (3-(3-(pyridin-3-yl)-1,2,4-oxadiazol-5-yl)phenyl)methanamine;
[00212] 5-(2-chloropyridin-4-yl)-3-(pyridin-3-yl)-1,2,4-oxadiazole;

[00213] 4-(3-(pyridin-3-yl)-1,2,4-oxadiazol-5-yl)pyridin-2(lH)-one;
[00214] tert-butyl 3-(3-(pyridin-3-yl)-1,2,4-oxadiazol-5-yl)benzylcarbamate;
[00215] 5-(3-bromophenyl)-3-(pyridin-3-yl)-1,2,4-oxadiazole;
[00216] 1-(3-(3-(pyridin-3-yl)-1,2,4-oxadiazol-5-yl)phenyl)pyrrolidin-2-one;
[00217] tent-butyl 3-(3-(pyridin-3-yl)-1,2,4-oxadiazol-5-yl)phenylcarbamate;
[00218] N,N-dimethyl-l-(3-(3-(pyridin-3-yl)-1,2,4-oxadiazol-5-yl)phenyl)methanamine;
[00219] 5-(3-(piperazin-1-yl)phenyl)-3-(pyridin-3-yl)-1,2,4-oxadiazole;
[00220] 1-(3-(3-(pyridin-3-yl)-1,2,4-oxadiazol-5-yl)phenyl)ethanone;
[00221] 3-(6-chloropyridin-3-yl)-5-(2,3-difluorophenyl)-1,2,4-oxadiazole;
[00222] 3-(6-chloropyridin-3-yl)-5-(3,4-difluorophenyl)-1,2,4-oxadiazole;
[00223] (R)-3-(pyridin-3-yl)-5-(3-(pyrrolidin-2-yl)phenyl)-1,2,4-oxadiazole;
[00224] 5-(3-(1H-pyrazol-3-yl)phenyl)-3-(pyridin-3-yl)-1,2,4-oxadiazole;
[00225] 1-(3-(3-(pyridin-3-yl)-1,2,4-oxadiazol-5-yl)phenyl)ethanol;
[00226] 3-(3-(6-chloropyridin-3-yl)-1,2,4-oxadiazol-5-yl)benzonitrile;
[00227] 3-(4-fluorophenyl)-5-(pyridin-3-yl)-1,2,4-oxadiazole;
[00228] 3-(5-(6-chloropyridin-3-yl)-1,2,4-oxadiazol-3-yl)benzonitrile;
[00229] 3-(5-(2-fluoropyridin-3-yl)-1,2,4-oxadiazol-3-yl)benzonitrile, and [00230] 3-fluoro-5-(5-(pyridin-3-yl)-1,2,4-oxadiazol-3-yl)benzonitrile;
[00231] or pharmaceutically acceptable salts thereof.

[00232] Other specific examples of a4(32 positive allosteric modulators are, for example, 2,5-disubstituted-1,3,4-oxadiazole derivatives, such as:
[00233] 2-(imidazo[1,5-a]pyridin-6-yl)-5-(pyridin-3-yl)-1,3,4-oxadiazole [00234] 2,5-di(pyridin-3-yl)-1,3,4-oxadiazole;
[00235] 2-(5-bromopyridin-3-yl)-5-(pyridin-3-yl)-1,3,4-oxadiazole;
[00236] 2-(pyridin-3-yl)-5-(4-(trifluoromethyl)phenyl)-1,3,4-oxadiazole;
[00237] 2-(pyridin-3-yl)-5-o-tolyl-1,3,4-oxadiazole;
[00238] 2-(pyridin-3-yl)-5-m-tolyl-1,3,4-oxadiazole;
[00239] 2-(pyridin-3-yl)-5 p-tolyl-1,3,4-oxadiazole;
[00240] 2-(5-(pyridin-3-yl)-1,3,4-oxadiazol-2-yl)phenol;
[00241] 3-(5-(pyridin-3-yl)-1,3,4-oxadiazol-2-yl)phenol;
[00242] 4-(5-(pyridin-3-yl)-1,3,4-oxadiazol-2-yl)phenol;
[00243] 2-(3-methoxyphenyl)-5-(pyridin-3-yl)-1,3,4-oxadiazole;
[00244] 2-(4-methoxyphenyl)-5-(pyridin-3-yl)-1,3,4-oxadiazole;

[00245] 2-(2-fluorophenyl)-5-(pyridin-3-yl)-1,3,4-oxadiazole;
[00246] 2-(3-fluorophenyl)-5-(pyridin-3-yl)-1,3,4-oxadiazole;
[00247] 2-(4-fluorophenyl)-5-(pyridin-3-yl)-1,3,4-oxadiazole;
[00248] 2-(2-chlorophenyl)-5-(pyridin-3-yl)-1,3,4-oxadiazole;
[00249] 2-(3-chlorophenyl)-5-(pyridin-3-yl)-1,3,4-oxadiazole;
[00250] 2-(4-chlorophenyl)-5-(pyridin-3-yl)-1,3,4-oxadiazole;
[00251] 2-(2-bromophenyl)-5-(pyridin-3-yl)-1,3,4-oxadiazole;
[00252] 2-(3-bromophenyl)-5-(pyridin-3-yl)-1,3,4-oxadiazole;
[00253] 2-(4-bromophenyl)-5-(pyridin-3-yl)-1,3,4-oxadiazole;
[00254] 3-(5-(pyridin-3-yl)-1,3,4-oxadiazol-2-yl)benzonitrile;
[00255] 4-(5-(pyridin-3-yl)-1,3,4-oxadiazol-2-yl)benzonitrile;
[00256] NN-dimethyl-3-(5-(pyridin-3-yl)-1,3,4-oxadiazol-2-yl)aniline;
[00257] NN-dimethyl-4-(5-(pyridin-3-yl)-1,3,4-oxadiazol-2-yl)aniline;
[00258] 2-(pyridin-3-yl)-5-(3-(trifluoromethyl)phenyl)-1,3,4-oxadiazole;
[00259] 2-(pyridin-3-yl)-5-(3-(trifluoromethoxy)phenyl)-1,3,4-oxadiazole;
[00260] 2-(4-phenoxyphenyl)-5-(pyridin-3-yl)-1,3,4-oxadiazole;
[00261] 2-(4-(benzyloxy)phenyl)-5-(pyridin-3-yl)-1,3,4-oxadiazole;
[00262] 2-(3,4-dimethylphenyl)-5-(pyridin-3-yl)-1,3,4-oxadiazole;
[00263] 2-(3,5-dimethylphenyl)-5-(pyridin-3-yl)-1,3,4-oxadiazole;
[00264] 2-(2,5-dimethylphenyl)-5-(pyridin-3-yl)-1,3,4-oxadiazole;
[00265] 2-(2,4-dimethylphenyl)-5-(pyridin-3-yl)-1,3,4-oxadiazole;
[00266] 2-(3,4-dimethylphenyl)-5-(pyridin-3-yl)-1,3,4-oxadiazole;
[00267] 2-(2,3-dimethoxyphenyl)-5-(pyridin-3-yl)-1,3,4-oxadiazole;
[00268] 2-(2,4-dimethoxyphenyl)-5-(pyridin-3-yl)-1,3,4-oxadiazole;
[00269] 2-(2,5-dimethoxyphenyl)-5-(pyridin-3-yl)-1,3,4-oxadiazole;
[00270] 2-(2,4-dimethoxyphenyl)-5-(pyridin-3-yl)-1,3,4-oxadiazole;
[00271] 2-(3,5-dimethoxyphenyl)-5-(pyridin-3-yl)-1,3,4-oxadiazole;
[00272] 2-(benzo[d][1,3]dioxol-5-yl)-5-(pyridin-3-yl)-1,3,4-oxadiazole;
[00273] 2-(pyridin-3-yl)-5-(3,4,5-trimethoxyphenyl)-1,3,4-oxadiazole;
[00274] 2-(3,4-dichlorophenyl)-5-(pyridin-3-yl)-1,3,4-oxadiazole;
[00275] 2-(2,4-dichlorophenyl)-5-(pyridin-3-yl)-1,3,4-oxadiazole;
[00276] 2-(2,5-dichlorophenyl)-5-(pyridin-3-yl)-1,3,4-oxadiazole;
[00277] 2-(3,4-dichlorophenyl)-5-(pyridin-3-yl)-1,3,4-oxadiazole;

[00278] 5-methyl-2-(5-(pyridin-3-yl)-1,3,4-oxadiazol-2-yl)phenol;
[00279] 2-methyl-5-(5-(pyridin-3-yl)-1,3,4-oxadiazol-2-yl)phenol;
[00280] 2-(3-fluoro-2-methylphenyl)-5-(pyridin-3-yl)-1,3,4-oxadiazole;
[00281] 2-(5-fluoro-2-methylphenyl)-5-(pyridin-3-yl)-1,3,4-oxadiazole;
[00282] 2-(3-fluoro-4-methylphenyl)-5-(pyridin-3-yl)-1,3,4-oxadiazole;
[00283] 2-(2,3-difluorophenyl)-5-(pyridin-3-yl)-1,3,4-oxadiazole;
[00284] 2-(2,4-difluorophenyl)-5-(pyridin-3-yl)-1,3,4-oxadiazole;
[00285] 2-(2,5-difluorophenyl)-5-(pyridin-3-yl)-1,3,4-oxadiazole;
[00286] 2-(3,5-difluorophenyl)-5-(pyridin-3-yl)-1,3,4-oxadiazole;
[00287] 1-(4-(5-(pyridin-3-yl)-1,3,4-oxadiazol-2-yl)phenyl)ethanone;
[00288] 2-(4-isopropylphenyl)-5-(pyridin-3-yl)-1,3,4-oxadiazole;
[00289] 2-(3-methoxy-4-methylphenyl)-5-(pyridin-3-yl)-1,3,4-oxadiazole;
[00290] 2-(4-ethoxyphenyl)-5-(pyridin-3-yl)-1,3,4-oxadiazole;
[00291] 2-(4-(methylthio)phenyl)-5-(pyridin-3-yl)-1,3,4-oxadiazole;
[00292] 2-(3-fluoro-4-methoxyphenyl)-5-(pyridin-3-yl)-1,3,4-oxadiazole;
[00293] 2-(naphthalen-l-yl)-5-(pyridin-3-yl)-1,3,4-oxadiazole;
[00294] 2-(naphthalen-2-yl)-5-(pyridin-3-yl)-1,3,4-oxadiazole;
[00295] 4-chloro-2-(5-(pyridin-3-yl)-1,3,4-oxadiazol-2-yl)phenol;
[00296] 2-(4-tert-butylphenyl)-5-(pyridin-3-yl)-1,3,4-oxadiazole;
[00297] N-(4-(5-(pyridin-3-yl)-1,3,4-oxadiazol-2-yl)phenyl)acetamide;
[00298] 2-(4-propoxyphenyl)-5-(pyridin-3-yl)-1,3,4-oxadiazole;
[00299] 2-(4-isopropoxyphenyl)-5-(pyridin-3-yl)-1,3,4-oxadiazole;
[00300] 2-(5-chloro-2-methoxyphenyl)-5-(pyridin-3-yl)-1,3,4-oxadiazole;
[00301] 2-(4-fluoronaphthalen-l-yl)-5-(pyridin-3-yl)-1,3,4-oxadiazole;
[00302] NN-diethyl-4-(5-(pyridin-3-yl)-1,3,4-oxadiazol-2-yl)aniline;
[00303] 2-(4-butoxyphenyl)-5-(pyridin-3-yl)-1,3,4-oxadiazole;
[00304] 2-(2-methoxy-4-(methylthio)phenyl)-5-(pyridin-3-yl)-1,3,4-oxadiazole;
[00305] 2-(4-(methylsulfonyl)phenyl)-5-(pyridin-3-yl)-1,3,4-oxadiazole;
[00306] 2-(2-chloro-5-(methylthio)phenyl)-5-(pyridin-3-yl)-1,3,4-oxadiazole;
[00307] 2-(2-fluoro-5-(trifluoromethyl)phenyl)-5-(pyridin-3-yl)-1,3,4-oxadiazole;
[00308] 2-(2-chloro-5-(trifluoromethyl)phenyl)-5-(pyridin-3-yl)-1,3,4-oxadiazole;
[00309] 2-(2-phenethylphenyl)-5-(pyridin-3-yl)-1,3,4-oxadiazole;
[00310] 2-(2-bromo-5-methoxyphenyl)-5-(pyridin-3-yl)-1,3,4-oxadiazole;

[00311] 2-(5-bromo-2-chlorophenyl)-5-(pyridin-3-yl)-1,3,4-oxadiazole;
[00312] 2-(2-iodophenyl)-5-(pyridin-3-yl)-1,3,4-oxadiazole;
[00313] 2-(3-iodophenyl)-5-(pyridin-3-yl)-1,3,4-oxadiazole;
[00314] 2-(4-iodophenyl)-5-(pyridin-3-yl)-1,3,4-oxadiazole;
[00315] 2-(pyridin-3-yl)-5-(pyrimidin-5-yl)-1,3,4-oxadiazole;
[00316] 2-(5-methylpyrazin-2-yl)-5-(pyridin-3-yl)-1,3,4-oxadiazole;
[00317] 2-(2-chloro-6-methylpyridin-3-yl)-5-(pyridin-3-yl)-1,3,4-oxadiazole;
[00318] 2-(2-methyl-6-(trifluoromethyl)pyridin-3-yl)-5-(pyridin-3-yl)-1,3,4-oxadiazole;
[00319] 2-(2-(ethylthio)pyridin-3-yl)-5-(pyridin-3-yl)-1,3,4-oxadiazole;
[00320] 2-(2,6-dimethoxypyridin-3-yl)-5-(pyridin-3-yl)-1,3,4-oxadiazole;
[00321] 2-(2-(methylthio)pyridin-3-yl)-5-(pyridin-3-yl)-1,3,4-oxadiazole;
[00322] 5-chloro-3-(5-(pyridin-3-yl)-1,3,4-oxadiazol-2-yl)pyridin-2-ol;
[00323] 2-(2,6-dichloro-5-fluoropyridin-3-yl)-5-(pyridin-3-yl)-1,3,4-oxadiazole;
[00324] 2-(2,5-dichloropyridin-3-yl)-5-(pyridin-3-yl)-1,3,4-oxadiazole;
[00325] 2-(6-chloropyridin-3-yl)-5-(pyridin-3-yl)-1,3,4-oxadiazole;
[00326] 2-(2,6-dichloropyridin-3-yl)-5-(pyridin-3-yl)-1,3,4-oxadiazole;
[00327] 2-(2-chloropyridin-3-yl)-5-(pyridin-3-yl)-1,3,4-oxadiazole;
[00328] 2-(pyridin-3-yl)-5-(quinolin-3-yl)-1,3,4-oxadiazole; and [00329] 2-(2,2-difluorobenzo[d][1,3]dioxol-5-yl)-5-(pyridin-3-yl)-1,3,4-oxadiazole;
[00330] or pharmaceutically acceptable salts thereof.

Novel a402 PAMs of the Present Invention [00331] Various embodiments of the invention described herein include, but are not limited to, pharmaceutically acceptable salts, amides, esters and prodrugs thereof.
[00332] Another embodiment of the invention is a compound of formula (II), wherein Ar 2 and Ara are independently aryl, pyrazinyl, pyridazinyl, pyrimidinyl, triazinyl, or a bicyclic heteroaryl, substituted independently with 0, 1, 2, 3, or 4 substitutents selected from the group consisting of alkoxy, alkoxycarbonyl, alkoxycarbonylamino, alkoxycarbonylaminoalkyl, alkyl, alkylamino, alkylcarbonyl, alkylsulfonyl, amido, amino, aminoalkyl, carboxy, dialkylamino, dialkylaminoalkyl, halo, haloalkyl, haloalkylcarbonyl, heteroaryl, heterocycle, hydroxy, hydroxyalkyl, cyan, nitro, oxo, sulfonamide and dialkylsulfonylformimidamide; provided that when one of Ar 2 and Ar 3 is pyridinyl or aryl, the other is not pyridinyl; when Ar 3 is pyridinyl, Ar 2 is not pyrazinyl.

[00333] Another embodiment of the invention is a compound of formula (II), wherein Ar 2 is optionally substituted phenyl.
[00334] Another embodiment of the invention is a compound of formula (II), wherein Ar 2 is optionally substituted pyridinyl or pyrimidinyl.
[00335] Another embodiment of the invention is a compound of formula (II), wherein Ar 2 is optionally substituted bicyclic heteroaryl.
[00336] Another embodiment of the invention is a compound of formula (II), wherein Ar 3 is optionally substituted pyridinyl, pyrimidinyl or pyridazinyl.
[00337] Another embodiment of the invention is a compound of formula (II), wherein Ar 3 is optionally substituted bicyclic heteroaryl.
[00338] Another embodiment of the invention is a compound of formula (II), wherein Ar 2 and Ara are independently, optionally substituted monocyclic or bicycliic heteroaryl, wherein the monocyclic heteroaryl is six-membered heterocycle, provided that when one of Ar 2 and Ar 3 is pyridinyl, the other is not pyridinyl, and when Ar 3 is pyridinyl, Ar 2 is not pyrazinyl.
[00339] Another embodiment of the invention is a compound of formula (II), wherein Ar 2 and Ara are independently, optionally substituted pyrazinyl, pyridazinyl, pyridinyl, pyrimidinyl, triazinyl, or a bicyclic heteroaryl.
[00340] Another embodiment of the invention is a compound of formula (II), wherein Ar 2 and Ara are independently, optionally substituted phenyl, pyridazinyl, pyridinyl, pyrimidinyl, or bicyclic heteroaryl.
[00341] Another embodiment of the invention is a compound of formula (II), wherein one of Ar 2 and Ar 3 is optionally substituted bicyclic heteroaryl.
[00342] Another embodiment of the invention is a compound of formula (II), wherein one of Ar 2 and Ar 3 is optionally substituted bicyclic heteroaryl selected from the group consisiting of benzimidazolyl, benzo[d][1,3]dioxolyl, benzofuranyl, benzo[d]imidazolyl, benzooxazole, benzo[d][1,2,3]thiadiazolyl, 1,3-benzothiazolyl, benzo[d][1,2,3]triazolyl, 2,2-difluorobenzo[d][1,3]dioxolyl, imidazopyridinyl, indolyl, indazolyl, pyrazolopyrimidinyl, pyrrolopyridinyl, and [1,2,4]triazolopyridinyl.
[00343] Another embodiment of the invention is a compound of formula (II), selected from the group of compounds exemplified in Examples 79-134 described below.
[00344] Another embodiment of the invention is a compound of formula (II), selected from the group of compounds exemplified in Examples 79-105 described below.

[00345] Another embodiment of the invention is a compound of formula (II), selected from the group of compounds exemplified in Examples 79-82, 85-89, 101, and 103-105 described below.
[00346] Another embodiment of the invention is a compound of formula (II), selected from the group of compounds exemplified in Examples 90 and 102 described below.
[00347] Another embodiment of the invention is a compound of formula (II), selected from the group of compounds exemplified in Examples 83-84 and 92-100 described below.
[00348] Another embodiment of the invention is a compound of formula (II), selected from the group of compounds exemplified in Examples 90-91 described below.
[00349] Another embodiment of the invention is a compound of formula (II), selected from the group of compounds exemplified in Examples 106-134 described below.
[00350] Another embodiment of the invention is a compound of formula (II), selected from the group of compounds exemplified in Examples 106-118, 123-131 and 134described below.
[00351] Another embodiment of the invention is a compound of formula (II), selected from the group of compounds exemplified in Examples 119-122 and 132-133 described below.
[00352] Another embodiment of the invention is 5-(2,3-difluorophenyl)-3-(pyrimidin-5-yl)-1,2,4-oxadiazole.
[00353] Another embodiment of the invention is 5-(pyridin-3-yl)-3-(pyrimidin-5-yl)-1,2,4-oxadiazole.
[00354] Another embodiment of the invention is 2-fluoro-N,N-dimethyl-4-(3-(pyrimidin-5-yl)-1,2,4-oxadiazol-5-yl)aniline.
[00355] Another embodiment of the invention is 3-(3-(pyrimidin-5-yl)-1,2,4-oxadiazol-5-yl)benzonitrile.
[00356] Another embodiment of the invention is 5-(3,4-difluorophenyl)-3-(pyridazin-4-yl)-1,2,4-oxadiazole.
[00357] Another embodiment of the invention is 3-(pyridazin-4-yl)-5-(pyridin-3-yl)-1,2,4-oxadiazole.
[00358] Another embodiment of the invention is N,N-dimethyl-N'-(4-(3-(pyrimidin-5-yl)-1,2,4-oxadiazol-5-yl)phenylsulfonyl)-formimidamide.
[00359] Another embodiment of the invention is 5-(4-fluorophenyl)-3-(pyrimidin-5-yl)-1,2,4-oxadiazole.
[00360] Another embodiment of the invention is 5-(3-fluorophenyl)-3-(pyrimidin-5-yl)-1,2,4-oxadiazole.

[00361] Another embodiment of the invention is 3-(pyrimidin-5-yl)-5-(3,4,5-triluorophenyl)-1,2,4-oxadiazole.
[00362] Another embodiment of the invention is 5-(2-chloropyridin-4-yl)-3-(pyrimidin-5-yl)-1,2,4-oxadiazole.
[00363] Another embodiment of the invention is 3-(3-(pyridazin-4-yl)-1,2,4-oxadiazol-5-yl)benzonitrile.
[00364] Another embodiment of the invention is 5-(3-fluorophenyl)-3-(pyridazin-4-yl)-1,2,4-oxadiazole.
[00365] Another embodiment of the invention is 3-(pyridazin-4-yl)-5-(3,4,5-trifluorophenyl)-1,2,4-oxadiazole.
[00366] Another embodiment of the invention is 5-(3,5-difluorophenyl)-3-(pyridazin-4-yl)-1,2,4-oxadiazole.
[00367] Another embodiment of the invention is 5-(4-fluorophenyl)-3-(pyridazin-4-yl)-1,2,4-oxadiazole.
[00368] Another embodiment of the invention is 3-(pyridazin-4-yl)-5-(pyrimidin-5-yl)-1,2,4-oxadiazole.
[00369] Another embodiment of the invention is 3-(pyridazin-4-yl)-5-(2,3,6-trifluorophenyl)- 1,2,4-oxadiazole.
[00370] Another embodiment of the invention is 3-(pyridazin-4-yl)-5-(2,3,4-trifluorophenyl)-1,2,4-oxadiazole.
[00371] Another embodiment of the invention is N,N-dimethyl-N'-(4-(3-(pyridazin-4-yl)-1,2,4-oxadiazol-5-yl)phenylsulfonyl)formimidamide.
[00372] Another embodiment of the invention is 5-(3,4-difluorophenyl)-3-(pyrimidin-5-yl)-1,2,4-oxadiazole.
[00373] Another embodiment of the invention is 3-(3,4-difluorophenyl)-5-(pyrimidin-5-yl)-1,2,4-oxadiazole.
[00374] Another embodiment of the invention is 3-(pyrimidin-5-yl)-5-(2,3,4-trifluorophenyl)- 1,2,4-oxadiazole.
[00375] Another embodiment of the invention is 3-(pyrimidin-5-yl)-5-(2,3,6-trifluorophenyl)-1,2,4-oxadiazole.
[00376] Another embodiment of the invention is 3-(pyrimidin-5-yl)-5-(2,3,4,5-tetrafluorophenyl)-1,2,4-oxadiazole.

[00377] Another embodiment of the invention is 3-(pyrimidin-5-yl)-5-(2,3,6-trifluorophenyl)-1,2,4-oxadiazole.
[00378] Another embodiment of the invention is 3-(pyrimidin-5-yl)-5-(2,3,4,5-tetrafluorophenyl)-1,2,4-oxadiazole.
[00379] Another embodiment of the invention is 5-(imidazo[1,5-a]pyridin-6-yl)-3-(pyridin-3-yl)-1,2,4-oxadiazole.
[00380] Another embodiment of the invention is 5-(1H-indol-6-yl)-3-(pyridin-3-yl)-1,2,4-oxadiazole.
[00381] Another embodiment of the invention is 5-(2,7-dimethylpyrazolo[1,5-a]pyrimidin-6-yl)-3-(pyridin-3-yl)-1,2,4-oxadiazole.
[00382] Another embodiment of the invention is 5-(2,2-difluorobenzo[d][1,3]dioxol-5-yl)-3-(pyridin-3-yl)-1,2,4-oxadiazole.
[00383] Another embodiment of the invention is 5-(2-methylbenzofuran-5-yl)-3-(pyridin-3-yl)-1,2,4-oxadiazole.
[00384] Another embodiment of the invention is 5-(benzo[d][1,2,3]thiadiazol-5-yl)-3-(pyridin-3-yl)-1,2,4-oxadiazole.
[00385] Another embodiment of the invention is 5-(1H-benzo[d]imidazol-5-yl)-3-(pyridin-3-yl)-1,2,4-oxadiazole.
[00386] Another embodiment of the invention is 5-(1H-benzo[d][1,2,3]triazol-5-yl)-3-(pyridin-3-yl)-1,2,4-oxadiazole.
[00387] Another embodiment of the invention is 5-(benzo[d]thiazol-5-yl)-3-(pyridin-3-yl)-1,2,4-oxadiazole.
[00388] Another embodiment of the invention is 3-(pyridin-3-yl)-5-(1H-pyrrolo[2,3-b]pyridin-5-yl)-1,2,4-oxadiazole.
[00389] Another embodiment of the invention is 5-(1H-indol-5-yl)-3-(pyridin-3-yl)-1,2,4-oxadiazole.
[00390] Another embodiment of the invention is 5-(benzofuran-5-yl)-3-(pyridin-3-yl)-1,2,4-oxadiazole.
[00391] Another embodiment of the invention is 5-(1-methyl-lH-benzo[d]imidazol-5-yl)-3-(pyridin-3-yl)-1,2,4-oxadiazole.
[00392] Another embodiment of the invention is 3-(imidazo[1,2-a]pyridin-6-yl)-5-(pyridin-3-yl)-1,2,4-oxadiazole.

[00393] Another embodiment of the invention is 5-(6-chloropyridin-3-yl)-3-(imidazo[1,2-a]pyridin-6-yl)-1,2,4-oxadiazole.
[00394] Another embodiment of the invention is 5-(6-fluropyridin-3-yl)-3-(imidazo[1,2-a]pyridin-6-yl)- 1,2,4-oxadiazole.
[00395] Another embodiment of the invention is 5-(5-fluropyridin-3-yl)-3-(imidazo[1,2-a]pyridin-6-yl)- 1,2,4-oxadiazole.
[00396] Another embodiment of the invention is 5-(1H-indazol-5-yl)-3-(pyridin-3-yl)-1,2,4-oxadiazole.
[00397] Another embodiment of the invention is 5-([1,2,4]triazolo[4,3-a]pyridin-6-yl)-3-(pyridin-3-yl)-1,2,4-oxadiazole.
[00398] Another embodiment of the invention is 5-(3-(pyridin-3-yl)-1,2,4-oxadiazol-5-yl)benzo[d]oxazol-2(3H)-one.
[00399] Another embodiment of the invention is 5-(3-(pyridin-3-yl)-1,2,4-oxadiazol-5-yl)-1 H-benzo [d]imidazole-2(3H)-thione.
[00400] Another embodiment of the invention is 1,3-dimethyl-5-(3-(pyridin-3-yl)-1,2,4-oxadiazol-5-yl)-1 H-benzo [d]imidazol-2(3H)-one.
[00401] Another embodiment of the invention is 6-(3-(pyridin-3-yl)-1,2,4-oxadiazol-5-yl)benzo[d]oxazol-2(3H)-one.
[00402] Another embodiment of the invention is 5-(3-(pyridin-3-yl)-1,2,4-oxadiazol-5-yl)-1 H-benzo [d]imidazol-2(3H)-one.
[00403] Another embodiment of the invention is 6-(3-(Pyridin-3-yl)-1,2,4-oxadiazol-5-yl)benzo[d]oxazol-2-amine.
[00404] Another embodiment of the invention is 6-(3-(pyridin-3-yl)-1,2,4-oxadiazol-5-yl)benzo[d]oxazole.
[00405] Another embodiment of the invention is 5-(5-(pyridin-3-yl)-1,2,4-oxadiazol-3-yl)benzo[d]oxazol-2(3H)-one.
[00406] Another embodiment of the invention is 5-(5-(6-chloropyridin-3-yl)-1,2,4-oxadiazol-3-yl)benzo[d]oxazol-2(3H)-one.
[00407] Another embodiment of the invention is 5-(benzo[d][1,3]dioxol-5-yl)-3-(pyridin-3-yl)-1,2,4-oxadiazole.
[00408] Another embodiment of the invention is 3-(Pyridin-3-yl)-5-(pyrimidin-5-yl)-1,2,4-oxadiazole.

[00409] Another embodiment of the invention is 5-(Pyridazin-4-yl)-3-(pyridin-3-yl)-1,2,4-oxadiazole.
[00410] Another embodiment of the invention is 3-(3,4-difluorophenyl)-5-(pyrimidin-5-yl)-1,2,4-oxadiazole.
[00411 ] Compound names are assigned by using Struct=Name naming algorithm, which is part of the CHEMDRAW ULTRA v. 9Ø7 software suite.
[00412] Geometric isomers can exist in the present compounds. The invention contemplates the various geometric isomers and mixtures thereof resulting from the disposition of substituents around a carbon-carbon double bond, a carbon nitrogen double bond, a cycloalkyl group, or a heterocycloalkyl group. Substituents around a carbon-carbon or carbon-nitrogen double bond are designated as being of Z or E configuration and substituents around a cycloalkyl or heterocycloalkyl are designated as being of cis or trans configuration.
[00413] Within the present invention it is to be understood that compounds disclosed herein can exhibit the phenomenon of tautomerism. Thus, when the formulae drawings within this specification represent one of the possible tautomeric or stereoisomeric forms, it is to be understood that the invention encompasses any tautomeric or stereoisomeric form, and mixtures thereof, and is not to be limited merely to any one tautomeric or stereoisomeric form utilized within the naming of the compounds or formulae drawings.

Preparation of Compounds [00414] Preparation of compounds suitable for the composition of the invention can be understood in connection with the following synthetic schemes and examples, which illustrate a means by which the compounds can be prepared. Methods for preparing suitable nicotinic acetylcholine receptor ligands and suitable nicotinic acetylcholine subtype a4(32 allosteric modulators are readily available in the literature. Suitable compounds can be prepared by conventional methods for chemical synthesis with readily available starting materials. Nicotinic acetylcholine receptor ligands and nicotinic acetylcholine subtype a4(32 allosteric modulators also may be commercially available.
[00415] Abbreviations which have been used in the descriptions of the schemes and the examples that follow are: butyllithium (BuLi), N-(3-dimethylaminopropyl)-N'-ethylcarbodiimide hydrochloride (EDC), ethyl acetate (EtOAc), ethanol (EtOH), hydroxybenzotriazole (HOBT), high-pressure liquid chromatography (HPLC), tetrahydrofuran (THF), triethylamine (NEt3 or Et3N), triphenylphosphine (PPh3), polymer-supported triphenylphosphine (PS-PPh3), methanol (MeOH), dimethylsulfoxide (DMSO), trifluoroacetic acid (TFA), palladium acetate (Pd(OAc)2), acetate (OAc), tris(dibenzylidineacetone) palladium (0) (Pd2(dba)3), 1,l'-Bis(diphenylphosphino)ferrocene]
dichloropalladium(II) (PdC12(dppf)), Dulbecco's Modified Eagle's Medium (DMEM), fetal bovine serum (FBS), N-methyl-D-glucamine (NMDG), and 4-(2-hydroxyethyl)-1-piperazineethanesulfonic acid (HEPES).
[00416] Oxadiazole derivatives suitable for the composition of the invention can be prepared according to conventional methods. Some suitable methods for preparing such oxadiazole derivatives are provided in the Schemes and Examples below.
However, such further illustration is intended only for reference and is not intended in any way to limit the scope of the invention.

Scheme 1 ,OH
I I Ar2N Ar3 Are CI + Ar3 NH2 O-N Jr- (1) (2) (II) [00417] As shown in Scheme 1, compounds of formula (II), wherein Ar 2 and Ar3, are as defined in formula (II) above, can be prepared as described in Scheme 1. Aryl or heteroaryl compounds of general formula (1), can be treated with formula (2) with heat in a solvent including, but not limited to pyridine, to provide of general formula (II).
Scheme 2 O O ///N-N
R3 N,NH2 + R4 OH Rs O R4 H
(4) (5) (6) [00418] As shown in Scheme 2, compounds of formula (4) can be reacted with compounds of formula (5) in POC13 at temperatures from 40-100 C over 1-24 hours to provide compounds of formula (6); wherein R3 is Ari and R4 is Y, or R3 is Y and R4 is Ari.
Alternatively, compounds of formula (4) can be reacted with compounds of formula (5) in the presence of triphenylphosphine, which may optionally be polymer bound, and trichloroacetonitrile in acetonitrile. The mixture may be heated in a microwave oven at 100-175 C for 5-30 minutes as described by Wang, Y.; Sauer, D.R.; Djuric, S.W.
Tetrahedron.
Lett. 2006, 47, 105-108. Another alternative includes combining compounds of formula (4) and compounds of formula (5) in a solvent such as methylene chloride in the presence of 2-chloro-1,3-dimethylimidazolinium chloride and a base such as triethylamine at 15-35 C for 10-120 hours as described by Isobe, T.; Ishikawa, T. J. Org. Chem. 1999, 64, 6989-6992.
Scheme 3 O O O O
Are CI H2N NH2 Are H NH2 (1) (7) (8) O O POCI N-N N-N
H X Y
1 Are X
Ar A H A NH2 s Ar O )CI O
base (8) (9) (I) [00419] As shown in Scheme 3, compounds of formula (1) can be reacted with urea (7) in a solvent such as dichloromethane in the presence of a base such as triethylamine at 25-40 C
for 1-12 hours to provide compounds of formula (8) as described in Sobol, E.;
Bialer, M.;
Yagen, B. J. Med. Chem. 2004, 47, 4316-4326. Alternatively, compounds of formula (1) and (7) may be combined in pyridine at 20-110 C for 1-24 hours to provide compounds of formula (8). Compounds of formula (8) can be treated with POC13 at 25-100 C
for 1-24 hours to provide compounds of formula (9). Compounds of formula (9) can be reacted with H-X-Y in the presence of a base such as lithium bis(trimethylsilyl)amide, sodium bis(trimethylsilyl)amide, potassium bis(trimethylsilyl)amide, potassium t-butoxide, sodium hydride, potassium carbonate, sodium carbonate, cesium or carbonate in a solvent such as tetrahydrofuran, 1-methyl-2-pyrrolidinone, dimethyl sulfoxide, or acetonitrile at temperatures from -20 C to 150 C over 1-48 hours to provide compounds of formula (I).

Scheme 4 ,OH
_Ar I _ Are-. 3 2'I' + a I
ArOH Ar NH2 0-N
(10) (2) (II) [00420] As shown in Scheme 4, compounds of formula (II), wherein Ar 2 and Ara, are as defined in formula (II), can be prepared as described in Scheme 4. Aryl or heteroaryl compounds of general formula (10), can be treated with compounds of formula (2) in the presence of a coupling agent such as N-(3-methylaminopropyl)-N'-ethylcarbodiimide hydrochloride and 1-hydroxybenzotriazole with heat in a solvent including, but not limited to dimethylformamide, to provide compounds of general formula (II).
Scheme 5 OH
Ar3 NH3-H20 3 POC13 Ar3CN NH 2 C02R' ArCONH2 (11) (12) orTFAA/Et3N (13) NOH EDC,HOBT N-O
Ara NH2 Ar3N~Ar2 (2) Ar2CO2H (10) (II) i (COCI)2, or SOCI2 Ar2000I, heat (1) [00421 ] As shown in Scheme 5, compounds of formula (II), wherein Ar 2 and Ara, are as previously defined, can be prepared as described in Scheme 5. Compounds of formula (13), which can be either obtained from a commercial source or prepared from compounds of formula (11). Amides of formula (12) can be prepared by reacting compounds of formula (11) with ammonium hydroxide. Subsequent dehydration of compound of formula (12) with a dehydrating agent, such as but not limited to phosphorous oxychloride or trifluoroacetic anhydride (TFAA)/triethylamine provide compounds of formula (13). Compounds of formula (13) can be reacted with hydroxylamine to give compounds of formula (2).
Compounds of formula (2) react with an aromatic carboxylic acids of formula (10) in the presence of a coupling agent, such as but not limited to N-(3-dimethylaminopropyl)-N'-ethylcarbodiimide hydrochloride (EDC)/1-hydroxybenzotriazole (HOBT), in dimethylformamide at 80-120 C to give compounds of formula (II).
Alternatively, compounds of formula (II) can also be prepared by heating a mixture of compounds of formula (2) and compound of formula (1) which can be obtained from either a commercial source or by the treatment of compound of formula (10) with a chlorinating agent, such as oxalyl chloride or thionyl chloride, in a solvent such as but not limited to pyridine or THE at 60-110 C.

Scheme 6 Are-halo Rm0 ,ORm (17) RmO.B-B.ORm or (Rr')3SnSn(Rn)3 Pd (18) (19) X01) NH2OH NOH Ar3CN N-O
O OH 2) Br2 Br Br 13 Br N Ara + Ar 2-M
base (14) (15) (16) (20) M=Sn(Rn)3, or B(ORm)2 (18) or (19) or s-BuLi Pd B(ORm)3, or (Rn)3SnCl base Pd (21) (22) N-O Pd N-O
M~N Ar3 Are-halo Are%N~'Ar3 (23) (17) (III) M=Sn(Rn)3, or B(ORm)2 [00422] Compounds of formula (III), wherein Ar 2 and Ar3 are as defined in formulas (II), can be prepared as described in Scheme 6. Compounds of formula (14) can be first reacted with hydroxylamine at ambient temperature and then treated with bromine in dichloromethane at temperatures from 0-20 C to provide compounds of formula (15) as described by Berrier, J. V. and Umarvadia, A. S. in EP0979814. The compounds of formula (15) reacts with compounds of formula (13) in the presence a base, such as but not limited to triethylamine, Na2CO3 and K2C03, in a solvent, such as toluene, at temperature ranging 80-110 C over 10-40 hours to provide compounds of formula (16) as described by Humphrey, G. R.; Wright, S. H. B. in J. Heterocyclic Chem. (1989, 26, 23-24). Compounds of formula (17), wherein halo is chloro, bromo or iodo, when treated with an organoboranes of formula (18) or ditin compounds of formulas (19), such as bis(pinacolato)diboron or hexmethlyditin, respectively, wherein Rm or R" are hydrogen, alkyl or aryl, in the presence of a palladium catalyst, such as, but not limited to Pd(OAc)2, PdC12(PPh3)2, Pd(PPh3)4, PdC12(dppf), Pd2(dba)3 provide the corresponding tin or boronic acid or boronic esters of formula (20), wherein M is -Sn-(R")3 or -B(ORm)2. Compounds of formula (16) can be reacted with compounds of formula (20) in the presence of a palladium catalyst, such as, but not limited to Pd(OAc)2, PdC12(PPh3)2, Pd(PPh3)4, PdC12(dppf), Pd2(dba)3, and a base, such as but not limited to CsF, Na2CO3, K2C03 and K3P04, will provide compounds of formula (III).
Alternatively, compounds of formula (16) when treated with organoboranes of formula (18) or ditin compounds of formulas (19), such as bis(pinacolato)diboron or hexmethlyditin, respectively, in the presence of a palladium catalyst provide organotin compounds, organoboronic acids or organoboronic esters compounds of formula (23), wherein M is -Sn-(R")3 or -B(ORm)2. Compounds of formula (23) can also be prepared by the initial treatment of compounds of formula (16) with s-BuLi at temperature ranging from -90 C to -60 C and then reaction with a boronic ester of formula (21) or an organotin compound of formula (22).
Compounds of formula (23) can be reacted with compounds of formula (17) in the presence of a palladium catalyst, such as, but not limited to Pd(OAc)2, PdC12(PPh3)2, Pd(PPh3)4, PdC12(dppf), Pd2(dba)3, and a base, such as but not limited to CsF, Na2CO3, K2C03 and K3P04 to provide compounds of formula (III).

Scheme 7 Ar3CONH2 Ar 2 Me30(BF4) Ar 2 (12) N~NMe2 NH2OH N-O
Ar2CONMe2 Me2N-OMe Ar3~0 HO cA Ar2~N~Ar3 MeO heat (III) (24) (25) (26) [00423] The compounds of formula (III), wherein Ar 2 and Ara are as previously defined can be prepared as shown in Scheme 7. Compounds of formula (24), where in Ar 2 is defined in formulas (II) and (III), when treated with trimethyloxonium tetrafluoroborate in dichloromethane provide compounds of formula (25) as described by McClelland, R. A. in J.
Am. Chem. Soc. (1978, 100, 1844-1849). Compounds of formula (25) can be reacted with compounds of formula (12), wherein Ar 3 is defined in formulas (II) and (III), at temperature ranging from 80-140 C over 1-6 hours to give compounds of formula (26).
Compounds of formula (26) react with hydroxylamine in acetic acid at room temperature to 90 C to provide the compounds of formula (III) as described by Lin, Y.-I., et al., J. Org.
Chem., 44, 4160-4164, 1979.
[00424] In addition, compounds of formula (II) and (III), wherein at least one of Ari and Ar 2 is a N-containing heteroaryl, can be converted to compounds with N+-O- by treatment with an oxidizing agent. Examples of the oxidizing agent include, but are not limited to aqueous hydrogen peroxide and m-chloroperbenzoic acid. The reaction is generally performed in a solvent such as, but not limited to acetonitrile, water, dichloromethane, acetone or a mixture thereof, preferably a mixture of acetonitrile and water, at a temperature from about 0 C to about 80 C, for a period of about 1 hour to about 4 days.

[00425] The compounds and intermediates of the invention may be isolated and purified by methods well-known to those skilled in the art of organic synthesis. Examples of conventional methods for isolating and purifying compounds can include, but are not limited to, chromatography on solid supports such as silica gel, alumina, or silica derivatized with alkylsilane groups, by recrystallization at high or low temperature with an optional pretreatment with activated carbon, thin-layer chromatography, distillation at various pressures, sublimation under vacuum, and trituration, as described for instance in "Vogel's Textbook of Practical Organic Chemistry", 5th edition (1989), by Furniss et al.,, pub.
Longman Scientific & Technical, Essex CM20 2JE, England.
[00426] Compounds and processes suitable for preparing compounds for the composition of the invention will be better understood in connection with the following Examples, which are intended as an illustration of and not a limitation upon the scope of the invention.

Preparation of 2,5-Disubstituted-1,3,4-Oxadiazole Derivatives [00427] Suitable 2,5-disubstituted-1,3,4-oxadiazole derivatives were prepared using readily available starting materials. For example, International Publication WO
02/100826, published December 19, 2002, describes the preparation of some oxadiazole derivatives.
However, Compounds of formula (I) also can be prepared according to the following general methods.
[00428] Method A: A carboxylic acid (0.5 mmol) and an acyl hydrazide (0.5 mmol) were combined in POC13 (2 mL) and stirred at 80-90 C for 2-4 hours. The reaction mixture was then cooled down to ambient temperature and poured into ice water (10-20 g) and basified with saturated aqueous sodium carbonate to pH=8-9. The resultant precipitate was filtered, dried and purified with chromatography on silica gel to provide the corresponding 2,5-disubstituted-1,3,4-oxadiazole. The free base was then dissolved in EtOAc (5-10 mL) and treated with HC1(Aldrich, 4 M in dioxane, 2-3 eq.) at ambient temperature for 5-10 hours.
The precipitate was filtered and dried to provide the corresponding 2,5-disubstituted-1,3,4-oxadiazole hydrochloric acid salt.
[00429] Method B: A Smith Process vial (0.5-2 ml) was charged with a stir bar.
To the vessel were added a carboxylic acid (0.1 mmol), nicotinic hydrazide (Aldrich, 13.7 mg, 0.1 mmol), PS-PPh3 (Fluka, 2.2 mmol/g, 136 mg, 0.3 mmol) and Acetonitrile (anhydrous, Aldrich, 2 mL), followed by CC13CN (Aldrich, 28.8 mg, 0.20 mmol). The reaction vessel was sealed and heated to 150 C for 15 minutes using an EmrysTM Optimizer Microwave (Personal Chemistry, www.personalchemistry.com). After cooling, the reaction vessel was uncapped and the resin was removed by filtration. The mixture was purified by preparative HPLC [Waters, column: Nova-Pak HR C18 6 m 60A Prep-Pak (25mm x 100mm), solvent: acetonitrile/water (v.1 % TFA), 5/95 to 95/5, flow rate of 40 mL/minute. Fractions were collected based upon UV signal threshold, and selected fractions were subsequently analyzed by flow injection analysis mass spectrometry using positive APCI
ionization on a Finnigan LCQ using 70:30 methanol:10 mM NH4OH(aq) at a flow rate of 0.8 ML/minute.].
Some mixtures were purified by an alternative preparative HPLC method [Waters, column:
Sunfire OBD C8 5 m (30 mm x 75 mm); solvent: acetonitrile/l0 mm aqueous ammonium acetate, 10/90 to 100/0; flow rate of 50 mL/minute]. Fractions were collected based upon target mass signal threshold, and selected fractions were subsequently analyzed by flow injection analysis mass spectrometry using the previously described method.

Preparation of 2,5-Disubstituted-1,2,4-Oxadiazole Derivatives [00430] Suitable 2,5-disubstitued-1,2,4-oxadiazole derivatives were prepared using readily available starting materials. For example, International Publication WO
02/100826, published December 19, 2002, describes the preparation of some oxadiazole derivatives.

However, compounds of formula (II) and (III) also can be prepared according to the following general methods.
[00431] Method C: To a solution of an aryl or heteroaryl carboxylic acid (1.0 mmol) in dimethylformamide (anhydrous, 5 mL) was added N-(3-dimethylaminopropyl)-N'-ethylcarbodiimide hydrochloride (EDC) (Aldrich, 192 mg, 1.00 mmol) and 1-hydroxybenzotriazole (HOBt) hydrate (Fluka, 153 mg, 1.00 mmol). The mixture was stirred at ambient temperature for 20 minutes. N'-Hydroxy aryl or heteroaryl carboximidamide (1.0 mmol) was added and the mixture was stirred for 6-10 hours, and then warmed to 140 C for 2-4 hours. The reaction was cooled to ambient temperature and triturated with water (10 mL). The precipitate was filtered and dried under vacuum to give the titled compound.
When the reaction mixture failed to give a precipitate, the reaction mixture was extracted with EtOAc (3 x 30 mL). The combined extracts were concentrated and the residue was purified with chromatography (SiO2, hexane/EtOAc) or preparative HPLC [Waters, column:

XbridgeTM Prep C18 5 m, OBDTM 30 x 100 mm, solvent: acetonitrile/water (pH=lO, prepared with NH4HCO3/NH3=H20) or acetonitrile/water(v/v 0.1% TFA), 5/95 to 95/5, flow rate of 40 mL/minute. Fractions were collected based upon UV signal threshold.].
[00432] Method D: To a solution of N'-Hydroxy aryl or heteroaryl carboximidamide (1.0 mmol) in pyridine (5 mL) was added an aryl or heteroaryl carbonyl chloride (1.0 mmol). The mixture was then stirred at the temperature ranging from 80-100 C for 2-10 hours. The reaction was cooled to ambient temperature and triturated with water (10 mL).
The precipitate was filtered and dried under vacuum to give the titled compound.
When the reaction mixture failed to give a precipitate, the reaction mixture was extracted with EtOAc (3 x 30 mL). The combined extracts were concentrated and the residue was purified with chromatography (SiO2, hexane/EtOAc ) or preparative HPLC [Waters, column:
XbridgeTM
Prep C18 5 m, OBDTM 30 x 100 mm, solvent: acetonitrile/water (PH=10) or acetonitrile/water(v/v 0.1 % TFA), 5/95 to 95/5, flow rate of 40 mL/minute.
Fractions were collected based upon UV signal threshold.].

Example 1 3-(3-(pyridin-3-yl)-1,2,4-oxadiazol-5-yl)benzonitrile [00433] 3-Pyridylamideoxime (Aldrich, 5.5 g, 40 mmol) was dissolved in 60 mL
of pyridine and 3-cyanobenzoyl chloride (Aldrich, 6.6 g, 40 mmol) was added. The reaction mixture was heated to reflux for 4 hours and then cooled to room temperature.
The solution was poured into water (500 mL), filtered, and the solid were collected and dried under vacuum. 1H NMR (300 MHz, CD3OD) 6 ppm 7.87 (td, J=8.0,0.7 Hz, 1 H), 8.10 (dt, J=8.1, 1.4 Hz, 1 H), 8.23 (ddd, J=8.1, 5.6, 0.8 Hz, 1 H), 8.56 (ddd, J=8.0, 1.7, 1.2 Hz, 1 H), 8.64 (td, J=1.7, 0.7 Hz, 1 H), 9.04 (dd, J=5.4, 1.0 Hz, 1 H), 9.23 (dt, J=8.1, 1.7 Hz, 1 H), 9.57 (d, J=1.7 Hz, 1 H); MS (+ESI) m/z 249 (M+H)+.

Example 2 3,5-di(pyridin-3-vi)-1,2,4-oxadiazole [00434] 3-Pyridylamideoxime (5.5 g, 40 mmol) was dissolved in 60 mL of pyridine and nicotinoyl chloride hydrochloride (7.2 g, 40 mmol) was added. The reaction mixture was heated to reflux for 4 hours and then cooled to room temperature. The solution was poured into water (500 mL), basified, filtered, and the solid was collected and dried under vacuum.

1H NMR (300 MHz, DMSO-d6) 6 7.75-7.65 (m, 2H), 8.49-8.45 (m, 1 H), 8.60-8.57 (m, 1 H), 8.84-8.82 (dd, J= 1.7 Hz, 1 H), 8.92-8.90 (dd, J= 1.7 Hz, 1 H), 9.28 (m, 1 H), 9.37 (m, 1 H) ppm; MS (DCI/NH3) m/z 225 (M+H)+.

Example 3 3-(5-(pyridin-3-yl)-1,2,4-oxadiazol-3-yl)benzonitrile Example 3A
3-Cyano-N'-h. doxybenzimidamide [00435] Hydroxylamine (Aldrich, 7.65 g, 100 mmol) in ethanol (100 mL) was treated with 10 N sodium hydroxide (10 mL, 100 mmol). To this solution, isophthalonitrile (Aldrich, 12.8 g, 100 mmol) in 100 mL ethanol was added. The reaction mixture was heated to reflux for 3 hours and then cooled to room temperature. The solvent was removed under vacuum and the residue was purified with flash column chromatography (5%
methanol/dichloromethane) to provide the titled compound. 1H NMR (300 MHz, DMSO-d6) 6 5.98 (bs, 2 H), 7.59 (t, J= 7.4 Hz, 1 H), 8.06-8.0 (m, 2 H), 9.89 (s, 1 H) ppm; MS (DCI/NH3) m/z 162 (M+H)+.
Example 3B
3-(5-(pyridin-3-yl)-1,2,4-oxadiazol-3-yl)benzonitrile [00436] 3-Cyano-N'-hydroxybenzimidamide (0.322 g, 1 mmol) was dissolved in pyridine (10 mL) and nicotinoyl chloride (Aldrich, 0.141 g, 1 mmol) was added. The reaction mixture was heated to reflux for 3 hours and cooled to room temperature. The cooled reaction mixture was quenched with water (25 mL) and filtered. The solid was further purified with flash column chromatography (5% methanol/dichloromethane) to give the titled compound.

iH NMR (300 MHz, DMSO-d6) 6 7.75-7.71 (dd, J= 5.7, 4.1 Hz, 1 H), 7.85 (t, J=
7.8 Hz, 1 H), 8.15-8.12 (d, J= 7.8 Hz, 1 H) 8.44-8.42 (m, 1 H), 8.50 (m, 1 H), 8.60-8.56 (m, 1 H), 8.93-8.91 (dd, J= 1.7 Hz, 1 H), 9.37-9.38 (d, J= 1.7 Hz, 1 H) ppm; MS (DCI/NH3) m/z (M+H)+.

Example 4 3-(5-(6-fluoroyridin-3-yl)-1,2,4-oxadiazol-3-yl)benzonitrile Example 4A
3-Cyano-N'-h, doxybenzimidamide [00437] Hydroxylamine (Aldrich, 7.65 g, 100 mmol) in ethanol (100 mL) was treated with 10 N NaOH (10 mL, 100 mmol). To this solution, isophthalonitrile (Aldrich, 12.8 g, 100 mmol) in 100 mL of ethanol was added. The reaction mixture was heated to reflux for 3 hours and then cooled to room temperature. The solvent was removed under vacuum and the residue was purified with flash column chromatography (5%
methanol/dichloromethane) to give the titled product. 1H NMR (300 MHz, DMSO-d6) 6 5.98 (bs, 2 H), 7.59 (t, J= 7.4 Hz, 1 H), 8.06-8.0 (m, 2 H), 9.89 (s, 1 H) ppm; MS (DCI/NH3) m/z 162 (M+H)+.

Example 4B
3-(5-(6-fluoroyridin-3-yl)-1,2,4-oxadiazol-3-yl)benzonitrile [00438] 3-Cyano-N'-hydroxybenzimidamide (0.322 g, 1 mmol) was dissolved in pyridine 10 mL and 6-fluoronicotinoyl chloride (Frontier Scientific, 0.160 g, 1 mmol) was added. The reaction mixture was heated to reflux for 3 hours and then cooled to room temperature. The cooled reaction mixture was quenched with water (25 mL) and filtered. The solid was further purified with flash column chromatography (5% methanol/dichloromethane) to give the titled product. 1H NMR (300 MHz, DMSO-d6) 6 7.56-7.52 (m, 1 H),7.85 (t, J= 7.9 Hz, 1 H), 8.15-8.12 (m, 1 H), 8.43-8.41 (m, 1 H), 8.49 (m, 1 H), 8.8-8.74 (m, 1 H), 9.11-9.0 (m, 1 H) ppm;
MS (DCI/NH3) m/z 267 (M+H)+.

Example 5 5-(5-bromopyridin-3-yl)-3-(pyridin-3-yl)-1,2,4-oxadiazole [00439] The title compound was prepared according to the procedure of Example 1 using N-hydroxynicotinimidamide (Aldrich) and 5-bromonicotinoyl chloride (Alfa). 1H
NMR (300 MHz, DMSO-d6) 6 8.65-8.69 (m, 1 H), 8.45-8.49 (m,1 H), 8.79 (t, J= 1.7 Hz, 1 H), 8.84 (dd, J=1.7, 2.0 Hz, 1 H), 9.07 (d, J= 2 Hz, 1 H), 9.28-9.29 (m, 1 H), 9.34 (d, J=
1.7 Hz, 1 H) ppm;
MS (DCI/NH3) m/z 303 (M+H)+.

Example 6 3-( yridin-3-yl)-5-(3-(trifluoromethylsulfonyl)phenyl)-1,2,4-oxadiazole Example 6A
3-(trifluoromethylsulfonyl)benzoic acid [00440] A solution of 3-(trifluoromethylthio)benzoic acid (222 mg, 1 mmol) in dichloromethane (10 mL) was stirred with chromium(VI) oxide (Aldrich, 2.0 mmol) at ambient temperature for 12 hours. The title compound was obtained by directly loading the reaction mixture onto a silica gel column and eluting with dichloromethane/methanol (9:1).
iH NMR (300 MHz, DMSO-d6) 6 8.8 (s, 1 H), 8.28 (m, 1 H), 8.05 (m, 1 H), 7.9(m, 1 H) ppm; MS (DCI/NH3) m/z 255 (M+H)+.

Example 6B
3-(trifluoromethylsulfonyl)benzoyl chloride [00441] A solution of the product of Example 6A (198 mg, 0.8 mmol) in dichloromethane (10 mL) was stirred with oxalyl dichloride (Aldrich, 2.0 mmol) and 1 drop of dimethylformamide at ambient temperature for 2 hours. The title compound was obtained by removing the solvent under vacuum as a yellow oil (250 mg) and the compound was used directly in the next step.
Example 6C
3 -( yridin-3-yl)-5-(3-(trifluoromethylsulfonyl)phenyl)-1,2,4-oxadiazole [00442] The title compound was prepared according to the procedure of Example 1 using N-hydroxynicotinimidamide (Aldrich) and the compound of Example 6B. 1H NMR
(300 MHz, DMSO-d6) 6 7.73 (dd, J=4, 5.0 Hz, 1 H), 7.85 (t, J= 8 Hz, 1 H), 8.13 (m,1 H), 8.43 (m, 1 H) 8.5 (m, 1 H), 8.6 (m, 1 H), 8.92 (m, 1 H), 9.37 (m, 1 H) ppm; MS
(DCI/NH3) m/z 356 (M+H)+.
Example 7 3-(3-(6-methylpyridin-3-yl)-1,2,4-oxadiazol-5-yl)benzonitrile Example 7A
N'-hydroxy-6-methylnicotinimidamide [00443] Hydroxylamine (Aldrich, 0.765 g, 10 mmol) in ethanol (10 mL) was treated with a solution 6-methylnicotinonitrile (Aldrich, 12.8 g, 100 mmol) in ethanol (10 ML). The reaction mixture was heated to reflux for 3 hours and then cooled to room temperature. The solvent was removed under vacuum and the residue was purified with flash column chromatography (5% methanol/dichloromethane) give the titled compound. 1H NMR
(300 MHz, DMSO-d6) 6 2.2 (s, 3 H), 6.02 (bs, 2 H), 7.59 (m,l H), 8.06-8.0 (m, 2 H), 10.2 (s, 1 H) ppm; MS (DCI/NH3) m/z 152 (M+H)+.

Example 7B
[00444] 3-(3-(6-methyllpyridin-3-yl)-1,2,4-oxadiazol-5-yl)benzonitrile [00445] The titled compound was prepared according to the procedure of Example 1 using N-hydroxynicotinimidamide (Example 7A) and 3-cyanobenzoyl chloride (Aldrich).

(300 MHz, DMSO-d6) 6 2.59 (s, 3H), 7.52 (d, J=8.1 Hz, 1 H), 7.39 (t, J= 8.5 Hz, 1 H), 8.23-8.21 (m, 1 H), 8.36-8.32 (m, 1 H), 8.53-8.49 (m,l H), 8.64 (m, 1 H), 9.14 (m,l H), ppm; MS
(DCI/NH3) m/z 263 (M+H)+.
Example 8 5-(5-(pyrrol-1-yl)pyridin-3-yl)-3-(pyridin-3-yl)-1,2,4-oxadiazole [00446] To a solution of 5-(1H-pyrrol-1-yl)nicotinic acid (Maybridge, 188 mg, 1.00 mmol) in dimethylformamide (anhydrous, 5 mL) was added N-(3-methylaminopropyl)-N'-ethylcarbodiimide hydrochloride (EDC) (Aldrich, 192 mg, 1.00 mmol) and 1-hydroxybenzotriazole (HOBT) hydrate (Fluka, 153 mg, 1.00 mmol). The mixture was stirred at ambient temperature for 20 minutes. N'-Hydroxynicotinimidamide (137 mg, 1.0 mmol) was added and the mixture was stirred for 6-10 hours, and then warmed to 140 C for 2-4 hours. The reaction was cooled to ambient temperature and triturated with water (10 mL).
The precipitate was filtered and dried under vacuum to give the titled compound. 1H NMR
(300 MHz, DMSO-d6) 6 6.34 - 6.44 (m, 2 H), 7.60 - 7.82 (m, 3 H), 8.50 (dt, J=8.1, 1.9 Hz, 1 H), 8.71 (dd, J=2.5, 1.9 Hz, 1 H), 8.84 (dd, J=4.6, 1.5 Hz, 1 H), 9.21 (d, J=1.7 Hz, 1 H), 9.26 (d, J=2.4 Hz, 1 H), 9.31 (d, J=1.7 Hz, 1 H) ppm; MS (DCI/NH3) m/z 290 (M+H)+.

Example 9 5-(3-(pyridin-3-yl)-1,2,4-oxadiazol-5-yl)pyridin-3-ol [00447] The title compound was prepared according to the procedure of Example 8 using N-hydroxynicotinimidamide (Aldrich) and 5-hydroxynicotinic acid (Matrix Scientific). 1H
NMR (300 MHz, DMSO-d6) 6 7.66 (ddd, J=8.0, 4.9, 1.0 Hz, 1 H), 7.86 (dd, J=2.7, 2.0 Hz, 1 H), 8.31 - 8.55 (m, 2 H), 8.83 (s, 2 H), 9.26 (s, 1 H) ppm; MS (DCI/NH3) m/z 241 (M+H)+.
Example 10 5-(3,4-difluorophenyl)-3-(pyridin-3-yl)-1,2,4-oxadiazole [00456] The title compound was prepared according to the procedure of Example 8 using N-hydroxynicotinimidamide (Aldrich) and 3,4-difluorobenzoic acid (Aldrich). 1H
NMR
(300 MHz, CD3OD) 6 7.52 - 7.67 (m, 2 H), 8.12 (ddd, J=8.7, 4.3, 1.5 Hz, 1 H), 8.19 (ddd, J=10.8, 7.5, 2.0 Hz, 1 H), 8.55 (dt, J=8.1, 1.9 Hz, 1 H), 8.74 (dd, J=5.1, 1.7 Hz, 1 H), 9.29 (dd, J=2.0, 0.7 Hz, 1 H) ppm; MS (DCI/NH3) m/z 260 (M+H)+.

Example 11 5-(2,3-difluorophenyl)-3-(pyridin-3-yl)-1,2,4-oxadiazole [00457] The title compound was prepared according to the procedure of Example 8 using N-hydroxynicotinimidamide (Aldrich) and 2,3-difluorobenzoic acid (Aldrich). 1H
NMR

(300 MHz, CD3OD) 6 7.51 - 7.67 (m, 2 H), 8.08 - 8.14 (m, 1 H), 8.18 (ddd, J=10.7, 7.5, 2.0 Hz, 1 H), 8.55 (dt, J=8.0, 1.9 Hz, 1 H), 8.74 (dd, J=5.2, 1.6 Hz, 1 H), 9.29 (dd, J=2.4, 0.8 Hz, 1 H) ppm; MS (DCI/NH3) m/z 260 (M+H)+.

Example 12 5-(pyrazin-2-yl)-3-(pyridin-3-yl)-1,2,4-oxadiazole [00458] The titled compound was prepared according to the procedure of Example 8 using N-hydroxynicotinimidamide (Aldrich) and pyrazine-2-carboxylic acid (Aldrich).

(300 MHz, CD3OD) 6 7.66 (ddd, J=8.0, 4.9, 1.0 Hz, 1 H), 8.60 (dt, J=8.0, 1.9 Hz, 1 H), 8.77 (dd, J=5.0, 1.8 Hz, 1 H), 8.86 - 8.89 (m, 1 H), 8.89 - 8.91 (m, 1 H), 9.34 (dd, J=2.4, 0.8 Hz, 1 H), 9.56 (d, J=1.6 Hz, 1 H) ppm; MS (DCI/NH3) m/z 226 (M+H)+.

Example 13 5-(3,5-difluorophenyl)-3-(pyridin-3-yl)-1,2,4-oxadiazole [00459] The title compound was prepared according to the procedure of Example 8 using N-hydroxynicotinimidamide (Aldrich) and 3,5-difluorobenzoic acid (Aldrich). 1H
NMR

(300 MHz, CD3OD) 6 7.37 (tt, J=9.0, 2.3 Hz, 1 H), 7.65 (ddd, J=7.9, 5.0, 1.0 Hz, 1 H), 7.82 -7.91 (m, 2 H), 8.56 (dt, J=7.9, 2.0 Hz, 1 H), 8.75 (dd, J=4.8, 1.6 Hz, 1 H), 9.30 (dd, J=2.0, 0.8 Hz, 1 H)ppm; MS (DCI/NH3) m/z 260 (M+H)+.

Example 14 5-(2,3,5-trifluorophenyl)-3-(pyridin-3-yl)-1,2,4-oxadiazole [00460] The title compound was prepared according to the procedure of Example 8 using N-hydroxynicotinimidamide (Aldrich) and 2,3,5-trifluorobenzoic acid (Aldrich).

(300 MHz, CD3OD) 6 7.55 - 7.68 (m, 2 H), 7.83 - 7.90 (m, 1 H), 8.57 (dt, J=8.1, 1.9 Hz, 1 H), 8.75 (dd, J=5.1, 1.7 Hz, 1 H), 9.30 (dd, J=2.2, 0.8 Hz, 1 H) ppm; MS
(DCI/NH3) m/z 278 (M+H)+.

Example 15 5-(2,4,5-trifluorophenyl)-3-(pyridin-3-yl)-1,2,4-oxadiazole [00461 ] The title compound was prepared according to the procedure of Example 8 using N-hydroxynicotinimidamide (Aldrich) and 2,4,5-trifluorobenzoic acid (Aldrich).

(300 MHz, CD3OD) 6 7.55 (td, J=10.3, 6.4 Hz, 1 H), 7.64 (ddd, J=8.1, 5.0, 0.8 Hz, 1 H), 8.23 (ddd, J=10.3, 8.6, 6.4 Hz, 1 H), 8.56 (dt, J=8.1, 1.9 Hz, 1 H), 8.75 (dd, J=5.1, 1.7 Hz, 1 H), 9.29 (d, J=1.4 Hz, 1 H) ppm; MS (DCI/NH3) m/z 278 (M+H)+.

Example 16 5-(2,5-difluorophenyl)-3-(pyridin-3-yl)-1,2,4-oxadiazole [00462] The title compound was prepared according to the procedure of Example 8 using N-hydroxynicotinimidamide (Aldrich) and 2,5-difluorobenzoic acid (Aldrich). 1H
NMR
(300 MHz, CD3OD) 6 7.41 - 7.56 (m, 2 H), 7.65 (ddd, J=7.9, 5.0, 1.0 Hz, 1 H), 7.98 - 8.04 (m, 1 H), 8.57 (dt, J=8.0, 1.9 Hz, 1 H), 8.75 (dd, J=5.2, 1.6 Hz, 1 H), 9.31 (dd, J=2.0, 0.8 Hz, 1 H); MS (DCI/NH3) m/z 260 (M+H)+.

Example 17 5-(4-chloro-2,5-difluorophenyl)-3-(pyridin-3-yl)-1,2,4-oxadiazole [00463] The title compound was prepared according to the procedure of Example 8 using N-hydroxynicotinimidamide (Aldrich) and 4-chloro-2,5-difluorobenzoic acid (Aldrich). 'H
NMR (300 MHz, CD3OD) 6 7.65 (ddd, J=8.0, 4.9, 1.0 Hz, 1 H), 7.73 (dd, J=9.7, 5.9 Hz, 1 H), 8.18 (dd, J=8.8, 6.1 Hz, 1 H), 8.57 (dt, J=8.1, 1.9 Hz, 1 H), 8.75 (dd, J=4.7, 1.7 Hz, 1 H), 9.30 (dd, J=2.2, 0.8 Hz, 1 H) ppm; MS (DCI/NH3) m/z 294 (M+H)+.
Example 18 5-(5-methylpyrazin-2-yl)-3-(pyridin-3-yl)-1,2,4-oxadiazole [00464] The title compound was prepared according to the procedure of Example 8 using N'-hydroxynicotinimidamide (Aldrich) and 5-methylpyrazine-2-carboxylic acid (Aldrich). 1H
NMR (300 MHz, CD3OD) 6 2.71 (s, 3 H), 7.66 (ddd, J=8.0, 5.1, 0.8 Hz, 1 H), 8.59 (dt, J=7.9, 1.8 Hz, 1 H), 8.74 - 8.78 (m, 2 H), 9.33 (dd, J=2.0, 0.8 Hz, 1 H), 9.40 (d, J=1.2 Hz, 1 H) ppm; MS (DCI/NH3) m/z 240 (M+H)+.

Example 19 4-(3-(pyridin-3-yl)-1,2,4-oxadiazol-5-yl)benzonitrile [00465] The title compound was prepared according to the procedure of Example 1 using N'-hydroxynicotinimidamide (Aldrich) and 4-cyanobenzoyl chloride (Aldrich). 1H
NMR
(300 MHz, CD3OD) 6 7.65 (ddd, J=8.0,4.9, 1.0 Hz, 1 H), 8.02 (d, J=8.8 Hz, 2 H), 8.42 (d, J=8.8 Hz, 2 H), 8.57 (dt, J=8.1, 1.9 Hz, 1 H), 8.75 (dd, J=4.7, 1.7 Hz, 1 H), 9.31 (dd, J=2.2, 0.8 Hz, 1 H) ppm; MS (DCI/NH3) m/z 249 (M+H)+.
Example 20 2,3,6-trifluoro-5-(3-(pyridin-3-yl)-1,2,4-oxadiazol-5-yl)phenol [00466] The title compound was prepared according to the procedure of Example 8 using N'-hydroxynicotinimidamide (Aldrich) and 2,4,5-trifluoro-3-hydroxybenzoic acid (Aldrich).
1H NMR (300 MHz, CD3OD) 6 7.63 - 7.76 (m, 2 H), 8.44 (dt, J=7.9, 2.0 Hz, 1 H), 8.83 (dd, J=4.8, 1.6 Hz, 1 H), 9.25 (d, J=1.6 Hz, 1 H) ppm; MS (DCI/NH3) m/z 294 (M+H)+.

Example 21 2-fluoro-5-(3-(pyridin-3-yl)-1,2,4-oxadiazol-5-yl)phenol [00467] The title compound was prepared according to the procedure of Example 8 using N'-hydroxynicotinimidamide (Aldrich) and 4-fluoro-3-hydroxybenzoic acid (Aldrich). 1H
NMR (300 MHz, DMSO-d6) 6 7.45 (dd, J=11.1, 8.7 Hz, 1 H), 7.62 - 7.71 (m, 2 H), 7.78 (dd, J=8.3, 2.0 Hz, 1 H), 8.43 (dt, J=7.9, 1.8 Hz, 1 H), 8.82 (dd, J=5.0, 1.8 Hz, 1 H), 9.24 (d, J=2.0 Hz, 1 H) ppm; MS (DCI/NH3) m/z 258 (M+H)+.

Example 22 2-fluoro-4-(3-(pyridin-3-yl)-1,2,4-oxadiazol-5-yl)phenol [00468] The title compound was prepared according to the procedure of Example 8 using N'-hydroxynicotinimidamide (Aldrich) and 3-fluoro-4-hydroxybenzoic acid (Aldrich). 1H
NMR (300 MHz, CD3OD) 6 7.09 (t, J=8.7 Hz, 1 H), 7.63 (ddd, J=7.9, 5.2, 0.8 Hz, 1 H), 7.86 - 7.93 (m, 2 H), 8.53 (dt, J=7.9, 2.0 Hz, 1 H), 8.72 (dd, J=4.8, 1.6 Hz, 1 H), 9.27 (dd, J=2.4, 0.8 Hz, 1 H) ppm; MS (DCI/NH3) m/z 258 (M+H)+.
Example 23 5-(3-chloro-4-fluorophenyl)-3-(pyridin-3-yl)-1,2,4-oxadiazole [00469] The title compound was prepared according to the procedure of Example 8 using N'-hydroxynicotinimidamide (Aldrich) and 3-chloro-4-fluorobenzoic acid (Aldrich). 1H

NMR (300 MHz, CD3OD) 6 7.54 (t, J=8.8 Hz, 1 H), 7.64 (ddd, J=8.1, 5.0, 0.8 Hz, 1 H), 8.24 (ddd, J=8.6, 4.6, 2.0 Hz, 1 H), 8.39 (dd, J=7.0, 2.2 Hz, 1 H), 8.55 (dt, J=8.1, 1.9 Hz, 1 H), 8.74 (dd, J=4.9, 1.5 Hz, 1 H), 9.29 (dd, J=2.2, 0.8 Hz, 1 H) ppm; MS (DCI/NH3) m/z 276 (M+H)+.

Example 24 5-(3,4-dichlorophenyl)-3-(pyridin-3-yl)-1,2,4-oxadiazole [00470] The title compound was prepared according to the procedure of Example 8 using N'-hydroxynicotinimidamide (Aldrich) and 3,4-dichlorobenzoic acid (Aldrich).

(300 MHz, CD3OD) 6 7.64 (ddd, J=8.0, 5.1, 0.8 Hz, 1 H), 7.82 (d, J=8.3 Hz, 1 H), 8.14 - 8.19 (m, 1 H), 8.40 (d, J=2.0 Hz, 1 H), 8.56 (dt, J=7.9, 2.0 Hz, 1 H), 8.75 (dd, J=5.2, 1.6 Hz, 1 H), 9.29 (dd, J=2.2, 1.0 Hz, 1 H) ppm; MS (DCI/NH3) m/z 292 (M+H)+.
Example 25 2-nitro-5-(3-(pyridin-3-yl)-1,2,4-oxadiazol-5-yl)phenol [00471 ] The title compound was prepared according to the procedure of Example 8 using N-hydroxynicotinimidamide (Aldrich) and 3-hydroxy-4-nitrobenzoic acid (Maybridge). 1H
NMR (300 MHz, DMSO-d6) 6 6.51 (d, J=9.1 Hz, 1 H), 6.92 - 7.31 (s (broad), 1 H), 7.61 (ddd, J=7.9, 4.8, 0.8 Hz, 1 H), 7.68 (dd, J=9.1, 2.4 Hz, 1 H), 8.40 (dt, J=7.9, 2.0 Hz, 1 H), 8.53 (d, J=2.4 Hz, 1 H), 8.77 (dd, J=4.8, 1.6 Hz, 1 H), 9.20 (d, J=1.6 Hz, 1 H) ppm; MS
(DCI/NH3) m/z 285 (M+H)+.

Example 26 5-(2,3,6-trifluorophenyl)-3-(pyridin-3-yl)-1,2,4-oxadiazole [00472] The title compound was prepared according to the procedure of Example 8 using N'-hydroxynicotinimidamide (Aldrich) and 2,3,6-trifluorobenzoic acid (Aldrich). 1H NMR
(300 MHz, CD3OD) 6 7.26 - 7.35 (m, J=9.4, 9.4, 3.8, 2.0 Hz, 1 H), 7.62 - 7.77 (m, 2 H), 8.57 (dt, J=8.0, 1.9 Hz, 1 H), 8.76 (dd, J=4.8, 1.6 Hz, 1 H), 9.30 (dd, J=2.4, 0.8 Hz, 1 H) ppm; MS
(DCI/NH3) m/z 278 (M+H)+.

Example 27 2,2,2-trifluoro-l-(4-(3-(pyridin-3-yl)-1,2,4-oxadiazol-5-yl)phenyl)ethanone trifluoroacetate [00473] The title compound was prepared according to the procedure of Example 8 using N'-hydroxynicotinimidamide (Aldrich) and 4-(2,2,2-trifluoroacetyl)benzoic acid (Aldrich).
iH NMR (300 MHz, CD3OD) 6 7.81 (ddd, J=7.9, 5.2, 0.8 Hz, 1 H), 7.96 (d, J=8.3 Hz, 2 H), 8.28 (d, J=8.7 Hz, 2 H), 8.76 (dt, J=8.2, 1.8 Hz, 1 H), 8.82 (dd, J=5.2, 1.6 Hz, 1 H), 9.38 (d, J=1.6 Hz, 1 H) ppm; MS (DCI/NH3) m/z 320 (M+H)+.

Example 28 5-(3-fluorophenyl)-3-(pyridin-3-yl)-1,2,4-oxadiazole [00474] The title compound was prepared according to the procedure of Example 8 using N'-hydroxynicotinimidamide (Aldrich) and 3-fluorobenzoic acid (Aldrich). 1H
NMR (300 MHz, CD3OD) 6 7.43 - 7.51 (m, J=8.5, 8.5, 2.6, 1.0 Hz, 1 H), 7.61 - 7.72 (m, 2 H), 7.98 (ddd, J=9.1, 2.6, 1.4 Hz, 1 H), 8.08 (ddd, J=8.0,1.3, 1.0 Hz, 1 H), 8.56 (dt, J=8.0,1.9 Hz, 1 H), 8.74 (dd, J=5.2, 1.6 Hz, 1 H), 9.30 (dd, J=2.0, 0.8 Hz, 1 H) ppm; MS (DCI/NH3) m/z 242 (M+H)+.

Example 29 5-(4-fluorophenyl)-3-(pyridin-3-yl)-1,2,4-oxadiazole [00475] The title compound was prepared according to the procedure of Example 8 using N'-hydroxynicotinimidamide (Aldrich) and 4-fluorobenzoic acid (Aldrich). 1H
NMR (300 MHz, CD3OD) 6 7.39 (t, J=8.9 Hz, 2 H), 7.64 (ddd, J=7.9, 4.8, 0.8 Hz, 1 H), 8.27 - 8.35 (m, 2 H), 8.55 (ddd, J=8.1, 2.0, 1.8 Hz, 1 H), 8.74 (dd, J=5.0, 1.8 Hz, 1 H), 9.29 (dd, J=2.2, 1.0 Hz, 1 H) ppm; MS (DCI/NH3) m/z 242 (M+H)+.

Example 30 5-(2-fluorophenyl)-3-(pyridin-3-yl)-1,2,4-oxadiazole [00476] The title compound was prepared according to the procedure of Example 8 using N'-hydroxynicotinimidamide (Aldrich) and 2-fluorobenzoic acid (Aldrich). 1H
NMR (300 MHz, CD3OD) 6 7.38 - 7.49 (m, 2 H), 7.64 (ddd, J=8.0, 4.9, 1.0 Hz, 1 H), 7.70 -7.79 (m, 1 H), 8.28 (td, J=7.5, 1.9 Hz, 1 H), 8.57 (dt, J=8.1, 1.9 Hz, 1 H), 8.74 (dd, J=4.7, 1.7 Hz, 1 H), 9.31 (dd, J=2.2, 0.8 Hz, 1 H) ppm; MS (DCI/NH3) m/z 242 (M+H)+.

Example 31 3-fluoro-5-(3-(pyridin-3-yl)-1,2,4-oxadiazol-5-yl)benzonitrile [00477] The title compound was prepared according to the procedure of Example 8 using N'-hydroxynicotinimidamide (Aldrich) and 3-cyano-5-fluorobenzoic acid (Aldrich). 1H
NMR (300 MHz, CD3OD) 6 7.65 (ddd, J=8.0, 5.1, 0.8 Hz, 1 H), 7.95 (ddd, J=8.1, 2.6, 1.6 Hz, 1 H), 8.32 (ddd, J=8.7, 2.6, 1.4 Hz, 1 H), 8.46 (t, J=1.4 Hz, 1 H), 8.58 (ddd, J=8.1, 2.0, 1.8 Hz, 1 H), 8.76 (dd, J=5.2, 1.6 Hz, 1 H), 9.31 (dd, J=2.0, 0.8 Hz, 1 H) ppm; MS
(DCI/NH3) m/z 267 (M+H)+.

Example 32 3-(2,3-difluorophenyl)-5-(pyridin-3-yl)-1,2,4-oxadiazole hydrochloric acid Example 32A
3-(2,3-difluorophenyl)-5-(pyridin-3-yl)-1,2,4-oxadiazole [00478] The title compound was prepared according to the procedure of Example 1 using 2,3-difluoro-N'-hydroxybenzimidamide (Tyger Scientific) and nicotinoyl chloride hydrochloride (Aldrich). 1H NMR (300 MHz, DMSO-d6) 6 7.42 - 7.61 (m, 1 H), 7.67 - 7.85 (m, 2 H), 7.91 - 8.04 (m, 1 H), 8.57 (dt, J=8.1, 1.9 Hz, 1 H), 8.92 (dd, J=4.8, 1.6 Hz, 1 H), 9.36 (dd, J=2.4, 0.8 Hz, 1 H) ppm; MS (DCI/NH3) m/z 260 (M+H)+.

Example 32B
3-(2,3-difluorophenyl)-5-(pyridin-3-yl)-1,2,4-oxadiazole hydrochloric acid [00479] A solution of the product of Example 32A (320 mg, 1.23 mmol) in ethyl acetate (5 mL) was stirred with hydrochloric acid (Aldrich, 4 M in dioxane, 0.5 mL, 2.0 mmol) at ambient temperature for 4 hours. The titled compound was collected by filtration and dried under vacuum. 1H NMR (300 MHz, CD3OD) 6 7.35 - 7.46 (m, 1 H), 7.49 - 7.63 (m, 1 H), 7.93 - 8.07 (m, 1 H), 8.24 (dd, J=8.1, 5.8 Hz, 1 H), 9.10 (dd, J=5.8, 1.4 Hz, 1 H), 9.23 (dt, J=8.0, 1.8 Hz, 1 H), 9.66 (d, J=2.0 Hz, 1 H) ppm; MS (DCI/NH3) m/z 260 (M+H)+.

Example 33 3-(3,4-difluorophenXl)-5-(pyridin-3-yl)-1,2,4-oxadiazole hydrochloric acid Example 33A
3-(3,4-difluorophenyl)-5-(pyridin-3-yl)-1,2,4-oxadiazole [00480] The title compound was prepared according to the procedure of Example 1 using 3,4-difluoro-N'-hydroxybenzimidamide (Tyger Scientific) and nicotinoyl chloride hydrochloride (Aldrich). 1H NMR (300 MHz, DMSO-d6) 6 7.74 (dd, J=7.5, 4.4 Hz, 1 H), 8.60 (dt, J=7.8, 2.1 Hz, 1 H), 8.93 (dd, J=4.8, 1.6 Hz, 1 H), 9.38 (dd, J=2.2, 1.0 Hz, 1 H), 9.44 - 9.48 (m, 3 H) ppm; MS (DCI/NH3) m/z 260 (M+H)+.
Example 33B
3-(3,4-difluorophenyl)-5-(pyridin-3-yl)-1,2,4-oxadiazole hydrochloric acid [00481] A solution of the product of Example 32A (280 mg, 1.08 mmol) in ethyl acetate (5 mL) was stirred with hydrochloric acid (Aldrich, 4 M in dioxane, 0.5 mL, 2.0 mmol) at ambient temperature for 4 hours. The title compound was collected by filtration and dried under vacuum. 1H NMR (300 MHz, CD3OD) 6 7.52 (td, J=10.5, 8.3 Hz, 1 H), 8.00 -8.17 (m, 2 H), 8.26 (ddd, J=8.1, 5.8, 0.7 Hz, 1 H), 9.08 - 9.14 (m, 1 H), 9.22 -9.30 (m, 1 H), 9.66 (d, J=2.0 Hz, 1 H) ppm; MS (DCI/NH3) m/z 260 (M+H)+.

Example 34 5-(2,6-difluorophenyl)-3-(pyridin-3-yl)-1,2,4-oxadiazole [00482] The title compound was prepared according to the procedure of Example 8 using N'-hydroxynicotinimidamide (Aldrich) and 2,6-difluorobenzoic acid (Aldrich).

(300 MHz, CD3OD) 6 7.29 (t, J=8.6 Hz, 2 H), 7.65 (ddd, J=8.0, 4.9, 1.0 Hz, 1 H), 7.77 (tt, J=8.6, 6.1 Hz, 1 H), 8.57 (ddd, J=8.3, 1.9, 1.7 Hz, 1 H), 8.75 (dd, J=4.7, 1.7 Hz, 1 H), 9.30 (dd, J=2.2, 0.8 Hz, 1 H) ppm; MS (DCI/NH3) m/z 260 (M+H)+.

Example 35 [00483] 3-(3-(pyridin-3-yl)-1,2,4-oxadiazol-5-yl)benzenesulfonamide [00484] The title compound was prepared according to the procedure of Example 8 using N'-hydroxynicotinimidamide (Aldrich) and 3-sulfamoylbenzoic acid (Oakwood). 1H
NMR

(300 MHz, CD3OD) 6 7.65 (ddd, J=8.0, 4.9, 1.0 Hz, 1 H), 7.83 (t, J=7.9 Hz, 1 H), 8.21 (ddd, J=7.9, 1.8, 1.0 Hz, 1 H), 8.45 (dt, J=7.9, 1.4 Hz, 1 H), 8.58 (dt, J=8.0, 1.9 Hz, 1 H), 8.73 -8.77 (m, 2 H), 9.31 (dd, J=2.2, 1.0 Hz, 1 H) ppm; MS (DCI/NH3) m/z 303 (M+H)+.

Example 36 5-(2,4-difluorophenyl)-3-(pyridin-3-yl)-1,2,4-oxadiazole [00485] The title compound was prepared according to the procedure of Example 8 using N'-hydroxynicotinimidamide (Aldrich) and 2,4-difluorobenzoic acid (Aldrich).

(300 MHz, CD3OD) 6 7.23 - 7.36 (m, 2 H), 7.64 (ddd, J=8.1, 5.0, 0.8 Hz, 1 H), 8.35 (td, J=8.5, 6.4 Hz, 1 H), 8.56 (dt, J=7.8, 1.9 Hz, 1 H), 8.74 (dd, J=5.1, 1.7 Hz, 1 H), 9.30 (dd, J=2.2, 0.8 Hz, 1 H) ppm; MS (DCI/NH3) m/z 260 (M+H)+.

Example 37 5-(2,3,4-trifluorophenyl)-3-(pyridin-3-yl)-1,2,4-oxadiazole [00486] The title compound was prepared according to the procedure of Example 8 using N'-hydroxynicotinimidamide (Aldrich) and 2,3,4-trifluorobenzoic acid (Aldrich). 1H NMR
(300 MHz, CD3OD) 6 7.37 - 7.48 (m, J=9.3, 9.3, 7.1, 2.4 Hz, 1 H), 7.64 (ddd, J=7.9, 5.0, 1.0 Hz, 1 H), 8.08 - 8.18 (m, 1 H), 8.56 (dt, J=8.0, 1.9 Hz, 1 H), 8.75 (dd, J=5.0, 1.8 Hz, 1 H), 9.30 (dd, J=2.0, 0.8 Hz, 1 H) ppm; MS (DCI/NH3) m/z 278 (M+H)+.

Example 38 5-(3,4,5-trifluorophenyl)-3-(pyridin-3-yl)-1,2,4-oxadiazole [00487] The title compound was prepared according to the procedure of Example 8 using N'-hydroxynicotinimidamide (Aldrich) and 3,4,5-trifluorobenzoic acid (Aldrich). 1H NMR
(300 MHz, CD3OD) 6 7.64 (ddd, J=8.0, 4.9, 1.0 Hz, 1 H), 8.01 - 8.13 (m, 2 H), 8.56 (ddd, J=8.1, 1.8, 1.6 Hz, 1 H), 8.75 (dd, J=4.8, 1.6 Hz, 1 H), 9.29 (dd, J=2.0, 0.8 Hz, 1 H) ppm; MS
(DCI/NH3) m/z 278 (M+H)+.

Example 39 5-(4-chloro-3-fluorophenyl)-3-(pyridin-3-yi)-1,2,4-oxadiazole [00488] The title compound was prepared according to the procedure of Example 8 using N'-hydroxynicotinimidamide (Aldrich) and 4-chloro-3-fluorobenzoic acid (Aldrich). 'H

NMR (300 MHz, CD3OD) 6 7.64 (ddd, J=7.9, 5.0, 1.0 Hz, 1 H), 7.78 (dd, J=8.3, 7.5 Hz, 1 H), 8.07 (ddd, J=8.3, 2.0, 0.8 Hz, 1 H), 8.12 (dd, J=9.5, 2.0 Hz, 1 H), 8.55 (dt, J=7.9, 2.0 Hz, 1 H), 8.74 (dd, J=5.0, 1.8 Hz, 1 H), 9.28 - 9.30 (m, 1 H) ppm; MS (DCI/NH3) m/z 276 (M+H)+.

Example 40 5-(3-nitrobhenyl)-3-(pyridin-3-yl)-1,2,4-oxadiazole [00489] The title compound was prepared according to the procedure of Example 8 using N-hydroxynicotinimidamide (Aldrich) and 3-nitrobenzoyl chloride (Aldrich). 1H
NMR (300 MHz, CDC13) 6 7.44 - 7.55 (m, 1 H), 7.82 (t, J=8.3 Hz, 1 H), 8.43 - 8.60 (m, 3 H), 8.80 (dd, J=4.7,1.7 Hz, 1 H), 9.07 - 9.13 (m, 1 H), 9.42 (d, J=2.0 Hz, 1 H) ppm; MS
(DCI/NH3) m/z 269 (M+H)+.

Example 41 5-(3-(methylsulfonyl)phenyl)-3-(pyridin-3-yl)-1,2,4-oxadiazole [00490] The title compound was prepared according to the procedure of Example 8 using N'-hydroxynicotinimidamide (Aldrich) and 3-(methylsulfonyl)benzoic acid (Aldrich). 1H
NMR (300 MHz, CD3OD) 6 3.24 (s, 3 H), 7.65 (ddd, J=8.0, 4.9, 1.0 Hz, 1 H), 7.89 - 7.96 (m, 1 H), 8.28 (ddd, J=8.0, 1.9, 1.0 Hz, 1 H), 8.55 - 8.61 (m, 2 H), 8.75 (dd, J=5.1, 1.7 Hz, 1 H), 8.78 (t, J=1.5 Hz, 1 H), 9.32 (dd, J=2.2, 0.8 Hz, 1 H) ppm; MS (DCI/NH3) m/z 302 (M+H)+.
Example 42 3-(2-chloro yridin-4-yl)-5-(pyridin-3-yl)-1,2,4-oxadiazole Example 42A
2-chloro-N'-hydroxyisonicotinimidamide [00491] A solution of 2-chloroisonicotinonitrile (Aldrich, 0.73 g, 5.27 mmol), and hydroxylamine (Aldrich, 50 wt%, 0.348 g, 5.27 mmol) in methanol (10 mL) was heated to reflux and stirred for 1 hour. The volatiles were removed under reduced pressure to give the title compound. 1H NMR (300 MHz, DMSO-d6) 6 6.09 (s, 2 H), 7.67 (dd, J=5.4,1.4 Hz, 1 H), 7.73 (d, J=2.0 Hz, 1 H), 8.40 (d, J=5.2 Hz, 1 H), 10.22 (s, 1 H) ppm; MS
(DCI/NH3) m/z 172 (M+H)+, 174 (M+H)+.

Example 42B
3-(2-chloropyridin-4-yl)-5-(pyridin-3-yl)-1,2,4-oxadiazole [00492] The title compound was prepared according to the procedure of Example 1 using the product of Example 42A and nicotinoyl chloride hydrochloride (Aldrich). 1H
NMR (300 MHz, DMSO-d6) 6 7.73 (ddd, J=8.0, 4.9, 1.0 Hz, 1 H), 8.04 - 8.13 (m, 2 H), 8.59 (dt, J=7.9, 2.0 Hz, 1 H), 8.71 (d, J=5.2 Hz, 1 H), 8.92 (dd, J=5.0, 1.8 Hz, 1 H), 9.38 (dd, J=2.2, 1.0 Hz, 1 H) ppm; MS (DCI/NH3) m/z 259 (M+H)+, 261 (M+H)+.

Example 43 3-(3-(pyridin-3-yl)-1,2,4-oxadiazol-5-yl)benzamide [00493] A solution of the product of Example 1 (248 mg, 1 mmol) in THE (10 ml) was stirred with potassium trimethylsilanolate (257 mg, 2.000 mmol) at 65 C for 10 hours. It was then quenched with water (20 mL) and stirred at ambient temperature for 2 hours. The precipitate was filtered and dried under vacuum to give the title compound. 1H
NMR (300 MHz, DMSO-d6) 6 7.67 (ddd, J=7.9, 4.8, 0.8 Hz, 1 H), 7.78 (t, J=7.7 Hz, 1 H), 8.23 (dt, J=8.1, 1.4, 1.2 Hz, 1 H), 8.30 [s (broad, 2 H], 8.36 (dt, J=8.1, 1.3 Hz, 1 H), 8.48 (dt, J=7.9, 2.0 Hz, 1 H), 8.68 - 8.73 (m, 1 H), 8.83 (dd, J=4.8, 1.6 Hz, 1 H), 9.28 (dd, J=2.2, 1.0 Hz, 1 H) ppm; MS (DCI/NH3) m/z 267 (M+H)+.
Example 44 4-(5-(pyridin-3-yl)-1,2,4-oxadiazol-3-yl)pyridin-2(1H)-one hydrochloric acid [00494] A solution of the product of Example 42 (100 mg, 0.39 mmol) in concentrated hydrochloric acid (Aldrich, 36.5%, 3.0 mL) was heated in an EmryTM Creator microwave to 150 C at 300 watts for 60 minutes. It was then concentrated. The residue was stirred in ethanol/ethyl acetate (v. 1/1, 5 mL) at ambient temperature for 1 hour. The title compound was collected by filtration and dried. 1H NMR (300 MHz, DMSO-d6) b 6.75 (dd, J=6.7, 1.6 Hz, 1 H), 7.04 (d, J=1.6 Hz, 1 H), 7.61 (d, J=5.9 Hz, 1 H), 7.67 - 7.81 (m, 1 H), 8.56 (dt, J=8.0, 1.9 Hz, 1 H), 8.91 (dd, J=5.0, 1.8 Hz, 1 H), 9.31 - 9.40 (m, 1 H) ppm;
MS (DCI/NH3) m/z 241 (M+H)+.

Example 45 tert-blLtyl (3-(pyridin-3-yi)-1,2,4-oxadiazol-5-yl)benzoate [00495] N'-Hydroxynicotinimidamide (274 mg, 2.00 mmol) was coupled with 3-(tert-butoxycarbonyl)benzoic acid (Aldrich) according to the procedure described in Example 8.

1H NMR (300 MHz, CD3OD) 6 1.65 (s, 9 H), 7.65 (ddd, J=7.9, 4.8, 0.8 Hz, 1 H), 7.71 - 7.77 (m, 1 H), 8.26 (ddd, J=7.7, 1.8, 1.6 Hz, 1 H), 8.42 - 8.46 (m, 1 H), 8.57 (dt, J=7.9, 2.0 Hz, 1 H), 8.73 - 8.78 (m, 2 H), 9.31 (dd, J=2.2, 1.0 Hz, 1 H) ppm; MS (DCI/NH3) m/z 324 (M+H)+.

Example 46 2-amino-5-(3-(pyridin-3-yl)-1,2,4-oxadiazol-5-yl)phenol [00496] A solution of the product of Example 25 (284 mg, 1 mmol) in tetrahydrofuran (10 mL) was stirred with Raney -nickel (Aldrich, 100 mg) under hydrogen at ambient temperature for 2 hours. The catalyst was then removed by filtration and the organic solution concentrated to give the title compound. 1H NMR (300 MHz, DMSO-d6) 6 5.67 (s, 2 H), 6.74 (d, J=8.1 Hz, 1 H), 7.41 - 7.50 (m, 2 H), 7.62 (dd, J=8.3, 4.6 Hz, 1 H), 8.39 (dt, J=8.2, 1.9, 1.7 Hz, 1 H), 8.78 (dd, J=4.7, 1.7 Hz, 1 H), 9.20 (d, J=1.7 Hz, 1 H), 9.74 (s (broad), 1 H) ppm; MS (DCI/NH3) m/z 255 (M+H)+.

Example 47 N,N-dimethyl-4-(5-(pyridin-3-yl)-1,2,4-oxadiazol-3 -yl)pyridin-2-amine [00497] A solution of the product of Example 42 (100 mg, 0.39 mmol) in dimethylformamide (2.0 mL) and ammonium hydroxide (0.5 mL) was sealed and heated to 150 C in an EmryTM Creator microwave to 150 C at 300 watts for 60 minutes.
It was then concentrated. The residue was stirred in water (5 mL) at ambient temperature for 1 hour.

The title compound was collected by filtration and dried. 1H NMR (300 MHz, DMSO-d6) 6 3.12 (s, 6 H), 7.18 (dd, J=5.2, 1.2 Hz, 1 H), 7.21 (t, J=1.2 Hz, 1 H), 7.66 -7.78 (m, 1 H), 8.31 (dd, J=4.8, 0.8 Hz, 1 H), 8.57 (ddd, J=8.3, 2.0, 1.6 Hz, 1 H), 8.91 (dd, J=5.0, 1.8 Hz, 1 H), 9.35 (d, J=2.4 Hz, 1 H) ppm; MS (DCI/NH3) m/z 268 (M+H)+.

Example 48 3-(3-(pyridin-3-yl)-1,2,4-oxadiazol-5-yl)benzoic acid [00498] A solution of the product of Example 45 (180 mg, 0.56 mmol) in methylene chloride (5 mL) was stirred with trifluoroacetic acid (1 mL) at room temperature for 4 hours.
It was then concentrated and the residue was stirred in water (15 mL) for 1 hour. The precipitate was collected by filtration and dried to give the title compound.
1H NMR (300 MHz, DMSO-d6) 6 7.66 (ddd, J=7.9, 4.8, 0.8 Hz, 1 H), 7.83 (t, J=7.5 Hz, 1 H), 8.28 (ddd, J=8.0, 1.5, 1.2 Hz, 1 H), 8.42 - 8.51 (m, 2 H), 8.70 (t, J=1.6 Hz, 1 H), 8.83 (dd, J=5.0, 1.8 Hz, 1 H), 9.28 (dd, J=2.2, 1.0 Hz, 1 H) ppm; MS (DCI/NH3) m/z 268 (M+H)+.

Example 49 5-(3-(1H-tetrazol-5-yl)bhenyl)-3-(pyridin-3-yl)-1,2,4-oxadiazole hydrochloric acid [00499] A solution of the product of Example 1 (248 mg, 1.0 mmol) in toluene (anhydrous, mL) was stirred with azidotributylstannane (Aldrich, 498 mg, 1.50 mmol) at 110 C for 15 10 hours. It was then cooled to ambient temperature and stirred with 5 mL of sodium hydroxide (1 N) at ambient temperature for 1 hour. The organic solution was separated, the aqueous mixture was acidified to pH = 2-3 with hydrochloric acid (10 wt.%) and stirred for 2 hours.
The precipitate was collected by filtration and dried to give the title product. 1H NMR (300 MHz, DMSO-d6) 6 7.70-7.76 (m, 1 H), 7.86 - 7.98 (m, 1 H), 8.23 (ddd, J=7.8, 1.7, 1.0 Hz, 0.2 H), 8.38 - 8.46 (m, 1.6 H), 8.49 - 8.53 (m, 0.2 H), 8.56 (ddd, J=8.1, 1.9 Hz, 0.8 H), 8.65 (ddd, J=1.7, 0.7 Hz, 0.2 H), 8.83 - 8.92 (m, 2 H), 9.30 (dd, J=2.2, 0.8 Hz, 0.2 H), 9.33 (dd, J=2.2, 0.8 Hz, 0.8 H) ppm; MS (DCI/NH3) m/z 292 (M+H)+.

Example 50 N,N-diethyl-3-(3-(pyridin-3-yl)-1,2,4-oxadiazol-5-yl)benzenesulfonamide Example 50A
3-(N,N-diethylsulfamoyl)benzoic acid [00500] Diethylamine (Aldrich, 2.5 mL, 24 mmol) was added to a solution of 3-(chlorosulfonyl)benzoic acid (Aldrich, 2.0 g, 9.1 mmol) in anhydrous dichloromethane (20 mL) at 0 C. The mixture was then stirred at 0 C for 2 hours. The volatiles were removed under reduced pressure. The residue was treated with aqueous potassium hydrogensulfate (1 M, 10 mL) and then extracted with ethyl acetate (3x50 mL). The combined extracts were dried over magnesium sulfate filtered and then concentrated to give the title compound. 1H

NMR (300 MHz, CD3OD) 6 1.13 (t, J=7.1 Hz, 6 H), 3.22 - 3.30 (m, 4 H), 7.69 (t, J=7.8 Hz, 1 H), 8.03 (ddd, J=7.9, 1.9, 1.4 Hz, 1 H), 8.25 (dt, J=7.8, 1.4 Hz, 1 H), 8.40 (t, J=1.7 Hz, 1 H) ppm; MS (DCI/NH3) m/z 275 (M+NH4)+

Example 50B
N,N-Diethyl-3-(3-(pyridin-3-yl)-1,2,4-oxadiazol-5-yl)benzenesulfonamide [00501] The title compound was prepared according to the procedure of Example 8 using N'-hydroxynicotinimidamide (Aldrich) and the product of Example 50A. 'H NMR
(300 MHz, CD3OD) 6 1.17 (t, J=7.1 Hz, 6 H), 3.29 - 3.37 (m, 4 H), 7.65 (ddd, J=8.1, 5.0, 0.8 Hz, 1 H), 7.86 (t, J=7.6 Hz, 1 H), 8.14 (ddd, J=7.9, 1.8, 1.2 Hz, 1 H), 8.48 (dt, J=7.9, 1.5 Hz, 1 H), 8.58 (dt, J=8.1, 1.9 Hz, 1 H), 8.62 (t, J=1.5 Hz, 1 H), 8.75 (dd, J=5.1, 1.7 Hz, 1 H), 9.31 (dd, J=2.2, 0.8 Hz, 1 H) ppm; MS (DCI/NH3) m/z 359 (M+H)+.

Example 51 2-fluoro-5-(3-(pyridin-3-yl)-1,2,4-oxadiazol-5-yl)benzonitrile [00502] The title compound was prepared according to the procedure of Example 8 using N'-hydroxynicotinimidamide (Aldrich) and 3-cyano-4-fluorobenzoic acid (Aldrich). 1H
NMR (300 MHz, CD3OD) 6 ppm 7.62 - 7.71 (m, 2 H), 8.54 - 8.63 (m, 2 H), 8.69 (dd, J=5.9, 2.2 Hz, 1 H), 8.75 (dd, J=4.9, 1.5 Hz, 1 H), 9.30 (dd, J=2.0, 1.0 Hz, 1 H); MS
(DCI/NH3) m/z 267 (M+H)+.
Example 52 3-(3-(1H-tetrazol-5-yl)phenyl)-5-(pyridin-3-yl)-1,2,4-oxadiazole hydrochloric acid [00503] The title compound was prepared according to the procedure of Example 49 using the product of Example 3 and azidotributylstannane (Aldrich). 1H NMR (300 MHz, DMSO-d6) 6 7.74 (dd, J=7.3, 5.4 Hz, 1 H), 7.87 (t, J=7.7 Hz, 1 H), 8.25 - 8.43 (m, 2 H), 8.53 - 8.70 (m, 1 H), 8.75 - 8.85 (m, 1 H), 8.88 - 9.00 (m, 1 H), 9.32 - 9.58 (m, 1 H) ppm; MS (DCI/NH3) m/z 292 (M+H)+.
Example 53 3-(6-chloropyridin-3-yl)-5-(pyridin-3-yl)-1,2,4-oxadiazole Example 53A
6-Chloro-N'-hydroxynicotinimidamide [00504] A solution of 2-chloroisonicotinonitrile (Aldrich, 5.0 g, 36.1 mmol) and hydroxylamine (Aldrich, 50% wt, 2.38 g, 36.Ommol) in methanol (100 ml) was heated to reflux and stirred for 1 hour. The volatiles were removed under reduced pressure to give the title compound. 1H NMR (300 MHz, DMSO-d6) 6 6.03 (s, 2 H), 7.54 (d, J=8.7 Hz, 1 H), 8.07 (dd, J=8.3, 2.4 Hz, 1 H), 8.67 (d, J=2.8 Hz, 1 H), 9.94 (s, 1 H) ppm; MS
(DCI/NH3) m/z 172 (M+H)+, 189 (M+H)+.
Example 53B
3-(6-chloropyridin-3-yl)-5-(pyridin-3-yl)-1,2,4-oxadiazole [00505] The title compound was prepared according to the procedure of Example 1 using the product of Example 53A and nicotinoyl chloride hydrochloride (Aldrich). 1H
NMR (300 MHz, CD3OD) 6 7.62 - 7.75 (m, 2 H), 8.53 (dd, J=8.1, 2.4 Hz, 1 H), 8.60 - 8.67 (m, 1 H), 8.85 (dd, J=5.1, 1.7 Hz, 1 H), 9.14 (dd, J=2.4, 0.7 Hz, 1 H), 9.39 (dd, J=2.2, 0.8 Hz, 1 H) ppm; MS (DCI/NH3) m/z 259 (M+H)+, 261 (M+H)+.

Example 54 5-(6-chloropyridin-3-yl)-3-(pyridin-3-yl)-1,2,4-oxadiazole [00506] The title compound was prepared according to the procedure of Example 1 using N'-hydroxynicotinimidamide (Tyger) and 6-chloronicotinoyl chloride (Aldrich).

(300 MHz, CDC13) 6 7.48 (dd, J=7.6, 5.3 Hz, 1 H), 7.57 (d, J=8.5 Hz, 1 H), 8.40 - 8.48 (m, 2 H), 8.80 (dd, J=4.7, 1.7 Hz, 1 H), 9.24 (d, J=2.4 Hz, 1 H), 9.40 (d, J=2.4 Hz, 1 H) ppm; MS
(DCI/NH3) m/z 259 (M+H)+, 261 (M+H)+.

Example 55 5-(5-(pyridin-3-yl)-1,2,4-oxadiazol-3-yl)pyridin-2(1 H)-one [00507] A solution of the product of Example 53B (0.10 g, 0.39 mmol) in concentrated hydrochloric acid (1.0 mL) was heated in a microwave to 150 C at 300 watts for 60 minutes.
It was then concentrated under reduced pressure and the residue was purified by chromatography [silica gel, CHC13/methanol (with 10% v/v ammonium hydroxide), v. 90 /10] to provide the title compound. 1H NMR (300 MHz, DMSO-d6) 6 6.28 - 6.73 (m, 1 H), 7.64 - 7.74 (m, 1 H), 7.98 (dd, J=9.5, 2.7 Hz, 1 H), 8.14 (d, J=2.4 Hz, 1 H), 8.49 - 8.56 (m, 1 H), 8.89 (dd, J=4.7, 1.7 Hz, 1 H), 9.32 (d, J=1.4 Hz, 1 H), 12.17 (s, 1 H) ppm; MS (DCI/NH3) m/z 241 (M+H)+, 258 (M+NH4)+
Example 56 5-(3-(pyridin-3-yl)-1,2,4-oxadiazol-5-yl)pyridin-2(1 H)-one [00508] The title compound was prepared according to the procedure of Example 55 using the product of Example 54. 1H NMR (300 MHz, DMSO-d6) 6 6.55 (d, J=9.2 Hz, 1 H), 7.63 (dd, J=7.6, 5.3 Hz, 1 H), 8.05 (dd, J=9.8, 2.7 Hz, 1 H), 8.31 - 8.47 (m, 2 H), 8.80 (d, J=3.4 Hz, 1 H), 9.22 (s, 1 H), 12.41 (s, 1 H) ppm; MS (DCI/NH3) m/z 241 (M+H)+, 258 (M+NH4)+

Example 57 [00509] N-methyl-3-(3-(pyridin-3-yl)-1,2,4-oxadiazol-5-yl)benzenesulfonamide Example 57A
3-(N-methylsulfamoyl)benzoic acid [00510] The title compound was prepared according to the procedure of Example using 3-(chlorosulfonyl)benzoic acid (Aldrich) and methylamine (Aldrich). 1H
NMR (300 MHz, CD3OD) 6 2.54 (s, 3 H), 7.70 (t, J=7.8 Hz, 1 H), 8.02 - 8.07 (m, 1 H), 8.23 - 8.28 (m, 1 H), 8.45 (t, J=1.9 Hz, 1 H) ppm; MS (DCI/NH3) m/z 233 (M+NH4)+

Example 57B
N-methyl-3-(3-(pyridin-3-yl)-1,2,4-oxadiazol-5-yl)benzenesulfonamide [00511] The title compound was prepared according to the procedure of Example 8 using N'-hydroxynicotinimidamide (Aldrich) and the product of Example 57A. 1H NMR
(300 MHz, CD3OD) 6 2.60 (s, 3 H), 7.65 (ddd, J=8.1, 5.0, 0.8 Hz, 1 H), 7.87 (t, J=8.1 Hz, 1 H), 8.15 (ddd, J=8.0, 1.9, 1.0 Hz, 1 H), 8.48 (ddd, J=7.8, 1.7, 1.0 Hz, 1 H), 8.58 (dt, J=8.1, 1.9 Hz, 1 H), 8.67 (t, J=1.5 Hz, 1 H), 8.75 (dd, J=4.7, 1.7 Hz, 1 H), 9.31 (dd, J=2.2, 0.8 Hz, 1 H) ppm; MS (DCI/NH3) m/z 317 (M+H)+.

Example 58 3-(3-(pyridin-3-yl)-1,2,4-oxadiazol-5-yl)aniline dihydrochloride Example 58A
3-(3-(pyridin-3-yl)-1,2,4-oxadiazol-5-yl)aniline [00512] The title compound was prepared according to the procedure of Example 46 using the product of Example 40. 1H NMR (300 MHz, DMSO-d6) 6 ppm 5.60 (s, 2 H), 6.84 - 6.93 (m, 1 H), 7.24 - 7.33 (m, 2 H), 7.41 (d, J=1.7 Hz, 1 H), 7.60 - 7.67 (m, 1 H), 8.38 - 8.45 (m, 1 H), 8.81 (dd, J=5.1, 1.7 Hz, 1 H), 9.23 (d, J=2.4 Hz, 1 H) ppm; MS (DCI/NH3) m/z 239 (M+H)+, 256 (M+NH4)+

Example 58B
3-(3-(pyridin-3-yl)-1,2,4-oxadiazol-5-yl)aniline dihydrochloride [00513] A solution of the product of Example 58A (60 mg, 0.25 mmol) in ethyl acetate (2 mL) was stirred with hydrochloric acid (Aldrich, 4 M in dioxane, 0.14 mL, 0.55 mmol) at ambient temperature for 4 hours. The title compound was collected by filtration and dried under vacuum. 1H NMR (300 MHz, DMSO-d6) 6 7.44 (d, J=7.9 Hz, 1 H), 7.62 (t, J=7.9 Hz, 1 H), 7.80 (dd, J=7.9, 5.2 Hz, 1 H), 7.85 - 7.96 (m, 2 H), 8.60 (d, J=7.9 Hz, 1 H), 8.90 (d, J=4.8 Hz, 1 H), 9.31 (s, 1 H) ppm; MS (DCI/NH3) m/z 239 (M+H)+, 256 (M+NH4)+

Example 59 (3-(3-(pyridin-3-yl)-1,2,4-oxadiazol-5-yl)phenyl)methanamine bis(hydrochloric acid) [00514] A solution of the product of Example 62 (120 mg, 0.34 mmol) in ethyl acetate (5 mL) was stirred with hydrochloric acid (Aldrich, 4 M in dioxane, 0.5 mL, 2.0 mmol) at ambient temperature for 4 hours. The precipitate was collected by filtration and dried under vacuum to give the title compound. 1H NMR (300 MHz, DMSO-d6) 6 4.21 (q, J=5.9 Hz, 2 H), 7.65 - 7.79 (m, 2 H), 7.86 (dt, J=8.0, 1.3 Hz, 1 H), 8.24 (dt, J=7.7, 1.4 Hz, 1 H), 8.35 -8.45 (m, 3 H), 8.48 (dt, J=8.1, 1.9 Hz, 1 H), 8.85 (dd, J=4.9, 1.2 Hz, 1 H), 9.28 (d, J=1.4 Hz, 1 H) ppm; MS (DCI/NH3) m/z 253 (M+H)+.

Example 60 5-(2-chloropyridin-4-yl)-3-(pyridin-3-yl)-1,2,4-oxadiazole [00515] The title compound was prepared according to the procedure of Example 1 using N-hydroxynicotinimidamide (Aldrich) and 2-chloroisonicotinoyl chloride (Maybridge). 1H
NMR (300 MHz, DMSO-d6) 6 7.68 (ddd, J=7.9, 4.8, 0.8 Hz, 1 H), 8.16 (dd, J=5.1, 1.4 Hz, 1 H), 8.23 (dd, J=1.5, 0.8 Hz, 1 H), 8.42 - 8.54 (m, 1 H), 8.77 (dd, J=5.1, 0.7 Hz, 1 H), 8.84 (dd, J=4.7, 1.7 Hz, 1 H), 9.28 (dd, J=2.2, 0.8 Hz, 1 H) ppm; MS (DCI/NH3) m/z 259 (M+H)+, 261 (M+H)+.
Example 61 4-(3-(pyridin-3-yl)-1,2,4-oxadiazol-5-yl)pyridin-2(1H)-one hydrochloric acid [00516] The title compound was prepared according to the procedure of Example 44 using the product of Example 60. 1H NMR (300 MHz, DMSO-d6) 6 6.81 (dd, J=6.6,1.9 Hz, 1 H), 7.09 - 7.14 (m, 1 H), 7.67 - 7.76 (m, 2 H), 8.51 (dt, J=8.0, 1.9, 1.7 Hz, 1 H), 8.86 (dd, J=4.9, 1.5 Hz, 1 H), 9.28 (d, J=1.4 Hz, 1 H) ppm; MS (DCI/NH3) m/z 241 (M+H)+.

Example 62 tert-butyl 3-(3-(pyridin-3-yi)-1,2,4-oxadiazol-5-yl)benzylcarbamate [00517] The title compound was prepared according to the procedure of Example 8 using N-hydroxynicotinimidamide (Aldrich) and 3-((tert-butoxycarbonylamino)methyl)benzoic acid (Fluka). 1H NMR (300 MHz, DMSO-d6) 6 1.42 (s, 9 H), 4.27 (d, J=6.1 Hz, 2 H), 7.51 -7.72 (m, 4 H), 8.01 - 8.17 (m, 2 H), 8.45 (dt, J=8.1, 1.9 Hz, 1 H), 8.82 (dd, J=5.1, 1.7 Hz, 1 H), 9.26 (dd, J=2.2, 0.8 Hz, 1 H) ppm; MS (DCI/NH3) m/z 353 (M+H)+.

Example 63 5-(3-bromophenyl)-3-(pyridin-3-yl)-1,2,4-oxadiazole [00518] The title compound was prepared according to the procedure of Example 1 using N-hydroxynicotinimidamide (Aldrich) and 3-bromobenzoyl chloride (Aldrich). 1H
NMR
(300 MHz, DMSO-d6) 6 7.60 - 7.71 (m, 2 H), 7.98 (ddd, J=8.1, 2.0, 1.0 Hz, 1 H), 8.22 (ddd, J=7.4, 1.6, 1.3 Hz, 1 H), 8.35 (t, J=1.8 Hz, 1 H), 8.46 (dt, J=7.9, 2.0 Hz, 1 H), 8.83 (dd, J=4.8,1.6 Hz, 1 H), 9.27 (dd, J=2.4, 0.8 Hz, 1 H) ppm; MS (DCI/NH3) m/z 302 (M+H)+, 304 (M+H)+.

Example 64 1-(3-(3-(pyridin-3-yl)-1,2,4-oxadiazol-5-yl)phenyl)pyrrolidin-2-one [00519] A solution of the product of Example 63 (200mg, 0.66 mmol) and pyrrolidin-2-one (Aldrich, 85 mg, 0.99 mmol) in toluene (anhydrous 10 mL) was degassed and purged with nitrogen three times, cesium carbonate (Aldrich, 324 mg, 0.993 mmol) and tris(dibenzylideneacetone)dipalladium(0) (Aldrich, 12.1 mg, 0.013 mmol), 4,5-bis(diphenylphosphino)-9,9-dimethylxanthene (Aldrich, 23.0 mg, 0.040 mmol, xantphos) were added, degassed and purged with nitrogen three times. The mixture was then heated to 100 C and stirred under nitrogen for 15 hours. It was then cooled to ambient temperature and diluted with ethyl acetate (50 mL), washed with brine (2 x 5 mL), concentrated, purified with chromatography (v. ethyl acetate/hexane = 1/1, R=0.1) to give the title compound. 1H
NMR (300 MHz, DMSO-d6) 6 2.04 - 2.19 (m, 2 H), 2.57 (t, J=7.9 Hz, 2 H), 3.95 (t, J=6.9 Hz, 2 H), 7.60 - 7.75 (m, 2 H), 7.84 - 8.10 (m, 2 H), 8.46 (dt, J=7.9, 2.0 Hz, 1 H), 8.64 (t, J=2.0 Hz, 1 H), 8.82 (dd, J=5.0, 1.8 Hz, 1 H), 9.26 (dd, J=2.4, 0.8 Hz, 1 H) ppm; MS
(DCI/NH3) m/z 307 (M+H)+.

Example 65 tert-bglyl (3-(pyridin-3-yi)-1,2,4-oxadiazol-5-yl)phenylcarbamate [00520] The title compound was prepared according to the procedure of Example 8 using N-hydroxynicotinimidamide (Aldrich) and 3-(tent-butoxycarbonylamino)benzoic acid (Aldrich). 'H NMR (300 MHz, CDC13) 6 1.56 (s, 9 H), 6.71 (s, 1 H), 7.39 - 7.58 (m, 2 H), 7.68 (d, J=7.9 Hz, 1 H), 7.89 (d, J=9.1 Hz, 1 H), 8.24 (s, 1 H), 8.45 (d, J=7.9 Hz, 1 H), 8.77 (d, J=4.8 Hz, 1 H), 9.40 (s, 1 H) ppm; MS (DCI/NH3) m/z 339 (M+H)+, 356 (M+NH4)+

Example 66 N,N-dimethyl-l-(3-(3-(Dyridin-3-yl)-1,2,4-oxadiazol-5-yl)phenyl)methanamine, bishydrochloric acid salt [00521] The free base of the title compound was prepared according to the procedure of Example 8 using N'-hydroxynicotinimidamide (Aldrich) and 3-((dimethylamino)methyl)benzoic acid (Aldrich). A solution of this free base in ethyl acetate (5 mL) was treated with hydrochloric acid (Aldrich, 0.5 mL, 4M in dioxane) at ambient temperature for 2 hours. The title compound was collected by filtration and dried under vacuum. iH NMR (300 MHz, CD3OD) 6 2.93 (s, 6 H), 4.51 (s, 2 H), 7.82 (t, J=7.8 Hz, 1 H), 7.90 (dt, J=7.8, 1.5 Hz, 1 H), 8.23 (dd, J=8.0, 5.9 Hz, 1 H), 8.42 (dt, J=7.7, 1.4 Hz, 1 H), 8.49 (t, J=1.5 Hz, 1 H), 9.04 (d, J=5.1 Hz, 1 H), 9.21 (dt, J=8.1, 1.7 Hz, 1 H), 9.56 (s, 1 H) ppm;
MS (DCI/NH3) m/z 281 (M+H)+.
Example 67 5-(3-(piperazin-l-yl)phenyl)-3-(pyridin-3-yl)-1,2,4-oxadiazole bis(hydrochloric acid) Example 67A
tert-butyl4-(3-(3-(pyridin-3-yl)-1,2,4-oxadiazol-5-yl)phenyl)piperazine-l-carboxylate [00522] A solution of the product of Example 63 (200mg, 0.66 mmol) and tert-butyl piperazine-l-carboxylate (Aldrich, 123 mg, 0.66 mmol) in toluene (anhydrous, 10 mL) was degassed and purged with nitrogen three times, sodium t-butoxide (Aldrich, 64 mg, 0.66 mmol) and tris(dibenzylideneacetone)dipalladium(0) (Aldrich, 12.1 mg, 0.013 mmol), 4,5-bis(diphenylphosphino)-9,9-dimethylxanthene (Aldrich, 23.0 mg, 0.040 mmol, xantphos) were added, degassed and purged with nitrogen three times. The mixture was then heated to 100 C and stirred under nitrogen for 15 hours. It was then cooled to ambient temperature and diluted with ethyl acetate (50 mL), washed with brine (2 x 5 mL), concentrated, purified with chromatography (v. ethyl acetate/hexane = 1/1, R f=0.6) to give the title compound. 1H
NMR (300 MHz, CD3OD) 6 1.49 (s, 9 H), 3.25 - 3.30 (m, 4 H), 3.56 - 3.71 (m, 4 H), 7.31 (ddd, J=8.4, 2.6, 0.8 Hz, 1 H), 7.49 (t, J=8.0 Hz, 1 H), 7.64 (ddd, J=8.0, 4.9, 0.7 Hz, 1 H), 7.70 (dt, J=8.0, 1.1 Hz, 1 H), 7.79 (dd, J=2.4, 1.7 Hz, 1 H), 8.56 (dt, J=7.9, 2.0 Hz, 1 H), 8.74 (dd, J=4.9, 1.5 Hz, 1 H), 9.29 (dd, J=2.2, 0.8 Hz, 1 H) ppm; MS (DCI/NH3) m/z 408 (M+H)+.
Example 67B
5-(3-(piperazin-1-Xl)phenXl)-3-(pyridin-3-yl)-1,2,4-oxadiazole bis(hydrochloric acid) [00523] The title compound was prepared according to the procedure of Example 59 using the product of Example 67A. 1H NMR (300 MHz, CD3OD) 6 3.40 - 3.48 (m, 4 H), 3.54 -3.62 (m, 4 H), 7.42 (ddd, J=8.3, 2.8, 0.8 Hz, 1 H), 7.58 (t, J=8.1 Hz, 1 H), 7.78 - 7.94 (m, 2 H), 8.24 - 8.39 (m, 1 H), 9.08 (d, J=5.9 Hz, 1 H), 9.32 (dt, J=8.3, 1.8 Hz, 1 H), 9.59 (d, J=1.6 Hz, 1 H) ppm; MS (DCI/NH3) m/z 308 (M+H)+.

Example 68 1-(3-(3-(pyridin-3-yl)-1,2,4-oxadiazol-5-yl)phenyl)ethanone [00524] The title compound was prepared according to the procedure of Example 8 using N'-hydroxynicotinimidamide (Aldrich) and 3-acetylbenzoic acid (Aldrich). 1H
NMR (300 MHz, CD3OD) 6 2.72 (s, 3 H), 7.66 (ddd, J=8.0, 4.9, 1.0 Hz, 1 H), 7.80 (t, J=8.1 Hz, 1 H), 8.31 (ddd, J=8.1, 1.4, 1.2 Hz, 1 H), 8.47 (ddd, J=8.1, 1.4, 1.2 Hz, 1 H), 8.59 (ddd, J=8.1, 2.0, 1.8 Hz, 1 H), 8.75 (dd, J=5.2, 1.6 Hz, 1 H), 8.81 (t, J=1.4 Hz, 1 H), 9.32 (dd, J=2.4, 0.8 Hz, 1 H) ppm; MS (DCI/NH3) m/z 266 (M+H)+.

Example 69 3-(6-chloropyridin-3-yl)-5-(2,3-difluorophenyl)-1,2,4-oxadiazole [00525] The title compound was prepared according to the procedure of Example 1 using the product of Example 53A and 2,3-difluorobenzoyl chloride (Aldrich). 1H NMR
(300 MHz, DMSO-d6) 6 7.41 - 7.59 (m, 1 H), 7.76 - 7.93 (m, 2 H), 8.05 (dd, J=7.8, 6.1 Hz, 1 H), 8.48 (dd, J=8.3, 2.5 Hz, 1 H), 9.08 (d, J=2.4 Hz, 1 H) ppm; MS (DCI/NH3) m/z 294 (M+H)+, 296 (M+H)+.
Example 70 3-(6-chloropyridin-3-yl)-5-(3,4-difluorophenyl)-1,2,4-oxadiazole [00526] The title compound was prepared according to the procedure of Example 1 using the product of Example 53A and 3,4-difluorobenzoyl chloride (Aldrich). 1H NMR
(300 MHz, DMSO-d6) 6 ppm 7.70 - 7.83 (m, 2 H), 8.03 - 8.15 (m, 1 H), 8.22 - 8.36 (m, 1 H), 8.48 (dd, J=8.1, 2.4 Hz, 1 H), 9.08 (d, J=2.4 Hz, 1 H) ppm; MS (DCI/NH3) m/z 294 (M+H)+, 296 (M+H)+.

Example 71 (R)-3-(pyridin-3-yl)-5-(3-(pyrrolidin-2-yl)phenyl)-1,2,4-oxadiazole bis(hydrochloric acid) Example 71 A
(R)-tert-butte(3-(3-(pyridin-3-yl)-1,2,4-oxadiazol-5-yl)phenyl)pyrrolidine- l -carbox.
[00527] Under nitrogen, to a solution of tert-butyl pyrrolidine-l-carboxylate (Aldrich, 0.52 g, 3.00 mmol) and (-)-sparteine (Aldrich, 0.69 g, 3.0 mmol) in t-butyl methyl ether (Aldrich, anhydrous, 10 mL) was added sec-butyllithium (Aldrich, 1.4 M in cyclohexane, 2.2 mL, 3.1 mmol) at -78 C. After the completion of the addition, it was stirred at -78 C for 3 hours.
Zinc chloride (Aldrich, 1 M in diethyl ether, 2.0 mL, 2.0 mmol) was then added slowly and the resultant solution was stirred at -78 C for additional 30 minutes and then warmed up to ambient temperature, stirred for another 30 minutes at room temperature before the addition of a solution of the product of the Example 63 (0.30 g, 1.0 mmol) in tetrahydrofuran (anhydrous, 5.0 mL) and bis(tri-t-butylphosphine)palladium(0) (Strem, 10.2 mg, 0.02 mmol).
The mixture was stirred at ambient temperature for 15 hours and quenched with ammonium hydroxide (5 mL). The mixture was extracted with ethyl acetate (3 x 20 mL).
The combined extracts were concentrated and purified by chromatography (v. hexanes/ethyl acetate = 1/1, R=0.5) to give the title compound. 1H NMR (300 MHz, CD3OD) 6 1.19 (s (broad), 6 H), 1.48 (s (broad), 3 H), 1.83 - 2.00 (m, 2 H), 2.37 - 2.55 (m, J=8.1, 8.1 Hz, 1 H), 3.57 - 3.72 (m, 2 H), 4.90 - 5.14 (m, 1 H), 7.47 - 7.73 (m, 3 H), 8.00 - 8.25 (m, 2 H), 8.56 (dt, J=8.3, 1.8 Hz, 1 H), 8.74 (dd, J=5.1, 1.7 Hz, 1 H), 9.29 (dd, J=2.0, 0.7 Hz, 1 H) ppm; MS
(DCI/NH3) m/z 393 (M+H)+.
Example 71B
(R)-3-(Pyridin-3-yl)-5-(3-(pyrrolidin-2-yl)phenyl)-1,2,4-oxadiazole bis(hydrochloric acid) [00528] The title compound was prepared according to the procedure of Example 59 using the product of Example 71A. 1H NMR (300 MHz, DMSO-d6) 6 1.98 - 2.27 (m, 3 H), 2.39 -2.50 (m, 1 H), 3.22 - 3.51 (m, 2 H), 4.55 - 4.81 (m, 1 H), 7.71 - 7.82 (m, 2 H), 7.96 (d, J=7.9 Hz, 1 H), 8.26 (dt, J=7.8, 1.2 Hz, 1 H), 8.40 (s, 1 H), 8.58 (dt, J=8.1, 1.9 Hz, 1 H), 8.89 (dd, J=5.2, 1.6 Hz, 1 H), 9.21 - 9.53 (m, J=1.6 Hz, 2 H) ppm; MS (DCI/NH3) m/z 293 (M+H)+.

Example 72 5-(3-(1 H-pyrazol-3-yl)phenyl)-3-(pyridin-3-yl)-1,2,4-oxadiazole [00529] The title compound was prepared according to the procedure of Example 8 using N-hydroxynicotinimidamide (Aldrich) and 3-(1H-pyrazol-3-yl)benzoic acid (Maybridge). 1H
NMR (300 MHz, DMSO-d6) 6 6.91 (d, J=2.4 Hz, 1 H), 7.62 - 7.69 (m, 1 H), 7.72 (t, J=7.8 Hz, 1 H), 7.79 - 7.92 (m, 1 H), 7.82-7.88 (m, 1H), 8.10-8.20 (m, 3 H), 8.49 (dt, J=8.2, 1.9, 1.7 Hz, 1 H), 8.64 (s, 1 H), 8.83 (dd, J=4.7, 1.7 Hz, 1 H), 9.29 (dd, J=2.2, 0.8 Hz, 1 H) ppm; MS
(DCI/NH3) m/z 290 (M+H)+.
Example 73 1-(3-(3-(pyridin-3-yl)-1,2,4-oxadiazol-5-yl)phenyl)ethanol [00530] A solution of the product of Example 68 (265 mg, 1.0 mmol) in ethanol (5 mL) was stirred with sodium borohydride (Aldrich, 83 mg, 2.2 mmol) at room temperature for 16 hours. The inorganic solid was filtered off with a syringe filter and the liquid mixture was purified by preparative HPLC (Gilson, column, Xbridge 5,um, 30 x 100 mm.
eluting solvent, acetonitrile/water (pH = 10, NH4HCO3-NH3=H20 buffer), v. 5/95 to 95/5 over 35 minutes, flow rate, 40 mL/minute, uv, 234 nm). Fractions of the desired product were collected and concentrated to give the desired product. 1H NMR (300 MHz, CD3OD) 6 1.51 (d, J=6.7 Hz, 3 H), 4.97 (q, J=6.6 Hz, 1 H), 7.57 - 7.73 (m, 3 H), 8.13 (dt, J=7.6, 1.5 Hz, 1 H), 8.28 (t, J=1.8 Hz, 1 H), 8.57 (dt, J=7.9, 1.8 Hz, 1 H), 8.74 (dd, J=5.0, 1.8 Hz, 1 H), 9.30 (dd, J=2.2, 1.0 Hz, 1 H) ppm; MS (DCI/NH3) m/z 268 (M+H)+.
Example 74 3-(3-(6-chloropyridin-3-yl)-1,2,4-oxadiazol-5-yl)benzonitrile [00531] The title compound was prepared according to the procedure of Example 1 using the product of Example 53A and 3-cyanobenzoyl chloride (Aldrich). 1H NMR (300 MHz, CDC13) 6 7.52 (d, J=7.8 Hz, 1 H), 7.75 (t, J=8.0 Hz, 1 H), 7.93 (d, J=7.8 Hz, 1 H), 8.30 - 8.50 (m, 2 H), 8.54 (s, 1 H), 9.19 (d, J=2.4 Hz, 1 H) ppm; MS (DCI/NH3) m/z 283 (M+H)+, 300 (M+NH4)+.
Example 75 3-(4-fluorophenyl)-5-(pyridin-3-yl)-1,2,4-oxadiazole [00532] 4-Fluoro-N'-hydroxybenzimidamide (0.154 g, 1 mmol) was dissolved in pyridine (10 mL) and nicotinoyl chloride (Aldrich, 0.141 g, 1 mmol) was added. The reaction mixture was heated to reflux for 3 hours and then cooled to room temperature. The cooled reaction mixture was quenched with water (25 mL) and filtered. The solid was further purified with flash column chromatography (5% methanol/dichloromethane) to give the titled product. 1H
NMR (300 MHz, DMSO-d6) 6 7.47 (t, J= 6.8 Hz, 2 H), 7.74-7.70 (m, 1 H), 8.20-8.15 (m, 2 H), 8.58-8.54 (m, 1 H), 8.91-8.89 (dd, J= 1.7,1.7 Hz, 1 H), 9.35 (d, J= 1.4 Hz, 1 H) ppm; MS
(DCI/NH3) m/z 242 (M+H)+.
Example 76 3-(5-(6-chloropyridin-3-yl)-1,2,4-oxadiazol-3-yl)benzonitrile [00533] The title compound was prepared according to the procedure of Example 4B using 3-cyan-N'-hydroxybenzimidamide (Example 4A) and 6-chloronicotinoyl chloride (Aldrich).

1H NMR (300 MHz, DMSO-d6) 6 7.87 (m, 2 H), 8.17 (m, 1 H), 8.4 (m, 1 H), 8.43 (d, J=1.7 Hz, 1 H), 8.6 (m, 1 H), 9.25 (d, J=1.7 Hz, 1 H) ppm; MS (DCI/NH3) m/z 283 (M+H)+.
Example 77 3-(5-(2-fluoropyridin-3-yl)-1,2,4-oxadiazol-3-yl)benzonitrile [00534] The title compound was prepared according to the procedure of Example 4B using 3-cyan-N'-hydroxybenzimidamide (Example 4A) and 2-fluoronicotinoyl chloride (Aldrich).
iH NMR (300 MHz, DMSO-d6) 6 7.87 (m, 1 H), 7.95 (m, 1 H), 8.17 (m, 1 H), 8.17 (m, 1 H), 8.43 (m, 1 H), 8.6 (m, 1 H), 8.8 (m, 1 H) ppm; MS (DCI/NH3) m/z 267 (M+H)+.

Example 78 3-fluoro-5-(5-(pyridin-3-yl)-1,2,4-oxadiazol-3-yl)benzonitrile [00535] The title compound was prepared according to the procedure of Example 4B using 3-cyan-5-fluoro-N'-hydroxybenzimidamide (Prepared from 5-fluoroisophthalonitrile using the procedure described in Example 4A.) and nicotinoyl chloride (Aldrich). 1H
NMR (300 MHz, DMSO-d6) 6 7.77 (m, 1 H), 8.2 (m, 2 H), 8.4 (m, 1 H), 8.6 (m, 1 H), 8.9 (m, 1 H), 9.4 (m, 1 H) ppm; MS (DCI/NH3) m/z 267 (M+H)+.

Example 79 5-(2,3-Difluorophenyl)-3-(pyrimidin-5-yl)-1,2,4-oxadiazole Example 79A
Pyrimidine-5-carboxamide [00536] A solution of ethyl pyrimidine-5-carboxylate (6.10 g, 40.0 mmol) in methanol (40 mL) was stirred with ammonium hydroxide (4.30 mL, 110 mmol) in a sealed tube at 50 C
for 10 hours. The reaction mixture was then concentrated and the residue was stirred in ethanol/ethyl acetate (v/v 1/4, 50 mL) at ambient temperature for 2 hours. The white precipitate was collected by filtration and dried to give the titled compound.
lH NMR (300 MHz, DMSO-d6) 6 7.85 [s (broad), 1 H], 8.33 [s (broad), 1 H], 9.18 (s, 2 H), 9.32 (s, 1 H) ppm; MS (DCI/NH3) m/z 124 (M+H)+, 141 (M+NH4)+

Example 79B
Pyrimidine-5-carbonitrile [00537] To a suspension of the product of Example 79A (6.75 g, 54.8 mmol) and triethylamine (Aldrich, 15.28 mL, 110 mmol) in anhydrous CH2C12 (Aldrich, 400 mL) was slowly added a solution of trifluoroacetic anhydride (Aldrich, 9.30 mL, 65.8 mmol) in anhydrous CH2C12 (100 mL) at 0-10 C over 1 hours. It was then stirred at 0 C
to ambient temperature for 2 hours. The reaction was then quenched with water (10 mL) and washed with NaOH (1 N, 20 mL) and brine (2 x 10 mL) before being concentrated under reduced pressure at less than 30 C to supply the titled compound. 1H NMR (300 MHz, DMSO-d6) 6 9.34 (s, 2 H), 9.47 (s, 1 H) ppm; MS (DCI/NH3) m/z 123 (M+NH4)+

Example 79C
N'-H. doxypyrimidine-5-carboximidamide [00538] A solution of the product of Example 79B (5.47 g, 52 mmol) and aqueous hydroxylamine (Aldrich, 50%, 2.3 mL, 78 mmol) in methanol (50 mL) was stirred at 65 C
for 1 hour and then concentrated under reduced pressure to remove the volatiles. The residue was triturated with EtOAc (30 mL). The precipitates were collected by filtration and dried to give the titled compound. 1H NMR (300 MHz, DMSO-d6) 6 6.14 (s, 2 H), 9.03 (s, 2 H), 9.18 (s, 1 H), 10.06 (s, 1 H) ppm; MS (DCI/NH3) m/z 139 (M+H)+, 156 (M+NH4)+

Example 79D
5-(2,3-Difluorophenyl)-3-(pyrimidin-5-yl)-1,2,4-oxadiazole [00539] The titled compound was prepared according to the procedure of Method D using the product of Example 79C and 2,3-difluorobenzoyl chloride (Aldrich). 1H NMR
(300 MHz, CDC13) 6 7.27 - 7.39 (m, 1 H), 7.42 - 7.56 (m, 1 H), 7.95 - 8.08 (m, 1 H), 9.39 (s, 1 H), 9.49 (s, 2 H) ppm; MS (DCI/NH3) m/z 261 (M+H)+.

Example 80 5-(pyridin-3-yl)-3-(pyrimidin-5-yl)-1,2,4-oxadiazole [00540] The titled compound was prepared according to the procedure of Method D using the product of Example 79C and nicotinoyl chloride hydrochloride (Aldrich). 1H
NMR (300 MHz, DMSO-d6) 6 7.74 (ddd, J=8.0, 4.9, 0.7 Hz, 1 H), 8.54 - 8.64 (m, 1 H), 8.93 (dd, J=4.9, 1.5 Hz, 1 H), 9.39 (d, J=1.7 Hz, 1 H), 9.45 (s, 1 H), 9.46 (s, 2 H) ppm; MS
(DCI/NH3) m/z 226 (M+H)+.
Example 81 2-fluoro-N,N-dimethyl-4-(3-(pyrimidin-5-yl)-1,2,4-oxadiazol-5-yl)aniline [00541] The titled compound was prepared according to the procedure of Method C using the product of Example 79C and 3,4-difluorobenzoic acid (Aldrich). 1H NMR (300 MHz, CDC13) 6 3.06 (s, 3 H), 3.07 (s, 3 H), 6.89 (t, J=8.7 Hz, 1 H), 7.76 - 7.96 (m, 2 H), 9.36 (s, 1 H), 9.43 - 9.47 (m, 2 H) ppm; MS (DCI/NH3) m/z 286 (M+H)+.

Example 82 3-(3-(pyrimidin-5-yl)-1,2,4-oxadiazol-5-yl)benzonitrile [00542] The titled compound was prepared according to the procedure of Method D using the product of Example 79C and 3-cyanobenzoyl chloride (Aldrich). 1H NMR (300 MHz, DMSO-d6) 6 7.91 (t, J=7.9 Hz, 1 H), 8.24 (dt, J=7.7, 1.4, 1.2 Hz, 1 H), 8.53 (dt, J=8.1, 1.4, 1.2 Hz, 1 H), 8.67 (t, J=1.8 Hz, 1 H), 9.45 (s, 1 H), 9.46 (s, 2 H) ppm; MS
(DCI/NH3) m/z 250 (M+H)+.

Example 83 5-(3,4-Difluorophenyl)-3-(pyridazin-4-yl)-1,2,4-oxadiazole hydrochloride Example 83A
Methyl pyridazine-4-carbox.
[00543] To a solution of pyridazine-4-carboxylic acid (Aldrich, 5.0 g, 40.3 mmol) in methanol (anhydrous, Aldrich, 100 mL) was added sulfuric acid (concentrated, Aldrich, 2 mL). The reaction mixture was then heated to reflux and stirred for 16 hours.
The volatiles were removed under reduced pressure. The residue was basified to pH= 8-9 with saturated sodium carbonate (20 mL) and then extracted with EtOAc (3 x 100 mL). The combined extracts were washed with brine (2 x 20 mL) and dried over magnesium sulfate.
The drying agent was filtered off and the organic solution was concentrated and dried to give the titled compound. 1H NMR (300 MHz, DMSO-d6) 6 3.34 (s, 3 H), 8.10 (dd, J=5.2, 2.4 Hz, 1 H), 9.52 (dd, J=5.2,1.2 Hz, 1 H), 9.58 (dd, J=2.2,1.4 Hz, 1 H) ppm; MS (DCI/NH3) m/z 139 (M+H)+.

Example 83B
pyridazine-4-carboxamide [00544] The title compound was prepared according to the procedure of Example using the product of Example 83A and ammonium hydroxide (Aldrich). 1H NMR (300 MHz, DMSO-d6) 6 8.00 (dd, J=5.2, 2.4 Hz, 1 H), 8.46 [s (broad), 2 H], 9.44 (dd, J=5.2, 1.2 Hz, 1 H), 9.55 (dd, J=2.2, 1.4 Hz, 1 H) ppm; MS (DCI/NH3) m/z 124 (M+H)+, 141 (M+NH4)+

Example 83C
pyridazine-4-carbonitrile [00545] The title compound was prepared according to the procedure of Example using the product of Example 83B and trifluoroacetic anhydride (Aldrich). 1H
NMR (300 MHz, DMSO-d6) 6 8.28 (dd, J=5.4, 2.2 Hz, 1 H), 9.57 (dd, J=5.2, 1.2 Hz, 1 H), 9.66 (dd, J=2.2, 1.4 Hz, 1 H) ppm; MS (DCI/NH3) m/z 123 (M+NH4)+
Example 83D
N'-Hydroxypyridazine-4-carboximidamide [00546] The title compound was prepared according to the procedure of Example using the product of Example 83C and hydroxylamine (Aldrich). 1H NMR (300 MHz, DMSO-d6) 6 6.19 (s, 2 H), 7.87 (dd, J=5.4, 2.4 Hz, 1 H), 9.25 (dd, J=5.4, 1.4 Hz, 1 H), 9.46 (dd, J=2.4, 1.4 Hz, 1 H), 10.36 (s, 1 H) ppm; MS (DCI/NH3) m/z 139 (M+H)+, 156 (M+NH4)+

Example 83E
5-(3,4-difluorophenyl)-3-(pyridazin-4-yl)-1,2,4-oxadiazole [00547] The titled compound was prepared according to the procedure of Method D using the product of Example 83D and 3,4-difluorobenzoyl chloride (Aldrich). 1H NMR
(300 MHz, DMSO-d6) 6 7.80 (dt, J=10.5, 8.3 Hz, 1 H), 8.08 - 8.19 (m, 1 H), 8.29 (dd, J=5.4, 2.4 Hz, 1 H), 8.31 - 8.38 (m, 1 H), 9.55 (dd, J=5.4, 1.4 Hz, 1 H), 9.82 (dd, J=2.2, 1.2 Hz, 1 H) ppm; MS (DCI/NH3) m/z 261 (M+H)+.

Example 83F
5-(3,4-Difluorophenyl)-3-(pyridazin-4-yl)-1,2,4-oxadiazole hydrochloride [00548] A solution of the product of Example 83E (140 mg, 0.54 mmol) in ethyl acetate (5.0 mL) was stirred with hydrochloric acid (Aldrich, 4 M in dioxane, 0.25 mL, 1.0 mmol) at ambient temperature for 10 hours. The white precipitates were collected by filtration and dried to give the title compound. 1H NMR (300 MHz, DMSO-d6) 6 7.81 (dt, J=10.5, 8.5 Hz, 1 H), 8.08 - 8.19 (m, 1 H), 8.26 - 8.39 (m, 2 H), 9.55 (dd, J=5.4, 1.4 Hz, 1 H), 9.83 (dd, J=2.4, 1.4 Hz, 1 H) ppm; MS (DCI/NH3) m/z 261 (M+H)+.

Example 84 3-(Pyridazin-4-yl)-5-(pyridin-3-yl)-1,2,4-oxadiazole [00549] The titled compound was prepared according to the procedure of Method D using the product of Example 83D and nicotinoyl chloride hydrochloride (Aldrich). 1H
NMR (300 MHz, DMSO-d6) 6 7.75 (ddd, J=8.0, 4.9, 0.7 Hz, 1 H), 8.32 (dd, J=5.3, 2.2 Hz, 1 H), 8.61 (dt, J=8.2, 1.8 Hz, 1 H), 8.93 (dd, J=5.1, 1.7 Hz, 1 H), 9.39 (dd, J=2.2, 0.8 Hz, 1 H), 9.55 (dd, J=5.4,1.4 Hz, 1 H), 9.85 (dd, J=2.2,1.2 Hz, 1 H) ppm; MS (DCI/NH3) m/z 226 (M+H)+.

Example 85 N,N-Dimeth (4-(3-(pyrimidin-5-XI)-1,2,4-oxadiazol-5-XI)bhenylsulfonXl)formimidamide [00550] The titled compound was prepared according to the procedure of Method D using the product of Example 79C and 4-sulfamidobenzoyl chloride dimethylformamide complex (Alfa Aesar). 1H NMR (300 MHz, DMSO-d6) 6 2.94 (s, 3 H), 3.18 (s, 3 H), 8.06 (d, J=8.8 Hz, 2 H), 8.30 (s, 1 H), 8.37 (d, J=8.8 Hz, 2 H), 9.45 (s, 1H), 9.46 (s, 2 H) ppm; MS
(DCI/NH3) m/z= 359 (M+H)+, 376 (M+NH4)+

Example 86 5-(4-fluorophenyl)-3-(pyrimidin-5-yl)-1,2,4-oxadiazole [00551] The titled compound was prepared according to the procedure of Method D using the product of Example 79C and 4-fluorobenzoyl chloride (Aldrich). 1H NMR (300 MHz, DMSO-d6) 6 7.51 - 7.59 (m, 2 H), 8.27 - 8.34 (m, 2 H), 9.44 (s, 2 H), 9.45 (S, 1H) ppm; MS
(DCI/NH3) m/z= 243 (M+H)+, 260 (M+NH4)+

Example 87 5-(3-fluorobhenyl)-3-(pyrimidin-5-yl)-1,2,4-oxadiazole [00552] The titled compound was prepared according to the procedure of Method D using the product of Example 79C and 3-fluorobenzoyl chloride (Aldrich). 1H NMR (300 MHz, DMSO-d6) 6 7.61 - 7.71 (m, 1 H), 7.72 - 7.84 (m, 1 H), 8.04 (ddd, J=9.3, 2.5, 1.4 Hz, 1 H), 8.07 - 8.12 (m, 1 H), 9.45 (s, 3 H) ppm; MS (DCI/NH3) m/z= 243 (M+H)+, 260 (M+NH4)+
Example 88 3-(pyrimidin-5-yl)-5-(3,4,5-triluorophenyl)-1,2,4-oxadiazole [00553] The titled compound was prepared according to the procedure of Method D using the product of Example 79C and 3,4,5-trifluorobenzoyl chloride (Aldrich). 1H
NMR (300 MHz, DMSO-d6) 6 8.24 (dd, J=8.0, 6.6 Hz, 2 H), 9.44 (s, 2 H), 9.46 (s, 1 H) ppm; MS
(DCI/NH3) m/z= 279 (M+H)+, 296 (M+NH4)+

Example 89 5-(2-Chloropyridin-4-yl)-3-(pyrimidin-5-yl)-1,2,4-oxadiazole [00554] The titled compound was prepared according to the procedure of Method D using the product of Example 79C and 2-chloroisonicotinoyl chloride (Maybridge). 1H
NMR (300 MHz, DMSO-d6) 6 8.17 (dd, J=5.1, 1.4 Hz, 1 H), 8.25 (dd, J=1.4, 0.7 Hz, 1 H), 8.78 (dd, J=5.1, 0.7 Hz, 1 H), 9.46 (s, 1 H), 9.47 (s, 2 H) ppm; MS (DCI/NH3) m/z= 260 (M+H)+, 262 (M+H)+.

Example 90 3-(Pyridin-3-yl)-5-(pyrimidin-5-yl)-1,2,4-oxadiazole [00555] The titled compound was prepared according to the procedure of Method C using N'-hydroxynicotinimidamide (Tyger) and pyrimidine-5-carboxylic acid (Maybridge). 1H
NMR (300 MHz, DMSO-d6) 6 7.68 (ddd, J=8.0, 4.9, 1.0 Hz, 1 H), 8.48 (dt, J=7.7, 2.1 Hz, 1 H), 8.84 (dd, J=4.7, 1.7 Hz, 1 H), 9.29 (dd, J=2.4, 1.0 Hz, 1 H), 9.51 (s, 1 H), 9.56 (s, 2 H) ppm; MS (DCI/NH3) m/z= 226 (M+H)+, 243 (M+NH4)+

Example 91 5-(Pyridazin-4-yl)-3-(pyridin-3-yl)-1,2,4-oxadiazole [00556] The titled compound was prepared according to the procedure of Method C using N'-hydroxynicotinimidamide (Aldrich) and pyridazine-4-carboxylic acid (Aldrich). 1H NMR
(300 MHz, CD3OD) 6 7.67 (ddd, J=8.1, 5.0, 0.8 Hz, 1 H), 8.45 (dd, J=5.4, 2.4 Hz, 1 H), 8.60 (dt, J=8.1, 1.9 Hz, 1 H), 8.78 (dd, J=5.1, 1.7 Hz, 1 H), 9.34 (dd, J=2.2, 0.8 Hz, 1 H), 9.55 (dd, J=5.4,1.4 Hz, 1 H), 9.94 (dd, J=2.2, 1.2 Hz, 1 H) ppm; MS (DCI/NH3) m/z= 226 (M+H)+.
Example 92 3-(3-(Pyridazin-4-yl)-1,2,4-oxadiazol-5-yl)benzonitrile [00557] The titled compound was prepared according to the procedure of Method D using the product of Example 83D and 3-cyanobenzoyl chloride (Aldrich). 1H NMR (300 MHz, CD3OD) 6 7.86 (t, J=7.9 Hz, 1 H), 8.09 (ddd, J=8.1, 1.4, 1.2 Hz, 1 H), 8.40 (dd, J=5.4, 2.2 Hz, 1 H), 8.55 (dt, J=7.9, 1.6 Hz, 1 H), 8.64 (t, J=2.0 Hz, 1 H), 9.46 (dd, J=5.4, 1.4 Hz, 1 H), 9.88 (dd, J=2.2, 1.4 Hz, 1 H) ppm; MS (DCI/NH3) m/z= 250 (M+H)+.
Example 93 5-(3-Fluorophenyl)-3-(pyridazin-4-yl)-1,2,4-oxadiazoleThe titled compound was prepared according to the procedure of Method D using the product of Example 83D and 3-fluorobenzoyl chloride (Aldrich). 1H NMR (300 MHz, CD3OD) 6 7.45 - 7.53 (m, 1 H), 7.69 (td, J=8.1, 5.6 Hz, 1 H), 8.00 (ddd, J=9.1, 2.6, 1.4 Hz, 1 H), 8.11 (dt, J=7.9, 1.2 Hz, 1 H), 8.39 (dd, J=5.4, 2.2 Hz, 1 H), 9.45 (dd, J=5.2, 1.2 Hz, 1 H), 9.87 (dd, J=2.2, 1.4 Hz, 1 H) ppm; MS (DCI/NH3) m/z= 243 (M+H)+.

Example 94 3-(Pyridazin-4-yl)-5-(3,4,5-trifluorobhenyl)-1,2,4-oxadiazole [00559] The titled compound was prepared according to the procedure of Method D using the product of Example 83D and 3,4,5-trifluorobenzoyl chloride (Aldrich). 1H
NMR (300 MHz, CD3OD) 6 8.04 - 8.16 (m, 2 H), 8.38 (dd, J=5.4, 2.2 Hz, 1 H), 9.46 (dd, J=5.4,1.4 Hz, 1 H), 9.86 (dd, J=2.4, 1.2 Hz, 1 H) ppm; MS (DCI/NH3) m/z= 279 (M+H)+.
Example 95 5-(3,5-difluorophenyl)-3-(pyridazin-4-yl)-1,2,4-oxadiazole [00560] The titled compound was prepared according to the procedure of Method D using the product of Example 83D and 3,5-difluorobenzoyl chloride (Aldrich). 'H NMR
(300 MHz, CD3OD) 6 7.40 (tt, J=8.9, 2.4 Hz, 1 H), 7.85 - 7.94 (m, 2 H), 8.39 (dd, J=5.4, 2.2 Hz, 1 H), 9.46 (dd, J=5.4, 1.4 Hz, 1 H), 9.87 (dd, J=2.2, 1.4 Hz, 1 H) ppm; MS
(DCI/NH3) m/z=
261 (M+H)+.

Example 96 5-(4-fluorobhenyl)-3-(pyridazin-4-yl)-1,2,4-oxadiazole [00561] The titled compound was prepared according to the procedure of Method D using the product of Example 83D and 4-fluorobenzoyl chloride (Aldrich). 1H NMR (300 MHz, CD3OD) 6 7.36 - 7.45 (m, 2 H), 8.29 - 8.40 (m, 3 H), 9.45 (dd, J=5.6, 1.2 Hz, 1 H), 9.86 (dd, J=2.4, 1.2 Hz, 1 H) ppm; MS (DCI/NH3) m/z= 243 (M+H)+.

Example 97 3-(pyridazin-4-yl)-5-(pyrimidin-5-yl)-1,2,4-oxadiazole [00562] The titled compound was prepared according to the procedure of Method C using the product of Example 83D and pyrimidine-5-carboxylic acid (Maybridge). 1H
NMR (300 MHz, CD3OD) 6 8.41 (dd, J=5.4, 2.4 Hz, 1 H), 9.44 (s, 1 H), 9.47 (dd, J=5.4, 1.4 Hz, 1 H), 9.59 (s, 2 H), 9.90 (dd, J=2.2, 1.2 Hz, 1 H) ppm; MS (DCI/NH3) m/z= 227 (M+H)+.

Example 98 3-(pyridazin-4-yl)-5-(2,3,6-trifluorobhenyl)-1,2,4-oxadiazole [00563] The titled compound was prepared according to the procedure of Method D using the product of Example 83D and 2,3,6-trifluorobenzoyl chloride (Aldrich). 1H
NMR (300 MHz, CD3OD) 6 7.28 - 7.37 (m, J=9.5, 9.5, 3.7, 2.4 Hz, 1 H), 7.67 - 7.79 (m, J=9.6, 9.6, 8.7, 4.9 Hz, 1 H), 8.39 (dd, J=5.3, 2.2 Hz, 1 H), 9.46 (dd, J=5.3, 1.2 Hz, 1 H), 9.88 (dd, J=2.2, 1.2 Hz, 1 H) ppm; MS (DCI/NH3) m/z= 279 (M+H)+.

Example 99 3-(pyridazin-4-yl)-5-(2,3,4-trifluorobhenyl)-1,2,4-oxadiazole [00564] The titled compound was prepared according to the procedure of Method D using the product of Example 83D and 2,3,4-trifluorobenzoyl chloride (Aldrich). 1H
NMR (300 MHz, CD3OD) 6 7.40 - 7.50 (m, J=9.3, 9.3, 7.0, 2.2 Hz, 1 H), 8.10 - 8.20 (m, 1 H), 8.39 (dd, J=5.4, 2.4 Hz, 1 H), 9.46 (dd, J=5.3, 1.2 Hz, 1 H), 9.87 (dd, J=2.2, 1.2 Hz, 1 H) ppm; MS
(DCI/NH3) m/z= 279 (M+H)+.
Example 100 N,N-dimethyl-N'-(4-(3-(pyridazin-4-yl)-1,2,4-oxadiazol-5-yl)phenylsulfonyl)formimidamide [00565] The titled compound was prepared according to the procedure of Method D using the product of Example 83D and 4-sulfamidobenzoyl chloride dimethylformamide complex (Alfa Aesar). 1H NMR (300 MHz, CD3OD) 6 2.95 (s, 3H), 3.18 (s, 3 H), 8.06 (d, J=8.5 Hz, 2 H), 8.29 (s, 1 H), 8.31 (dd, J=5.3, 2.2 Hz, 1 H), 8.37 (d, J=8.8 Hz, 2 H), 9.54 (dd, J=5.1, 1.4 Hz, 1 H), 9.83 (dd, J=2.4, 1.4 Hz, 1 H) ppm; MS (DCI/NH3) m/z= 359 (M+H)+.

Example 101 5-(3,4-difluorophenyl)-3-(pyrimidin-5-yl)-1,2,4-oxadiazole tosylate Example lOlA
5-(3,4-Difluorophenyl)-3-(pyrimidin-5-yl)-1,2,4-oxadiazole [00566] The titled compound was prepared according to the procedure of Method D using the product of Example 79C and 3,4-difluorobenzoyl chloride (Aldrich). 1H NMR
(300 MHz, CDC13) 6 7.35 - 7.48 (m, 1 H), 7.97 - 8.13 (m, 2 H), 9.40 (s, 1 H), 9.49 (s, 2 H) ppm;
MS (DCI/NH3) m/z 261 (M+H)+.

Example 1 O l B
5-(3,4-Difluorophenyl)-3-(pyrimidin-5-yl)-1,2,4-oxadiazole tosylate [00567] A solution of the product of Example l OlA (52 mg, 0.2 mmol) in ethyl acetate (5 mL) was stirred with a solution ofp-toluenesulfonic acid monohydrate (Aldrich, 46.0 mg, 0.24 mmol) in ethyl acetate (1.0 mL) at ambient temperature for 10 hours. The precipitates were collected by filtration and dried to give the title compound. 1H NMR (300 MHz, CDC13) 6 2.40 (s, 3 H), 7.25 (d, J= 8.2 Hz, 2 H), 7.36 - 7.53 (m, 1 H), 7.83 (d, J= 8.3 Hz, 2 H), 8.03-8.12 (m, 2 H), 9.57 (s, 1 H), 9.71 (s, 2 H); MS (DCI/NH3) m/z 261 (M+H)+.

Example 102 3-(3,4-difluorophenyl)-5-(pyrimidin-5-yl)-1,2,4-oxadiazole [00568] The titled compound was prepared according to the procedure of Method D using 3,4-difluoro-N'-hydroxybenzimidamide (Tyger) and pyrimidine-5-carbonyl chloride. The pyrimidine-5-carbonyl chloride was prepared by the reaction of pyrimidine-5-carboxylic acid (Maybridge, 138 mg, 1.0 mmol) with oxalyl chloride (Aldrich, 2 M, in CH2C12, 1.0 mL, 2.0 mmol) and a drop of dimethylformamide at room temperature over 1 hour with subsequent removal of volatiles under reduced pressure. 1H NMR (300 MHz, DMSO-d6) 6 7.72 (dt, J=10.5, 8.5 Hz, 1 H), 7.94 - 8.05 (m, 1 H), 8.11 (ddd, J=10.9, 7.7, 2.0 Hz, 1 H), 8.12 (none, 1 H), 9.51 (s, 1 H), 9.54 (s, 2 H) ppm; MS (DCI/NH3) m/z 261 (M+H)+.

Example 103 3-(Pyrimidin-5-yl)-5-(2,3,4-trifluorobhenyl)-1,2,4-oxadiazole [00569] The titled compound was prepared according to the procedure of Method D using the product of Example 79C and 2,3,4-trifluorobenzoyl chloride (Aldrich). 1H
NMR (300 MHz, DMSO-d6) 6 7.58 - 7.75 (m, 1 H), 8.03 - 8.26 (m, 1 H), 9.44 (s, 2 H), 9.45 (s, 1 H) ppm; MS (DCI/NH3) m/z 279 (M+H)+, 296 (M+NH4)+

Example 104 3-(pyrimidin-5-yl)-5-(2,3,6-trifluorophenyl)-1,2,4-oxadiazole [00570] The titled compound was prepared according to the procedure of Method D using the product of Example 79C and 2,3,6-trifluorobenzoyl chloride (Aldrich). 1H
NMR (300 MHz, DMSO-d6) 6 7.48 - 7.61 (m, 1 H), 7.89 - 8.07 (m, 1 H), 9.44 (s, 2 H), 9.45 (s, 1 H) ppm; MS (DCI/NH3) m/z 279 (M+H)+, 296 (M+NH4)+
Example 105 3-(pyrimidin-5-yl)-5-(2,3,4,5-tetrafluorophenyl)-1,2,4-oxadiazole [00571] The titled compound was prepared according to the procedure of Method D using the product of Example 79C and 2,3,4,5-tetrafluorobenzoyl chloride (Aldrich).

(300 MHz, DMSO-d6) 6 8.18 - 8.42 (m, 1 H), 9.45 (s, 2 H), 9.46 (s, 1 H) ppm;
MS
(DCI/NH3) m/z 297 (M+H)+, 314 (M+NH4)+

Example 106 5-(imidazo [ 1,5-a]pyridin-6-yl)-3-(pyridin-3-yl)-1,2,4-oxadiazole [00572] The title compound was prepared according to Method C using imidazo[1,2-a]pyridine-6-carboxylic acid (Maybridge) and N'-hydroxynicotinimidamide (Tyger). 1H
NMR (300 MHz, DMSO-d6) 6 7.67 (ddd, J=8.1, 4.8, 1.0 Hz, 1 H), 7.76 (d, J=1.4 Hz, 1 H), 7.78 - 7.92 (m, 2 H), 8.21 (s, 1 H), 8.46 (dt, J=8.2, 1.8 Hz, 1 H), 8.83 (dd, J=4.7, 1.7 Hz, 1 H), 9.27 (dd, J=2.2, 0.8 Hz, 1 H), 9.69 (dd, J=1.7, 1.0 Hz, 1 H) ppm; MS
(DCI/NH3) m/z 264(M+H)+.
Example 107 5-(1H-indol-6-yl)-3-(pyridin-3-yl)-1,2,4-oxadiazole [00573] The title compound was prepared according to Method C using 1H-indole-carboxylic acid (Aldrich) and N'-hydroxynicotinimidamide (Tyger). 1H NMR (300 MHz, DMSO-d6) 6 6.62 (ddd, J=3.0, 1.9, 0.8 Hz, 1 H), 7.62 - 7.71 (m, 2 H), 7.76 -7.89 (m, 2 H), 8.27 - 8.33 (m, J=1.4 Hz, 1 H), 8.46 (dt, J=8.2, 1.9, 1.7 Hz, 1 H), 8.82 (dd, J=4.7, 1.7 Hz, 1 H), 9.27 (dd, J=2.2, 0.8 Hz, 1 H), 11.66 (s, 1 H) ppm; MS (DCI/NH3) m/z 263 (M+H)+.

Example 108 5-(2,7-dimethyllpyrazolo [ 1,5-alpyrimidin-6-yl)-3-(pyridin-3-yl)-1,2,4-oxadiazole [00574] The title compound was prepared according Method C using 2,7-dimethylpyrazolo [ 1,5 -a]pyrimidine-6-carboxylic acid (Maybridge) and N'-hydroxynicotinimidamide (Tyger). 1H NMR (300 MHz, DMSO-d6) b2.52 (s, 3 H), 3.27 (s, 3 H), 6.77 (s, 1 H), 7.67 (ddd, J=8.0, 4.9, 0.7 Hz, 1 H), 8.48 (dt, J=8.6, 1.9 Hz, 1 H), 8.84 (dd, J=4.7, 1.7 Hz, 1 H), 9.06 (s, 1 H), 9.30 (dd, J=2.2, 0.8 Hz, 1 H), 9.30 (d, J=1.4 Hz, 1 H) ppm;
MS (DCI/NH3) m/z 293 (M+H)+.

Example 109 5-(2,2-difluorobenzo [d][ 1,3]dioxol-5-yl)-3-(pyridin-3-yl)-1,2,4-oxadiazole [00575] The title compound was prepared according to Method C using 2,2-difluorobenzo [d] [ 1,3 ] dioxole-5 -carboxylic acid (Aldrich) and N'-hydroxynicotinimidamide (Tyger). 1H NMR (300 MHz, DMSO-d6) 6 7.66 (ddd, J=8.1, 4.8, 1.0 Hz, 1 H), 7.73 (d, J=8.5 Hz, 1 H), 8.14 (dd, J=8.5, 1.7 Hz, 1 H), 8.25 (d, J=1.7 Hz, 1 H), 8.44 (dt, J=8.2, 1.9, 1.7 Hz, 1 H), 8.82 (dd, J=4.7, 1.7 Hz, 1 H), 9.25 (dd, J=2.2, 0.8 Hz, 1 H) ppm; MS
(DCI/NH3) m/z 304 (M+H)+.
Example 110 5-(2-methylbenzofuran-5 -yl)-3-(pyridin-3-yl)-1,2,4-oxadiazole [00576] The title compound was prepared according to Method C using 2-methylbenzofuran-5-carboxylic acid (Chembridge) and N'-hydroxynicotinimidamide (Tyger).
iH NMR (300 MHz, DMSO-d6) 6 3.33 (s, 3 H), 6.58 - 6.97 (m, 1 H), 7.66 (ddd, J=8.0,4.9, 1.0 Hz, 1 H), 7.78 (d, J=8.5 Hz, 1 H), 8.40 - 8.55 (m, 2 H), 8.82 (dd, J=4.7,1.7 Hz, 1 H), 9.27 (dd, J=2.2, 0.8 Hz, 1 H) ppm; MS (DCI/NH3) m/z 278 (M+H)+.

Example 111 5-(benzo [d] [ 1,2,3 ]thiadiazol-5-yl)-3-(pyridin-3-yl)-1,2,4-oxadiazole [00577] The title compound was prepared according to Method C using benzo [d] [ 1,2,3 ]thiadiazole-5 -carboxylic acid (Maybridge) and N'-hydroxynicotinimidamide (Tyger). 1H NMR (300 MHz, DMSO-d6) 6 7.69 (ddd, J=8.0, 4.9, 0.7 Hz, 1 H), 8.51 (dt, J=8.2, 1.9, 1.7 Hz, 1 H), 8.57 (dd, J=8.5, 1.7 Hz, 1 H), 8.73 (d, J=7.8 Hz, 1 H), 8.85 (dd, J=4.9, 1.5 Hz, 1 H), 9.32 (dd, J=2.4, 1.0 Hz, 1 H), 9.51 (dd, J=1.7, 0.7 Hz, 1 H) ppm; MS
(DCI/NH3) m/z 282 (M+H)+.
Example 112 5-(1H-benzo [dlimidazol-5-yl)-3-(pyridin-3-yl)-1,2,4-oxadiazole [00578] The title compound was prepared according to Method C using 1H-benzo[d]imidazole-5-carboxylic acid (Aldrich) and N'-hydroxynicotinimidamide (Tyger). 1H
NMR (300 MHz, DMSO-d6) 6 7.66 (ddd, J=8.1, 4.8, 1.0 Hz, 1 H), 7.75 - 7.98 (m, 1 H), 7.99 - 8.17 (m, 1 H), 8.34 - 8.56 (m, 3 H), 8.82 (dd, J=5.1, 1.7 Hz, 1 H), 9.28 (dd, J=2.2, 0.8 Hz, 1 H) ppm; MS (DCI/NH3) m/z 264 (M+H)+.

Example 113 5-(1H-benzo [d][ 1,2,3]triazol-5-yl)-3-(pyridin-3-yl)-1,2,4-oxadiazole [00579] The title compound was prepared according to Method C using 1H-benzo[d][1,2,3]triazole-5-carboxylic acid (Aldrich) andN'-hydroxynicotinimidamide (Tyger).
iH NMR (300 MHz, DMSO-d6) 6 7.67 (ddd, J=8.0, 4.9, 0.7 Hz, 1 H), 8.10 - 8.20 (m, 1 H), 8.22 - 8.31 (m, 1 H), 8.49 (dt, J=8.1, 2.0, 1.7 Hz, 1 H), 8.75 - 8.92 (m, 2 H), 9.30 (dd, J=2.2, 0.8 Hz, 1 H) ppm; MS (DCI/NH3) m/z 265 (M+H)+.

Example 114 5-(benzo [dlthiazol-5-yl)-3-(pyridin-3-yl)-1,2,4-oxadiazole [00580] The title compound was prepared according to Method C using benzo[d]thiazole-5-carboxylic acid (Apollo) and N'-hydroxynicotinimidamide (Tyger). 1H NMR (300 MHz, DMSO-d6) 6 7.64 - 7.73 (m, 1 H), 8.35 (s, 1 H), 8.36 (s, 1 H), 8.48 (dt, J=8.1, 2.0, 1.7 Hz, 1 H), 8.83 (dd, J=4.7, 1.7 Hz, 1 H), 9.17 (t, J=1.2 Hz, 1 H), 9.29 (dd, J=2.2, 0.8 Hz, 1 H), 9.66 (s, 1 H) ppm; MS (DCI/NH3) m/z 281 (M+H)+.

Example 115 3-(pyridin-3-yl)-5-(1H-pyrrolo[2,3-blpyridin-5-XI)-1,2,4-oxadiazole [00581] The title compound was prepared according to Method C using 1H-pyrrolo[2,3-b]pyridine-5-carboxylic acid (Adesis) and N'-hydroxynicotinimidamide (Tyger).

(300 MHz, DMSO-d6) 6 6.71 (d, J=3.4 Hz, 1 H), 7.66 (ddd, J=7.9, 4.8, 0.8 Hz, 1 H), 7.70 (d, J=3.4 Hz, 1 H), 8.47 (dt, J=8.1, 1.9 Hz, 1 H), 8.78 - 8.86 (m, 2 H), 9.05 (d, J=2.0 Hz, 1 H), 9.28 (dd, J=2.2, 0.8 Hz, 1 H) ppm; MS (DCI/NH3) m/z 264 (M+H)+.
Example 116 5-(1H-indol-5-yl)-3-(pyridin-3-yl)-1,2,4-oxadiazole [00582] The title compound was prepared according to Method C using 1H-indole-carboxylic acid (Aldrich) and N'-hydroxynicotinimidamide (Tyger). 1H NMR (300 MHz, DMSO-d6) 6 6.69 (dt, J=2.3, 1.4 Hz, 1 H), 7.55 (t, J=2.7 Hz, 1 H), 7.62 - 7.70 (m, 2 H), 7.94 (dd, J=8.8, 1.7 Hz, 1 H), 8.46 (dt, J=8.1, 1.9 Hz, 1 H), 8.49 - 8.52 (m, 1 H), 8.81 (dd, J=4.7, 1.7 Hz, 1 H), 9.27 (dd, J=2.2, 0.8 Hz, 1 H) ppm; MS (DCI/NH3) m/z 263 (M+H)+.

Example 117 5-(benzofuran-5-yl)-3-(pyridin-3-yl)-1,2,4-oxadiazole [00583] The title compound was prepared according to Method C using benzofuran-carboxylic acid (Aldrich) and N'-hydroxynicotinimidamide (Tyger). 1H NMR (300 MHz, DMSO-d6) 6 7.20 (dd, J=2.0, 1.0 Hz, 1 H), 7.66 (ddd, J=7.9, 4.8, 0.8 Hz, 1 H), 7.91 (dt, J=8.6, 1.0, 0.8 Hz, 1 H), 8.14 - 8.24 (m, 2 H), 8.47 (dt, J=7.9, 2.0 Hz, 1 H), 8.60 (d, J=1.4 Hz, 1 H), 8.82 (dd, J=4.7, 1.7 Hz, 1 H), 9.28 (dd, J=2.2, 0.8 Hz, 1 H) ppm; MS
(DCI/NH3) m/z 264 (M+H)+.
Example 118 5-(1-methyl-1 H-benzo [dlimidazol-5-yl)-3-(pyridin-3-yl)-1,2,4-oxadiazole [00584] The title compound was prepared according to Method C using 1-methyl-lH-benzo[d]imidazole-5-carboxylic acid (Maybridge) and N'-hydroxynicotinimidamide (Tyger).
iH NMR (300 MHz, DMSO-d6) b7.65 (ddd, J=8.0, 4.9, 0.7 Hz, 1 H), 7.68 - 7.88 (m, J=8.8 Hz, 1 H), 7.87 - 8.10 (m, 2 H), 8.14 - 8.37 (m, 1 H), 8.46 (dt, J=8.1, 1.9 Hz, 1 H), 8.82 (dd, J=4.7, 1.7 Hz, 1 H), 9.27 (dd, J=2.0, 0.7 Hz, 1 H) ppm; MS (DCI/NH3) m/z 278 (M+H)+.

Example 119 3-(imidazo [ 1,2-a]pyridin-6-yl)-5-(pyridin-3-yl)-1,2,4-oxadiazole [00585] The title compound was prepared according to Method D using N'-hydroxyimidazo[1,2-a]pyridine-6-carboximidamide (Bionet) and nicotinoyl chloride, hydrochloric Acid (Aldrich). 1H NMR (300 MHz, DMSO-d6) 6 7.62 - 7.91 (m, 4 H), 8.20 (s, 1 H), 8.56 (dt, J=8.1, 1.9 Hz, 1 H), 8.92 (dd, J=4.7, 1.7 Hz, 1 H) ppm; MS
(DCI/NH3) m/z 264 (M+H)+.
Example 120 5-(6-chloropyridin-3-yl)-3-(imidazo [ l ,2-alpyridin-6-yl)-1,2,4-oxadiazole [00586] The title compound was prepared according to Method D using N'-hydroxyimidazo[1,2-a]pyridine-6-carboximidamide (Bionet) and 6-chloronicotinoyl chloride (Aldrich). 1H NMR (300 MHz, DMSO-d6) 6 7.70 (d, J=1.2 Hz, 1 H), 7.74 - 7.84 (m, 2 H), 7.87 (d, J=8.3 Hz, 1 H), 8.20 (s, 1 H), 8.59 (dd, J=8.5, 2.6 Hz, 1 H), 9.20 (dd, J=2.4, 0.8 Hz, 1 H), 9.47 (t, J=1.4 Hz, 1 H) ppm; MS (DCI/NH3) m/z 298 (M+H)+, 300 (M+H)+.

Example 121 5-(6-fluroyridin-3-yl)-3-(imidazo [ l ,2-a]pyridin-6-yl)-1,2,4-oxadiazole [00587] The title compound was prepared according to Method C using N'-hydroxyimidazo[1,2-a]pyridine-6-carboximidamide (Bionet) and 6-fluoronicotinic acid (Frontier). 1H NMR (300 MHz, DMSO-d6) 6 7.55 (ddd, J=8.5, 2.7, 0.7 Hz, 1 H), 7.70 (d, J=1.4 Hz, 1 H), 7.73 - 7.86 (m, 2 H), 8.20 (s, 1 H), 8.75 (ddd, J=8.1, 2.5 Hz, 1 H), 9.08 (dt, J=1.7, 0.8 Hz, 1 H), 9.46 (dd, J=1.7, 1.0 Hz, 1 H) ppm; MS (DCI/NH3) m/z 282 (M+H)+.
Example 122 5-(5-fluroyridin-3-yl)-3-(imidazo [ l ,2-a]pyridin-6-yl)-1,2,4-oxadiazole [00588] The title compound was prepared according to method C using N'-hydroxyimidazo[1,2-a]pyridine-6-carboximidamide (Bionet) and 5-fluoronicotinic acid (Frontier). 1H NMR (300 MHz, DMSO-d6) 6 7.71 (d, J=1.4 Hz, 1 H), 7.74 - 7.88 (m, 2 H), 8.20 (s, 1 H), 8.51 (ddd, J=9.0, 2.9, 1.7 Hz, 1 H), 8.97 (d, J=3.1 Hz, 1 H), 9.24 (t, J=1.5 Hz, 1 H), 9.47 (t, J=1.4 Hz, 1 H) ppm; MS (DCI/NH3) m/z 282 (M+H)+.

Example 123 5-(1H-indazol-5-yl)-3-(pyridin-3-yl)-1,2,4-oxadiazole [00589] The title compound was prepared according to Method C using 1H-indazole-5-carboxylic acid (ABCR) and N'-hydroxynicotinimidamide (Tyger). 'H NMR (300 MHz, DMSO-d6) 6 7.7 (dd, J=7.9, 4.8 Hz, 1 H), 7.8 (d, J=8.7 Hz, 1 H), 8.2 (dd, J=8.7, 1.6 Hz, 1 H), 8.4 (s, 1 H) 8.5 (dt, J=7.9, 1.8 Hz, 1 H), 8.7 (s, 1 H) 8.8 (dd, J=4.8, 1.6 Hz, 1 H), 9.3 (d, J=2.0 Hz, 1 H), 13.6 (s, 1 H) ppm; MS (DCI/NH3) m/z 264 (M+H)+.

Example 124 5-([ 1,2,4]triazolo [4,3-a]pyridin-6-yl)-3-(pyridin-3-yl)-1,2,4-oxadiazole Example 124A
5-(6-Chloropyridin-3-yl)-3-(pyridin-3-yl)-1,2,4-oxadiazole [00590] The title compound was prepared according to Method D using N'-hydroxynicotinimidamide (Tyger) and 6-chloronicotinoyl chloride (Aldrich). 'H
NMR (300 MHz, CDC13) 6 7.48 (dd, J=7.6, 5.3 Hz, 1 H), 7.57 (d, J=8.5 Hz, 1 H), 8.40 -8.48 (m, 2 H), 8.80 (dd, J=4.7,1.7 Hz, 1 H), 9.24 (d, J=2.4 Hz, 1 H), 9.40 (d, J=2.4 Hz, 1 H) ppm; MS
(DCI/NH3) m/z 259 (M+H)+, 261 (M+H)+.

Example 124B
5-(6-H, d~yllpyridin-3-yl)-3-(pyridin-3-yl)-1,2,4-oxadiazole [00591] A suspension of the product of Example 126A (260 mg, 1 mmol) in EtOH
(15 mL) was stirred with hydrazine (Aldrich, 50 mg, 2.5 mmol) at refluxing temperature for 2 h and then cooled to room temperature. The precipitates were collected by filtration, washed with addition cooled EtOH (5 mL) and dried to gave the title compound. 'NMR (DMSO-d6): 6 6.77 - 7.08 (m, 1 H), 7.35 - 7.44 (m, 2 H), 7.63 (ddd, J=8.0, 4.9, 1.0 Hz, 1 H), 7.79 (tt, J=7.6, 1.9 Hz, 1 H), 8.12 (dd, J=8.8, 2.4 Hz, 1 H), 8.41 (dt, J=8.1, 1.9 Hz, 1 H), 8.58 (dt, J=4.0, 1.9 Hz, 1 H), 8.75 - 8.84 (m, 1 H), 9.22 (dd, J=2.2, 0.8 Hz, 1 H) ppm, MS
(DCI/NH3) m/z= 255 (M+H)+.

Example 124C
5-([1,2,4]triazolo[4,3-alyridin-6-yl)-3-(pyridin-3-yl)-1,2,4-oxadiazole [00592] A suspension of the product of Example 126B (80 mg, 0.31 mmol) in triethoxymethane (Aldrich, 4 mL) was heated to reflux for 16 hours. The reaction mixture was cooled to room temperature and the volatiles were removed under vacuum.
The residue was purified with chromatography (Si02, EtOAc/CH2C12 = 1/9) to give the title compound.
'HNMR (DMSO-d6): 6 7.68 (dd, J=8.1, 5.0 Hz, 1 H), 7.90 - 8.11 (m, 2 H), 8.46 (dt, J=7.9, 2.0 Hz, 1 H), 8.84 (dd, J=4.8, 1.6 Hz, 1 H), 9.27 (d, J=2.0 Hz, 1 H), 9.44 (s, 1 H), 9.68 (s, 1 H) ppm; MS (DCI/NH3) m/z= 265 (M+H)+.

Example 125 5-(3-(pyridin-3-yl)-1,2,4-oxadiazol-5-yl)benzo [dloxazol-2(3H)-one [00593] The title compound was prepared according to Method C using 2-oxo-2,3-dihydrobenzo[d]oxazole-5-carboxylic acid (Matrix) and N'-hydroxynicotinimidamide (Tyger). 1H NMR (300 MHz, CD3OD) 6 7.40 (d, J=8.5 Hz, 1 H), 7.64 (ddd, J=8.0, 5.0, 0.9 Hz, 1 H), 7.90 (d, J=1.7 Hz, 1 H), 8.01 (dd, J=8.3, 1.9 Hz, 1 H), 8.55 (dt, J=7.8, 1.9 Hz, 1 H), 8.74 (dd, J=4.8, 1.7 Hz, 1 H), 9.29 (dd, J=2.2, 0.9 Hz, 1 H). ppm; MS
(DCI/NH3) m/z 281 (M+H)+.

Example 126 5-(3-(pyridin-3-yl)-1,2,4-oxadiazol-5-yl)-1 H-benzo [dlimidazole-2(3H)-thione [00594] The title compound was prepared according to Method C using 2-mercapto-benzimidazolecarboxylic acid (Princeton) and N'-hydroxynicotinimidamide (Tyger). 1H

NMR (300 MHz, CD3OD) 6 7.38 (d, J=8.5 Hz, 1 H), 7.65 (ddd, J=8.0, 4.9, 0.7 Hz, 1 H), 7.86 (d, J=1.0 Hz, 1 H), 8.00 (dd, J=8.1, 1.7 Hz, 1 H), 8.45 (dt, J=8.0, 1.9 Hz, 1 H), 8.81 (dd, J=4.9, 1.5 Hz, 1 H), 9.26 (dd, J=2.2, 0.9 Hz, 1 H) ppm;. MS (DCI/NH3) m/z 296 (M+H)+.
Example 127 1,3-dimethyl-5-(3-(pyridin-3-yl)-1,2,4-oxadiazol-5-yl)-1 H-benzo [dlimidazol-2(3H)-one [00595] The title compound was prepared according to Method C using 1,3-dimethyl-2-oxo-2,3-dihydro-lH-benzoimidazole-5-carboxylic acid (Matrix) andN'-hydroxynicotinimidamide (Tyger). 1H NMR (300 MHz, CD3OD) 6 3.49 (s, 3 H), 3.53 (s, 3 H), 7.36 (d, J=8.3 Hz, 1 H), 7.64 (ddd, J=8.0, 5.1, 0.8 Hz, 1 H), 7.98 (d, J=1.6 Hz, 1 H), 8.08 (dd, J=8.3, 1.6 Hz, 1 H), 8.55 (dt, J=8.0, 1.9 Hz, 1 H), 8.73 (dd, J=4.8, 1.6 Hz, 1 H) 9.29 (d, J=1.2 Hz, 1 H). MS (DCI/NH3) m/z 308 (M+H)+.

Example 128 6-(3-(pyridin-3-yl)-1,2,4-oxadiazol-5-yl)benzo[dloxazol-2(3H)-one [00596] The title compound was prepared according to Method C using 2-oxo-2,3-dihydrobenzo[d]oxazole-6-carboxylic acid (Eur. J. Med. Chem. 1974, 9, 491-6.) and N'-hydroxynicotinimidamide (Tyger). 1H NMR (300 MHz, DMSO-d6) 6 7.35 (d, J=8.7 Hz, 1 H), 7.65 (dd, J=7.5, 4.4 Hz, 1 H), 8.04 (d, J=1.6 Hz, 1 H), 8.07 (s, 1 H), 8.44 (dt, J=7.9, 2.0 Hz, 1 H), 8.82 (dd, J=4.8, 1.6 Hz, 1 H), 9.25 (d, J=1.6 Hz, 1 H) ppm; MS (DCI/NH3) m/z 281 (M+H)+.

Example 129 5-(3-(pyridin-3-yl)-1,2,4-oxadiazol-5-yl)-1H-benzo[dlimidazol-2(3H)-one trifluoroacetic Acid [00597] A solution of 2-oxo-2,3-dihydro-lH-benzo[d]imidazole-5-carboxylic acid (Princeton, 178 mg, 1.0 mmol) in CH2Cl2 (anhydrous, 5.0 mL) was stirred with oxalyl chloride (Aldrich, 2 M, in CH2C12, 1.0 mL) in the presence of 2 drops dimethylformamide at room temperature for 1 hour. It was then concentrated and dried under reduced pressure. The residue was dissolved in pyridine (5.00 mL) and then stirred with N'-hydroxynicotinimidamide (Tyger, 137 mg, 1.0 mmol) at 100 C for 16 hours. The reaction mixture was cooled to ambient temperature, diluted with EtOAc (50 mL), washed with water (2 x 10 mL) and brine. The organic solution was concentrated under reduced pressure, purified with preparative HPLC [Waters, column: XbridgeTM Prep C18 5 m, OBDTM
30 x 100 mm, solvent: acetonitrile/water(v. I% TFA), 5/95 to 95/5, flow rate of 40 mL/minute.
Fractions were collected based upon UV signal threshold.] the title compound.
1H NMR (300 MHz, CD3OD) 6 7.25 (d, J=8.3 Hz, 1 H), 7.75 (ddd, J=7.9, 5.2, 0.8 Hz, 1 H), 7.88 (d, J=1.6 Hz, 1 H), 7.99 (dd, J=8.3, 1.6 Hz, 1 H), 8.68 (dt, J=7.9, 1.8 Hz, 1 H), 8.79 (dd, J=5.2, 1.6 Hz, 1 H), 9.33 (d, J=1.2 Hz, 1 H) ppm. MS (DCI/NH3) m/z 280 (M+H)+.
Example 130 6-(3-(Pyridin-3-yl)-1,2,4-oxadiazol-5-yl)benzo [dloxazol-2-amine Example 130A
2-nitro-5-(3-(pyridin-3-yl)-1,2,4-oxadiazol-5-yl)phenol [00598] The title compound was prepared according to Method C using 3-hydroxy-nitrobenzoic acid (Aldrich) and N'-hydroxynicotinimidamide (Tyger). 1H NMR
(300 MHz, DMSO-d6) 6 7.67 (ddd, J=7.9, 4.8, 0.8 Hz, 1 H), 7.76 (dd, J=8.5, 1.8 Hz, 1 H), 7.92 (d, J=1.6 Hz, 1 H), 8.12 (d, J=8.3 Hz, 1 H), 8.45 (dt, J=7.9, 2.0 Hz, 1 H), 8.83 (dd, J=4.8, 1.6 Hz, 1 H), 9.26 (d, J=1.2 Hz, 1 H)) ppm; MS (DCI/NH3) m/z 285 (M+H)+.

Example 130B
2-amino-5-(3-(pyridin-3-yl)-1,2,4-oxadiazol-5-yl)phenol [00599] A solution of the product of Example 132A (1.0 g, 3.52 mmol) in THE
(20.0 mL) was stirred with Raney nickel (Aldrich, 4.0 g) under H2 (30 psi) for 2 hours.
The catalyst was then carefully removed by filtration and the reaction mixture was concentrated to give the title compound. 1H NMR (300 MHz, DMSO-d6) 6 5.67 (s, 2 H), 6.74 (d, J=8.1 Hz, 1 H), 7.40 - 7.52 (m, 2 H), 7.62 (dd, J=8.0, 4.2 Hz, 1 H), 8.39 (dt, J=8.0, 2.0, 1.9 Hz, 1 H), 8.78 (dd, J=4.7, 1.7 Hz, 1 H), 9.20 (d, J=1.7 Hz, 1 H), 9.65 - 9.98 (m, J=3.4 Hz, 1 H) ppm; MS
(DCI/NH3) m/z 255 (M+H)+.
Example 130C
6-(3-(Pyridin-3-yl)-1,2,4-oxadiazol-5-yl)benzo [dloxazol-2-amine [00600] To a solution of the product of Example 132B (254 mg, 1.0 mmol) in THE
(20 ml) was added cyanic bromide (Aldrich, 106 mg, 1.0 mmol) and triethylamine (Aldrich, 0.14 ml, 1.0 mmol). The mixture was then stirred at 50 C for 10 h, cooled to ambient temperature, triturated with water (40 mL). The precipitate was filtered and dried under vacuum to give the titled compound. 1H NMR (300 MHz, DMSO-d6) 6 7.42 (d, J=8.1 Hz, 1 H), 7.65 (dd, J=7.8, 5.1 Hz, 1 H), 7.98 (s, 2 H), 8.02 (dd, J=8.1, 1.7 Hz, 1 H), 8.12 (d, J=1.7 Hz, 1 H), 8.44 (dt, J=8.1, 1.9 Hz, 1 H), 8.81 (dd, J=4.7, 1.7 Hz, 1 H), 9.26 (d, J=1.4 Hz, 1 H) ppm; MS
(DCI/NH3) m/z 280 (M+H)+.
Example 131 6-(3-(pyridin-3-yl)-1,2,4-oxadiazol-5-yl)benzo [dloxazole [00601] To a solution of the product of Example 132B (508 mg, 2.0 mmol) in triethyl orthoformate (Aldrich, 20 mL, 120 mmol) was stirred at 100 C for 10 hours. It was then cooled to ambient temperature, and then triturated with hexanes (50 mL). The precipitate was filtered and dried under vacuum to give the titled compound. 1H NMR (300 MHz, DMSO-d6) 6 7.67 (ddd, J=7.9, 4.8, 0.8 Hz, 1 H), 8.10 (d, J=8.3 Hz, 1 H), 8.28 (dd, J=8.3, 1.6 Hz, 1 H), 8.48 (dt, J=7.9, 2.0 Hz, 1 H), 8.66 (d, J=1.6 Hz, 1 H), 8.83 (dd, J=4.8, 1.6 Hz, 1 H), 9.04 (s, 1 H), 9.29 (d, J=2.4 Hz, 1 H) ppm; MS (DCI/NH3) m/z 265 (M+H)+.

Example 132 5-(5-(pyridin-3-yl)-1,2,4-oxadiazol-3-yl)benzo [dloxazol-2(3H)-one Example 132A
2-oxo-2,3-dihydrobenzo Lloxazole-5-carbonitrile [00602] A solution of 3-Amino-4-hydroxybenzonitrile (Betapharma, 537 mg, 4 mmol) in CH2C12 (20 ml) was stirred with N,N'-carbonyldiimidazole (Aldrich, 713 mg, 4.40 mmol) at room temperature for 16 hours. It was then concentrated under reduced pressure. The residue was stirred with water (10 mL) for 5 minutes, and extracted with EtOAc (3 x 10 mL). The combined extracts were concentrated to the title compound. 1H NMR (300 MHz, CD3OD) 6 7.17 - 7.22 (m, 1 H), 7.28 - 7.34 (m, 2 H) ppm; MS (DCI/NH3) m/z 178 (M+NH4)+

Example 132B
N'-H. day-2-oxo-2,3-dihydrobenzo Lloxazole-5-carboximidamide [00603] A solution of the product from Example 134A (300 mg, 1.9 mmol), hydroxylammonium chloride (Aldrich, 64.0 mg, 1.9 mmol) and triethylamine (Aldrich, 227 mg, 2.3 mmol), in methanol (10 mL) was stirred at 65 C for 2 h, cooled to ambient temperature, then concentrated under reduced pressure. The residue was triturated with EtOAc (10 mL). The precipitate was filtered and dried under vacuum to give the titled compound. 1H NMR (300 MHz, CD3OD) 6 7.22 (d, J=8.33 Hz, 1 H) 7.34-7.43 (m, 2 H). MS
(DCI/NH3) m/z 194 (M+H)+.
Example 132C
5-(5-(pyridin-3-yl)-1,2,4-oxadiazol-3-yl)benzo[dloxazol-2(3H)-one [00604] The title compound was prepared according to Method D using the product of Example 134B and nicotinoyl chloride (Aldrich). 1H NMR (300 MHz, CD3OD) 6 7.51 (d, J=8.7 Hz, 1 H), 7.68 - 7.75 (m, 2 H), 7.87 (dd, J=8.3, 2.0 Hz, 1 H), 8.57 (dt, J=7.9, 2.0 Hz, 1 H), 8.90 (dd, J=5.0, 1.8 Hz, 1 H), 9.36 (d, J=1.6 Hz, 1 H) ppm. MS (DCI/NH3) m/z 281 (M+H)+.

Example 133 5-(5-(6-chloropyridin-3-yl)-1,2,4-oxadiazol-3-yl)benzo [dloxazol-2(3H)-one [00605] The title compound was prepared according to Method D using the product of Example 134B and 6-chloronicotinoyl chloride (Aldrich). 1H NMR (300 MHz, CD3OD) 6 7.33 (d, J=8.3 Hz, 1 H), 7.73 (dd, J=8.3, 0.8 Hz, 1 H), 7.82 (d, J=1.6 Hz, 1 H), 7.91 (dd, J=8.3, 1.6 Hz, 1 H), 8.57 (dd, J=8.3, 2.4 Hz, 1 H), 9.19 (d, J=2.4 Hz, 1 H) ppm. MS
(DCI/NH3) m/z 315 317 (M+H)+.

Example 134 5 -(benzo [d][ 1,3 ] dioxol-5-yl)-3-(pyridin-3-vi)-1,2,4-oxadiazole [00606] The title compound was prepared according to method C using benzo[d][1,3]dioxole-5-carboxylic acid (Aldrich) and N'-hydroxynicotinimidamide (Tyger).
iH NMR (300 MHz, DMSO-d6) 6 6.22 (s, 2 H), 7.20 (d, J=8.1 Hz, 1 H), 7.61 -7.69 (m, 2 H), 7.81 (dd, J=8.1, 1.7 Hz, 1 H), 8.42 (dt, J=8.2, 1.9, 1.7 Hz, 1 H), 8.81 (dd, J=4.7, 1.7 Hz, 1 H), 9.24 (dd, J=2.2, 0.8 Hz, 1 H) ppm; MS (DCI/NH3) m/z 268 (M+H)+.
[00557] In addition to the specific compounds, one of ordinary skill in the art would readily recognize that a variety of pharmaceutically acceptable salts, esters, amides, and prodrugs of a parent compound also could be incorporated into a composition, method, or article of manufacture of the present invention.
[00558] Suitable pharmaceutically acceptable basic addition salts include, but are not limited to cations based on alkali metals or alkaline earth metals such as lithium, sodium, potassium, calcium, magnesium and aluminum salts and the like and nontoxic quaternary ammonia and amine cations including ammonium, tetramethylammonium, tetraethylammonium, methylamine, dimethylamine, trimethylamine, triethylamine, diethylamine, ethylamine and the like. Other representative organic amines useful for the formation of base addition salts include ethylenediamine, ethanolamine, diethanolamine, piperidine, piperazine and the like.
[00559] Other possible compounds include pharmaceutically acceptable amides and esters.
For a description of pharmaceutically acceptable esters as prodrugs, see Bundgaard, E., ed., (1985) Design of Prodrugs, Elsevier Science Publishers, Amsterdam, which is hereby incorporated by reference. These esters are typically formed from the corresponding carboxylic acid and an alcohol. Generally, ester formation can be accomplished via conventional synthetic techniques. (See, e.g., March Advanced Organic Chemistry, 3rd Ed., John Wiley & Sons, New York p. 1157 (1985) and references cited therein, and Mark et al.
Encyclopedia of Chemical Technology, John Wiley & Sons, New York (1980), both of which are hereby incorporated by reference. The alcohol component of the ester will generally comprise (i) a C2 -C 12 aliphatic alcohol that can or can not contain one or more double bonds and can or can not contain branched carbons or (ii) a C7 -C 12 aromatic or heteroaromatic alcohols. This invention also contemplates the use of those compositions, which are both esters as described herein, and at the same time are the pharmaceutically acceptable salts thereof.
[00560] For a description of pharmaceutically acceptable amides as prodrugs, see Bundgaard, H., Ed., (1985) Design of Prodrugs, Elsevier Science Publishers, Amsterdam.
These amides are typically formed from the corresponding carboxylic acid and an amine.
Generally, amide formation can be accomplished via conventional synthetic techniques. (See, e.g., Advanced Organic Chemistry, 3rd Ed., John Wiley & Sons, New York, p.
1152 (1985) and Mark et al. Encyclopedia of Chemical Technology, John Wiley & Sons, New York (1980), both of which are hereby incorporated by reference. This invention also contemplates the use of those compositions, which are amides, as described herein, and at the same time are the pharmaceutically acceptable salts thereof.
[00561] It also will be readily apparent to one with skill in the art that the compounds can be generated in vivo by administration of a drug precursor which, following administration, releases the drug in vivo via a chemical or physiological process (e.g., a parent compound on being brought to the physiological pH or through enzyme action is converted to the desired drug form).

Administration [00562] As noted above, it has been discovered that pain can be treated by concurrently administering to a patient (e.g., a mammal, such as a human) in need thereof, an a4(32 PAM
and an a4(32 receptor ligand. Such combination may be especially useful in expanding the dosage range for obtaining therapeutically beneficial effects.

[00563] The dosage range at which the a4(32 PAM and an a4(32 receptor ligand will be administered concurrently can vary widely. The specific dosage will be chosen by the patient's physician taking into account the particular compounds chosen, the severity of the patient's illness, any other medical conditions or diseases the patient is suffering from, other drugs the patient is taking and their potential to cause an interaction or adverse event, the patient's previous response to medication, and other factors. Suitable dosage ranges for the a4(32 PAM are from about 0.0001 mg/kg to 100 mg/kg of body weight. Suitable dosage ranges for the a4(32 receptor ligand are from about 0.000 1 mg/kg to 100 mg/kg of body weight.

[00564] The a4(32 PAM and an a4(32 receptor ligand should be administered concurrently in amounts that are effective to treat the patient's pain, cognitive disorder, or related condition. In more general terms, one would create a combination of the present invention by choosing a dosage of an a4(32 PAM and an a4(32 receptor ligand according to the spirit of the guidelines presented above.

[00565] The invention also is carried out by administering an a4(32PAM
together with an a4(32receptor ligand in any manner which provides effective levels of the compounds in the body at the same time. Typically, the combination will be administered orally.
[00566] However, the invention is not limited to oral administration. The invention should be construed to cover any route of administration that is appropriate for the medications involved and for the patient. For example, transdermal administration may be very desirable for patients who are forgetful or petulant about taking oral medicine.
Injections may be appropriate for patients refusing their medication. One of the drugs may be administered by one route, such as oral, and the others may be administered by the transdermal, percutaneous, intravenous, intramuscular, intranasal, or intrarectal route, in particular circumstances. The route of administration may be varied in any way, limited by the physical properties of the drugs and the convenience of the patient and the caregiver.
[00567] Based on the diversity of the mechanisms underlying chronic pain (e.g.
nociceptive or neuropathic, degrees of pain intensity, various etiologies etc), currently available pain medications are not efficacious in all patients or in all pain conditions.
Analgesics can be broadly categorized as non-opioid analgesics (acetaminophen and non-steroidal anti-inflammatory drugs (NSAIDs)), opioid analgesics (morphine) and adjuvant analgesics or co-analgesics (antiepileptic drugs and antidepressants). In a simplified classification, non-opioid analgesics are mostly used to relieve mild to moderate nociceptive pain, adjuvant analgesics (gabapentin, pregabalin) are used to relieve neuropathic pain, and opioid analgesics are used to treat severe pain of all origins, depending on the dose prescribed.
[00568] Nicotinic acetylcholine receptor ligands act at multiple locations throughout the pain pathway to relieve pain. Nicotinic acetylcholine receptor ligands are found on primary sensory neurons (periphery) where nociceptive information is initiated, in the cell body regions of these neurons (i.e. the dorsal root ganglion or DRG), the dorsal spinal cord where the first pain synapse is located, in the brainstem cell body regions that control descending innervation, as well as in the higher brain regions that integrate and perceive sensory information such as the thalamus and the cortex. The current theory supported by evidence from multiple sources (reviewed in Decker et al., Curr Topics Med Chem, 4:
369, 2004) is that anti-nociceptive effects of nAChR ligands are mediated by activation of brain stem nuclei with descending inhibitory inputs to the spinal cord. Additional pathways may also mediate analgesic effects of nAChR agonists in persistent or neuropathic pain.
[00569] Another aspect of the invention is the potential to enhance efficacy of other medications used for treating pain when combined with an a4(32 PAM. As noted above, examples of currently used drugs include opioids, gabapentin, pregabalin, duloxetine and others. Novel mechanisms such as cannabinoids, vanilloid receptor antagonists, calcium channel blockers and sodium channel blockers are also being developed for the treatment of pain. For many of these mechanisms, it is emerging that a component of efficacy may be driven by activation of descending inhibitory inputs. For example, opioid analgesics can block pain transmission, in part by increasing descending inhibitory pathways to modulate pain transmission at the spinal level (Pastemack, G.W., Clin Neuropaharmcol.
16: 1, 1993;
Lauretti, G.T., Expert Reviews in Neurotherapeutics, 6: 613-622. 2006). Since these drugs exert their effect via activating descending inhibitory inputs, and these pathways can be shared or commonly activated by a4(32 nAChR ligands, it is anticipated that co-administration of a4(32 selective PAMs can lead to enhanced efficacy of other analgesic agents by amplifying the descending inhibitory control of spinal cord activation. Thus, combination with a4(32 PAMs enables the opportunity to create analgesic medications with either a broader or superior spectrum of efficacy that would improve the treatment of chronic pain.
[00570] Other nAChR-mediated diseases or disorders also can benefit from such concurrent administration. The combination of a4(32 nAChR ligands and a4(32 selective PAMs can be used for treatment of diseases or disorders related to the cholinergic system of the central nervous system, the peripheral nervous system, diseases or disorders related to smooth muscle contraction, endocrine diseases or disorders, diseases or disorders related to neuro-degeneration, diseases or disorders related to inflammation, and withdrawal symptoms caused by the termination of abuse of chemical substances, in for example nicotine, as well as pain. In a particular embodiment, the combination is useful for conditions and disorders related to attention deficit disorder, attention deficit hyperactivity disorder (ADHD), Alzheimer's disease (AD), schizophrenia, mild cognitive impairment, age-associated memory impairment (AAMI), senile dementia, AIDS dementia, Pick's Disease, dementia associated with Lewy bodies, dementia associated with Down's syndrome, schizophrenia, smoking cessation, substance abuse, amyotrophic lateral sclerosis, Huntington's disease, diminished CNS function associated with traumatic brain injury, acute pain, post-surgical pain, chronic pain, inflammatory pain, neuropathic pain, infertility, lack of circulation, need for new blood vessel growth associated with wound healing, more particularly circulation around a vascular occlusion, need for new blood vessel growth associated with vascularization of skin grafts, ischemia, inflammation, sepsis, wound healing, and other complications associated with diabetes, among other systemic and neuroimmunomodulatory activities. The method is useful for conditions and disorders related to conditions and disorders characterized by neuropsychological and cognitive dysfunction, for example in Alzheimer's disease, bipolar disorder, schizophrenia, schizoaffective disorder, and other related disorders characterized by neuropsychological and cognitive dysfunction, in particular.
[00571] For example, one embodiment relates to a method of use for treating or preventing a condition or disorder characterized by attention or cognitive dysfunction, such as Alzhimer's disease and ADHD, among other condition and disorders. The method comprises the step of administering a therapeutically effective amount of a nicotinic acetylcholine receptor subtype a4(32 positive allosteric modulator to a subject in need thereof in combination with a drug that improves cholinergic function. Examples of such drugs are nicotinic acetylcholine receptor ligands and acetylcholinesterase inhibitors.
[00572] Another method of use relates to treating or preventing a condition or disorder characterized by neuropsychological dysfunction, for example scizhophrenia, wherein the method comprises the step of administering a therapeutically effective amount of a nicotinic acetylcholine receptor subtype a4(32 positive allosteric modulator to a subject in need thereof in combination with an antipsychotic agent.

BIOLOGICAL ACTIVITY

[00573] Example A: a4(32 Positive allosteric modulator enhances the effects of nicotinic agonists.

Calcium Flux Assays using Cells Expressing nAChR Subtypes [00574] Experimental Procedure: Human embryonic kidney (HEK) 293 cells stably expressing human a4(32 or a3134 combinations are grown to confluency in 162 cm2 tissue culture flasks in DMEM media supplemented with 10% FBS and 25 g/ml zeocin and g/ml hygromycin B. IMR-32 neuroblastoma cells (ATCC) are grown to confluency in 162 cm2 tissue culture flasks in minimum essential media supplemented with 10% FBS
and 1 mM
sodium pyruvate, 1 % non-essential amino acids and 1% antibiotic-antimycotic.
The cells are then dissociated using cell dissociation buffer and 100-150 l per well of 3.5 x 105 cells/ml cell suspension (50,000 -100,000 cells/well) was plated into 96-well black plates (poly-D-lysine precoated) with clear bottom and maintained for 24-48 hours in a tissue culture incubator at 37 C under an atmosphere of 5% C02: 95% air. Other clonal cell lines or primary cell cultures that express endogenous a4* nicotinic receptors may also be used in this assay. Calcium flux was measured using calcium-3 assay kit (Molecular Devices, Sunnyvale, CA) or fluo-4 (Invitrogen). A stock solution of the dye was prepared by dissolving each vial supplied by the vendor in Hank's balanced salt solution buffer (HBSS) or 150 mM NMDG, 20 mM CaC12 containing 10 mM HEPES, The stock solution was diluted 1:20 using the same buffer before use. The growth media was removed from the cells. The cells were loaded with 100 l of the dye per well and incubated at room temperature for up to one hour for HEK 293 clonal stable cell lines or 30 minutes - 45 minutes at 37 C for IMR-32 cells Fluorescence measurements were read simultaneously from all the wells by a Fluorometic Imaging Plate Reader (FLIPR) at an excitation wavelength of 480 nm and an emission wavelength of 520 nm. Baseline fluorescence was measured for the first 6 seconds at which 3X concentrations of modulator/test compounds were added to the cell plate at 50 l and incubated for five minutes. The fluorescence intensity was captured every second for the first 1 minute followed by every 5 seconds for an additional 4 minutes. This procedure was followed by 50 l of 4X concentration of agonist and readings were taken for a period of 3-5 minutes as described above. Data was normalized to maximal responses and plotted as a function of concentration. The concentration dependence of changes fluorescence responses was fitted by nonlinear regression analysis (GraphPad Prism, San Diego, CA) to obtain EC50 values.

[00575] The positive allosteric modulator effects on a4(32nAChRs exemplified by 3-(3-pyridin-3-yl-1,2,4-oxadiazol-5-yl)benzonitrile (Compound 1) and 3,5-di(pyridin-3-yl)-1,2,4-oxadiazole (Compound 2) can be identified by measuring their potentiating effect to fluorescence changes in intracellular calcium using a fluorimetric plate reader. The potentiating effect of an a4(32modulator on a4(32receptor can also be illustrated by concentration responses to a4(32 agonists, for example 5-[(2R)-azetidin-2-ylmethoxy]-2-chloropyridine (Compound A) and (3R)-1-pyridin-3-ylpyrrolidin-3-amine (Compound B), in presence of a fixed concentration of PAM. As shown in Figures IA and 2A, in the presence of an a4(32PAM (for example, 3-(3-(pyridin-3-yl)-1,2,4-oxadiazol-5-yl)benzonitrile (Compound 1) at 10 M), the concentration-responses to a4(32agonists, for example 5-[(2R)-azetidin-2-ylmethoxy] -2-chloropyridine (Compound A) and (3R)-1-pyridin-3-ylpyrrolidin-3-amine (Compound B), are shifted typically by 1 - 2 log units (10-100-fold) to the left resulting in more potent EC50 values to agonists. In addition to compound A
and B, other known nicotinic agonists can be left-shifted in presence of a4(32 PAM such as 3-(3-(pyridin-3-yl)-1,2,4-oxadiazol-5-yl)benzonitrile (Compound 1, Figure 2C). When these experiments are done with cells expressing other nAChR subunits such as a3134 (see Figures lB and 2B), the PAM is unable to affect the concentration responses to the agonists. This shows that PAMs can selective enhance potency of the compound selectively at a4(32, but not other (e.g.
a3134) subtypes. This could lead to preferential effects of the agonist at the desired subtype, viz., a4(32, without effects at other nicotinic receptor subtypes and thus enhancing in vivo selectivity of the agonist.
[00576] Table 1 lists the results for the compounds of the present invention.
The activity (allosteric effects - potentiation of fluorescence responses) ranges are defined as follows; "a"
denotes as activity range from 200 - 400%, "b" denotes an activity range from 150-200%, "c" denotes an activity range from 120-150% and "d" denotes an activity range 90-120%.
Table 1. Examples of Selected a4(32 positive allosteric modulators Example No. Structure Activity Example Structure Activity N-O CN N-O
1 ~N a 19 N b N-O N-O SOZNHz 2 `N / a 35 a N N N

NC N-O N-O NO, 3 N / a 40 N a ~N N

4 \ I ~N F b 41 C J ~N , j 2 c N N
/
N-O Br CI N-O
5 ~N 1 c 42 N N c N

N
N-O CN N-0) N
NH
7 /N b 52 N d N
N
N-O OH N-O

9 \ d 55 O 1 c H N
N-O F N-O _ N F a 68 N Q b N N
O
NC N-O
N-O F F
11 N a 76 N CI b /

N'N
N O F
125 N a 83 N a ~O F
N,O/ N,0 N
II F N_ O-N
101 N N a 132 N N a N,0 - F / 0 O

[00577] Example B: a4(32Positive allosteric modulator enhances the effects of nicotinic ligands with very low intrinsic agonist efficacy.

[00578] Calcium Flux Assays: HEK-293 cells stably expressing human a4(32 or a3134 are 5 to confluency in 162 cm2 tissue culture flasks in DMEM media supplemented with 10% FBS
and 25 g/ml zeocin and 200 g/ml hygromycin B. IMR-32 neuroblastoma cells (ATCC) are grown to confluency in 162 cm2 tissue culture flasks in minimum essential media supplemented with 10% FBS and 1 mM sodium pyruvate, 1 % non-essential amino acids and I% antibiotic-antimycotic. The cells are then dissociated using cell dissociation buffer and 10 100-150 l per well of 3.5 x 105 cells/ml cell suspension (50,000 -100,000 cells/well) was plated into 96-well black plates (poly-D-lysine precoated) with clear bottom and maintained for 24-48 hours in a tissue culture incubator at 37 C under an atmosphere of 5% C02: 95%
air. Other clonal cell lines or dissociated primary cortical neurons that express endogenous a4* nicotinic receptors may also be used in this assay. Calcium flux was measured using calcium-3 assay kit (Molecular Devices, Sunnyvale, CA) or fluo-4 (Invitrogen).
A stock solution of the dye was prepared by dissolving each vial supplied by the vendor in Hank's balanced salt solution buffer (HBSS) or 150 mM NMDG, 20 mM CaClz containing 10 mM
HEPES. The stock solution was diluted 1:20 using the same buffer before use.
The growth media was removed from the cells. The cells were loaded with 100 l of the dye per well and incubated at room temperature for up to one hour for HEK 293 clonal stable cell lines or 30 minutes - 45 minutes at 37 C for IMR-32 cells. Fluorescence measurements were read simultaneously from all the wells by a Fluorometic Imaging Plate Reader (FLIPR) at an excitation wavelength of 480 nm and an emission wavelength of 520 nm. Baseline fluorescence was measured for the first 6 seconds at which 3X concentrations of modulator/test compounds were added to the cell plate at 50 l and incubated for five minutes. The fluorescence intensity was captured every second for the first 1 minute followed by every 5 seconds for an additional 4 minutes. This procedure was followed by 50 l of 4X concentration of agonist and readings were taken for a period of 3-5 minutes as described above. Data was normalized to maximal responses and plotted as a function of concentration. The concentration dependence of changes fluorescence responses was fitted by nonlinear regression analysis (GraphPad Prism, San Diego, CA) to obtain EC50 values.
[00579] a4(32 PAMs can also enhance the efficacy of partial agonists (compounds that bind, but activate a4(32 nAChRs with low intrinsic efficacy leading to otherwise barely detectable effects on calcium responses). For example, responses to 2-methyl-3-(2-(S)-pyrrolidinylmethoxy)pyridine (Compound C) in the presence and absence of PAM
is shown in Figure 3. The results show in the presence of an a4(32 PAM (for example, 3-(3-(pyridin-3-yl)-1,2,4-oxadiazol-5-yl)benzonitrile (Compound 1)), the maximum fluorescence calcium signal was substantially enhanced to application of 2-methyl-3-(2-(S)-pyrrolidinylmethoxy)pyridine at the a4(32 receptor (Figure 3A), but again, not at the a3134 receptor (Figure 3B). Another example is provided by Compound D, (1S,5S)-3-(3,6-diaza-bicyclo[3.2.0]hept-3-yl)-quinoline; compound with toluene-4-sulfonic acid which also binds to a4(32 nAChR ([3H]cyt Ki = 6 nM), but does not show substantial calcium responses alone;
however, when co-incubated with a4(32 PAM, responses are substantially enhanced at a4(32 nAChR (Figure 4A), but not at a3134 nAChRs (Figure 4B). These observations provides mechanistic support for compounds like 2-methyl-3-(2-(S)-pyrrolidinylmethoxy)pyridine and (1S,5S)-3-(3,6-Diaza-bicyclo[3.2.0]hept-3-yl)-quinoline being more effective when co-applied with the PAM. The potentiation of a4(32 effects by PAM may potential for optimizing and enhancing efficacy in indications such as ADHD, cognitive deficits, Alzhiemer's disease, and pain.

[00580] Figure 5 shows a comparison of EC50 values from calcium fluorescence (FLIPR) assays using a4(32 nAChRs of several nicotinic agonists including varenicline and ispronicline in the presence and absence of positive allosteric modulator. The potency (EC50 values) of the nicotinic agonists increase in the presence of the positive allosteric modulator.

[00581 ] Example C: a4(32 PAM enhances the effiacy of Compound A in an in vivo model of neuropathic pain.

[00582] To assess whether a4(32 PAM can increase antinociceptive responses of agonists in vivo, the following study was conducted. The materials and methods used to accomplish the study follow.
[00583] Animals: Male Sprague-Dawley rats (Charles River, Wilmington, MA) weighing 120-150 grams at time of surgery were utilized. These animals were group housed in AAALAC approved facilities at Abbott Laboratories in a temperature-regulated environment with lights on between 0700 and 2000 hours. Food and water was available ad libitum except during testing. All animal handling and experimental protocols were approved by an institutional animal care and use committee (IACUC). All experiments were performed during the light cycle.
[00584] Chemicals: 5-[(2R)-Azetidin-2-ylmethoxy]-2-chloropyridine (Compound A, 100 nmol/kg) and 3-(3-pyridin-3-yl)-1,2,4-oxadiazol-5-yl)benzonitrile (a4(32 PAM

Compound 1, 1-35 mol/kg, i.p.) was used. Compound A and D was prepared in saline and injected in solution in a volume of 2 ml/kg body weight 30 minutes before behavioral evaluation. Compound 1, 3-(3-(pyridin-3-yl)-1,2,4-oxadiazol-5-yl)benzonitrile, was prepared in 30% hydroxybetacyclodextrin and injected in solution in a volume of 4 ml/kg body weight immediately before Compound A. For studies with Compound D, the doses tested ranged from 0.3 - 30 mol/kg i.p.
[00585] Experimental Procedure: To produce neuropathic pain, tight ligation of the L5-L6 spinal nerves was performed. As previously described in detail by Kim and Chung (Kim SH
and Chung JM (1992), Pain 50: 355), following sterilization procedures, a 1.5 cm incision was made dorsal to the lumbosacral plexus. The paraspinal muscles (left side) were separated from the spinous processes, the L5 and L6 spinal nerves isolated, and tightly ligated with 3-0 silk thread. Following hemostasis, the wound was sutured and coated with antibiotic ointment. The rats were allowed to recover and then placed in a cage with soft bedding for 7-14 days before behavioral testing for mechanical allodynia.

[00586] Tactile allodynia was measured using calibrated (force; g) von Frey filaments (Stoelting, Wood Dale, IL). Briefly, rats were placed into individual plexiglass containers and allowed to acclimate for 15-20 minutes before testing. Withdrawal threshold was determined by increasing and decreasing stimulus intensity and estimated using a Dixon non-parametric test (Chaplan et al., 1994; Chaplan SR, Bach FW, Pogrel JW, Chung JM and Yaksh TL (1994) J Neurosci Methods 53:55-63). Only rats with threshold scores < 4.5 g were considered allodynic and utilized in further testing. A percent of maximal possible effect (% M.P.E.) of the tested compounds was calculated according to the formula: ([post-drug threshold] - [baseline threshold])/([maximum threshold] - [baseline threshold]) x 100%, where maximum threshold was equal to 15 g.
[00587] Statistical analysis: Analysis of the in vivo data was carried out using analysis of variance. Where appropriate, Bonferroni's Multiple Comparison Test was used for post-hoc analysis. The level of significance was set at p less than 0.05. Data are presented as mean S.E.M.
[00588] Results: As shown in Figure 6A, tight ligation of L5-L6 spinal nerves induced pronounced mechanical allodynia with a decrease in mechanical paw withdrawal threshold (PWT) in the vehicle group to 2.6 0.4g. Compound 1, 3-(3-(pyridin-3-yl)-1,2,4-oxadiazol-5-yl)benzonitrile (PAM,10 mg/kg, i. p.) did not produce significant reversal of nerve injury induced mechanical allodynia (PWT: 3.3 0.4g, P greater than 0.05 vs. vehicle group).

Compound A (0.03 mol/kg, i.p.) produced weak but significant reversal of mechanical allodynia (PWT: 5.6 0.3g, P less than 0.001 vs. vehicle group). When co-administered, Compound A + 3-(3-(pyridin-3-yl)-1,2,4-oxadiazol-5-yl)benzonitrile (Compound 1, PAM) produced a pronounced reversal of nerve injury-induced mechanical allodynia (PWT: 12.1 0.5g) that was significantly different from vehicle (P less than 0.001), but also from Compound A alone (P less than 0.001) and 3-(3-(pyridin-3-yl)-1,2,4-oxadiazol-5-yl)benzonitrile, Compound 1, alone (P less than 0.001). This study demonstrates that the co-administration of a a4(32 positive allosteric modulator to a a4(32 agonist potentiate the antiallodynic effects of the agonist. Since the efficacy of the a4(32 ligand in neuropathic pain is robustly improved in presence of a PAM, an overall improvement in therapeutic window for the treatment of pain may be envisaged utilizing a combination approach (agonist in combination with the a4(32 PAM).
[00589] Figure 6B shows that the effects of PAM (3-(3-(pyridin-3-yl)-1,2,4-oxadiazol-5-yl)benzonitrile, Compound 1) are dose-dependent. An ineffective dose of Compound A (1 nmol/kg), when combined with varying doses of PAM (3 -(3 -(pyridin-3 -yl)-1,2,4-oxadiazol-5-yl)benzonitrile, Compound 1) results in dose-dependent increase in efficacy, approaching at least that of gabapentin, a drug clinically used for the treatment of neuropathic pain.
[00590] Figure 7A shows dose dependent effects in neuropathic pain of 5-[(2R)-azetidin-2-ylmethoxy]-2-chloropyridine (Compound A) alone, a4(32 PAM (3-(3-(pyridin-3-yl)-1,2,4-oxadiazol-5-yl)benzonitrile, Compound 1) alone and a combination of Compound 1 (3.5 mol/kg) with various doses of Compound A. a4(32 PAM (Compound 1) alone is ineffective, but is capable of left-shifting the dose response curve of Compound A in the Chung model of neuropathic pain.

[00591 ] Example D: Analysis of compound effects on emesis in ferrets.
[00592] Fasted male ferrets (Marshall BioResources, North Rose, NY) weighing between 1.0 and 1.7 kg are used to determine the emetic effects. a4(32 PAM (Compound 1) was administered first and thirty minutes later, Compound A was administered at various doses.
After dosing, the animals were observed for emesis and behaviors characteristic of nausea for a period of 90 minutes. The percentage of animals that experienced emesis at a given dose was recorded.
[00593] Figure 7B shows effects on emesis. Shown are effects of 5-[(2R)-azetidin-2-ylmethoxy]-2-chloropyridine (Compound A) alone, a4(32 PAM (Compound 1) alone and a combination of Compound 1 (3.5 mol/kg) with various doses of compound A.
a4(32 PAM

(Compound 1) alone does not cause emesis, and does not shift the dose response curve of Compound A in the ferret model of emesis.
[00594] Figures 8A and 8B show plasma level analysis in models of neuropathic pain and emesis. Note the left ward shift in efficacy of Compound A in Figure 8A, but no shift in effects on emesis in Figure 8B. In other words, maximal efficacy of Compound A
can be realized in neuropathic pain without incidence of emesis, in presence of a4(32 PAM
(Compound 1), thus widening the therapeutic window of a4132 nAChR agonists [00595] Example E: a4(32 Partial agonists can be effective in reversing neuropathic pain in the presence of a4(32 positive allosteric modulators [00596] To further examine effects in neuropathic pain, the effects of Compound D, another a4(32 ligand with low intrinsic efficacy (partial agonist) was examined in the Chung model. Alone, Compound D is ineffective in reversing neuropathic pain, but when combined with the PAM (Compound 1), significant efficacy can be realized. Figure 9 shows the efficacy of partial agonist, Compound D in the presence and absence of a4132 PAM
(Compound 1). Compound D when administered alone is ineffective in relieving pain. When co-dosed with a4(32 PAM (Compound 1), Compound D is now effective, and shows significant relief of neuropathic pain in rats. As shown previously, PAM
(Compound 1) alone is ineffective (P+V).

[00597] Characterization of Nicotinic Acetylcholine Receptor Ligands _ [00598] In addition to the assays previously described for assessing nicotinic acetylcholine receptor positive allosteric modulators (fluroscence-based measurements, electrophysiology measurements using Xenopus oocytes or cell lines), the receptor interactions of positive allosteric modulators at a4(32 nAChRs also can be evaluated according to the [3H]-POB
binding assay, which was performed as described below.

[00599] [3H]-3-(5-(Pyridin-3-yl)-1,2,4-oxadiazol-3-yl)benzonitrile ([3H]-POB) Binding [00600] [3H]-POB ([3H]-3-(5-(pyridin-3-yl)-1,2,4-oxadiazol-3-yl)benzonitrile) binding to a a4(32nAChR modulator site was determined using membrane enriched fractions from human cortex (ABS Inc., Wilmington, DE). Pellets were thawed at 4 C, washed and resuspended with a Polytron at a setting of 7 in 30 volumes of BSS-Tris buffer (120 mM
NaCl, 5 mM
KC1, 2 mM CaC12, 2 mM MgC12, and 50 mM Tris-Cl, pH 7.4, 4 C). For saturation binding isotherms, eight concentrations of [3H]-POB (10-250 nM) in quadruplicate and homogenate containing 100-200 gg of protein were incubated in a final volume of 500 gL
for 75 minutes at 4 C. Non-specific binding was determined in the presence of 30 gM
unlabeled 3-(5-(pyridin-3-yl)-1,2,4-oxadiazol-3-yl)benzonitrile. Under these conditions, saturable binding of [3H]-POB binding was measured in membrane enriched fractions from human frontal cortex (Figure 10). The Kd and Bmax values were 60 16 nM and 2900 500 fmol/mg protein, respectively. Membrane preparations from other species (rat, mouse, ferret) and from clonal or transfected cell lines that express a4(32 nAChRs cloned from various species may also be used in this binding assay.
[00601] For use in concentration-inhibition assays, seven log-dilution concentrations of test compounds containing 100-200 gg of protein, and 50 nM [3H]-POB (16.4 Ci/mmol) were incubated in a final volume of 500 gL for 75 minutes at 4 C in duplicate. Non-specific binding was determined in the presence of 30 gM 3-(5-(pyridin-3-yl)-1,2,4-oxadiazol-3-yl)benzonitrile. Bound radioactivity was collected on Millipore MultiScreen harvest plates FB presoaked with 0.3% polyethyleneimine using a PerkinElmer cell harvester, washed with 2.5 mL of ice-cold buffer, and radioactivity was determined using a PerkinElmer TopCount Microplate beta counter. Dissociation constant (Kd) and maximum binding (Bmax) values from saturation binding experiments were determined using GraphPad Prism (Graphpad Software, San Diego, CA). IC50 values were determined by nonlinear regression in Microsoft Excel or Assay Explorer. K; values were calculated from the IC50S
using the Cheng-Prusoff equation, where K; = IC50/(1+[Ligand]/KD]).
[00602] [3H]-POB was obtained according to the preparation procedures generally described in Example 135 shown below.

Example 135 [3H]-3-(5-(pyridin-3-yl)-1,2,4-oxadiazol-3-yl)benzonitrile Example 135A
3-(5-(5-bromopyridin-3-yl)-1,2,4-oxadiazol-3-yl)benzonitrile [00603] The title compound was prepared according to the procedure of Example 4B using 3-cyano-N'-hydroxybenzimidamide and 5-bromonicotinoyl chloride (Alfa). 1H NMR
(300 MHz, DMSO-d6) 6 7.67 (m, 1 H), 7.74 (m, 1 H), 8.41 (m, 1 H), 8.49 (m, 1 H), 8.64 (s, 1 H), 8.93 (s, 1 H), 9.4 (s, 1 H) ppm; MS (DCI/NH3) m/z 327 (M+H)+.
Example 135B
[3H1-3-(5-(pyridin-3-yl)-1,2,4-oxadiazol-3-yl)benzonitrile ([3H1-POB) [00604] The compound of Example 135A was dissolved in a mixture of dichloromethane, triethylamine, and 5% palladium on carbon. The reaction solution was then saturated with tritium gas (1.2 Ci). The reaction mixture was stirred at room temperure for 3.5 hours, the catalyst was removed by filtration, ant the filtrate was concentrated to yield crude tritiated product. Further purification of the crude material by reverse-phase HPLC
using a 30 minute 40% isocratic acetonitrile run (column LunaC 18, 254 nm) to provide a total of 200 mCi (1 mL, methanol).
[00605] The radiochemical purity of [3H]-POB was found to be 99% and the specific activity was determined to be 16.4 Ci/mmol.

[00606] Nicotinic acetylcholine receptor ligands suitable for the invention exhibit K; values ranging about 1 nanomolar to about 10 micromolar when tested by the [3H]-POB
assay, many having a K; of less than 5 micromolar. Compounds that modulate the function of a4(32nAChRs by altering the activity of the receptor or signaling are suitable for the composition. More specifically, the compounds that function as allosteric modulators enhancing the efficacy and/or potency of acetylcholine or a nicotinic agonist are desired.
Multiple binding sites at a4(32 nAChRs may exist for such compounds, of which only one site may be defined by [3H]POB binding.
[00607] Also contemplated is compound of formula(II*):
Ar2N -Ara O-N
(11*), wherein Ar 2 is aryl or heteroaryl, wherein the aryl or heteroaryl is substituted or unsubstituted, and, when substituted, the aryl or heteroaryl is substituted with 1, 2, 3, or 4 substituents selected from halo, Ci-C6 haloalkyl, C6-C10 aryl, C4-C7 cycloalkyl, C2-C6 alkenyl, C2-C6 alkynyl, C5-C10 heteroaryl, C4-C10 heterocycle, Ci-C6 alkyl, -(C1-C6 alkyl)NHC(O)O-(Ci-C6 alkyl), Ci-C6 hydroxyalkyl, Ci-C6 alkylcarbonyl, amino, hydroxyl, haloalkyl-C(O)-, haloalkyl-S02-, alkyl-S02-, -SO2NH2, -SO2NH(CI-C6 alkyl), -SO2N(C1-C6 alkyl)2, cyano, nitro, Ci-C6 acylamino, Ci-C6 alkoxy, -C(O)NH2, -C(0)0-(CI-C6 alkyl), and carboxy; and [00608] Ar 3 is aryl or heteroaryl, wherein the aryl or heteroaryl is substituted or unsubstituted, and, when substituted, the aryl or heteroaryl is substituted with a substituent selected from halo, Ci-C6 haloalkyl, C6-C10 aryl, C4-C7 cycloalkyl, C2-C6 alkenyl, C2-C6 alkynyl, C5-C10 heteroaryl, Ci-C6 alkyl, Ci-C6 hydroxyalkyl, amino, hydroxyl, haloalkyl-S02-, cyan, nitro, Ci-C6 acylamino, Ci-C6 alkoxy, -N(Ci-C6 alkyl)2, and carboxy;
wherein at least one of the available atoms within a compound of formula (11*) is replaced with a radioisotope. A particular radiolabelled compound of formula (11*) is [3H]-3-(5-(pyridin-3-yl)-1,2,4-oxadiazol-3-yl)benzonitrile. Such compounds are suitable for use in determining the binding affinity of nicotinic acetylcholine receptor subtype a4(32 positive allosteric modulators.
[00609] Another embodiment of the invention is a radiolabeled compound of formula (11*), wherein Ar 2 and Ara are independently phenyl, pyrazinyl, pyridinyl, pyrimidinyl, pyridazine, triazinyl, or a bicyclic heteroaryl, substituted independently with 0, 1, 2, 3, or 4 substitutents selected from the group consisting of alkoxy, alkoxycarbonyl, alkoxycarbonylamino, alkoxycarbonylaminoalkyl, alkyl, alkylamino, alkylcarbonyl, alkylsulfonyl, amido, amino, aminoalkyl, carboxy, dialkylamino, dialkylaminoalkyl, halo, haloalkyl, haloalkylcarbonyl, heteroaryl, heterocycle, hydroxy, hydroxyalkyl, cyan, nitro, sulfonamide and dialkylsulfonylformimidamide; provided that when one of Ar 2 and Ar 3 is pyridinyl or aryl, the other is not pyridinyl; when Ar 3 is pyridinyl, Ar 2 is not pyrazinyl.
[00610] It is understood that the foregoing detailed description and accompanying examples are merely illustrative and are not to be taken as limitations upon the scope of the invention, which is defined solely by the appended claims and their equivalents. The compositions, methods, and articles of manufacture have been described with reference to various specific embodiments and techniques. However, various changes and modifications, including without limitation those relating to the compounds, substituents, syntheses, and/or methods of use of the invention, may be made without departing from the spirit and scope thereof. The examples described herein are intended only to illustrate and do not limit the scope of the invention as defined in the appended claims and equivalents thereof.

Claims (20)

WHAT IS CLAIMED IS:
1. A compound of formula (II):

wherein Ar2 and Ar3 are independently, optionally substituted aryl or heteroaryl.
2. A compound of of formula (II):

wherein Ar2 and Ar3 are independently aryl, pyrazinyl, pyridazinyl, pyridinyl, pyrimidinyl, triazinyl, or bicyclic heteroaryl, substituted independently with 0, 1, 2, 3, or 4 substitutents selected from the group consisting of alkoxy, alkoxycarbonyl, alkoxycarbonylamino, alkoxycarbonylaminoalkyl, alkyl, alkylamino, alkylcarbonyl, alkylsulfonyl, amido, amino, aminoalkyl, carboxy, dialkylamino, dialkylaminoalkyl, halo, haloalkyl, haloalkylcarbonyl, heteroaryl, heterocycle, hydroxy, hydroxyalkyl, cyano, nitro, oxo, sulfonamide and dialkylsulfonylformimidamide; provided that when one of Ar2 and Ar3 is pyridinyl or aryl, the other is not pyridinyl; when Ar3 is pyridinyl, Ar2 is not pyrazinyl;
or a pharmaceutically acceptable salt thereof.
3. The compound of claim 2 or a pharmaceutically acceptable salt thereof, wherein Ar2 is phenyl.
4. The compound of claim 2 or a pharmaceutically acceptable salt thereof, wherein Ar2 is pyridinyl.
The compound of claim 2 or a pharmaceutically acceptable salt thereof, wherein Ar2 is bicyclic heteroaryl.
6. The compound of claim 2 or a pharmaceutically acceptable salt thereof, wherein Ar3 is pyrimidinyl or pyridazinyl.
7. The compound of claim 2 or a pharmaceutically acceptable salt thereof, wherein Ar3 is bicyclic heteroaryl.
8. The compound of claim 2 or a pharmaceutically acceptable salt thereof, wherein Ar2 and Ar3 are independentlyphenyl, pyridazinyl, pyridinyl, pyrimidinyl, or bicyclic heteroaryl.
9. A compound of claim 2, selected from the group consisting of:
5-(2,3-difluorophenyl)-3-(pyrimidin-5-yl)-1,2,4-oxadiazole;
5-(pyridin-3-yl)-3-(pyrimidin-5-yl)-1,2,4-oxadiazole;
2-fluoro-N,N-dimethyl-4-(3-(pyrimidin-5-yl)-1,2,4-oxadiazol-5-yl)aniline;
3-(3-(pyrimidin-5-yl)-1,2,4-oxadiazol-5-yl)benzonitrile;
5-(3,4-difluorophenyl)-3-(pyridazin-4-yl)-1,2,4-oxadiazole;
3-(pyridazin-4-yl)-5-(pyridin-3-yl)-1,2,4-oxadiazole;
N,N-dimethyl-N'-(4-(3-(pyrimidin-5-yl)-1,2,4-oxadiazol-5-yl)phenylsulfonyl)formimidamide;
5-(4-fluorophenyl)-3-(pyrimidin-5-yl)-1,2,4-oxadiazole;
5-(3-fluorophenyl)-3-(pyrimidin-5-yl)-1,2,4-oxadiazole;
3-(pyrimidin-5-yl)-5-(3,4,5-triluorophenyl)-1,2,4-oxadiazole;
5-(2-chloropyridin-4-yl)-3-(pyrimidin-5-yl)-1,2,4-oxadiazole;
3-(Pyridin-3-yl)-5-(pyrimidin-5-yl)-1,2,4-oxadiazole;
5-(Pyridazin-4-yl)-3-(pyridin-3-yl)-1,2,4-oxadiazole;
3-(3-(pyridazin-4-yl)-1,2,4-oxadiazol-5-yl)benzonitrile;
5-(3-fluorophenyl)-3-(pyridazin-4-yl)-1,2,4-oxadiazole;
3-(pyridazin-4-yl)-5-(3,4,5-trifluorophenyl)-1,2,4-oxadiazole;
5-(3,5-difluorophenyl)-3-(pyridazin-4-yl)-1,2,4-oxadiazole;
5-(4-fluorophenyl)-3-(pyridazin-4-yl)-1,2,4-oxadiazole;
3-(pyridazin-4-yl)-5-(pyrimidin-5-yl)-1,2,4-oxadiazole;
3-(pyridazin-4-yl)-5-(2,3,6-trifluorophenyl)-1,2,4-oxadiazole;
3-(pyridazin-4-yl)-5-(2,3,4-trifluorophenyl)-1,2,4-oxadiazole;

N,N-dimethyl-N'-(4-(3-(pyridazin-4-yl)-1,2,4-oxadiazol-5 yl) phenylsulfonyl) formimidamide;
5-(3,4-difluorophenyl)-3-(pyrimidin-5-yl)-1,2,4-oxadiazole;
3-(3,4-difluorophenyl)-5-(pyrimidin-5-yl)-1,2,4-oxadiazole;
3-(pyrimidin-5-yl)-5-(2,3,4-trifluorophenyl)-1,2,4-oxadiazole;
3-(pyrimidin-5-yl)-5-(2,3,6-trifluorophenyl)-1,2,4-oxadiazole;
3-(pyrimidin-5-yl)-5-(2,3,4,5-tetrafluorophenyl)-1,2,4-oxadiazole;
5-(imidazo [ l ,5-a]pyridin-6-yl)-3-(pyridin-3-yl)-1,2,4-oxadiazole;
5-(1H-indol-6-yl)-3-(pyridin-3-yl)-1,2,4-oxadiazole;
5-(2,7-dimethylpyrazolo [ 1,5-a]pyrimidin-6-yl)-3-(pyridin-3-yl)-1,2,4-oxadiazole;
5-(2,2-difluorobenzo [d] [ 1,3 ]dioxol-5-yl)-3-(pyridin-3-yl)-1,2,4-oxadiazole;
5-(2-methylbenzofuran-5-yl)-3-(pyridin-3-yl)-1,2,4-oxadiazole;
5-(benzo [d] [ 1,2,3 ]thiadiazol-5-yl)-3-(pyridin-3-yl)-1,2,4-oxadiazole;
5-(1H-benzo[d]imidazol-5-yl)-3-(pyridin-3-yl)-1,2,4-oxadiazole;
5-(1H-benzo[d] [1,2,3]triazol-5-yl)-3-(pyridin-3-yl)-1,2,4-oxadiazole;
5-(benzo[d]thiazol-5-yl)-3-(pyridin-3-yl)-1,2,4-oxadiazole;
3-(pyridin-3-yl)-5-(1 H-pyrrolo [2,3-b]pyridin-5-yl)-1,2,4-oxadiazole;
5-(1H-indol-5-yl)-3-(pyridin-3-yl)-1,2,4-oxadiazole;
5-(benzofuran-5-yl)-3-(pyridin-3-yl)-1,2,4-oxadiazole;
5-(1-methyl-1 H-benzo [d]imidazol-5-yl)-3-(pyridin-3-yl)-1,2,4-oxadiazole;
3-(imidazo [ 1,2-a]pyridin-6-yl)-5-(pyridin-3-yl)-1,2,4-oxadiazole;
5-(6-chloropyridin-3-yl)-3-(imidazo [ 1,2-a]pyridin-6-yl)-1,2,4-oxadiazole;
5-(6-fluropyridin-3-yl)-3-(imidazo[1,2-a]pyridin-6-yl)-1,2,4-oxadiazole;
5-(5-fluropyridin-3-yl)-3-(imidazo[1,2-a]pyridin-6-yl)-1,2,4-oxadiazole;
5-(1H-indazol-5-yl)-3-(pyridin-3-yl)-1,2,4-oxadiazole;
5-([ 1,2,4]triazolo [4,3-a]pyridin-6-yl)-3-(pyridin-3-yl)-1,2,4-oxadiazole;
5-(3-(pyridin-3-yl)-1,2,4-oxadiazol-5-yl)benzo[d]oxazol-2(3H)-one;
5-(3-(pyridin-3-yl)-1,2,4-oxadiazol-5-yl)-1 H-benzo [d]imidazole-2(3H)-thione;

1,3-dimethyl-5-(3-(pyridin-3-yl)-1,2,4-oxadiazol-5-yl)-1 H-benzo [d]imidazol-2(3H)-one;
6-(3-(pyridin-3-yl)-1,2,4-oxadiazol-5-yl)benzo[d]oxazol-2(3H)-one;
5-(3-(pyridin-3-yl)-1,2,4-oxadiazol-5-yl)-1 H-benzo [d]imidazol-2(3H)-one;
6-(3-(Pyridin-3-yl)-1,2,4-oxadiazol-5-yl)benzo[d]oxazol-2-amine;
6-(3-(pyridin-3-yl)-1,2,4-oxadiazol-5-yl)benzo[d]oxazole;

5-(5-(pyridin-3-yl)-1,2,4-oxadiazol-3-yl)benzo[d]oxazol-2(3H)-one;
5-(5-(6-chloropyridin-3-yl)-1,2,4-oxadiazol-3-yl)benzo[d]oxazol-2(3H)-one; and -(benzo [d] [ 1,3 ] dioxol-5-yl)-3-(pyridin-3-yl)-1,2,4-oxadiazole;
or a pharmaceutically acceptable salt thereof.
10. The compound of claim 2, selected from the group consisting of 5-(3-fluorophenyl)-3-(pyridazin-4-yl)-1,2,4-oxadiazole;
5-(3,4-difluorophenyl)-3-(pyridazin-4-yl)-1,2,4-oxadiazole, 3-(pyridazin-4-yl)-5-(3,4,5-trifluorophenyl)-1,2,4-oxadiazole;
5-(3,5-difluorophenyl)-3-(pyridazin-4-yl)-1,2,4-oxadiazole;
5-(4-fluorophenyl)-3-(pyridazin-4-yl)-1,2,4-oxadiazole;
3-(pyridazin-4-yl)-5-(2,3,6-trifluorophenyl)-1,2,4-oxadiazole; and 3-(pyridazin-4-yl)-5-(2,3,4-trifluorophenyl)-1,2,4-oxadiazole;
or a pharmaceutically acceptable salt thereof.
11. The compound of claim 2, selected from the group consisting of 5-(2-chloropyridin-4-yl)-3-(pyrimidin-5-yl)-1,2,4-oxadiazole;
5-(2,3-difluorophenyl)-3-(pyrimidin-5-yl)-1,2,4-oxadiazole;
5-(3-fluorophenyl)-3-(pyrimidin-5-yl)-1,2,4-oxadiazole;
3-(pyrimidin-5-yl)-5-(3,4,5-triluorophenyl)-1,2,4-oxadiazole 5-(4-fluorophenyl)-3-(pyrimidin-5-yl)-1,2,4-oxadiazole;
5-(3,4-difluorophenyl)-3-(pyrimidin-5-yl)-1,2,4-oxadiazole;
3-(pyrimidin-5-yl)-5-(2,3,4-trifluorophenyl)-1,2,4-oxadiazole;
3-(pyrimidin-5-yl)-5-(2,3,6-trifluorophenyl)-1,2,4-oxadiazole; and 3-(pyrimidin-5-yl)-5-(2,3,4,5-tetrafluorophenyl)-1,2,4-oxadiazole;
or a pharmaceutically acceptable salt thereof.
12. The compound of claim 2, selected from the group consisting of 5-(imidazo [ 1,5-a]pyridin-6-yl)-3-(pyridin-3-yl)-1,2,4-oxadiazole;
5-(1H-indol-6-yl)-3-(pyridin-3-yl)-1,2,4-oxadiazole;
5-(2,7-dimethylpyrazolo [ 1,5-a]pyrimidin-6-yl)-3-(pyridin-3-yl)-1,2,4-oxadiazole;
5 -(2,2-difluorobenzo [d] [ 1,3 ]dioxol-5-yl)-3-(pyridin-3-yl)-1,2,4-oxadiazole;
5-(2-methylbenzofuran-5-yl)-3-(pyridin-3-yl)-1,2,4-oxadiazole;
5-(benzo [d] [ 1,2,3 ]thiadiazol-5-yl)-3-(pyridin-3-yl)-1,2,4-oxadiazole;

5-(1H-benzo[d]imidazol-5-yl)-3-(pyridin-3-yl)-1,2,4-oxadiazole;
5-(1H-benzo[d] [ 1,2,3]triazol-5-yl)-3-(pyridin-3-yl)- 1,2,4-oxadiazole;
5-(benzo[d]thiazol-5-yl)-3-(pyridin-3-yl)-1,2,4-oxadiazole;
3-(pyridin-3-yl)-5-(1 H-pyrrolo [2,3 -b]pyridin-5 -yl)- 1,2,4-oxadiazole;
5-(1H-indol-5-yl)-3-(pyridin-3-yl)-1,2,4-oxadiazole;
5-(benzofuran-5-yl)-3-(pyridin-3-yl)-1,2,4-oxadiazole;
5-(1-methyl-1 H-benzo [d]imidazol-5-yl)-3-(pyridin-3-yl)-1,2,4-oxadiazole;
5-(1H-indazol-5-yl)-3-(pyridin-3-yl)-1,2,4-oxadiazole;
-([1,2,4]triazolo [4,3 -a]pyridin-6-yl)-3 -(pyridin-3 -yl)- 1,2,4-oxadiazole;
5-(3-(pyridin-3-yl)-1,2,4-oxadiazol-5-yl)benzo[d]oxazol-2(3H)-one;
5-(3-(pyridin-3-yl)-1,2,4-oxadiazol-5-yl)-1 H-benzo [d]imidazole-2(3H)-thione;

1,3-dimethyl-5-(3-(pyridin-3-yl)-1,2,4-oxadiazol-5-yl)-1 H-benzo [d]imidazol-2(3H)-one;
6-(3-(pyridin-3-yl)-1,2,4-oxadiazol-5-yl)benzo[d]oxazol-2(3H)-one;
5-(3-(pyridin-3-yl)-1,2,4-oxadiazol-5-yl)-1 H-benzo [d]imidazol-2(3H)-one;
6-(3-(pyridin-3-yl)-1,2,4-oxadiazol-5-yl)benzo[d]oxazol-2-amine;
6-(3-(pyridin-3-yl)-1,2,4-oxadiazol-5-yl)benzo[d]oxazole; and 5 -(benzo [d] [ 1,3 ] dioxol-5-yl)-3-(pyridin-3-yl)-1,2,4-oxadiazole;
or a pharmaceutically acceptable salt thereof.
13. The compound of claim 2, selected from the group consisting of 3-(imidazo [ 1,2-a]pyridin-6-yl)-5-(pyridin-3-yl)-1,2,4-oxadiazole;
5-(6-chloropyridin-3-yl)-3-(imidazo [ 1,2-a]pyridin-6-yl)-1,2,4-oxadiazole;
5-(6-fluropyridin-3-yl)-3-(imidazo[1,2-a]pyridin-6-yl)-1,2,4-oxadiazole;
5-(5-fluropyridin-3-yl)-3-(imidazo[1,2-a]pyridin-6-yl)-1,2,4-oxadiazole;
5-(5-(pyridin-3-yl)-1,2,4-oxadiazol-3-yl)benzo[d]oxazol-2(3H)-one; and 5-(5-(6-chloropyridin-3-yl)-1,2,4-oxadiazol-3-yl)benzo[d]oxazol-2(3H)-one;
or a pharmaceutically acceptable salt thereof.
14. A radiolabled compound of formula (11*):
wherein Ar2 and Ar3 are independently aryl, pyrazinyl, pyridazinyl, pyrimidinyl, triazinyl, or a bicyclic heteroaryl substituted independently with 0, 1, 2, 3, or 4 substitutents selected from the group consisting of alkoxy, alkoxycarbonyl, alkoxycarbonylamino, alkoxycarbonylaminoalkyl, alkyl, alkylamino, alkylcarbonyl, alkylsulfonyl, amido, amino, aminoalkyl, carboxy, dialkylamino, dialkylaminoalkyl, halo, haloalkyl, haloalkylcarbonyl, heteroaryl, heterocycle, hydroxy, hydroxyalkyl, cyano, nitro, sulfonamide and dialkylsulfonylformimidamide; provided that when one of Ar2 and Ar3 is pyridinyl or aryl, the other is not pyridinyl; when Ar3 is pyridinyl, Ar2 is not pyrazinyl;
or a pharmaceutically acceptable salt thereof.
15. A pharmaceutical composition comprising a therapeutically effective amount of a compound of claim 2, or a salt thereof, and a pharmaceutically acceptable carrier or excipient.
16. The composition of claim 15, further comprising a nicotinic acetylcholine receptor ligand.
17. The composition of claim 16, wherein the nicotinic acetylcholine receptor ligand is a nicotinic acetylcholine receptor subtype .alpha.4.beta.2 ligand demonstrating a Ki value that as measured by [3H]-cytisine binding assay (Ki Cyt) of about 0.001 nanomolar to about 100 micromolar.
18. The composition of claim 16, wherein the nicotinic acetylcholine receptor ligand is a nicotinic acetylcholine receptor subtype a4.beta.2 agonist or partial agonist.
19. A method for use in treating or preventing pain in a patient, comprising:
(i) administering a therapeutically effective amount of the compound of claim 2; and (ii) administering a pain medication comprising a compound selected from an opioid, gabapentin, pregabalin, duloxetine, a cannabinoid ligand, a vaniolloid receptor antagonist, calcium channel blocker and a sodium channel blocker wherein a descending modulatory pathway that is shared or commonly activated via the a4.beta.2 nicotinic receptor mechanism is activated.
20. A method for treating or preventing attention deficit disorder, attention deficit hyperactivity disorder (ADHD), Alzheimer's disease (AD), bipolar disorder, mild cognitive impairment, age-associated memory impairment (AAMI), senile dementia, AIDS
dementia, Pick's disease, dementia associated with Lewy bodies, dementia associated with Down's syndrome, schizophrenia, schizoaffective disorder, smoking cessation, substance abuse including alcohol abuse, amyotrophic lateral sclerosis, Huntington's disease, diminished CNS
function associated with traumatic brain injury, or pain, comprising administering a therapeutically effective amount of the compound of claim 2, or a salt thereof, to a subject in need thereof.
CA2725573A 2008-06-06 2008-06-06 Novel 1,2,4 oxadiazole compounds and methods of use thereof Abandoned CA2725573A1 (en)

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
PCT/US2008/066002 WO2009148452A1 (en) 2008-06-06 2008-06-06 Novel 1,2,4 oxadiazole compounds and methods of use thereof

Publications (1)

Publication Number Publication Date
CA2725573A1 true CA2725573A1 (en) 2009-12-10

Family

ID=41398367

Family Applications (1)

Application Number Title Priority Date Filing Date
CA2725573A Abandoned CA2725573A1 (en) 2008-06-06 2008-06-06 Novel 1,2,4 oxadiazole compounds and methods of use thereof

Country Status (6)

Country Link
EP (1) EP2297140A4 (en)
JP (1) JP2011522818A (en)
CN (1) CN102089297A (en)
CA (1) CA2725573A1 (en)
MX (1) MX2010013399A (en)
WO (1) WO2009148452A1 (en)

Families Citing this family (21)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US8580842B2 (en) 2003-09-30 2013-11-12 Abbott Gmbh & Co. Kg Heteroaryl-substituted 1,3-dihydroindol-2-one derivatives and medicaments containing them
US8486979B2 (en) 2006-12-12 2013-07-16 Abbvie Inc. 1,2,4 oxadiazole compounds and methods of use thereof
US20080167286A1 (en) 2006-12-12 2008-07-10 Abbott Laboratories Pharmaceutical compositions and their methods of use
UY30846A1 (en) 2006-12-30 2008-07-31 Abbott Gmbh & Amp OXINDOL DERIVATIVES REPLACED, MEDICINES THAT UNDERSTAND AND USE THEMSELVES
ES2398676T3 (en) 2007-12-07 2013-03-20 Abbott Gmbh & Co. Kg Amidomethyl substituted oxindole derivatives and their use for the production of a drug for the treatment of vasopressin-dependent diseases
KR20100097195A (en) 2007-12-07 2010-09-02 애보트 게엠베하 운트 콤파니 카게 5-halogen-substituted oxindole derivatives and use thereof for treating vasopressine-dependent diseases
ES2455197T3 (en) 2007-12-07 2014-04-14 Abbvie Deutschland Gmbh & Co Kg 5,6-disubstituted oxindole derivatives and their use for the preparation of a medicine for the treatment of vasopressin-dependent diseases
US8703774B2 (en) 2007-12-07 2014-04-22 AbbVie Deutschland GmbH & Co. KG Carbamate-substituted oxindole derivatives and use thereof for the treatment of vasopressin-dependent diseases
EP2435080A2 (en) 2009-05-29 2012-04-04 Abbott Laboratories Pharmaceutical compositions for the treatment of pain
BR112012033402A2 (en) * 2010-07-02 2017-01-24 Gilead Sciences Inc ion channel modulators according to fused heterocyclic compounds
US9173396B2 (en) 2010-10-22 2015-11-03 Bayer Intellectual Property Gmbh Heterocyclic compounds as pesticides
EA028156B9 (en) 2011-05-10 2018-01-31 Джилид Сайэнс, Инк. Fused heterocyclic compounds as ion channel modulators
TWI549944B (en) 2011-07-01 2016-09-21 吉李德科學股份有限公司 Fused heterocyclic compounds as ion channel modulators
NO3175985T3 (en) 2011-07-01 2018-04-28
US20140329797A1 (en) * 2011-10-17 2014-11-06 Bayer Intellectual Property Gmbh Substituted oxadiazolyl pyridinones and oxadiazolyl pyridazinones as hif inhibitors
AU2013286860B2 (en) 2012-07-02 2017-10-26 Monsanto Technology Llc Processes for the preparation of 3,5-disubstituted-1,2,4-oxadiazoles
CN106316885B (en) * 2015-07-03 2019-02-12 普济生物科技（台州）有限公司 A kind of preparation method of 3- [5- (2- fluorophenyl) -1,2,4- oxadiazoles -3- base] benzoic acid
BR112019026478A2 (en) 2017-06-14 2020-07-14 Trevena, Inc. compounds for modulating s1p1 activity and methods of using them
RU2671563C1 (en) * 2017-11-13 2018-11-02 Федеральное государственное бюджетное учреждение науки Южный научный центр Российской академии наук 2-phenyl-5-(3,4,5-triethoxyphenyl)-1,3,4-oxadiazole with luminescent activity
CA3158600A1 (en) 2019-11-19 2021-05-27 Brian H. Heasley Compounds and methods of preparing compounds s1p1 modulators
CN111138376A (en) * 2019-12-24 2020-05-12 中山大学 3, 5-disubstituted phenyl-1, 2, 4-oxadiazole derivative and preparation method and application thereof

Family Cites Families (8)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4022901A (en) * 1975-03-05 1977-05-10 E. R. Squibb & Sons, Inc. 3-Pyridinyl-5-isothiocyanophenyl oxadiazoles
CH623971B5 (en) * 1976-06-04 1982-01-15 Hoechst Ag Process for the production of new benzofuran derivatives and their use as optical brighteners.
WO1993013083A1 (en) * 1991-12-31 1993-07-08 Fujisawa Pharmaceutical Co., Ltd. Oxadiazole derivatives having acetylcholinesterase-inhibitory and muscarinic agonist activity
EP1781289A1 (en) * 2004-07-22 2007-05-09 PTC Therapeutics, Inc. Thienopyridines for treating hepatitis c
EP1833804A1 (en) * 2004-12-28 2007-09-19 AstraZeneca AB Aryl sulphonamide modulators
CA2606087A1 (en) * 2005-04-26 2006-11-02 Neurosearch A/S Novel oxadiazole derivatives and their medical use
EP2255848A3 (en) * 2006-09-04 2011-04-06 NeuroSearch AS Pharmaceutical combinations of a nicotine receptor modulator and a cognitive enhancer
US20080167286A1 (en) * 2006-12-12 2008-07-10 Abbott Laboratories Pharmaceutical compositions and their methods of use

Also Published As

Publication number Publication date
JP2011522818A (en) 2011-08-04
MX2010013399A (en) 2010-12-21
WO2009148452A1 (en) 2009-12-10
EP2297140A1 (en) 2011-03-23
EP2297140A4 (en) 2012-05-02
CN102089297A (en) 2011-06-08

Similar Documents

Publication Publication Date Title
US8486979B2 (en) 1,2,4 oxadiazole compounds and methods of use thereof
US9040568B2 (en) Pharmaceutical compositions for the treatment of pain
US9186407B2 (en) Pharmaceutical compositions and their methods of use
CA2725573A1 (en) Novel 1,2,4 oxadiazole compounds and methods of use thereof
US20080255203A1 (en) Heterocyclic compounds and their methods of use
JP2012521429A (en) P2X3 receptor antagonist for the treatment of pain
AU2014284616A2 (en) Novel bicyclic bromodomain inhibitors
BR112015001502B1 (en) USE OF AZAINDAZOLE OR DIAZAINDAZOLE DERIVATIVES IN THE TREATMENT OR PREVENTION OF PAIN
WO2015004533A2 (en) Novel substituted bicyclic compounds as bromodomain inhibitors
JP2012521428A (en) P2X3 receptor antagonist for the treatment of pain
JP2017512786A (en) TrkA kinase inhibitors, compositions and methods thereof
WO2018130869A1 (en) Benzodiazepine derivatives, compositions, and methods for treating cognitive impairment
JP2016528266A (en) V1a antagonists for treating phase shift sleep disorders
WO2020065048A1 (en) Novel compounds as nadph oxidase inhibitors
TW202220990A (en) Heteroaryl substituted spiropiperidinyl derivatives and pharmaceutical uses thereof
CN101605542A (en) Pharmaceutical composition with and using method
OA19667A (en) Benzodiazepine derivatives, compositions, and methods for treating cognitive impairment.

Legal Events

Date Code Title Description
FZDE Dead

Effective date: 20140606