CA2715100C - A novel receptor trem(triggering receptor expressed on myeloid cells) and uses thereof - Google Patents

Abstract

Novel activating receptors of the Ig super-family expressed on human myeloid cells, called TREM(s)(triggering receptor expressed on myeloid cells) are provided. Specifically, two (2) members of TREMs, TREM-1 and TREM-2 are disclosed. TREM-1 is a transmembrane glycoprotein expressed selectively on blood neutrophils and a subset of monocytes but not on lymphocytes and other cell types and is upregulated by bacterial and fungal products. Use of TREM-1 in treatment and diagnosis of various inflammatory diseases are also provided. TREM-2 is also a transmembrane glycoprotein expressed selectively on peripheral deindritic cells (DCs) but not on granulocytes or monocytes. DC stimulation via TREM-2 leads to DC maturation and resistance to apoptosis, and induces strong upregulation of CCR7 and subsequent chemotaxis toward macrophage inflammatory protein 3- .beta. TREM-2 has utility in modulating host immune responses in various immune disorders, including autoimmune diseases and allergic disorders

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A NOVEL RECEPTOR TREM (TRIGGERING RECEPTOR
EXPRESSED ON MYELOID CELLS) AND USES THEREOF
I. INTRODUCTION
This invention relates generally to new activating receptors of the Ig super-family expressed on human myeloid cells, called TREM (triggering receptor expressed on myeloid cells) which are involved in inflammatory responses. Specifically, this invention relates to two (2) members of TREMs, TREM-1 and TREM-2.

2. BACKGROUND OF THE INVENTION
Inflammatory responses to bacterial and fungal infections are primarily mediated by neutrophils and monocytes (Medzhitov, R. & Janeway, C., Jr., 2000, Innate immunity. N. Engl. .1. Med. 343:338-44; Hoffmann, J. A., Kafatos, F. C., Janeway, C. A. &
Ezekowitz, R. A., 1999, Phylogenetic perspectives in innate immunity. Science 284:1313-] 5 8). These cells express pattern recognition receptors (PRR) which recognize conserved molecular structures shared by groups of microorganisms (Aderem, A. &
Ulevitch, R. J., 2000, Toll-like receptors in the induction of the innate immune response.
Nature 406:782-7;
Beutler, B., 2000, Endotoxin, toll-like receptor 4, and the afferent limb of innate immunity.
Curr. Opin. Microbial. 3:23-8). Engagement of PRRs by microbial products activate signaling pathways which control the expression of a variety of genes. These inducible genes encode proinflammatory chemokines and cytokines and their receptors, as well as adhesion molecules and enzymes that produce low molecular weight proinflammatory mediators and reactive oxygen species. The combined action of all these products presumably leads to elimination of the infectious agents and tissue repair.
However, excessive secretion of pro-inflammatory mediators, together with overexpression of their receptors, cause excessive autocrine/paracrine activation of neutrophils and monocytes, leading to tissue damage and septic shock (Bone, R. C., 1991, The pathogenesis of sepsis.
Ann. Intern. Med. 115:457-69; Beutler, B., Milsark, I. W. & Cerami, A. C., 1985, Passive immunization against cachectin/tumor necrosis factor protects mice from lethal effect of endotoxin. Science 229:869-71; Morrison, D. C. & Ryan, J. L., 1987, Endotoxins and disease mechanisms. Annu. Rev. Med. 38:417-32; Tracey, K. J. et al., 1986, Shock and tissue injury induced by recombinant human cachectin. Science 234:470-4;
Glauser, M. P., Zanetti, G., Baumgartner, J. D. & Cohen, J., 1991, Septic shock: pathogenesis.
Lancet 338:732-6). Thus, the regulation of neutrophil and monocyte activation by stimulatory receptors and their ligands is crucial to the outcome of host inflammatory responses to infections.

Neutrophil- and monocyte/macrophage-mediated inflammatory responses can be stimulated through many receptors with different structures and specificities =
(Rosenberg, H.F., and 1.1. Gallin, 1999, Inflammation. In Fundamental Immunology, 4th Ed. W. E Paul, ed. Lippincott-Raven, Philadelphia p. 1051). These include G
protein-linked seven-transmembrane domain receptors specific for either fMLP, lipid mediators, complement factors, or chemokines, the Fc and complement receptors, the CD14 and Toll-like receptors for LPS, as well as the cytokine receptors for IFN-y and TNF-a (Ulevitch, RJ., and P.S. Tobias, 1999, Recognition of Gram-negative bacteria and endotoxin by the innate immune system. Curr. Opin. Immunol. 11:19). In addition, engagement of these receptors can up-regulate or "prime" the responsiveness of myeloid cells to other stimuli, potentiating the inflammatory response (Downey, G.P., T. Fukushima, L.
Fialkow, and T.K.
Waddell, 1995, Intracellular signaling in neutrophil priming and activation.
Semin. Cell Biol. 6:345).
Neutrophils and macrophages express additional activating receptors, but their role in inflammation is unknown. These receptors belong either to the Ig superfamily (Ig-SF), such as Ig-like transcripts (ILT)/leukocyte Ig-like receptors (LIR)/monocyte/macrophage Ig-like receptors (MIRs), paired Ig-like receptor (PIR-As), and signal regulatory protein PI (SIRP131), or to the C-type lectin superfamily, such as myeloid DAP12-associating lectin-I (MDL-1) (Nakajima, H., J. Samaridis, L. Angman, and M.
Colonna, 1999, Human myeloid cells express an activating ILT receptor (ILT 1) that associates with Fc receptor y-chain. J. Immunol. 162:5; Yamashita, Y., M. Ono, and T.
Takai, 1998, Inhibitory and stimulatory functions of paired Ig-like receptor (PIR) family in = RBL-2H3 cells. .1. Immunol. 161:4042; Kubagawa, H., C.C. Chen, L.H. Ho, T.S. Shimada, L. Gartland, C. Mashburn, T. Uehara, J.V. Ravetch, and M.D. Cooper, 1999, Biochemical nature and cellular distribution of the paired immunoglobulin-like receptors, PIR-A and P1R-B. J. Exp. Med. 189:309; Dietrich, J., M. Cella, M. Seiffert, H.-J.
Biihring, and M.
Colonna, 2000, Signal-regulatory protein 01 is a DAP12-associated activating receptor expressed in myeloid cells. J. Immunol_ 164:9; Bakker, A.B., E. Baker, G.R.
Sutherland, J.H. Phillips, and L.L. Lanier, 1999, Myeloid DAP12-associating lectin (MDL)-1 is a cell surface receptor involved in the activation of myeloid cells. Proc. Natl.
Acad. Sci. USA
96:9792). Typically, all of these receptors bear some homology with activating NK cell receptors (Lanier, L. L., 1998, NK cell receptors. Al11711. Rev. Iminunol.
16:359). In particular, they contain a short intracellular domain that lacks docking motifs for signaling mediators and a transmcmbrane domain with a positively charged amino acid residue. This residue allows pairing with transmembrane adapter proteins, which contain a negatively charged amino acid in the transmembranc domain and a cytoplasmic immunoreceptor -j,-tyrosine-based activation motif (ITAM). Specifically, ILT/LIR/MIR and Pills-are coupled with the y-chain of the Fc receptors (FcRy) (Nakajima, H., supra; Yamashita, Y., supra;
Kubagawa, H., supra), whereas SIRPf31 and MDL-1 pair with DAP12 (Dietrich, J., supra;
Bakker, A.B., supra). Upon ITAM phosphorylation, these adapters recruit protein tyrosine kinases, which initiate a cascade of phosphorylation events that ultimately lead to cell activation.
DAP12-deficient mice exhibit a dramatic accumulation of dendritic cells (DCs) in muco-cutaneous epithelia, associated with an impaired hapten-specific contact sensitivity (Bakker, A. B., Hoek, R. M., Cerwenka A., Blom, B., Lucian, L., McNeil, T., Murray, R., Phillips, L.H., Sedgwick, J. D., and Lanier L. L., 2000, DAP12-deficient mice fail to develop autoimmunity due to impaired antigen priming. Immunity 13:345-53;
Tomasello, E., Desmoulins, P. 0., Chemin, K., Guia, S., Cremer, H., Ortaldo, J., Love, P., Kaiserlian, D., and Vivier, E., 2000, Combined natural killer cell and dendritic cell = functional deficiency in KASRAP/DAP12 loss-of-function mutant mice.
Immunity 13:355-64). Furthermore, recent evidence suggests that the interaction between CCR7 (CC family chemokine receptor no. 7) and ELC (Epstein-Barr virus-induced molecule 1 ligand chemokine) triggers DC trafficking to the lymph nodes. In particular, skin DCs from CCR7 -/- mice, as well as in DAP12 -/- mice, are severely impaired in migrating to the draining LNs following activation (Foster, R., Schubel, A., Breitfeld, D., Kremmer, E., Renner-Muller, I., Wolf, E., and Lipp, M., 1999, CCR7 coordinates the primary immune response by establishing functional microenvironments in secondary lymphoid organs.
Cell 99:23-33). However, the DAP12-associated receptor responsible for these phenotypes is yet unknown.
The recent discovery of a new DAP12-associated receptor on NK cells, called NKp44 (Cantoni, C., C. Bottino, M. Vitale, A. Pessino, R. Augugliaro, A. Malaspina, S. Parolini, L. Moretta, A. Moretta, and R. Biassoni, 1999, NKp44, a triggering receptor involved in tumor cell lysis by activated human natural killer cells, is a novel member of the immunoglobulin superfamily. J. Exp. Med. 189:787), suggested the possible existence of yet unknown DAP12- associated receptors also on other cells involved in innate responses.
The present inventors have identified new immunoglobulin-super-family (Ig-SF) receptors designated as TREMs (triggering receptor expressed on myeloid cells), that are involved in the regulation of a variety of cellular responses, especially inflammatory responses as well as trafficking of DCs.
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- 3 -3. SUMMARY OF INVENTION -The present invention is based upon the inventors' identification of two cDNA molecules which encode triggering receptors expressed on myeloid cells (TREM-1:
SEQ ID NO:1; and TREM-2: SEQ ID NO:2). These molecules are expressed on human myeloid cells and are novel transmembrane proteins of the immunoglobulin superfamily (1g-SF).
TREM-1 is a transmembrane glycoprotein having the amino acid sequence of SEQ ID NO:3 which is selectively expressed on blood neutrophils and a subset of monocytes but not on lymphocytes and other cell types and is up-regulated by bacterial LPS. TREM-1 has utility in the regulation of acute inflammations.
The TREM-2 is a transmembrane glycoprotein having the amino acid sequence of SEQ ID NO:4 which is selectively expressed on dendritic cells (DCs) but not on granulocytes or monocytes. Stimulation of DCs via TREM-2 leads to maturation of DCs, renders them resistant against apoptosis, and induces strong upregulation of CCR7 and subsequent chemotaxis towards ELC/MIP3-f3 (macrophage inflammatory protein 343).
TREM-2 has utility in the regulation of dendritic cell function.
Thus, the members of TREM (TREM or TR_EMs) may be useful in regulating a variety of cellular processes, especially inflammatory responses and dendritic cell functions, therefore have a great potential for therapeutic as well as diagnostic uses.
Accordingly, this invention provides isolated or recombinantly prepared TREMs, or fragments, homologues, derivatives, or variants thereof, as defined herein, which are herein collectively referred to as "peptides of the invention" or "proteins of the invention." Furthermore, this invention provides nucleic acid molecules encoding the polypeptide of the invention, which are herein collectively referred to as "nucleic acids of the invention" and include cDNA, genomic DNA, and RNA.
Accordingly, this invention provides isolated nucleic acid molecules which comprise or consist of a nucleotide sequence that is about 30%, 35%, 40%, 45%, 50%, 55%, 60%, 65%, 70%, 75%, 80%, 85%, 90%, 95%, or 98% identical to the nucleotide sequence of SEQ ID NO:1, 2, or a complement thereof. In specific embodiments, such nucleic acid molecules exclude nucleotide sequences of accession nos_ D78812, A133 7247, AW 139572, AW274906, AW139573, A1394041, A1621023, A1186456, A1968134, A1394092, A1681036, A1962750, AA494171, AA099288, AW139363, AW135801, AA101983, and N41388.
This invention further provides isolated nucleic acid molecules which comprise or consist of about 25, 30, 35, 40, 45, 100, 150, 200, 250, 300, 350, 400, 450, 500, 550, 600, 650, 700, 750, 800, 850, or more contiguous nucleotides of the nucleotide

- 4 -sequence of SEQ ID NO:1, or a complement thereof. In specific embodiments", such nucleic acid molecules exclude nucleotide sequences of accession nos. D788I2, AI337247, AW139572, AW274906, AW139573, A1394041, A1621023, A1186456, A1968134, A1394092, A1681036, A1962750, AA494171, AA099288, AW139363, AW135801, and AA101983.
This invention further provides isolated nucleic acid molecules which comprise or consist of about 25, 30, 35, 40, 45, 100, 150, 200, 250, 300, 350, 400, 450, 500, 550, 600, 650, 700, 750, 800, 850, 900, 950, 1000, or more contiguous nucleotides of the nucleotide sequence of SEQ ID NO:2, or a complement thereof. In specific embodiments, such nucleic acid molecules exclude the nucleotide sequence of accession no.
N41388.
The invention provides isolated polypeptides or proteins which are encoded .by a nucleic acid molecule consisting of or comprising a nucleotide sequence that is at least about 30%, 35%, 40%, 45%, 50%, 55%, 60%, 65%, 70%, 75%, 80%, 85%, 90%, 95%, or 98% identical to the nucleotide sequence of SEQ ID NO:1 or a complement thereof, or SEQ
ID NO:2 or a complement thereof. In specific embodiments, such polypeptides or proteins exclude polypeptides or proteins encoded by nucleotide sequences of accession nos.
D78812, A1337247, AW139572, AW274906, AW139573, A1394041, AI621023, A1186456, A1968134, A1394092, A1681036, A1962750, AA494171, AA099288, AW139363, AW135801, AA101983, and N41388.
The invention provides isolated polypeptides or proteins which are encoded by a nucleic acid molecule consisting of or comprising a nucleotide sequence that is at least about 25, 30, 35, 40, 45, 100, 150, 200, 250, 300, 350, 400, 450, 500, 550, 600, 650, 700, 750, 800, 850, or more contiguous nucleotides of the nucleotide sequence of SEQ ID NO:1 or a complement thereof. In specific embodiments, such polypeptides or proteins exclude polypeptides or proteins encoded by nucleotide sequences of accession nos.
D78812, A1337247, AW139572, AW274906, AW139573, A1394041, A1621023, A1186456, AI968134, A1394092, AI681036, A1962750, AA494171, AA099288, AW139363, AW135801, and AA101983.
The invention provides isolated polypeptides or proteins which are encoded by a nucleic acid molecule comprising a nucleotide sequence that is at least about 25, 30, 35, 40, 45, 100, 150, 200, 250, 300, 350, 400, 450, 500, 550, 600, 650, 700, 750, 800, 850, 900, 950, 1000 or more contiguous nucleotides of the nucleotide sequence of SEQ ID NO:2 or a complement thereof. In specific embodiments, such polypeptides or proteins exclude a polypeptide encoded by the nucleotide sequence of accession no. N41388.
The invention provides isolated nucleic acid molecules comprising a nucleotide sequence encoding a protein having an amino acid sequence that is at least about

-5-.

40%, 45%, 50%, 55%, 60%, 65%, 70%, 75%, 80%, 85%, 90%, 95%, or 98% identical to the amino acid sequence of SEQ ID NO:3 or 4, or fragments, homologues, derivatives, or variants of said protein, or complement of said nucleic acid molecules. In specific embodiments, such nucleic acid molecules exclude nucleotide sequences of accession nos.
D78812, A1337247, AW139572, AW274906, AW139573, A1394041, A1621023, A1186456, A1968134, A1394092, A1681036, A1962750, AA494171, AA099288, AW139363, AW135801, AA101983, and N41388.
The invention provides isolated nucleic acid molecules comprising a nucleotide sequence encoding a protein having an amino acid sequence that comprises or consists of at least about 10, 15, 20, 25, 30, 50, 75, 100, 125, 150, 175, 200, 225, 230 or more contiguous amino acids of SEQ ID NO:3, or fragments, homologues, derivatives, or variants of said protein, or complements of said nucleic acid molecules. In specific embodiments, such nucleic acid molecules exclude nucleotide sequences of accession nos.
= D78812, A1337247, AW139572, AW274906, AW139573, A1394041, A1621023, All 86456, A1968134, A1394092, A1681036, A1962750, AA494171, AA099288, AW139363, AW135801, and AA101983.
The invention provides isolated nucleic acid molecules comprising a nucleotide sequence encoding a protein having an amino acid sequence that comprises or consists of at least about 10, 15, 20, 25, 30, 50, 75, 100, 125, 150, 175, 200, 225, or more contiguous amino acids of SEQ ID NO:4, or fragments, homologues, derivatives, or variants of said protein, or complements of said nucleic acid molecules. In specific embodiments, such nucleic acid molecules exclude the nucleotide sequence of accession no.
N41388.
Furthermore, the invention provides isolated polypeptides or proteins comprising an amino acid sequence that is at least about 40%, 45%, 50%, 55%, 60%, 65%, 70%, 75%, 80%, 85%, 90%, 95%, or 98% identical to the amino acid sequence of SEQ ID
NO:3 or 4, or fragments, homologues, derivatives, or variants thereof. In specific embodiments, such polypeptides or proteins exclude polypeptides or proteins encoded by nucleotide sequences of accession nos. D78812, AI337247, AW139572, AW274906, AW139573, A1394041, A1621023, A1186456, A1968134, A1394092, A1681036, A1962750, AA494171, AA099288, AW139363, AW135801, AA101983, and N41388.
The invention provides isolated polypeptides or proteins comprising an amino acid sequence that comprises or consists of at least about 10, 15, 20, 25, 30, 50, 75, 100, 125, 150, 175, 200, 225, 230 or more contiguous amino acids of SEQ ID
NO:3, or fragments, homologues, derivatives, or variants thereof. In specific embodiments, such polypeptides or proteins exclude polypeptides or proteins encoded by nucleotide sequences

- 6 -of accession nos. D78812, AI337247, AW139572, AW274906, AW139573, A1394041, A1621023, A1186456, A1968134, A1394092, A1681036, A1962750, AA494171, AA099288, AW139363, AW135801, and AA101983.
The invention provides isolated polypeptides or proteins comprising an amino acid sequence that comprises or consists of at least about 10, 15, 20, 25, 30, 50, 75, 100, 125, 150, 175, 200, 225, or more contiguous amino acids of SEQ ID NO:4, or fragments, homologues, derivatives, or variants thereof. In specific embodiments, such polypeptides or proteins exclude a polypeptide encoded by the nucleotide sequence of accession no. N41388.
In preferred embodiments, such fragments, homologues, derivatives or variants of TREM-1 or TREM-2 have a biological activity of a TREM-1 or TREM-2 full-length protein, such as antigenicity, immunogenicity, triggering of proinflammatory chemokines and cytokines, mobilization of cytosolic Ca2', protein tyrosine-phosphorylation, and other activities readily assayable.
In one embodiment, this invention provides isolated nucleic acid molecules which hybridize under stringent or moderately stringent conditions, as defined herein, to a nucleic acid having the sequence of SEQ ID NO:! or 2, or a complement thereof.

Furthermore, this invention also provides nucleic acid molecules which are suitable for use as primers or hybridization probes for the detection of nucleic acids 70 encoding a polypeptide of the invention.
In one embodiment, the invention provides an isolated nucleic acid molecule which is antisense to the coding strand of a nucleic acid of the invention.
Another aspect of the invention provides vectors, e.g., recombinant expression vectors, comprising a nucleic acid molecule of the invention.
Further, the invention also provides host cells containing such a vector or engineered to contain and/or express a nucleic acid molecule of the invention and host cells containing a nucleotide sequence of the invention operably linked to a heterologous promoter. The invention also provides methods for preparing a polypeptide of the invention by a recombinant DNA
technology in which the host cells containing a recombinant expression vector encoding a polypeptide of the invention or a nucleotide sequence encoding a polypeptide of the invention operably linked to a heterologous promoter, are cultured, and the polypeptide of the invention produced and isolated.
The invention further provides antibodies that specifically bind a polypeptide of the invention. Such antibodies include, but are not limited to, polyclonal, monoclonal, hi-specific, multi-specific, human, humanized, chimeric antibodies, single chain antibodies, Fab fragments, F(ab)2 fragments, disulfide-linked Fvs, and fragments containing either a

- 7 -VL or VH domain or even a complementary determining region (CDR) that specifically binds to a polypeptide of the invention.
In one embodiment, the invention provides methods for detecting the presence, activity or expression of a polypeptide of the invention in a biological material, such as cells, blood, saliva, urine, and so forth. The increased or decreased activity or expression of the polypeptide in a sample relative to a control sample can be determined by contacting the biological material with an agent which can detect directly or indirectly the presence, activity or expression of the polypeptide of the invention.
In another embodiment, an agent modulates the expression of a polypeptide of the invention by modulating transcription, splicing, or translation of an mRNA encoding a polypeptide of the invention. In one embodiment, such an agent is a nucleic acid molecule having a nucleotide sequence that is antisense to all or a portion of the coding strand of an mRNA encoding a polypeptide of the invention.
The invention also provides methods for modulating the activity of a polypeptide of the invention comprising contacting a cell with an agent that modulates (e.g., inhibits or stimulates) the activity or expression of a polypeptide of the invention such that activity or expression in the cell is modulated. In one embodiment, such a modulating agent is an antibody that is specific for a polypeptide of the invention. In another embodiment, the agent is a fragment of a polypeptide of the invention or a nucleic acid molecule encoding such a polypeptide fragment.
In another aspect, the present invention provides methods for identifying a compound or ligand that binds to or modulates the activity of a polypeptide of the invention. Such a method comprises measuring a biological activity of the polypeptide in the presence or absence of a test compound and identifying test compounds that alter (increase or decrease) the biological activity of the polypeptide. In another aspect, the invention provides a method for identifying a compound that modulates the expression of a polypeptide or nucleic acid of the invention by measuring the expression of the polypeptide or nucleic acid in the presence or absence of the compound.
In one embodiment, the invention provides a fusion protein comprising a bioactive molecule and one or more domains of a polypeptide of the invention or fragment thereof. In particular, the present invention provides fusion proteins comprising a bioactive molecule recombinantly fused or chemically conjugated (including both covalent and non-covalent conjugations) to one or more domains of a polypeptide of the invention or fragments thereof.
The present invention also provides methods for treating a subject having a disorder which is characterized by aberrant activity of a polypeptide of the invention or

- 8 -aberrant expression of a nucleic acid of the invention by administering an agdni which is a modulator of the activity of a polypeptide of the invention or a modulator of the expression of a nucleic acid of the invention to the subject. In one embodiment, such modulator is a polypeptide of the present invention or fragments thereof. In another embodiment, such modulator is a nucleic acid of the invention (e.g., gene therapy). In another embodiment, the modulator may be an antibody which is specific to a polypeptide of the invention.
Furthermore, the invention provides a pharmaceutical composition comprising a polypeptide or nucleic acid molecule of the present invention or an antibody or fragments thereof specific to a polypeptide of the invention.
The invention further provides a kit containing a polypeptide, nucleic acid molecule of the present invention or an antibody or fragments thereof specific to a polypeptide of the invention.
3.1 Definitions The term "immunoglobulin superfamily" or "Ig-SF" refers to a group of cell membrane proteins having a common structure similar to an immunoglobulin constant region (CI-type and C2-type) or variable region (V-type). The prototype of V-type domains are the variable domains of immunoglobulins and T-cell receptors. V-type immunoglobulin domains are larger than Cl and C2 domains. Some proteins carry many such domains and others few.
The term "triggering receptor expressed on myeloid cells" or "TREM" refers to a group of activating receptors which are selectively expressed on different types of myeloid cells, such as monocytes, macrophages, dendritic cells (DCs), and neutrophils, and may have a predominant role in inflammatory responses. TREMs are primarily transmembrane glycoproteins with a Ig-type fold in their extracellular domain and, hence, belong to the Ig-SF. These receptors contain a short intracellular domain, but lack docking motifs for signaling mediators and require adapter proteins, such as DAP12, for cell activation.
The term "myeloid cells" as used herein refers to a series of bone marrow-derived cell lineages including granulocytes (neutrophils, eosinophils, and basophils), monocytes, macrophages, and mast cells. Furthermore, Peripheral blood dendritic cells of myeloid origin, and dendritic cells and macrophages derived in vitro from monocytes in the presence of appropriate culture conditions, are also included.
The term "homologue," especially "TREM homologue" as used herein refers to any member of a series of peptides or nucleic acid molecules having a common biological activity, including antigenicity/immunogenicity and inflammation regulatory

- 9 -activity, and/or structural domain and, having sufficient amino acid or nucleotide sequence identity as defined herein. TREM homologues can be from either the same or different species of animals.
The term "variant" as used herein refers either to a naturally occurring allelic variation of a given peptide or a recombinantly prepared variation of a given peptide or protein in which one or more amino acid residues have been modified by amino acid substitution, addition, or deletion.
The term "derivative" as used herein refers to a variation of given peptide or protein that are otherwise modified, i.e., by covalent attachment of any type of molecule, Preferably having bioactivity, to the peptide or protein, including non-naturally occurring amino acids.
An "isolated" or "purified" peptide or protein is substantially free of cellular material or other contaminating proteins from the cell or tissue source from which the protein is derived, or substantially free of chemical precursors or other chemicals when =
chemically synthesized. The language "substantially free of cellular material"
includes preparations of a polypeptide/protein in which the polypeptide/protein is separated from cellular components of the cells from which it is isolated or recornbinantly produced. Thus, a polypeptide/protein that is substantially free of cellular material includes preparations of the polypeptide/protein having less than about 30%, 20%, 10%, 5%, 2_5%, or 1%, (by dry weight) of contaminating protein. When the polypeptide/protein is recombinantly produced, it is also preferably substantially free of culture medium, i.e., culture medium represents less than about 20%, 10%, or 5% of the volume of the protein preparation. When polypeptide/protein is produced by chemical synthesis, it is preferably substantially free of chemical precursors or other chemicals, i.e., it is separated from chemical precursors or other chemicals which are involved in the synthesis of the protein.
Accordingly, such preparations of the polypeptide/protein have less than about 30%, 20%, 10%, 5%
(by dry weight) of chemical precursors or compounds other than polypeptide/protein fragment of interest. In a preferred embodiment of the present invention, polypeptides/proteins are isolated or purified.
An "isolated" nucleic acid molecule is one which is separated from other nucleic acid molecules which are present in the natural source of the nucleic acid molecule.
Moreover, an "isolated" nucleic acid molecule, such as a cDNA molecule, can be substantially free of other cellular material, or culture medium when produced by recombinant techniques, or substantially free of chemical precursors or other chemicals when chemically synthesized. In a preferred embodiment of the invention, nucleic acid molecules encoding polypeptides/proteins of the invention are isolated or purified.
- JO-Other abbreviations used herein are: SIRPf11, signal regulatory protein f31;
HA, hemagglutinin; TN?, 2,4,6-trinitrophenyl; MCP, monocyte chemoattractant protein;
PLC-y, phospholipase C-7; DC, dendritic cell; MPO, myeloperoxidase; 1TAM, immunoreceptor tyrosine-based activation motif; ERK, extracellular signal-related kinase;
mAb, monoclonal antibody.
The names of amino acids referred to herein are abbreviated either with three-letter or one-letter symbols.
4. DESCRIPTION OF THE FIGURES
Figure I. shows the predicted amino acid sequences of TREM-1 (SEQ ID NO:3)and TREM-2 (SEQ ID NO:4), respectively. The Signal peptide is indicated in lower-case letters. The potential N-glycosylation sites are indicated by asterisks. The cysteines potentially involved in generating the intrachain disulfide bridge of the Ig-SF V-type fold are marked in bold and are shown in the context of their flanking consensus sequences (boxed). The predicted transmembrane domain is underlined, and the charged lysine residue is also marked in bold and boxed.
Figure 2 shows the mRNA sequence of TREM-I.
Figure 3 shows the mRNA sequence of TREM-2.
Figures 4(a)-(c) show the result of FACS analysis for cell surface expression 90 of transfected cDNAs, i.e., TREM-1 FLAG (a), TREM-1 1 z LAF G/DAp= ^11A
(b), or NKp44FLAG/DAP12" (c), in COS-7 cells. Cells were analyzed by FACS with mAb (anti-TREM-I, IgG1). The percentage of TREM-1 positive cells (upper right quadrant) is indicated. Expression of TREM-l", NKp44FLAc, and DApi2mA was confirmed using anti-FLAG and anti-HA mAbs (data not shown). Cells stained with a control Ab were contained within the lower right quadrant.
Figures 5(a)-(g) show the result of three-color FACS analysis of whole blood leukocytes using mAbs 21C7 (anti-TREM-1, IgGl; (a)), 3C10 (anti-CD14, IgG2b;
(b)), and L243 (anti-HLA-DR, IgG2a) followed by isotype-specific FITC/PE/biotin-conjugated secondary Abs and further APC-labeled streptavidin. High side scatter cells correspond to TREM-14 neutrophils (c). Low side scatter cells include CDIesh/HLA-DR4 cells (monocytes; (d)), CD14d1m/HLA-DR4 (monocytes; (e)), CD14"/HLA-DR4 cells (which =
include B cells and DCs; (0), and CD14"/HLA-DR- cells (mostly lymphocytes;
(g)).
Figures 6(a)-(b) show the result of three-color FACS analysis of monocytes (a) and neutrophils (b) which were stimulated with LPS (1 gg/ml) for 16 h and stained with either rnAb 21C7 or mAb 1B7.11, which is a control IgG1 (anti-2,4,6-trinitrophenyl (TNP)), followed by human immunoglobulin-adsorbed PE-conjugated goat anti-mouse lgG.
- II -LPS-treated monocytes or neutrophils are expressed with a solid bold line, whereas LPS-untreated cells are expressed with a solid line. The background staining with a control IgG1 mAb is shown as a dashed line.
Figures 7(A)-(H) show the TREM-I -mediated cytokine production and degranulation by neutrophils and monocytes that are reacted with mAb 21C7 or 1F11 (anti-MHC class I) in the presence or absence of LPS (1 jig/m1). Secretion of IL-8 (A) and MPO
(B) by neutrophils and that of MCP-1 (D), IL-8 (E), and TNF-a (F) by monocytes was measured by ELISA and the results are shown in the upper panels. TREM-1-mediated degranulation of neutrophils (C) and secretion of TNF-a (G) and MCP-1 (H) by monocytes after priming these cells with LPS are shown in the lower panels.
Figures 8(a)-(c) show the result of intracellular calcium measurements in monocytes treated with anti-TREM-1 alone (b) or in combination with a cross-linking Ab (c). Intracellular calcium was also measured for monocytes which were treated with a control IgG1 mAb (anti-MHC class I) and a cross-linking Ab (a). Addition of Abs is indicated by an arrow.
Figure 9 shows the anti-phosphotyrosine blot of cell lysates from monocytes stimulated with anti-TREM-1 or control IgG1 mAbs in the presence of a cross-linking Ab for the indicated time periods.
Figures 10(a) and (b) show the result of Western blot in which the lysates of monocytes stimulated with anti-TREM-1 or a control antibody (anti-MHC class I
mAb) were immunoblotted with anti-phospho-ERK1/2 (a) and anti-ERK1/2 (b) mAbs.
Phosphorylated proteins are indicated by arrows in all panels. Molecular weight markers are also shown.
Figures 10(c) and 10(d) show the result of Western blot in which tyrosine phosphorylated proteins were precipitated from the lysate of monocytes stimulated with anti-TREM-1 or a control antibody and immunoblotted with anti-phospholipase C-y (PLC-y) (c)or anti-Hck (d) Abs. Anti-Hck blotting was performed as a loading control because phosphorylation of Hck is similar in both stimulated and unstimulated monocytes.
Phosphorylated proteins are indicated by arrows in all panels. Molecular weight markers are also shown.
Figure 11 shows the result of Western blot analysis under reducing condition in which the surface-biotinylated monocyte lysates were immunoprecipitated with anti-TREM-1 mAb or a control IgG1 (anti-MHC class I mAb) and left untreated or treated with N-glycanase F followed by Streptavidin-HRP blot. Deglycosylated TREM-1 is indicated as TREM-1D'g'''.

Figure 12 shows the result of Western blot analysis in which Fietvanadate-treated monocytes were subjected to immunoprecipitation with anti-TREM-1 mAb, anti-SIRP mAb as a positive control, or control IgGI (anti-MI-IC class I mAb). The precipitates were analyzed by anti-phosphotyrosine blot under reducing and nonreducing conditions.
Figure 13 shows the result of anti-DAP12 blot analysis of a TREM-1 immunoprecipitate from monocytes (reducing conditions). Control IgG1 (anti-MHC
class I
mAb) and anti-SIRP mAb immunoprecipitates were included as negative and positive control, respectively. TREM-1 and DAP12 are indicated by arrows. Molecular weight markers are also shown.
Figures 14(A)-(B) show the result of three-color FACS analysis for TREM-1 expression on monocytes and neutrophils which were stimulated with heat-inactivated gram-negative (Pseudomonas aeruginosa; A(1) and A(2)) or gram-positive (Staphylococcus aureus; A(3) and A(4)) bacteria, or mycobacteria (Bacillus of Calrnette-Guerin; A(5) and A(6)) as well as with their cell wall components Lipopolysaccharide (100 ng/ml; B(1) and is B(2)), Lipoteichoic acid (100 ng/ml; B(3) and B(4)), Mycolic acid (10 p.g/m1; B(5) and B(6)).
Figures 15(a)-(d) show the result of three-color FACS analysis of monocytes which were stimulated with proinflammatory cytokines such as TNF-a (20 ng/ml;
(a)), IL-113 (20 ng/ml; (b)), TGFI3 (20 ng/ml; (c)), and IL-10 (20 ng/ml; (d)).
Figures 16(a)-(b) show the effect of TREM-1 ligation on LPS-mediated release of TNF-a (Fig. 16(a)) and IL-113 (Fig. 16(b)) by monocytes which were measured by ELISA. All data points correspond to the mean standard deviation of four independent experiments.
Figures 17(a)-(h) show the result of immunohistochemical staining of acute inflammatory lesions caused by bacteria and fungi, using anti-TREM-1 mAb (Figs. (a), (c), (e) and (g)) and control IgGoc mAb (Figs. (b), (d), (f) and (h)). Figs. 17(a)-(b): Acute cutaneous folliculitis caused by Staphylococcus aureus; Figs. 17(c)-(d):
Impetigo caused by Staphylococcus aureus; Fig. 17(e)-(f): Cat scratch granuloma induced by Bartonella henselae; Fig. l 7(g)-(h): Granuloma caused by Aspergillus fUmigatus.
Figures 18(a)-(f) show the result of immunohistochemical staining of tissues with non-pathogenic inflammations. Figs. 18(a) and 18(b): Psoriasis; Figs.
18(c) and 18(d):
Ulcerative colitis; and Figs. 18(e) and 18(f): Vasculitis caused by immune complexes. Figs.
18(a), (c) and (e) are stained by anti-CD IS mAb (staining granulocytes), whereas Figs.
18(b), (d), and (f) are stained by anti-TREM-1 mAb.
Figures 19(a)-(d) show the results of flow cytometric analysis of peritoneal lavagc cells from patients with aseptic SIRS due to aseptic cholecystitis (a) or polymicrobial gram-positive sepsis caused by bowel perforation (b). CD15h ' cells correspond to neutrophils. Four-colour analysis of peritoneal leucocytes from LPS-treated C57BL/6 mice (d) compared to control animals (c). Ly-6Ghigh /TREM-1 hi cells correspond to murine neutrophils. The Ly-6GI0-""d6"/TREM-1 high cells are CD1 1 b/Mac-l'(data not shown) and therefore correspond to peritoneal macrophages. Staining with isotype-matched control mAbs were set to the indicated lower quadrants.
Figure 20 shows the comparison of prophylactic effects between huTREM-1 and mTREM-1 in mice with LPS-induced septic shock. Mice (5 mice per group) were treated with either human IgG1 oc (500 p.g/mouse, i.p.; open circles), purified human or mouse TREM-1-IgGI (500 g/mouse, i.p.; closed and open squares, respectively).
One hour later, all mice received an LD100 of LPS (20 mg/kg, i.p.). One of four (4) representative experiments is shown.
Figure 21(a) shows the survival curve of C57BL/6 mice treated with control huIgG1 (closed circles) or mTREM-1-IgG I (open circles) 1 hour prior to administration of is LPS. Data points are from seven independent experiments, each of which included 5-10 animals per group. Survival was 76% (37 of 49) in mice treated with mTREM-1-IgG1 and 6% (3 of 49) in mice treated with huIgG I (P = 0.0002, two-tailed Fisher's exact test). In additional controls, mice received injections with purified human ILT3-IgG1 (closed squares, n 25) or heat-inactivated mTREM-1-IgG I (closed triangles; n = 10) before induction of endotoxemia.
Figure 21(b) shows the estimation of the LPS LDso in mice treated with mTREM-1-1gG1 or huIgGl. Mice were randomly assigned to 20 groups each containing 10 animals. Ten groups received intraperitoneal injections of mTREM-1-IgGl, whereas 10 groups were injected with hulgGl. One hour later, endotoxemia was induced by application of various quantities of LPS as indicated. Calculation of LDso was accomplished as previously described (LD5 omTRLA1- 1-IgG I = 621 jig, LD50hulscd = 467 jig; P
<0.0001) (Beutler, B., et al., 1985, Passive immunization against cachectin/tumor necrosis factor protects mice from lethal effect of endotoxin. Science 229:869-71).
Figure 21(c) shows the survival curve of mice with LPS-induced lethal 3() peritonitis. Mice were injected with LPS one (white circles), two (light grey circles), four (dark grey circles) and six hours (black circles) prior to administration of mTREM-1-1gG1 Data points are from two independent experiments, which included 3-7 animals per group.
Survival was 80% (P = 0.0007, two-tailed Fisher's exact test), 60% (P =
0.0108, two-tailed Fisher's exact test), 40% and 0%, respectively.
Figures 22(a) and (b) show the scrum levels of TNF-a (a) and IL-l3 (b) during LPS-induced septic shock. Female, 6-8-week old C57BL/6 mice (6 mice per group) treated with either human IgG1,ic (500 g/mouse, i.p.; closed squares) or mTREM-1-IgG I
(500 j.i.g/mouse, i.p.; closed diamonds) prior to injection with an LD100 of LPS (20 mg/kg, i.p.). Serum levels of TNF-a and IL- 113 at 1, 2,4, 6, 8, and 24 hours after LPS
administration, were determined by ELISA.
Figures 22(c) and (d) show the analysis of peritoneal lavage cells during LPS-induced septic shock. Mice were treated, as described for Fig. 19(a), with human IgGloc (500 fig/mouse, i.p.; open circle) or mTREM-1-IgG1 (500 ps/mouse, i.p.;
closed circle), and peritoneal lavage cells were collected at 2, 4, 6, 8, and 24 hours after LPS
administration. Neutrophils (c) and macrophages (d) were quantified on cytospin slides.
Figure 23(a) shows the survival curve of C57BL/6 mice that were injected intraperitoneally with mTREM-1-IgG1 (open circles) or hulgG1 (closed circles) one hour before intraperitoneal administration of E. coil. Data points are from two independent experiments, which included 5-15 animals per group. Survival was 55% (11 of 20) in mice treated with mTREM-1-IgG1 and 15% (3 of 20) in mice treated with control huIgG1 (P = 0.0187, two-tailed Fisher's exact test).
. Figure 23(b) shows the survival curve of C57BL/6 mice that were injected intraperitoneally with mTREM-1-IgG I (open circles), huIgG1 (closed circles) or TNF-RI-IgGI (closed squares) immediately after cecal ligation and puncture (CLP).
Data points are from four independent experiments, which included 5-10 animals per group.
Survival was 45% (18 of 40) in mice treated with mTREM-1-IgGl, 17.5% (7 of 40) in mice treated with control hulgG1 (P = 0.015, two-tailed Fisher's exact test) and 0% (0 of 20) in mice treated with TNF-RI-IgGl.
Figures 24(a)-(d) show the result of FACS analysis demonstrating the specific binding of 29E3 mAb to TREM-2. The 293 cells expressing TREM-2 ((b) and (d)) and those expressing TREM-1 FLAG ((a) and (c)) were compared for staining with 29E3 mAb ((c) and (d)). Expression of TREM- 1 r"G (a) and TREM-2FLA0 (b) was confirmed using anti-FLAG mAbs. The percentages of the cells stained with each mAb (upper right quadrant) are indicated_ Staining with an isotype-matched control mAbs was set to the indicated lower quadrant.
Figures 25(a)-(d) show the result of three-color FACS analysis for TREM-2 expression on monocytes (solid bold line) which were stimulated with M-CSF
(a), GM-CSF
(c), IL-4 (d), or GM-CSF + IL-4 (b) for 36 hours. Dashed profiles indicate background staining with a control IgG1 mAb.
Figures 26(a)-(d) show the three-color FACS analysis for TREM-2 and CD83 expression on monocyte-derived DCs that are stimulated with LPS (b), CD4OL (c), _ .

TNF-a (d), or medium (unstimulated; (a)) for 36 hours. Staining with isotype--matched control mAb was set to the indicated lower quadrants.
Figure 27 shows the result of Western blot analysis under reducing condition in which the surface-biotinylated monocyte-derived DCs lysates were immunoprecipitated with anti-TREM-2 mAb 29E3 (right lanes) or a control IgG1 (anti-TREM-1 mAb 21C7; left lanes). Immunoprecipitates were left untreated or treated with N-glycanase F
and analyzed by Western Blot analysis with Streptavidin-HRP. Deglycosylated TREM-2 is indicated as TREM-2''. Molecular weight markers and specific protein bands are indicated.
Figure 28 shows the result of Western blot analysis in which pervanadate-treated monocyte-derived DCs were subjected to immunoprecipitation with anti-mAb 29E3, or control IgG1 (anti-MHC class-1 mAb). The precipitates were analyzed by anti-phosphotyrosine blot under reducing (left lanes) and nonreducing conditions (right lanes). Tyrosine phosphorylated proteins are marked by arrows. Molecular weight markers are indicated.
Figure 29 shows the result of anti-DAP12 blot analysis, under reducing condition, of a TREM-2 immunoprecipitate from monocyte-derived DCs (left lanes) and monocytes (right lanes) after pervanadate stimulation. TREM-1 immunoprecipitates from monocytes and monocyte-derived DCs were included as positive and negative controls, respectively. Molecular weight markers and specific protein bands are indicated.
Figure 30 shows the functional characterization of Fab and F(ab')2 fragments of anti-TREM-2 mAb 29E30Im. Monocyte-derived DCs were analyzed by FACS for cell surface expression of TREM-2 using either F(ab')2 29E3ai0tin (solid bold profile) or Fab 29E38k6" (grey profile) followed by Streptavidine-PE. TREM-1 is not detectable on monocyte-derived DCs with F(ab')2 9E2Bi fin (solid profile) or Fab 9E2Bk'fin (dashed profile) followed by Streptavidine.
Figures 31(a)-(d) show the result of intracellular calcium measurements in monocyte-derived DCs treated with anti-TREM-1 mAb 21C7 (a) or its F(ab')2 fragments (c), or anti-TREM-2 mAb 29E3 (b) or its F(ab')2 fragments (d). Monovalent engagement of TREM-2 by Fab 29E3B'"Iin is sufficient for induction of Ca2+-flux only in the presence of cross-linking Streptavidine (data not shown).
Figure 32 shows the anti-phosphotyrosine blot of cell lysates from monocyte-derived DCs stimulated with F(ab')2 29E3 (anti-TREM-2) or control F(ab')2 9E2 (anti-TREM-1) for the indicated time periods.
Figures 33(a) and (b) show the result of Western blot in which the lysates of monocyte-derived DCs were stimulated as described for Figure 32 and examined by Western Blot analysis for anti-phospho-Erk 1 and 2 (a) compared to anti-Erk,1-and 2 (b).
Phosphorylated proteins are indicated by arrows. Molecular weight markers are shown.
Figure 34 shows the apoptotic cell death of monocyte-derived DCs that were stimulated with GM-CSF/IL-4 (closed squares), plastic-bound F(ab')2 29E3 (open circles) or control F(ab')2 (closed circles) for the indicated time periods. Apoptotic cell death was determined by measurement of DNA fragmentation.
Figure 35 shows the apoptotic cell death of monocyte-derived DCs that were stimulated as described for Figure 34 in the presence or absence of PD98059 (Erk Inhibitor), LY294002 (PI 3 Kinase Inhibitor) or TPCK (Inhibitor of NFkB-activation).
113 Apoptotic cell death was determined after 8 days by measurement of DNA
fragmentation.
Figure 36 shows the FACS analysis of CCR7 expression on DCs that were stimulated with F(ab')2 control mAb (anti-TREM-1; solid line profiles), F(ab')2 anti-TREM-2 mAb (grey profiles), or LPS (solid bold profiles) for the indicated time periods. Dashed profiles indicate background staining with a control IgM mAb.
Figure 37 shows the DC chemotaxis induced by F(ab')2 anti-TREM-2 mAb.
DCs stimulated for 24 hours with plastic-coated F(ab')2 control mAb (black bars), F(ab')2 anti-TREM-2 mAb (light-grey bars), or LPS (dark-grey bars) were used in transwell chemotaxis assays to assess their chemotactic properties towards medium alone, medium supplemented with 100 ng/ml MIP-313 or ELC. In control experiments, chemotaxis was inhibited by stimulating cells with MIP-313, ELC or anti-CCR7 mAb 10 min prior to the onset of chemotaxis.
Figure 38A-D show the internalization of TREM-2 on monocyte-derived DCs upon ligation. The DCs were incubated with either 1F1 laic" (anti-MHC
class I mAb;
A), 29E3'in (anti-TREM-2 mAb; B), F(ab'), 29E3'in (C), or Fab 29E3B"0tin (D).
The cells were subsequently kept on ice, prepared for total (closed diamonds), extracellular (closed circles), or intracellular receptor (closed squares) staining with a second step Goat-anti mouse IgG-PE or Streptavidine-PE and analyzed by FACS. Numerical values indicate specific mean fluorescence intensity (MFI) after subtraction of the fluorescence detected with an isotype-matched control antibody. The data are representative of 3 independent experiments.
Figure 39(a) and (b) show the result of antigen presentation assay using [3H]thymidine uptake by mouse-IgG1 specific T cell clone as an indicator. In Fig. 39(a), DCs were stimulated with the indicated concentrations of anti-ILT3 mAb (open diamonds), anti-TREM-2 mAb (closed circles), control mAb (open circles), or anti-CD11 b mAb (open squares), whereas, in Fig. 39(b), DCs were stimulated with F(ab') 2 anti-TREM-2 (closed circles) or F(ab')2 control mAb (open circles). F(ab'), anti-TREM-2 was presented to T-cells ¨100-fold more efficiently than was F(ab')2 control mAb. The data are -representative of 3 independent experiments.
5. DETAILED DESCRIPTION OF THE INVENTION
This invention relates generally to new activating receptors of the Ig super-family expressed on human myeloid cells, called TREM (triggering receptor expressed on myeloid cells) which are involved in inflammatory responses. Specifically, this invention relates to TREM-1 and its homologue, TREM-2.
5.1 Human TREM-1 and TREM-2 A cDNA encoding TREM-1 was discovered by its homology to NKp44.
The human TREM-1 cDNA is 884-nucleotide long (Fig. 2; SEQ ID NO:1) and the open reading frame of TREM-1 is nucleotides 48 to 752 of SEQ ID NO:1, which encodes a transmembrane protein comprising the 234 amino acid sequence shown in Fig.
1(a) (SEQ
ID NO:3). Biochemical analysis of TREM-1 by immunoprecipitation and Western blot showed that TREM-I is a glycoprotein of-30 kDa, which is reduced to 26 kDa after N-deglycosylation.
TREM-1 is a novel Ig-SF cell surface molecule which activates neutrophils and monocytes through the transmembrane adapter protein DAP12. TREM-1 induces secretion of inflammatory chemokines and cytokines, release of MPO, and up-regulation of adhesion molecules involved in extravasation. Cellular distribution and functional properties of TREM-1 suggest that it has a role in acute inflammation, which is characterized by an exudate of neutrophils and monocytes. TREM-1-mediated pro-inflammatory responses are potentiated by priming of neutrophils and monocytes with LPS.
Moreover, LPS, bacteria, and fungi up-regulate TREM-1 expression. Thus, TREM-1 and bacterial products induce inflammatory responses via intersecting and mutually stimulating pathways. As discussed in the Examples below, the fusion protein between human IgG1.
constant region and the extracellular domain of mouse TREM-1 (mTREM-1) or that of human TREM-1 (huTREM-1) showed a remarkable protective effect against endotoxemia in mice, demonstrating its therapeutic utility in controlling acute inflammation caused by bacterial infections.
In addition to TREM-1, the present inventors also cloned a novel cDNA
encoding a TREM-I-homologue, called TREM-2_ The cDNA encoding human TREM-2 is 1041-nucleotides long (Fig. 3; SEQ ID NO:2) and the open reading frame of TREM-comprises nucleotides 95 to 787 of SEQ ID NO:2, which encode a transmembrane protein comprising the 230 amino acid sequence shown in Fig. 1(b) (SEQ ID NO:4).
Stimulation of DCs via TREM-2 leads to maturation of DCs which is indicated by upregalation of CD40, CD86 and MHC class II. In addition, TREM-2 stimulation renders DCs resistant against apoptosis and induces strong upregulation of CCR7 and subsequent chemotaxis towards ELC/MIP3-13 (macrophage inflammatory protein 3-13). Thus, TREM-2 regulates DC functions in initiating immune responses by inducing CCR7 expression on peripheral DCs and directing them from the periphery to the draining lymph node.
Furthermore, because TREM-2 is expressed exclusively on DCs but not on granulocytes or monocytes (data not shown), it may stimulate production of constitutive rather than inflammatory chemokines and cytokines. Thus, distinct TREM receptors may regulate acute and chronic inflammatory responses, allowing myeloid cells to mount distinct types of responses to different antigens.
Both TREM-1 and TREM-2 display some sequence homology with activating NK cell receptors, such as NKp44 (Cantoni, C., C. Bottino, M.
Vitale, A.
Pessino, R. Augugliaro, A. Malaspina, S. Parolini, L. Moretta, A. Moretta, and R. Biassoni, is 1999, NKp44, a triggering receptor involved in tumor cell lysis by activated human natural killer cells, is a novel member of the immunoglobulin superfamily. J. Exp.
Med. 189:787).
All of these molecules display a single V-type Ig-like extracellular domain and associate with DAP12 to induce activation. In addition, they are encoded by genes on human chromosome 6. Thus, this chromosome may contain a gene cluster encoding structurally related receptors that activate cell types involved in different innate responses.
As shown in Fig. 1(a) (SEQ ID NO:3), the deduced amino acid sequence of TREM-1 starts with a hydrophobic signal peptide at amino acid residues 1 to 16 of SEQ ID
NO:3 (SEQ ID NO:5) followed by an extracellular region composed of a single Ig-SF
domain, encompassing amino acid residues 17 to 200 of SEQ ID NO:3 (SEQ ID
NO:6), which contain three potential N-glycosylation sites at amino acid residues 146 to 149 of SEQ ID NO:3 (Asn-Ser-Thr-Gln; SEQ ID NO:7), 190 to 193 of SEQ ID NO:3 (Asn-Leu-Thr-Asn; SEQ ID NO:8), and 193 to 196 of SEQ ID NO:3 (Asn-Val-Thr-Asp; SEQ ID
NO:9), and the consensus sequences, Leu-Xaa-Val-Xaa-Cys-Xaa-Tyr (at positions 37-43 of SEQ ID NO:3; "Xaa" indicates any amino acid) and Asp-Xaa-Gly-Xaa-Tyr-Xaa-Cys (at positions 107-113 of SEQ ID NO:3), characteristic of the intrachain disulfide bridge of the Ig-SF V-type fold. The putative transmembrane domain starts from amino acid residues 201 to 229 of SEQ ID NO:3 (SEQ ID NO:10) and contains a charged lysine residue at position 217. Its cytoplasmic tail consists of 5 amino acid residues (SEQ ID
NO:11) and appears to contain no signaling motifs.
TREM-2 (Fig. 1(b); SEQ ID NO:4) starts with a signal peptide at amino acid residues 1 to 13 of SEQ ID NO:4 (SEQ ID NO:12), followed by an extracellular region composed of a single Ig-SF domain, encompassing amino acid residues 14 to 167 of SEQ
ID NO:4 (SEQ ID NO:13), which contains one potential N-glycosylation site at amino acid . residues 20 to 23 of SEQ ID NO:4 (Asn-Thr-Thr-Val; SEQ ID NO:14), and the characteristic Ig-SF consensus sequences at positions 32-38 and 104-110 of SEQ
ID NO:4.
The putative transmembrane domain expands from amino acid residues 168 to 200 of SEQ
ID NO:4 (SEQ ID NO:15) and contains a charged lysine residue at position 186.
Its cytoplasmic tail consists of amino acid residues 201 to 230 of SEQ ID NO:4 (SEQ ID
NO:16).
A "signal sequence" or "signal peptide" as used herein refers to a peptide of at least about 10 to 40 amino acid residues which occurs at the N-terminus of secretory or membrane-bound proteins and contains at least about 50-75% hydrophobic amino acid residues such as alanine, leucine, isoleucine, phenylalanine, proline, tyrosine, tryptophan, or valine. A signal sequence serves to direct a protein containing such a sequence to a lipid bilayer. A signal sequence is usually cleaved during the maturation process of the protein.
Thus, the invention also includes the domains and the mature protein resulting from cleavage of such a signal peptide.
Accordingly, a mature TREM comprises one or more of the following domains: (1) an extracellular domain which contains at least one Ig-SF domain;
(2) a transmembrane domain; and (3) a cytoplasmic domain.
Thus, in one embodiment, a polypeptide of the invention comprises the amino acid sequence of SEQ ID NO:3 or 4. In another embodiment, a polypeptide of the invention is a mature polypeptide which does not contain a signal peptide and comprises amino acid residues 17 to 234 of SEQ ID NO:3 (SEQ ID NO:17) or amino acid residues 14 to 230 of SEQ ID NO:4 (SEQ ID NO:18). In another aspect, a polypeptide of the invention comprises the amino acid sequence of SEQ ID NO:3 or 4 except that amino acid residues 1 to 16 of SEQ ID NO:3 or 1 to 13 of SEQ ID NO:4 are replaced by a heterologous signal peptide by genetic engineering.
Yet, in another embodiment, a polypeptide of the invention comprises an extracellular domain comprising amino acid residues 17 to 200 of SEQ ID NO:3 (SEQ ID
NO:6) or amino acid residues 14 to 167 of SEQ ID NO:4 (SEQ ID NO:13). In another embodiment, a polypeptide of the invention comprises a transmembrane domain comprising amino acid residues 201 to 229 of SEQ ID NO:3 (SEQ ID NO:10) or amino acid residues 168 to 200 of SEQ ID NO:4 (SEQ ID NO:15).
Further, a polypeptide of the invention comprises a cytoplasmic domain comprising amino acid residues 230 to 234 of SEQ ID NO:3 (SEQ ID NO:11) or amino acid residues 201 to 230 of SEQ ID NO:4 (SEQ ID NO:12).

In preferred embodiments, a polypeptide of the invention compi ___ ises a fragment of SEQ ID NO:3 or 4 which exhibits at least one structural and/or functional feature of TREM-1 or TREM-2, wherein said functional feature includes a capability of eliciting a specific immune response, such as producing anti-TREM-1 or anti-antibodies or ability to immunospecifically bind anti-TREM-1 or anti-TREM-2 antibodies.
Included within the present invention is an isolated nucleic acid molecule that encodes a polypeptide of the invention having the amino acid sequence of SEQ ID
NO:3, 4, 5, 6, 10, 11, 12, 13, 15, 16, 17, or 18, or a complement thereof. The nucleic acid molecules of the invention include the entire or a portion (of at least 5, 10, 15, 20, 25, 50, 75, 100, 150, 200, 250, 300, 350, 400, 450, 500, 550, 600, 650 or more contiguous nucleotides) of the nucleotide sequence of SEQ ID NO:! or 2, that is, SEQ ID
NO:1, 2, 19, 20, 21, 22, 23, 24, 25, 26, 27, or 28, or a complement thereof, respectively, which corresponds to the nucleotide sequence encoding the amino acid sequence of SEQ
ID NO:3, 4, 5, 6, 10, 11, 12, 13, 15, 16, 17, or 18, respectively. Furthermore, because of the genetic code degeneracy, the invention also includes nucleic acid molecules that are different from SEQ ID NO:1, 2, 19, 20, 21, 22, 23, 24, 25, 26, 27, or 28, but encode the amino acid sequence of SEQ ID NO:3, 4, 5, 6, 10, 11, 12, 13, 15, 16, 17, or 18.
5.2 Homologues, Variants, and Derivatives of TREM-1 and TREM-2 In addition to the nucleic acid molecules and polypeptides described above, nucleic acid molecules or polypeptides of the invention also encompass those nucleic acid molecules and polypeptides having a common biological activity and/or structural domain and having sufficient nucleotide sequence or amino acid identity (homologues) as defined herein. These homologues can be from either the same or different species of animal, preferably from mammals, more preferably from rodents, such as mouse and rat, and most preferably from human. Preferably, they exhibit at least one structural and/or functional feature of TREM-1 or TREM-2, including antigenicity/immunogenicity.
Homologues of the nucleic acid molecules of the invention can be isolated based on their close identity to the human nucleic acid molecules disclosed herein, by standard hybridization techniques under stringent or moderately stringent conditions, as defined herein below, using the human cDNA of the invention or a portion thereof as a hybridization probe.
Accordingly, the invention also includes an isolated nucleic acid molecule being at least 25, 50, 100, 200, 300, 400, 500, 600, 700, 800, 900, or 1000 contiguous nucleotides in length and hybridizing under stringent or moderately stringent conditions to = the nucleic acid molecule comprising the nucleotide sequence of SEQ ID
NO:I, 2, 19, 20, 21, 22, 23, 24, 25, 26, 27, or 28, or a complement thereof.
The term "under stringent condition" refers to hybridization and washing conditions under which nucleotide sequences having at least 60%, preferably 65%, more preferably 70%, most preferably 75% identity to each other remain hybridized to each other.
The term "moderately stringent condition" refers to hybridization and washing conditions under which nucleotide sequences having at least 40%, preferably 45%, more preferably 50%, most preferably 55% identity to each other remain hybridized to each other. Such hybridization conditions are described in, for example but not limited to, Current Protocols in Molecular Biology, John Wiley & Sons, N.Y. (1989), 6.3.1-6.3.6., and Basic Methods in Molecular Biology, Elsevier Science Publishing Co., Inc., N.Y. (1986), pp. 75-78, and 84-87, and are well known to those skilled in the art. A preferred, non-limiting example of stringent hybridization conditions is hybridization in 6X sodium chloride/sodium citrate (SSC) at about 45 C followed by one or more washes in 0.2X SSC, 0.1% SDS at about 50-65 C. A preferred, non-limiting example of moderately stringent conditions is hybridization in 6X SSC at about 42 C followed by one or more washes in 0.2X
S.C., 0.1%
SDS at about 45-55 C.
In another aspect, an isolated nucleic acid molecule of the invention encodes a variant of a polypeptide of the invention in which the amino acid sequences have been modified by genetic engineering in order to either enhance or reduce biological activities of the polypeptides, or change the local structures thereof without significantly altering the biological activities. In one aspect, these variants can act as either agonists or as antagonists. An agonist can retain substantially the same or a portion of the biological activities of the polypeptides of the invention and an antagonist can inhibit one or more of the activities of the polypeptides of the invention. Such modifications include amino acid substitution, deletion, and/or insertion. Amino acid modifications can be made by any method known in the art and various methods are available to and routine for those skilled in the art.
For example, mutagenesis may be performed in accordance with any of the techniques known in the art including, but not limited to, synthesizing an oligonucleotide having one or more modifications within the sequence of a given polypeptide to be modified. Site-specific mutagenesis can be conducted using specific oligonucleotide sequences which encode the nucleotide sequence containing the desired mutations in addition to a sufficient number of adjacent nucleotides in the polypeptide.
Such oligonucleotides can serve as primers which can form a stable duplex on both sides of the deletion junction being traversed. Typically, a primer of about 17 to about 75 nucleotides or more in length is preferred, with about 10 to about 25 or more residues on'both sides of the junction of the sequence being altered. A number of such primers introducing a variety of different mutations at one or more positions may be used to generated a library of mutants.
The technique of site-specific mutagenesis is well known in the art, as described in various publications (e.g.,. Kunkel etal., Methods Enzymol., 154:367-82, 1987 In general, site-directed mutagenesis is performed by first obtaining a single-stranded vector or melting apart of two strands of a double stranded vector which includes within its sequence a DNA
sequence which encodes the desired peptide. An oligonucleotide primer bearing the desired mutated sequence is prepared, generally synthetically. This primer is then annealed with the single-stranded vector, and subjected to DNA polymerizing enzymes such as T7 DNA
polymerase, in order to complete the synthesis of the mutation-bearing strand.
Thus, a heteroduplex is formed wherein one strand encodes the original non-mutated sequence and the second strand bears the desired mutation. This heteroduplex vector is then used to transform or transfect appropriate cells, such as E. colt cells, and clones are selected which include recombinant vectors bearing the mutated sequence arrangement. As will be appreciated, the technique typically employs a phage vector which exists in both a single stranded and double stranded form. Typical vectors useful in site-directed mutagenesis include vectors such as the M13 phage. These phage are readily commercially available and their use is generally well known to those skilled in the art. Double stranded plasmids are also routinely employed in site directed mutagenesis which eliminates the step of transferring the gene of interest from a plasmid to a phage.
Alternatively, the use of PCRTM with commercially available thermostable enzymes such as Tay DNA polymerase may be used to incorporate a mutagenic oligonucleotide primer into an amplified DNA fragment that can then be cloned into an appropriate cloning or expression vector_ See, e.g., Tomic et al., Nucleic Acids Res., 18(6):1656, 1987, and Upender et al., Biotechniques, 18(1):29-30, 32, 1995, for PCRTm -mediated mutagenesis procedures.
PCRTm employing a thennostable ligase in addition to a therrnostable polymerase may also be used to incorporate a phosphorylated mutagenic oligonucleotide into an amplified DNA fragment that may then be cloned into an appropriate cloning or expression vector (see e.g., Michael, Biotechniques, 16(3):410-2, 1994 )t.
Other methods known to those skilled in art of producing sequence variants of a given polypeptidc or a fragment thereof (e.g., an extracellular-domain, transmembrane-domain, and cytoplasmic-domain fragments) can be used. For example, recombinant vectors encoding the amino acid sequence of the polypeptide or a fragment thereof may be treated with mutagenic agents, such as hydroxylamine, to obtain sequence variants.
Preferably, the amino acid residues to be modified are surface exposed residues. Additionally, in making amino acid substitutions, preferably the amino acid residue to be substituted is a conservative amino acid substitution, for example, a polar residue is substituted with a polar residue, a hydrophilic residue with a hydrophilic residue, hydrophobic residue with a hydrophobic residue, a positively charged residue with a positively charged residue, or a negatively charged residue with a negatively charged residue. Moreover, preferably, the amino acid residue to be modified is not highly or completely conserved across species and/or is critical to maintain the biological activities of the protein.
Accordingly, included in the scope of the invention are nucleic acid molecules encoding a polypeptide of the invention that contains amino acid modifications that are not critical to activity. Thus, an isolated nucleic acid molecule of the invention includes a nucleotide sequence encoding a polypeptide having an amino acid sequence that is at least about 40%, 45%, 50%, 55%, 60%, 65%, 70%, 75%, 80%, 85%, 90%, 95%, or 98% identical to the amino acid sequence of SEQ ID NO:3, 4, 5, 6, 10, 11, 12, 13, 15, 16, 17, or 18.
Furthermore, the invention also encompasses derivatives of the polypeptides of the invention. For example, but not by way of limitation, derivatives may include peptides or proteins that have been modified, e.g., by glycosylation, acetylation, pegylation, phosphorylation, amidation, derivatization by known protecting/blocking grOups, proteolytic cleavage, linkage to a cellular ligand or other protein, etc. Any of numerous chemical modifications may be carried out by known techniques, including, but not limited to, specific chemical cleavage, acetylation, formylation, etc. Additionally, the derivative may contain one or more non-classical amino acids.
In another aspect, the invention further includes antisense nucleic acid molecules which are complementary to an entire or partial sense nucleic acid encoding a polypeptide of the invention (e.g., a coding strand of cDNA or a mRNA). The antisense nucleic acid molecules can also be complementary to non-coding region of the nucleic acid which will not be translated. The antisense nucleic acid molecules of the invention can be administered to a subject so that they hybridize with cellular mRNA or genomic DNA
which encodes a polypeptide of the invention. This blocks the transcription and/or translation of the target sequence and, thereby inhibits expression of the polypeptide. An antisense nucleic acid may be about 5, 10, 15, 20, 25, 30, 35, 40, 45, or 50 nucleotide long or longer and can be prepared by chemical synthesis and enzymatic ligation reactions using methods well known in the art. For, example, an antisense nucleic acid can tre-chemically synthesized using naturally occurring nucleotides or variously modified nucleotides designed to increase the biological stability of the molecules or to increase the physical stability of the duplex formed between the antisense and sense nucleic acids;
for example, phosphorothioate derivatives and acridine substituted nucleotides can be used.
Examples of modified nucleotides which can be used to generate the antisense nucleic acid include 5-fluorouracil, 5-bromouraci1, 5-chlorouracil, 5-iodouracil, hypoxanthine, xanthine, 4-acetylcytosine, 5-(carboxyhydroxylmethyl) uracil, 5-carboxymethylaminomethy1-2-thiouridine, 5-carboxymethylaminomethyluraci1, dihydrouracil, beta-D-galactosylqueosine, inosine, N6-isopentenyladenine, 1-methylguanine, 1-methylinosine, 2,2-dimethylguanine, 2-methyladenine, 2-methylguanine, 3-methylcytosine, 5-methylcytosine, N6-adenine, 7-methylguanine, 5-methylaminomethyluracil, 5-methoxyaminomethy1-2-thiouracil, beta-D-mannosylqueosine, 5'-methoxycarboxymethyluracil, 5-methoxyuraci1, 2-methylthio-N6-isopentenyladenine, uracil-5-oxyacetic acid (v), wybutoxosine, pseudouracil, queosine, 2-thiocytosine, 5-methyl-2-thiouracil, 2-thiouracil, 4-thiouracil, 5-methyluracil, uracil-5-oxyacetic acid methylester, uracil-5-oxyacetic acid (v), 5-methyl-2-thiouracil, 3-(3-amino-3-N-2-carboxypropyl) uracil, (acp3)w, and 2,6-diaminopurine. Alternatively, the antisense nucleic acid can be produced biologically using an expression vector into which a nucleic acid has been subcloned in an antisense orientation (i.e., RNA transcribed from the inserted nucleic acid will be of an antisense orientation to a target nucleic acid of interest).
The antisense nucleic acid molecules of the invention are typically administered to a subject or generated in situ such that they hybridize with or bind to cellular mRNA and/or genomic DNA encoding a selected polypeptide of the invention to thereby inhibit expression, e.g., by inhibiting transcription and/or translation. The hybridization can be by conventional nucleotide complementarity to form a stable duplex, or, for example, in the case of an antisense nucleic acid molecule which binds to DNA
duplexes, through specific interactions in the major groove of the double helix. An example of a route of administration of antisense nucleic acid molecules of the invention includes direct injection at a tissue site. Alternatively, antisense nucleic acid molecules can be modified to target selected cells and then administered systemically. For example, for systemic administration, antisense molecules can be modified such that they specifically bind to receptors or antigens expressed on a selected cell surface, e.g., by linking the antisense nucleic acid molecules to peptides or antibodies which bind to cell surface receptors or antigens. The antisensc nucleic acid molecules can also be delivered to cells using the vectors described herein. To achieve sufficient intracellular concentrations of the antisense molecules, vector constructs in which the antisense nucleic acid molecule is placed under the control of a strong poi 11 or pol III promoter are preferred.
An antisense nucleic acid molecule of the invention can be an a-anomeric nucleic acid molecule. An a-anomeric nucleic acid molecule forms specific double-stranded hybrids with complementary RNA in which, contrary to the usual 0-units, the strands run parallel to each other (Gaultier et al. (1987) Nucleic Acids Res.
15:6625-6641). The antisense nucleic acid molecule can also comprise a 2'-o-methylribonucleotide (Inoue et al, (1987) Nucleic Acids Res. 15:6131-6148) or a chimeric RNA-DNA analogue (Inoue et al. (1987) FEBS Lett. 215:327-330).
The invention also encompasses ribozymes. Ribozymes are catalytic RNA
molecules with ribonuclease activity which are capable of cleaving a single-stranded nucleic acid, such as an mRNA, to which they have a complementary region. Thus, ribozymes, such as hammerhead ribozymes (described in Haselhoff and Gerlach, 1988, Nature, 334:585-591) can be used to catalytically cleave mRNA transcripts to thereby inhibit translation of the protein encoded by the mRNA. A ribozyme having specificity for a nucleic acid molecule encoding a polypeptide of the invention can be designed based upon the nucleotide sequence of a cDNA. For example, a derivative of a Tetrahymena RNA can be constructed in which the nucleotide sequence of the active site is complementary to the nucleotide sequence to be cleaved (Cech et al., U.S.
Patent No.
4,987,071; and Cech et aL, U.S. Patent No. 5,116,742). Alternatively, an mRNA
encoding a polypeptide of the invention disclosed herein can be used to select a catalytic RNA having a specific ribonuclease activity from a pool of RNA molecules. See, e.g., Bartel and Szostak, 1993, Science, 261:1411-1418.
The invention also encompasses nucleic acid molecules which form triple helical structures. For example, expression of a polypeptide of the invention can be inhibited by targeting nucleotide sequences complementary to the regulatory region of the gene encoding the polypeptide (e.g., the promoter and/or enhancer) to form triple helical structures that prevent transcription of the gene in target cells. See generally Helene, 1991, Anticancer Drug Des., 6(6):569-84; Helene, 1992, Ann. N.Y. Acad. Sci., 660:27-36; and Maher, 1992, Bioassays, 14(12):807-15.
In various embodiments, the nucleic acid molecules of the invention can be modified at the base moiety, sugar moiety or phosphate backbone to improve, e.g., the stability, hybridization, or solubility of the molecule. For example, the deoxyribose phosphate backbone of the nucleic acids can be modified to generate peptide nucleic acids (see Hyrup et al., 1996, Bioorganic & Medicinal Chemistry, 4(1): 5-23). As used herein, the terms "peptide nucleic acids" or "PNAs" refer to nucleic acid mimics, e_g:,-DNA
mimics, in which the deoxyribose phosphate backbone is replaced by a pseudopeptide backbone and only the four natural nucleobases are retained. The neutral backbone of PNAs has been shown to allow for specific hybridization to DNA and RNA under conditions of low ionic strength. The synthesis of PNA oligomers can be performed using standard solid phase peptide synthesis protocols as described in Hyrup et al., supra;
Perry-O'Keefe et at., 1996, Proc. Natl. Acad. Sci. USA, 93: 14670-675.
PNAs can be used in therapeutic and diagnostic applications. For example, PNAs can be used as antisense or antigene agents for sequence-specific modulation of gene expression by, e.g., inducing transcription or translation arrest or inhibiting replication.
PNAs can also be used, e.g., in the analysis of single base pair mutations in a gene by, e.g., PNA directed PCR clamping; as artificial restriction enzymes when used in combination with other enzymes, e.g., S1 nucleases (Hyrup, supra); or as probes or primers for DNA
sequence and hybridization (Hyrup, supra; Perry-O'Keefe et at., supra).
In another embodiment, PNAs can be modified, e.g., to enhance their stability or cellular uptake, by attaching lipophilic or other helper groups to PNA, by the formation of PNA-DNA chimeras, or by the use of liposomes or other techniques of drug delivery known in the art. For example, PNA-DNA chimeras can be generated which may combine the advantageous properties of PNA and DNA. Such chimeras allow DNA
recognition enzymes, e.g., RNAse H and DNA polymerases, to interact with the DNA
portion while the PNA portion would provide high binding affinity and specificity.
PNA-DNA chimeras can be linked using linkers of appropriate lengths selected in terms of base stacking, number of bonds between the nucleobases, and orientation (Hyrup, supra).
The synthesis of PNA-DNA chimeras can be performed as described in Hyrup, supra, and Finn etal. (1996, Nucleic Acids Res., 24(17):3357-63). For example, a DNA
chain can be synthesized on a solid support using standard phosphoramidite coupling chemistry and modified nucleoside analogs. Compounds such as 5'-(4-rnethoxytrityl)amino-5'-deoxy-thymidine phosphoramidite can be used as a link between the PNA and the 5' end of DNA (Mag et al., 1989, Nucleic Acids Res., 17:5973-88). PNA monomers are then coupled in a stepwise manner to produce a chimeric molecule with a 5' PNA segment and a 3' DNA segment (Finn et al., 1996, Nucleic Acids Res., 24(17):3357-63). Alternatively, chimeric molecules can be synthesized with a 5' DNA
segment and a 3' PNA segment (Peterser et al., 1975, Bioorganic Med. Chem.
Lett., 5:1119-11124).
In other embodiments, the oligonucleotide may include other appended groups such as peptides (e.g., for targeting host cell receptors in vivo ), or agents facilitating transport across the cell membrane (see, e.g., Letsinger et al., 1989, Proc.
Nall: Acad. Sci.
USA, 86:6553-6556; Lemaitre et al., 1987, Proc. Natl. Acad. Sci. USA, 84:648-652; PCT
Publication No. WO 88/09810) or the blood-brain barrier (see, e.g., PCT
Publication No.
WO 89/10134). In addition, oligonucleotides can be modified with hybridization-triggered cleavage agents (see, e.g., Krol et al.,1988, Bio/Techniques, 6:958-976) or intercalating agents (see, e.g., Zon, 1988, Phartn. Res., 5:539-549). To this end, the oligonucleotide may be conjugated to another molecule, e.g., a peptide, hybridization triggered cross-linking agent, transport agent, hybridization-triggered cleavage agent, etc.
5.3 Recombinant expression vectors and host cells The present invention also provides vectors, preferably expression vectors, containing a nucleic acid encoding a polypeptide of the invention or a portion thereof. As used herein, the term "vector" refers to a nucleic acid molecule capable of transporting another nucleic acid to which it has been linked. One type of vector is a "plasmid", which refers to a circular double stranded DNA loop into which additional DNA
segments can be ligated. Another type of vector is a viral vector, wherein additional DNA
segments can be ligated into the viral genome. Certain vectors are capable of autonomous replication in a host cell into which they are introduced (e.g., bacterial vectors having a bacterial origin of replication and episomal mammalian vectors). Other vectors (e.g., non-episomal 90 mammalian vectors) are integrated into the genome of a host cell upon introduction into the host cell, and thereby are replicated along with the host genome. Moreover, certain expression vectors, are capable of directing the expression of genes to which they are operably linked. In general, expression vectors of utility in recombinant DNA
techniques are often in the form of plasmids (vectors). However, the invention also includes other forms of expression vectors, such as viral vectors (e.g., replication defective retroviruses, adenoviruses and adeno-associated viruses), which serve equivalent functions.
The recombinant expression vectors of the invention comprise a nucleic acid of the invention in a form suitable for expression of the nucleic acid in a host cell. In other words, the recombinant expression vectors include one or more regulatory sequences, preferably heterologous to the nucleic acid of the invention, which are selected on the basis of the host cells to be used for expression and are operably linked to the nucleic acid sequence to be expressed. Within a recombinant expression vector, "operably linked" is intended to mean that the nucleotide sequence of interest is linked to the regulatory sequence(s) in a manner which allows for expression of the nucleotide sequence (e.g., in an in vitro transcription/translation system or in a host cell when the vector is introduced into the host cell). The term "regulatory sequence" is intended to include promoters, enhancers and other expression control elements (e.g., polyadenylation signals). Such regulatory sequences are described, for example, in Goeddel, Gene Expression Technology:
Methods in Enzymology 185, Academic Press, San Diego, CA (1990). Regulatory sequences include those which direct constitutive expression of a nucleotide sequence in many types of host cell and those which direct expression of the nucleotide sequence only in certain host cells (e.g., tissue-specific regulatory sequences). It will be appreciated by those skilled in the art that the design of the expression vector can depend on such factors as the choice of the host cell to be transformed, the level of expression of protein desired, etc. The expression vectors of the invention can be introduced into host cells to thereby produce proteins or peptides, including fusion proteins or peptides, encoded by nucleic acids as described herein.
A variety of host-vector systems may be utilized in the present invention to express the protein-coding sequence. These include but are not limited to bacteria transformed with bacteriophage, DNA, plasmid DNA, or cosmid DNA;
microorganisms such as yeast containing yeast vectors; insect cell systems infected with virus (e.g., baculovirus); or mammalian cell systems infected with virus (e.g., vaccinia virus, adenovirus, etc.). The expression elements of vectors vary in their strengths and specificities. Depending on the host-vector system utilized, any one of a number of suitable transcription and translation elements may be used. Suitable host cells are discussed further in Goeddel, supra. Alternatively, the recombinant expression vector can be transcribed and translated in vitro, for example using T7 promoter regulatory sequences and T7 polymerase.
Expression of proteins in prokaryotes is most often carried out in E. coli with vectors containing constitutive or inducible promoters directing the expression of either fusion or non-fusion proteins. Fusion vectors add a number of amino acids to a protein encoded therein, usually to the amino terminus of the recombinant protein.
Such fusion vectors typically serve three purposes: 1) to increase expression of recombinant protein; 2) to increase the solubility of the recombinant protein; and 3) to aid in the purification of the recombinant protein by acting as a ligand in affinity purification. Often, in fusion expression vectors, a proteolytic cleavage site is introduced at the junction of the fusion moiety and the recombinant protein to enable separation of the recombinant protein from the fusion moiety subsequent to purification of the fusion protein. Such enzymes, and their cognate recognition sequences, include Factor Xa, thrombin and enterokinase.
Typical fusion expression vectors include pGEX (Phannacia Biotech Inc; Smith and Johnson (1988) Gene 67:31-40), pMAL (New England Biolabs, Beverly, MA) and pRIT5 (Pharmacia, Piscataway, NJ) which fuse glutathione S-transferase (GST), maltose E binding protein, or protein A, respectively, to the target recombinant protein. Fusion proteins comprising a polypeptide of the invention are further discussed in section 5.4 below.
Examples of suitable inducible non-fusion E. coli expression vectors include pTrc (Amann et al., 1988, Gene 69:301-315) and pET lid (Studier et al., Gene Expression Technology: Methods in Enzymology, 185, Academic Press, San Diego, California, 1990, pp. 60-89). Target gene expression from the pTrc vector relies on host RNA
polymerase TM
transcription from a hybrid trp-lac fusion promoter. Target gene expression from the pET
1Id vector relies on transcription from a T7 gni 0-lac fusion promoter mediated by a coexpressed viral RNA polymerase (T7 gnl). This viral polymerase is supplied by host strains BL21(DE3) or HMSI74(DE3) from a resident X prophage harboring a T7 gni gene under the transcriptional control of the IacUV 5 promoter.
One strategy to maximize recombinant protein expression in E. coil is to express the protein in a host bacteria with an impaired capacity to proteolytically cleave the recombinant protein (Gottesman, Gene apression Technology: Methods in Enzymology 185, Academic Press, San Diego, California, 1990, pp. 119-128). Another strategy is to alter the nucleic acid sequence of the nucleic acid to be inserted into an expression vector so that the individual codons for each amino acid are those preferentially utilized in E. coil (Wada et al., 1992, Nucleic Acids Res., 20:2111-2118). Such alteration of nucleic acid sequences of the invention can be carried out by standard DNA synthesis techniques.
In another embodiment, the expression vector is a yeast expression vector.
Examples of vectors for expression in yeast S. cerevisiae include pYepSecl (Baldari et aL, 1987, Ekl.B0 J., 6:229-234), pMFa (Kurjan and Herskowitz, 1982, Cell, 30:933-943), pJRY88 (Schultz et al., 1987, Gene, 54:113-123), pYES2 (1nvitrogen Corporation, San Diego, CA), and pPicZ (lnvitrogen Corp, San Diego, CA).
Alternatively, the expression vector is a baculovirus expression vector.
Baculovirus vectors available for expression of proteins in cultured insect cells (e.g., Sf 9 cells) include the pAc series (Smith et al., 1983, MoL Cell Biol., 3:2156-2165) and the pVL
series (Lucklow and Summers, 1989, Virology, 170:31-39).
In yet another embodiment, a nucleic acid of the invention is expressed in mammalian cells using a mammalian expression vector. Examples of mammalian expression vectors include pCDM8 (Seed, 1987, Nature, 329:840) and pMT2PC
(Kaufman et al., 1987, EMBO J., 6:187-195). When used in mammalian cells, the expression vector's control functions are often provided by viral regulatory elements. For example, commonly used promoters are derived from polyoma,.adenovirus 2, cytomegalovirus and Simian Virus 40. For other suitable expression systems for both prokaryotic and eukaryotic cells see chapters 16 and 17 of Sambrook et al., (1990, Molecular Cloning, A Laboraton, Manual, 2d Ed., Cold Spring Harbor Laboratory, Cold Spring Harbor, NY).
In another embodiment, the recombinant mammalian expression vector is capable of directing expression of the nucleic acid preferentially in a particular cell type (e.g., tissue-specific regulatory elements are used to express the nucleic acid).
Tissue-specific regulatory elements are known in the art. Non-limiting examples of suitable tissue-specific promoters include the albumin promoter (liver-specific;
Pinkert etal., 1987, Genes Dev.,1:268-277), lymphoid-specific promoters (Calame and Eaton, 1988, Adv.
linntunol., 43:235-275), in particular promoters of T cell receptors (Winoto and Baltimore, 1989, EMBO J., 8:729-733) and immunoglobulins (Banerji etal., 1983, Cell, 33:729-740;
Queen and Baltimore, 1983, Cell, 33:741-748), neuron-specific promoters (e.g., the neurofilament promoter; Byrne and Ruddle, 1989, Proc. Natl. Acad. Sci. USA, 86:5473-5477), pancreas-specific promoters (Edlund etal., 1985, Science, 230:912-916), and mammary gland-specific promoters (e.g., milk whey promoter; U.S. Patent No.
4,873,316 and European Application Publication No. 264,166). Developmentally-regulated promoters are also encompassed, for example the murine hox promoters (Kessel and Gruss, 1990, Science, 249:374-379) and the cc-fetoprotein promoter (Campes and Tilghman, 1989, Genes Dev., 3:537-546).
The invention further provides a recombinant expression vector comprising a DNA molecule of the invention cloned into the expression vector in an antisense orientation. That is, the DNA molecule is operably linked to a regulatory sequence in a manner which allows for expression (by transcription of the DNA molecule) of an RNA
molecule which is antisense to the mRNA encoding a polypeptide of the invention.
Regulatory sequences operably linked to a nucleic acid cloned in the antisense orientation can be chosen which direct the continuous expression of the antisense RNA
molecule in a variety of cell types, for instance viral promoters and/or enhancers, or regulatory sequences can be chosen which direct constitutive, tissue specific or cell type specific expression of antisense RNA. The antisense expression vector can be in the form of a recombinant plasmid, phagemid or attenuated virus in which antisense nucleic acids are produced under the control of a high efficiency regulatory region, the activity of which can be determined by the cell type into which the vector is introduced. For a discussion of the regulation of gene expression using antisense genes see Weintraub et al. (Reviews - Trends in Genetics, Vol. 1(1), 1986).
Another aspect of the invention pertains to host cells into which a recombinant expression vector of the invention has been introduced. The terms "host cell"
and "recombinant host cell" are used interchangeably herein. It is understood that such terms refer not only to the particular subject cell but to the progeny or potential progeny of such a cell. Because certain modifications may occur in succeeding generations due to either mutation or environmental influences, such progeny may not be identical to the parent cell, but are still included within the scope of the tenn as used herein_ A host cell can be any prokaryotic (e.g., E. coil) or eukaryotic cell (e.g., insect cells, yeast or mammalian cells). A host cell strain may be selected which modulates the expression of the inserted sequences, or modifies and processes the gene product in the specific fashion desired. Expression from certain promoters can be elevated in the presence of certain inducers; thus, expression of the genetically engineered polypeptide/protein may be controlled. Furthermore, different host cells have characteristic and specific mechanisms for the translational and post-translational processing and modification (e.g., glycosylation, phosphorylation of proteins). Appropriate cell lines or host systems can be chosen to ensure the desired modification and processing of the foreign protein expressed. For example, expression in a bacterial system will produce an unglycosylated product and expression in yeast will produce a glycosylated product. Eukaryotic host cells which possess the cellular machinery for proper processing of the primary transcript, glycosylation, and phosphorylation of the gene product may be used. Such mammalian host cells include but are not limited to CHO, VERY, BHK, HeLa, COS, MDCK, 293, 3T3, W138, and in particular, neuronal cell lines such as, for example, SK-N-AS, SK-N-FI, SK-N-DZ human neuroblastomas (Sugimoto et al., 1984,1 Natl, Cancer Inst., 73:51-57), SK-N-SH
human neuroblastoma (Biochini. Biophys. Ada, 1982, 704:450-460), Daoy human cerebellar medulloblastoma (He et al., 1992, Cancer Res., 52:1144-1148) DBTRG-05MG
glioblastoma cells (Kruse et al., 1992, In Vitro Cell. Dev. Biol., 28A:609-614), IMR-32 human neuroblastoma (Cancer Res., 1970, 30:2110-2118), 1321N1 human astrocytoma (Proc. Nail Acad. Sci. USA,1977, 74:4816), MOG-G-CCM human astrocytoma (Br.
J.
Cancer, 1984, 49:269), U87MG human glioblastoma-astrocytoma (Acta PathoL
MicrobioL
Scand., 1968, 74:465-486), A172 human glioblastoma (Olopade et al., 1992, Cancer Res., 52:2523-2529), CO rat glioma cells (Benda etal., 1968, Science, 161:370-371), Neuro-2a mouse neuroblastoma (Proc. Natl. Acad. Sci. USA, 1970, 65:129-136), NB41A3 mouse neuroblastoma (Proc. Natl. Acad. Sci. USA, 1962, 48:1184-1190), SCP sheep choroid plexus (Bolin etal., 1994,1 ViroL Methods, 48:211-221), G355-5, PG-4 Cat normal astrocyte (Haapala etal., 1985,]. ViroL, 53:827-833), Mpf ferret brain (Trowbridge etal., 1982, In Vitro, 18:952-960), and normal cell lines such as, for example, CTX
TNA2 rat normal cortex brain (Radany etal., 1992, Proc. Natl. Acad. Sci. USA, 89:6467-6471) such as, for example, CRL7030 and Hs578Bst. Furthermore, different vector/host expression systems may effect processing reactions to different degrees.
=

Vector DNA can be introduced into prokaryotic or eukaryotic tells via conventional transformation or transfection techniques. As used herein, the terms "transformation" and "transfection" are intended to refer to a variety of art-recognized techniques for introducing foreign nucleic acid into a host cell, including calcium phosphate or calcium chloride co-precipitation, DEAE-dextran-mediated transfection, lipofection, or electroporation. Suitable methods for transforming or transfecting host cells can be found in Sambrook, et al., supra, and other laboratory manuals.
For stable transfection of mammalian cells, it is known that, depending upon the expression vector and transfection technique used, only a small fraction of cells may integrate the foreign DNA into their genome. In order to identify and select these integrants, a gene that encodes a selectable marker is generally introduced into the host cells along with the gene of interest. A number of selection systems may be used, including but not limited to the herpes simplex virus thymidine kinase (Wigler, etal., 1977, Cell, 11:223), hypoxanthine-guanine phosphoribosyltransferase (Szybalska & Szybalski, 1962, Proc. Natl.
Acad. Sci. USA, 48:2026), and adenine phosphoribosyltransferase (Lowy, et al., 1980, Cell, 22:817) genes can be employed in tk-, hgprt- or aprt- cells, respectively.
Also, antimetabolite resistance can be used as the basis of selection for dhfr, which confers resistance to methotrexate (Wigler, et al., 1980, Natl. Acad. Sci. USA, 77:3567; O'Hare, et al., 1981, Proc. Natl. Acad. Sci. USA, 78:1527); gpt, which confers resistance to mycophenolic acid (Mulligan & Berg, 1981, Proc. Natl. Acad. Sci. USA, 78:2072); neo, which confers resistance to the aminoglycoside G-418 (Colberre-Garapin, etal., 1981,1 Mol. Biol., 150:1); and hygro, which confers resistance to hygromycin (Santerre, etal., 1984, Gene, 30:147) genes.
In another embodiment, the expression characteristics of an endogenous gene sequence (e.g., TREM-1 and TREM-2) within a cell, cell line or cloned microorganism may be modified by inserting a DNA regulatory element heterologous to the endogenous gene of interest into the genome of a cell, stable cell line or cloned microorganism such that the inserted regulatory element is operatively linked with the endogenous gene and controls, modulates or activates the endogenous gene. For example, endogenous TREM-1 and TREM-2 which are expressed only at very low levels in a cell or cell line, may be activated by inserting a regulatory element which is capable of promoting the expression of a normally expressed gene product in that cell or cell line. Alternatively, endogenous TREM-1 and TREM-2 genes which are normally "transcriptionally silent", i.e., TREM-I
and TREM-2 genes which are normally not expressed, may be activated by insertion of a promiscuous regulatory element that works across cell types.

A heterologous regulatory element may be inserted into a stabie cell line or cloned microorganism, such that it is operatively linked with and activates expression of endogenous TREM-1 and TREM-2 genes, using techniques, such as targeted homologous recombination, which are well known to those skilled in the art, and described, for example, in Chappel, U.S. Patent No. 5,272,071; PCT publication No. WO 91/06667 (published May 16, 1991).
A host cell of the invention, such as a prokaryotic or eukaryotic host cell in culture, can be used to produce a polypeptide of the invention. Accordingly, the invention further provides methods for producing a polypeptide of the invention using the host cells of the invention. In one embodiment, the method comprises culturing the host cell of the invention, into which a recombinant expression vector encoding a polypeptide of the invention has been introduced, in a suitable medium such that the polypeptide is produced.
In another embodiment, the method further comprises isolating the polypeptide from the medium or the host cell.
The host cells of the invention can also be used to produce nonhuman transgenic animals. For example, in one embodiment, a host cell of the invention is a fertilized oocyte or an embryonic stem cell into which a sequence encoding a polypeptide of the invention has been introduced. Such host cells can then be used to create non-human transgenic animals in which exogenous sequences encoding a polypeptide of the invention have been introduced into their genome or homologous recombinant animals in which endogenous sequences encoding a polypeptide of the invention have been altered. Such animals are useful for studying the function and/or activity of the polypeptide and for identifying and/or evaluating modulators of polypeptide activity. In addition to particular gene expression and/or polypeptide expression phenotypes, the transgenic animals of the invention can exhibit any of the phenotypes (e.g., processes, disorder symptoms and/or disorders associated with the gene expression). As used herein, a "transgenic animal" is a non-human animal, preferably a mammal, more preferably a rodent such as a rat or mouse, in which one or more of the cells of the animal includes a transgene. Other examples of transgenic animals include non-human primates, sheep, dogs, cows, goats, chickens, amphibians, etc. A transgene is exogenous DNA which is integrated into the genome of a cell from which a transgenic animal develops and which remains in the genome of the mature animal, thereby directing the expression of an encoded gene product in one or more cell types or tissues of the transgenic animal. As used herein, an "homologous recombinant animal" is a non-human animal, preferably a mammal, more preferably a mouse, in which an endogenous gene has been altered by homologous recombination between the endogenous gene and an exogenous DNA molecule introduced into a cell of the animal, e.g., an embryonic cell of the animal, prior to development of the animal.
A transgenic animal of the invention can be created by introducing nucleic acid encoding a polypeptide of the invention or a homologue thereof into the male pronuclei of a fertilized oocyte, for example, by microinjection or retroviral infection, and allowing the oocyte to develop in a pseudopregnant female foster animal. Intronic sequences and polyadenylation signals can also be included in the transgene to increase the efficiency of expression of the transgene. A tissue-specific regulatory sequence(s) can be operably linked to the transgene to direct expression of the polypeptide of the invention to particular cells.
Methods for generating transgenic animals via embryo manipulation and microinjection, particularly animals such as mice, have become conventional in the art and are described, for example, in U.S. Patent Nos. 4,736,866 and 4,870,009, U.S. Patent No.
4,873,191 and in Hogan, Manipulating the Mouse Embryo, (Cold Spring Harbor Laboratory Press, Cold Spring Harbor, N.Y., 1986) and Wakayama etal., (1999), Proc. Natl. Acad. Sci.
USA, 96:14984-14989. Similar methods are used for production of other transgenic animals. A
transgenic founder animal can be identified based upon the presence of the transgene in its genome and/or expression of mRNA encoding the transgene in tissues or cells of the animals. A transgenic founder animal can then be used to breed additional animals carrying the transgene. Moreover, transgenic animals carrying the transgene can further be bred to other transgenic animals carrying other transgenes.
To create an homologous recombinant animal, a vector is prepared which contains at least a portion of a gene encoding a polypeptide of the invention into which a deletion, addition or substitution has been introduced to thereby alter, e.g., functionally disrupt, the gene. In a preferred embodiment, the vector is designed such that, upon homologous recombination, the endogenous gene is functionally disrupted (i.e., no longer encodes a functional protein; also referred to as a "knock out" vector).
Alternatively, the vector can be designed such that, upon homologous recombination, the endogenous gene is mutated or otherwise altered but still encodes functional protein (e.g., the upstream regulatory region can be altered to thereby alter the expression of the endogenous protein).
In the homologous recombination vector, the altered portion of the gene is flanked at its 5' and 3' ends by additional nucleic acid of the gene to allow for homologous recombination to occur between the exogenous gene carried by the vector and an endogenous gene in an embryonic stem cell. The additional flanking nucleic acid sequences are of sufficient length for successful homologous recombination with the endogenous gene. Typically, several kilobases of flanking DNA (both at the 5' and 3' ends) are included in the vector (see, e.g., Thomas and Capecchi (1987) Cell 51:503 for a description of homologous recombination vectors). The vector is introduced into an embryonic stem cell line (e.g., by electroporation) and cells in which the introduced gene has homologously recombined with the endogenous gene are selected (see, e.g., Li et at., 1992, Cell 69:915). The selected cells are then injected into a blastocyst of an animal (e.g., a mouse) to form aggregation chimeras (see, e.g., Bradley in Teratocarcinomas and Embryonic Stein Cells: A Practical Approach, Robertson, ed., IRL, Oxford, 1987, pp. 113-152). A chimeric embryo can then be implanted into a suitable pseudopregnant female foster animal and the embryo brought to term.
Progeny harboring the homologously recombined DNA in their germ cells can be used to breed animals in which all cells of the animal contain the homologously recombined DNA by gennline transmission of the transgene. Methods for constructing homologous recombination vectors and homologous recombinant animals are described further in Bradley, 1991, Current Opinion in Bio/Techizology, 2:823-829, and in PCT
Publication Nos_ WO 90/11354, WO 91/01140, WO 92/0968, and WO 93/04169.
In another embodiment, transgenic non-human animals can be produced which contain selected systems which allow for regulated expression of the transgene. One example of such a system is the cre/loxP recombinase system of bacteriophage Pl. For a description of the cre/loxP recombinase system, see, e.g., Lakso etal., 1992, Proc. Natl.
Acad. Sci. USA, 89:6232-6236. Another example of a recombinase system is the FLP
recombinase system of Saccharonzyces cerevisiae (O'Gorman et at., 1991, Science, 251:1351-1355). If a cre/loxP recombinase system is used to regulate expression of the transgene, animals containing transgenes encoding both the Cre recombinase and a selected protein are required. Such animals can be provided through the construction of "double"
transgenic animals, e.g., by mating two transgenic animals, one containing a transgene encoding a selected protein and the other containing a transgene encoding a recombinase.
Clones of the non-human transgenic animals described herein can also be produced according to the methods described in Wilmut et al., 1997, Nature, 385:810-813, and PCT Publication Nos. WO 97/07668 and WO 97/07669.
5.4 Fusion proteins The present invention further encompasses fusion proteins in which the polypeptides of the invention or fragments thereof, are recombinantly fused or chemically conjugated (including both covalent and non-covalent conjugations) to heterologous polypeptides (i.e., an unrelated polypeptide; or portion thereof, preferably at least 10, at least 20, at least 30, at least 40, at least 50, at least 60, at least 70, at least 80, at least 90 or at least 100 amino acids of the polypeptide) to generate fusion proteins. The fusion does not necessarily need to be direct, but may occur through linker sequences.

In one example, a fusion protein in which a polypeptide of the invention or a fragment thereof can be fused to sequences derived from various types of immunoglobulins.
For example, a polypeptide of the invention can be fused to a constant region (e.g., hinge, CH2, and CH3 domains) of human IgG I or IgM molecule, as described in sections 6.3.1, 6.3.2, and 6.3.4 below, so as to make the fused polypeptides or fragments thereof more soluble and stable in vivo. Such fusion proteins can be used as an immunogen for the production of specific antibodies which recognize the polypeptides of the invention or fragments thereof. In another embodiment, such fusion proteins can be administered to a subject so as to inhibit interactions between a ligand and its receptors in vivo. Such inhibition of the interaction will block or suppress signal transduction which triggers certain cellular responses. One of the examples is described in section 6.10, in which the fusion protein between the extracellular portion of TREM-1 and the constant domain of human IgG1 (TREM-1-hulgG1) was administered to mice before and after the lipopolysaccharide (LPS) injection. The soluble fusion protein protected the mice from septicemia and increased the survival rate presumably by blocking the interactions between the cell surface TREM-1 and its ligand(s), thereby inhibiting inflammatory responses.
In one aspect, the fusion protein comprises a polypeptide of the invention which is fused to a heterologous signal sequence at its N-terminus. For example, the signal sequence naturally found in the polypeptide of the invention can be replaced by a signal sequence which is derived from a heterologous origin. Various signal sequences are commercially available. For example, the secretory sequences of melittin and human placental alkaline phosphatase (Stratagene; La Jolla, CA) are available as eukaryotic heterologous signal sequences. As examples of prokaryotic heterologous signal sequences, the phoA secretory signal (Sambrook et al., supra; and Current Protocols in Molecular Biology, Ausubel et al., eds., John Wiley & Sons, 1992) and the protein A
secretory signal (Pharmacia Biotech; Piscataway, NJ) can be listed. Another example is the gp67 secretory sequence of the baculovirus envelope protein (Current Protocols in Molecular Biology, Ausubel et al., eds., John Wiley & Sons, 1992).
In another embodiment, a polypeptide of the invention can be fused to tag sequences, e.g., a hexa-histidine peptide, such as the tag provided in a pQE
vector (QIAGEN, Inc., 9259 Eton Avenue, Chatsworth, CA, 91311), among others, many of which are commercially available. As described in Gentz et al., 1989, Proc. Natl.
Acad. S'ci. USA, 86:821-824, for instance, hexa-histidine provides for convenient purification of the fusion protein. Other examples of peptide tags are the hemagglutinin "HA" tag, which corresponds to an epitope derived from the influenza hemagglutinin protein (Wilson et al., 1984, ('ell, 37:767) and the "flag" tag (Knappik et al., 1994, Biotechniques, 17(4):754-761).

These tags are especially useful for purification of recombinantly produced golypeptides of the invention.
Fusion proteins can be produced by standard recombinant DNA techniques or by protein synthetic techniques, e.g., by use of a peptide synthesizer. For example, a nucleic acid molecule encoding a fusion protein can be synthesized by conventional techniques including automated DNA synthesizers. Alternatively, PCR
amplification of gene fragments can be carried out using anchor primers which give rise to complementary overhangs between two consecutive gene fragments which can subsequently be annealed and reampli fled to generate a chimeric gene sequence (see, e.g., Current Protocols in Molecular Biology, Ausubel etal., eds., John Wiley & Sons, 1992).
The nucleotide sequence coding for a fusion protein can be inserted into an appropriate expression vector, i.e., a vector which contains the necessary elements for the transcription and translation of the inserted protein-coding sequence. Various host-vector systems and selection systems are available as described in section 5.3.
In a specific embodiment, the expression of a fusion protein is regulated by a constitutive promoter. In another embodiment, the expression of a fusion protein is regulated by an inducible promoter. In accordance with these embodiments, the promoter may be a tissue-specific promoter.
Expression vectors containing inserts of a gene encoding a fusion protein can be identified by three general approaches: (a) nucleic acid hybridization, (b) presence or absence of "marker" gene functions, and (c) expression of inserted sequences.
In the first approach, the presence of a gene encoding a fusion protein in an expression vector can be detected by nucleic acid hybridization using probes comprising sequences that are homologous to an inserted gene encoding the fusion protein. In the second approach, the recombinant vector/host system can be identified and selected based upon the presence or absence of certain "marker" gene functions (e.g., thymidine kinase activity, resistance to antibiotics, transformation phenotype, occlusion body formation in baculovirus, etc.) caused by the insertion of a nucleotide sequence encoding a fusion protein in the vector. For example, if the nucleotide sequence encoding the fusion protein is inserted within the marker gene sequence of the vector, recombinants containing the gene encoding the fusion protein insert can be identified by the absence of the marker gene function.
In the third approach, recombinant expression vectors can be identified by assaying the gene product (i.e., fusion protein) expressed by the recombinant. Such assays can be based, for example, on the physical or functional properties of the fusion protein in in vitro assay systems, e.g., binding with anti-fusion protein antibody.

For long-term, high-yield production of recombinant proteins,'stable expression is preferred. For example, cell lines which stably express the fusion protein may be engineered. Rather than using expression vectors which contain viral origins of replication, host cells can be transformed with DNA controlled by appropriate expression control elements (e.g., promoter, enhancer, sequences, transcription terminators, polyadenylation sites, etc.), and a selectable marker. Following the introduction of the foreign DNA, engineered cells may be allowed to grow for 1-2 days in an enriched medium, and then are switched to a selective medium. The selectable marker in the recombinant plasmid confers resistance to the selection and allows cells to stably integrate the plasmid into their chromosomes and grow to form foci which in turn can be cloned and expanded into cell lines. This method may advantageously be used to engineer cell lines that express the differentially expressed or pathway gene protein. Such engineered cell lines may be particularly useful in screening and evaluation of compounds that affect the endogenous activity of the differentially expressed or pathway gene protein.
Once a fusion protein of the invention has been produced by recombinant expression, it may be purified by any method known in the art for purification of a protein, for example, by chromatography (e.g., ion exchange, affinity, particularly by affinity for the specific antibody, and sizing column chromatography), centrifugation, differential solubility, or by any other standard technique for the purification of proteins.
5.5 Preparation of antibodies Antibodies which specifically recognize a polyp.eptide of the invention or fragments thereof can be used for various diagnostic and therapeutic purposes.
For example, in one specific embodiment, an antibody which is specific for TREM-1 or TREM-2 can be used for various in vitro detection assays, including enzyme-linked immunosorbent assays (ELISA), radioimmunoassays, Western blot, Flow Cytometry analysis, immunohistochemical analysis, and so forth for the detection of TREM-1 or TREM-molecules or fragments thereof in biological samples, such as blood, serum, plasma, urine, saliva, tissues, cells, etc., as well as for in vivo detection of these molecules for diagnostic purposes. For example, anti-TREM-1 antibody can be used in immunohistochemical analysis of pathological tissue specimens to differentiate local and systemic inflammations caused by different types of agents. Since TREM-1 is strongly expressed in the presence of certain bacterial and fungal products, such as LPS, detection of TREM-1 in the inflamed tissue specimens would suggest a bacterial and/or fungal origin of the inflammation.
In another specific embodiment, an anti-TREM-1 or anti-TREM-2 antibody which acts as an antagonist against TREM-1 or TREM-2 (i.e., inhibiting TREM-1 or i TREM-2 activities), respectively, on myeloid cells may be used as a therapeutic agent for reducing systemic and/or local inflammatory responses triggered by causative agents (e.g., bacteria and fungi). Such antibodies can block the binding of ligands to these receptors and, thereby, prevent subsequent signal transduction and inflammatory responses from occurring. Examples of local inflammations include pulmonitis, pleuritis, impetigo, abscesses, sinovitis, arthritis, etc. and systemic inflammations include meningitis, peritonitis, sepsis, etc. Thus, these antibodies are useful in modulating inflammatory responses mediated by TREM-I and/or TREM-2.
In another specific embodiment, anti-TREM-2 antibody may be used as an adjuvant to facilitate the migration of DCs from the periphery to the lymph nodes by cross-linking TREM-2 on DCs and upregulating CCR7 expression by DCs.
Other diagnostic, therapeutic, or prophylactic uses of antibodies specific for the polypeptides of the invention will be further discussed below.
Antibodies specific for the polypeptides of the invention may be generated by any suitable method known in the art. Polyclonal antibodies to an antigen-of-interest can be produced by various procedures well known in the art. For example, an antigen derived from the polypeptide of the invention can be administered to various host animals including, but not limited to, rabbits, mice, rats, etc., to induce the production of antisera containing polyclonal antibodies specific for the antigen. Various adjuvants may be used to increase the immunological response, depending on the host species, and include but are not limited to, Freund's (complete and incomplete) adjuvant, mineral gels such as aluminum hydroxide, surface active substances such as lysolecithin, pluronic polyols, polyanions, peptides, oil emulsions, keyhole limpet hemocyanins, dinitrophenol, and potentially useful adjuvants for humans such as BCG (Bacille Calmette-Guerin) and Corynebacterium parvurn. Such adjuvants are also well known in the art.
Monoclonal antibodies can be prepared using a wide variety of techniques known in the art including the use of hybridoma, recombinant, and phage display technologies, or a combination thereof. For example, monoclonal antibodies can be produced using hybridoma techniques including those known in the art and taught, for example, in Harlow et al., Antibodies: A Laboratory Manual, (Cold Spring Harbor Laboratory Press, 2nd ed. 1988); Hammerling, etal., in: Monoclonal Antibodies and T-Cell Ilybridonzas, pp. 563-681 (Elsevier, N.Y., 1981).
The term "monoclonal antibody" as used herein is not limited to antibodies produced through hybridoma technology. The term "monoclonal antibody"
refers to an antibody that is derived from a single clone, including any eukaryotic, prokaryotic, or phage clone, and not the method by which it is produced.

Methods for producing and screening for specific antibodies using hybridoma technology are routine and well known in the art. In a non-limiting example, mice can be immunized with an antigen of interest or a cell expressing such an antigen.
Once an immune response is detected, e.g., antibodies specific for the antigen are detected in the mouse serum, the mouse spleen is harvested and splenocytes isolated.
The splenocytes are then fused by well known techniques to any suitable myeloma cells.
Hybridomas are selected and cloned by limiting dilution. The hybridoma clones are then assayed by methods known in the art for cells that secrete antibodies capable of binding the antigen. Ascites fluid, which generally contains high levels of antibodies, can be generated by inoculating mice intraperitoneally with positive hybridoma clones.
Antibody fragments which recognize specific epitopes may be generated by known techniques. For example, Fab and F(ab')2 fragments may be produced by proteolytic cleavage of immunoglobulin molecules, using enzymes such as papain (to produce Fab fragments) or pepsin (to produce F(ab')2 fragments). F(ab')2 fragments contain the complete light chain, and the variable region, the CH1 region and the hinge region of the heavy chain.
The antibodies of the invention or fragments thereof can be also produced by any method known in the art for the synthesis of antibodies, in particular, by chemical synthesis or preferably, by recombinant expression techniques.
The nucleotide sequence encoding an antibody may be obtained from any TM
information available to those skilled in the art (i.e., from Genbank, the literature, or by routine cloning). If a clone containing a nucleic acid encoding a particular antibody or an epitope-binding fragment thereof is not available, but the sequence of the antibody molecule or epitope-binding fragment thereof is known, a nucleic acid encoding the immunoglobulin may be chemically synthesized or obtained from a suitable source (e.g., an antibody cDNA library, or a cDNA library generated from, or nucleic acid, preferably poly A+ RNA, isolated from any tissue or cells expressing the antibody, such as hybridoma cells selected to express an antibody) by PCR amplification using synthetic primers hybridizable to the 3' and 5 'ends of the sequence or by cloning using an oligonucleotide probe specific for the particular gene sequence to identify, e.g., a cDNA clone from a cDNA
library that encodes the antibody. Amplified nucleic acids generated by PCR may then be cloned into replicable cloning vectors using any method well known in the art.
Once the nucleotide sequence of the antibody is determined, the nucleotide sequence of the antibody maybe manipulated using methods well known in the art for the manipulation of nucleotide sequences, e.g., recombinant DNA techniques, site directed mutagenesis, PCR, etc. (see, for example, the techniques described in Sambrook et al., supra; and Ausubel et al., eds., 1998, Current Protocols in Molecular Biology;
John Wiley & Sons, NY) to generate antibodies hailing a different amino acid sequence by, for example, introducing amino acid substitutions, deletions, and/or insertions into the epitope-binding domain regions of the . 5 antibodies or any portion of antibodies which may enhance or reduce biological activities of the antibodies.
Recombinant expression of an antibody requires construction of an expression vector containing a nucleotide sequence that encodes the antibody.
Once a nucleotide sequence encoding an antibody molecule or a heavy or light chain of an antibody, or portion thereof has been obtained, the vector for the production of the antibody molecule may be produced by recombinant DNA technology using techniques well known in the art as discussed in the previous sections. Methods which are well known to those skilled in the art can be used to construct expression vectors containing antibody coding sequences and appropriate transcriptional and translational control signals.
These methods include, for example, in vitro recombinant DNA techniques, synthetic techniques, and in vivo genetic recombination. The nucleotide sequence encoding the heavy-chain variable region, light-chain variable region, both the heavy-chain and light-chain variable regions, an epitope-binding fragment of the heavy- and/or light-chain variable region, or one or more complementarity determining regions (CDRs) of an antibody may be cloned into such a vector for expression. Thus-prepared expression vector can be then introduced into appropriate host cells for the expression of the antibody. Accordingly, the invention includes host cells containing a polynucleotide encoding an antibody specific for the polypeptides of the invention or fragments thereof.
The host cell may be co-transfected with two expression vectors of the invention, the first vector encoding a heavy chain derived polypeptide and the second vector encoding a light chain derived polypeptide. The two vectors may contain identical selectable markers which enable equal expression of heavy and light chain polypeptides or different selectable markers to ensure maintenance of both plasmids.
Alternatively, a single vector may be used which encodes, and is capable of expressing, both heavy and light chain polypeptides. In such situations, the light chain should be placed before the heavy chain to avoid an excess of toxic free heavy chain (Proudfoot, Nature, 322:52, 1986;
and Kohler, Proc. Natl. Acad. Sci. USA, 77:2 197, 1980). The coding sequences for the heavy and light chains may comprise cDNA or genomic DNA.
In another embodiment, antibodies can also be generated using various phage display methods known in the art. In phage display methods, functional antibody domains are displayed on the surface of phage particles which carry the polynucleotide -sequences encoding them. In a particular embodiment, such phage can be utilized to display antigen binding domains, such as Fab and Fv or disulfide-bond stabilized Fv, expressed from a repertoire or combinatorial antibody library (e.g., human or murine).
Phage expressing an antigen binding domain that binds the antigen of interest can be selected or identified with antigen, e.g., using labeled antigen or antigen bound or captured to a solid surface or bead. Phage used in these methods are typically filamentous phage, including fd and M13. The antigen binding domains are expressed as a recombinantly fused protein to either the phage gene III or gene VIII protein. Examples of phage display methods that can be used to make the immunoglobulins, or fragments thereof, of the present invention include those disclosed in Brinkman et aL, J. Immunol.
Methods, 182:41-50, 1995; Ames et al., J. Immunol. Methods, 184:177-186, 1995; Kettleborough etal., Eur.
J. ImmunoL, 24:952-958, 1994; Persic et al., Gene, 187:9-18, 1997; Burton et aL, Advances in Immunology, 57:191-280, 1994; PCT application No. PCT/GB91/01134; PCT
publications WO 90/02809; WO 91/10737; WO 92/01047; WO 92/18619; WO 93/11236;
WO 95/15982; WO 95/20401; and U.S. Patent Nos. 5,698,426; 5,223,409;
5,403,484;
5,580,717; 5,427,908; 5,750,753; 5,821,047; 5,571,698; 5,427,908; 5,516,637;
5,780,225;
5,658,727; 5,733,743 and 5,969,108.
As described in the above references, after phage selection, the antibody coding regions from the phage can be isolated and used to generate whole antibodies, including human antibodies, or any other desired fragments, and expressed in any desired host, including mammalian cells, insect cells, plant cells, yeast, and bacteria, e.g., as described in detail below. For example, techniques to recombinantly produce Fab, Fab' and F(ab')2 fragments can also be employed using methods known in the art such as those disclosed in PCT publication WO 92/22324; Mullinax et al., BioTechniques, 12(6):864-869, 1992; and Sawai etal., AJR1, 34:26-34, 1995; and Better et aL. Science, 240:1041-1043, 1988. Examples of techniques which can be used to produce single-chain Evs and antibodies include those described in U.S. Patent Nos. 4,946,778 and 5,258,498; Huston et al., Methods in Enzymology, 203:46-88, 1991; Shu etal., PN AS, 90:7995-7999, 1993; anci-Skerra etal., Science, 240:1038-1040, 1988.
Once an antibody molecule of the invention has been produced by any methods described above, it may then be purified by any method known in the art for purification of an immunoglobulin molecule, for example, by chromatography (e.g., ion exchange, affinity, particularly by affinity for the specific antigen after Protein A or Protein G purification, and sizing column chromatography), centrifugation, differential solubility, or by any other standard techniques for the purification of proteins. Further, the antibodies of the present invention or fragments thereof may be fused to heterologous polypeptide sequences described herein or otherwise known in the art to facilitate purification.
For some uses, including in vivo use of antibodies in humans and in vitro detection assays, it may be preferable to use chimeric, humanized, or human antibodies. A
chimeric antibody is a molecule in which different portions of the antibody are derived from different animal species, such as antibodies having a variable region derived from a murine monoclonal antibody and a constant region derived from a human immunoglobulin.

Methods for producing chimeric antibodies are known in the art. See e.g., Morrison, Science, 229:1202, 1985; Oi etal., BioTechniques, 4:214 1986; Gillies et aL, J. ImmunoL
Methods, 125:191-202, 1989; U.S. Patent Nos. 5,807,715; 4,816,567; and 4,816,397.
Humanized antibodies are antibody molecules from non-human species that bind the desired antigen having one or more complementarity determining regions (CDRs) from the non-human species and framework regions from a human immunoglobulin molecule. Often, framework residues in the human framework regions will be substituted with the corresponding residue from the CDR donor antibody to alter, preferably improve, antigen binding. These framework substitutions are identified by methods well known in the art, e.g., by modeling of the interactions of the CDR and framework residues to identify framework residues important for antigen binding and sequence comparison to identify unusual framework residues at particular positions.
See, e.g., Queen et al., U.S. Patent No. 5,585,089; Rieclunann et al., Nature, 332:323, 1988.
Antibodies can be humanized using a variety of techniques known in the art including, for example, CDR-grafting (EP
239,400; PCT publication WO 91/09967; U.S. Patent Nos. 5,225,539; 5,530,101 and 5,585,089), veneering or resurfacing (EP 592,106; EP 519,596; Padlan, Molecular Immunology, 28(4/5):489-498, 1991; Studnicka et al., Protein Engineering, 7(6):805-814, 1994; Roguska et at., Proc NatL Acad. ScL USA, 91:969-973, 1994), and chain shuffling (U.S. Patent No. 5,565,332).
Completely human antibodies are particularly desirable for therapeutic treatment of human patients. Human antibodies can be made by a variety of methods known in the art including phage display methods described above using antibody libraries derived from human immunoglobulin sequences. See U.S. Patent Nos. 4,444,887 and 4,716,111; and PCT publications WO 98/46645; WO 98/50433; WO 98/24893; WO
98/16654; WO 96/34096; WO 96/33735; and WO 91/10741.

Human antibodies can also be produced using transgenic mice which are incapable of expressing functional endogenous immunoglobulins, but which can express human immunoglobulin genes. For an overview of this technology for producing human antibodies, see Lonberg and Huszar, Int. Rev. 1177171111101., 13:65-93, 1995.
For a detailed discussion of this technology for producing human antibodies and human monoclonal antibodies and protocols for producing such antibodies, see, e.g., PCT
publications WO
98/24893; WO 92/01047; WO 96/34096; WO 96/33735; European Patent No. 0 598 877;
U.S. Patent Nos. 5,413,923; 5,625,126; 5,633,425; 5,569,825; 5,661,016;
5,545,806;
5,814,318; 5,885,793; 5,916,771; and 5,939,598.
In addition, companies such as Abgenix, Inc. (Freemont, CA), Medarex (NJ) and Genpharrn (San Jose, CA) can be engaged to provide human antibodies directed against a selected antigen using technology similar to that described above.
Completely human antibodies which recognize a selected epitope can be generated using a technique referred to as "guided selection." In this approach a selected non-human monoclonal antibody, e.g., a mouse antibody, is used to guide the selection of a completely human antibody recognizing the same epitope. (Jespers et al., Rio/technology, 12:899-903, 1988).
Antibodies fused or conjugated to heterologous polypeptides may be used in in vitro immunoassays and in purification methods (e.g., affinity chromatography) well known in the art. See e.g., PCT publication Number WO 93/21232; EP 439,095;
Naramura etal., Immunol. Lett., 39:91-99, 1994; U.S. Patent 5,474,981; Gillies etal., PNAS, 89:1428-1432, 1992; and Fell et al., J. Immunol., 146:2446-2452, 1991.
The present invention also encompasses antibodies conjugated to a diagnostic or therapeutic agent. The antibodies can be used diagnostically to, for example, monitor the development or progression of a disease, disorder or infection as part of a clinical testing procedure to, e.g., determine the efficacy of a given treatment regimen.
Detection can be facilitated by coupling the antibody to a detectable substance. Examples of detectable substances include various enzymes, prosthetic groups, fluorescent materials, luminescent materials, bioluminescent materials, radioactive materials, positron emitting metals, and nonradioactive paramagnetic metal ions. The detectable substance may be coupled or conjugated either directly to the antibody or indirectly, through an intermediate (such as, for example, a linker known in the art) using techniques known in the art. See, for example, U.S. Patent No. 4,741,900 for metal ions which can be conjugated to antibodies for use as diagnostics according to the present invention. Examples of suitable enzymes include horseradish peroxidase, alkaline phosphatase, beta-galactosidase, or acetylcholinesterase; examples of suitable prosthetic group complexes include-streptavidin/biotin and avidin/biotin; examples of suitable fluorescent materials include umbelliferone, fluorescein, fluorescein isothiocyanate, rhodamine, dichlorotriazinylamine fluorescein, dansyl chloride or phycoerythrin; an example of a luminescent material includes luminol; examples of bioluminescent materials include luciferase, luciferin, and aequorin; and examples of suitable radioactive material include 1251, '3'1, "In or 99"Tc..
An antibody may be conjugated to a therapeutic moiety such as a cytotoxin (e.g., a cytostatic or cytocidal agent), a therapeutic agent or a radioactive element (e.g., alpha-emitters, gamma-emitters, etc.). Cytotoxins or cytotoxic agents include any agent that is detrimental to cells. Examples include paclitaxol, cytochalasin B, gramicidin D, ethidium bromide, emetine, mitomycin, etoposide, tenoposide, vincristine, vinblastine, colchicin, doxorubicin, daunorubicin, dihydroxy anthracin dione, mitoxantrone, mithramycin, actinomycin D, 1-dehydrotestosterone, glucocorticoids, procaine, tetracaine, lidocaine, propranolol, and puromycin and analogs or homologues thereof.
Therapeutic .
agents include, but are not limited to, anti-inflammatory agents (e.g., anti-TNF-a antibody, e.g., REMICADE (infliximab) (Centocor, PA), IL-1 receptor antagonist, anti-MIF
antibody, anti-HMG-1 antibody, and methotrexate), antimetabolites (e.g., methotrexate, 6-mercaptopurine, 6-thioguanine, cytarabine, 5-fluorouraci1 decarbazine), alkylating agents (e.g., mechlorethamine, thioepa chlorambucil, melphalan, carmustine (BSNU) and lomustine (CCNU), cyclothosphamide, busulfan, dibromomannitol, streptozotocin, mitomycin C, and cisdichlorodiamine platinum (11) (DDP) cisplatin), anthracyclines (e.g., daunorubicin (formerly daunomycin) and doxorubicin), antibiotics (e.g., dactinomycin (formerly actinomycin), bleomycin, mithramycin, and anthramycin (AMC)), and anti-mitotic agents (e.g., vincristine and vinblastine).
Further, an antibody may be conjugated to a therapeutic agent or drug moiety that modifies a given biological response. Therapeutic agents or drug moieties are not to be construed as limited to classical chemical therapeutic agents. For example, the drug moiety may be a protein or polypeptide possessing a desired biological activity.
In one specific embodiment, an antibody specific for TREM-1 (anti-TREM-1 antibody) which is coupled with a certain cytotoxin or a drug can be used in a therapy to target leukemia of myeloid origin expressing TREM-1. The anti-TREM-I
administered to a subject will deliver the cytotoxin or drug to tumor cells expressing TREM-1, thereby killing specifically the tumor cells.
In another embodiment, an antibody specific for TREM-2 (anti-TREM-2 antibody) can be used for an efficient presentation of an antigen of interest by DCs to T
cells. Since TREM-2 is expressed only or at least predominantly in DCs in peripheral tissues (e.g., skin and mucosa), an anti-TREM-2 antibody coupled with an antigen of interest can effectively and efficiently deliver the antigen to peripheral DCs, which subsequently process and present the antigen to the T cells at lymph nodes. An antigen of interest can be chemically or genetically conjugated to an anti-TREM-2 antibody or, alternatively, the anti-TREM-2 antibody can be genetically engineered to become bispecific for TREM-2 on one arm and for the antigen of interest on the other. This approach may have a great utility in preparing various vaccines.
Techniques for conjugating such therapeutic moieties to antibodies are well known; see, e.g., Amon et al., "Monoclonal Antibodies For Immunotargeting Of Drugs In Cancer Therapy", in Monoclonal Antibodies And Cancer Therapy, Reisfeld et al.
(eds.), 1985, pp. 243-56, Alan R. Liss, Inc.; Hellstrom etal., "Antibodies For Drug Delivery", in Controlled Drug Delivery (2nd Ed.), Robinson etal. (eds.), 1987, pp. 623-53, Marcel Dekker, Inc.; Thorpe, "Antibody Carriers Of Cytotoxic Agents In Cancer Therapy: A
Review", in Monoclonal Antibodies '84: Biological And Clinical Applications, Pinchera et al. (eds.), 1985, pp. 475-506; "Analysis, Results, And Future Prospective Of The Therapeutic Use Of Radiolabeled Antibody In Cancer Therapy", in Monoclonal Antibodies For Cancer Detection And Therapy, Baldwin etal. (eds.),1985, pp. 303-16, Academic Press; and Thorpe etal., Innnurzol. Rev., 62:119-58, 1982.
Alternatively, an antibody can be conjugated to a second antibody to form an antibody heteroconiugate as described by Segal in U.S. Patent No. 4,676,980, Antibodies may also be attached to solid supports, which are particularly useful for immunoassays or purification of the target antigen. Such solid supports include, but are not limited to, glass, cellulose, polyacrylamide, nylon, polystyrene, polyvinyl chloride or polypropylene.
5.6 Pharmaceutical compositions The nucleic acid molecules, polypeptides, and antibodies (also referred to herein as "active compounds") of the invention can be incorporated into pharmaceutical compositions suitable for administration. Such compositions typically comprise the nucleic acid molecule, protein, or antibody and a pharmaceutically acceptable carrier.
As used herein the language "pharmaceutically acceptable carrier" is intended to include any and all solvents, dispersion media, coatings, antibacterial and antifungal agents, isotonic and absorption delaying agents, and the like, compatible with pharmaceutical administration.
The use of such media and agents for pharmaceutically active substances is well known in the art. Except insofar as any conventional media or agent is incompatible with the active compound, use thereof in the compositions is contemplated. Supplementary active compounds can also be incorporated into the compositions.
The invention includes methods for preparing pharmaceutical compositions for modulating the expression or activity of a polypeptide or nucleic acid of the invention.
Such methods comprise formulating a pharmaceutically acceptable carrier with an agent which modulates expression or activity of a polypeptide or nucleic acid of the invention.
Such compositions can further include additional active agents. Thus, the invention further includes methods for preparing a pharmaceutical composition by formulating a pharmaceutically acceptable carrier with an agent which modulates expression or activity of a polypeptide or nucleic acid of the invention and one or more additional active compounds.
A pharmaceutical composition of the invention is formulated to be compatible with its intended route of administration. Examples of routes of administration include parenteral, e.g., intravenous, intradermal, subcutaneous, transdermal (topical), transmucosal, intra-articular, intraperitoneal, and intrapleural, as well as oral, inhalation, and rectal administration. Solutions or suspensions used for parenteral, intradermal, or subcutaneous application can include the following components: a sterile diluent such as water for injection, saline solution, fixed oils, polyethylene glycols, glycerine, propylene glycol or other synthetic solvents; antibacterial agents such as benzyl alcohol or methyl parabens; antioxidants such as ascorbic acid or sodium bisulfite; chelating agents such as ethylenediaminetetraacetic acid; buffers such as acetates, citrates or phosphates and agents for the adjustment of tonicity such as sodium chloride or dextrose. pH can be adjusted with acids or bases, such as hydrochloric acid or sodium hydroxide. The parenteral preparation can be enclosed in ampoules, disposable syringes or multiple dose vials made of glass or plastic.
Pharmaceutical compositions suitable for injectable use include sterile aqueous solutions (where water soluble) or dispersions and sterile powders for the extemporaneous preparation of sterile injectable solutions or dispersions. For intravenous administration, suitable carriers include physiological saline, bacteriostatic water, Cremophor ELTM (BASF; Parsippany, NJ) or phosphate buffered saline (PBS). In all cases, the composition must be sterile and should be fluid to the extent that easy injectability with a syringe exists. It must be stable under the conditions of manufacture and storage and must be preserved against the contaminating action of microorganisms such as bacteria and fungi.
The carrier can be a solvent or dispersion medium containing, for example, water, ethanol, polyol (for example, glycerol, propylene glycol, and liquid polyetheylene glycol, and the like), and suitable mixtures thereof. The proper fluidity can be maintained, for example, by the use of a coating such as lecithin, by the maintenance of the required particle size in the case of dispersion and by the use of surfactants. Prevention of the action of microorganisms can be achieved by various antibacterial and antifungal agents, for example, parabens, chlorobutanol, phenol, ascorbic acid, thimerosal, and the like. In many cases, it will be preferable to include isotonic agents, for example, sugars, polyalcohols such as mannitol, sorbitol, sodium chloride in the composition. Prolonged absorption of the injectable compositions can be brought about by including in the composition an agent which delays absorption, for example, aluminum monostearate and gelatin.
Sterile injectable solutions can be prepared by incorporating the active compound (e.g., a polypeptide or antibody) in the required amount in an appropriate solvent with one or a combination of ingredients enumerated above, as required, followed by filtered sterilization. Generally, dispersions are prepared by incorporating the active compound into a sterile vehicle which contains a basic dispersion medium and the required other ingredients from those enumerated above. In the case of sterile powders for the preparation of sterile injectable solutions, the preferred methods of preparation are vacuum drying and freeze-drying which yields a powder of the active ingredient plus any additional desired ingredient from a previously sterile-filtered solution thereof.
Oral compositions generally include an inert diluent or an edible carrier.
They can be enclosed in gelatin capsules or compressed into tablets. For the purpose of oral therapeutic administration, the active compound can be incorporated with excipients and used in the form of tablets, troches, or capsules. Oral compositions can also be prepared using a fluid carrier for use as a mouthwash, wherein the compound in the fluid carrier is applied orally and swished and expectorated or swallowed.
Pharmaceutically compatible binding agents, and/or adjuvant materials can be included as part of the composition. The tablets, pills, capsules, troches and the like can contain any of the following ingredients, or compounds of a similar nature: a binder such as microcrystalline cellulose, gum tragacanth or gelatin; an excipient such as starch or lactose, TM
a disintegrating agent such as alginic acid, Primogei. or corn starch; a lubricant such as magnesium stearate or SteroteTMs; a glidant such as colloidal silicon dioxide;
a sweetening agent such as sucrose or saccharin; or a flavoring agent such as peppermint, methyl salicylate, or orange flavoring.
For administration by inhalation, the compounds are delivered in the form of an aerosol spray from a pressurized container or dispenser which contains a suitable propellant, e.g., a gas such as carbon dioxide, or a nebulizer.
Systemic administration can also be by transmucosal or transdermal means.
For transmucosal or transdermal administration, penetrants appropriate to the barrier to be permeated are used in the formulation. Such penetrants are generally known in the art, and include, for example, for transmucosal administration, detergents, bile salts, and fusidic acid derivatives. Transmucosal administration can be accomplished through the use of nasal sprays or suppositories_ For transdennal administration, the active compounds are formulated into ointments, salves, gels, or creams as generally known in the art.
The compounds can also be prepared in the form of suppositories (e.g., with conventional suppository bases such as cocoa butter and other glycerides) or retention enemas for rectal delivery.
In one embodiment, the active compounds are prepared with carriers that will protect the compound against rapid elimination from the body, such as a controlled release formulation, including implants and microencapsulated delivery systems.
Biodegradable, biocompatible polymers can be used, such as ethylene vinyl acetate, polyanhydrides, polyglycolic acid, collagen, polyorthoesters, and polylactic acid. Methods for preparation of such formulations will be apparent to those skilled in the art. The materials can also be obtained commercially from Alza Corporation and Nova Pharmaceuticals, Inc. Liposomal suspensions (including liposomes targeted to infected cells with monoclonal antibodies to viral antigens) can also be used as pharmaceutically acceptable carriers. These can be prepared according to methods known to those skilled in the art, for example, as described in U.S. Patent No. 4,522,811.
It is especially advantageous to formulate oral or parenteral compositions in dosage unit form for ease of administration and uniformity of dosage. Dosage unit form as used herein refers to physically discrete units suited as unitary dosages for the subject to be treated; each unit containing a predetermined quantity of active compound calculated to produce the desired therapeutic effect in association with the required pharmaceutical carrier. The specification for the dosage unit forms of the invention are dictated by and directly dependent on the unique characteristics of the active compound and the particular therapeutic effect to be achieved, and the limitations inherent in the art of compounding such an active compound for the treatment of individuals.
For antibodies, the preferred dosage is 0.1 mg/kg to 100 mg/kg of body weight (generally 10 mg/kg to 20 mg/kg). If the antibody is to act in the brain, a dosage of 50 mg/kg to 100 mg/kg is usually appropriate_ Generally, partially human antibodies and fully human antibodies have a longer half-life within the human body than other antibodies.
Accordingly, lower dosages and less frequent administration is often possible.
Modifications such as lipidation can be used to stabilize antibodies and to enhance uptake and tissue penetration (e.g., into the brain). A method for lipidation of antibodies is described by Cruikshank et al. (1997, J. Acquired Immune Deficiency Syndromes and Human Retrovirology, 14:193).

Antibodies or antibodies conjugated to therapeutic moieties can be administered to an individual alone or in combination with cytotoxic factor(s), chemotherapeutic drug(s), anti-inflammatory agents, and/or cytokine(s). If the latter, - preferably, the antibodies are administered first and the cytotoxic factor(s), chemotherapeutic drug(s), anti-inflammatory agents, and/or cytokine(s) are administered thereafter within 24 hours. The antibodies and cytotoxic factor(s), chemotherapeutic drug(s) and/or cytokine(s) can be administered by multiple cycles depending upon the clinical response of the patient. Further, the antibodies and cytotoxic factor(s), chemotherapeutic drug(s) and/or cytokine(s) can be administered by the same or separate routes, for example, by intravenous, intranasal or intramuscular administration. Cytotoxic factors include, but are not limited to, TNF-a, TNF-0, IL -1, IFN-y and IL-2.
Chemotherapeutic drugs include, but are not limited to, 5-fluorouracil (5F1J), vinblastine, actinomycin D, etoposide, cisplatin, methotrexate and doxorubicin. Cytokines include, but are not limited to, IL-2, IL-3, IL-4, IL-5, IL-6, IL-7, IL-8, IL-9, IL-10 and IL-12.
As defined herein, a therapeutically effective amount of protein or polypeptide (i.e., an effective dosage) ranges from about 0.001 to 30 mg/kg body weight, preferably about 0.01 to 25 mg/kg body weight, more preferably about 0.1 to 20 mg/kg body weight, and even more preferably about 1 to 10 mg/kg, 2 to 9 mg/kg, 3 to 8 mg/kg, 4 to 7 mg/kg, or 5 to 6 mg/kg body weight.
The skilled artisan will appreciate that certain factors may influence the dosage required to effectively treat a subject, including but not limited to the severity of the disease or disorder, previous treatments, the general health and/or age of the subject, and other diseases present. Moreover, treatment of a subject with a therapeutically effective amount of a protein, polypeptide, or antibody can include a single treatment or, preferably, can include a series of treatments. In a preferred example, a subject is treated with antibody, protein, or polypeptide in the range of between about 0.1 to 20 mg/kg body weight, one time per week for between about 1 to 10 weeks, preferably between 2 to 8 weeks, more preferably between about 3 to 7 weeks, and even more preferably for about 4, 5, or 6 weeks. It will also be appreciated that the effective dosage of antibody, protein, or polypeptide used for treatment may increase or decrease over the course of a particular treatment. Changes in dosage may result and become apparent from the results of diagnostic assays as described herein.
The present invention encompasses agents which modulate expression or activity. An agent may, for example, be a small molecule. For example, such small molecules include, but arc not limited to, peptides, peptidomimetics, amino acids, amino acid analogs, polynucleotides, polynucleotide analogs, nucleotides, nucleotide analogs, organic or inorganic compounds (i.e,. including heteroorganic and organometallic compounds) having a molecular weight less than about 10,000 grams per mole, organic or inorganic compounds having a molecular weight less than about 5,000 grams per mole, organic or inorganic compounds having a molecular weight less than about 1,000 grams per mole, organic or inorganic compounds having a molecular weight less than about grams per mole, and salts, esters, and other pharmaceutically acceptable forms of such compounds.
It is understood that appropriate doses of small molecule agents depends upon a number of factors within the ken of the ordinarily skilled physician, veterinarian, or researcher. The dose(s) of the small molecule will vary, for example, depending upon the identity, size, and condition of the subject or sample being treated, further depending upon the route by which the composition is to be administered, if applicable, and the effect which the practitioner desires the small molecule to have upon the nucleic acid or polypeptide of the invention. Exemplary doses include milligram or microgram amounts of the small molecule per kilogram of subject or sample weight (e.g., about 1 microgram per kilogram to about 500 milligrams per kilogram, about 100 micrograms per kilogram to about milligrams per kilogram, or about 1 microgram per kilogram to about 50 micrograms per kilogram. It is furthermore understood that appropriate doses of a small molecule depend upon the potency of the small molecule with respect to the expression or activity to be 2() modulated. Such appropriate doses may be determined using the assays described herein.
When one or more of these small molecules is to be administered to an animal (e.g., a human) in order to modulate expression or activity of a polypeptide or nucleic acid of the invention, a physician, veterinarian, or researcher may, for example, prescribe a relatively low dose at first, subsequently increasing the dose until an appropriate response is obtained.
In addition, it is understood that the specific dose level for any particular animal subject will depend upon a variety of factors including the activity of the specific compound employed, the age, body weight, general health, gender, and diet of the subject, the time of administration, the route of administration, the rate of excretion, any drug combination, and the degree of expression or activity to be modulated.
The nucleic acid molecules of the invention can be inserted into vectors and used as gene therapy vectors. Gene therapy vectors can be delivered to a subject by, for example, intravenous injection, local administration (U.S. Patent 5,328,470) or by stereotactic injection (see, e.g., Chen et al., 1994, Proc. Natl. Acad. Sci.
USA, 91:3054-3057). The pharmaceutical preparation of the gene therapy vector can include the gene therapy vector in an acceptable diluent, or can comprise a slow release matrix in which the gene delivery vehicle is imbedded. Alternatively, where the complete gene delivery vector can be produced intact from recombinant cells, e.g., retroviral vectors the pharmaceutical preparation can include one or more cells which produce the gene delivery system. With regard to gene therapy, see further discussion in section 5.8.3.
The pharmaceutical compositions can be included in a container, pack, or dispenser together with instructions for administration.
5.7 Utility and Methods of the Invention The nucleic acid molecules, proteins, protein homologues, derivatives, variants, and antibodies described herein can be used in one or more of the following methods: a) screening assays; b) detection assays (e.g., chromosomal mapping, tissue typing); c) predictive medicine (e.g., diagnostic assays and prognostic assays); and d) methods of treatment (e.g., therapeutic and prophylactic). For example, polypeptides of the invention can be used to (i) modulate cellular proliferation; (ii) modulate cellular differentiation; and/or (iii) modulate cellular adhesion. The isolated nucleic acid molecules of the invention can be used to express proteins (e.g., via a recombinant expression vector in a host cell in gene therapy applications), to detect mRNA (e.g., in a biological sample) or a genetic lesion, and to modulate activity of a polypeptide of the invention. In addition, the polypeptides of the invention can be used to screen drugs or compounds which modulate activity or expression of a polypeptide of the invention as well as to treat disorders characterized by insufficient or excessive production of a protein of the invention or production of a form of a protein of the invention which has decreased or aberrant activity compared to the wild type protein_ In addition, the antibodies of the invention can be used to detect and isolate a protein of the invention and modulate activity of a protein of the invention.
This invention further pertains to novel agents identified by the above-described screening assays and uses thereof for treatments as described herein.
5.7.1 Screening assays The invention provides a method for identifying (or screening) modulators, i.e., candidate or test compounds or agents (e.g., peptides, peptidomimetics, small molecules or other drugs) which bind to a polypeptide of the invention or have a stimulatory or inhibitory effect on, for example, expression or activity of a polypeptide of the invention.
In one embodiment, the invention provides assays for screening candidate or test compounds which bind to or modulate the activity of the membrane-bound form of a polypeptide of the invention or biologically active portion thereof. The test compounds of the present invention can be obtained using any of the numerous approaches in combinatorial library methods known in the art, including: biological libraries; spatially addressable parallel solid phase or solution phase libraries; synthetic library methods requiring deconvolution; the "one-bead one-compound" library method; and synthetic library methods using affinity chromatography selection. The biological library approach is limited to peptide libraries, while the other four approaches are applicable to peptide, non-peptide oligomer or small molecule libraries of compounds (Lam (1997) Anticancer Drug Des. 12:145).
Examples of methods for the synthesis of molecular libraries can be found in the art, for example in: DeWitt et al. (1993) Proc. Natl. Acad. Sci. USA
90:6909; Erb et al.
1() (1994) Proc. Natl. Acad. Sci. USA 91:11422; Zuckermann et al. (1994). J.
Med. Chem.
37:2678; Cho et al. (1993) Science 261:1303; Carrell et al. (1994) Angew.
Chem. Int. Ed.
Engl. 33:2059; Carell et al. (1994) Angew. Chem. Int. Ed. EngL 33:2061; and Gallop et al.
(1994)1. Med. Chem. 37:1233.
Libraries of compounds may be presented in solution (e.g., Houghten (1992) Bio/Techniques 13:412-421), or on beads (Lam (1991) Nature 354:82-84), chips (Fodor (1993) Nature 364:555-556), bacteria (U.S. Patent No. 5,223,409), spores (Patent NOS.
5,571,698; 5,403,484; and 5,223,409), plasmids (Cull et al. (1992) Proc. Natl.
Acad. Sci.
USA 89:1865-1869) or phage (Scott and Smith (1990) Science 249:386-390; Devlin (1990) Science 249:404-406; Cwirla et al. (1990) Proc. Natl. Acad. Sci. USA 87:6378-6382; and Felici (1991)J. Mol. Biol. 222:301-310).
In one embodiment, an assay is a cell-based assay in which a cell which expresses a membrane-bound form of a polypeptide of the invention, or a biologically active portion thereof, on the cell surface is contacted with a test compound and the ability of the test compound to bind to the polypeptide determined. The cell, for example, can be a yeast cell or a cell of mammalian origin. Determ. ining the ability of the test compound to bind to the polypeptide can be accomplished, for example, by coupling the test compound with a radioisotope or enzymatic label such that binding of the test compound to the polypeptide or biologically active portion thereof can be determined by detecting the labeled compound in a complex. For example, test compounds can be labeled with 1251, 35s, 14C, or 3H, either directly or indirectly, and the radioisotope detected by direct counting of radioemmission or by scintillation counting. Alternatively, test compounds can be enzymatically labeled with, for example, horseradish peroxidase, alkaline phosphatase, or luciferase, and the enzymatic label detected by determination of conversion of an appropriate substrate to product. In a preferred embodiment, the assay comprises contacting a cell which expresses a membrane-bound form of a polypeptide of the invention, or a biologically active portion thereof, on the cell surface with a known compound which binds the polypeptide to form an assay mixture, contacting the assay mixture with a test compound, and determining the ability of the test compound to interact with the polypeptide, wherein determining the ability of the test compound to interact with the polypeptide comprises determining the ability of the test compound to preferentially bind to the polypeptide or a biologically active portion thereof as compared to the known compound.
In another embodiment, an assay is a cell-based assay comprising contacting a cell expressing a membrane-bound form of a polypeptide of the invention, or a biologically active portion thereof, on the cell surface with a test compound and determining the ability of the test compound to modulate (e.g., stimulate or inhibit) the activity of the polypeptide or biologically active portion thereof.
Determining the ability of the test compound to modulate the activity of the polypeptide or a biologically active portion thereof can be accomplished, for example, by determining the ability of the polypeptide protein to bind to or interact with a target molecule.
Determining the ability of a polypeptide of the invention to bind to or interact with a target molecule can be accomplished by one of the methods described above for determining direct binding. As used herein, a "target molecule" is a molecule with which a selected polypeptide (e.g., a polypeptide of the invention) binds or interacts with in nature, for example, a molecule on the surface of a cell which expresses the selected protein, a molecule on the surface of a second cell, a molecule in the extracellular milieu, a molecule associated with the internal surface of a cell membrane or a cytoplasmic molecule.
A target molecule can be a polypeptide of the invention or some other polypeptide or protein. For example, a target molecule can be a component of a signal transduction pathway which facilitates transduction of an extracellular signal (e.g., a signal generated by binding of a compound to a polypeptide of the invention) through the cell membrane and into the cell or a second intercellular protein which has catalytic activity or a protein which facilitates the association of downstream signaling molecules with a polypeptide of the invention. Determining the ability of a polypeptide of the invention to bind to or interact with a target molecule can be accomplished by determining the activity of the target molecule. For example, the activity of the target molecule can be determined by detecting induction of a cellular second messenger of the target (e.g., intracellular Ca2+, protein tyrosine phosphorylation, phospholipase phosphorylation, etc.), detecting catalytic/enzymatic activity of the target on an appropriate substrate, detecting the induction of a reporter gene (e.g., a regulatory element that is responsive to a polypeptide of the invention operably linked to a nucleic acid encoding a detectable marker, e.g., luciferase), or detecting a cellular response, for example, cellular differentiation, or cell proliferation.

In yet another embodiment, an assay of the present invention is-a cell-free assay comprising contacting a polypeptide of the invention or biologically active portion thereof with a test compound and determining the ability of the test compound to bind to the polypeptide or biologically active portion thereof. Binding of the test compound to the polypeptide can be determined either directly or indirectly as described above. In a preferred embodiment, the assay includes contacting the polypeptide of the invention or biologically active portion thereof with a known compound which binds the polypeptide to form an assay mixture, contacting the assay mixture with a test compound, and determining the ability of the test compound to interact with the polypeptide, wherein determining the ability of the test compound to interact with the polypeptide comprises determining the ability of the test compound to preferentially bind to the polypeptide or biologically active portion thereof as compared to the known compound.
In another embodiment, an assay is a cell-free assay comprising contacting a polypeptide of the invention or biologically active portion thereof with a test compound and determining the ability of the test compound to modulate (e.g., stimulate or inhibit) the activity of the polypeptide or biologically active portion thereof.
Determining the ability of the test compound to modulate the activity of the polypeptide can be accomplished, for example, by determining the ability of the polypeptide to bind to a target molecule by one of the methods described above for determining direct binding. In an alternative embodiment, determining the ability of the test compound to modulate the activity of the polypeptide can be accomplished by determining the ability of the polypeptide of the invention to further modulate the target molecule. For example, the catalytic/enzymatic activity of the target molecule on an appropriate substrate can be determined as previously described.
In yet another embodiment, the cell-free assay comprises contacting a polypeptide of the invention or biologically active portion thereof with a known compound which binds the polypeptide to form an assay mixture, contacting the assay mixture with a test compound, and determining the ability of the test compound to interact with the polypeptide, wherein determining the ability of the test compound to interact with the polypeptide comprises determining the ability of the polypeptidc to preferentially bind to or modulate the activity of a target molecule.
The cell-free assays of the present invention are amenable to use of both a soluble form or the membrane-bound form of a polypeptide of the invention. In the case of cell-free assays comprising the membrane-bound form of the polypeptide, it may be desirable to utilize a solubilizing agent such that the membrane-bound form of the polypeptide is maintained in solution. Examples of such solubilizing agents include non-ionic detergents such as n-octylglucoside, n-dodecylglucoside, n-octylmaltoside TM TM
octanoyl-N-methylglucamide, decanoyl-N-methylglucamide, Tritciri.X-100, Triton X-114, TM
Thesit Isotridecypoly(ethylene glycol ether)õ, 3-[(3-cholamidopropyl)dimethylamminio]-1-propane sulfonate (CHAPS), 34(3-cholamidopropyl)dimethylamminio]-2-hydroxy-1-propane sulfonate (CHAPS0), or N-dodecy1=N,N-dimethy1-3-ammonio-1-propane sulfonate.
In more than one embodiment of the above assay methods of the present invention, it may be desirable to immobilize either the polypeptide of the invention or its ' target molecule to facilitate separation of complexed from uncomplexed forms of one or both of the proteins, as well as to accommodate automation of the assay.
Binding of a test compound to the polypeptide, or interaction of the polypeptide with a target molecule in the presence and absence of a candidate compound, can be accomplished in any vessel suitable for containing the reactants. Examples of such vessels include microtitre plates, test tubes, and micro-centrifuge tubes. In one embodiment, a fusion protein can be provided which adds a domain that allows one or both of the proteins to be bound to a matrix.
For example, glutathione-S-transferase fusion proteins or glutathione-S-transferase fusion proteins can be adsorbed onto glutathione sepharose beads (Sigma Chemical; St. Louis, MO) or glutathione derivatized microtitre plates, which are then combined with the test compound or the test - compound and either the non-adsorbed target protein or a polypeptide of the invention, and the mixture incubated under conditions conducive to complex formation (e.g., at physiological conditions for salt and pH). Following incubation, the beads or microtitre plate wells are washed to remove any unbound components and complex formation is measured either directly or indirectly, for example, as described above.
Alternatively, the complexes can be dissociated from the matrix, and the level of binding or activity of the polypeptide of the invention can be determined using standard techniques.
Other techniques for immobilizing proteins on matrices can also be used in the screening assays of the invention. For example, either the polypeptide of the invention or its target molecule can be immobilized utilizing conjugation of biotin and streptavidin.
Biotinylated polypeptide of the invention or target molecules can be prepared from biotin-NHS (N-hydroxy-succinimide) using techniques well known in the art (e.g., biotinylation kit, Pierce Chemicals; Rockford, IL), and immobilized in the wells of streptavidin-coated 96 well plates (Pierce Chemical). Alternatively, antibodies reactive with the polypeptide of the invention or target molecules but which do not interfere with binding of the polypeptide of the invention to its target molecule can be derivatized to the wells of the plate, and unbound target or polypeptide of the invention trapped in the wells by antibody conjugation. Methods for detecting such complexes, in addition to those described above for the GST-immobilized complexes, include immunodetection of complexes using antibodies reactive with the polypeptide of the invention or target molecule, as well as enzyme-linked assays which rely on detecting an enzymatic activity associated with the polypeptide of the invention or target molecule.
In another embodiment, modulators of expression of a polypeptide of the invention are identified in a method in which a cell is contacted with a candidate compound and the expression of the selected mRNA or protein (i.e., the mRNA or protein corresponding to a polypeptide or nucleic acid of the invention) in the cell is determined.
The level of expression of the selected mRNA or protein in the presence of the candidate compound is compared to the level of expression of the selected mRNA or protein in the absence of the candidate compound. The candidate compound can then be identified as a modulator of expression of the polypeptide of the invention based on this comparison. For example, when expression of the selected mRNA or protein is greater (statistically significantly greater) in the presence of the candidate compound than in its absence, the candidate compound is identified as a stimulator of the selected mRNA or protein expression. Alternatively, when expression of the selected mRNA or protein is less (statistically significantly less) in the presence of the candidate compound than in its absence, the candidate compound is identified as an inhibitor of the selected mRNA or protein expression. The level of the selected mRNA or protein expression in the cells can be determined by methods described herein.
in yet another aspect of the invention, a polypeptide of the inventions can be used as "bait proteins" in a two-hybrid assay or three hybrid assay (see, e.g., U.S. Patent No.
5,283,317; Zervos et. al. (1993) Cell 72:223-232; Madura et al. (1993) 1 Biol.
Chem.
268:12046-12054; Bartel et al. (1993) Bio/Techniques 14:920-924; lwabuchi et al. (1993) Oncogene 8:1693-1696; and PCT Publication No. WO 94/10300), to identify other proteins, which bind to or interact with the polypeptide of the invention and modulate activity of the polypeptide of the invention. Such binding proteins are also likely to be involved in the propagation of signals by the polypeptide of the inventions as, for example, upstream or downstream elements of a signaling pathway involving the polypeptide of the invention.
This invention further pertains to novel agents identified by the above-described screening assays and uses thereof for treatments as described herein.
5.7.2 Detection assays Portions or fragments of the cDNA sequences identified herein (and the corresponding complete gene sequences) can be used in numerous ways as polynucleotide reagents. For example, these sequences can be used to: (i) map their respective genes on a chromosome and, thus, locate gene regions associated with genetic disease;
(ii) identify an individual from a minute biological sample (tissue typing); and (iii) aid in forensic identification of a biological sample. These applications are described in the subsections below.
A. Chromosome mapping Once the sequence (or a portion of the sequence) of a gene has been isolated, this sequence can be used to map the location of the gene on a chromosome.
Accordingly, nucleic acid molecules described herein or fragments thereof, can be used to map the location of the corresponding genes on a chromosome. The mapping of the sequences to chromosomes is an important first step in correlating these sequences with genes associated with disease. The present inventors have mapped the genes encoding TREM-1 and TREM-2 to chromosome 6 in humans where NKp44 gene is also located.
Once a sequence has been mapped to a precise chromosomal location, the physical position of the sequence on the chromosome can be correlated with genetic map data. (Such data are found, for example, in V. McKusick, Mendelian Inheritance in Man, available on-line through Johns Hopkins University Welch Medical Library). The relationship between genes and disease, mapped to the same chromosomal region, can then be identified through linkage analysis (co-inheritance of physically adjacent genes), described in, e.g., Egeland etal., 1987, Nature, 325:783-787.
Moreover, differences in the DNA sequences between individuals affected and unaffected with a disease associated with a gene of the invention can be determined. If a mutation is observed in some or all of the affected individuals but not in any unaffected individuals, then the mutation is likely to be the causative agent of the particular disease.
Comparison of affected and unaffected individuals generally involves first looking for structural alterations in the chromosomes such as deletions or translocations that are visible from chromosome spreads or detectable using PCR based on that DNA sequence.
Ultimately, complete sequencing of genes from several individuals can be performed to confirm the presence of a mutation and to distinguish mutations from polymorphisms.
Furthermore, the nucleic acid sequences disclosed herein can be used to perform searches against "mapping databases", e.g., BLAST-type search, such that the chromosome position of the gene is identified by sequence homology or identity with known sequence fragments which have been mapped to chromosomes.
A polypeptide and fragments and sequences thereof and antibodies specific thereto can be used to map the location of the gene encoding the polypeptide on a chromosome. This mapping can be carried out by specifically detecting the presence of the polypeptide in members of a panel of somatic cell hybrids between cells of a=first species of animal from which the protein originates and cells from a second species of animal and then determining which somatic cell hybrid(s) expresses the polypeptide and noting the chromosome(s) from the first species of animal that it contains. For examples of this technique, see Pajunen et al. (1988) Cytogenet. Cell Genet. 47:37-41 and Van Keuren et al.
(1986) Hum. Genet. 74:34-40. Alternatively, the presence of the polypeptide in the somatic cell hybrids can be determined by assaying an activity or property of the polypeptide, for example, enzymatic activity, as described in Bordelon-Riser et al. (1979) Somatic Cell Genetics 5:597-613 and Owerbach etal. (1978) Proc. Natl. Acad. Sci. USA
75:5640-5644.
B. Tissue typing The nucleic acid sequences of the present invention can also be used to identify individuals from minute biological samples. The United States military, for example, is considering the use of restriction fragment length polymorphism (RFLP) for identification of its personnel. In this technique, an individual's genomic DNA is digested with one or more restriction enzymes, and probed on a Southern blot to yield unique bands for identification. This method does not suffer from the current limitations of "Dog Tags"
which can be lost, switched, or stolen, making positive identification difficult. The sequences of the present invention are useful as additional DNA markers for RFLP
(described in U.S. Patent 5,272,057).
Furthermore, the sequences of the present invention can be used to provide an alternative technique which determines the actual base-by-base DNA sequence of selected portions of an individual's genome. Thus, the nucleic acid sequences described herein can be used to prepare two PCR primers from the 5' and 3' ends of the sequences.
These primers can then be used to amplify an individual's DNA and subsequently sequence it.
Panels of corresponding DNA sequences from individuals, prepared in this manner, can provide unique individual identifications, as each individual will have a unique set of such DNA sequences due to allelic differences. The sequences of the present invention can be used to obtain such identification sequences from individuals and from tissue. The nucleic acid sequences of the invention uniquely represent portions of the human genome. Allelic variation occurs to some degree in the coding regions of these sequences, and to a greater degree in the noncoding regions. It is estimated that allelic variation between individual humans occurs with a frequency at about once per each 500 bases. Each of the sequences described herein can, to some degree, be used as a standard against which DNA from an individual can be compared for identification purposes.

Because greater numbers of polymorphisms occur in the noncoding regions, fewer sequences are necessary to differentiate individuals.
If a panel of reagents from the nucleic acid sequences described herein is used to generate a unique identification database for an individual, those same reagents can later be used to identify tissue from that individual. Using the unique identification database, positive identification of the individual, living or dead, can be made from extremely small tissue samples.
5.7.3 Diagnostic assays One aspect of the present invention relates to diagnostic assays for determining expression of a polypeptide or nucleic acid of the invention and/or activity of a polypeptide of the invention, in the context of a biological sample (e.g., blood, plasma, serum, cells, tissues) to thereby determine whether an individual is afflicted with a disease or disorder, or is at risk of developing a disorder, associated with aberrant expression or activity of a polypeptide of the invention, such as a proliferative disorder, e.g., psoriasis or cancer, or an angiogenic disorder. The invention also provides for prognostic (or predictive) assays for determining whether an individual is at risk of developing a disorder associated with aberrant expression or activity of a polypeptide of the invention. For example, mutations in a gene of the invention can be assayed in a biological sample. Such assays can be used for prognostic or predictive purpose to thereby prophylactically treat an individual prior to the onset of a disorder characterized by or associated with aberrant expression or activity of a polypeptide of the invention.
An exemplary method for detecting the presence or absence of a polypeptide or nucleic acid of the invention in a biological sample involves obtaining a biological sample from a test subject and contacting the biological sample with a compound or an agent capable of detecting a polypeptide or nucleic acid (e.g., mRNA, genomic DNA) of the invention such that the presence of a polypeptide or nucleic acid of the invention is detected in the biological sample. A preferred agent for detecting mRNA or genomic DNA
encoding a polypeptide of the invention is a labeled nucleic acid probe capable of hybridizing to mRNA or genomic DNA encoding a polypeptide of the invention. The nucleic acid probe can be, for example, a full-length cDNA, such as the nucleic acid of SEQ ID
NO:1 or 2, or a portion thereof, such as an oligonucleotide of at least 15, 20, 25, 30, 50, 100, 250, 500, or more contiguous nucleotides in length and sufficient to specifically hybridize under stringent conditions to a mRNA or genomic DNA encoding a polypeptide of the invention.
Other suitable probes for use in the diagnostic assays of the invention are described herein.

A preferred agent for detecting a polypeptide of the inventiorhis an antibody capable of binding to a polypeptide of the invention, preferably an antibody with a detectable label. Antibodies can be polyclonal, or more preferably, monoclonal. An intact antibody, or a fragment thereof (e.g., Fab or F(abg)2) can be used. See also the detailed descriptions about antibodies in section 5.5.
The term "labeled", with regard to the probe or antibody, is intended to encompass direct labeling of the probe or antibody by coupling (i.e., physically linking) a detectable substance to the probe or antibody, as well as indirect labeling of the probe or antibody by reactivity with another reagent that is directly labeled. Examples of indirect labeling include detection of a primary antibody using a fluorescently labeled secondary antibody and end-labeling of a DNA probe with biotin such that it can be detected with fluorescently labeled streptavidin. The term "biological sample" is intended to include tissues, cells and biological fluids isolated from a subject, as well as tissues, cells and fluids present within a subject. That is, the detection method of the invention can be used to detect mRNA, protein, or genomic DNA in a biological sample in vitro as well as in vivo.
For example, in vitro techniques for detection of mRNA include Northern hybridizations and in situ hybridizations. In vitro techniques for detection of a polypeptide of the invention include enzyme linked immunosorbent assays (ELISAs), Western blots, immunoprecipitations and immunofluorescence. In vitro techniques for detection of genomic DNA include Southern hybridizations. Furthermore, in vivo techniques for detection of a polypeptide of the invention include introducing into a subject a labeled antibody directed against the polypeptide. For example, the antibody can be labeled with a radioactive marker whose presence and location in a subject can be detected by standard imaging techniques.
In one embodiment, the biological sample contains protein molecules from the test subject. Alternatively, the biological sample can contain mRNA
molecules from the test subject or genomic DNA molecules from the test subject. A preferred biological sample is a peripheral blood leukocyte sample isolated by conventional means from a subject.
In another embodiment, the methods further involve obtaining a control biological sample from a control subject, contacting the control sample with a compound or agent capable of detecting a polypeptide of the invention or mRNA or genomic DNA
encoding a polypeptide of the invention, such that the presence of the polypeptide or mRNA
or genomic DNA encoding the polypeptide is detected in the biological sample, and comparing the presence of the polypeptide or mRNA or genomic DNA encoding the - 6? -. =

polypeptide in the control sample with the presence of the polypeptide or mRNA
or genomic DNA encoding the polypeptide in the test sample.
The invention also encompasses kits for detecting the presence of a polypeptide or nucleic acid of the invention in a biological sample (a test sample). Such kits can be used to determine if a subject is suffering from or is at increased risk of developing a disorder associated with aberrant expression of a polypeptide of the invention as discussed, for example, in sections above relating to uses of the sequences of the invention.
For example, kits can be used to determine if a subject is suffering from or is at increased risk of disorders such as immunological disorders, especially involving inflammatory disorders (e.g., bacterial infection, fungal infection, viral infection, protozoa or other parasitic infection, psoriasis, septicemia, cerebral malaria, inflammatory bowel disease, arthritis, such as rheumatoid arthritis, folliculitis, impetigo, granulomas, lipoid pneumoias, vasculitis, and osteoarthritis), autoimmune disorders (e.g., rheumatoid arthritis, thyroiditis, such as Hashimoto's thyroiditis and Graves' disease, insulin-resistant diabetes, pernicious anemia, Addison's disease, pemphigus, vitiligo, ulcerative colitis, systemic lupus erythematosus (SLE), SjOgren's syndrome, multiple sclerosis, dermatomiositis, mixed connective tissue disease, scleroderma, polymyositis, graft rejection, such as allograft rejection), T cell disorders (e.g., AIDS), allergic inflammatory disorders (e.g., skin and/or mucosal allergies, such as allergic rhinitis, asthma, psoriasis), neurological disorders, eye disorders, embryonic disorders, or any other disorders (e.g., tumors, cancers, leukemia, myeloid diseases, and traumas) which are directly or indirectly associated with aberrant TREM-1 and/or TREM-2 activity and/or expression.
The kit, for example, can comprise a labeled compound or agent capable of detecting the polypeptide or mRNA encoding the polypeptide in a biological sample and means for determining the amount of the polypeptide or mRNA in the sample (e.g., an antibody which binds the polypeptide or an oligonucleotide probe which binds to DNA or mRNA encoding the polypeptide). Kits can also include instructions for observing that the tested subject is suffering from or is at risk of developing a disorder associated with aberrant expression of the polypeptide if the amount of the polypeptide or mRNA encoding the polypeptide is above or below a normal level.
For antibody-based kits, the kit can comprise, for example: (1) a first antibody (e.g., attached to a solid support) which binds to a polypeptide of the invention;
and, optionally, (2) a second, different antibody which binds to either the polypeptide or the first antibody and is conjugated to a detectable agent.

For oligonucleotide-based kits, the kit can comprise, for exaMple: (1) an oligonucleotide, e.g., a detectably labeled oligonucleotide, which hybridizes to a nucleic acid sequence encoding a polypeptide of the invention or (2) a pair of primers useful for amplifying a nucleic acid molecule encoding a polypeptide of the invention.
The kit can also comprise, e.g., a buffering agent, a preservative, or a protein stabilizing agent. The kit can also comprise components necessary for detecting the detectable agent (e.g., an enzyme or a substrate). The kit can also contain a control sample or a series of control samples which can be assayed and compared to the test sample contained. Each component of the kit is usually enclosed within an individual container and all of the various containers are within a single package along with instructions for observing whether the tested subject is suffering from or is at risk of developing a disorder associated with aberrant expression of the polypeptide.
A. Prognostic Assays The methods described herein can furthermore be utilized as prognostic assays to identify a subject having or at risk of developing a disorder associated with aberrant expression or activity of a polypeptide of the invention, e.g., an immunologic disorder or other disorders as discussed above.
Furthermore, the prognostic assays described herein can be used to determine whether a subject can be administered an agent (e.g., an agonist, antagonist, peptidomimetic, protein, peptide, nucleic acid, small molecule, or other drug candidate) to treat a disease or disorder associated with aberrant expression or activity of a polypeptide of the invention. For example, such methods can be used to determine whether a subject can be effectively treated with a specific agent or class of agents (e.g., agents of a type which decrease activity of the polypeptide). Thus, the present invention provides methods for determining whether a subject can be effectively treated with an agent for a disorder associated with aberrant expression or activity of a polypeptide of the invention in which a test sample is obtained and the polypeptide or nucleic acid encoding the polypeptide is detected (e.g., wherein the presence of the polypeptide or nucleic acid is diagnostic for a subject that can be administered the agent to treat a disorder associated with aberrant expression or activity of the polypeptide).
The methods of the invention can also be used to detect genetic lesions or mutations in a gene of the invention, thereby determining if a subject with the lesioned gene is at risk for a disorder characterized aberrant expression or activity of a polypeptide of the invention. In preferred embodiments, the methods include detecting, in a sample of cells from the subject, the presence or absence of a genetic lesion or mutation characterized by at least one of an alteration affecting the integrity of a gene encoding the polypep-tide of the invention, or the misexpression of the gene encoding the polypeptide of the invention. For example, such genetic lesions or mutations can be detected by ascertaining the existence of at least one of the following: 1) a deletion of one or more nucleotides from the gene; 2) an addition of one or more nucleotides to the gene; 3) a substitution of one or more nucleotides of the gene; 4) a chromosomal rearrangement of the gene; 5) an alteration in the level of a messenger RNA transcript of the gene; 6) an aberrant modification of the gene, such as of the methylation pattern of the genomic DNA; 7) the presence of a non-wild type splicing pattern of a messenger RNA transcript of the gene; 8) a non-wild type level of a the protein encoded by the gene; 9) an allelic loss of the gene; and 10) an inappropriate post-translational modification of the protein encoded by the gene. As described herein, there are a large number of assay techniques known in the art which can be used for detecting lesions in a gene.
In certain embodiments, detection of the lesion involves the use of a probe/primer in a polymerase chain reaction (PCR) (see, e.g., U.S. Patent Nos.
4,683,195 and 4,683,202), such as anchor PCR or RACE PCR, or, alternatively, in a ligation chain reaction (LCR) (see, e.g., Landegran et al., 1988, Science, 241:1077-1080; and Nakazawa et at., 1994, Proc. Natl. Acad. Sci. USA, 91:360-364), the latter of which can be particularly useful for detecting point mutations in a gene (see, e.g., Abravaya et al., 1995, Nucleic Acids Res., 23:675-682). This method can include the steps of collecting a sample of cells from a patient, isolating nucleic acid (e.g., genomic, mRNA or both) from the cells of the sample, contacting the nucleic acid sample with one or more primers which specifically hybridize to the selected gene under conditions such that hybridization and amplification of the gene (if present) occurs, and detecting the presence or absence of an amplification product, or detecting the size of the amplification product and comparing the length to a control sample. It is anticipated that PCR and/or LCR may be desirable to use as a preliminary amplification step in conjunction with any of the techniques used for detecting mutations described herein.
Alternative amplification methods include: self sustained sequence replication (Guatelli etal., 1990, Proc. Natl. Acad. Sci. USA, 87:1874-1878), transcriptional amplification system (Kwoh, et al., 1989, Proc. Natl. Acad. Sci. USA, 86: 1 I
73- 1 1 7 7), Q-Beta Replicase (Lizardi et al., 1988, Bio/Technologv, 6: 1197), or any other nucleic acid amplification method, followed by the detection of the amplified molecules using techniques well known to those of skill in the art_ These detection schemes are especially useful for the detection of nucleic acid molecules if such molecules are present in very low numbers.

In an alternative embodiment, mutations in a selected gene frorn a sample cell can be identified by alterations in restriction enzyme cleavage patterns_ For example, sample and control DNA is isolated, amplified (optionally), digested with one or more restriction endonucleases, and fragment length sizes are determined by gel electrophoresis and compared. Differences in fragment length sizes between sample and control DNA
indicates mutations in the sample DNA. Moreover, the use of sequence specific ribozymes (see, e.g., U.S. Patent No. 5,498,531) can be used to score for the presence of specific mutations by development or loss of a ribozyme cleavage site.
In other embodiments, genetic mutations can be identified by hybridizing a sample and control nucleic acids, e.g., DNA or RNA, to high density arrays containing hundreds or thousands of oligonucleotides probes (Cronin et aL, 1996, Human Mutation, 7:244-255; Kozal et aL, 1996, Nature Medicine, 2:753-759). For example, genetic mutations can be identified in two-dimensional arrays containing light-generated DNA
probes as described in Cronin et al., supra. Briefly, a first hybridization array of probes can be used to scan through long stretches of DNA in a sample and control to identify base changes between the sequences by making linear arrays of sequential overlapping probes.
This step allows the identification of point mutations. This step is followed by a second hybridization array that allows the characterization of specific mutations by using smaller, specialized probe arrays complementary to all variants or mutations detected.
Each 70 mutation array is composed of parallel probe sets, one complementary to the wild-type gene and the other complementary to the mutant gene.
In yet another embodiment, any of a variety of sequencing reactions known in the art can be used to directly sequence the selected gene and detect mutations by comparing the sequence of the sample nucleic acids with the corresponding wild-type (control) sequence. Examples of sequencing reactions include those based on techniques developed by Maxim and Gilbert (1977, Proc. Natl. Acad. Sci. USA, 74:560) or Sanger (1977, Proc. Natl. Acad. Sci. USA, 74:5463). It is also contemplated that any of a variety of automated sequencing procedures can be utilized when performing the diagnostic assays (1995, Bio/Techniques, 19:448), including sequencing by mass spectrometry ( see, e.g., PCT Publication No. WO 94/16101; Cohen etal., 1996, Adv. Chromatogr., 36:127-162;
and Griffin et al., 1993, App!. Biochem. Biotechnol., 38:147-159).
Other methods for detecting mutations in a selected gene include methods in which protection from cleavage agents is used to detect mismatched bases in RNA/RNA or RNA/DNA heteroduplexes (Myers et al., 1985, Science, 230:1242). In general, the technique of "mismatch cleavage" entails providing heteroduplexes formed by hybridizing (labeled) RNA or DNA containing the wild-type sequence with potentially mutant RNA or DNA obtained from a tissue sample. The double-stranded duplexes are treated with an agent which cleaves single-stranded regions of the duplex such as which will exist due to basepair mismatches between the control and sample strands. RNA/DNA duplexes can be treated with RNase to digest mismatched regions, and DNA/DNA hybrids can be treated with SI nuclease to digest mismatched regions.
In other embodiments, either DNA/DNA or RNA/DNA duplexes can be treated with hydroxylamine or osmium tetroxide and with piperidine in order to digest mismatched regions_ After digestion of the mismatched regions, the resulting material is then separated by size on denaturing polyacrylamide gels to determine the site of mutation.
See, e.g., Cotton et al., 1988, Proc. Natl. Acad. Sci. USA, 85:4397; Saleeba et at., 1992, Methods Enzymol., 217:286-295. In a preferred embodiment, the control DNA or RNA can be labeled for detection.
In still another embodiment, the mismatch cleavage reaction employs one or more proteins that recognize mismatched base pairs in double-stranded DNA (so called "DNA mismatch repair" enzymes) in defined systems for detecting and mapping point mutations in cDNAs obtained from samples of cells. For example, the mutY
enzyme of E.
coli cleaves A at G/A mismatches and the thymidine DNA glycosylase from HeLa cells cleaves T at G/T mismatches (Hsu etal., 1994, Carcinogen esis, 15:1657-1662).
According to an exemplary embodiment, a probe based on a selected sequence, e.g., a wild-type sequence, is hybridized to a cDNA or other DNA product from a test cell(s).
The duplex is treated with a DNA mismatch repair enzyme, and the cleavage products, if any, can be detected from electrophoresis protocols or the like. See, e.g., U.S. Patent No. 5,459,039_ In other embodiments, alterations in electrophoretic mobility will be used to identify mutations in genes. For example, single strand conformation polymorphism (SSCP) may be used to detect differences in electrophoretic mobility between mutant and wild type nucleic acids (Orita et aL, 1989, Proc. Natl. Acad. Sci. USA, 86:2766; see also Cotton, 1993, Mutat. Res., 285:125-144; Hayashi, 1992, Genet. Anal. Tech.
App!., 9:73-79).
Single-stranded DNA fragments of sample and control nucleic acids will be denatured and allowed to renature. The secondary structure of single-stranded nucleic acids varies according to sequence, and the resulting alteration in electrophoretic mobility enables the detection of even a single base change. The DNA fragments may be labeled or detected with labeled probes. The sensitivity of the assay may be enhanced by using RNA
(rather than DNA), in which the secondary structure is more sensitive to a change in sequence. In a preferred embodiment, the subject method utilizes heteroduplex analysis to separate double stranded heteroduplex molecules on the basis of changes in electrophoretic mobility (Keen et al., 1991, Trends Genet., 7:5).

In yet another embodiment, the movement of mutant or wild-type fragments in polyacrylamide gels containing a gradient of denaturant is assayed using denaturing gradient gel electrophoresis (DGGE) (Myers et al. (1985) Nature 313:495). When DGGE is used as the method of analysis, DNA will be modified to insure that it does not completely denature, for example by adding a 'GC clamp of approximately 40 bp of high-melting GC-rich DNA by PCR. In a further embodiment, a temperature gradient is used in place of -a denaturing gradient to identify differences in the mobility of control and sample DNA
(Rosenbaum and Reissner, 1987, Biophvs. Cheni., 265:12753).
Examples of other techniques for detecting point mutations include, but are not limited to, selective oligonucleotide hybridization, selective amplification, or selective primer extension. For example, oligonucleotide primers may be prepared in which the known mutation is placed centrally and then hybridized to target DNA under conditions which permit hybridization only if a perfect match is found (Saiki et al., 1986, Nature, 324:163); Saiki et al., 1989, Proc. Natl. Acad. Sci. USA, 86:6230). Such allele specific oligonucleotides are hybridized to PCR amplified target DNA or a number of different mutations when the oligonucleotides are attached to the hybridizing membrane and hybridized with labeled target DNA.
Alternatively, allele specific amplification technology which depends on selective PCR amplification may be used in conjunction with the instant invention.
Oligonucleotides used as primers for specific amplification may carry the mutation of interest in the center of the molecule (so that amplification depends on differential hybridization) (Gibbs etal., 1989, Nucleic Acids Res. 17:2437-2448) or at the extreme 3' end of one primer where, under appropriate conditions, mismatch can prevent or reduce polym erase extension (Prossner, 1993, Tibtech,11:238). In addition, it may be desirable to introduce a novel restriction site in the region of the mutation to create cleavage-based detection (Gasparini etal., 1992 Mol. Cell Probes, 6:1). It is anticipated that in certain embodiments amplification may also be performed using Taq ligase for amplification (Barany, 1991, Proc. Natl. Acad. Sci. USA, 88:189). In such cases, ligation will occur only if there is a perfect match at the 3' end of the 5' sequence making it possible to detect the presence of a known mutation at a specific site by looking for the presence or absence of amplification.
The methods described herein may be performed, for example, by utilizing pre-packaged diagnostic kits comprising at least one probe nucleic acid or antibody reagent described herein, which may be conveniently used, e.g., in clinical settings to diagnose patients exhibiting symptoms or family history of a disease or illness involving a gene encoding a polypeptide of the invention. Furthermore, any cell type or tissue, e.g., preferably peripheral blood leukocytes, in which the polypeptide of the invention is expressed may be utilized in the prognostic assays described herein.
5.8 Methods of treatment 5.8.1 lmmunoregulatory effect of TREMs Inflammatory disorders are generally classified into two types; that is, acute and chronic inflammations. Acute inflammation is triggered by an initiating agent which is often a foreign substance, such as pathogenic organisms (e.g., bacteria, fungi, virus, protozoa and other parasites). The degradation products or toxins released by pathogens may directly cause activation of plasma proteases which leads to a series of inflammatory responses, including vasodilation, increased vascular permeability, recruitment and activation of neutrophils, monocytes, and eosinophils, and production of fever.
Furthermore, injured cells can release degradation products which trigger various plasma protease cascades, including complement, kinins, clotting and fibrinolytic proteins, lipid mediators, prostaglandins, leukotrienes, and platelet-activating factor. In addition, expression of proinflammatory cytokines, such as interleukin-1 (IL-1), IL-4, IL-6, IL-8, tumor necrosis factor (TNF) a and [3, interferon-y (IFN-y), and IL-12, is upregulated and the inflammatory responses are further augmented. The acute phase inflammatory responses are downregulated once the foreign threat is eliminated. Such downregulation is achieved by cell senescence or apoptosis (programmed cell death) which seems to be promoted by certain cytokines, including TNF-a, eicosanoids, IL-10, and antioxidants (Cox et al., 1996, Am J Physiol, 27:L566-L571; Gelrud etal., 1996, Proc Assoc Am Physicians, 108:455-456;
Gon etal., 1996, Microbiol Immunol, 40:463-465; Hebert etal., 1996, J Imunol, 157:3105-3115; Oishi et al.,1997, Scand J Immunol, 45:21-27), and by anti-inflammatory mediators, including 1L-4, transforming growth factor-n (TGF-13), IL-10, and IL-13, the latter three being released by macrophages and lymphocytes rather than by granulocytes.
However, if the elimination of the foreign substance is incomplete, the inflammatory process persists and chronic inflammation ensues. (See e.g., Rosenberg et at., 1999, Inflammation, in Fundamental Immunology, 4th Ed. W. E. Paul, ed. Lippincott-Raven, Philadelphia p. 1051).
As described in Examples below, the TREMs trigger cell activation, Ca2+
mobilization and tyrosine phosphorylation via an associated signal transduction molecule, called DAP12 (Lanier, L. L., 1998, NK cell receptors. Annu. Rev. Immunol., 16:359) (see Fig. 8 and section 6.8 below). Among TREMs, TREM-1 is exclusively expressed on neutrophils and monocytes and upregulated by bacterial and fungal stimuli (see sections 6.5.3 and 6.5.4, and Figures 6 and 14). TREM-1 triggers release of proinflammatory cytokincs and chemokines, such as tumor necrosis factor-a. (TNF-a), interleukin-8 (IL-8) and monocyte chemoattractant protein-1 (MCP-1), and induces degranulation of neutrophils in vitro as described in the section 6.7 below and Fig. 7. In addition, it renders neutrophils and monocytes highly resistant to spontaneous cell death in culture (unpublished). These observations strongly indicate that TREM-1 is involved in acute inflammatory reactions and, thus, prompted the present inventors to investigate its role in inflammation in vivo and its potential as a target for treatment of pathogenic hyperinflammatory conditions.
As presented in section 6.5.4 and Fig. 17, TREM-1 is strongly expressed on neutrophils associated with suppurative lesions of the skin caused by Staphylococcus aureus, such as folliculitis and impetigo and with suppurative granulomatous lymphadenitis I 0 caused by Bartonella henselae and Aspergillus fumigatus. Consistent with the role of TREM-1 in responses to bacterial infections, TREM-1 surface expression was strongly increased on infiltrating neutrophils isolated from the peritoneal cavity of patients with septic shock due to bacterial peritonitis (Fig. 19(b)) as well as that of the experimental mice having LPS-induced septic shock (Fig. 19(d)). On the other hand, TREM-1 is poorly expressed in neutrophilic infiltrates found in granulomatous lymphadenitis caused by sarcoid and foreign bodies granulomas and non-bacterial inflammatory reaction, including psoriasis, ulcerative colitis, and vasculitis caused by immune complexes (see Figs. 18 and Fig. 19(a)) and lipoid pneumonia. Furthermore, administration of soluble TREM-1 before (Figures 20 and 21(a)) or after (Fig. 21(c)) the injection of LPS protected mice from lethal endotoxemia.
On the other hand, TREM-2 is predominantly expressed on immature DCs (Figs. 25 and 26) and is essential for maturation of DCs as well as migration of DCs into lymph nodes to initiate adaptive immune responses (Fig. 36 and 37).
Furthermore, DAP12-deficient mice, which are also deficient in TREM-2 function, are more resistant to delayed hypersensitivity reaction (e.g., skin contact allergy) and experimental autoimmune encephalomyelitis (i.e., a mouse model for multiple sclerosis) due to reduced T cell stimulation by DCs (Bakker, A. B., Hoek, R. M., Cerwenka A., Blom, B., Lucian, L., McNeil, T., Murray, R., Phillips, L.H., Sedgwick, J. D., and Lanier L. L., 2000, DAP12-deficient mice fail to develop autoimmunity due to impaired antigen priming.
Immunity 13:345-53; Tomasello, E., Desmoulins, P. 0., Chemin, K., Guia, S., Cremer, H., Ortaldo, J., Love, P., Kaiserlian, D., and Vivier, E., 2000, Combined natural killer cell and dendritic cell functional deficiency in KASRAP/DAP12 loss-of-function mutant mice.
Immunity 13:355-64). Therefore, blocking TREM-2 with, for example, a soluble TREM-2 should reduce adaptive immune responses and protect the host from various immune disorders including autoimmunity and allergies.

Thus, TREM-1 ancUor TREM-2 are good targets for preventing-and treating various inflammatory disorders and diseases. Accordingly, the present invention provides for both prophylactic and therapeutic methods of treating a subject at risk of (or susceptible to) a disorder or having a disorder associated with aberrant expression or activity of a polypeptide of the invention, as discussed, for example, in sections above relating to uses of the sequences of the invention. For example, disorders characterized by aberrant expression or activity of the polypeptides of the invention include immunological disorders, especially involving inflammatory disorders (e.g., bacterial infection, fungal infection, viral infection, protozoa or other parasitic infection, psoriasis, septicemia, cerebral malaria, inflammatory bowel disease, arthritis, such as rheumatoid arthritis, folliculitis, impetigo, granulomas, lipoid pneumoias, vasculitis, and osteoarthritis), autoimmune disorders (e.g., rheumatoid arthritis, thyroiditis, such as Hashimoto's thyroiditis and Graves' disease, insulin-resistant diabetes, pernicious anemia, Addison's disease, pemphigus, vitiligo, ulcerative colitis, systemic lupus erythematosus (SLE), Sjogren's syndrome, multiple sclerosis, dermatomiositis, mixed connective tissue disease, scleroderma, polymyositis, graft rejection, such as allograft rejection), T cell disorders (e.g., AIDS) and allergic inflammatory disorders (e.g., skin and/or mucosa' allergies, such as allergic rhinitis, asthma, psoriasis), neurological disorders, eye disorders and embryonic disorders, or any other disorders (e.g., tumors, cancers, leukemia, myeloid diseases, and traumas) which are directly or indirectly associated with aberrant TREM-1 and/or TREM-2 activity and/or expression. The nucleic acids, polypeptides, and modulators thereof of the invention can be used to treat these disorders and diseases. Further, the nucleic acids, polypeptides, and modulators thereof of the invention can be co-administered with other therapeutics/prophylactics relevant to the diseases, e.g., anti-inflammatory agents, such as anti-TNF-a antibody, IL-1 receptor antagonist, anti-MIF antibody, and anti-HMG-antibody, and chemotherapeutic agents.
5.8.2 Prophylactic methods In one aspect, the invention provides a method for preventing in a subject, a disease or condition associated with an aberrant expression or activity of a polypeptide of the invention, by administering to the subject an agent which modulates expression or at least one activity of the polypeptide. Subjects at risk for a disease which is caused or contributed to by aberrant expression or activity of a polypeptide of the invention can be identified by, for example, any or a combination of diagnostic or prognostic assays as described herein. Administration of a prophylactic agent can occur prior to the manifestation of symptoms characteristic of thc aberrancy, such that a disease or disorder is _ .

prevented or, alternatively, delayed in its progression_ Depending on the type of aberrancy, for example, an agonist or antagonist agent can be used for treating the subject. The prophylactic agents described herein, for example, can be used to treat a subject at risk of developing disorders such as disorders discussed for example, in sections above relative to the uses of the sequences of the invention.
In a specific embodiment as described in section 6.10 (see also Figures 19(A) and (B)), mice were first treated with murine TREM-1-human IgG1 fusion protein (mTREM-1-IgG I ; 500 jig/animal) intraperitoneally. One (1) hour later, the mice were injected intraperitoneally with a lethal dosage of LPS (500 p.g/animal) to induce septic 1() shock. The intraperitoneal injection of LPS at this dosage leads to tissue damages, hemodynamic changes, multiple organ failure and death within 24 hours in control mice which have received control proteins (e.g., IgG1). Surprisingly, eighty (80) %
of the TREM-1 treated mice were protected from a lethal endotoxemia, only showing mild symptoms during the first few hours after LPS injection, and completely recovered within 4 days after LPS injection. It is presumed that the soluble form of TREM-1 acted as a decoy receptor for the ligands and prevented the latter from binding to the cell surface TREM-I.
Thus, the soluble form of TREM-1 demonstrated its effect as a prophylactic agent against septic shock.
5.8.3 Therapeutic methods Another aspect of the invention pertains to methods of modulating expression or activity of a polypeptide of the invention for therapeutic purposes. The modulatory method of the invention involves contacting a cell with an agent that modulates one or more of the activities of the polypeptide. An agent that modulates activity can be an agent as described herein, such as a nucleic acid or a protein, a naturally-occurring cognate ligand of the polypeptide, a peptide, a peptidomimetic, or other small molecule. In one embodiment, the agent stimulates one or more of the biological activities of the polypeptide.
Examples of such stimulatory agents include the active polypeptide of the invention and a nucleic acid molecule encoding the polypeptide of the invention that has been introduced into the cell. In another embodiment, the agent inhibits one or more of the biological activities of the polypeptide of the invention. Examples of such inhibitory agents include antisense nucleic acid molecules and antibodies. These modulatory methods can be performed in vitro (e.g., by culturing the cell with the agent) or, alternatively, in vivo (e.g., by administering the agent to a subject). As such, the present invention provides methods of treating an individual afflicted with a disease or disorder characterized by aberrant expression or activity of a polypeptide of the invention. In one embodiment, the method involves administering an agent (e.g., an agent identified by a screening assay -described herein), or combination of agents that modulates (e.g., upregulates or downregulates) expression or activity. In another embodiment, the method involves administering a polypeptide of the invention or a nucleic acid molecule of the invention as therapy to compensate for reduced or aberrant expression or activity of the polypeptide.
Stimulation of activity is desirable in situations in which activity or expression is abnormally low or downregulated and/or in which increased activity is likely to have a beneficial effect. Conversely, inhibition of activity is desirable in situations in which activity or expression is abnormally high or upregulated and/or in which decreased activity is likely to have a beneficial effect.
In a specific embodiment, the modulator is a soluble form of TREM-1 or TREM-2 molecule, for example, a fusion protein such as TREM-1-IgGI or TREM-2-IgM as described in the previous sections. The mTREM-1-IgGI or huTREM-1-IgGI (i.e., a fusion protein between mouse TREM-1 or human TREM-1 and human IgG1) successfully protected the mice from lethal endotoxemia when administered intraperitoneally up to two (2) hours after the LPS injection. Thus, TREM-1 can be used as a therapeutic agent when administered in an early phase of inflammation induced by LPS, presumably reducing the total amount of inflammatory mediators and preventing an irreversible tissue damages (also see section 6.10 and Fig. 21).
In another specific embodiment, an inhibitory antibody specific for TREM-I
or TREM-2 molecule can be used as a therapeutic agent_ Such antibodies would act as an antagonist against TREM-1 or TREM-2 by blocking the ligand-binding sites of and TREM-2 without triggering subsequent signal transduction reactions which lead to inflammatory disorders.
In another embodiment, nucleic acids comprising sequences encoding antibodies or fusion proteins, are administered to treat, prevent or ameliorate one or more symptoms associated with a disease, disorder, or infection, by way of gene therapy. Gene therapy refers to therapy performed by the administration to a subject of an expressed or expressible nucleic acid_ In this embodiment of the invention, the nucleic acids produce their encoded antibody or fusion protein that mediates a therapeutic or prophylactic effect.
Any of the methods for gene therapy available in the art can be used according to the present invention. Exemplary methods are described below.
For general reviews of the methods of gene therapy, see Goldspiel et al., 1993, Clinical Pharmacv,12:488-505; Wu and Wu, 1991, Biotherapy, 3:87-95;
Tolstoshev, 1993, Ann. Rev. Pharmacol. Toxicol., 32:573-596; Mulligan, 1993, Science, 260:926-932);
and Morgan and Anderson, 1993, Ann. Rev. Biochem., 62:191-217; May, 1993, TIB
TECH, 11(5):155-215. Methods commonly known in the art of recombinant DNA technology which can be used are described in Ausubel et al., (eds.), Current Protocols in Molecular Biology, John Wiley & Sons, NY (1993); and Kriegler, Gene Transfer and Expression, A
Laboratory Manual, Stockton Press, NY (1990).
In a preferred aspect, a composition of the invention comprises nucleic acids encoding a polypeptide or an antibody of the invention, or fragments thereof, said nucleic acids being part of an expression vector that expresses the polypeptide or antibody of the invention in a suitable host. In particular, such nucleic acids have promoters, preferably heterologous promoters, operably linked to the coding region of the polypeptide or antibody 1() of the invention, said promoter being inducible or constitutive, and, optionally, tissue-specific. In another particular embodiment, nucleic acid molecules of the invention are used in which the desired coding sequences are flanked by regions that promote homologous recombination at a desired site in the genome, thus providing for intrachromosomal expression of the polypeptide of the invention or fragments thereof (Koller and Smithies, 1989, Proc. Natl. Acad. Sci. USA, 86:8932-8935; and Zijlstra et al., 1989, Nature, 342:435-438).
In another preferred aspect, a composition of the invention comprises nucleic acids encoding a fusion protein, said nucleic acids being a part of an expression vector that expresses the fusion protein in a suitable host. In particular, such nucleic acids have promoters, preferably heterologous promoters, operably linked to the coding region of a fusion protein, said promoter being inducible or constitutive, and optionally, tissue-specific.
In another particular embodiment, nucleic acid molecules are used in which the coding sequence of the fusion protein and any other desired sequences are flanked by regions that promote homologous recombination at a desired site in the genome, thus providing for intrachromosomal expression of the fusion protein.
Delivery of the nucleic acids into a subject may be either direct, in which case the subject is directly exposed to the nucleic acid or nucleic acid-carrying vectors, or indirect, in which case, cells are first transformed with the nucleic acids in vitro, then transplanted into the subject. These two approaches are known, respectively, as in vivo or ex vivo gene therapy.
In a specific embodiment, the nucleic acid sequences are directly administered in vivo, where it is expressed to produce the encoded product.
This can be accomplished by any of numerous methods known in the art, e.g., by constructing them as part of an appropriate nucleic acid expression vector and administering it so that they become intracellular, e.g., by infection using defective or attenuated retroviral or other viral vectors (sec U.S. Patent No. 4,980,286), or by direct injection of naked DNA, or by use of microparticle bombardment (e.g., a gene gun; Biolistic, Dupont), or coating with lipids or cell-surface receptors or transfecting agents, encapsulation in liposomes, microparticles, or microcapsules, or by administering them in linkage to a peptide which is known to enter the nucleus, by administering it in linkage to a ligand subject to receptor-mediated endocytosis (see, e.g., Wu and Wu, 1987,1 Biol. Chem., 262:4429-4432) (which can be used to target cell types specifically expressing the receptors), etc. In another embodiment, nucleic acid-ligand complexes can be formed in which the ligand comprises a fusogenic viral peptide to disrupt endosomes, allowing the nucleic acid to avoid lysosomal degradation.
In yet another embodiment, the nucleic acid can be targeted in vivo for cell specific uptake and expression, by targeting a specific receptor (see, e.g., PCT Publications WO
92/06180; WO
92/22635; W092/20316; W093/14188; WO 93/20221). Alternatively, the nucleic acid can be introduced intracellularly and incorporated within host cell DNA for expression, by homologous recombination (Koller and Smithies, 1989, Proc. Natl. Acad. Sci.
USA, 86:8932-8935; and Zijlstra et al., 1989, Nature, 342:435-438).
In a specific embodiment, viral vectors that contain nucleic acid sequences encoding an antibody or a fusion protein are used. For example, a retroviral vector can be used (sec Miller et al., 1993, Meth. Enz_vmoL, 217:581-599). These retroviral vectors contain the components necessary for the correct packaging of the viral genome and integration into the host cell DNA. The nucleic acid sequences encoding the polypeptide of the invention, or fragments thereof, or a fusion protein to be used in gene therapy are cloned into one or more vectors, which facilitates delivery of the nucleotide sequence into a subject. Further details about retroviral vectors can be found in Boesen etal., 1994, Biotherapy, 6:291-302, which describes the use of a retroviral vector to deliver the mdr 1 gene to hematopoietic stem cells in order to make the stem cells more resistant to chemotherapy- Other references illustrating the use of retroviral vectors in gene therapy are: Clowes etal., 1994,1. Clin. Invest., 93:644-651; Klein etal., 1994, Blood, 83:1467-1473; Salmons and Gunzberg, 1993, Human Gene Therapy, 4:129-141; and Grossman and Wilson, 1993, Curr. Opin. in Genetics and Devel, 3:110-114.
Adenoviruses are other viral vectors that can be used in gene therapy.
Adenoviruses are especially attractive vehicles for delivering genes to respiratory epithelia_ Adenoviruses naturally infect respiratory epithelia where they cause a mild disease. Other targets for adenovirus-based delivery systems are liver, the central nervous system, endothelial cells, and muscle. Adenoviruses have the advantage of being capable of infecting non-dividing cells. Kozarsky and Wilson (Current Opinion in Genetics and Development, 3:499-503, 1993), present a review of adenovirus-based gene therapy. Bout et al., (Human Gene Therapy, 5:3-10, 1994) demonstrated the use of adenovirus vectors to transfer genes to the respiratory epithelia of rhesus monkeys. Other instances of the use of adenoviruses in gene therapy can be found in Rosenfeld etal., 1991, Science, 252:431-434;
Rosenfeld et at., 1992, Cell 68:143-155; Mastrangeli etal., 1993, J. C'/in.
Invest., 91:225-234; PCT Publication W094/12649; and Wang etal., 1995, Gene Therapy, 2:775-783. In a preferred embodiment, adenovirus vectors are used.
Adeno-associated virus (AAV) has also been proposed for use in gene therapy (see, e.g.,Walsh et al., 1993, Proc. Soc. Exp. Biol. Med., 204:289-300 and U.S.
Patent No. 5,436,146).
Another approach to gene therapy involves transferring a gene to cells in tissue culture by such methods as electroporation, lipofection, calcium phosphate mediated transfection, or viral infection. Usually, the method of transfer includes the transfer of a selectable marker to the cells. The cells are then placed under selection to isolate those cells that have taken up and are expressing the transferred gene. Those cells are then delivered to a subject.
In this embodiment, the nucleic acid is introduced into a cell prior to administration in vivo of the resulting recombinant cell. Such introduction can be carried out by any method known in the art, including but not limited to transfection, electroporation, microinjection, infection with a viral or bacteriophage vector containing the nucleic acid sequences, cell fusion, chromosome-mediated gene transfer, rnicrocellmediated gene transfer, spheroplast fusion, etc. Numerous techniques are known in the art for the introduction of foreign genes into cells (see, e.g., Loeffler and Behr, 1993, Meth. Enzymol., 217:599-618; Cohen etal., 1993, Meth. Enzymol., 217:618-644; and Clin. Pharma.
Ther., 29:69-92, 1985) and may be used in accordance with the present invention, provided that the necessary developmental and physiological functions of the recipient cells are not disrupted. The technique should provide for the stable transfer of the nucleic acid to the cell, so that the nucleic acid is expressible by the cell and preferably heritable and expressible by its cell progeny.
The resulting recombinant cells can be delivered to a subject by various methods known in the art. Recombinant blood cells (e.g., hematopoietic stem or progenitor cells) are preferably administered intravenously. The amount of cells envisioned for use depends on the desired effect, patient state, etc., and can be determined by one skilled in the art.
Cells into which a nucleic acid can be introduced for purposes of gene therapy encompass any desired, available cell type, and include but are not limited to epithelial cells, endothelial cells, keratinocytes, fibroblasts, muscle cells, hepatocytes; blood cells such as T lymphocytes, B lymphocytes, monocytes, macrophages, neutrophils, eosinophils, megakaryocytes, granulocytes; various stern or progenitor cells, in particular hematopoietic stem or progenitor cells, e.g., as obtained from bone marrow, umbilical cord blood, peripheral blood, fetal liver, etc.
In a preferred embodiment, the cell used for gene therapy is autologous to the subject.
In an embodiment in which recombinant cells are used in gene therapy, nucleic acid sequences encoding a polypeptide, an antibody or a fusion protein of the invention are introduced into the cells such that they are expressible by the cells or their progeny, and the recombinant cells are then administered in vivo for therapeutic effect. In a specific embodiment, stem or progenitor cells are used. Any stern and/or progenitor cells which can be isolated and maintained in vitro can potentially be used in accordance with this embodiment of the present invention (see e.g., PCT Publication WO
94/08598; Stemple and Anderson, 1992, Cell, 7 1:973-985; Rheinwald, 1980, Meth. Cell Bio., 21A:229; and Pittelkow and Scott, 1986, Mayo Clinic Proc., 61:771).
In a specific embodiment, the nucleic acid to be introduced for purposes of gene therapy comprises an inducible promoter operably linked to the coding region, such that expression of the nucleic acid is controllable by controlling the presence or absence of the appropriate inducer of transcription.
6. EXAMPLES
The following examples illustrate the cloning, production, isolation, and characterization of TREMs and fusion proteins thereof, and antibodies. These examples should not be construed as limiting.
A. METHODS
6.1 Cloning of TREM cDNAs GenBank expressed sequence tagged database (dbEST) was searched with the amino acid sequences of NKp44 using the tblastn algorithm, and several overlapping cDNAs were found. A contig assembled from 17 distinct cDNAs (accession nos.
D78812, A1337247, AW139572, AW274906, AW139573, A1394041, A1621023, A1186456, A1968134, A1394092, A1681036, A1962750, AA494171, AA099288, AW139363, AW135801, AA101983) containcd an open reading frame encoding a protein of 234 amino acids, referred to as TREM-1, with a predicted molecular mass of ¨26 kDa (Fig.
1(a)).
Search of the dbEST with the complete TREM-1 open reading frame matched to one related sequence referred to as TREM-2 (accession no. N41388) (Fig. 1(b)). TREM-1 (Fig. 2) and TREM-2 (Fig. 3) sequences have been submitted to GenBank database underaccession nos.
AF196329 and AF213457, respectively.
6.2 RT-PCR
The ¨760-bp TREM-1 and ¨1000-bp TREM-2 cDNAs were amplified by RT-PCR (reverse transcription PCR), cloned into pCR2.1 (Invitrogen, Carlsbad, CA), and sequenced. PCR primers were: TREM-I, 5'-GCTGGTGCACAGGAAGGATG (SEQ ID
NO:5), 3'-GGCTGGAAGTCAGAGGACATT (SEQ ID NO:6); and TREM-2, 5t-TGATCCTCTCTTTTCTGCAG (SEQ ID NO:7), 3'-GTGTTTAAAATGTCCAATATT
(SEQ ID NO:8).
6.3 Production of Fusion Proteins and Monoclonal Antibodies (mAb) 6.3.1 Production of huTREM-1-IgG1 and anti-TREM-1 mAb To produce soluble huTREM-1 -IgGl, the cDNA fragment encoding the huTREM-I extracellular region was amplified by PCR and cloned into an expression vector containing the exons for hinge, CH2, and CH3 region of human IgGI.
Transfection of the chimeric gene into the mouse myeloma cell line J558L, screening of culture supernatants, and purification of huTREM-1-IgG I were performed as previously described (Traunecker et al., 1991, Myeloma based expression system for production of large mammalian proteins, Trends Biotechnol., 9:109). Briefly, the huTREM-1-IgGI plasmid was transfected into J558L mouse myeloma cells by electroporation and cells were cultured in DMEM
supplemented with 2mM L-glutamine, 1% non-essential amino acids, 1% sodium pyruvate, 50mg/m1 kanamycin. After two days of culture, selective medium containing 4mg/m1 mycophenolic acid (Calbiochem) and 125mg/m1xanthine (Sigma) was added and incubation at 37 C continued until resistant colonies appeared. Clones were screened for production of soluble IgG fusion proteins by enzyme-linked immunosorbent assay (ELISA) using a goat anti-human IgG antibody. Producer clones were expanded, while the FCS
content was diminished to 2%. For purification of the fusion protein, culture supernatant was concentrated and adsorbed over a recombinant protein A column (Repligen, Cambridge, MA). After washing with PBS-0.02% sodium azide, the bound fusion protein was eluted with 0.1M glycine-HC1, pH 2.65. One (1)-ml fractions were collected in test tubes containing 100m1 2MTris-HC1, pH 8, pooled, and dialyzed against PBS.
Purified protein was then concentrated, sterile-filtered and kept frozen. Anti-huTREM-1 mAbs were produced by immunizing BALB/c mice with huTREM-1-IgG1 and preparing hybridomas using a standard hybridoma technique as reported elsewhere (Cella et al., 1997, A novel inhibitory receptor (I LT3) expressed on monocytes, macrophages, and dendritic cells involved in antigen processing. J. Exp. Med., 185:1743). One of the anti-TREM-antibodies was designated as 21C7 mAb (IgG1,x).
6.3.2 Production of huILT3-IgG1 and mTREM-1-IgG1 fusion proteins Human ILT3-IgG1 (huILT3-IgG1) was cloned, produced and purified as described for huTREM-1-IgG1 above. To produce murine TREM-I (mTREM-1) as a soluble fusion protein, a chimeric gene consisting of the mTREM-1 extracellular domain and human IgG1 constant regions was constructed. The cDNA fragment encoding the mTREM- I extracellular region was amplified by PCR from cloned plasmid DNA.
The forward primer contained an EcoRI restriction site and the TREM-1 start codon:
5'-TAGTA
GGAATTCAGGATGAGGAAGGCTGGG (SEQ ID NO:29). The reverse primer provided a HindIII restriction site, a splice donor sequence, and several mTREM-1 codons preceding the transmembrane domain: 3'-TAGTAGAAGCTTATACTTACCGTCAGCATCTGTCC
CATTTAT (SEQ ID NO:30). The ¨640-bp PCR product was cut with EcoRI and HindlII, and ligated into an expression vector containing the exons for hinge, CH2 and CH3 regions of human IgGl, the guanosine phosphotransferase gene conferring resistance to mycophenolic acid, and the k promoter for the expression in the mouse myeloma cell line 1558L. Transfection, screening of culture supernatants and purification of mTREM-1-IgG1 were performed as described above.
Anti-mTREM-1 and anti-huILT3 mAbs were prepared using these fusion proteins according to the method described above and one of the anti-mTREM-1 clones was designated as 50D1 (rat IgGl, k) and one of the anti-huILT3 clones as ZM3.8 (murine IgGl, k).
6.3.3 Quantification of Human IgG1 Fusion Proteins Purified human IgGI fusion proteins were assayed for specificity, titer and functionality by ELISA using Protein A as a capturing protein and, either goat anti-human IgGI-HRP-conjugated polyclonal antibody (pAb; SBA) or specific biotinylated mAb against huTREM-1(21C7, murine IgG1,x), mTREM-1(50D1, rat IgG1,x), or ILT3 (ZM3.8, murine IgG1,x) followed by streptavidin-HRP. Immunoblot analysis of purified human IgGI fusion proteins revealed only one band of immunoreactivity.
6.3.4 Production of huTREM-2-IgM and anti-TREM-2 mAb To produce TREM-2 as a soluble fusion protein, a chimeric gene encoding the human TREM-2 extracellular domain and human IgG I constant regions was first constructed. The cDNA fragment encoding the TREM-2 extracellular region was amplified by PCR from cloned plasmid DNA. The forward primer 5'-TAGTAGGAATTCACT-_ CTGCTTCTGCCCTTGGCTGGGG (SEQ ID NO:31) contained an EcoRI restriction site and 25 nucleotides preceding the TREM-2 start codon. The reverse primer 3'-TAGTAG-AAGCTTATACTTACCGGGTGGGAAAGGGATTTCTCCTTCCAA (SEQ ID NO:32) provided a Hindu l restriction site, a splice donor sequence, and several TREM-2 codons preceding the transrnembrane domain. The ¨600-bp PCR product was cut with EcoRI and HindIII, and ligated into an expression vector containing the exons for hinge, CH2 and CH3 regions of human IgG I . TREM-2 extracellular region together with the splice donor site was re-amplified using the same forward primer (SEQ ID NO:31) and a reverse primer containing a Sall site and the splice donor sequence 3'-ACCTGCAGGCATGCGTCGA-CATACTTACC (SEQ ID NO:33).- The ¨600-bp PCR product was cut with Sall and ligated into an expression vector containing the exons for hinge, CH2, CH3 and CH4 regions of human IgM, the guanosine phosphotransferase gene conferring resistance to mycophenolic acid, and the k promoter for the expression in the mouse myeloma cell line J558L.
Purification of huTREM-2-IgM from culture supernatants was performed using KAPTIV-M-Sepharose according to manufacturer's protocols (Tecnogen, Milano).
For the production of anti-TREM2 mAb, 6-week-old BALB/c mice (Iffa-Credo, Larbresle, France) received an initial subcutaneous injection of 100 jig purified huTREM-2-IgM in Freund's complete adjuvant (FCA) behind the neck. The second immunization subcutaneously behind the neck (100 jig purified huTREM-2-IgM in Freund's huTREM-2-IgM in PBS) were performed in one-week intervals. Three days after the final booster immunization, spleen cells were isolated and fused with the myeloma cells. Hybridoma supernatants were screened by ELISA using huTREM-2-IgM
as coating protein and human immunoglobulin-adsorbed goat-anti-mouse IgG
labeled with horseradish peroxidase (PharMingen, San Diego, CA) as detecting antibody.
Supernatants from positive clones were then tested by flow cytometry for their ability to bind to immature DCs and 293 cells that were transiently transfected with flag-tagged TREM-2.
One of the anti-TREM-2 antibodies was designated as 29E3 mAb (IgG1 oc) (see Fig. 24).
6.3.5 Preparation of Fab/F(abl2 Fragments Monoclonal antibodies, 29E3 (anti-TREM-2; IgG1,x), 21C7 (anti-TREM-1;
IgG1,x), and 1B7.11 (anti-2,4,6 TNP, American Type Culture Collection; control IgG1 ,x) were purified using GammaBind-Sepharose (Pharmacia). The purified mAb were either biotinylated (Molecular Probes, Eugene, OR) or labeled with Cy5 (Pharrnacia) according to manufacturer's protocols. In addition, Fab or F(ab')2 fragments of mAb 29E3 and mAb 21C7 were prepared using thc Fab'/F(ab')2 Kit from Pierce Chemical (Rockford, IL). Fab and F(ab')2 fragments were subsequently biotinylated thus allowing cross-linking by ExtrAvidine (Sigma) or FACS analysis using Streptavidine-APC or -PE
(Pharrningen).
Functional characterization of Fab and F(ab1)2 29E3Bi'in were analyzed by FACS
for cell surface expression of TREM-2 (see Fig. 30; grey profile and solid bold profile, respectively). TREM-1 is not detectable on monocyte-derived DCs with Fab or F(ala')2 9E2"'' followed by Streptavidine (Fig. 30; dashed profile).
6.4 Transient Transfections HuTREM-1 and huTREM-2 were subcloned into pCMV-1-FLAG (Kodak) and expressed as amino-terminal FLAG peptide fusion proteins in COS-7 cells or 293 cells.
DAP12 was subcloned into pHM6 (Boehringer Mannheim, Mannheim, Germany) and expressed as amino-terminal hemagglutinin (HA) peptide fusion protein in COS-7 cells.
Transient transfections were performed as previously described (Nakajima et aL, 1999, Human myeloid cells express an activating ILT receptor (ILT1) that associates with Fc receptor 7-chain, J. ImmunoL, 162:5). Cell surface expression of transfected cDNAs was determined by FACS analysis with anti-FLAG (Kodak), anti-HA (Boehringer Mannheim), 21C7 mAb, and 29E3 mAb.
As shown in Fig. 4, mAb 21C7 stained TREM-1-transfected COS-7 cells, as compared with control transfectants (lower right quadrant). In addition, expression of TREM-1 was partially increased by cotransfection of DAP12 cDNA (Fig. 4(b)), suggesting that cell surface expression of TREM-1 may require association with either DAP12 or a related signaling molecule.
As shown in Fig. 24, 29E3 mAb specifically recognized TREM-2 . The 293 cells expressing TREM-2I:LAG (b and d) and those expressing TREM-1FLAG (a and c) were stained with 29E3(C and d). 24.1% of TREM-2FLAG cells and 0.98% of TREM-1 FLAG
cells (upper right quadrant) were stained with 29E3 mAb. Expression of TREM-1 FLAG
and TREM-2'G was confirmed using anti-FLAG mAbs (a and b). Staining with an isotype-matched control mAbs was set to the indicated lower quadrant.
6.5 Cells 6.5.1 Isolation of human monocytes, neutrophils, and dendritic cells Human blood was mixed with one volume of 3% Dextran T-500 (Pharmacia, Uppsala, Sweden) in 0.9% NaCI and left for sedimentation (30 min) to remove erythrocytes.
Leukocytes in the supernatant were farther_separated by gradient density centrifugation on TM
Lymphocyte Separation Medium (1CN Biomedicals/Cappcl, Aurora, OH) into peripheral blood mononuclear cells (PBMCs) and neutrophils. The pelleted neutrophils were further purified form contaminating erythrocytes by hypotonic treatment with 0.2% NaCI
solution for 30 sec. CD14+ monocytes were purified from PBMCs by magnetic cell sorting using TM
CD14 MicroBeads (Miltenyi, Bergisch Gladbach, Germany).
Monocyte-derived dendritic cells (DCs) were prepared from purified monocytes cultured in GM-CSF and TNF-a for 10 days as described by Sallust , F. and Lanzavecchia, A. (1994, Efficient presentation of soluble antigen by cultured human dendritic cells is maintained by granulocytes/macrophage colony-stimulating factor plus interleukin 4 and downregulated by tumor necrosis factor alpha. I Exp. Med.
179:1109-18).
63.2 Surface biotinylation and pervanadate treatment Monocytes or Monocyte-derived DCs were washed three times in PBS
followed by incubation with Sulfo-NHS-Biotin according to the manufacturer's protocol (Pierce). For pervanadate treatment, cells were incubated with 200pM
pervanadate and 200 p.M H202 at 37 C for 5min. Biotinylation or Pervanadate stimulation was stopped by washing the cells 3 times or 1 time, respectively, with ice cold PBS.
6.5.3 Human peritoneal leukocytes Human peritoneal leukocytes were obtained from peritoneal lavage of 20. patients diagnosed with aseptic Systemic Inflammatory Response Syndrome or polymicrobial sepsis, as defined by the Consensus Conference of the American College of Chest Physicians and Society of Critical Care Medicine (American College of Chest Physicians/Society of Critical Care Medicine Consensus Conference: definitions for sepsis and organ failure and guidelines for the use of innovative therapies in sepsis_ Crit. Care.
Med. 20:864-74, 1992).
6.6 Staining and FACS Analysis 6.6.1 Human cell distribution study of TREM-1 Before staining, all cells were incubated with 20% human serum in PBS for 1 hour on ice to block Fc receptors. Whole blood leukocytes were incubated with mAbs 21 C7 (anti-TREM- 1, IgG I), 3C10 (anti-CD14, IgG2b), and L243 (anti-HLA-DR, IgG2a) followed by isotype-specific FITC/PE/biotin-conjugated secondary Abs. After a further incubation step with APC-labeled streptavidin, cells were analyzed by FACS.
Monocytes and neutrophils stimulated with LPS (I rig/m1) for 16 hours were stained with either inAb 21C7 or mAb 1137.11 (control IgG1 , anti-2,4,6-trinitrophenyl (TNP); American Type Culture Collection, Manassas, VA), followed by human immunoglobulin-adsorbed PE-conjugated goat anti-mouse IgG (Southern Biotechnology Associates, Birmingham, AL). See Figures 5 and 6.
Monocytes stimulated with proinflammatory cytokines, TNF-a (20 ng/m1), IL-113 (20 ng/ml), TGE13 (20ng/mI), or IL-10 (20 nWrn1) for 24 hours were also stained as described above. See Fig. 15.
6.6.2 Effects of bacterial products on human cells Purified Monocytes and Neutrophils were cultured in the absence or presence of Lipopolysaccharide (100 ng/m1), Lipoteichoic acid (LTA; 100 ng/ml) or mycolic acid (10 jig/m1). Before staining, all cells were preincubated with 20% human serum in PBS
for 1 hour on ice to block Fc receptors. After incubation with either mAb 21C7 (1gGl, anti-TREM-1) or mAb 1B7.11 (control IgGl, anti-2,4,6 TNP, American Type Culture Collection), and a second-step human immunoglobulin-adsorbed phycoerythrin (PE)-conjugated goat anti-mouse IgG, cells were analyzed on a FACSCalibuTrmcytometer using M
CELLquestT software (Beckton Dickinson & Co., Palo Alto, CA). See Figures 6 and 14(B).
6.6.3 Regulation of TREM-1 durin2 bacterial infections To explore how bacterial infections affect the expression of TREM-1 on neutrophils and monocytes, these cells were exposed to various types of bacteria and the degrees of TEM-1 expression were compared to that of non-exposed control cells.
Staphylococcus aureus, Pseudornonas aeruginosa, and Bacillus of Calmette-Guerin (BCG) were cultured to the logarithmic growth phase based on the growth curves.
The bacterial cells were theft collected and washed twice in PBS.
Subsequently, the bacterial cells were incubated at 80 C for 30 min to be heat-inactivated.
Purified human neutrophils and monocytes were incubated with the heat-inactivated bacteria whose concentration was within the range for the optimal upregulation of TREM-1 (i.e., monocytes/neutrophils : bacteria = 1:10 - 1:100). The resulting cell surface expression of TREM-1 was assessed by flow cytometry with the 21C7 mAb as described in section 6.5.2. See Fig. 14(A).
Four-colour analysis of human peritoneal leukocytes was performed using anti-TREM-1, anti-CD15 (Immunotech, Marseille), anti-CD14 (Immunotech) and (Immunotech) monoclonal antibodies conjugated with Allophycocyanin (APC), CyChromTem, Phycoerythrin (PE) and Fluorescein isothiocyanate (F1TC), respectively. See Figs. I9(a) and (b).

6.6.4 Human cell distribution study of TREM-2 Before staining, all cells were preincubated with PBS-20% human serum for 1 hour on ice to block Fc receptors (FcR). Monocytes cultured in M-CSF, GM-CSF/IL4, IL-4, and GM-CSF were stained with either mAb 29E3 (Fig. 25), mAb 21C7, or mAb 1B7.11 (anti-2,4,6 TNP), followed by human immunoglobulin-adsorbed goat anti-mouse IgG conjugated with phycoerythrin (PE). In three-color staining, immature DCs cultured with LPS (10Ong/m1), TNF-ct (1Ong/m1), or CD4OL-transfected mouse myeloma cells (Lane, P., Burdet, C., McConnell, F., Lanzavecchia, A., and Padovan, E., 1995, CD40 ligand-independent B cell activation revealed by CD40 ligand-deficient T cell clones:
evidence for distinct activation requirements for antibody formation and B
cell proliferation.
Eur. J. Inununol. 6:1788) were incubated with Cy5-labeled 29E3 mAb, Fluoresceine-Isothiocyanate (FITC)-conjugated anti-CD83 mAb (Immunotech, Marseille, France), and PE-conjugated anti-MHC class II mAb (Immunotech). Cells were analyzed on a FACSCalibur cytometer using CELLquest software (Beckton Dickinson & Co., Palo Alto, CA). Dead cells were excluded by gating on propidium iodide (PI)-negative cells (i.e., live cells).
6.7 Immunohistochemical study Normal tissue samples included two lymph nodes showing non specific reactive change and three skin biopsies without obvious abnonnalities. In addition, two spleens showing extramedullary hematopoiesis were analyzed. Pathological samples included nine cases of infectious epithelioid cell granulomas, three cases of sarcoidosis (lymph node), three cases of lipoid pneumonia, four cases of psoriasis and two skin biopsies affected by Staphylococcus aureus infection, with features of impetigo and folliculitis. The infectious granulomas were localized in lymph nodes (8 cases) and mediastinum (1 case) and were caused by Mycobacterium tuberculosis (three cases), Bartonella henselaelcat scratch disease (five cases), and Aspergillusfionigatus; the latter belonged to a patient affected by Chronic Granulomatous Disease. Finally, a foreign-body giant-cell reaction associated with a vascular plastic prostheses, which was removed because of thrombosis, was analyzed. Except for sarcoidosis (all cases), tuberculosis (1 case) and the foreign-body granuloma, all other granulomas were characterized by variable degrees of suppurative inflammation, that was particularly prominent in the Bartonella henselae and Aspergillus , fianigatus infections. All tissues were freshly frozen in liquid nitrogen-precooled isopcntane and stored at -80 C. Immunostaining with 21C7 was performed on frozen sections, applying the antibody at the concentration of-1 jig/m1; an isotypc-matched antibody (1gG 1) was used as negative control. Anti-CD15 (Dako, Milan, Italy) antibodies were also applied to identify neutrophils. Immunostaining followed the streptavidin-biotin immunoperoxidase technique (Facehetti etal., 1992, Suppurative granulomatous lymphadenitis: Immunohistochemical evidence for a B-cell-associated granuloma.
Am. J.
Surg. Pathol. 16:955-61). Endogenous peroxidase was inhibited by pre-incubating the sections with 0.00 I% H202 methanol solution; chrornogenic reaction was developed with 3-amino-9-ethylcarbazole (AEC), and nuclei were counterstained with Mayer's hematoxylin. See Figures 1 7 and 18.
6.8 Measurement of Cytokines, Chemokines, Degranulation, and Cell Surface Activation Markers 6.8.1 Stimulation of TREM-1 To examine whether TREM-I can trigger acute inflammatory responses, purified monocytes or neutrophils were stimulated for 24 h in 96-well flat-bottom plates coated with F(abi)2 goat anti-mouse IgG (5 pig/m1) followed by either 21C7, 1F11 (anti-MHC class I), or 1B7.11 (anti-2,4,6 TNP) mAbs. Cells were plated at a concentration of 5 x 104 cells/well in the presence or absence of LPS (1 ig/m1). Supernatants were collected and tested for production of IL-6, IL-8, IL-10, IL-12p75, monocyte chemoattractant protein-1 (MCP-1), TNF-a, and myeloperoxidase (MPO) by ELISA (PharMingen, San Diego, CA).
See Figures 7(A)-(1-1). To measure the expression of cell surface markers, monocytes and neutrophils were stimulated as described above and, after 48 hours, were stained with PE-or FITC-conjugated anti-CD lib, anti-CD1 1 c, anti-CD18, anti-CD29, anti-CD32, anti-CD40, anti-CD49d, anti-CD49e, anti-CD54, anti-CD80, anti-CD83, or anti-CD86, (all from Immunotech, Marseille, France) and analyzed by FACS. See Table I below.
6.8.2 Stimulation of TREM-2 Immature DCs 5x105 cells/well were stimulated for 36 hours in 24-well flat-bottom plates coated with Fab 29E3 or Fab 21C7 (20 gimp. Supernatants were collected and tested for production of IL-6, 1L-8, IL-10, IL-12p75, 1L-13, IL-16, IL-18, IL-la, IL-113, TNF-a, and MCP-1 by ELISA (PharMingen). See Table II. Type I IFN was measured by evaluating the inhibition of Daudi cell proliferation with reference to a standard IFN-a curve (Nedennan, T., Karlstrom, E., and Sjodin, L., 1990, An in vitro bioassay for quantitation of human interferons by measurement of antiproliferative activity on a continuous human interferons by measurements of antiproliferative activity on a continuous human lymphoma cell line. Biologicals 18:29-34). The sensitivity of the assay was 0.2 U/ml. To measure stimulation-dependent changes in the expression of cell surface markers, monoeyte-derived DCs were stimulated with F(ab')2 control mAb (anti-TREM-1), F(a131) anti-TREM-2 mAb, or LPS. After different time periods (6, 12, 24, and 48 hours), cells were harvested, stained with mouse anti-CCR7 IgM mAb (Pharmingen, San Diego, CA) (see Fig. 36), followed by PE-labeled anti-mIgM Ab or PE- or FITC-conjugated anti-MHC
class I, -MHC class II, -CD1a, -CD1 I a, CD11b, CD I 1 c, -CD29, -CD31, -CD32, -CD35, -CD40, -CD41, -CD54, -CD6 I , -CD80, -CD83, -CD86, -CD89, -CDI 03, -CD115, -CD116, -CCR5, -CCR6, -CXCR4, (all from Immunotech) and analyzed by FACS (see Table III

below). In experiments where kinase inhibitors (PD98059 (50pg/m1): Erk inhibitor; or LY294002 (101g/m1): PI 3 kinase inhibitor, both from Calbiochem, San Diego, CA) or serine protease inhibitor (TPCK (15 jig/m1): NFkB-activation inhibitor; Sigma, St. Louis, MO) were used, the inhibitors were added 60 min before stimulation.
6.9 Measurement of Cytosolic Ca2+ and Tyrosine-Phosphorylated Proteins Determination of intracellular Ca2+ mobilization was done according to the previous reports (Nakajima et at., 1999, Human myeloid cells express an activating ILT
receptor (ILT1) that associates with Fc receptor y-chain,J. ImmunoL, 162:5).
Briefly, monocytes or monocyte-derived DCs were loaded with Indo-1 AM dye (Sigma) for 30 min at 37 C, washed 3 times and resuspended in RPMI-10mM HEPES/10%FCS. Cytoplasmic Ca2-` levels were monitored in individual cells by measuring 405/525 spectral emission ratio of loaded Indo-1 dye by flow cytometry (Nalcajima el aL, supra; Yamashita et at., 1998, Inhibitory and stimulatory functions of paired Ig-like receptor (PIR) family in RBL-2H3 cells, J. ImmunoL, 161:4042). After obtaining the baseline for at least 30 seconds, for TREM1 stimulation, anti-TREM-1 mAb or anti-MHC class I (isotype-matched control mAb) and a cross-linking Ab (goat anti-mouse IgG) were added to the monocytes, and analysis was allowed to continue (see Fig. 8). For TREM2 stimulation, either 29E3Bi06"
(1gGloc or Fab) or 21C7Bi 6" (IgGI,x or Fab) was added to a final concentration of 1 pg/ml and analysis was continued up to 512 sec (see Fig. 31). In some experiments, ExtraAvidinr (Sigma) was added as cross-linker together with the biotinylated primary antibodies or antibody fragments.
Determination of protein tyrosine phosphorylation, mitogen activated protein kinase activation, phospholipase C-y (PLC-y) phosphorylation, and immunoprecipitations was performed as previously described (Dietrich et aL, 2000, Signal-regulatory protein fu is a DAP12-associated activating receptor expressed in myeloid cells, J. ImmunoL, 164:9).
Briefly, monocytes were incubated at 37 C with 27C1 mAb (anti-TREM-1) or control IgG1 (anti-MHC class I) mAbs in the presence of a cross-linking Ab for the indicated time periods. After stimulation, an aliquot of the cells was lysed and subjected to anti-phosphotyrosine blotting using PY-20 (Transduction Laboratories, Lexington, KY) (see Fig. 9). Likewise, anti-phosphotyrosine blot of cell lysates from monocyte-derived DCs stimulated with F(ab')2 29E3 (anti-TREM-2) or control F(abi)2 9E2 (anti-TREM-1) are shown in Fig. 32.
Another aliquot of stimulated monocytes or monocyte-derived DCs was examined by Western blot analysis using anti-phospho-extracellular signal-regulated kinase (P-ERK1/2) (Figs. 10(a) and 33(a)) and anti-ERK1/2 mAbs (Figs. 10(b) and 33(b)).
Tyrosine phosphorylated proteins were precipitated from the stimulated monocyte lysates and immunoblotted with anti-PLC-y (Fig. 10(c)) or anti-Hck (Fig. 10(d)) Abs. An anti-Hck blotting was performed as a loading control because phosphorylation of Hck is similar in both stimulated and unstimulated monocytes. Phosphorylated proteins are indicated by arrows in all panels. Molecular weight markers are shown.
6.10 Immunoprecipitation Surface-biotinylated cells were lysed in 1% digitonin, 100mM Tris-HC1pH
7.4, 150mM NaC1, protease inhibitors (Complete, Roche, Switzerland'. After overnight TM
preclearing with normal mouse serum coupled to protein G Sepharose 4B, lysates were subjected to immunoprecipitation with 5pg/m1 of 29E3, 21C7, 1F11 (anti-MHC
class I
mAb), or 187.11 at 4 C for 3 hours. Immunecomplexes were precipitated by addition of Protein-G-Sepharose 48 FastFlow (Pharmacia) for 3 hours at 4 C. Precipitates were washed 4 times with lysis buffer, followed by a final wash with 0.5%
digitonin, 100mM
Tris-HC1 pH7.4, 150mM NaCl. Elution from sepharose occurred under reducing or non-reducing conditions using standard SDS-PAGE sample buffer. After separation by SDS-PAGE, precipitate set up was analyzed by Western Blot with Horseradish-Peroxidase (HRP)-conjugated Streptavidine. In deglycosylation experiments the precipitates were incubated for 18 hours with or without N-Glycanase F (Boehringer Mannheim) according to the manufacturer's protocol (see Fig. 11 and Fig. 27).
In another experiment, pervanadate-treated cells (Lanier, L. L., 1998, NK
cell receptors. Annu. Rev. Inununol., 16:359);
were subjected to immunoprecipitation with anti-TREM-1 (21C7) mAb, anti-TREM-2 3() (29E3) mAb anti-signal-regulatory protein (SIR?) (Dietrich et al., J.
Immutzol., 164:9, 2000), mAb as a positive control, or control IgG1 (anti-MHC class I mAb). The precipitates were analyzed by Western blot either with anti-phosphotyrosine PY20-HRP (Transduction Laboratories, Lexington, KY) under reducing and nonreducing conditions (see Fig. 12 and Fig. 28) or with anti-DAP12 rabbit antiserum followed by Human/Mouse-adsorbed anti-Rabbit IgG-FIRP (SBA) under reducing condition (see Fig. 13 and Fig. 29).

, 6.11 Chemotaxis Assay Monocyte-derived DCs were stimulated for 24 hours with LPS (11.ig/m1) or with F(ab)'2 9E2 (anti-TREM-1, IgGl, ic) or F(ab)'2 29E3 (20Ag/m1) coated on plastic plates. These cells (5 x 105 cells/100 Al/well in IMDM/0.5% BSA) were incubated for 1 hour at 37 C in new media and subsequently loaded into collagen-coated transwells (Costar, 3 gm pore filter), which were placed to 24-well plates containing 450 Al medium supplemented with 100 ng/ml ELC or MIP-313. After an incubation period of 4 hours at 37 C, cells that had migrated to the lower chamber were collected and counted on a FACSCalibur (constant time acquisition). In blocking experiments, cells were preincubated with ELC (100 ng/ml) or MIP-313 (100 ng/ml) for 1 hour, or anti-CCR7 mAb (1 jig/ml) was added to the transwell (see Fig. 37).
6.12 Detection of Apoptosis As shown in Fig. 34, monocyte-derived DCs were stimulated with GM-CSF/IL-4 (closed squares), plastic-bound F(a1302 (open circles) or control F(a13')2 (closed circles) for the indicated time periods and determination of DNA fragmentation was performed as described previously (Nicoletti, I., etal., 1991, J. 1111MU1701.
Methods 139:271-279). In experiments where kinase inhibitors (PD98059 (501.tg/m1) or LY294002 (10p.g/m1)) or serine protease inhibitor (TPCK (15 jig/m1)) were used, the inhibitors were added 60 min before stimulation (see Fig. 35) and apoptotic cell death was determined after 8 days by measurement of DNA fragmentation. All inhibitors had no effect on cell viability or the rate of constitutive apoptosis at the indicated concentrations.
6.13 Internalization Assays Monocyte-derived DCs were incubated in RPMI-10%FCS with 21.1g/m1 of 29E3 (anti-TREM-2 mAb; whole IgGl, Fab or F(a131)2) or 1F1 1 (anti-MHC class I
mAb;
whole IgG I) for 15, 30, 60 and 120 minutes at 37 C (see Fig. 38). One aliquot was kept at 4 C for 2 hours in the presence of antibodies to determine the initial levels of TREM-2 expression. Activation was stopped by washing cells twice with ice-cold PBS.
Residual surface levels of receptors were measured by FACS after stimulated cells were fixed with 3% paraformaldehyde (PFA) in PBS and staining with PE-conjugated goat anti-mouse IgG
antibody (PharMingen) (extracellular receptor levels). Intracellular receptor levels were determined by stripping the cell surface with PBS containing 150 mM13-mercaptoethanol and 5M NaCI for 5 min, followed by fixation, pemicabilization with PBS
containing 0.1%
Saponin and 2% FCS and staining with PE-conjugated goat anti-mouse IgG
antibody. To determine the total amount of the receptor amount and to assess the degree of mAb shedding, stimulated cells were stained for external and internal receptor expression after fixation and permeabilization. When biotinylated Fab or F(ab')2 fragments of 29E3 were used (5 g/m1), the bound antibody was detected using PE-conjugated Streptavidine (Phan-ningen). To prevent the progression of receptor internalization, cells were kept on ice during the whole procedure.
6.14 Antigen Presentation Assay Irradiated (3000 rad) 2.5 x 104 of DCs were cocultured with 5 x 104 cells of the VIP13 T cell clone in 96-well flat-bottom microplates in the presence of serial dilutions of whole IgG1 mAbs or F(ab')2 fragments. Monoclonal antibodies used as whole IgG1 molecules are ZM3.8 (anti-ILT3, IgG1 oc); 9E2 (anti-TREM-1, IgGl, x); 29E3 (anti-TREM-1, IgGl, x); and ICRF44.(anti-CD11b/Mac-1, IgG I , K, Pharmingen), and F(ab')2 fragments used in the assay were F(ab')2 9E2 and F(ab'), 29E3. After 72 hours, the cultures were pulsed with [31-I]thymidine (1 Ci/well; specific activity: 5 Ci/mmol), and the radioactivity incorporated was measured after additional 16 hours. The data were plotted against the concentration of mAbs determined by ELISA using a purified mouse IgGl, K or F(ab')2 IgG1,1( as a standard (see Fig. 39).
6.15 Protection of Mice from Endotoxemia 6.15.1 LPS-induced endotoxemia Female C57BL/6 mice (8-10 weeks, 19-22 g) were randomly grouped (5-10 mice per group) and injected intraperitoneally (i.p.) with LPS from E. coli 055:B5 (Sigma) (an LD100, 20 mg per gram body weight, for Figures 20, 21(a) and (c); or different amounts, for Figure 21(b)). Purified huTREM-1-IgGI, mTREM-I -IgGl, huIgG1 (Sigma), heat-inactivated mTREM-I-IgGl, or ILT3-IgG1 (Cella, M. etal., 1997, A novel inhibitory receptor (ILT3) expressed on monocytes, macrophages, and dendritic cells involved in antigen processing. 1 Exp. Med. 185:1743-51), at 500 g/mouse, was administrated, i.p., I, 2, 4, and 6 hours after (see Figure 21(c)) or 1 hour prior (see Figures 20, 21(a) and 21(b)) to LPS. Treated mice were monitored 4-6 times a day for at least 10 days.
6.15.2 E. coliperitonitis model E colt peritonitis was induced in mice as described previously (Appelmelk, B. J. et al., 1986, Use of mucin and hemoglobin in experimental murine gram-negative bacteremia enhances the immunoprotective action of antibodies reactive with the lipopolysaccharide core region. Anionic Van Lecuwenhoek 52:537-42). Briefly, mice (female, 8-10 weeks, 19-22 g) were weighed and randomly distributed into groups of 5-15 animals of equal body weight. Mice were injected i.p. with 500 mg of mTREM-1-IgG1 or control huIgG1 prior to i.p. administration of 500 gl of a suspension of E. col"
0111:B4 (1.6-2.1 x 106 CFU per mouse).
6.15.3 Cecal ligation and puncture (CLPI
CLP was performed as described previously (Echtenacher, 13. et al., 1990, Requirement of endogenous tumor necrosis factor/cachectin for recovery from experimental peritonitis. J. Immunol. 145:3762-6; Calandra, T. et al., 2000) Protection from septic shock by neutralization of macrophage migration inhibitory factor. Nat. Med. 6:164-70). Briefly, C57BL/6 mice (female, 8-10 weeks, 19-22 g) were anaesthetized by intraperitoneal administration of 75 mg/kg Ketanest (Parke Davies & Company, Munich, Germany) and =
16mg/kg Rompun (Bayer AG, Leverkusen, Germany) in 0.2 ml sterile pyrogen-free saline (B. Braun Melsungen AG, Melsungen, Germany). The caecum was exposed through a 1.0-1.5 cm abdominal midline incision and subjected to a 50-80% ligation of the distal half followed by a single puncture with a G23 needle. A small amount of stool was expelled from the punctures to ensure patency. The caecum was replaced into the peritoneal cavity TM
and the abdominal incision closed in layers with 5/0 Prolend thread (Ethicon, Norderstedt, Germany). Five-hundred (500) ill sterile saline containing 500 mg of mTREM-1-IgGl, 500 mg of huIgGI,K (Sigma) or 100 fig TNF-RI-IgG1 (Pharmingen) (together with 400 jig hulgGI,K (Sigma) was injected intraperitoneally immediately after CL?. The CLP
was performed blinded to the identity of the treatment group. Survival after CLP
was assessed 4-6 times a day for at least 7 days.
6.16 Analysis of Blood and Lavage Fluids Blood (250 1) was collected from the tail vein of mice into a Serum Separator Tube (Becton Dickinson) at different time points after induction of LPS-induced endotoxemia in the presence of TREM-I -IgG1 or control IgGl. Quantification of murine TNF-a and IL-I f3 in the serum was determined using cytokine-specific ELISAs according to the manufacturer's protocol (R&D Systems, Minneapolis, MI).
Peritoneal lavage (PL) cells were harvested at different time points after LPS
administration in the presence of TREM-1-IgG1 or control IgGI. Total cell numbers were determined on a Coulter counter and differential counts were performed according to standard morphological criteria on cytospin preparations stained with Giemsa &
May-Gruenwald solution (Sigma). A minimum of 200 cells were counted per field, with 3 fields per sample for PL. Sec Figures 22(c) and (d).

Four-color analysis of peritoneal leucocytes from LPS-treated C-57BL/6 mice (Fig. 19(d)) compared to control animals (Fig. 19(c)) was conducted using anti-TREM-1, anti-LY-6G (PharMingen) and anti-Mac-1 (PharMingen) monoclonal antibodies conjugated with Cy5, Phycoerythrin (PE) and Fluorescein isothiocyanate (FITC), respectively, and biotinylated anti-F4/80 followed by streptavidine-CyChrome (Pharmingen).
B. RESULTS
TREMs are novel transmembrane proteins of the Ig-SF
As shown in Fig. 1(a), the amino acid sequence of TREM-1 begins with a hydrophobic signal peptide followed by an extracellular region composed of a single Ig-SF
domain containing three potential N-glycosylation sites. The length of the Ig-type fold and the characteristic pattern Asp-Xaa-Gly-Xaa-Tyr-Xaa-Cys in the region leading to the f3-strand F indicate that the Ig-type fold is of the V-type. The putative transmembrane domain contains a charged lysine residue and is followed by a cytoplasmic tail of 5 amino acids with no signaling motifs. Similar transmembrane and cytoplasmic domains are present in activating NK cell receptors which pair with the trans-membrane adapter protein DAP12 (Lanier, L. L., 1998, NK cell receptors. Annu. Rev. Immunot, 16:359). A cDNA
containing the entire open reading frame was amplified by RT-PCR from monocytes and neutrophils, but not from lymphocytes or other cell types (data not shown).
Therefore, this molecule was designated as TREM-1. The GenBank EST database was then searched with TREM-1 polypeptide, and a novel cDNA encoding a TREM-1-homologue was identified.
and designated as TREM-2 (Fig. 1(b)), which has very similar structure to that of TREM-1.
The alignment of TREM-1 , TREM-2, and NKp44 extracellular domains revealed ¨20%
identity (data not shown). Analysis of somatic cell hybrids containing different human chromosomes demonstrated that the genes encoding TREMs map on human chromosome 6, as does the NKp44 gene (data not shown).

TREM-1 is selectively expressed on blood neutrophils and monocytes and is up-regulated by bacterial and fungal stimuli In three-color FACS analysis of whole blood leukocytes, high side scatter cells correspond to TREM-1' neutrophils. Low side scatter cells include CD14high/HLA-DR+ cells (monocytes), CD I 4dim/HLA-DR' (monocytes), CD14-/HLA-DR cells (which include B cells and dendritic cells or DCs), and CD141HLA-DR- cells (mostly lymphocytes). In peripheral blood of different donors, 2IC7 mAb stained neutrophils and, to a less extent, CD14Ingh monocytes (Figs. 5(c) and 5(d)). CD14dim monocytes, DCs or lymphocytes were TREM-1 negative (Figs. S(e), 5(1), and 5(g)). The expression of TREM-_ was further investigated during differentiation of CD14+ monocytes into either DCs or macrophages in the ptesence of GM-CSF/IL-4 or M-CSF, respectively_ TREM-I was completely down-regulated on these cells after 3 days of culture (data not shown).
Stimulation of DCs with LPS, heat-inactivated Gram-positive bacteria, Gram-negative bacteria, or fungi did not induce TREM-1 expression (data not shown). In striking contrast, these stimuli induced strong up-regulation of TREM-1 on neutrophils and monocytes (for LPS stimulation, see Fig. 6; for other stimulants, data not shown). This selective expression of TREM-1 on neutrophils (Fig. 6(b)) and monocytes (Fig. 6(a)) and its induction by pathogens indicate its role in acute inflammatory responses.
TRE111-1 is a ¨30-kDa glycoprotein associated with DAP12 Biochemical analysis of TREM-1 immunoprecipitated from surface-biotinylated monocytes revealed that TREM-I is a glycoprotein of-30 kDa, which is reduced to 26 kDa after N-deglycosylation, in agreement with the predicted molecular mass of TREM-1 (Fig. 11). Because TREM-1 lacks known signaling motifs in the cytoplasmic domain, it should associate with a separate signal transduction subunit to mediate activating signals. Adapter molecules, such as DAP12, are tyrosine phosphorylated upon cell treatment with the phosphatase-inhibitor pervanadate (Lanier, L. L., 1998, NK
cell receptors. Annu. Rev. Immunol., 16:359). Indeed, anti-phosphotyrosine blotting of TREM-1 immunoprecipitates from pervanadate-stimulated monocytes revealed a phosphorylated protein of-12 kDa and ¨24 kDa under reducing and nonreducing conditions, respectively (Fig. 12). An identical pattern was observed after immunoprecipitation of SIRPP1, which is associated with DAPI2 (Dietrich et al., 2000, Signal-regulatory protein 01 is a DAP12-associated activating receptor expressed in myeloid cells, J. Inummol., 164:9).
Indeed, immunoblotting of TREM-1 immunoprecipitates with anti-DAP12 demonstrated that TREM-I associates with (Fig. 13).
TREM-1 triggers release of pro-inflammatory chemokines and cytokines, as well as increased surface expression of cell activation markers In neutrophils, cross-linking of TREM-1 induced secretion of IL-8 and release of MPO (Fig. 7, A and B). This latter release was strongly potentiated following priming of neutrophils with LPS (Fig. 7C). In monocytes, cross-linking of TREM-generated release of large amounts of IL-8 as well as MCP-1 and TNF-a (Fig. 7, D-F).
TNF-a and MCP-1 secretion was strongly up-regulated by LPS-mediated priming (Fig. 7, G

and H), further demonstrating the importance of bacterial costimuli for TREM-1-mediated activation. In control experiments, neutrophils and monocytes were stimulated with isotype-matched Abs which either bind (such as anti-MHC class I mAbs) or do not bind (such as an anti-TNP rnAb) cells. In both cases, secretion of cytokines, chemokines, and MPO was 5- to 50-fold lower than that induced via TREM-1 (Fig. 7 and data not shown).
Thus, activation of neutrophils and monocytes triggered by anti-TREM-1 mAb is not due to engagement of Fc receptors. Secretion of cytokines important for the adaptive immune response, such as IL-6, IL-10, IL-12, or for surveillance against viral infections, such as type I IFN, were not significantly increased by engagement of TREM-1 (data not shown).
The rapid migration of neutrophils and monocytes from the blood stream to the inflammatory site requires adhesion to endothelium and extracellular matrix proteins (Springer, T.A., 1994, Traffic signals for lymphocyte recirculation and leukocyte emigration: the multistep paradigm, Cell, 76:301). Therefore, whether engagement of TREM-1 stimulates upregulation of adhesion molecules was tested. As shown in Table I, cell surface expression of CD29, CD11c, and CD49e, and to a lesser extent CD11b, CD49d, and CD18 were increased on both neutrophils and monocytes. Thus, TREM-1 may increase cellular adhesion to fibronectin, fibrinogen, and VCAM by upregulating CD11b/CD18 (Mac-1), CD29/CD49d, and CD29/CD49e heterodimers, respectively. In addition, TREM-1 stimulation led to a strong upregulation of the costimulatory molecules CD40, CD86 (B7-2), and CD54 (ICAM-1), as well as of CD83 and CD32 (FcRII) on monocytes.
Thus, TREM-1 is not only capable of increasing adhesion of myeloid cells to endothelium and extracellular matrix molecules but also can prepare monocytes for costimulation of other cells recruited to the inflammatory lesions.
Table 1 TREM-1-dependent regulation of cell surface activation markers' Stimulation for 24 h Neutrophils Monocytes Surface Marker Anti-MHC Anti-TREM- Anti-MHC Anti-TREM-1 class I 1 class I
CD40 23.9 254.1 CD80/137.1 0.6 0.1 CD86/B7.2 32.6 521.5 CD54/ICAM I 10.9 35.6 97 97.5 , -CD1 lb 0.4 27.9 234.9 _ 256.8 CDI lc 75.3 85.7 175.6 385.5 CD18 54.8 76.9 198.8 211.9 CD49d 21.8 30.2 0.1 4.8 CD49e 76.1 91.9 14.9 46.5 CD29 2.7 14.7 23.2 76.9 CD32/FcR11 86.2 100.2 72.1 114 CD83 0.9 44.6 Monocytes or neutrophils cultured for 24 h in plates either coated with anti-TREM-1 or control IgG1 (anti-MHC class I mAb). Cells were then analyzed by FACS for the indicated cell surface molecules. Numerical values indicate specific mean fluorescence intensity (MFI) after subtraction of the fluorescence detected with an isotype-matched control. The shown data are representative for seven experiments.
Stimulation of TREM-I induces calcium nzobilization and tyrosine phosphorylation Activation of neutrophils and monocytes is often accompanied by a number of intracellular changes. Indeed, ligation of TREM-1 with the mAb 21C7 elicited a rapid rise in intracellular Ca2+ concentration (Fig. 8(b) and (c)). Ca2 mobilization occurred even in the absence of a cross-linking Ab (Fig. 8(b)). In addition, cross-linking of TREM-1 stimulated tyrosine phosphorylation of several proteins with apparent molecular masses of -40, -60, -70, and -100 kDa as indicated with arrows in Fig. 9. The observed -40-1cDa tyrosine phosphorylated proteins correspond to mitogen activated protein kinases, as demonstrated by anti-phospho-ERK1/2 immunoblotting (Fig. 10(a)). Precipitation of tyrosine phosphorylated proteins and immunoblotting with an anti-PLC-y Ab, revealed that the observed -100-kDa phosphoprotein corresponds to PLC-7 (Fig. 10(b)), thus explaining the observed Ca24 influx.
Human TREM-1 expression on nentrophils and monocytes is strongly upregulated by bacterial and fungal stimuli.
As shown in Fig. 14A, TREM- I expression was strongly upregulated by gram-positive and gram-negative bacteria, such as Staphylococcus aureus and Pseudomonas aeruginosa, respectively, but not by mycobacteria, such as Bacillus of Cahnette-Guerin (BCG). TREM-I expression was also increased by incubating monocytes and granulocytes with gram-positive and gram-negative bacterial cell wall components, such as lipoteichoic acid (LTA) and lipopolysaccharide (LPS), whereas incubation with mycobacterial mycolic acid had no effect (Fig. I48). Proinflamrnatory cytokines, such as TNF-a and IL-113, produced a moderate increase of TREM-1 expression, while 1L-

10, TGF-13 (Fig. 15) and dexamethasone (data not shown) which mediate anti-inflammatory responses, completely abolished TREM-1 expression. These results suggested that TREM-1 is upregulated under inflammatory conditions, especially those caused by gram-positive and gram-negative bacteria. TREM-1 upregulation was paralleled with an increased capacity of triggering inflammatory responses in vitro. As shown in Fig. 16, secretion of TNF-a and IL-113 induced by cross-linking of TREM-1 on monocytes was strongly potentiated bypriming of monocytes with LPS.
Human TEEM-1 is selectively expressed in bacterial infections TREM-1-expression in in vivo was determined in tissue specimens derived from acute or granulomatous inflammatory lesions caused by either bacterial, fungal or non-microbial agents. As shown in Fig. 17, TREM-1 expression level was extremely strong in neutrophils associated with suppurative lesions of the skin, such as folliculitis and impetigo, caused by Staphylococcus aureus (Fig. 17(a) and (c), respectively). Obvious expression was also observed in suppurative granulomatous lymphadenitis caused by Bartonella henselae and Aspergillus fumigatus (Fig. 17(e) and (g), respectively). In the latter, TREM-1 was expressed not only in neutrophils, but also in epithelioid and multinucleated giant cells surrounding the suppurative granulomas (Fig.
17(g)). In contrast, TREM-1 positivity was either weak and focal or totally absent in granulomatous lymphadenitis caused by Mycobacterium tuberculosis as well as in sarcoid and foreign bodies granulomas (data not shown). In all cases showing TREM-1 positive inflammatory infiltrates, the reactivity was mostly confined to the cells within the inflammation, and was absent from the surrounding tissues.
Infections by gram-positive and gram-negative bacteria and by certain fungi are characterized by the recruitment of large numbers of neutrophils, which collect in the inflammatory site to form an exudate known as pus. Thus, one could argue that the strong expression of TREM-1 in bacterial and fungal infections is simply due to the massive infiltration of neutrophils. However, TREM-1 was poorly expressed in neutrophilic infiltrates found in non-bacterial inflammatory reactions. As shown in Fig.
18(a), psoriasis is characterized by a prominent infiltration of neutrophils which form microabscesses within the hyperproliferative epidermis. However, TREM-1 was weakly expressed in psoriasis (Fig. 18(b)). Similarly, ulcerative colitis, vasculitis caused by immune complexes and lipoid pneumonias expressed very low levels of TREM-1 despite a considerable infiltration of neutrophils and monocytes (Fig. 18(c)-(f) and data not shown)-Together, these results are consistent with a predominant role of TREM-1 in acute and granulomatous inflammations caused by bacterial and fungal products.
Soluble TREM-I protects mice from LPS-induced lethal endotoxemia Mouse TREM- 1, which is 90% similar to human TREM-1, is expressed on murine granulocytes and monocytes/macrophages isolated from blood.
Importantly, mouse TREM-1 expression is upregulated in peritoneal granulocytes and macrophages after intraperitoneal injection of LPS. See Fig. 19(d). Thus, human and murine TREM-1 have similar cell surface expression pattern and regulation. If TREM-1 promotes host inflammatory responses to bacterial and fungal products in vivo, inhibition of using soluble TREM-1 as a receptor decoy would be predicted to reduce such responses.
To test this hypothesis, LPS-induced septic shock in mice was chosen as a model of acute inflammation. In this model, intraperitoneal injection of LPS leads to tissue damage, hemodynamic changes, multiple organ failure and death. This process is caused by the massive release of proinflammatory mediators, such as TNF-a, IL-113, macrophage migration inhibitory factor (M1F) and high mobility group-1 (HMG-I) protein (Wang, H. et al., 1999, HMG-1 as a late mediator of endotoxin lethality in mice. Science 285:248-51). A
murine TREM-I -human IgG1 fusion protein (mTREM-I-IgG1) was injected in the peritoneal cavity (500 jig fusion protein per animal) I hour before the induction of endotoxemia by LPS. Lethality was monitored over time by comparing to animals which had received control injections of heat-inactivated mTREM-1-IgG1 (500 jig/animal), human IgG1 (500 g/animal) or control-IgG1 fusion protein (500 jig/animal) prior to LPS
administration. As shown in Fig. 21(a), 76% of the mice treated with mTREM-1-IgG1 (open circles) was protected from a lethal dose of LPS (400 pig/mouse) as compared to 7%
of mice that received control proteins (IgGl, ILT3-IgGI, or heat-inactivated mTREM-1-IgG1). The heat-inactivated mTREM-1-IgGI was included as a control to exclude a possibility that the mTREM-1-IgG I preparation was contaminated by LPS which could tolerize the mice to the subsequent injection of LPS, thus possibly explaining the observed protective effect. Heat inactivation of the inTREM-1-IgGI denatures the soluble protein without affecting potential contaminating endotoxins. As shown in Fig. 21(a) (closed triangles), the heat-inactivated preparation lost completely its protective capacity against LPS-induced endotoxemia, demonstrating lack of LPS-induced tolerization by mTREM-1-IgGI. All susceptible mice succumbed to LPS within the first 24 hours, showing severe signs of endotoxemia, such as shivering and lethargy. In contrast, TREM-1-IgGl-treated mice showed mild symptoms during the first few hours after LPS injection, recovered completely within day 4 after LPS injection and survived showing no signs of sickness or physical limitations_ To precisely quantify the protection provided by mTREM-1-IgGl, groups of mice pre-treated with mTREM-1-IgG1 or huIgG1 were challenged with various doses of LPS. The LD50 of LPS in animals treated with mTREM-1-IgGI (LD50= 621 fig) was significantly higher than the LD50 in control animals (LD50 = 467 fig) (Fig.
21(b)).
Since clinical diagnosis and treatment of septic shock usually occurs hours after the onset of an infection, it was important to determine whether mTREM-1-IgG1 could protect mice from LPS-induced lethal shock when injected after administration of LPS.
Accordingly, 500 fig of mTREM-1-IgG1 were injected into mice at 1 hour, 2 hours, 4 hours and 6 hours after LPS injection and monitored lethality as compared to animals treated with control proteins. See Fig. 21(c). Remarkably, mTREM-1-IgG1 conferred 80%
protection against endotoxic shock when applied 1 hour after LPS injection. Partial protection was also observed after 2 and 4 hours, whereas no protection occurred after 6 hours. Thus, soluble TREM-1 is effective even when injected after the outbreak of endotoxemia.
To explore the serological and cellular mechanisms by which TREM-1-1gG1 conferred protection to the mice, blood samples from mice pretreated with TREM-1-IgG1 and control animals were tested at different time points after LPS
administration to determine TN F-a and IL-113 serum levels by ELISA. In both groups, TNF-a levels peaked at 1-2 hours after LPS injection, while IL-113 levels peaked at approximately 6 hours after LPS-injection. The survival benefit obtained with TREM-1-IgG1 was associated with a significant reduction of the plasma concentrations of both TNF-a and IL-113 (Figs. 22(a) and (b), respectively). The cellular composition of the peritoneal lavage at various time points after injection of LPS in mTREM-1-IgGl-pretreated animals and controls was also studied.
A significant reduction in the total cell number of neutrophils and monocytes/macrophages infiltrating the peritoneum 6-8 hours after LPS injection was observed in mTREM-1-IgG1-pretreated animals as compared to controls (see Figs. 22(c) and 22(d)).
Injection of mTREM-l-IgGI in normal mice did not affect the levels of circulating leukocytes.
Furthermore, mTREM-1-IgG1 was effective against endotoxemia even when the IgGI-Fc portion of the fusion protein was mutated to inhibit Fc receptor binding and complement fixation (data not shown). Thus, inhibition of TREM-1-mediated responses is sufficient to lower systemic levels of TNF-a and 1L-l1 and reduce cellular infiltrates at the site of inflammation below levels that are lethal for the host, without causing leukopenia.
Remarkably, human TREM-1-IgGI fusion protein also provided protection against LPS-induced endotoxemia in mice suggesting a substantial functional identity of TREM-1s between mouse and human (Fig. 20).

=
mTREM-1 is protective in bacterial peritonitis Experimental endotoxic shock reproduces human sepsis only in part, since it does not involve the replication and dissemination of bacteria. In these conditions, a complete block of TREM-1 signalling could be deleterious by impairing the capacity of the immune system to fight infections, as previously observed for anti-TNF-a treatments (Echtenacher, B. et al., 1990, Requirement of endogenous tumor necrosis factor/cachectin for recovery from experimental peritonitis. J. Immunol. 145:3762-6;
Echtenacher, B. et al., 1996, Critical protective role of mast cells in a model of acute septic peritonitis. Nature 381:75-7; Malaviya, R. et al., 1996, Mast cell modulation of neutrophil influx and bacterial clearance at sites of infection through TNF-alpha. Nature 381:77-80; Rothe, J.
et al., 1993, Mice lacking the tumour necrosis factor receptor 1 are resistant to TNF-mediated toxicity but highly susceptible to infection by Listeria monocytogenes. Nature 364:798-802; Pfeffer, K. et al., 1993, Mice deficient for the 55 kd tumor necrosis factor receptor are resistant to endotoxic shock, yet succumb to L. monocytogenes infection. Cell 73:457-67;
Peschon, J. J.
et al., 1998, TNF receptor-deficient mice reveal divergent roles for p55 and p75 in several models of inflammation. J. 1171171141701. 160:943-52; Eskandari, M. K. etal., 1992, Anti-tumor necrosis factor antibody therapy fails to prevent lethality after cecal ligation and puncture or endotoxemia. I Immunol. 148:2724-30). Accordingly, two models of microbial peritonitis and sepsis caused by intraperitoneal administration of Escherichia coli or by cecal ligation and puncture (CLP) were studied to see whether mTREM-1-IgG1 protects against septic shock. As shown in Figure 23, injection of mTR_EM-1-IgG1 conferred significant protection against lethal E. coil peritonitis (a) and CLP-induced septic shock (b) compared to control huIgGl. On the other hand, in the CLP model, treatment with TNF-a receptor I-IgG1 (TNF-RI-IgG1) caused accelerated and complete death of all animals (Fig.
23(b)).
Thus, mTREM-1-IgG1 reduces inflammatory responses but still allows sufficient control of the bacterial infection.

TREM-2 is selectively expressed on immature DCs and IL-4-stimulated monocytes As shown in Fig. 25, three-color FACS analysis for TREM-2 expression on monocytes was conducted using 29E3 mAb which is specific for TREM-2 (see Fig.
24).
Monocytes were stimulated with M-CSF (a), GM-CSF (c), IL-4 (d), or GM-CSF + IL-4 (b) for 36 hours. TREM-2 expression was strongly upregulated after stimulation of monocytes with either IL-4 alone or GM-CSF + IL-4. Furthermore, in three-color FACS
analysis for TREM-2 and CD83 (DC surface marker) expression on monocyte-derived DCs that are stimulated with LPS, CD4OL, TNF-a, or medium for 36 hours (Fig. 26), TREM-2 was rapidly downregulated upon maturation of DCs, demonstrating that TREM-2,is selectively expressed on immature DCs. Also see Fig. 30.
TREM-2 is a ¨40-kDa glycoprotein associated with the adaptor protein DAP12 TREM-2 immunoprecipitated from surface-biotinylated monocyte-derived DCs showed that TREM-2 is a glycoprotein of ¨40 kDa, which is reduced to ¨26 kDa after N-deglycosylation (Fig. 27). Similar to TREM-1, TREM-2 also lacks signaling motifs in the cytoplasmic domain and requires a separate signal transduction subunit to mediate activating signals. As shown in Fig. 28, when the immunoprecipitates of pervanadate-treated monocyte-derived DCs by anti-TREM-2 mAb was subjected to Western blot analysis using anti-phosphotyrosine blot under reducing and non-reducing conditions, a tyrosine-phosphorylated protein was observed. This protein was also detected by anti-DAP12 blot analysis (Fig. 29), demonstrating that TREM-2 also associates with adaptor molecule.
TREM-2 does not trigger secretion of cytokines and chemokines by DCs but upregulates certain cell surface activation markers To study whether stimulation of TREM-2 on DCs triggers the secretion of proinflammatory cytokines and chemokines, DCs were stimulated by culturing for 48 hours in the plates coated with either F(ab`)2 control mAb (anti-TREM-1), F(ab')2 anti-TREM-2 mAb, or LPS and the culture supernatant were analyzed by ELISA for secretion of various mediators. As shown in Table II below, TREM-2 stimulation did not trigger secretion of cytokines and chemokines by DCs. The data are representative of 5 independent experiments.
Table 11 Secretion of cytokines and chemokines by stimulation of TREM-2 on DCs Cytokines/
Chemokines F(ab1)2 anti-TREM-1 F(ab1)2 anti-TREM-2 LPS
(r1g/m1) IL-)a N.D.a N.D. 0.135 0.026 IL-10 0.027 0.012 N.D. 0.162 0_09 TNF-a 0.042 0.005 N.D. 4.015 0.078 IL-18 N.D. N.D. 2.56 1.31 1L-6 N.D. N.D. 16.7 5.43 IL-I0 N.D. N.D. 2.63 0.45 TGF-31 N.D. N.D. N.D.
IL-12p40 N.D. N.D. 3.48+
1.25 \IL-12p70 N.D. N.D. 1.45 0.09 IL-13 N.D. N.D. N.D.
IL-15 N.D. N.D. N.D.
MCP-1 2.018 0.875 0.449 0.067 98.18 35.86 IL-8 1.23 0.451 0.023 0.01 124.76 23.91 a Not detectable.
TREM-2-dependent regulation of cell surface activation markers was also studied. DCs were stimulated as described above and analyzed by FACS for various cell surface molecules. See Table III below. Numerical values indicate specific mean fluorescence intensity (MFI) after subtraction of the fluorescence detected with an isotype-matched control. The data are representative of 5 independent experiments. The surface markers which are especially upregulated by TREM-2 stimulation compared to controls (treated with anti-TREM-1 mAb F(abl), are indicated in bold face.
Table III
TREM-2-dependent regulation of cell surface activation markers Surface marker F(ab')2 anti-TREM-1 F(ab')2 anti-TREM-2 LPS
MHC class 1 67.8 65.3 107.1 MHC class II 89.12 168.65 214.67 CD40 171.35 398.6 435.89 _ CD86/B7.2 14.04 287.91 683.56 CD83 3.34 3.23 26.7 CD1a 106.76 134.9 87.54 CCR5 12.95 13.56 3.12 CCR6 3.68 3.45 4.01 CCR7 6.82 19.98 5.45 CXCR4 5.13 4.56 17.8 CD I la/aL 10.92 6.78 13.72 CD11b/aM 53.9 65.7 23.1 CD 1 1c/aX 91.1 65.7 123.5 CD29/131 38.22 37.56 37.5 CD31/PECAM-1 3.52 16.92 3.21 CD41/a1113 4.54 4.67 4.39 CD54/ICAM-1 56.87 54.78 271.45 CD61/133 4.95 5.03 4.21 CD103/aE 3.63 3.96 3.26 Mannose-R 81.8 82.9 30.9 CD32 17.21 16.78 2.34 CD89/FcaR 4.54 4.75 4.96 CD35/CR I 3.94 423 3.67 M-CSF-R 14.6 423 5.21 GM-CSF-R 15.6 13.7 13.5 TREM-2 ligation on monocyte-derived DCs induces calcium mobilization and tyrosine phosphorylation and prolongs DC survival by an Erk-dependent pathway As shown in Fig. 31, cross-linking of TREM-2 induced intracellular Ca2+-mobilization in monocyhte-derived DCs. Monovalent engagement of TREM-2 by Fab 29E3ll'0"" was sufficient for induction of Ca2'-flux only in the presence of cross-linking Streptavidine (data not shown). Furthermore, cross-linking of TREM-2 stimulated tyrosine phosphorylation of several proteins as indicated with arrows in Fig. 32.
Again, -40-kDa tyrosine phosphorylated proteins correspond to mitogen activated protein kinases, as demonstrated by anti-phospho-ERK1/2 immunoblotting (Fig. 33). Monocyte-derived DCs stimulated with GM-CSF + IL-4 or F(abi), 29E3 for various time periods showed resistance to apoptosis (Fig. 34) and the prolonged survival of these cells seem to be mediated by an Erk-depcndent pathway (Fig. 35).

Stimulation of TREM-2 induces CCR7 expression and DC chemotaxis to EL and MIP-_ FACS analysis of DCs which were stimulated with anti-TREM-2 mAb F(ab')2 showed that TREM-2 stimulation induced rapid upregulation of CCR7 expression by DCs (Fig. 36). Furthermore, chemotaxis assays showed that DCs stimulated with anti-TREM-2 mAb F(ab')2 exhibited strong chemotaxis towards ELC and MIP-313 and this chemotaxis occurred via a CCR7-dependent pathway because the presence of anti-mAb inhibited the migration of DCs towards ELC and MIP-313 (Fig. 37).
TREM-2 is internalized upon ligation and functions as an antigen-capturing molecule in vitro Monocyte-derived DCs were stimulated with anti-TREM-2 mAb, its F(ab')2 or Fab fragments and the amount of total, extracellular and intracellular TREM-2 were measured by FACS analysis using goat anti-mouse IgG-PE as a second antibody.
As shown in Fig. 38, antibody-bound TREM-2 was quickly internalized and the degree of internalization was higher with divalent antibodies (i.e., whole anti-TREM-2 mAb and its F(ab')2 fragments) than monovalent antibody (Fab fragments). Presentation of anti-TREM-2 mAb to a T cell clone specific for mouse IgG1 by irradiated DCs was also studied based on the [31-I]thymidine uptake by the T cells (Fig. 39) cocultured with DCs in the presence of serially diluted mAbs or their F(ab')2 fragments. Anti-TREM-2 mAb and its F(ab')2 were presented --100-fold more efficiently than F(ab')2 of control mAb.
C. SUMMARY
These results presented here demonstrate that TREM-1 mediates a novel proinflammatory pathway in granulocytes and monocytes. Such a pathway is particularly important in regulating inflammatory responses to bacterial and fungal infections. Namely, human TREM-1 is upregulated in the presence of heat-inactivated bacteria or bacterial cell wall components in vitro and, most importantly, in bacterial infections of the skin and lymph nodes in vivo. In addition, mouse TREM-1 significantly contributes to the pathogenesis of LPS-induced shock, as demonstrated by prevention of clinical, cellular and serological manifestations of endotoxemia in the presence of an inhibitor of TREM-1.
Thus, the present inventors propose a role of TREM-1 as an amplifier of inflammatory responses triggered by pattern recognition receptors (PRRs). In an early phase of a bacterial infection, neutrophils and monocytes are rapidly alerted through the engagement of PRRs by bacterial products_ This leads to an initial release of proinflammatory cytokines which is required to launch an inflammatory response. At the same time, bacterial products induce upregulation of TREM-1, which, upon engagement, activates DAP12-signaling pathways (Lanier, L. L. et al., 1998, Immunoreceptor DAP12 bearing a tyrosine-based activation motif is involved in activating NK cells. Nature 391:703-7), which amplify inflammatory responses. The present inventors have shown that ligation of TREM-1 leads to sustained secretion of proinflammatory cytokines (TNF-a and IL-113) and chemokines (IL-8 and MCP-1), and may also result in prolonged survival of neutrophils and monocytes at the inflammatory site. Upon clearance of the pathogenic stimuli, TREM-1 is downregulated, contributing to the reduction of the inflammation and the enhancement of tissue repair. It will be important to define the ligand that engages TREM-1 during inflammatory responses.
TREM-I ligand could be a soluble mediator released by cells following recognition of bacterial products by PRRs and intracellular signaling. Alternatively, TREM-1 ligand could be a cell surface molecule that is upregulated during bacterial infections to alert granulocytes and monocytes to elicit a strong inflammatory response. Cell surface molecules with an hypothetical "alert" function have been recently identified on epithelial cells (Bauer S, Groh V, Wu J, Steinle A, Phillips JH, Lanier LL, and Spies T., 1999, Activation of NK cells and T cells by NKG2D, a receptor for stress-inducible MICA.
Science 285:727-9; Katsuura M, Shimizu Y, Akiba K, Kanazawa C, Mitsui T, Sendo D, Kawakami T, Hayasaka K, and Yokoyama S., 1998, CD48 expression on leukocytes in infectious diseases: flow cytometric analysis of surface antigen. Acta Paecliatr Jpn. 40:580-5). These molecules, called M1C and CD48, are over-expressed during heat shock, stress and viral infections and detected by the natural killer (NK) cell activating receptors NKG2D
and 2B4, which trigger NK cell responses (Bauer S. et al., supra; Nakajima H.
and Colonna M., 2000, 2B4: an NK cell activating receptor with unique specificity and signal transduction mechanism. Hum Immunol. 61:39-43; Bakker AB, Wu J, Phillips JH, and Lanier LL., 2000, NK cell activation: distinct stimulatory pathways counterbalancing inhibitory signals. Hum Inmzunol 61:18-27).
Remarkably, inhibition of TREM-1-mediated functions in LPS-induced shock is sufficient to reduce serum TNF-a and IL-113 to sublethal levels, preventing shock and death. In addition, mTREM-1-IgG I protects against bacterial peritonitis, in contrast to Prophylactic treatment with anti-TNF-a antibodies (Beutler, B., Milsark, I. W.
& Cerami, A. C., 1985, Passive immunization against cachectin/tumor necrosis factor protects mice from lethal effect of endotoxin. Science 229:869-71) or IL-1R antagonists, which increase lethality (Ohlsson, K., Bjork, P., Bergenfeldt, M., Hageman, R. & Thompson, R.
C., 1990, Interleukin-1 receptor antagonist reduces mortality from endotoxin shock.
Nature 348:550-2; Alexander, H. R., Doherty, G. M., Buresh, C. M., Venzon, D. J. & Norton, J.
A., 1991, A
recombinant human receptor antagonist to interleukin 1 improves survival after lethal endotoxemia in mice. J. Exp. Med. 173:1029-32; McNamara, M. J., Norton, Nauta, R.
J. & Alexander, H. R., 1993, Interleukin-1 receptor antibody (IL-lrab) protection and treatment against lethal endotoxemia in mice. J. Surg. Res. 54:316-21). Most likely, the residual levels of TNF-a and IL-113 after unTREM-1 treatment are sufficient for clearance of bacterial infections (Echtenather, B. a al., 1990, supra; Echtenacher, B. a at, 1996, supra; ivfalaviya, R. a. at, 1996, supra; Rothe, J. a at, 1993, supra;
Pfeffer, K. a at, 1993, supra; Peschon, J. J. et al., 1998, supra; Eskandari, M. K. etal., 1992, supra).
Importantly, rnTREM-1-IgG I was even protective after injection of LPS, an effect that was previously only reported for inhibition of MIF and HMG-I (Wang, H. a at, 1999, supra;
Calandra, T. et at, 2000, supra). Thus, post-infection administration of soluble TREM-1 might be a suitable therapeutic tool for the treatment of septic shock as well as other microbial-mediated diseases. The results of these studies demonstrate a central role of TREM-I in the amplification Of inflammatory responses to bacteria and fungi and provide a promising new strategy for the management of patients with acute inflammations and sepsis.
On the other hand, the studies have demonstrated that TREM-2 is significantly involved in DC functions including its maturation, migration to lymph nodes, presentation of antigens to T cells, and, thus, overall initiation of adaptive immune responses. Accordingly, interference with TREM-2's functions or its expression on DCs should be able to modulate the immune responses of the host, thus controlling various immune disorders, including autoirnmune diseases (e.g, systemic lupus arythernatosus, multiple sclerosis, dermatomiositis, rheumatoid arthritis, and allergies) and allergic disorders.
D. EOUIVALENTS
Those skilled in the art will recognize, or be able to ascertain many equivalents to the specific embodiments of the invention described herein using no more than routine experimentation. The scope of the claims should not be limited by the embodiments set forth in the examples, but should be given the broadest interpretation consistent with the description as a whole.

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Claims (6)

Claims:

1. A use of an antagonist of TREM-2 polypeptide for the treatment of inflammatory bowel disease, wherein the antagonist comprises a fusion protein of:
(a) the extracellular domain of TREM-2 polypeptide; and (b) a second polypeptide which is an immunoglobulin or a fragment thereof, wherein the fragment thereof comprises a heavy chain constant domain 2 or 3 (CH2 or CH3).

2. The use of an antagonist of TREM-2 polypeptide according to claim 1 further comprising the co-use of at least one other prophylactic or therapeutic agent.

3. The use of claim 1, wherein the immunoglobulin is hulgG or hulgM and the immunoglobulin fragment is a fragment of hulgG or a fragment of hulgM.

4. The use of claim 1, wherein the immunoglobulin fragment is the Fc region of the immunoglobulin.

5. The use of claim 1, wherein the extracellular domain of TREM-2 polypeptide consists of amino acids 19 to 171 of murine TREM-2.

6. The use of claim 1, wherein the extracellular domain of TREM-2 polypeptide consists of amino acids 14 to 167 of human TREM-2 (SEQ ID NO: 4).