CA2521497A1 - Modulation of cytokinin activity in plants - Google Patents
Modulation of cytokinin activity in plants Download PDFInfo
- Publication number
- CA2521497A1 CA2521497A1 CA002521497A CA2521497A CA2521497A1 CA 2521497 A1 CA2521497 A1 CA 2521497A1 CA 002521497 A CA002521497 A CA 002521497A CA 2521497 A CA2521497 A CA 2521497A CA 2521497 A1 CA2521497 A1 CA 2521497A1
- Authority
- CA
- Canada
- Prior art keywords
- cytokinin
- promoter
- plant
- transgenic plant
- expression cassette
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Granted
Links
- UQHKFADEQIVWID-UHFFFAOYSA-N cytokinin Natural products C1=NC=2C(NCC=C(CO)C)=NC=NC=2N1C1CC(O)C(CO)O1 UQHKFADEQIVWID-UHFFFAOYSA-N 0.000 title claims abstract 45
- 239000004062 cytokinin Substances 0.000 title claims abstract 45
- 230000000694 effects Effects 0.000 title claims 8
- 108090000623 proteins and genes Proteins 0.000 claims abstract 39
- 238000000034 method Methods 0.000 claims abstract 38
- 230000009261 transgenic effect Effects 0.000 claims abstract 31
- 230000014509 gene expression Effects 0.000 claims abstract 17
- 239000002773 nucleotide Substances 0.000 claims abstract 15
- 125000003729 nucleotide group Chemical group 0.000 claims abstract 15
- 230000003247 decreasing effect Effects 0.000 claims abstract 11
- 230000001850 reproductive effect Effects 0.000 claims abstract 9
- 230000001105 regulatory effect Effects 0.000 claims abstract 4
- 230000001172 regenerating effect Effects 0.000 claims abstract 3
- 230000001131 transforming effect Effects 0.000 claims abstract 2
- 241000196324 Embryophyta Species 0.000 claims 44
- 108091033319 polynucleotide Proteins 0.000 claims 31
- 239000002157 polynucleotide Substances 0.000 claims 31
- 102000040430 polynucleotide Human genes 0.000 claims 31
- 102000004169 proteins and genes Human genes 0.000 claims 24
- 238000003259 recombinant expression Methods 0.000 claims 24
- 230000015572 biosynthetic process Effects 0.000 claims 13
- 108090000790 Enzymes Proteins 0.000 claims 8
- 102000004190 Enzymes Human genes 0.000 claims 8
- 230000009466 transformation Effects 0.000 claims 7
- 239000003623 enhancer Substances 0.000 claims 6
- 230000002068 genetic effect Effects 0.000 claims 6
- 230000010152 pollination Effects 0.000 claims 5
- 241000701489 Cauliflower mosaic virus Species 0.000 claims 4
- 108700028146 Genetic Enhancer Elements Proteins 0.000 claims 4
- 230000001851 biosynthetic effect Effects 0.000 claims 4
- 230000001925 catabolic effect Effects 0.000 claims 4
- 239000012634 fragment Substances 0.000 claims 4
- 101100342815 Caenorhabditis elegans lec-1 gene Proteins 0.000 claims 3
- 101710118613 DNA polymerase sliding clamp 2 Proteins 0.000 claims 3
- 101000882584 Homo sapiens Estrogen receptor Proteins 0.000 claims 3
- 230000001627 detrimental effect Effects 0.000 claims 3
- 230000002123 temporal effect Effects 0.000 claims 3
- 238000013518 transcription Methods 0.000 claims 3
- 230000035897 transcription Effects 0.000 claims 3
- 241000589158 Agrobacterium Species 0.000 claims 2
- 108091026890 Coding region Proteins 0.000 claims 2
- 108091030071 RNAI Proteins 0.000 claims 2
- 240000008042 Zea mays Species 0.000 claims 2
- 230000036579 abiotic stress Effects 0.000 claims 2
- 108010050516 adenylate isopentenyltransferase Proteins 0.000 claims 2
- 239000000556 agonist Substances 0.000 claims 2
- 239000005557 antagonist Substances 0.000 claims 2
- 230000000692 anti-sense effect Effects 0.000 claims 2
- 108010088245 cytokinin oxidase Proteins 0.000 claims 2
- 230000030279 gene silencing Effects 0.000 claims 2
- 230000009368 gene silencing by RNA Effects 0.000 claims 2
- 230000001404 mediated effect Effects 0.000 claims 2
- 239000002245 particle Substances 0.000 claims 2
- 230000032361 posttranscriptional gene silencing Effects 0.000 claims 2
- 108020004511 Recombinant DNA Proteins 0.000 claims 1
- XUIMIQQOPSSXEZ-UHFFFAOYSA-N Silicon Chemical compound [Si] XUIMIQQOPSSXEZ-UHFFFAOYSA-N 0.000 claims 1
- 235000016383 Zea mays subsp huehuetenangensis Nutrition 0.000 claims 1
- 235000002017 Zea mays subsp mays Nutrition 0.000 claims 1
- 230000001488 breeding effect Effects 0.000 claims 1
- 238000004520 electroporation Methods 0.000 claims 1
- 239000000835 fiber Substances 0.000 claims 1
- 235000009973 maize Nutrition 0.000 claims 1
- 238000004519 manufacturing process Methods 0.000 claims 1
- 238000000520 microinjection Methods 0.000 claims 1
- 230000000306 recurrent effect Effects 0.000 claims 1
- 229910052710 silicon Inorganic materials 0.000 claims 1
- 239000010703 silicon Substances 0.000 claims 1
- 230000035882 stress Effects 0.000 claims 1
- 108091028043 Nucleic acid sequence Proteins 0.000 abstract 2
- 239000000203 mixture Substances 0.000 abstract 2
- 206010000210 abortion Diseases 0.000 abstract 1
- 231100000176 abortion Toxicity 0.000 abstract 1
- 239000003153 chemical reaction reagent Substances 0.000 abstract 1
- 230000007613 environmental effect Effects 0.000 abstract 1
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
- C12N9/0004—Oxidoreductases (1.)
- C12N9/0012—Oxidoreductases (1.) acting on nitrogen containing compounds as donors (1.4, 1.5, 1.6, 1.7)
- C12N9/0026—Oxidoreductases (1.) acting on nitrogen containing compounds as donors (1.4, 1.5, 1.6, 1.7) acting on CH-NH groups of donors (1.5)
- C12N9/0032—Oxidoreductases (1.) acting on nitrogen containing compounds as donors (1.4, 1.5, 1.6, 1.7) acting on CH-NH groups of donors (1.5) with oxygen as acceptor (1.5.3)
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
- C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
- C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
- C12N15/8216—Methods for controlling, regulating or enhancing expression of transgenes in plant cells
- C12N15/8222—Developmentally regulated expression systems, tissue, organ specific, temporal or spatial regulation
- C12N15/823—Reproductive tissue-specific promoters
- C12N15/8234—Seed-specific, e.g. embryo, endosperm
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
- C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
- C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
- C12N15/8241—Phenotypically and genetically modified plants via recombinant DNA technology
- C12N15/8261—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
- C12N9/10—Transferases (2.)
- C12N9/1085—Transferases (2.) transferring alkyl or aryl groups other than methyl groups (2.5)
-
- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02A—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE
- Y02A40/00—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production
- Y02A40/10—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production in agriculture
- Y02A40/146—Genetically Modified [GMO] plants, e.g. transgenic plants
Landscapes
- Health & Medical Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Genetics & Genomics (AREA)
- Engineering & Computer Science (AREA)
- Chemical & Material Sciences (AREA)
- Zoology (AREA)
- Organic Chemistry (AREA)
- Biomedical Technology (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Wood Science & Technology (AREA)
- Biotechnology (AREA)
- General Engineering & Computer Science (AREA)
- Molecular Biology (AREA)
- Microbiology (AREA)
- Biochemistry (AREA)
- General Health & Medical Sciences (AREA)
- Biophysics (AREA)
- Cell Biology (AREA)
- Physics & Mathematics (AREA)
- Medicinal Chemistry (AREA)
- Plant Pathology (AREA)
- Developmental Biology & Embryology (AREA)
- Pregnancy & Childbirth (AREA)
- Reproductive Health (AREA)
- Breeding Of Plants And Reproduction By Means Of Culturing (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
Abstract
This invention relates generally to the field of plant molecular biology. More specifically, this invention relates to methods and reagents for the temporally- and/or spatially-regulated expression of genes that affect metabolically effective levels of cytokinins in plants, particularly in plant seeds and related female reproductive tissue. This invention further relates to transgenic plants having enhanced levels of cytokinin expression wherein the transgenic plant exhibits useful characteristics, such as improved seed size, decreased tip kernel abortion, increased seed set during unfavorable environmental conditions, or stability of yield. The present invention also provides compositions and methods for regulating expression of heterologous nucleotide sequences in a plant. Compositions comprise novel nucleotide sequences for seed-preferred promoters known as eep1 and eep2. A method for expressing a heterologous nucleotide sequence in a plant using the promoter sequences disclosed herein is provided. The method comprises transforming a plant cell to comprise a heterologous nucleotide sequence operably linked to one of the promoters of the present invention and regenerating a stably transformed plant from the transformed plant cell.
Claims (65)
1. A method for producing transgenic plants capable of the regulated expression of a cytokinin-modulating gene in developing seed or related female reproductive tissue comprising:
transformation of plant host cells with a genetic construct capable of temporally- or spatially-regulated expression of a cytokinin modulating gene in developing seed or related female reproductive tissue; and regenerating and recovering said transgenic plants.
transformation of plant host cells with a genetic construct capable of temporally- or spatially-regulated expression of a cytokinin modulating gene in developing seed or related female reproductive tissue; and regenerating and recovering said transgenic plants.
2. The method according to Claim 1 wherein the transformation is carried out by a process selected from the group consisting of electroporation, PEG
poration, particle bombardment, silicon fiber delivery, microinjection, and Agrobacterium-mediated transformation.
poration, particle bombardment, silicon fiber delivery, microinjection, and Agrobacterium-mediated transformation.
3. The method according to Claim 2 wherein said process of transformation is particle bombardment.
4. The method according to Claim 2 wherein said process of transformation is Agrobacterium-mediated transformation.
5. The method according to Claim 1 wherein said genetic construct comprises a promoter directing temporal or spatial gene expression in developing seed or related female reproductive tissue, operably linked to a cytokinin-modulating gene.
6. The method according to Claim 5 wherein said promoter is selected from the group consisting of zag2.1, zap, tb1, eep1, eep2, F3.7, thxH, Zm40, ESR, PCNA2, lec1, ZmCkx1-2, ZmCkx2, ZmCkx3, ZmCkx4, and ZmCkx5.
7. The method according to Claim 1 wherein the cytokinin modulating gene is selected from the group consisting of genes encoding cytokinin biosynthetic enzymes, cytokinin catabolic enzymes, cytokinin catabolic enzyme antagonists and cytokinin biosynthetic enzyme agonists.
8. A transgenic plant comprising a genetic construct stably integrated into the genome thereof, said construct comprising a promoter operably linked to a cytokinin-modulating gene, wherein said promoter directs temporal or spatial expression in developing seed or related female reproductive tissues of said plant.
9. The plant according to Claim 8 wherein said promoter is selected from the group consisting of zag2.1, zap, tb1, eep1, eep2, F3.7, thxH, Zm40, ESR, PCNA2, lec1, ZmCkx1-2, ZmCkx2, ZmCkx3, ZmCkx4, and ZmCkx5.
10. The plant according to Claim 9 wherein the cytokinin modulating gene is selected from the group consisting of genes encoding cytokinin biosynthetic enzymes, cytokinin catabolic enzymes, cytokinin catabolic enzyme antagonists and cytokinin biosynthetic enzyme agonists.
11. An isolated recombinant DNA comprising a genetic construct comprising a promoter directing temporal or spatial gene expression in developing plant seed or related female reproductive tissue operably linked to a cytokinin modulating gene.
12. Host cells having stably introduced therein the genetic construct of Claim 11.
13. A method for improving stress tolerance and yield stability in plants comprising:
transformation of plant host cells with a genetic construct driving preferential expression of cytokinin-modulating genes in developing seed and related female reproductive tissue;
and regenerating and recovering tranformed plants from said cells.
transformation of plant host cells with a genetic construct driving preferential expression of cytokinin-modulating genes in developing seed and related female reproductive tissue;
and regenerating and recovering tranformed plants from said cells.
14. The method according to Claim 13 wherein said preferential expression occurs from about 14 days prior to about 25 days after pollination.
15. The method according to Claim 13 wherein said preferential expression occurs from about 0 to about 6 days after pollination.
16. The method according to Claim 13 wherein said preferential expression occurs from about 0 to about 12 days after pollination.
17. The method according to Claim 13 wherein said preferential expression occurs from about 4 to about 21 days after pollination.
18. A transgenic plant comprising a recombinant expression cassette stably integrated into the genome thereof, said cassette capable of effecting an increase in cytokinin activity, wherein said transgenic plant displays enhanced vigor without significant detrimental effects of increased cytokinin activity.
19. Seeds of the transgenic plant of Claim 18.
20. The transgenic plant of Claim 18, wherein said enhanced vigor is expressed in the presence or absence of abiotic stress.
21. A method of developing a maize plant utilizing the plant of Claim 18 as a source of genetic material in a breeding program.
22. The method of Claim 21 further comprising one or more techniques selected from the group consisting of: recurrent selection, mass selection, bulk selection, backcross, pedigree, development of a synthetic, and open pollination.
23. The transgenic plant of Claim 18, wherein said recombinant expression cassette comprises a polynucleotide encoding a protein involved in cytokinin biosynthesis.
24. The transgenic plant of Claim 18, wherein said recombinant expression cassette comprises a polynucleotide encoding isopentenyl transferase.
25. The transgenic plant of Claim 23, wherein said recombinant expression cassette comprises the zag2.1 promoter operably linked to a polynucleotide encoding a protein involved in cytokinin biosynthesis.
26. The transgenic plant of Claim 23, wherein said recombinant expression cassette comprises the eep1 promoter operably linked to a polynucleotide encoding a protein involved in cytokinin biosynthesis.
27. The transgenic plant of Claim 23, wherein said recombinant expression cassette comprises the eep2 promoter operably linked to a polynucleotide encoding a protein involved in cytokinin biosynthesis.
28. The transgenic plant of Claim 23, wherein said recombinant expression cassette comprises the zap promoter operably linked to a polynucleotide encoding a protein involved in cytokinin biosynthesis.
29. The transgenic plant of Claim 23, wherein said recombinant expression cassette comprises the tb1 promoter operably linked to a polynucleotide encoding a protein involved in cytokinin biosynthesis.
30. The transgenic plant of Claim 23, wherein said recombinant expression cassette comprises the ckx1-2 promoter operably linked to a polynucleotide encoding a protein involved in cytokinin biosynthesis.
31. The transgenic plant of Claim 23, wherein said recombinant expression cassette comprises a promoter which drives low-level constitutive expression of an operably-linked polynucleotide encoding a protein involved in cytokinin biosynthesis.
32. The transgenic plant of Claim 23, wherein said recombinant expression cassette comprises the F3.7 promoter.
33. The transgenic plant of Claim 23, wherein said recombinant expression cassette comprises (1) a reproductive-tissue-preferred promoter operably linked to a polynucleotide encoding a protein involved in cytokinin biosynthesis and (2) one or more promoters or enhancer elements of a highly-expressed gene.
34. The transgenic plant of Claim 33 wherein the enhancer element comprises the 35S enhancer of cauliflower mosaic virus.
35. The transgenic plant of Claim 34 wherein the 35S enhancer comprises SEQ
ID NO: 4.
ID NO: 4.
36. The transgenic plant of Claim 33 wherein the recombinant expression cassette comprises (1) the zag2.1 promoter operably linked to a polynucleotide encoding ipt and (2) the cauliflower mosaic virus 35S
enhancer.
enhancer.
37. The transgenic plant of Claim 36 wherein the recombinant expression cassette comprises (1) SEQ ID NO: 3 operably linked to the coding region of SEQ ID NO: 1 and (2) SEQ ID NO: 4.
38. The transgenic plant of Claim 18, wherein said recombinant expression cassette comprises a first polynucleotide involved in silencing of a gene encoding a protein involved in decreasing the active cytokinin pool.
39. The transgenic plant of Claim 38, wherein said protein involved in decreasing the active cytokinin pool is cytokinin oxidase.
40. The transgenic plant of Claim 38, further comprising a promoter preferentially driving expression in developing seed or related female reproductive tissues, operably linked to a second polynucleotide encoding a protein involved in cytokinin biosynthesis.
41. The transgenic plant of Claim 38, wherein said first polynucleotide comprises an antisense sequence for a polynucleotide encoding a protein involved in decreasing the active cytokinin pool.
42. The transgenic plant of Claim 38, wherein said first polynucleotide comprises a sequence effective in cosuppression of a polynucleotide encoding a protein involved in decreasing the active cytokinin pool.
43. The transgenic plant of Claim 38, wherein said first polynucleotide comprises a sequence effective in RNAi of a polynucleotide encoding a protein involved in decreasing the active cytokinin pool.
44. A method of modulating cytokinin activity in a plant, wherein modulated cytokinin activity enhances plant vigor without significant detrimental effects, comprising stably transforming said plant to result in an increase in cytokinin activity.
45. The method of Claim 44 wherein said plant is stably transformed with a recombinant expression cassette capable of effecting an increase in cytokinin activity, wherein said transgenic plant displays enhanced vigor without significant detrimental effects of increased cytokinin activity.
46. The method of Claim 45, wherein said enhanced vigor is expressed in the presence or absence of abiotic stress.
47. The method of Claim 45, wherein said recombinant expression cassette comprises a polynucleotide encoding a protein involved in cytokinin biosynthesis.
48. The method of Claim 45, wherein said recombinant expression cassette comprises a polynucleotide encoding isopentenyl transferase.
49. The method of Claim 45, wherein said recombinant expression cassette comprises a promoter selected from the group consisting of zag2.1, zap, tb1, eep1, eep2, F3.7, thxH, Zm40, ESR, PCNA2, lec1, ZmCkx1-2, ZmCkx2, ZmCkx3, ZmCkx4, and ZmCkx5.
50. The method of Claim 45, wherein said recombinant expression cassette comprises (1) a female reproductive-tissue-preferred promoter operably linked to a polynucleotide encoding a protein involved in cytokinin biosynthesis and (2) one or more promoters or enhancer elements of a highly-expressed gene.
51. The method of Claim 50 wherein the enhancer element comprises the 35S
enhancer of cauliflower mosaic virus.
enhancer of cauliflower mosaic virus.
52. The method of Claim 51 wherein the 35S enhancer comprises SEQ ID NO: 4.
53. The method of Claim 50 wherein the recombinant expression cassette comprises (1) the zag2.1 promoter operably linked to a polynucleotide encoding ipt and (2) the cauliflower mosaic virus 35S enhancer.
54. The method of Claim 53 wherein the recombinant expression cassette comprises (1) SEQ ID NO: 3 operably linked to the coding region of SEQ ID
NO: 1 and (2) SEQ ID NO: 4.
NO: 1 and (2) SEQ ID NO: 4.
55. The method of Claim 45, wherein said recombinant expression cassette comprises a first polynucleotide involved in silencing of a gene encoding a protein involved in decreasing the active cytokinin pool.
56. The method of Claim 55, wherein said protein involved in decreasing the active cytokinin pool is cytokinin oxidase.
57. The method of Claim 55, wherein said plant is further stably transformed with a promoter preferentially driving expression in developing seed or related female reproductive tissues, operably linked to a second polynucleotide encoding a protein involved in cytokinin biosynthesis.
58. The method of Claim 55, wherein said first polynucleotide comprises an antisense sequence for a polynucleotide encoding a protein involved in decreasing the active cytokinin pool.
59. The method of Claim 55, wherein said first polynucleotide comprises a sequence effective in cosuppression of a polynucleotide encoding a protein involved in decreasing the active cytokinin pool.
60. The method of Claim 55, wherein said first polynucleotide comprises a sequence effective in RNAi of a polynucleotide encoding a protein involved in decreasing the active cytokinin pool.
61. An isolated promoter capable of driving transcription in a seed-preferred manner, wherein the promoter comprises a nucleotide sequence selected from the group consisting of:
a sequence comprising a fragment of the nucleotide sequence set forth in SEQ ID NO: 7 or 18; and the nucleotide sequence set forth in SEQ ID NO: 7 or 18.
a sequence comprising a fragment of the nucleotide sequence set forth in SEQ ID NO: 7 or 18; and the nucleotide sequence set forth in SEQ ID NO: 7 or 18.
62. The isolated promoter of Claim 61 capable of driving transcription in a seed-preferred manner, wherein said promoter comprises a fragment of the nucleotide sequence set forth in SEQ ID NO: 7 or 18.
63. The isolated promoter of Claim 61 capable of driving transcription in a seed-preferred manner, wherein said promoter comprises the nucleotide sequence set forth in SEQ ID NO: 7 or 18.
64. A recombinant expression cassette comprising a promoter and a nucleotide sequence operably linked to the promoter, wherein the promoter comprises a nucleotide sequence selected from the group consisting of:
a sequence comprising a fragment of the nucleotide sequence set forth in SEQ ID NO: 7 or 18; and the nucleotide sequence set forth in SEQ ID NO: 7 or 18.
a sequence comprising a fragment of the nucleotide sequence set forth in SEQ ID NO: 7 or 18; and the nucleotide sequence set forth in SEQ ID NO: 7 or 18.
65. A plant stably transformed with an expression cassette comprising a maize promoter and a nucleotide sequence operably linked to the promoter, wherein said promoter comprises a nucleotide sequence selected from the group consisting of:
a sequence comprising a fragment of the nucleotide sequence set forth in SEQ ID NO: 7 or 18; and the nucleotide sequence set forth in SEQ ID NO: 7 or 18.
a sequence comprising a fragment of the nucleotide sequence set forth in SEQ ID NO: 7 or 18; and the nucleotide sequence set forth in SEQ ID NO: 7 or 18.
Applications Claiming Priority (3)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
US46071803P | 2003-04-04 | 2003-04-04 | |
US60/460,718 | 2003-04-04 | ||
PCT/US2004/010064 WO2004090143A2 (en) | 2003-04-04 | 2004-04-02 | Modulation of cytokinin activity in plants |
Publications (2)
Publication Number | Publication Date |
---|---|
CA2521497A1 true CA2521497A1 (en) | 2004-10-21 |
CA2521497C CA2521497C (en) | 2012-11-27 |
Family
ID=33159802
Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
CA2521497A Expired - Fee Related CA2521497C (en) | 2003-04-04 | 2004-04-02 | Modulation of cytokinin activity in plants |
Country Status (9)
Country | Link |
---|---|
EP (1) | EP1613754A2 (en) |
CN (3) | CN101220358A (en) |
AR (1) | AR045420A1 (en) |
AU (1) | AU2004227360B2 (en) |
BR (1) | BRPI0409178A (en) |
CA (1) | CA2521497C (en) |
NZ (1) | NZ543070A (en) |
WO (1) | WO2004090143A2 (en) |
ZA (1) | ZA200508006B (en) |
Families Citing this family (53)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
US7371925B2 (en) * | 2001-03-29 | 2008-05-13 | Pioneer Hi-Bred International, Inc. | Cytokinin oxidase promoter from maize |
EP1520028A4 (en) * | 2002-06-19 | 2006-08-16 | Basf Plant Science Gmbh | Compositions and methods for improving plant performance |
EP1741788A1 (en) * | 2005-07-06 | 2007-01-10 | Freie Universität Berlin | Transgenic plant with tissue-selectively reduced cytokinin receptor activity |
US20080301839A1 (en) * | 2005-08-30 | 2008-12-04 | Ravanello Monica P | Transgenic plants with enhanced agronomic traits |
CA2743707A1 (en) | 2008-12-04 | 2010-06-10 | Pioneer Hi-Bred International, Inc. | Methods and compositions for enhanced yield by targeted expression of knotted1 |
WO2010147825A1 (en) | 2009-06-09 | 2010-12-23 | Pioneer Hi-Bred International, Inc. | Early endosperm promoter and methods of use |
US8440891B2 (en) | 2009-09-22 | 2013-05-14 | Board of Trustees of the University of Akransas, N.A. | Rice cultivar CL 142-AR |
US8440892B2 (en) | 2009-10-15 | 2013-05-14 | Board Of Trustees Of The University Of Arkansas, N.A. | Rice cultivar CL 181-AR |
WO2011056544A1 (en) | 2009-10-26 | 2011-05-12 | Pioneer Hi-Bred International, Inc. | Somatic ovule specific promoter and methods of use |
WO2011084621A1 (en) * | 2009-12-17 | 2011-07-14 | Pioneer Hi-Bred International, Inc. | Maize event dp-004114-3 and methods for detection thereof |
CN101899442B (en) * | 2010-07-13 | 2012-02-01 | 中国科学院遗传与发育生物学研究所 | Rice RR17 promoter and application thereof |
US20130167262A1 (en) | 2011-12-21 | 2013-06-27 | The Curators Of The University Of Missouri | Soybean variety s05-11268 |
US9204603B2 (en) | 2011-12-21 | 2015-12-08 | The Curators Of The University Of Missouri | Soybean variety S05-11482 |
US9006515B2 (en) | 2012-01-06 | 2015-04-14 | Pioneer Hi Bred International Inc | Pollen preferred promoters and methods of use |
WO2013103371A1 (en) | 2012-01-06 | 2013-07-11 | Pioneer Hi-Bred International, Inc. | Ovule specific promoter and methods of use |
MX2014011044A (en) * | 2012-03-13 | 2015-05-15 | Pioneer Hi Bred Int | Genetic reduction of male fertility in plants. |
CN102629304B (en) * | 2012-04-05 | 2015-02-04 | 天津大学 | Genome scale metabolic network model-based metabolic engineering design prediction method |
US20140109259A1 (en) | 2012-10-11 | 2014-04-17 | Pioneer Hi Bred International Inc | Guard Cell Promoters and Uses Thereof |
EA028528B1 (en) | 2013-03-13 | 2017-11-30 | Пайонир Хай-Бред Интернэшнл, Инк. | Glyphosate application for weed control in brassica |
CN115960896A (en) | 2013-03-14 | 2023-04-14 | 先锋国际良种公司 | Compositions and methods for controlling insect pests |
WO2014150914A2 (en) | 2013-03-15 | 2014-09-25 | Pioneer Hi-Bred International, Inc. | Phi-4 polypeptides and methods for their use |
EA030896B1 (en) | 2013-08-16 | 2018-10-31 | Пайонир Хай-Бред Интернэшнл, Инк. | Insecticidal proteins and methods for their use |
BR122020001770B1 (en) | 2013-09-13 | 2022-11-29 | Pioneer Hi-Bred International, Inc | DNA CONSTRUCTION, METHOD FOR OBTAINING A TRANSGENIC PLANT, FUSION PROTEIN, METHOD FOR CONTROLLING AN INSECT PEST POPULATION, METHOD FOR INHIBITING THE GROWTH OR KILLING AN INSECT PEST |
US10227608B2 (en) | 2014-02-07 | 2019-03-12 | Pioneer Hi-Bred International, Inc. | Insecticidal proteins from plants and methods for their use |
US20170218384A1 (en) | 2014-08-08 | 2017-08-03 | Pioneer Hi-Bred International, Inc. | Ubiquitin promoters and introns and methods of use |
CA2961733A1 (en) | 2014-09-17 | 2016-03-24 | Pioneer Hi-Bred International, Inc. | Compositions and methods to control insect pests |
US10435706B2 (en) | 2014-10-16 | 2019-10-08 | Pioneer Hi-Bred International, Inc. | Insecticidal proteins and methods for their use |
WO2016099916A1 (en) | 2014-12-19 | 2016-06-23 | E. I. Du Pont De Nemours And Company | Polylactic acid compositions with accelerated degradation rate and increased heat stability |
CN108064233B (en) | 2015-05-19 | 2022-07-15 | 先锋国际良种公司 | Insecticidal proteins and methods of use thereof |
CA2986265A1 (en) | 2015-06-16 | 2016-12-22 | Pioneer Hi-Bred International, Inc. | Compositions and methods to control insect pests |
CA2994676A1 (en) | 2015-08-06 | 2017-02-09 | Pioneer Hi-Bred International, Inc. | Plant derived insecticidal proteins and methods for their use |
ES2773072T3 (en) | 2015-08-28 | 2020-07-09 | Pioneer Hi Bred Int | Plant transformation mediated by Ochrobactrum |
CN108575091A (en) | 2015-12-18 | 2018-09-25 | 先锋国际良种公司 | Insecticidal protein and its application method |
US11104911B2 (en) | 2015-12-22 | 2021-08-31 | Pioneer Hi-Bred International, Inc. | Embryo-preferred Zea mays promoters and methods of use |
CN105624190A (en) * | 2016-03-23 | 2016-06-01 | 东北农业大学 | Breeding method for isopentenyl-acyltransferase transgenic corn |
EA201892293A1 (en) | 2016-05-04 | 2019-04-30 | Пайонир Хай-Бред Интернэшнл, Инк. | INSECTICIDAL PROTEINS AND METHODS OF THEIR APPLICATION |
CN109312359A (en) | 2016-06-16 | 2019-02-05 | 先锋国际良种公司 | To prevent and treat the composition and method of insect pest |
MX2018015900A (en) | 2016-06-24 | 2019-04-04 | Pioneer Hi Bred Int | Plant regulatory elements and methods of use thereof. |
EP3954202A1 (en) | 2016-07-01 | 2022-02-16 | Pioneer Hi-Bred International, Inc. | Insecticidal proteins from plants and methods for their use |
US20210292778A1 (en) | 2016-07-12 | 2021-09-23 | Pioneer Hi-Bred International, Inc. | Compositions and methods to control insect pests |
CN109863167B (en) | 2016-11-01 | 2022-12-23 | 先锋国际良种公司 | Insecticidal proteins and methods of use thereof |
CA3076831A1 (en) | 2017-09-25 | 2019-03-28 | Pioneer Hi-Bred International, Inc. | Tissue-preferred promoters and methods of use |
WO2019178042A1 (en) | 2018-03-14 | 2019-09-19 | Pioneer Hi-Bred International, Inc. | Insecticidal proteins from plants and methods for their use |
CA3092075A1 (en) | 2018-03-14 | 2019-09-19 | Pioneer Hi-Bred International, Inc. | Insecticidal proteins from plants and methods for their use |
US11702668B2 (en) | 2018-05-22 | 2023-07-18 | Pioneer Hi-Bred International, Inc. | Plant regulatory elements and methods of use thereof |
EP3833747A1 (en) | 2018-06-28 | 2021-06-16 | Pioneer Hi-Bred International, Inc. | Methods for selecting transformed plants |
AU2019369415A1 (en) | 2018-10-31 | 2021-03-25 | Pioneer Hi-Bred International, Inc. | Compositions and methods for Ochrobactrum-mediated plant transformation |
CN109762837B (en) * | 2018-12-24 | 2020-12-15 | 中国医学科学院药用植物研究所 | Method for regulating coenzyme Q synthesis by using salvia miltiorrhiza long-chain isopentenyl transferase gene SmPPS2 |
CN109735538B (en) * | 2018-12-27 | 2022-06-21 | 南京农业大学 | Carrier for improving forest strawberry leaf regeneration efficiency and preparation method and application thereof |
CN111518816B (en) * | 2020-05-12 | 2021-10-08 | 广西大学 | Corn dark response albino/glassy yellow 12 gene and application thereof |
US20230235352A1 (en) | 2020-07-14 | 2023-07-27 | Pioneer Hi-Bred International, Inc. | Insecticidal proteins and methods for their use |
CN114990112B (en) * | 2021-02-26 | 2024-03-12 | 华中农业大学 | Specific promoter for spiny skin |
CN114807181A (en) * | 2022-04-30 | 2022-07-29 | 浙江师范大学 | Application of rice OsCKX3 gene in regulation and control of rice leaf included angle |
Family Cites Families (21)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
US4945050A (en) | 1984-11-13 | 1990-07-31 | Cornell Research Foundation, Inc. | Method for transporting substances into living cells and tissues and apparatus therefor |
ATE57390T1 (en) | 1986-03-11 | 1990-10-15 | Plant Genetic Systems Nv | PLANT CELLS OBTAINED BY GENOLOGICAL TECHNOLOGY AND RESISTANT TO GLUTAMINE SYNTHETASE INHIBITORS. |
US5453566A (en) | 1986-03-28 | 1995-09-26 | Calgene, Inc. | Antisense regulation of gene expression in plant/cells |
US6329570B1 (en) * | 1989-07-19 | 2001-12-11 | Calgene, Llc | Cotton modification using ovary-tissue transcriptional factors |
ATE212667T1 (en) | 1990-04-26 | 2002-02-15 | Aventis Cropscience Nv | NEW BACILLUSTHURINGSIENSIS STRAIN AND ITS INSECT TOXIN-ENCODING GENE |
US6329520B1 (en) * | 1990-08-09 | 2001-12-11 | Research Triangle Institute | Cocaine receptor binding ligands |
US5277905A (en) | 1991-01-16 | 1994-01-11 | Mycogen Corporation | Coleopteran-active bacillus thuringiensis isolate |
KR100241117B1 (en) | 1991-08-02 | 2000-02-01 | 코헤이 미쯔이 | Novel microorganism and insecticide |
GB9210273D0 (en) | 1992-05-13 | 1992-07-01 | Ici Plc | Dna |
CA2127807A1 (en) | 1992-11-20 | 1994-06-09 | John Maliyakal | Transgenic cotton plants producing heterologous bioplastic |
US5593881A (en) | 1994-05-06 | 1997-01-14 | Mycogen Corporation | Bacillus thuringiensis delta-endotoxin |
US5736369A (en) | 1994-07-29 | 1998-04-07 | Pioneer Hi-Bred International, Inc. | Method for producing transgenic cereal plants |
US5792931A (en) | 1994-08-12 | 1998-08-11 | Pioneer Hi-Bred International, Inc. | Fumonisin detoxification compositions and methods |
US5689042A (en) * | 1995-03-29 | 1997-11-18 | Wisconsin Alumni Research Foundation | Transgenic plants with altered senescence characteristics |
NZ312976A (en) * | 1995-07-26 | 1999-06-29 | Pioneer Hi Bred Int | An expression control sequence for general and effective expression of genes in plants |
US5737514A (en) | 1995-11-29 | 1998-04-07 | Texas Micro, Inc. | Remote checkpoint memory system and protocol for fault-tolerant computer system |
EP1104469B1 (en) * | 1998-08-20 | 2005-11-09 | Pioneer Hi-Bred International, Inc. | Seed-preferred promoters |
US7531723B2 (en) | 1999-04-16 | 2009-05-12 | Pioneer Hi-Bred International, Inc. | Modulation of cytokinin activity in plants |
NZ514955A (en) * | 1999-04-16 | 2004-01-30 | Pioneer Hi Bred Int | Regulated expression of cytokinin modulating genes in plant seeds |
US6407315B1 (en) * | 1999-11-02 | 2002-06-18 | Pioneer Hi-Bred International, Inc. | Seed-preferred promoter from barley |
AU2002254415A1 (en) * | 2001-03-29 | 2002-10-15 | Pioneer Hi-Bred International, Inc. | Tissue-preferred promoter from maize |
-
2004
- 2004-04-02 CN CNA2008100089946A patent/CN101220358A/en active Pending
- 2004-04-02 CA CA2521497A patent/CA2521497C/en not_active Expired - Fee Related
- 2004-04-02 NZ NZ543070A patent/NZ543070A/en not_active IP Right Cessation
- 2004-04-02 CN CNA2008100089927A patent/CN101220357A/en active Pending
- 2004-04-02 BR BRPI0409178-7A patent/BRPI0409178A/en not_active Application Discontinuation
- 2004-04-02 CN CNA2004800155350A patent/CN1798843A/en active Pending
- 2004-04-02 WO PCT/US2004/010064 patent/WO2004090143A2/en active Application Filing
- 2004-04-02 EP EP04749636A patent/EP1613754A2/en not_active Withdrawn
- 2004-04-02 AU AU2004227360A patent/AU2004227360B2/en not_active Ceased
- 2004-04-06 AR ARP040101154A patent/AR045420A1/en active IP Right Grant
-
2005
- 2005-10-04 ZA ZA200508006A patent/ZA200508006B/en unknown
Also Published As
Publication number | Publication date |
---|---|
BRPI0409178A (en) | 2006-05-02 |
CN101220358A (en) | 2008-07-16 |
NZ543070A (en) | 2008-10-31 |
AU2004227360B2 (en) | 2009-05-28 |
CA2521497C (en) | 2012-11-27 |
AR045420A1 (en) | 2005-10-26 |
CN101220357A (en) | 2008-07-16 |
WO2004090143A2 (en) | 2004-10-21 |
AU2004227360A1 (en) | 2004-10-21 |
EP1613754A2 (en) | 2006-01-11 |
ZA200508006B (en) | 2007-01-31 |
CN1798843A (en) | 2006-07-05 |
WO2004090143A3 (en) | 2005-05-12 |
Similar Documents
Publication | Publication Date | Title |
---|---|---|
CA2521497A1 (en) | Modulation of cytokinin activity in plants | |
CA2366104C (en) | Ultra-fast transformation technique for monocotyledons | |
US8779238B2 (en) | Floral dip method for transformation of camelina | |
US6320102B1 (en) | Leafy cotyledon1 genes and their uses | |
AU2249901A (en) | Methods for conditional transgene expression and trait removal in plants | |
US7790950B2 (en) | Use of phytoene synthase for controlling transgene escape | |
AU2011273569B2 (en) | Gene involved in the development of the seed | |
US7267979B2 (en) | Method of controlling gene silencing using site specific recombination | |
CN105713079B (en) | Protein and its relevant biological material are improving the application in plant products | |
CN105985417B (en) | Application of the growth associated protein GRP2 in regulating plant growth | |
CN105504031B (en) | From the grain weight GAP-associated protein GAP and its relevant biological material of soybean and application | |
US7268271B2 (en) | Methods of use of LEC1 polynucleotides and polypeptides | |
CN105820220A (en) | Stress resistance relevant protein and application of coding gene in regulating alkali resistance of plants | |
CN106279386A (en) | A kind of Rice Panicle grown on top development associated protein and encoding gene thereof and application | |
CN105985418B (en) | Application of the growth associated protein GRP4 in regulating plant growth | |
CN105985419B (en) | Application of the growth associated protein GRP1 in regulating plant growth | |
CN108424911B (en) | Seed-specific bidirectional promoter and application thereof | |
US20030018995A1 (en) | Plants with a modified flower and seed development | |
EP1644507B1 (en) | Novel rubisco promoters and uses thereof | |
AU2018239406A1 (en) | Anther-specific promoter and uses thereof | |
US6822139B1 (en) | Modulation of storage organs | |
US20050081266A1 (en) | Modulation of storage organs | |
US20040049803A1 (en) | Senescence-associated plant promotors | |
CN105985420B (en) | Application of the growth associated protein GRP3 in regulating plant growth | |
KR101171044B1 (en) | Method for transformation using toxoflavin lyase genetflA as a selection marker and transgenic watermelon gongdae produced by the method |
Legal Events
Date | Code | Title | Description |
---|---|---|---|
EEER | Examination request | ||
MKLA | Lapsed |
Effective date: 20160404 |