CA2512279A1 - Method for preparing lactate biosensing strip - Google Patents

Method for preparing lactate biosensing strip Download PDF

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Publication number
CA2512279A1
CA2512279A1 CA002512279A CA2512279A CA2512279A1 CA 2512279 A1 CA2512279 A1 CA 2512279A1 CA 002512279 A CA002512279 A CA 002512279A CA 2512279 A CA2512279 A CA 2512279A CA 2512279 A1 CA2512279 A1 CA 2512279A1
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CA
Canada
Prior art keywords
silver
layer
electrode
graphite
layers
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Granted
Application number
CA002512279A
Other languages
French (fr)
Other versions
CA2512279C (en
Inventor
Manoj Kumar Pandey
Asha Chaubey
Krishan Kant Pande
Rajendra Kumar Sharma
Krishan Kumar Saini
Bansi Dhar Malhotra
Rajesh
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Council of Scientific and Industrial Research CSIR
Original Assignee
Council Of Scientific And Industrial Research
Manoj Kumar Pandey
Asha Chaubey
Krishan Kant Pande
Rajendra Kumar Sharma
Krishan Kumar Saini
Bansi Dhar Malhotra
Rajesh
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Council Of Scientific And Industrial Research, Manoj Kumar Pandey, Asha Chaubey, Krishan Kant Pande, Rajendra Kumar Sharma, Krishan Kumar Saini, Bansi Dhar Malhotra, Rajesh filed Critical Council Of Scientific And Industrial Research
Publication of CA2512279A1 publication Critical patent/CA2512279A1/en
Application granted granted Critical
Publication of CA2512279C publication Critical patent/CA2512279C/en
Anticipated expiration legal-status Critical
Expired - Fee Related legal-status Critical Current

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Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/001—Enzyme electrodes
    • C12Q1/002—Electrode membranes
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/001—Enzyme electrodes
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/001—Enzyme electrodes
    • C12Q1/005—Enzyme electrodes involving specific analytes or enzymes

Landscapes

  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Microbiology (AREA)
  • Biochemistry (AREA)
  • Physics & Mathematics (AREA)
  • Molecular Biology (AREA)
  • Biotechnology (AREA)
  • Biophysics (AREA)
  • Analytical Chemistry (AREA)
  • Immunology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Investigating Or Analysing Biological Materials (AREA)

Abstract

The present invention provides a method for the manufacture of a lactate biosensing strip for use in the assay of lactate in a sample, said sensor comprising a dry strip sensor of an electrically conducting material having at least: i) an external surface. ii) a screen printed reference electrode and, iii) a screen-printed working electrode.

Claims (9)

1. A method for the preparation of lactate biosensing strip comprising an electrically insulated base support (1), a pair of isolated first and second silver layers deposited thereon (2), a pair of graphite layers, each one of said pair of graphite layers being deposited on one respective silver layer and electrically connected to said respective silver layer (2), the first silver layer being covered fully by the respective graphite layer, the second silver layer being covered partly in the middle thereof with the respective graphite layer after leaving the terminal and working zone area uncovered, a Ag/AgCl electrode (4) provided on top of the working zone area of said second silver electrode layer, lactate oxidase deposited with a mediator on the working zone area of graphite layer covering the first silver layer (5), the said silver/silver chloride electrode (4) and enzyme with mediator layer working electrode (5) being supported on said support (1), the working zone of silver/silver chloride reference electrode (4) and working electrode (5) being covered with a hydrophilic membrane, said lactate biosensing strip of the invention showing an activity of 75% and a response time for lactate detection in the range of 30 to 40 seconds; shelf life of about 4 months under refrigerated conditions and about 2 months under ambient conditions (25 to 30°C), the strip of the invention being disposable, said process comprising (a) depositing a pair of silver layers on an electrically insulated base support;
(b) depositing a pair of graphite layers on said silver layers, each of said silver layers being deposited with one graphite layer, a first graphite layer completely covering the first silver layer, and the second graphite layer covering the second silver layer only in part of the surface thereof that is away from the surface facing the base support;
(c) depositing a silver chloride layer on the second silver layer on the part thereof that is not deposited with a graphite layer to obtain a silver/silver chloride electrode;
(d) adsorbing physically lactate oxidase enzyme with an electron mediator on the first graphite deposited silver layer to obtain a working electrode; and (e) applying an outer hydrophilic membrane on the above said first reference electrode and second working electrode to obtain the desired pair of electrodes on an electrically insulated base support in a single assembly.
2. A method as claimed in claim 1, wherein the electrically insulated base support used is polyvinyl chloride.
3. A method as claimed in claim 1, wherein the said silver layer is applied by the step of screen-printing.
4. A method as claimed in claim 1, wherein the said graphite layer is applied by the step of screen-printing.
5. A method as claimed in claim 1, wherein the sample being tested is an aqueous lactate solution or blood sample in an amount of 25 to 30µL.
6. A method as claimed in claim 1, wherein the electron mediator used is selected from potassium ferricyanide and ferrocene.
7. A method as claimed in claim 1, wherein the working zone are of electrode is a target area used for dispensing the analyte sample.
8. A method as claimed in claim 1, wherein the connecting terminal zone area of electrode is an area used for the connectivity of electrode to an electrometer.
9. A method as claimed in claim 1, wherein the hydrophilic membrane is made of nylon or polyester.
CA2512279A 2002-12-31 2002-12-31 Method for preparing lactate biosensing strip Expired - Fee Related CA2512279C (en)

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
PCT/IB2002/005677 WO2004058992A1 (en) 2002-12-31 2002-12-31 Method for preparing lactate biosensing strip

Publications (2)

Publication Number Publication Date
CA2512279A1 true CA2512279A1 (en) 2004-07-15
CA2512279C CA2512279C (en) 2010-06-29

Family

ID=32676718

Family Applications (1)

Application Number Title Priority Date Filing Date
CA2512279A Expired - Fee Related CA2512279C (en) 2002-12-31 2002-12-31 Method for preparing lactate biosensing strip

Country Status (6)

Country Link
EP (1) EP1578984A1 (en)
JP (1) JP2006512571A (en)
CN (1) CN100372940C (en)
AU (1) AU2002368512A1 (en)
CA (1) CA2512279C (en)
WO (1) WO2004058992A1 (en)

Families Citing this family (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
GB0606998D0 (en) * 2006-04-06 2006-05-17 Oxford Biosensors Ltd Lipoprotein sensor
CN101344501B (en) * 2008-08-22 2011-10-05 中南大学 Silk screen printing electrode, manufacturing technique and use thereof
JP2010230369A (en) * 2009-03-26 2010-10-14 Ryukoku Univ Electrode structure, manufacturing method of the same, and electrochemical sensor
CN101650331B (en) * 2009-08-27 2012-09-05 无锡爱康生物科技有限公司 Enzyme bioelectrochemical sensing chip and preparation method thereof
KR101947639B1 (en) * 2017-08-08 2019-02-14 한국화학연구원 Glucose sensor for ex-vivo diagnosis comprising graphene-based electrode
US20190320947A1 (en) * 2018-04-19 2019-10-24 Abbott Diabetes Care Inc. Lactate sensors and associated methods

Family Cites Families (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5682884A (en) * 1983-05-05 1997-11-04 Medisense, Inc. Strip electrode with screen printing
US5707502A (en) * 1996-07-12 1998-01-13 Chiron Diagnostics Corporation Sensors for measuring analyte concentrations and methods of making same
CO5040209A1 (en) * 1997-10-16 2001-05-29 Abbott Lab BIOSENSOR ELECTRODES MEDIATORS OF COFACTOR REGENERATION

Also Published As

Publication number Publication date
CN100372940C (en) 2008-03-05
CA2512279C (en) 2010-06-29
AU2002368512A1 (en) 2004-07-22
JP2006512571A (en) 2006-04-13
WO2004058992A1 (en) 2004-07-15
EP1578984A1 (en) 2005-09-28
CN1742092A (en) 2006-03-01

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Effective date: 20170103