CA2509769A1 - Procede et dispositif d'echantillonnage permettant d'analyser des reactions d'amplification - Google Patents

Procede et dispositif d'echantillonnage permettant d'analyser des reactions d'amplification Download PDF

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Publication number
CA2509769A1
CA2509769A1 CA002509769A CA2509769A CA2509769A1 CA 2509769 A1 CA2509769 A1 CA 2509769A1 CA 002509769 A CA002509769 A CA 002509769A CA 2509769 A CA2509769 A CA 2509769A CA 2509769 A1 CA2509769 A1 CA 2509769A1
Authority
CA
Canada
Prior art keywords
amplification
nucleic acid
aliquot
plural
sample
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Abandoned
Application number
CA002509769A
Other languages
English (en)
Inventor
Vladimir I. Slepnev
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
PRIMERA BIOSYSTEMS
Original Assignee
Individual
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Individual filed Critical Individual
Publication of CA2509769A1 publication Critical patent/CA2509769A1/fr
Abandoned legal-status Critical Current

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Classifications

    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L7/00—Heating or cooling apparatus; Heat insulating devices
    • B01L7/52—Heating or cooling apparatus; Heat insulating devices with provision for submitting samples to a predetermined sequence of different temperatures, e.g. for treating nucleic acid samples
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6844—Nucleic acid amplification reactions
    • C12Q1/6851—Quantitative amplification
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L3/00—Containers or dishes for laboratory use, e.g. laboratory glassware; Droppers
    • B01L3/02—Burettes; Pipettes
    • B01L3/0289—Apparatus for withdrawing or distributing predetermined quantities of fluid
    • B01L3/0293—Apparatus for withdrawing or distributing predetermined quantities of fluid for liquids
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2600/00—Oligonucleotides characterized by their use
    • C12Q2600/158—Expression markers
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N35/00—Automatic analysis not limited to methods or materials provided for in any single one of groups G01N1/00 - G01N33/00; Handling materials therefor
    • G01N2035/00465—Separating and mixing arrangements
    • G01N2035/00495—Centrifuges
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N35/00—Automatic analysis not limited to methods or materials provided for in any single one of groups G01N1/00 - G01N33/00; Handling materials therefor
    • G01N35/02—Automatic analysis not limited to methods or materials provided for in any single one of groups G01N1/00 - G01N33/00; Handling materials therefor using a plurality of sample containers moved by a conveyor system past one or more treatment or analysis stations
    • G01N35/04—Details of the conveyor system
    • G01N2035/0401—Sample carriers, cuvettes or reaction vessels
    • G01N2035/0403—Sample carriers with closing or sealing means
    • G01N2035/0405—Sample carriers with closing or sealing means manipulating closing or opening means, e.g. stoppers, screw caps, lids or covers
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N35/00—Automatic analysis not limited to methods or materials provided for in any single one of groups G01N1/00 - G01N33/00; Handling materials therefor
    • G01N35/10—Devices for transferring samples or any liquids to, in, or from, the analysis apparatus, e.g. suction devices, injection devices
    • G01N2035/1027—General features of the devices
    • G01N2035/103—General features of the devices using disposable tips

Landscapes

  • Chemical & Material Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Organic Chemistry (AREA)
  • Molecular Biology (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • General Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Biochemistry (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Immunology (AREA)
  • Analytical Chemistry (AREA)
  • Microbiology (AREA)
  • Biophysics (AREA)
  • Biotechnology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • Physics & Mathematics (AREA)
  • Genetics & Genomics (AREA)
  • Clinical Laboratory Science (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Apparatus Associated With Microorganisms And Enzymes (AREA)
  • Other Investigation Or Analysis Of Materials By Electrical Means (AREA)
  • Investigating Or Analysing Materials By The Use Of Chemical Reactions (AREA)

Abstract

L'invention concerne des procédés et dispositifs d'échantillonnage permettant d'analyser quantitativement des réactions d'amplification d'acide nucléique. Des informations quantitatives recueillies lors du déroulement d'un protocole d'amplification permettent de développer un profil d'amplification détaillé qui permet, à son tour, de déterminer de manière fiable la quantité de matrices initiales. Les procédés selon l'invention peuvent en particulier être utilisés pour analyser quantitativement l'expression de gènes ou unités de transcription multiples, non seulement lors de la même réaction d'amplification, mais aussi au cours de réactions d'amplification multiples.
CA002509769A 2002-11-21 2003-11-21 Procede et dispositif d'echantillonnage permettant d'analyser des reactions d'amplification Abandoned CA2509769A1 (fr)

Applications Claiming Priority (7)

Application Number Priority Date Filing Date Title
US42803802P 2002-11-21 2002-11-21
US60/428,038 2002-11-21
US43998203P 2003-01-14 2003-01-14
US44001003P 2003-01-14 2003-01-14
US60/439,982 2003-01-14
US60/440,010 2003-01-14
PCT/US2003/037420 WO2004048528A2 (fr) 2002-11-21 2003-11-21 Procede et dispositif d'echantillonnage permettant d'analyser des reactions d'amplification

Publications (1)

Publication Number Publication Date
CA2509769A1 true CA2509769A1 (fr) 2004-06-10

Family

ID=32397974

Family Applications (1)

Application Number Title Priority Date Filing Date
CA002509769A Abandoned CA2509769A1 (fr) 2002-11-21 2003-11-21 Procede et dispositif d'echantillonnage permettant d'analyser des reactions d'amplification

Country Status (6)

Country Link
EP (1) EP1563097A4 (fr)
JP (1) JP2006507849A (fr)
KR (1) KR20050075436A (fr)
AU (1) AU2003291144A1 (fr)
CA (1) CA2509769A1 (fr)
WO (1) WO2004048528A2 (fr)

Families Citing this family (8)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US20030077611A1 (en) 2001-10-24 2003-04-24 Sention Methods and systems for dynamic gene expression profiling
US7081339B2 (en) 2002-04-12 2006-07-25 Primera Biosystems, Inc. Methods for variation detection
US7445893B2 (en) 2002-04-12 2008-11-04 Primera Biosystems, Inc. Sampling method for amplification reaction analysis
JP4981295B2 (ja) * 2005-10-04 2012-07-18 キヤノン株式会社 Ｄｎａ処理方法およびｄｎａ処理装置
US20080003142A1 (en) * 2006-05-11 2008-01-03 Link Darren R Microfluidic devices
DE102013200193A1 (de) 2013-01-09 2014-07-10 Hamilton Bonaduz Ag Probenverarbeitungssystem mit Dosiervorrichtung und Thermocycler
KR101816288B1 (ko) * 2016-08-11 2018-01-09 한국기초과학지원연구원 고흡수성 수지를 이용한 시료의 전처리 방법 및 이를 위한 디바이스
JP7090256B1 (ja) 2022-01-14 2022-06-24 ヨダカ技研株式会社 温度調整装置、駆動装置、遺伝子増幅システム、遺伝子増幅方法、及び遺伝子増幅プログラム

Family Cites Families (9)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO1998033942A1 (fr) * 1997-01-30 1998-08-06 University Technology Corporation Diagnostic et traitement de defaillance myocardique
US6303305B1 (en) * 1999-03-30 2001-10-16 Roche Diagnostics, Gmbh Method for quantification of an analyte
CA2304260C (fr) * 1999-04-20 2009-03-24 Japan Bioindustry Association Methode pour determiner la concentration de molecules cibles d'acides nucleiques, capteurs a acides nucleiques pour cette methode et methode pour analyser les donnees ainsi obtenues
WO2000066995A2 (fr) * 1999-04-29 2000-11-09 Genome Therapeutics Corporation Dispositif permettant de traiter rapidement un echantillon d'adn par manipulation de liquide, thermocyclage et purification
US6618679B2 (en) * 2000-01-28 2003-09-09 Althea Technologies, Inc. Methods for analysis of gene expression
US6531282B1 (en) * 2000-05-30 2003-03-11 Oligotrail, Llc Multiplex amplification and analysis of selected STR loci
AU2001286784A1 (en) * 2000-08-25 2002-03-04 Genome Therapeutics Corporation Device for rapid dna sample processing with integrated liquid handling, thermocycling, and purification
US6482615B2 (en) * 2001-03-02 2002-11-19 Integrated Genetic Devices Ltd. Method and apparatus for effecting rapid thermal cycling of samples in microtiter plate size
US20030077611A1 (en) * 2001-10-24 2003-04-24 Sention Methods and systems for dynamic gene expression profiling

Also Published As

Publication number Publication date
KR20050075436A (ko) 2005-07-20
JP2006507849A (ja) 2006-03-09
EP1563097A4 (fr) 2006-03-15
EP1563097A2 (fr) 2005-08-17
WO2004048528A3 (fr) 2004-11-11
WO2004048528A2 (fr) 2004-06-10
AU2003291144A1 (en) 2004-06-18

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Legal Events

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