CA2488880A1 - Quinone-based therapeutic agent for hepatopathy - Google Patents
Quinone-based therapeutic agent for hepatopathy Download PDFInfo
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- CA2488880A1 CA2488880A1 CA002488880A CA2488880A CA2488880A1 CA 2488880 A1 CA2488880 A1 CA 2488880A1 CA 002488880 A CA002488880 A CA 002488880A CA 2488880 A CA2488880 A CA 2488880A CA 2488880 A1 CA2488880 A1 CA 2488880A1
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- menatetrenone
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K31/00—Medicinal preparations containing organic active ingredients
- A61K31/12—Ketones
- A61K31/122—Ketones having the oxygen directly attached to a ring, e.g. quinones, vitamin K1, anthralin
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
- A61P1/00—Drugs for disorders of the alimentary tract or the digestive system
- A61P1/16—Drugs for disorders of the alimentary tract or the digestive system for liver or gallbladder disorders, e.g. hepatoprotective agents, cholagogues, litholytics
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
- A61P35/00—Antineoplastic agents
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
- A61P43/00—Drugs for specific purposes, not provided for in groups A61P1/00-A61P41/00
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Abstract
To provide a remedy for a liver disease particularly by inhibiting the onset of portal-vein infusion (PVI), an excellent remedy/preventive for a liver disease containing menatetrenone as the active ingredient is disclosed. This remedy/preventive for a liver disease is efficacious against liver cancer, i n particular, DCP (des-.gamma.-carboxy prothrombin)-positive liver cancer. The above-described remedy/preventive for a liver disease containing menatetreno ne as the active ingredient exerts a remarkable effect of improving the prognos is following a therapy for liver cancer, thereby showing an excellent effect as a liver cancer recurrence inhibitor too.
Description
E 0 0 0 6 SW 0 6 ' CA 02488880 2004-12-08 SPECIFICATION
QUINONE-BASED THERAPEUTIC AGENT FOR HEPATOPATHY
Technical Field The present invention relates to an agent for treating hepatic disease, and more specifically to an agent for improving hepatic cancer prognosis, containing menatetrenoneas an activeingredient.
Background Art It is known that there is a high rate of portal venous invasion (hereinafter referred to as 'PVI') among hepatocellular carcinoma (hereinafter referred to as 'HCC' ) patients, and once PVI has occurred the prognosis is very poor. It is known that a high des-y-carboxy prothrombin (hereinafter referred to as 'DCP' ) level in HCC patients is closely linked to subsequent development of PVI (see Koike Y., Cancer 2001, 91 : 561-9) . DCP is also called PIVKA-II (protein induced by vitamin K absence or antagonist II ) . DCP is a pro thrombin that does not have normal coagulation activity, and is known to increase in level in the case of vitamin K (hereinafter referred to as 'VK' ) deficiency: DCP is thus a protein that is used as a marker for VK
deficiency or impaired VK absorption.
Moreover, it has been reported that if VK is administered to HCC patients with a high DCP level, then the serum DCP level drops (see Cancer 1992, 69:31-8), and that upon administering vitamin K-II (hereinafter referred to as 'VK-II') to a DCP-producing HCC
cell line in Vitro, cell proliferation is inhibited (see Hepatology 1995, 22:876-82).
However, until now no clinical data had been collected with regard to it being possible, by administering VK-II to patients after HCC treatment, to inhibit the occurrence of PVI, and to inhibit the recurrence of hepatocellular carcinoma, thus improving the prognosis.
In view of the above, it is an obj ect of the present invention to provide an excellent agent for treating or preventing hepatic disease.
Disclosure of Invention The present inventors accomplished the present invention by being the first to discover that administering an oral VK-II
preparation to DCP-producing HCC patientscontributesto inhibiting the occurrence of PVI after HCC treatment and improving the prognosis, and moreover inhibits the recurrence of hepatic cancer after treatment.
The above object is attained through an agent for treating or preventing hepatic disease containing menatetrenone as an active ingredient thereof.
According to a preferable aspect of the present invention, in the case of the above agent, the hepatic disease is hepatic cancer.
According to a preferable aspect of the present invention, in the case of the above agent, the hepatic cancer is des-y-carboxy prothrombin (DCP) positive hepatic cancer.
According to a preferable aspect of the present invention, the above agent improves prognosis after hepatic cancer treatment.
According to a preferable aspect of the present invention, the above agent is an agent that inhibits occurrence of portal venous invasion (PVI).
Moreover, the above obj ect is attained through an agent for inhibiting occurrence of portal venous invasion (PVI) containing menatetrenone as an active ingredient thereof.
Moreover, the above obj ect is attained through an agent for improving survival rate after hepatic cancer treatment containing menatetrenone as an active ingredient thereof.
Moreover, the above object is attained through an agent for inhibiting recurrence of hepatocellular carcinoma containing menatetrenone as an active ingredient thereof.
Moreover, the above object is attained through an agent for reducing DCP level containing menatetrenone as an active ingredient thereof.
Moreover, the above object is attained through a method of preventing portal venous invasion (PVI), comprising administering to a patient an effective dose of a medicine containing menatetrenone as an active ingredient thereof.
Moreover, the above object is attained through a method of inhibiting recurrence of hepatocellular carcinoma, comprising administering to a patient an effective dose of a medicine containing menatetrenone as an active ingredient thereof.
Moreover, the above object is attained through a method of regulating the level of DCP in the blood of a patient, comprising administering to the patient an effective dose of a medicine containing menatetrenone as an active ingredient thereof.
Moreover, the above object is attained through a use of menatetrenone for producing an agent for inhibiting occurrence of PVI.
Moreover, the above object is attained through a use of menatetrenone for inhibiting recurrence of hepatocellular carcinoma.
Furthermore, the above object is attained through an agent for treating or preventing hepatic disease containing a vitamin K as an active ingredient thereof.
The menatetrenone-containing agent for treating hepatic disease according to the present invention has an excellent effect of inhibiting occurrence of PVI with hepatic disease, in particular DCP positive hepatic cancer, and moreover has an excellent effect of improving the prognosis after hepatic cancer treatment.
Furthermore, the menatetrenone-containing agent for treating hepatic disease according to the present invention is very useful in inhibiting recurrence of hepatic cancer after treatment.
Brief Description of Drawings FIG. 1 shows a selection flowchart of the patient;
FIG. 2 shows a graph indicating changes in DCP level in serum;
FIG. 3 shows a graph indicating changes in PVI incidence rate;
FIG. 4 shows a graph indicating changes in survival rate;
FIG. 5 shows graph indicating the effect of VK-II
QUINONE-BASED THERAPEUTIC AGENT FOR HEPATOPATHY
Technical Field The present invention relates to an agent for treating hepatic disease, and more specifically to an agent for improving hepatic cancer prognosis, containing menatetrenoneas an activeingredient.
Background Art It is known that there is a high rate of portal venous invasion (hereinafter referred to as 'PVI') among hepatocellular carcinoma (hereinafter referred to as 'HCC' ) patients, and once PVI has occurred the prognosis is very poor. It is known that a high des-y-carboxy prothrombin (hereinafter referred to as 'DCP' ) level in HCC patients is closely linked to subsequent development of PVI (see Koike Y., Cancer 2001, 91 : 561-9) . DCP is also called PIVKA-II (protein induced by vitamin K absence or antagonist II ) . DCP is a pro thrombin that does not have normal coagulation activity, and is known to increase in level in the case of vitamin K (hereinafter referred to as 'VK' ) deficiency: DCP is thus a protein that is used as a marker for VK
deficiency or impaired VK absorption.
Moreover, it has been reported that if VK is administered to HCC patients with a high DCP level, then the serum DCP level drops (see Cancer 1992, 69:31-8), and that upon administering vitamin K-II (hereinafter referred to as 'VK-II') to a DCP-producing HCC
cell line in Vitro, cell proliferation is inhibited (see Hepatology 1995, 22:876-82).
However, until now no clinical data had been collected with regard to it being possible, by administering VK-II to patients after HCC treatment, to inhibit the occurrence of PVI, and to inhibit the recurrence of hepatocellular carcinoma, thus improving the prognosis.
In view of the above, it is an obj ect of the present invention to provide an excellent agent for treating or preventing hepatic disease.
Disclosure of Invention The present inventors accomplished the present invention by being the first to discover that administering an oral VK-II
preparation to DCP-producing HCC patientscontributesto inhibiting the occurrence of PVI after HCC treatment and improving the prognosis, and moreover inhibits the recurrence of hepatic cancer after treatment.
The above object is attained through an agent for treating or preventing hepatic disease containing menatetrenone as an active ingredient thereof.
According to a preferable aspect of the present invention, in the case of the above agent, the hepatic disease is hepatic cancer.
According to a preferable aspect of the present invention, in the case of the above agent, the hepatic cancer is des-y-carboxy prothrombin (DCP) positive hepatic cancer.
According to a preferable aspect of the present invention, the above agent improves prognosis after hepatic cancer treatment.
According to a preferable aspect of the present invention, the above agent is an agent that inhibits occurrence of portal venous invasion (PVI).
Moreover, the above obj ect is attained through an agent for inhibiting occurrence of portal venous invasion (PVI) containing menatetrenone as an active ingredient thereof.
Moreover, the above obj ect is attained through an agent for improving survival rate after hepatic cancer treatment containing menatetrenone as an active ingredient thereof.
Moreover, the above object is attained through an agent for inhibiting recurrence of hepatocellular carcinoma containing menatetrenone as an active ingredient thereof.
Moreover, the above object is attained through an agent for reducing DCP level containing menatetrenone as an active ingredient thereof.
Moreover, the above object is attained through a method of preventing portal venous invasion (PVI), comprising administering to a patient an effective dose of a medicine containing menatetrenone as an active ingredient thereof.
Moreover, the above object is attained through a method of inhibiting recurrence of hepatocellular carcinoma, comprising administering to a patient an effective dose of a medicine containing menatetrenone as an active ingredient thereof.
Moreover, the above object is attained through a method of regulating the level of DCP in the blood of a patient, comprising administering to the patient an effective dose of a medicine containing menatetrenone as an active ingredient thereof.
Moreover, the above object is attained through a use of menatetrenone for producing an agent for inhibiting occurrence of PVI.
Moreover, the above object is attained through a use of menatetrenone for inhibiting recurrence of hepatocellular carcinoma.
Furthermore, the above object is attained through an agent for treating or preventing hepatic disease containing a vitamin K as an active ingredient thereof.
The menatetrenone-containing agent for treating hepatic disease according to the present invention has an excellent effect of inhibiting occurrence of PVI with hepatic disease, in particular DCP positive hepatic cancer, and moreover has an excellent effect of improving the prognosis after hepatic cancer treatment.
Furthermore, the menatetrenone-containing agent for treating hepatic disease according to the present invention is very useful in inhibiting recurrence of hepatic cancer after treatment.
Brief Description of Drawings FIG. 1 shows a selection flowchart of the patient;
FIG. 2 shows a graph indicating changes in DCP level in serum;
FIG. 3 shows a graph indicating changes in PVI incidence rate;
FIG. 4 shows a graph indicating changes in survival rate;
FIG. 5 shows graph indicating the effect of VK-II
administration on hepatic cancer recurrence inhibition (500 recurrence);
FIG. 6 shows a graph indicating results for HCV cases only in the trial to verify the effect of VK-II administration on hepatic cancer recurrence inhibition (50o recurrence);
FIG. 7 shows a graph indicating results in the case of excluding cases of local recurrence in the trial to verify the effect of VK-II
administration on hepatic cancer recurrence inhibition (500 recurrence);
FIG. 8 shows a graph indicating results in the case of excluding cases of recurrence within 6 months in the trial to verify the effect of VK-II administration on hepatic cancer recurrence inhibition (50o recurrence);
FIG. 9 shows a diagram indicating results of analyzing the risk ratio (RR) for recurrence using a Cox proportional hazardmodel;
and FIG. 10 shows graphs indicating results of analyzing the DCP
level before treatment and upon recurrence.
Best Mode for Carrying Out the Invention Following is a more detailed description of the present invention by way of examples; however, the present invention is not limited to these examples.
Hepatic cancer occurs with a high rate from chronic hepatitis and cirrhosis, which are targeted by the present invention, and having occurred hepatic cancer reoccurs after treatment with a high 11 ' E 0 0 0 6 SWO 6 CA 02488880 2004-12-08 rate. For example, there are cases of cirrhosis developing from type C hepatitis or type B hepatitis, and there being recurrence after the tumors have been excised. According to the agent for treating hepatic disease of the present invention, the prognosis after such hepatic cancer treatment can be improved very effectively (i.e. recurrence can be prevented or treated). Moreover, the occurrence of PVI, which is one form of recurrence of hepatic cancer with poor prognosis, can be inhibited very effectively.
The menatetrenone used in the present invention has the chemical name 2-methyl-3-tetraprenyl-1,4-naphthoquinone, and has a structural formula as shown below.
O
Menatetrenone is a yellow crystalline or oily substance, has no taste or odor, and is readily decomposed by light. Moreover, menatetrenone hardly dissolves in water. Menatetrenone is also called vitamin K-II (VK-II), and regarding the pharmacological action thereof, in the process of protein synthesis of blood coagulation factors (prothrombin, VII, IX, X), menatetrenone participates in the carboxylation reaction when glutamic acid residues are converted into physiologically active y-carboxyglutamic acid, and menatetrenonepromotes synthesis in the liver of normal prothrombin and so on, and activates the hemostasis mechanism in a living body, thus physiologically realizing hemostasis.
The menatetrenone that is the active ingredient in the medicine according to the present invention may be in the form of an anhydride or a hydrate. Moreover, menatetrenone has crystal polymorphs, but there is no limitation, with it being possible for the menatetrenone to be in any one of the crystal forms, or a mixture thereof.
Furthermore, a metabolite produced through the menatetrenone according to the present invention being decomposed in a living body is also included in the scope of the claims of the present invention.
The menatetrenone used in the present invention can be produced using a publicly known method, and as a representative example, can easily be produced using the method disclosed in Japanese Patent Application Laid-open No. 49-55650; alternatively, the menatetrenone can easily be procured from a chemical manufacturer.
Moreover, the menatetrenone can be procured as a pharmaceutical preparation such as a capsule or an inj ection . Regarding the medicine of the present invention, the menatetrenone may be used as is, or may be made into a pharmaceutical preparation using a commonly used method by blending with ingredients that are commonly used as raw materials of medicinal preparations such as publicly known pharmaceutically permissible carriers and so on (e. g. excipients, binders, disintegrators, lubricants, colorants, flavorings, stabilizers, emulsifiers, absorption promoters, surfactants, pH
regulators, preservatives, antioxidants, etc.). Moreover, other ingredients such as vitamins and amino acids may be blended in as required. Examples of the form of the pharmaceutical preparation are tablets, a powder, granules, capsules, a syrup, suppositories, an injection, an ointment, a poultice, and so on.
Moreover, in the present invention, there are no particular limitations on the form of administration of the menatetrenone, although oraladministrationispreferable. Menatetrenonecapsules can be procured as Kaytwo capsules (proprietary name, made by Eisai Co., Ltd.) and Glakay capsules (proprietary name, made by Eisai Co . , Ltd. ) , a menatetrenone syrup can be procured as Kaytwo syrup (proprietary name, made by Eisai Co., Ltd.), and an injection can be procured as Kaytwo N (proprietary name, made by Eisai Co., Ltd. ) .
The menatetrenone-containing medicine according to the present invention is useful for treating or preventing hepatic disease. A preferable dose of the menatetrenone is generally 10 to 200 mg/day, more preferably 30 to 135 mg/day.
Examples Trial examples of the present invention are given below;
however, these trial examples are merely illustrative, and the present invention is not limited thereto. A person skilled in the art could implement the present invention not only through the trial examples shown below, but also with any of various modifications within the scope of the claims appended to the specification of the present application, and such modifications are deemed to be included in the scope of the claims of the present application.
FIG. 6 shows a graph indicating results for HCV cases only in the trial to verify the effect of VK-II administration on hepatic cancer recurrence inhibition (50o recurrence);
FIG. 7 shows a graph indicating results in the case of excluding cases of local recurrence in the trial to verify the effect of VK-II
administration on hepatic cancer recurrence inhibition (500 recurrence);
FIG. 8 shows a graph indicating results in the case of excluding cases of recurrence within 6 months in the trial to verify the effect of VK-II administration on hepatic cancer recurrence inhibition (50o recurrence);
FIG. 9 shows a diagram indicating results of analyzing the risk ratio (RR) for recurrence using a Cox proportional hazardmodel;
and FIG. 10 shows graphs indicating results of analyzing the DCP
level before treatment and upon recurrence.
Best Mode for Carrying Out the Invention Following is a more detailed description of the present invention by way of examples; however, the present invention is not limited to these examples.
Hepatic cancer occurs with a high rate from chronic hepatitis and cirrhosis, which are targeted by the present invention, and having occurred hepatic cancer reoccurs after treatment with a high 11 ' E 0 0 0 6 SWO 6 CA 02488880 2004-12-08 rate. For example, there are cases of cirrhosis developing from type C hepatitis or type B hepatitis, and there being recurrence after the tumors have been excised. According to the agent for treating hepatic disease of the present invention, the prognosis after such hepatic cancer treatment can be improved very effectively (i.e. recurrence can be prevented or treated). Moreover, the occurrence of PVI, which is one form of recurrence of hepatic cancer with poor prognosis, can be inhibited very effectively.
The menatetrenone used in the present invention has the chemical name 2-methyl-3-tetraprenyl-1,4-naphthoquinone, and has a structural formula as shown below.
O
Menatetrenone is a yellow crystalline or oily substance, has no taste or odor, and is readily decomposed by light. Moreover, menatetrenone hardly dissolves in water. Menatetrenone is also called vitamin K-II (VK-II), and regarding the pharmacological action thereof, in the process of protein synthesis of blood coagulation factors (prothrombin, VII, IX, X), menatetrenone participates in the carboxylation reaction when glutamic acid residues are converted into physiologically active y-carboxyglutamic acid, and menatetrenonepromotes synthesis in the liver of normal prothrombin and so on, and activates the hemostasis mechanism in a living body, thus physiologically realizing hemostasis.
The menatetrenone that is the active ingredient in the medicine according to the present invention may be in the form of an anhydride or a hydrate. Moreover, menatetrenone has crystal polymorphs, but there is no limitation, with it being possible for the menatetrenone to be in any one of the crystal forms, or a mixture thereof.
Furthermore, a metabolite produced through the menatetrenone according to the present invention being decomposed in a living body is also included in the scope of the claims of the present invention.
The menatetrenone used in the present invention can be produced using a publicly known method, and as a representative example, can easily be produced using the method disclosed in Japanese Patent Application Laid-open No. 49-55650; alternatively, the menatetrenone can easily be procured from a chemical manufacturer.
Moreover, the menatetrenone can be procured as a pharmaceutical preparation such as a capsule or an inj ection . Regarding the medicine of the present invention, the menatetrenone may be used as is, or may be made into a pharmaceutical preparation using a commonly used method by blending with ingredients that are commonly used as raw materials of medicinal preparations such as publicly known pharmaceutically permissible carriers and so on (e. g. excipients, binders, disintegrators, lubricants, colorants, flavorings, stabilizers, emulsifiers, absorption promoters, surfactants, pH
regulators, preservatives, antioxidants, etc.). Moreover, other ingredients such as vitamins and amino acids may be blended in as required. Examples of the form of the pharmaceutical preparation are tablets, a powder, granules, capsules, a syrup, suppositories, an injection, an ointment, a poultice, and so on.
Moreover, in the present invention, there are no particular limitations on the form of administration of the menatetrenone, although oraladministrationispreferable. Menatetrenonecapsules can be procured as Kaytwo capsules (proprietary name, made by Eisai Co., Ltd.) and Glakay capsules (proprietary name, made by Eisai Co . , Ltd. ) , a menatetrenone syrup can be procured as Kaytwo syrup (proprietary name, made by Eisai Co., Ltd.), and an injection can be procured as Kaytwo N (proprietary name, made by Eisai Co., Ltd. ) .
The menatetrenone-containing medicine according to the present invention is useful for treating or preventing hepatic disease. A preferable dose of the menatetrenone is generally 10 to 200 mg/day, more preferably 30 to 135 mg/day.
Examples Trial examples of the present invention are given below;
however, these trial examples are merely illustrative, and the present invention is not limited thereto. A person skilled in the art could implement the present invention not only through the trial examples shown below, but also with any of various modifications within the scope of the claims appended to the specification of the present application, and such modifications are deemed to be included in the scope of the claims of the present application.
Trial Example 1 A clinical trial (randomized prospective controlled study) was carried out as follows.
Out of patients with hepatocellular carcinoma, ones having a serum DCP level above 60 IU/L (DCP positive hepatic cancer) were included in the trial. On the other hand, patients having portal venous invasion, and patients in which there was alreadyVKmetabolism through administration of VK or an anti-VK agent, were excluded from the trial. The details of the trial were as shown in Table 1 .
Table 1 Trial subjects Subjects included 1. Hepatic cancer patients 2. Serum DCP level >_ 60 IU/L
Subjects excluded 1. Portal venous invasion 2. Cancer metastasize out of liver 3. uncontrolled ascites 4. Bilirubin > 3.0 mg/dl 5. Taking vitamin K preparation, warfarin Group administered VK-II
Took 45 mg of vitamin K-II (Glakay) three times after hepatic cancer treatment Group not administered VK-II
Out of patients with hepatocellular carcinoma, ones having a serum DCP level above 60 IU/L (DCP positive hepatic cancer) were included in the trial. On the other hand, patients having portal venous invasion, and patients in which there was alreadyVKmetabolism through administration of VK or an anti-VK agent, were excluded from the trial. The details of the trial were as shown in Table 1 .
Table 1 Trial subjects Subjects included 1. Hepatic cancer patients 2. Serum DCP level >_ 60 IU/L
Subjects excluded 1. Portal venous invasion 2. Cancer metastasize out of liver 3. uncontrolled ascites 4. Bilirubin > 3.0 mg/dl 5. Taking vitamin K preparation, warfarin Group administered VK-II
Took 45 mg of vitamin K-II (Glakay) three times after hepatic cancer treatment Group not administered VK-II
Hepatic cancer treatment only Judgement criteria 1. Occurrence of portal venous invasion 2. Death FIG. 1 shows a selection flowchart of the patient. 126 hepatic cancer patients were given treatment from February 1999 to November 2001. As the hepatic cancer treatment, percutaneous cauterization therapy (RFA and/or PEIT) for HCC, treatment via the blood vessels (TAE or TAI) , or surgical excision was carried out. Of the patients, 5 were excluded from the present trial.
Next, the remaining 121 patients were randomly divided into a treated group (n = 60 ) and an untreated group (n = 61 ) . The treated group were orally administered 45 mg/day of VK-II (proprietary name Glakay, made by Eisai Co., Ltd. ) after the hepatic cancer treatment, while the untreated group were not administered VK-II.
Follow-up tests were carried out after the hepatic cancer treatment. As the follow-up tests, ultrasonography (abdominal echography) was carried out every 3 months, a CT scan was carried out every 6 months, and the levels of the tumor markers alpha-fetoprotein and DCP were measured every one month.
Table 2 shows the profile of the patients. No important differences were found in any of the clinical parameters between the treated group and the untreated group.
Table 2 Patients Profile Treated group Untreated group P
(n = 60) (n=61) Age 66.97.0 67.37.5 .8 Sex (male/female) 36/24 45/16 .12 Virus (HCV/non HCV) 50/10 52/9 .81 Tumor size (mm) 3211 3518 .27 Number of tumors 4.03.2 4.33.5 .66 Child class (A/B or C) 18/42 27/34 .13 Albumin (g/dl) 3.40.5 3.50.5 .3 Bilirubin (mg/dl) 1.20.7 1.10.9 .4 ALT (IU/L) 5538 6147 .47 Prothrombin (o) 7816 7814 .99 Blood platelets (10q/mm3)10. 86. 0 11.56. 6 .
AFP (ng/L) 26687666 15397036 .42 DCP (IU/L) 9852639 11785108 .80 PTA with/without 48/12 41/20 .15 average ~ SD (Median) FIG. 2 shows a graph indicating changes in the DCP level in the serum. The solid line is for the treated group, and the dashed line is for the untreated group. For both the treated group and the untreated group, the DCP level dropped after the hepatic cancer treatment. Subsequently, the DCP level remained approximately constant for 12 months for the treated group, whereas the DCP level gradually increased for the untreated group.
FIG. 3 shows a graph indicating changes in the PVI incidence rate. As shown in FIG. 3, for the treated group, the PVI incidence rate was 2 o after 1 year, and 23 o after 2 years . On the other hand, for the untreated group, the PVI incidence rate was 23o after 1 year, and 47o after 2 years (P = 0.018).
FIG. 4 shows a graph indicating changes in the survival rate.
As shown in FIG. 4, the survival rate after 2 years was 66~ for the treated group, but 28o for the untreated group (P = 0.044).
Statistical analysis was carried out on the PVI incidence rate S w 0 6 CA 02488880 2004-12-08 and the survival rate for the two groups. That is, calculations were carried out using the Cox proportional hazard model, and a test was carried out using the log-rank method. The average observation period was 12 ~ 8 months.
From the above results, it is suggested that by orally administering a VK-II preparation, incidence of PVI in DCP positive HCC patients is suppressed very effectively, and moreover survival rate is greatly increased, and hence the prognosis after hepatic cancer treatment is markedly improved.
Trial Example 2 A trial was carried out as follows with an objective of investigating the effect and safety of VK-II in inhibiting recurrence of hepatocellular carcinoma after treatment.
61 cases in which hepatocellular carcinoma was diagnosed and then after treatment therefor necrosis (or curative excision) was judged by contrast CT to have completely occurred were entered from March 1999 to March 2001, the entered cases were divided into two groups, namely a VK-II-administered group for which the end of the patient ID number was an odd number and a non-administered group (control group) for which the end of the patient ID number was an even number, and a VK-II preparation (proprietary name Glakay, made by Eisai Co., Ltd.) was orally administered at a dose of 45 mg/day to the administered group. Contrast CT or MRI was carried out every 3 months, and a statistical analysis of the time period until recurrence was carried out. Specifically, comparison was carried out using the Kaplan-Meier method (log-rank test), and the risk ratio for recurrence was analyzed using the Cox proportional hazard model.
As shown in Table 3, the average observation period for the 61 entered cases (32 cases in the administered group, 29 cases in the non-administered group) was 19.6 months (7-32).
Table 3 Administered Control Subjects group(32 cases) group(29 cases) Age 63.37.5(48-75) 64.56.7(45-74) Sex (male/female) 23/9 18/11 Cause of disease 28/3/1 26/2/1 (type C/type B/type B+C) History of alcohol consumption 10/22 3/26 (addict/non-addict) First occurrence/recurrence 15/17 14/15 Tumor size (mm) 17.75.1(10-30) 19.46.9(10-38) Number of tumors 1.500.88(1-4) 1.480.74(1-3) Log AFP (ng/ml) 1. 470.61 1. 720.91 (0.60-3.09) (0.48-3.88) PIVKA-II (mAU/ml) 41.865.4 70.3104.1 (8-346) (7-417) Liver function (LD A/B/C) 15/16/1 13/15/1 Treatment method 1/31 3/26 (excision/non-excision) Average observation period 24.37.1(13-37) 24.28.3(12-37) (months) Upon calculating cumulative recurrence rates, the 1-year recurrence rate was found to be 12.50 for the VK-II-administered group and 55.20 for the control group, and the 2-year recurrence rate was found to be 39.60 for the VK-II-administered group and 85 . 5 o for the control group . From these results, it was found that the hepatic cancer cumulative recurrence rate was statistically significantly suppressedfor the VK-II-administered group compared with the control group.
FIG. 5 shows a graph indicating the effect of VK-II
administration on hepatic cancer recurrence inhibition (500 recurrence ) . As shown in FIG . 5, the time period until 50 o recurrence was 26 months for the VK-II-administered group, but was 10 months for the control group.
Moreover, upon calculating the hepatic cancer cumulative recurrence rates considering only HCV cases ( type C hepatitis cases ) , the 1-year recurrence rate was found to be 7.1o for the VK-II-administered group and 61 . 5 o for the control group, and the 2-year recurrence rate was found to be 37.80 for the VK-II-administered group and87.2%for thecontrolgroup. From these results, it was found that, even when considering only HCV cases, the hepatic cancer cumulative recurrence rate was statistically significantlysuppressedfor the VK-II-administered group compared with the control group.
FIG. 6 shows a graph indicating the results for the HCV cases only in the trial to verify the effect of VK-II administration on hepatic cancer recurrence inhibition (50o recurrence). As shown in FIG. 6, the time period until 50o recurrence was 26 months for the VK-II-administered group, but was 10 months for the control group.
FIG. 9 shows a diagram indicating the results of analyzing the risk ratio (RR) for recurrence using the Cox proportional hazard model. As shown in FIG. 9, taking the risk ratio for recurrence of hepatic cancer to be 1 for the control group, this risk ratio is approximately one thirdof that at 0. 329 for theVK-II-administered group; in particular, considering only the HCV cases, the risk ratio becomes 0.210, i.e. is reduced to approximately one fifth, by administering VK-TI.
FIG. 7 shows a graph indicating the results in the case of excluding cases of local recurrence in the trial to verify the effect of VK-II administration on hepatic cancer recurrence inhibition (50o recurrence) (VK-II-administered group: 29 cases, non-administered group: 22 cases) . Moreover, FIG. 8 shows a graph indicating the results in the case of excluding cases of recurrence within 6 months in the trial to verify the effect of VK-II
administration on hepatic cancer recurrence inhibition (500 recurrence) (VK-II-administered group: 31 cases, non-administered group: 22 cases) . As shown in FIGS. 7 and 8, in these cases, again the hepatic cancer cumulative recurrence rate was statistically significantlysuppressedfor the VK-II-administered group compared with the control group.
FIG. 10 shows graphs indicating the results of analyzing the DCP level before treatment and upon recurrence. As shown in FIG.
Next, the remaining 121 patients were randomly divided into a treated group (n = 60 ) and an untreated group (n = 61 ) . The treated group were orally administered 45 mg/day of VK-II (proprietary name Glakay, made by Eisai Co., Ltd. ) after the hepatic cancer treatment, while the untreated group were not administered VK-II.
Follow-up tests were carried out after the hepatic cancer treatment. As the follow-up tests, ultrasonography (abdominal echography) was carried out every 3 months, a CT scan was carried out every 6 months, and the levels of the tumor markers alpha-fetoprotein and DCP were measured every one month.
Table 2 shows the profile of the patients. No important differences were found in any of the clinical parameters between the treated group and the untreated group.
Table 2 Patients Profile Treated group Untreated group P
(n = 60) (n=61) Age 66.97.0 67.37.5 .8 Sex (male/female) 36/24 45/16 .12 Virus (HCV/non HCV) 50/10 52/9 .81 Tumor size (mm) 3211 3518 .27 Number of tumors 4.03.2 4.33.5 .66 Child class (A/B or C) 18/42 27/34 .13 Albumin (g/dl) 3.40.5 3.50.5 .3 Bilirubin (mg/dl) 1.20.7 1.10.9 .4 ALT (IU/L) 5538 6147 .47 Prothrombin (o) 7816 7814 .99 Blood platelets (10q/mm3)10. 86. 0 11.56. 6 .
AFP (ng/L) 26687666 15397036 .42 DCP (IU/L) 9852639 11785108 .80 PTA with/without 48/12 41/20 .15 average ~ SD (Median) FIG. 2 shows a graph indicating changes in the DCP level in the serum. The solid line is for the treated group, and the dashed line is for the untreated group. For both the treated group and the untreated group, the DCP level dropped after the hepatic cancer treatment. Subsequently, the DCP level remained approximately constant for 12 months for the treated group, whereas the DCP level gradually increased for the untreated group.
FIG. 3 shows a graph indicating changes in the PVI incidence rate. As shown in FIG. 3, for the treated group, the PVI incidence rate was 2 o after 1 year, and 23 o after 2 years . On the other hand, for the untreated group, the PVI incidence rate was 23o after 1 year, and 47o after 2 years (P = 0.018).
FIG. 4 shows a graph indicating changes in the survival rate.
As shown in FIG. 4, the survival rate after 2 years was 66~ for the treated group, but 28o for the untreated group (P = 0.044).
Statistical analysis was carried out on the PVI incidence rate S w 0 6 CA 02488880 2004-12-08 and the survival rate for the two groups. That is, calculations were carried out using the Cox proportional hazard model, and a test was carried out using the log-rank method. The average observation period was 12 ~ 8 months.
From the above results, it is suggested that by orally administering a VK-II preparation, incidence of PVI in DCP positive HCC patients is suppressed very effectively, and moreover survival rate is greatly increased, and hence the prognosis after hepatic cancer treatment is markedly improved.
Trial Example 2 A trial was carried out as follows with an objective of investigating the effect and safety of VK-II in inhibiting recurrence of hepatocellular carcinoma after treatment.
61 cases in which hepatocellular carcinoma was diagnosed and then after treatment therefor necrosis (or curative excision) was judged by contrast CT to have completely occurred were entered from March 1999 to March 2001, the entered cases were divided into two groups, namely a VK-II-administered group for which the end of the patient ID number was an odd number and a non-administered group (control group) for which the end of the patient ID number was an even number, and a VK-II preparation (proprietary name Glakay, made by Eisai Co., Ltd.) was orally administered at a dose of 45 mg/day to the administered group. Contrast CT or MRI was carried out every 3 months, and a statistical analysis of the time period until recurrence was carried out. Specifically, comparison was carried out using the Kaplan-Meier method (log-rank test), and the risk ratio for recurrence was analyzed using the Cox proportional hazard model.
As shown in Table 3, the average observation period for the 61 entered cases (32 cases in the administered group, 29 cases in the non-administered group) was 19.6 months (7-32).
Table 3 Administered Control Subjects group(32 cases) group(29 cases) Age 63.37.5(48-75) 64.56.7(45-74) Sex (male/female) 23/9 18/11 Cause of disease 28/3/1 26/2/1 (type C/type B/type B+C) History of alcohol consumption 10/22 3/26 (addict/non-addict) First occurrence/recurrence 15/17 14/15 Tumor size (mm) 17.75.1(10-30) 19.46.9(10-38) Number of tumors 1.500.88(1-4) 1.480.74(1-3) Log AFP (ng/ml) 1. 470.61 1. 720.91 (0.60-3.09) (0.48-3.88) PIVKA-II (mAU/ml) 41.865.4 70.3104.1 (8-346) (7-417) Liver function (LD A/B/C) 15/16/1 13/15/1 Treatment method 1/31 3/26 (excision/non-excision) Average observation period 24.37.1(13-37) 24.28.3(12-37) (months) Upon calculating cumulative recurrence rates, the 1-year recurrence rate was found to be 12.50 for the VK-II-administered group and 55.20 for the control group, and the 2-year recurrence rate was found to be 39.60 for the VK-II-administered group and 85 . 5 o for the control group . From these results, it was found that the hepatic cancer cumulative recurrence rate was statistically significantly suppressedfor the VK-II-administered group compared with the control group.
FIG. 5 shows a graph indicating the effect of VK-II
administration on hepatic cancer recurrence inhibition (500 recurrence ) . As shown in FIG . 5, the time period until 50 o recurrence was 26 months for the VK-II-administered group, but was 10 months for the control group.
Moreover, upon calculating the hepatic cancer cumulative recurrence rates considering only HCV cases ( type C hepatitis cases ) , the 1-year recurrence rate was found to be 7.1o for the VK-II-administered group and 61 . 5 o for the control group, and the 2-year recurrence rate was found to be 37.80 for the VK-II-administered group and87.2%for thecontrolgroup. From these results, it was found that, even when considering only HCV cases, the hepatic cancer cumulative recurrence rate was statistically significantlysuppressedfor the VK-II-administered group compared with the control group.
FIG. 6 shows a graph indicating the results for the HCV cases only in the trial to verify the effect of VK-II administration on hepatic cancer recurrence inhibition (50o recurrence). As shown in FIG. 6, the time period until 50o recurrence was 26 months for the VK-II-administered group, but was 10 months for the control group.
FIG. 9 shows a diagram indicating the results of analyzing the risk ratio (RR) for recurrence using the Cox proportional hazard model. As shown in FIG. 9, taking the risk ratio for recurrence of hepatic cancer to be 1 for the control group, this risk ratio is approximately one thirdof that at 0. 329 for theVK-II-administered group; in particular, considering only the HCV cases, the risk ratio becomes 0.210, i.e. is reduced to approximately one fifth, by administering VK-TI.
FIG. 7 shows a graph indicating the results in the case of excluding cases of local recurrence in the trial to verify the effect of VK-II administration on hepatic cancer recurrence inhibition (50o recurrence) (VK-II-administered group: 29 cases, non-administered group: 22 cases) . Moreover, FIG. 8 shows a graph indicating the results in the case of excluding cases of recurrence within 6 months in the trial to verify the effect of VK-II
administration on hepatic cancer recurrence inhibition (500 recurrence) (VK-II-administered group: 31 cases, non-administered group: 22 cases) . As shown in FIGS. 7 and 8, in these cases, again the hepatic cancer cumulative recurrence rate was statistically significantlysuppressedfor the VK-II-administered group compared with the control group.
FIG. 10 shows graphs indicating the results of analyzing the DCP level before treatment and upon recurrence. As shown in FIG.
10, with all of the cases of recurrence in the VK-II-administered group, DCP was negative, and moreover there were no side effects, and there were no dropout.
The action of VK-II according to the present application with regard to invasion/metastasis of hepatic cancer cells was studied in vitro. The action on invasion ability was investigated through an invasion assay using HepG2 cells and a Matrigel chamber. The results were that it was found that the number of cells passing P~ ~ ~
through the Matrigel dropped upon adding VK-II, with the drop depending on the concentration of the VK-II. Regarding the action on the metastasis ability, the action of VK-II on the expression of extracellular matrix metalloproteinases (MMPs) was investigated using the Western blot method. Upon investigating the MMP-1 and MMP-3 protein expression in the case of adding VK-II to hepatic cancer cells, it was found that the expression was inhibited. From the above, although the data is in vitro, it can be presumed that VK-II inhibits invasion/metastasis of hepatic cancer cells.
Industrial Applicability As described above, the menatetrenone-containing agent for treating or preventing hepatic disease according to the present invention has an excellent effect of inhibiting occurrence of PVI
with hepatic disease, in particular DCP positive hepatic cancer, and moreover has an excellent effect of improving the prognosis after hepatic cancer treatment.
Furthermore, the menatetrenone-containing agentfor treating hepatic disease according to the present invention is very useful in inhibiting recurrence of hepatic cancer after treatment.
The action of VK-II according to the present application with regard to invasion/metastasis of hepatic cancer cells was studied in vitro. The action on invasion ability was investigated through an invasion assay using HepG2 cells and a Matrigel chamber. The results were that it was found that the number of cells passing P~ ~ ~
through the Matrigel dropped upon adding VK-II, with the drop depending on the concentration of the VK-II. Regarding the action on the metastasis ability, the action of VK-II on the expression of extracellular matrix metalloproteinases (MMPs) was investigated using the Western blot method. Upon investigating the MMP-1 and MMP-3 protein expression in the case of adding VK-II to hepatic cancer cells, it was found that the expression was inhibited. From the above, although the data is in vitro, it can be presumed that VK-II inhibits invasion/metastasis of hepatic cancer cells.
Industrial Applicability As described above, the menatetrenone-containing agent for treating or preventing hepatic disease according to the present invention has an excellent effect of inhibiting occurrence of PVI
with hepatic disease, in particular DCP positive hepatic cancer, and moreover has an excellent effect of improving the prognosis after hepatic cancer treatment.
Furthermore, the menatetrenone-containing agentfor treating hepatic disease according to the present invention is very useful in inhibiting recurrence of hepatic cancer after treatment.
Claims (15)
1. An agent for treating or preventing hepatic disease containing menatetrenone as an active ingredient thereof.
2. The agent according to claim 1, wherein the hepatic disease is hepatic cancer.
3. The agent according to claim 2, wherein the hepatic cancer is des-.gamma.-carboxy prothrombin positive hepatic cancer.
4. The agent according to any one of claims 1 through 3, wherein the agent improves prognosis after hepatic cancer treatment.
5. The agent according to claim 4, wherein the agent is an agent that inhibits occurrence of portal venous invasion.
6. An agent for inhibiting occurrence of portal venous invasion containing menatetrenone as an active ingredient thereof.
7 . An agent for improving survival rate after hepatic cancer treatment containing menatetrenone as an active ingredient thereof.
8. An agent for inhibiting recurrence of hepatocellular carcinoma containing menatetrenone as an active ingredient thereof.
9. An agent for reducing des-.gamma.-carboxy prothrombin level containing menatetrenone as an active ingredient thereof.
10. A method of preventing portal venous invasion, comprising administering to a patient an effective dose of a medicine containing menatetrenone as an active ingredient thereof.
11. A method of inhibiting recurrence of hepatocellular carcinoma, comprising administering to a patient an effective dose of a medicine containing menatetrenone as an active ingredient thereof.
12. A method of modulating the level of des-.gamma.-carboxy prothrombin in the blood of a patient, comprising administering to the patient an effective dose of a medicine containing menatetrenone as an active ingredient thereof.
13. A use of menatetrenone for manufacturing an agent for inhibiting occurrence of portal venous invasion.
14. A use of menatetrenone for inhibiting recurrence of hepatocellular carcinoma.
15. An agent for treating or preventing hepatic disease containing a vitamin K as an active ingredient thereof.
Applications Claiming Priority (11)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| JP2002172133 | 2002-06-12 | ||
| JP2002-172133 | 2002-06-12 | ||
| JP2002172162 | 2002-06-13 | ||
| JP2002-172162 | 2002-06-13 | ||
| JP2002-204709 | 2002-07-12 | ||
| JP2002204709A JP2004067513A (en) | 2002-06-12 | 2002-07-12 | Quinone-based therapeutic agent for hepatic disease |
| JP2002-245178 | 2002-08-26 | ||
| JP2002245178 | 2002-08-26 | ||
| PCT/JP2002/009640 WO2003105818A1 (en) | 2002-06-12 | 2002-09-19 | Quinone-based remedies for liver diseases |
| JPPCT/JP02/09640 | 2002-09-19 | ||
| PCT/JP2003/007491 WO2003105819A1 (en) | 2002-06-12 | 2003-06-12 | Quinone-type remedy for liver disease |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CA2488880A1 true CA2488880A1 (en) | 2003-12-24 |
Family
ID=29741087
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CA002488880A Abandoned CA2488880A1 (en) | 2002-06-12 | 2003-06-12 | Quinone-based therapeutic agent for hepatopathy |
Country Status (4)
| Country | Link |
|---|---|
| AU (1) | AU2003277184B2 (en) |
| BR (1) | BR0311781A (en) |
| CA (1) | CA2488880A1 (en) |
| WO (1) | WO2003105819A1 (en) |
Families Citing this family (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| MXPA06007734A (en) * | 2004-01-06 | 2007-01-26 | Eisai R&D Man Co Ltd | Preventive for the onset of liver cancer comprising quinone compound as the active ingredient. |
| WO2005077351A1 (en) * | 2004-02-12 | 2005-08-25 | Eisai Co., Ltd | Quinone remedies for lung diseases |
| JP5103608B2 (en) * | 2006-02-10 | 2012-12-19 | 国立大学法人名古屋大学 | Pharmaceutical composition for treatment or prevention of liver cancer |
Family Cites Families (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JPS63185921A (en) * | 1987-01-28 | 1988-08-01 | Takeda Chem Ind Ltd | Remedy for hepatic disease |
-
2003
- 2003-06-12 CA CA002488880A patent/CA2488880A1/en not_active Abandoned
- 2003-06-12 BR BR0311781-2A patent/BR0311781A/en not_active IP Right Cessation
- 2003-06-12 AU AU2003277184A patent/AU2003277184B2/en not_active Ceased
- 2003-06-12 WO PCT/JP2003/007491 patent/WO2003105819A1/en active Application Filing
Also Published As
| Publication number | Publication date |
|---|---|
| BR0311781A (en) | 2005-04-12 |
| WO2003105819A1 (en) | 2003-12-24 |
| AU2003277184B2 (en) | 2007-11-01 |
| AU2003277184A1 (en) | 2003-12-31 |
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