CA2462873A1 - Flea head, nerve cord, hindgut and malpighian tubule nucleic acid molecules, proteins and uses thereof - Google Patents

Abstract

The present invention relates to flea head, nerve cord, hindgut and Malpighian tubule proteins; to flea head, nerve cord, hindgut and Malpighian tubule nucleic acid molecules, including those that encode such flea head, nerve cord, hindgut and Malpighian tubule proteins; to antibodies raised against such flea head, nerve cord, hindgut and Malpighian tubule proteins; and to compounds that inhibit flea head, nerve cord, hindgut and Malpighian tubule protein activity. The present invention also includes methods to obtain such proteins, nucleic acid molecules, antibodies, and inhibitory compounds. Also included in the present invention are therapeutic compositions comprising proteins, nucleic acid molecules, antibodies, or protective compounds derived from proteins of the present invention as well as the use of such therapeutic compositions to protect animals from flea infestation. Also included in the present invention is the use of flea head, nerve cord, hindgut and Malpighian tubule proteins to derive inhibitory compounds.

Description

FLEA HEAD, NERVE CORD, HINDGUT AND MALPIGHIAN TUBULE
NUCLEIC ACID MOLECULES, PROTEINS AND USES THEREOF
FIELD OF THE INVENTION
The present invention relates to nucleic acid molecules isolated from the head and nerve cord of a flea, nucleic acid molecules isolated from the hindgut and Malpighian tubule of a flea, proteins encoded by such nucleic acid molecules, antibodies raised against such proteins, and inhibitors of such proteins. The present invention also includes therapeutic compositions comprising such nucleic acid molecules, proteins, antibodies, and/or other inhibitors, as well as uses thereof.
BACKGROUND OF THE INVENTION
Flea infestation of animals is a health and economic concern to pet owners. In particular, the bites of fleas are a problem for animals maintained as pets because the infestation becomes a source of annoyance not only for the pet but also for the pet owner who may find his or her home generally contaminated with insects. Fleas are known to directly cause a variety of diseases, including allergies, and also carry a variety of infectious agents including, but not limited to, endoparasites (e.g., nematodes, cestodes, trematodes and protozoa), bacteria and viruses. As such, fleas are a problem not only when they are on an animal but also when they are in the general environment of the animal.
Bites from fleas are a particular problem on many animals because they can cause a hypersensitive response in animals which is manifested as disease. For example, bites from fleas can cause an allergic disease called flea allergic (or allergy) dermatitis (FAD). A hypersensitive response in animals typically results in localized tissue inflammation and damage, causing substantial discomfort to the animal.
The medical importance of flea infestation has prompted the development of reagents capable of controlling flea infestation. Commonly encountered methods to control flea infestation are generally focused on use of insecticides, which are often unsuccessful for one or more of the following reasons: (1) failure of owner compliance (frequent administration is required); (2) behavioral or physiological intolerance of the pet to the pesticide product or means of administration; and (3) the emergence of flea populations resistant to the prescribed dose of pesticide.

-2-Thus, there remains a need to develop a reagent and a method to protect animals from flea infestation.
SUMMARY OF THE INVENTION
The present invention relates to a novel product and process for protection of animals from flea infestation.
The present invention provides flea head and nerve cord (HNC) proteins and flea hindgut and Malpighian tubule (HMT) proteins; nucleic acid molecules encoding flea HNC proteins and flea HMT proteins; antibodies raised against such proteins (i.e., anti-flea HNC antibodies and anti-flea HMT antibodies respectively); mimetopes of such proteins or antibodies; and compounds that inhibit flea HNC or HMT
activity (i.e, inhibitory compounds or inhibitors).
The present invention also includes methods to obtain such proteins, mimetopes, nucleic acid molecules, antibodies and inhibitory compounds. The present invention also includes the use of proteins and antibodies to identify such inhibitory compounds as well as assay kits to identify such inhibitory compounds. Also included in the present invention are therapeutic compositions comprising proteins, mimetopes, nucleic acid molecules, antibodies and inhibitory compounds of the present invention including protective compounds derived from a protein of the present invention that inhibit the activity of HNC and/or HMT proteins; also included are uses of such therapeutic compounds to reduce flea infestation.
One embodiment of the present invention is an isolated nucleic acid molecule that hybridizes with a nucleic acid molecule selected from the group consisting of a nucleic acid sequence of Table T and/or Table II, or a nucleic acid sequence complementary to a nucleic acid sequence of Table I and/or Table II under conditions that allow less than or equal to 30% base pair mismatch.
Another embodiment of the present invention is an isolated nucleic acid molecule having a nucleic acid sequence that is at least 70% identical to a nucleic acid sequence of Table I and/or Table II or complements thereof as well as fragments of such sequences.
The present invention also relates to recombinant molecules, recombinant viruses and recombinant cells that include a nucleic acid molecule of the present

-3-invention. Also included are methods to produce such nucleic acid molecules, recombinant molecules, recombinant viruses and recombinant cells.
Another embodiment of the present invention includes an isolated flea HMT
and/or HNC protein that is at least 70% identical to an amino acid sequence encoded by a nucleic acid sequence of Table I and/or Table II, and fragments thereof, wherein such fragments can elicit an immune response against respective flea proteins or have activity comparable to respective flea proteins.
Another embodiment of the present invention includes an isolated protein encoded by a nucleic acid molecule that hybridizes with the complement of a nucleic acid sequence of Table I and/or Table II, under conditions that allow less than or equal to 30% base pair mismatch.
DETAILED DESCRIPTION OF THE INVENTION
The present invention provides for nucleic acid molecules isolated from the head and/or nerve cord of a flea, nucleic acid molecules isolated from the hindgut and/or Malpighian tubule of a flea, proteins encoded by such nucleic acid molecules, antibodies raised against such proteins, and inhibitors of such proteins. As used herein, nucleic acid molecules isolated from the head and/or nerve cord of a flea and proteins encoded by such nucleic acid molecules are also referred to as flea HNC, or HNC, nucleic acid molecules and proteins respectively; and nucleic molecules isolated from the hindgut and/or Malpighian tubules of a flea and proteins encoded by such nucleic acid molecules are referred to as flea HMT or HMT, nucleic acid molecules and proteins respectively. HNC nucleic acid molecules and HMT nucleic acid molecules of the present invention are nucleic acid molecules that are primarily expressed in flea HNC tissues and HMT tissues respectively, but which may be expressed in cells derived from flea tissues other than HNC and HMT. HNC and HMT nucleic acid molecules and proteins of the present invention can be isolated from a flea or prepared recombinantly or synthetically. HMT and HNC nucleic acid molecules of the present invention can be RNA or DNA; examples of nucleic acid molecules include, but are not limited to, complementary DNA (cDNA) molecules, genomic DNA molecules, synthetic DNA molecules, DNA molecules which are specific tags for messenger RNA derived from HMT and HNC tissues, and

-4-corresponding mRNA molecules. As such, a flea nucleic acid molecule of the present invention is not intended refer to an entire chromosome within which such a nucleic acid molecule is contained, however, a flea HMT or HNC cDNA of the present invention may include all regions such as regulatory regions that control production of flea peritrophin proteins encoded by such a cDNA (such as, but not limited to, transcription, translation or post-translation control regions) as well as the coding region itself, and any introns or non-translated coding regions. As used herein, the phrases "HMT and/or HNC protein" and "HMT and HNC protein" refer to a protein expressed by a flea HMT tissue, by a flea HNC tissue, or by both flea HMT and HNC
tissues. As used herein, the phrases "HMT and/or HNC nucleic acid molecule"
and "HMT and HNC nucleic acid molecule" refer to a nucleic acid molecule that can be isolated from a HMT cDNA library, from a HNC cDNA library, or from both libraries, or a gene corresponding thereto.
The present invention also provides for HMT and HNC DNA molecules that are specific tags for messenger RNA molecules derived from HMT and HNC
tissues.
Such DNA molecules can correspond to an entire or partial sequence of a messenger RNA, and therefore, a DNA molecule corresponding to such a messenger RNA
molecule (i.e. a cDNA molecule), can encode a full-length or partial-length protein. A
nucleic acid molecule encoding a partial-length protein can be used directly as a probe or indirectly to generate primers to identify and/or isolate a cDNA nucleic acid molecule encoding a corresponding, or structurally related, full-length protein. Such a partial cDNA nucleic acid molecule can also be used in a similar manner to identify a genomic nucleic acid molecule, such as a nucleic acid molecule that contains the complete gene including regulatory regions, exons and introns. Methods for using partial HMT and HNC cDNA molecules and sequences.to isolate full-length transcripts and corresponding cDNA molecules are described in the examples herein below.
The proteins and nucleic acid molecules of the present invention can be obtained from their natural source, or can be produced using, for example, recombinant nucleic acid technology or chemical synthesis. Also included in the present invention is the use of these proteins and nucleic acid molecules as well as

-5-antibodies and inhibitory compounds thereto as therapeutic compositions to protect animals from flea infestation as well as in other applications, such as those disclosed below.
Flea HMT and HNC proteins and nucleic acid molecules of the present invention have utility because they represent novel targets for anti-arthropod vaccines and chemotherapeutic drugs. The products and processes of the present invention are advantageous because they enable the inhibition of arthropod development, metamorphosis, feeding, digestion and/or reproduction processes that involve HMT
and/or HNC proteins.
The head and nerve cord of the flea, including antennae, brain, corpora cardiacum, corpora allata, and subesophageal and abdominal ganglion tissues are of interest as such tissues are highly enriched for transcripts that encode neuronal and endocrine targets, as well as targets involved in chemosensory and mechanosensory reception. By sequencing cDNA fragments from a library enriched in flea head and nerve cord nucleic acid sequences (referred to herein as HNC nucleic acid sequences), genes, and their respective full-length coding regions, integrally involved with flea neuronal and endocrine function are identified. Once identified, these genes can be further characterized and specific interference strategies are designed. As such, flea HNC proteins and nucleic acid molecules of the present invention have utility because they represent novel targets for anti-arthropod vaccines and chemotherapeutic drugs.
Blood-feeding insects such as fleas ingest large quantities of blood relative to their body weight and, as such, are adapted to reduce the volume of the ingested blood meal through the rapid elimination of water. In addition, the concentrations of sodium, potassium, and chloride ions in the blood meal are greater than in the hemolymph of fleas, necessitating the excretion of excessive amounts of these ions. The active transport of these ions from the hemolymph into the lumens of the Malpighian tubules and the hindgut drives the passive transport of water and other hemolymph contents into these organs as well. While passing through these organs, waste products from the hemolymph are excreted and needed nutrients, water, and salts are reabsorbed. As such, interfering with these essential processes is an important strategy for developing a product for controlling flea populations. By sequencing cDNA fragments from a

-6-library enriched in hindgut and Malpighian tubule nucleic acid sequences (referred to herein as HMT nucleic acid sequences), genes integrally involved with these processes, and their respective full-length coding regions, are identified.
Once identified, these genes are further characterized and specific interference strategies can be designed. As such, flea HMT proteins and nucleic acid molecules of the present invention have utility because they represent novel targets for anti-arthropod vaccines and chemotherapeutic drugs.
One embodiment of the present invention is an isolated protein that includes a flea HMT and/or HNC protein. It is to be noted that the term "a" or "an"
entity refers to one or more of that entity; for example, a protein, a nucleic acid molecule, an antibody and a therapeutic composition refers to "one or more" or "at least one"
protein, nucleic acid molecule, antibody and therapeutic composition respectively. As such, the terms "a" (or "an"), "one or more" and "at least one" can be used interchangeably herein. It is also to be noted that the terms "comprising", "including", and "having" can be used interchangeably. According to the present invention, an isolated, or biologically pure, protein, is a protein that has been removed from its natural milieu. As such, "isolated" and "biologically pure" do not necessarily reflect the extent to which the protein has been purified. An isolated protein of the present invention can be obtained from its natural source, can be produced using recombinant DNA technology, or can be produced by chemical synthesis.
As used herein, isolated flea HMT and/or HNC proteins of the present invention can be full-length proteins or any homologue of such proteins. An isolated protein of the present invention, including a homologue, can be identified in a straight-forward manner by the protein's ability to elicit an immune response against a flea HMT and/or HNC protein or by the protein's HMT and/or HNC activity. Examples of flea HMT and HNC homologue proteins include flea HMT and HNC proteins in which amino acids have been deleted (e.g., a truncated version of the protein, such as a peptide), inserted, inverted, substituted and/or derivatized (e.g., by glycosylation, phosphorylation, acetylation, myristoylation, prenylation, palmitoylation, amidation andlor addition of glycerophosphatidyl inositol) such that the homologue includes at least one epitope capable of eliciting an immune response against a flea HMT
or HNC

7 PCT/US02/31878 protein, and/or of binding to an antibody directed against a flea HMT or HNC
protein.
That is, when the homologue is administered to an animal as an immunogen, using techniques known to those skilled in the art, the animal will produce an immune response against at least one epitope of a natural flea HMT or HNC protein.
The ability of a protein to effect an immune response can be measured using techniques known to those skilled in the art. As used herein, the term "epitope" refers to the smallest portion of a protein or other antigen capable of selectively binding to the antigen binding site of an antibody or a T cell receptor. It is well accepted by those skilled in the art that the minimal size of a protein epitope is about four to six amino acids. As is appreciated by those skilled in the art, an epitope can include amino acids that naturally are contiguous to each other as well as amino acids that, due to the tertiary structure of the natural protein, are in sufficiently close proximity to form an epitope. According to the present invention, an epitope includes a portion of a protein comprising at least 4 amino acids, at least 5 amino acids, at least 6 amino acids, at least 10 amino acids, at least 15 amino acids, at least 20 amino acids, at least 25 amino acids, at least 30 amino acids, at least 35 amino acids, at least 40 amino acids or at least 50 amino acids in length.
In one embodiment of the present invention a flea homologue protein has HMT
or HNC activity, i.e. the homologue exhibits an activity similar to its natural counterpart. Methods to detect and measure such activities are known to those skilled in the art.
Flea HMT and/or HNC homologue proteins can be the result of natural allelic variation or natural mutation. Flea HMT andlor HNC protein homologues of the present invention can also be produced using techniques known in the art including, but not limited to, direct modifications to the protein or modifications to the gene encoding the protein using, for example, classic or recombinant DNA techniques to effect random or targeted mutagenesis.
Flea HMT and HNC proteins of the present invention are encoded by flea HMT and HNC nucleic acid molecules, respectively. As used herein, flea HMT and HNC nucleic acid molecules include nucleic acid sequences related to natural flea HMT and HNC genes, and, preferably, to Cteraocephalides felis HMT and HNC
genes.

_g_ As used herein, flea HMT and HNC genes include all regions such as regulatory regions that control production of flea HMT and HNC proteins encoded by such genes (such as, but not limited to, transcription, translation or post-translation control regions) as well as the coding region itself, and any introns or non-translated coding regions. As used herein, a nucleic acid molecule that "includes" or "comprises" a sequence may include that sequence in one contiguous array, or may include the sequence as fragmented exons such as is often found for a flea gene. As used herein, the term "coding region" refers to a continuous linear array of nucleotides that translates into a protein. A full-length coding region is that coding region that is translated into a full-length, i.e., a complete protein as would be initially translated in its natural millieu, prior to any post-translational modifications.
Table I represents a variety of flea HNC nucleic acid molecules of the present invention.
SE ~D NO Name;
' HNC

8 2229-44HNC

9 2249-09HNC

10 2249-lOHNC

11 2251-09HNC

12 2215-13HNC

13 2218-39HNC

14 2218-82HNC

Table II represents a variety of flea HMT nucleic acid molecules of the present invention.
SE m NO: Name 20 2084-49HMT
'

15 2084-03HMT 21 2084-51 HMT

16 2084-27HMT 22 2085-1OHMT

17 2084-41HMT 23 2085-23HMT

18 2084-45HMT 24 2085-25HMT

19 2084-47HMT 25 2085-41HMT

_9_ SE ID NO: Name HMT

HMT

61 2108-~22HMT 104 2191-66HMT

SE m NO: Name 152 2089-lOHMT 195 2255-45HMT

SE ID Name NO

207 2256-~64HMT 250 2241-O8HMT

225 2233-~39HMT 268 2230-70HMT

-MT

SE ID NO: Name 293 2230-60HMT 327 2243-lOHMT

315 2241-lOHMT 349 2244-71HMT

Table III represents highest Blast hits for nucleic acid molecules of the present invention.
TD Descri tion ' - S ecies No_ 3084 3 Actin, cyto lasmic Boznbyx mori 3084 27 ribosomal rotein 112 Rattus norvegicus 3084 41 Elon anon factor 2 (EF-2) Droso laila melano aster 3084 45 60S ribosomal rotein L5 Bonzb x zzzori ~, Descri tion S ecies No.

3084 47 Acidic ribosomal rotein r Droso hila znelazzo A aster 3084 49 CG5827 ene rodu Droso lzila nzelano aster 3084 51 Ribosomal rotein s7 Manduca sexta 3085 10 Am hi h sin Droso hila melano aster 3085 23 CG10315 ene roduct Droso hila nzelano aster 3085 41 R S4 ene roduct Droso lzila nzelano aster 3085 57 CG3380 ene roduct Droso lzila melazzo aster 3086 22 Cofactor C Homo sa iens 3087 18 unknown Drosoplzila melanogaster 3088 24 cell adhesion molecule a CAM California sea hare (clone d12) 3088 27 elon ation factor 1 al ha Mayetiola destructor 3088 30 CG12775 ene roduct Droso hila melaszo aster 3088 51 cram osin - nematode Onchocerca volvulus 3088 53 succinate deh dro enase flavoDroso hila melano rotein subunit aster-3089 52 CG13806 ene roduct Droso hila nzelano aster 3091 32 Ac 1 carrier rotein, mitochondria)Droso hila melafzo recursor aster 3091 62 Protein disulfide isomerase Droso hila nzelano recursor aster 3092 34 siah bindin rotein Honzo sa iens 3094 14 aconitase Da hnia ulex 3094 18 excision re air rotein ERCC21XPDDroso lzila nzelanogaster 3094 46 CG7176 ene roduct Droso lzila nzelano aster 3094 61 elon ation factor-1 al ha Cy honia clavata 3095 14 kettin Droso hila nzelanogaster 3095 25 CG7791 ene roduct Drosoplzila melano aster 3095 50 CG3861 ene roduct Droso hila melano aster 3102 6 CG4288 ene roduct [alt 1] Drosoplzila melano aster 3102 25 Pol -A bindin rotein, Chain Xeno us laevis E

3102 26 Elon ation factor 2 Drosophila nzelanogaster 3103 15 human of aden late bindin Homo sapiezzs rotein II

3104 14 CG9244 ene roduct [alt 1] Droso hila melano aster 3104 16 translation elon anon factor Droso hila melazzo 2 aster 3105 4 ribos ma) rotein s5 Mus musculus 3105 7 vanin-like rotein Drosoplzila zrzelanogaster 3105 25 NADH deh dro enase Homo sapiens 3105 41 CG5214 ene roduct Droso hila melano aster 3107 21 ribosomal rotein L8 Azzo heles ambiae 3107 36 ribosomal rotein Ll8a Honzo sa iens 3108 8 vacuolar ATPase SFD subunit Drosophila melano aster 3108 12 CG6439 ene roduct Drosophila melano aster 3108 22 CG8415 ene roduct Droso hila nzelanogastez-3124 3 TSC2 Takifu a rubri es 3154 17 CG11771 ene roduct Drosoplzila melazzo aster 3154 18 elon ation factor 1 al ha Acam sis alterni es 3154 49 CG11266 ene roduct Droso hila nzelanogaster 3154 52 elon ation factor 2 Droso hila melazzo aster 3154 55 NADPH ferrihemo rotein reductaseDroso lzila melano aster 3154 57 CG7144 ene roduct Droso hila melazzo aster 3156 5 CG8201 ene roduct [alt 1] Droso hila nzelano aster 3156 57 mini aram osin Droso hila rnelano astez-3157 40 fimbrin Droso hila melano aster ID Description S ecies No.

3158 8 mitochndrial hos hate carrierDz-oso Izila melano rotein aster 3159 78 nuclear rotein SA Droso hila nzelano aster 3160 38 ribosomal rotein S17 Droso hila melazzo aster 3160 87 CG3872 ene roduc Dt'OSO hila nzelanogaster 3162 16 fimbrin Droso l2ila tnelano aster 3162 20 HL23 ribosomal rotein Hozzzo sa iezts 3162 74 NADH-ubi uinone oxidoreductaseBos taurus 3166 25 d 115 rotein Droso lzila melano aster 3167 46 ribosomal rotein S24 Fu a rubri es 3169 34 Proline s nthetase associatedHonzo sa iens 3171 31 translocation rotein 1 Droso hila nzelanogaster 3171 81 CG4600 ene roduct Droso hila nzelano aster 3173 28 CG15077 ene roduct Droso hila inelano aster 3173 61 ADP/ATP translocase Oryctolagus cuniculus 3175 12 CG5037 ene roduct Droso hila yakuba 3175 26 ribosomal rotein SS A is mellifera 3175 77 c tochrome b Droso Izila melatzo aster 3177 26 c tochrome oxidase II Cteztoee halides,felis 3177 42 CG8728 ene roduct Drosophila melanogaster 3177 50 ruvate deh dro enase t a 1 Caenorhabditis ale al ha subunit ans 3177 82 translation re ressor NATl Gallus allus 3181 65 ubi uinol-c tochrome c reductaseHomo sa iens 3181 85 h othetical rotein FL,J20417 Hotno sa iens 3183 34 Heat shock rotein 70 Ceratitis ca itata 3183 77 ADP ribos lation factor 2 Drosophila nzelano aster 3183 90 lutamate dehydro enase Droso hila melano astez~

3187 45 ribosomal rotein S4 Drosophila melazzo aster 3188 28 RAS-related rotein RAB-5C Mus nzusculus 3188 75 CG7144 ene roduct D7"OSO hila m.elartogaster 3191 66 ATP-specific succinyl-CoA Mus musculus synthetase beta subunit 3192 23 D-Titin Drosophila melan aster 3192 42 ro'ectin Dz-oso hila nzelangaster 3193 50 CG15221 ene roduct Droso hila melazzo aster 3194 30 K7 kinesin-like rotein Dictyostelium discoideum 3194 37 CG9498 ene roduct Droso hila melazzo aster 3195 34 CG10320 ene roduct Droso Izila zzzelano aster 3195 80 h othetical rotein C56E6.6 C. ale ans 3196 4 ut. ribosomal rotein L1 Drosophila melanogaster 3196 29 eukaryotic translation initiationDrosopihila melanogaster factor 4A
(e1F-4A) 3197 24 0l aden late bindin rotein Petronzyzon marinus 3202 47 eukar otic translation initiationMus nzusculus factor 4A

3205 78 c toskeletal actin Styela clava 3253 2 c tochrome C oxidase H. sa iens 3253 73 CG6090 ene roduct Droso hila melano aster 3253 83 translation elon anon factor Droso hila melano 2 aster 3253 84 NADH dehydro enase subunit Azzo hales gambiae 3254 23 elon ation factor 1- aroma Artemia 3254 40 cDNA EST k96f8.5 C. ale azzs ID Descri tion S ecies No.
.

3254 57 h othetical rotein SC3C3.03c Stre tonz ces oelicolorA3(2) 3254 87 CG5891 ene roduct Droso hila rnelano aster 3255 61 0l h drox allcanoic s nthase,Alcali eves eutro PHA s nthase hus 3255 80 CG3648 ene roduct Droso hila melano aster 3256 5 CG5827 ene roduct Droso hila melano aster 3086 20 mini-colla en Hydra 3086 53 60S ribosomal rotein L4 Droso hila melanogaster 3087 6 beta-tubulin Droso hila melano aster 3088 5 Rab 5c rotein Carzis familiar-is 3088 17 beta subunit RNA of merase Plasmodium, falci arum 3089 10 beta ada tin Droso hila melano aster 3094 33 h othetical rotein CO1B 12.1 C. ele ans 3094 47 utative lutathione s nthetaseMedica o truncatula 3103 28 CG10602 ene roduct Droso hila melano aster 3104 23 CG4164 ene roduct Droso lzila nzelano aster 3104 59 CG6529 ene roduct Droso hila melanogaster 3105 9 CG4090 Droso hila melanogaster 3106 14 kinesin-like rotein Droso hila nzelano aster 3156 3 clathrin-associated adaptor Drosoplzila melanogaster complex AP-1 medium chain 3156 14 CG11963 ene roduct Droso hila melano aster 3162 93 CG11267 ene roduct Droso hila melano aster-3175 18 Probable E4 rotein Human a illomavirus ty a 6a 3185 19 c tochrome oxidase II Ctenoce halides, felis 3191 92 CG13655 ene roduct Droso hila melano asrer 3196 61 c to lasmic elsolin Droso ihila nzelano aster 3253 13 translation initiation factorCG18445 erze roduct 3253 16 m osin 1i ht chain 2 Droso hila melano aster 3253 19 CG14206 ene roduct Droso hila melanogaster 3253 27 larval and adult m osin heav Droso' lzila melano chain aster 3253 40 Hetero eneous nuclear ribonucleoDrosophila melanogaster rotein 27C

3253 56 rofilin Droso l2ila melano aster 3253 68 NADH-ubiquinone oxidoreductaseDrosophila melanogaster 42 kDa subunit 3253 78 CG7903 ene roduct Droso hila nzelanogaster 3253 87 ferritin subunit 1 Droso hila melano aster 3253 92 m osin 1i ht chain 2 Droso hila melanogaster 3254 43 muscle m osin heav chain Droso hila melarzo aster 3254 45 CG3714 ene roduct Droso Izila melano aster 3254 51 60S ribosomal rotein Mus rrzusculus 3254 54 CG10753 ene roduct Droso hila nzelano aster 3254 66 poly-U-binding-splicing-factorDrosoplzila rnelarzogaster gene product [alt 1]

3254 88 al ha-s ectrin Droso hila melano aster 3254 96 CG2852 ene roduct Droso hila melano aster 3255 17 ribonucleoside reductase M1 Droso hila melano subunit aster 3255 19 dockin rotein Honzo sa iens 3255 23 ribosomal rotein S31 Droso hila nzelano aster 3255 25 isovale 1 deh dro enase recursorMus musculus 3255 32 ribosomal protein L7a Droso hila melano aster ID o. Descri tion S ecies N

3255 39 CG10627 ene roduct Droso bile nzelano aster 3255 41 NADH deb dro enase subunit Droso bile mauritiana 3255 45 BcDNA:GH11673 ene roduct Droso bile melazzo aster 3255 60 Sec23 rotein Homo sa iens 3255 62 HMG CoA s nthase M. znusculus 3255 67 CG7920 ene roduct Droso bile znelano aster 3255 77 CG14206 ene rodu Droso lzila znelano aster 3255 79 40S ribosomal rotein SA Droso bile ntelanogastez-3255 82 RNA-bindin rotein lark Droso bile melano aster 3256 12 CG7821 ene roduct Droso bile znelazto aster 3256 22 Eukar otic translation initiationDroso bile melano factor 6 astez-3256 46 h drox roline-rich rotein Volvox carteri.f.
zta ariezzsis 3256 54 PDGF associated rotein Homo sa iezzs 3256 58 S20 ribosomal rotein Droso bile zzzelazzo aster 3256 64 ribosomal rotein S9 Droso bile znelazto aster 3229 13 myosin-IB Droso bile nzelazto aster 3229 17 NADH deb dro enase subunit Bontb x mori 3229 20 c tochrome b Beamys hindei 3229 31 CG1967 ene roduct Droso bile melan.o aster 3229 33 r rotein Drosophila zzzelazzo aster 3229 34 ferritin 2 li ht chain homoloDroso bile melano aster 3232 63 NADH deb dro enase subunit Azzo heles ambiae 3232 70 ubi uitin-h brid rotein recursorDroso bile melano aster 3232 81 ribosomal rotein L23a Droso h.ila m.elano aster 3232 96 ribosomal rotein RSP14A Droso bile znelazto aster 3233 3 malate deb dro enase Mus nzusculus 3233 4 cal onin-like rotein Chd64 Droso bile melano aster 3233 9 aram osin Droso laila nzelano aster 3233 14 CG2099 ene roduct Droso bile melano aster 3233 20 CG11963 ene rodu Droso Izila nzelano astez 3233 33 succinyl-CoA:alpha-ketoacid Sus scrofa coenzyme A
transferase 3233 38 unknown Homo Sapiens 3233 39 CG12736 ene roduct Droso bile melanogaster 3233 48 R L11 ene roduct Droso bile nzelano aster 3233 54 minute(1)1B rotein Droso bile nzelano astez-3233 56 ribosomal rotein L9 Drosophila melano aster 3233 60 CG10124 ene roduct Droso bile melano aster 3233 64 rotein 4.1 homolo a (coracle)Droso bile zzzelanogaster 3233 70 mitochondria) ND5 gene for Piezis rapae NADH
deb dro enase subunit 5 3233 71 CG9515 ene roduct Drosophila melano aster 3233 75 utative runasin h drolase Prunus serotina recursor 3233 77 ribosomal rotein 15a Droso bile melano aster 3233 92 ribosomal rotein S 18 Droso bile melano aster 3233 95 CG3006 ene roduct Droso bile melazto aster 3234 8 beta-5 tubulin Gallus allus 3234 15 h drox roline-rich rotein Volvox carteri, f.
na ariensis 3234 17 m osin-IB Droso bile znelanogaster 3234 18 muscle myosin heavy chain ~Drosophila melano~aster ID Descri tion S ecies No.

3234 30 ribosomal rotein L31 Aedes ae y ti 3234 33 c tochrome P450 Musca domestica 3234 35 carnitine almito ltransferaseDz-oso lz.ila melanogastez-I

3234 37 non-muscle m osin Droso hila melano aster 3234 57 Aldeh de-dehydro enase lilce C. ele ans rotein 3234 65 ribosomal rotein S6 Manduca sexta 3240 4 fucosidase, al ha-L-1, tissueRattus nor-ve icus 3241 7 Irate in beta-subunit m os Droso hila melano heroid recursor astez-3229 71 aldeh de deh dro enase Placopecten znagellanicus 3229 86 lutamine s nthetase Ano Iz.eles ambiae 3230 4 c tochrome C oxidase III Ceratitis ca itata 3230 9 TRIP-1 homolo Droso laila m.elano aster 3230 12 sta ene roduct Drosophila melazzo aster 3230 13 BcDNA.GM04779 Drosophila melano aster 3230 30 BcDNA.GM12291 Droso hila nzelano aster 3230 31 hnRNP rotein Chironomus tesztazzs 3230 36 SURF-4 rotein Droso hila melazzo aster 3230 40 similar to ubi uitin family C. ele ans 3230 42 ribosomal rotein L19 Droso hila znelano aster 3230 44 CG5651 ene roduct Droso hila zzzelano aster 3230 45 CG4046 ene roduct Droso hila nzelano aster 3230 61 CG1475 ene rodu Droso hila melano aster 3230 63 CG17489 ene roduct Droso h.ila melano aster 3230 68 40S ribosomal rotein S 12 Droso hila melano aster 3230 70 CG2062 ene roduct Droso hila melano aster 3230 72 ribosomal rotein L41 Mus musculus 3230 76 CG1837 ene roduct Droso hila melazzogaster 3230 78 lost ene roduct Dz-osoplzila melano aster 3230 86 ribosomal rotein L8 Aedes albopictus 3230 92 CG12082 ene roduct Droso hila nzelano aster 3231 5 CG10423 ene roduct Droso lzila melano aster 3231 6 CG7424 ene roduct Droso Izila nzelano aster 3231 9 1 ceraldeh de-3- hos hate Droso hila melanogaster deh dro enase-1 3231 12 c to lasmic actin Pisaster ochraceus 3231 17 CG1628 ene roduct Drosoplzila melano aster 3231 30 BcDNA.GH06451 Droso hila melazzo aster 3231 34 ribosomal rotein 49 Droso hila melano aster 3231 45 myosin heav -chain a Drosophila melano aster 3231 96 moesin/ezrin/radixin homolo Drosophila nzelano aster 3232 7 calreticulin Droso hila melanogaster 3232 9 ribosomal rotein S 19 Drosophila melanogaster 3232 13 tro omyosin Peri lazzeta aznericana 3232 23 CG13806 ene roduct Drosophila melanogaster 3232 31 h othetical rotein FLJ20604 Hozno sa iens 3232 35 hemomucin Drosophila melazzogaster 3232 40 CG6287 ene roduct Droso laila melano aster 3232 46 mitochondria) cytochrome oxidaseA rius cozzvolvuli I

3232 54 tro om osin I isoform a Droso hila melanogaster 3229 51 isocitrate deh dro enase Homo sa iezzs 3230 60 CG7662 gene product ~ Drosophila nzelanogaster ID. o. Descri tion S ecies N

3230 81 DISCS LOST; dlt DI'OSO hila nzelano aster 3231 3 CG8857 ere roduct Droso lzila melazzo aster 3231 24 97 subunit of 15S M (2+)- Xeno us laevis ATPase 3231 56 c tochrome b Phlebotonzus bar eroti 3231 59 CG6180 ere roduct Droso lzila melano aster 3234 12 secretor mucin Honzo sa iezzs 3234 76 CG2674 ere roduct [alt 1] Droso hila znelano aster 3234 86 ribosomal rotein L3 Bombyx mori 3240 11 CG7404 ere roduct Droso hila nzelano aster 3240 14 CG9140 ere roduct Droso hila melano aster 3240 17 CG7973 ere roduct Droso hila melanogaster 3240 23 s m lekin Homo sa iens 3240 26 CG9282 ere roduct Droso lzila melanogaster 3240 28 CG7283 ere roduct Droso hila zzzelano astez-3240 31 eukar otic initiation factor Homo sa iens 4 aroma 3240 44 unknown Dz~oso lzila melano aster 3240 63 ferritin subunit 1 Droso hila melanogaster 3240 66 utative t-com lax of a tide Aedes tziseriatus 1 delta 3240 70 CG4729 ere roduct [alt 2] Droso lzila melano aster 3240 72 CG9332 ere roduct Drosophila nzelano aster 3240 94 1 carol-3- hos hate deh dro Ceratitis ca itata erase 3241 10 Sam50 Droso hila melano aster 3241 12 NADH deh dro erase subunit Bonzbyx znori 3241 40 EF-1-al ha Droso hila zzzelanogaster 3241 54 CG2998 ere roduct Droso hila nzelano aster 3241 56 short-chain alcohol deh dro Honzo sa iens erase 3241 60 muscle t a hos hofructokinaseDroso Izila melano aster 3241 66 Dolichyl-diphosphooligosaccharide--proteinDrosophila nzelazzogaster 1 cos ltransferase 48 kd subunit 3241 74 enolase Hymenole is dizzzizzuta 3241 82 aramyosin Droso hila nzelano aster 3241 83 beta-tubulin Bonzbyx mori 3241 86 BcDNA:GH04637 ere roduct [altDz-oso hila nzelazzogastez-1]

3241 87 renin Rattus norvegicus 3243 10 al ha-actinin Droso hila nzelano aster 3243 18 acet 1-CoA s nthetase Droso laila melanogaster 3243 20 c tochrome P450 CYP12A3 Musca domestica 3243 22 NADH deh dro erase subunit Ano hales arabiensis 3243 27 olyubi uitin 5 Arabido sis thaliana 3243 28 moesin Drosophila nzelanogastez-3243 31 QM rotein Bombyx mazzdarina 3243 32 CG1250 ere roduct [alt 1] Droso Izila nzelatzogaster 3243 38 rojectin Droso hila nzelanogaster 3243 41 CG6543 ere roduct Droso hila nzelano aster 3243 45 CG13171 ere roduct Droso hila melanogaster 3243 48 neurofibromatosis type 2 proteinHozzzo Sapiens isoform Mer162 3243 52 utative succin 1-coa 1i ase Droso laila melano (GDP-formin ) aster 3243 73 CG7176 ere roduct [alt 3] Droso laila znelano aster 3243 86 Phos hoserine aminotransferaseHomo sa iens PSAT

ID Descri tion ,~ ecies No.

3_2_4_387 Bms d-2 Bonzbyx rnori 3243 94 CG3132 ene roduct Droso hila znelano aster 3244 12 tro om osin isoform 127 Droso hila znelano aster 3244 25 CG3132 ene roduct Droso hila rnelano aster 3244 29 NADH deh dro enase Bos taurus 3244 44 lutamate deh dro enase Droso hila melano aster 3244 54 s liceosomal rotein Droso hila melano aster 3244 71 eritro hin 1 Ano hales anzbiae 3244 75 NADH deh dro enase subunit Ano hales anzbiae 5 .

3227 82 CG7607 ene roduct Droso hila melano aster 3228 51 NADH deh dro enase subunit Cochliom is honzinivorax 3229 6 40S ribosomal rotein S 13 Drosophila melano aster 3229 42 Tubulin ALPHA-3/ALPHA-7 chainMus nausculus 3229 44 CG11916 ene roduct Droso hila nzelano aster 3249 9 heat shock rotein 60 Culicoides vaz-ii ennis 3249 10 tro onin T Peri laneta americana 3251 9 Citrate lyase alpha chain EscIZerichia coli ~

W one embodiment, a gene or other nucleic acid molecule of the present invention can be an allelic variant that includes a similar but not identical sequence to a C. felis nucleic acid sequence of Table I and/or Table II or a complement thereof.
For example, an allelic variant of a C. felis gene including SEQ ID NO:1 is a gene that occurs at essentially the same locus (or loci) in the genome as the gene including SEQ
ID NO: l, but which, due to natural variations caused by, for example, mutation or recombination, has a similar but not identical sequence. Because natural selection typically selects against alterations that affect function, allelic variants (i.e. alleles corresponding to, or of, cited nucleic acid sequences) usually encode proteins having similar activity to that of the protein encoded by the gene to which they are being compared. Allelic variants of genes or nucleic acid molecules can also comprise alterations in the 5' or 3' untranslated regions of the gene (e.g., in regulatory control regions), or can involve alternative splicing of a nascent transcript, thereby bringing alternative axons into juxtaposition. Allelic variants are well known to those skilled in the art and would be expected to occur naturally within a given flea such as C. felis, since the genome is diploid, and sexual reproduction will result in the reassortment of alleles.
In one embodiment of the present invention, isolated HMT and HNC proteins are encoded by nucleic acid molecules that hybridize under stringent hybridization

-20-conditions to genes or other nucleic acid molecules encoding flea HMT and HNC
proteins, respectively. The minimal size of HMT and HNC proteins of the present invention is a size sufficient to be encoded by a nucleic acid molecule capable of forming a stable hybrid (i.e., hybridizing under stringent hybridization conditions) with the complementary sequence of a nucleic acid molecule encoding the corresponding natural protein. The size of a nucleic acid molecule encoding such a protein is dependent on the nucleic acid composition and the percent homology between the flea HMT or HNC nucleic acid molecule and the complementary nucleic acid sequence.
It can easily be understood that the extent of homology required to form a stable hybrid under stringent conditions can vary depending on whether the homologous sequences are interspersed throughout a given nucleic acid molecule or are clustered (i.e., localized) in distinct regions on a given nucleic acid molecule.
The minimal size of a nucleic acid molecule capable of forming a stable hybrid with a gene encoding a flea HMT or HNC protein is typically at least about 12 to about 15 nucleotides in length if the nucleic acid molecule is GC-rich and at least about 15 to about 17 bases in length if it is AT-rich. The minimal size of a nucleic acid molecule used to encode an HMT or HNC protein homologue of the present invention is from about 12 to about 18 nucleotides in length. Thus, the minimal size of HMT or HNC
protein homologues of the present invention is from about 4 to about 6 amino acids in length. There is no limit, other than a practical limit, on the maximal size of a nucleic acid molecule encoding a flea HMT or HNC protein of the present invention because a nucleic acid molecule of the present invention can include a portion of a gene, an entire gene, or multiple genes. The preferred size of a protein encoded by a nucleic acid molecule of the present invention depends on whether a full-length, fusion, multivalent, or functional portion of such a protein is desired.
Stringent hybridization conditions are determined based on defined physical properties of the gene to which the nucleic acid molecule is being hybridized, and can be defined mathematically. Stringent hybridization conditions are those experimental parameters that allow an individual skilled in the a.rt to identify significant similarities between heterologous nucleic acid molecules. These conditions are well known to those skilled in the art. See, for example, Sambrook, et al., 1989, Molecular Cloning:

-21-A Laborato~ Manual, Cold Spring Harbor Labs Press, and Meinkoth, et al., 1984, Anal. Biochern. 138, 267-284, each of which is incorporated by reference herein in its entirety. As explained in detail in the cited references, the determination of hybridization conditions involves the manipulation of a set of variables including the ionic strength (M, in moles/liter), the hybridization temperature (°C), the concentration of nucleic acid helix destabilizing agents (such as formamide), the average length of the shortest hybrid duplex (n), and the percent G + C composition of the fragment to which an unknown nucleic acid molecule is being hybridized. For nucleic acid molecules of at least about 150 nucleotides, these variables are inserted into a standard mathematical formula to calculate the melting temperature, or Tm, of a given nucleic acid molecule. As defined in the formula below, Tm is the temperature at which two complementary nucleic acid molecule strands will disassociate, assuming 100%
complementarity between the two strands:
Tm 81.5°C + 16.6 log M + 0.41 (%G + C) - 500/n - 0.61 (%formamide).
For nucleic acid molecules smaller than about 50 nucleotides, hybrid stability is defined by the dissociation temperature (Td), which is defined as the temperature at which 50% of the duplexes dissociate. For these smaller molecules, the stability at a standard ionic strength is defined by the following equation:
Td=4(G+C)+2(A+T).
A temperature of 5°C below Td is used to detect hybridization between perfectly matched molecules.
Also well known to those skilled in the art is how base pair mismatch, i.e.
differences between two nucleic acid molecules being compared, including non-complementarity of bases at a given location, and gaps due to insertion or deletion of one or more bases at a given location on either of the nucleic acid molecules being compared, will affect Tm or Td for nucleic acid molecules of different sizes.
For example, Tm decreases about 1°C for each 1% of mismatched base pairs for hybrids greater than about 150 bp, and Td decreases about 5°C for each mismatched base pair for hybrids below about 50 bp. Conditions for hybrids between about 50 and about 150 base pairs can be determined empirically and without undue experimentation using standard laboratory procedures well known to those skilled in the art.
These

-22-simple procedures allow one skilled in the art to set the hybridization conditions (by altering, for example, the salt concentration, the helix destabilizing compound concentration or the temperature) so that only nucleic acid hybrids with greater than a specified % base pair mismatch will hybridize. Stringent hybridization conditions are commonly understood by those skilled in the art to be those experimental conditions that will allow less than or equal to about 30% base pair mismatch (i.e., at least about 70% identity). Because one skilled in the art can easily determine whether a given nucleic acid molecule to be tested is less than or greater than about 50 nucleotides, and can therefore choose the appropriate formula for determining hybridization conditions, he or she can determine whether the nucleic acid molecule will hybridize with a given gene under stringent hybridization conditions and similarly whether the nucleic acid molecule will hybridize under conditions designed to allow a desired amount of base pair mismatch.
Hybridization reactions are often carried out by attaching the nucleic acid molecule to be hybridized to a solid support such as a membrane, and then hybridizing with a labeled nucleic acid molecule, typically referred to as a probe, suspended in a hybridization solution. Examples of common hybridization reaction techniques include, but are not limited to, the well-known Southern and northern blotting procedures. Typically, the actual hybridization reaction is done under non-stringent conditions, i.e., at a lower temperature and/or a higher salt concentration, and then high stringency is achieved by washing the membrane in a solution with a higher temperature and/or lower salt concentration in order to achieve the desired stringency.
For example, if the skilled artisan wished to identify a nucleic acid molecule that hybridizes under conditions that would allow less than or equal to 30%
pair mismatch with a flea nucleic acid molecule of about 150 by in length or greater, the following conditions could preferably be used. The average G + C content of flea DNA is about 37%, as calculated from known flea nucleic acid sequences. The unknown nucleic acid molecules would be attached to a support membrane, and the 150 by probe would be labeled, e.g. with a radioactive tag. The hybridization reaction could be carried out in a solution comprising 2X SSC in the absence of helix destabilizing compounds, at a temperature of about 37°C (low stringency conditions).

-23-Solutions of differing concentrations of SSC can be made by one of skill in the art by diluting a stock solution of 20X SSC (175.3 gram NaCl and about 88.2 gram sodium citrate in 1 liter of water, pH 7) to obtain the desired concentration of SSC.
The skilled artisan would calculate the washing conditions required to allow up to 30%
base pair mismatch. For example, in a wash solution comprising 1X SSC in the absence of helix destabilizing compounds, the Tm of perfect hybrids would be about 77°C:
81.5°C + 16.6 log (.15M) + (0.41 x 0.37) - (500/150) - (0.61 x 0) =
77.5°C.
Thus, to achieve hybridization with nucleic acid molecules having about 30%
base pair mismatch, hybridization washes would be carried out at a temperature of less than or equal to 47.5°C. It is thus within the skill of one in the art to calculate additional hybridization temperatures based on the desired percentage base pair mismatch, formulae and G/C content disclosed herein. For example, it is appreciated by one skilled in the art that as the nucleic acid molecule to be tested for hybridization against nucleic acid molecules of the present invention having sequences specified herein becomes longer than 150 nucleotides, the Tm for a hybridization reaction allowing up to 30% base pair mismatch will not vary significantly from 47.5°C.
Furthermore, it is known in the art that there are commercially available computer programs for determining the degree of similarity between two nucleic acid or protein sequences. These computer programs include various known methods to determine the percentage identity and the number and length of gaps between hybrid nucleic acid molecules or proteins. Preferred methods to determine the percent identity among amino acid sequences and also among nucleic acid sequences include analysis using one or more of the commercially available computer programs designed to compare and analyze nucleic acid or amino acid sequences. These computer programs include, but are not limited to, the SeqLab~ Wisconsin PackageTM
Version 10.0-UNIX sequence analysis software (hereinafter referred to as "SeqLab"), available from Genetics Computer Group, Madison, WI; and DNAsisO sequence analysis software, version 2.0, available from Hitachi Software, San Bruno, CA. Such software programs represent a collection of algorithms paired with a graphical user interface for using the algorithms. The DNAsis version 2.0 software and SeqLab Wisconsin

-24-Package Version 10.0-UNIX software, for example, employ a particular algorithm, the Needleman-Wunsch algorithm to perform pair-wise comparisons between two sequences to yield a percentage identity score, see Needleman, S.B. and Wunch, C.D., 1970, J. Mol. Biol., 48, 443, which is incorporated herein by reference in its entirety.
Such algorithms, including the Needleman-Wunsch algorithm, are commonly used by those skilled in the nucleic acid and amino acid sequencing art to compare sequences.
A preferred method to determine percent identity among amino acid sequences and also among nucleic acid sequences includes using the Needleman-Wunsch algorithm, available in the SeqLab Wisconsin Package Version 10.0-UNIX software (hereinafter "SeqLab"), using the Pairwise Comparison/Gap function with the nwsgapdna.cmp scoring matrix, the gap creation penalty and the gap extension penalties set at default values, and the gap shift limits set at maximum (hereinafter referred to as "SeqLab default parameters"). An additional preferred method to determine percent identity among amino acid sequences and also among nucleic acid sequences includes using the Higgins-Sharp algorithm, available in the DNAsis version 2.0 software (hereinafter "DNAsis"), with the gap penalty set at 5, the number of top diagonals set at 5, the fixed gap penalty set at 10, the k-tuple set at 2, the window size set at 5, and the floating gap penalty set at 10. A particularly preferred method to determine percent identity among amino acid sequences and also among nucleic acid sequences includes using the Needleman-Wunsch algorithm available in the SeqLab software, using the SeqLab default parameters.
A preferred flea HMT and/or HNC protein includes a protein encoded by a nucleic acid molecule that hybridizes under conditions that preferably allow less than or equal to 30% base pair mismatch, preferably under conditions that allow less than or equal to 25% base pair mismatch, preferably under conditions that allow less than or equal to 20% base pair mismatch, preferably under conditions that allow less than or equal to 15% base pair mismatch, preferably under conditions that allow less than or equal to 10% base pair mismatch and preferably under conditions that allow less than or equal to 5% base pair mismatch with a nucleic acid molecule selected from the group consisting of a nucleic acid sequence complementary to a nucleic acid sequence of Table I andlor Table II.

-25-Another embodiment of the present invention includes a flea HMT and/or HNC protein encoded by a nucleic acid molecule that hybridizes under conditions comprising, (a) hybridizing in a solution comprising 1X SSC in the absence of helix destabilizing compounds, at a temperature of 37°C and (b) washing in a solution comprising 1X SSC in the absence of helix destabilizing compounds, at a temperature of 47.5°C, to an isolated nucleic acid molecule selected from the group consisting of a nucleic acid sequence complementary to a nucleic acid sequence of Table I
and/or Table II.
Another preferred flea HMT and/or HNC protein of the present invention includes a protein that is encoded by a nucleic acid molecule that is preferably at least 70% identical, preferably at least 75% identical, preferably at least 80%
identical, preferably at least 85% identical, preferably at least 90% identical, and preferably at least 95% identical to a nucleic acid molecule having a nucleic acid sequence of Table I and/or Table II; also preferred are fragments (i.e. portions) of such proteins encoded by nucleic acid molecules that are at least 18 nucleotides. Percent identity as used herein is determined using the Compare function by maximum matching within the program DNAsis Version 2.1 using default parameters.
Additional preferred flea HMT and/or HNC proteins of the present invention include proteins having an amino acid sequence encoded by a nucleic acid sequence of Table I and/or Table II, and proteins comprising homologues of a protein encoded by a nucleic acid sequence of Table I and/or Table II, wherein such a homologue comprises at least one epitope that elicits an immune response against a protein encoded by a nucleic acid sequence of Table I and/or Table 1I.
In one embodiment, a preferred flea HMT or HNC protein comprises an amino acid sequence of at least 6 amino acids, preferably at least 10 amino acids, preferably at least 15 amino acids, preferably at least 20 amino acids, preferably at least 25 amino acids, preferably at least 30 amino acids, preferably at least 35 amino acids, preferably at least 40 amino acids, preferably at least 50 amino acids, preferably at least 100 amino acids, preferably at least 200 amino acids, preferably at least 250 amino acids, preferably at least 300 amino acids, preferably at least 350 amino acids, and preferably at least 375 amino acids. In another embodiment, preferred flea HMT
and

-26-HNC proteins comprise full-length proteins, i.e., proteins encoded by full-length coding regions, or post-translationally modified proteins thereof, such as mature proteins from which initiating methionine and/or signal sequences or "pro"
sequences have been removed.
A fragment of an HMT and/or HNC protein of the present invention preferably comprises at least 5 amino acids, preferably at least 10 amino acids, preferably at least 15 amino acids, preferably at least 20 amino acids, preferably at least 25 amino acids, preferably at least 30 amino acids, preferably at least 35 amino acids, preferably at least 40 amino acids, preferably at least 45 amino acids, preferably at least 50 amino acids, preferably at least 55 amino acids, preferably at least 60 amino acids, preferably at least 65 amino acids, preferably at least 70 amino acids, preferably at least 75 amino acids, preferably at least 80 amino acids, preferably at least 85 amino acids, preferably at least 90 amino acids, preferably at least 95 amino acids, and preferably at least 100 amino acids in length.
In another embodiment, a preferred flea HMT and/or HNC protein of the present invention is encoded by a nucleic acid molecule comprising at least 15 nucleotides, preferably at least 18 nucleotides, preferably at least 20 nucleotides, preferably at least 25 nucleotides, preferably at least 30 nucleotides, preferably at least 40 nucleotides, preferably at least 50 nucleotides, preferably at least nucleotides, preferably at least 150 nucleotides, preferably at least 350 nucleotides, preferably at least 450 nucleotides, preferably at least 550 nucleotides, preferably at least 650 nucleotides, preferably at least 750 nucleotides, preferably at least 1000 nucleotides, and preferably at least 1100 nucleotides in length. In another embodiment, preferred flea HMT and HNC proteins of the present invention are encoded by nucleic acid molecules comprising apparently full-length HMT or HNC
coding regions respectively, i.e., nucleic acid molecules encoding an apparently full-length HMT or HNC proteins.
Preferred flea HMT and HNC proteins of the present invention can be used to develop inhibitors that, when administered to an animal in an effective manner, are capable of protecting that animal from flea infestation. In accordance with the present invention, the ability of an inhibitor of the present invention to protect an animal from _27_ flea infestation refers to the ability of that protein to, for example, treat, ameliorate and/or prevent infestation caused by fleas. In particular, the phrase "to protect an animal from flea infestation" refers to reducing the potential for flea population expansion on and around the animal (i.e., reducing the flea burden).
Preferably, the flea population size is decreased, optimally to an extent that the animal is no longer bothered by fleas. A host animal, as used herein, is an animal from which fleas can feed by attaching to and feeding through the skin of the animal. Fleas, and other ectoparasites, can live on a host animal for an extended period of time or can attach temporarily to an animal in order to feed. At any given time, a certain percentage of a flea population can be on a host animal whereas the remainder can be in the environment of the animal. Such an environment can include not only adult fleas, but also flea eggs and/or flea larvae. The environment can be of any size such that fleas in the environment are able to jump onto and off of a host animal. For example, the environment of an animal can include plants, such as crops, from which fleas infest an animal. As such, it is desirable not only to reduce the flea burden on an animal per se, but also to reduce the flea burden in the environment of the animal.
Suitable fleas to target include any flea that is essentially incapable of causing disease in an animal administered an inhibitor of the present invention. As such, fleas to target include any flea that produces a protein that can be targeted by an inhibitory compound that inhibits a flea HMT or HNC protein function, thereby resulting in the decreased ability of the parasite to cause disease in an animal. Preferred fleas to target include fleas of the following genera: Cte>zocep7ialides, Cyopsyllus, Diamahus (Oropsylla), Ec7zidhophaga, Nosopsyllus, Pulex, Tutzga, and XezZOpsylla, with those of the species Ctehocephalides cayzis, Cterzoceplzalides felis, Diamarzus mozztahus, Echid~zophaga galliz2acea, Nosopsyllus faciatus, Pulex irritayzs, Pulex simulazzs, TuYZga pe~cetrazzs and Xehopsylla cheopis being more preferred, with C. felis being preferred.
Such fleas are also preferred for the isolation of proteins or nucleic acid molecules of the present invention.
One embodiment of a flea HMT and/or HNC protein of the present invention is a fusion protein that includes a flea HMT and/or HNC protein-containing domain attached to one or more fusion segments. Suitable fusion segments for use with the present invention include, but are not limited to, segments that can: enhance a protein's stability; act as an immunopotentiator to enhance an immune response against a flea HMT and/or HNC protein; and/or assist in purification of a flea HMT and/or HNC
protein (e.g., by affinity chromatography). A suitable fusion segment can be a domain of any size that has the desired function (e.g., imparts increased stability, imparts increased immunogenicity to a protein, and/or simplifies purification of a protein).
Fusion segments can be joined to amino and/or carboxyl termini of the flea HMT-containing and/or HNC-containing domain of the protein and can be susceptible to cleavage in order to enable straight-forward recovery of a flea HMT and/or HNC
protein. Fusion proteins axe preferably produced by culturing a recombinant cell transformed with a fusion nucleic acid molecule that encodes a protein including the fusion segment attached to either the carboxyl and/or amino terminal end of an HMT-containing and/or HNC-containing domain. Preferred fusion segments include a metal binding domain (e.g., a poly-histidine segment); an immunoglobulin binding domain (e.g., Protein A; Protein G; T cell; B cell; Fc receptor or complement protein antibody-binding domains); a sugar binding domain (e.g., a maltose binding domain);
andlor a "tag" domain (e.g., at least a portion of J3-galactosidase, a strep tag peptide, a T7 tag peptide, a FlagTM peptide, or other domains that can be purified using compounds that bind to the domain, such as monoclonal antibodies). More preferred fusion segments include metal binding domains, such as a poly-histidine segment; a maltose binding domain; a strep tag peptide, such as that available from Biometra in Tampa, FL; and an S 10 peptide.
The present invention also includes mimetopes of flea HMT and/or HNC
proteins of the present invention. As used herein, a mimetope of a flea HMT
and/or HNC protein of the present invention refers to any compound that is able to mimic the activity of such an HMT and/or HNC protein, often because the mimetope has a structure that mimics the particular HMT and/or HNC protein. Mimetopes can be, but are not limited to: peptides that have been modified to decrease their susceptibility to degradation such as all-D retro peptides; anti-idiotypic and/or catalytic antibodies, or fragments thereof; non-proteinaceous immunogenic portions of an isolated protein (e.g., carbohydrate structures); and synthetic or natural organic molecules, including nucleic acids. Such mimetopes can be designed using computer-generated structures of proteins of the present invention. Mimetopes can also be obtained by generating random samples of molecules, such as oligonucleotides, peptides or other organic molecules, and screening such samples by affinity chromatography techniques using the corresponding binding partner.
Another embodiment of the present invention is an isolated nucleic acid molecule comprising a flea HMT and/or HNC nucleic acid molecule, i.e. a nucleic acid molecule that can be isolated from a HMT cDNA library, from a HNC cDNA
library, or from both libraries. As used herein, HMT and HNC nucleic acid molecules has the same meaning as HMT and/or HNC nucleic acid molecule. The identifying characteristics of such nucleic acid molecules are heretofore described. A
nucleic acid molecule of the present invention can include an isolated natural flea HMT
and/or HNC gene or a homologue thereof, the latter of which is described in more detail below. A nucleic acid molecule of the present invention can include one or more regulatory regions, full-length or partial coding regions, or combinations thereof. The minimal size of a nucleic acid molecule of the present invention is a size sufficient to allow the formation of a stable hybrid (i.e., hybridization under stringent hybridization conditions) with the complementary sequence of another nucleic acid molecule.
As such, the minimal size of a HMT and/or HNC nucleic acid molecule of the present invention is from 12 to 1 ~ nucleotides in length. Suitable and preferred fleas from which to isolate nucleic acid molecules of the present invention are disclosed herein.
Particularly preferred HMT and/or HNC nucleic acid molecules include C. felis HMT
and/or HNC nucleic acid molecules.
In accordance with the present invention, an isolated nucleic acid molecule is a nucleic acid molecule that has been removed from its natural milieu (i.e., that has been subjected to human manipulation) and can include DNA, RNA, or derivatives of either DNA or RNA. As such, "isolated" does not reflect the extent to which the nucleic acid molecule has been purified. Isolated flea HMT and/or HNC nucleic acid molecules of the present invention, or homologues thereof, can be isolated from a natural source or produced using recombinant DNA technology (e.g., polymerase chain reaction (PCR) amplification or cloning) or chemical synthesis. Isolated flea HMT and/or HNC

nucleic acid molecules, and homologues thereof, can include, for example, natural allelic variants and nucleic acid molecules modified by nucleotide insertions, deletions, substitutions, andlor inversions in a manner such that the modifications do not substantially interfere with the nucleic acid molecule's ability to encode a HMT
and/or HNC protein of the present invention.
A flea HMT andlor HNC nucleic acid molecule homologue can be produced using a number of methods known to those skilled in the art, see, for example, Sambrook et al., ibid., is incorporated by reference herein in its entirety.
For example, nucleic acid molecules can be modified using a variety of techniques including, but not limited to, classic mutagenesis and recombinant DNA techniques such as site-directed mutagenesis, chemical treatment, restriction enzyme cleavage, ligation of nucleic acid fragments, PCR amplification, synthesis of oligonucleotide mixtures and ligation of mixture groups to "build" a mixture of nucleic acid molecules, and combinations thereof. Nucleic acid molecule homologues can be selected by hybridization with flea HMT andlor HNC nucleic acid molecules or by screening the function of a protein encoded by the nucleic acid molecule (e.g., ability to elicit an immune response against at least one epitope of a flea HMT or HNC protein or to effect HMT or HNC
activity).
An isolated nucleic acid molecule of the present invention can include a nucleic acid sequence that encodes at least one flea HMT or HNC protein of the present invention, examples of such proteins being disclosed herein. Although the phrase "nucleic acid molecule" primarily refers to the physical nucleic acid molecule and the phrase "nucleic acid sequence" primarily refers to the sequence of nucleotides on the nucleic acid molecule, the two phrases can be used interchangeably, especially with respect to a nucleic acid molecule, or a nucleic acid sequence, being capable of encoding a flea HMT or HNC protein.
A preferred nucleic acid molecule of the present invention, when administered to an animal, is capable of protecting that animal from flea infestation. As will be disclosed in more detail below, such a nucleic acid molecule can be, or encode, an antisense RNA, a molecule capable of triple helix formation, a ribozyme, or other nucleic acid-based drug compound. In additional embodiments, a nucleic acid molecule of the present invention can encode a protective protein (e.g., an HMT or HNC protein of the present invention), the nucleic acid molecule being delivered to the animal, for example, by direct injection (i.e, as a genetic vaccine) or in a vehicle such as a recombinant virus vaccine or a recombinant cell vaccine.
In one embodiment of the present invention, a preferred flea HMT and/or HNC
nucleic acid molecule includes an isolated nucleic acid molecule that hybridizes under conditions that preferably allow less than or equal to 30% base pair mismatch, preferably under conditions that allow less than or equal to 25% base pair mismatch, preferably under conditions that allow less than or equal to 20% base pair mismatch, preferably under conditions that allow less than or equal to 15% base pair mismatch, preferably under conditions that allow less than or equal to 10% base pair mismatch and preferably under conditions that allow less than or equal to 5% base pair mismatch with a nucleic acid molecule of Table I and/or Table II and/or a nucleic acid molecule that is complementary to a nucleic acid molecule of Table I and/or Table II.
Another embodiment of the present invention includes a HMT and/or HNC
nucleic acid molecule, wherein said nucleic acid molecule hybridizes, in a solution comprising 1X SSC in the absence of helix destabilizing compounds, at a temperature of 47.5°C, to an isolated nucleic acid molecule a nucleic acid molecule of Table I or Table II and/or a nucleic acid molecule that is complementary to a nucleic acid molecule of Table I or Table II. Additional preferred nucleic acid molecules of the present invention include oligonucleotides of an isolated nucleic acid molecule, wherein said nucleic acid molecule hybridizes, in a solution comprising 1X SSC
in the absence of helix destabilizing compounds, at a temperature of 47.5°C, to an isolated nucleic acid molecule of Table I or Table II and/or a nucleic acid molecule that is complementary to a nucleic acid molecule of Table I or Table II, wherein said oligonucleotide comprises at least 18 nucleotides.
Additional preferred flea HMT and/or HNC nucleic acid molecules of the present invention include nucleic acid molecules comprising a nucleic acid sequence that is preferably at least 70%, preferably at least 75%, preferably at least 80%
preferably at least 85%, preferably at least 90%, and preferably at least 95%
identical to a nucleic acid sequence of Table I or Table II and/or a nucleic acid molecule that is complementary to a nucleic acid molecule of Table I or Table II. Also preferred are oligonucleotides of any of such nucleic acid molecules. Percent identity may be determined using the SeqLab sequence analysis software, using default parameters.
Another preferred nucleic acid molecule of the present invention includes at least a portion of nucleic acid sequence of Table I or Table II, as well as allelic variants of nucleic acid molecules having these nucleic acid sequences and homologues of nucleic acid molecules having these nucleic acid sequences; preferably such a homologue encodes or is complementary to a nucleic acid molecule that encodes at least one epitope that elicits an immune response against a protein encoded by a nucleic acid molecule of Table I and/or Table II. Such nucleic acid molecules can include nucleotides in addition to those included in the SEQ ID NOs, such as, but not limited to, a full-length gene, a full-length coding region, a nucleic acid molecule encoding a fusion protein, or a nucleic acid molecule encoding a multivalent protective compound.
In one embodiment, HMT and/or HNC nucleic acid molecule of the present invention encodes a protein that is at least 70%, preferably at least 75%, preferably at least 80%, preferably at least 85%, preferably at least 90%, preferably at least 95%, preferably at least 98%, and preferably at least 100% identical to a protein encoded by a nucleic acid molecule having a sequence of Table I and/or Table If.
In one embodiment, a HMT and/or HNC nucleic acid molecule of the present invention encodes a protein having an amino acid sequence that is at least 70%, preferably at least 75%, preferably at least 80%, preferably at least 85%, preferably at least 90%, preferably at least 95%, preferably at least 98%, and preferably at least 100% identical to a protein encoded by a nucleic acid molecule having a sequence of Table I and/or Table II. The present invention also includes a HMT
and/or HNC nucleic acid molecule encoding a protein having at least a portion of a protein encoded by a nucleic acid molecule having a sequence of Table I and/or Table II, as well as allelic variants of a nucleic acid molecule encoding a protein having these sequences, including nucleic acid molecules that have been modified to accommodate codon usage properties of the cells in which such nucleic acid molecules are to be expressed.

In another embodiment, a preferred flea HMT and/or HNC nucleic acid molecule of the present invention comprises a nucleic acid molecule comprising at least 15 nucleotides, preferably at least 18 nucleotides, preferably at least nucleotides, preferably at least 25 nucleotides, preferably at least 30 nucleotides, preferably at least 40 nucleotides, preferably at least 50 nucleotides, preferably at least 100 nucleotides, preferably at least 150 nucleotides, preferably at least 350 nucleotides, preferably at least 450 nucleotides, preferably at least 550 nucleotides, preferably at least 650 nucleotides, preferably at least 750 nucleotides, preferably at least 1000 nucleotides, and preferably at least 1100 nucleotides in length.
In another embodiment, a preferred flea HMT and/or HNC nucleic acid molecule encodes a protein comprising at least 5 amino acids, preferably at least 6 amino acids, preferably at least 10 amino acids, preferably at least 15 amino acids, preferably at least 20 amino acids, preferably at least 25 amino acids, preferably at least 30 amino acids, preferably at least 40 amino acids, preferably at least 50 amino acids, preferably at least 100 amino acids, preferably at least 150 amino acids, preferably at least 200 amino acids, preferably at least 300 amino acids, preferably at least 350 amino acids, and preferably at least 350 amino acids in length.
In another embodiment, a preferred flea HMT and/or HNC nucleic acid molecule of the present invention comprises an apparently full-length HMT
and/or HNC coding region, i.e., the preferred nucleic acid molecule encodes an apparently full-length HMT and/or HNC protein, or a post-translationally modified protein thereof. In one embodiment, a preferred HMT and/or HNC nucleic acid molecule of the present invention encodes a mature protein.
Knowing the nucleic acid sequences of certain flea HMT and/or HNC nucleic acid molecules of the present invention allows one skilled in the art to, for example, (a) make copies of those nucleic acid molecules, (b) obtain nucleic acid molecules including at least a portion of such nucleic acid molecules (e.g., nucleic acid molecules including full-length genes, full-length coding regions, regulatory control sequences, truncated coding regions), and (c) obtain other flea HMT and/or HNC nucleic acid molecules. Such nucleic acid molecules can be obtained in a variety of ways including screening appropriate expression libraries with antibodies of the present invention;

traditional cloning techniques using oligonucleotide probes of the present invention to screen appropriate libraries; and PCR amplification of appropriate libraries or DNA
using oligonucleotide primers of the present invention. Preferred libraries to screen or from which to amplify nucleic acid molecules include flea 1St instar larvae;
3'a instar larvae, wandering larvae, prepupal larvae, pupae and whole adult flea cDNA
libraries as well as genomic DNA libraries. Similarly, preferred DNA sources to screen or from which to amplify nucleic acid molecules include flea prepupal cDNA, adult cDNA and genomic DNA. Techniques to clone and amplify genes are disclosed, for example, in Sambrook et al., ibid.
The present invention also includes nucleic acid molecules that are oligonucleotides capable of hybridizing, under stringent hybridization conditions, with complementary regions of other, preferably longer, nucleic acid molecules of the present invention such as those comprising C. felis HMT and/or HNC nucleic acid molecules or other flea HMT and/or HNC nucleic acid molecules.
Oligonucleotides of the present invention can be RNA, DNA, or derivatives of either. The minimum size of such oligonucleotides is the size required for formation of a stable hybrid between an oligonucleotide and a complementary sequence on a nucleic acid molecule of the present invention. A preferred oligonucleotide of the present invention has a maximum size of preferably 100 to 200 nucleotides. The present invention includes oligonucleotides that can be used as, for example, probes to identify nucleic acid molecules, primers to produce nucleic acid molecules, or therapeutic reagents to inhibit flea HMT and/or HNC protein production or activity (e.g., as antisense-, triplex formation-, ribozyme- and/or RNA drug-based reagents). The present invention also includes the use of such oligonucleotides to protect animals from disease using one or more of such technologies. Appropriate oligonucleotide-containing therapeutic compositions can be administered to an animal using techniques known to those skilled in the art.
One embodiment of the present invention includes a recombinant vector, which includes at least one isolated nucleic acid molecule of the present invention, inserted into any vector capable of delivering the nucleic acid molecule into a host cell. Such a vector contains heterologous nucleic acid sequences, that is nucleic acid sequences that are not naturally found adj acent to nucleic acid molecules of the present invention and that preferably are derived from a species other than the species from which the nucleic acid molecules) are derived. The vector can be either RNA or DNA, either prokaryotic or eukaryotic, and typically is a virus or a plasmid. Recombinant vectors can be used in the cloning, sequencing, andlor otherwise manipulating of flea HMT
and/or HNC nucleic acid molecules of the present invention.
One type of recombinant vector, referred to herein as a recombinant molecule, comprises a nucleic acid molecule of the present invention operatively linked to an expression vector. The phrase operatively linked refers to insertion of a nucleic acid molecule into an expression vector in a manner such that the molecule is able to be expressed when transformed into a host cell. As used herein, an expression vector is a DNA or RNA vector that is capable of transforming a host cell and of effecting expression of a specified nucleic acid molecule. Preferably, the expression vector is also capable of replicating within the host cell. Expression vectors can be either prokaryotic or eukaryotic, and are typically viruses or plasmids. Expression vectors of the present invention include any vectors that function (i.e., direct gene expression) in recombinant cells of the present invention, including in bacterial, fungal, parasite, insect, other animal, and plant cells. Preferred expression vectors of the present invention can direct gene expression in bacterial, yeast, insect and mammalian cells, and preferably in the cell types disclosed herein.
In particular, expression vectors of the present invention contain regulatory sequences such as transcription control sequences, translation control sequences, origins of replication, and other regulatory sequences that are compatible with the recombinant cell and that control the expression of nucleic acid molecules of the present invention. In particular, recombinant molecules of the present invention include transcription control sequences. Transcription control sequences are sequences that control the initiation, elongation, and termination of transcription.
Particularly important transcription control sequences are those which control transcription initiation, such as promoter, enhancer, operator and repressor sequences.
Suitable transcription control sequences include any transcription control sequence that can function in at least one of the recombinant cells of the present invention. A variety of such transcription control sequences are known to those skilled in the art.
Preferred transcription control sequences include those that function in bacterial, yeast, or insect and mammalian cells, such as, but not limited to, tac, lac, tfp, trc, oxy-pro, ompllpp, rrnB, bacteriophage lambda (such as lambda pL and lambda pR and fusions that include such promoters), bacteriophage T7, T7lac, bacteriophage T3, bacteriophage SP6, bacteriophage SP01, metallothionein, alpha-mating factor, Picl2ia alcohol oxidase, alphavirus subgenoxnic promoter, antibiotic resistance gene, baculovirus, Heliothis zea insect virus, vaccinia virus, heipesvirus, raccoon poxvirus, other poxvirus, adenovirus, cytomegalovirus (such as immediate early promoter), simian virus 40, retrovirus, actin, retroviral long terminal repeat, Rous sarcoma virus, heat shock, phosphate and nitrate transcription control sequences as well as other sequences capable of controlling gene expression in prokaryotic or eukaryotic cells. Additional suitable transcription control sequences include tissue-specific promoters and enhancers as well as lymphokine-inducible promoters (e.g., promoters inducible by interferons or interleukins).
Transcription control sequences of the present invention can also include naturally occurring transcription control sequences naturally associated with fleas, such as C.
felis transcription control sequences.
Suitable and preferred nucleic acid molecules to include in recombinant vectors of the present invention are as disclosed herein. Preferred nucleic acid molecules to include in recombinant vectors, and particularly in recombinant molecules, include nucleic acid molecules having a sequence of Table I andlor Table II.
Recombinant molecules of the present invention may also (a) contain secretory signals (i.e., signal segment nucleic acid sequences) to enable an expressed flea protein of the present invention to be secreted from the cell that produces the protein and/or (b) contain fusion sequences which lead to the expression of nucleic acid molecules of the present invention as fusion proteins. Examples of suitable signal segments include any signal segment capable of directing the secretion of a protein of the present invention. Preferred signal segments include, but are not limited to, tissue plasminogen activator (t-PA), interferon, interleukin, growth hormone, histoeompatibility and viral envelope glycoprotein signal segments. Suitable fusion segments encoded by fusion segment nucleic acids are disclosed herein. In addition, a WO 03/031577 PCT/US02/31878....

nucleic acid molecule of the present invention can be joined to a fusion segment that directs the encoded protein to the proteosome, such as a ubiquitin fusion segment.
Eukaryotic recombinant molecules may also include intervening and/or untranslated sequences surrounding andlor within the nucleic acid sequences of nucleic acid molecules of the present invention.
Another embodiment of the present invention includes a recombinant cell comprising a host cell transformed with one or more recombinant molecules of the present invention. Transformation of a nucleic acid molecule into a cell can be accomplished by any method by which a nucleic acid molecule can be inserted into the cell. Transformation techniques include, but are not limited to, transfection, electroporation, microinjection, lipofection, adsorption, and protoplast fusion. A
recombinant cell may remain unicellular or may grow into a tissue, organ or a multicellular organism. It is to be noted that a cell line refers to any recombinant cell of the present invention that is not a transgenic animal. Transformed nucleic acid molecules of the present invention can remain extrachromosomal or can integrate into one or more sites within a chromosome of the transformed (i.e., recombinant) cell in such a manner that their ability to be expressed is retained. PrefeiTed nucleic acid molecules with which to transform a cell include G felis HMT and HNC nucleic acid molecules disclosed herein. Preferred nucleic acid molecules with which to transform a cell include nucleic acid molecules having a sequence of Table I and/or Table II.
Suitable host cells to transform include any cell that can be transformed with a nucleic acid molecule of the present invention. Host cells can be either untransformed cells or cells that are already transformed with at least one nucleic acid molecule (e.g., nucleic acid molecules encoding one or more proteins of the present invention andlor other proteins useful in the production of multivalent vaccines). Host cells of the present invention either can be endogenously (i.e., naturally) capable of producing flea HMT andlor HNC proteins of the present invention or can be capable of producing such proteins after being transformed with at least one nucleic acid molecule of the present invention. Host cells of the present invention can be any cell capable of producing at least one protein of the present invention, and include bacterial, fungal (including yeast), parasite (including helminth, protozoa and ectoparasite), other insect, other animal and plant cells. Preferred host cells include bacterial, mycobacterial, yeast, insect and mammalian cells. More preferred host cells include Salfraonella, Eschericlzia, Bacillus, Caulobacter-, Listeria, Sacclzaro~vyces, Piclzia, Spodoptera, Mycobacteria, Trichoplusia, BHK (baby hamster kidney) cells, MDCK
cells (Madin-Darby canine kidney cell line), CRFK cells (Crandell feline kidney cell line), CV-1 cells (African monkey kidney cell line used, for example, to culture raccoon poxvirus), COS (e.g., COS-7) cells, and Vero cells. Particularly preferred host cells are Eschericl2ia coli, including E. coli K-12 derivatives; Salynorcella typl2i;
Salr~2or~ella typhiri2uriuf~z, including attenuated strains such as UK-1 x3987 and SR-11 x4072; Caulobacter; Pichia; Spodoptera frugipef°da; Trichoplusia jai;
BHK cells;
MDCK cells; CRFK cells; CV-1 cells; COS cells; Vero cells; and non-tumorigenic mouse myoblast G8 cells (e.g., ATCC CRL 1246). Additional appropriate mammalian cell hosts include other kidney cell lines, other fibroblast cell lines (e.g., human, murine or chicken embryo fibroblast cell lines), myeloma cell lines, Chinese hamster ovary cells, mouse NIH/3T3 cells, LMTK31 cells and/or HeLa cells. In one embodiment, the proteins may be expressed as heterologous proteins in myeloma cell lines employing immunoglobulin promoters.
A recombinant cell is preferably produced by transforming a host cell with one or more recombinant molecules, each comprising one or more nucleic acid molecules of the present invention operatively linked to an expression vector containing one or more transcription control sequences, examples of which are disclosed herein.
The phrase operatively linked refers to insertion of a nucleic acid molecule into an expression vector in a manner such that the molecule is able to be expressed when transformed into a host cell.
A recombinant cell of the present invention includes any cell transformed with at least one of any nucleic acid molecule of the present invention. Suitable and preferred nucleic acid molecules as well as suitable and preferred recombinant molecules with which to transfer cells are disclosed herein.
Recombinant cells of the present invention can also be co-transformed with one or more recombinant molecules including flea HMT and/or HNC nucleic acid molecules encoding one or more proteins of the present invention and one or more other nucleic acid molecules encoding other protective compounds, as disclosed herein (e.g., to produce multivalent vaccines).
Recombinant DNA technologies can be used to improve expression of transformed nucleic acid molecules by manipulating, for example, the number of copies of the nucleic acid molecules within a host cell, the efficiency with which those nucleic acid molecules are transcribed, the efficiency with which the resultant transcripts are translated, and the efficiency of post-translational modifications.
Recombinant techniques useful for increasing the expression of nucleic acid molecules of the present invention include, but are not limited to, operatively linking nucleic acid molecules to high-copy number plasmids, integration of the nucleic acid molecules into one or more host cell chromosomes, addition of vector stability sequences to plasmids, substitutions or modifications of transcription control signals (e.g., promoters, operators, enhancers), substitutions or modifications of translational control signals (e.g., ribosome binding sites, Shine-Dalgarno sequences), modification of nucleic acid molecules of the present invention to correspond to the codon usage of the host cell, deletion of sequences that destabilize transcripts, and use of control signals that temporally separate recombinant cell growth from recombinant enzyme production during fermentation. The activity of an expressed recombinant protein of the present invention may be improved by fragmenting, modifying, or derivatizing nucleic acid molecules encoding such a protein.
Isolated flea HMT and/or HNC proteins of the present invention can be produced in a variety of ways, including production and recovery of natural proteins, production and recovery of recombinant proteins, and chemical synthesis of the proteins. In one embodiment, an isolated protein of the present invention is produced by culturing a cell capable of expressing the protein under conditions effective to produce the protein, and recovering the protein. A preferred cell to culture is a recombinant cell of the present invention. Effective culture conditions include, but are not limited to, effective media, bioreactor, temperature, pH and oxygen conditions that permit protein production. An effective, medium refers to any medium in which a cell is cultured to produce a flea HMT and/or HNC protein of the present invention.
Such medium typically comprises an aqueous medium having assimilable carbon, nitrogen and phosphate sources, and appropriate salts, minerals, metals and other nutrients, such as vitamins. Cells of the present invention can be cultured in conventional fermentation bioreactors, shake flasks, test tubes, microtiter dishes, and petri plates.
Culturing can be carried out at a temperature, pH and oxygen content appropriate for a recombinant cell. Such culturing conditions are within the expertise of one of ordinary skill in the art. Examples of suitable conditions are included in the Examples section.
Depending on the vector and host system used for production, resultant proteins of the present invention may either remain within the recombinant cell; be secreted into the.fermentation medium; be secreted into a space between two cellular membranes, such as the periplasmic space in E. coli; or be retained on the outer sunace of a cell or viral membrane.
The phrase "recovering the protein", as well as similar phrases, refers to collecting the whole fermentation medium containing the protein and need not imply additional steps of separation or purification. Proteins of the present invention can be purified using a variety of standard protein purification techniques, such as, but not limited to, affinity chromatography, ion exchange chromatography, filtration, electrophoresis, hydrophobic interaction chromatography, gel filtration chromatography, reverse phase chromatography, concanavalin A chromatography, chromatofocusing and differential solubilization. Proteins of the present invention are preferably retrieved in "substantially pure" form. As used herein, "substantially pure"
refers to a purity that allows for the effective use of the protein as a therapeutic composition or diagnostic. A therapeutic composition for animals, for example, should exhibit no substantial toxicity and preferably should be capable of stimulating the production of antibodies in a treated animal.
The present invention also includes isolated (i.e., removed from their natural milieu) antibodies that selectively bind to a flea HMT and/or HNC protein of the present invention or a mimetope thereof (e.g., anti-C. felis H1VIT or HNC
antibodies).
As used herein, the term "selectively binds to" an HMT andlor HNC protein refers to the ability of antibodies of the present invention to preferentially bind to specified proteins and mimetopes thereof of the present invention. Binding can be measured using a variety of methods standard in the art including enzyme immunoassays (e.g., ELISA), immunoblot assays, etc.; see, for example, Sambrook et al., ibid., and Harlow, et al., 1988, AfZtibodies, a Laboratory Mafaual, Cold Spring Harbor Labs Press;
Harlow et al., ibid., is incorporated by reference herein in its entirety. An anti-HMT or anti-HNC antibody of the present invention preferably selectively binds to a flea HMT
or HNC protein respectively in such a way as to inhibit the function of that protein.
Isolated antibodies of the present invention can include antibodies in serum, or antibodies that have been purified to varying degrees. Antibodies of the present invention can be polyclonal or monoclonal, or can be functional equivalents such as antibody fragments and genetically-engineered antibodies, including single chain antibodies or chimeric antibodies that can bind to one or more epitopes.
A preferred method to produce antibodies of the present invention includes (a) administering to an animal an effective amount of a protein, peptide or mimetope thereof of the present invention to produce the antibodies and (b) recovering the antibodies. In another method, antibodies of the present invention are produced recombinantly using techniques as heretofore disclosed to produce HMT and/or HNC
proteins of the present invention. Antibodies raised against defined proteins or mimetopes can be advantageous because such antibodies are not substantially contaminated with antibodies against other substances that might otherwise cause interference in a diagnostic assay or side effects if used in a therapeutic composition.
Antibodies of the present invention have a variety of potential uses that are within the scope of the present invention. For example, such antibodies can be used (a) as therapeutic compounds to passively immunize an animal in order to protect the animal from fleas susceptible to treatment by such antibodies and/or (b) as tools to screen expression libraries and/or to recover desired proteins of the present invention from a mixture of proteins and other contaminants. Furthermore, antibodies of the present invention can be used to target cytotoxic agents to fleas in order to directly kill such fleas. Targeting can be accomplished by conjugating (i.e., stably joining) such antibodies to the cytotoxic agents using techniques known to those skilled in the art.
Suitable cytotoxic agents are known to those skilled in the art.
One embodiment of the present invention is a therapeutic composition that, when administered to an animal susceptible to flea infestation, is capable of protecting that animal from flea infestation. Therapeutic compositions of the present invention include at least one of the following protective molecules: an isolated flea HMT and/or HNC protein; a mimetope of an isolated flea HMT and/or HNC protein; an isolated flea HMT and/or HNC nucleic acid molecule; and/or a compound derived from said isolated flea HMT and/or HNC protein that inhibits HMT and/or HNC protein activity.
A therapeutic composition of the present invention can further comprise a component selected from the group of an excipient, a carrier, and/or an adjuvant; these components are described further herein. As used herein, a protective molecule or protective compound refers to a compound that, when administered to an animal in an effective manner, is able to treat, ameliorate, and/or prevent flea infestation. Preferred fleas to target are heretofore disclosed. One example of a protective molecule is a vaccine, such as, but not limited to, a naked nucleic acid vaccine, a recombinant virus vaccine, a recombinant cell vaccine, and a recombinant protein vaccine.
Another example of a protective molecule is a compound that inhibits HMT and/or HNC
protein activity, such as an isolated antibody that selectively binds to a flea HMT
and/or HNC protein, a substrate analog of a flea HMT and/or HNC protein, anti-sense-triplex formation-, ribozyme-, and/or RNA drug-based compounds, or other inorganic or organic molecules that inhibit HMT and/or HNC protein activity. Inhibiting flea HMT and/or HNC protein activity can refer to the ability of a compound to reduce the activity of flea HMT and/or HNC proteins. Inhibiting flea HMT and/or HNC
protein activity can also refer to the ability of a compound to reduce the amount of flea HMT
and/or HNC protein in a flea.
Another embodiment of the present invention includes a method to reduce a flea infestation in an animal susceptible to flea infestation. Such a method includes the step of administering to the animal a therapeutic molecule comprising a protective compound selected from the group consisting of (a) an isolated flea HMT and/or HNC
protein; (b) a mimetope of an isolated flea HMT and/or HNC protein; (c) an isolated flea HMT and/or HNC nucleic acid molecule; and (d) a compound derived from an isolated flea HMT and/or HNC protein that inhibits HMT and/or HNC protein activity.

Therapeutic compositions of the present invention can be administered to any animal susceptible to flea infestation, preferably to mammals, and preferably to dogs, cats, humans, ferrets, horses, cattle, sheep, and other pets, economic food animals, work animals andlor zoo animals. Preferred animals to protect against flea infestation include dogs, cats, humans, and ferrets, with dogs and cats being particularly preferred.
As used herein, the term derived, or the term derived from, refers to a peptide, antibody, mimetope, nucleic acid molecule, or other compound that was obtained from or obtained using a flea HMT and/or HNC protein or nucleic acid molecule of the present invention. Methods to obtain derivatives from a HMT and/or HNC
molecule of the present invention are known in the art, and as such include, but are not limited to molecular modeling of HMT and/or HNC proteins to determine active sites, i.e.
sites that interact with other molecules, and predicting from these active sites smaller fragments and/or mimetopes that retain and/or mimic these active sites, thereby inhibiting HMT and/or HNC protein activity; screening of peptide or small chemical compound libraries against HMT and/or HNC proteins of the present invention;
and screening of polyclonal or monoclonal antibodies to find antibodies that specifically bind HMT and/or HNC proteins of the present invention.
An HMT andlor HNC protein inhibitor of the present invention is identified by its ability to bind to, modify, or otherwise interact with, a flea HMT and/or HNC
protein, thereby inhibiting the activity of HMT and/or HNC proteins. Suitable inhibitors of HMT and/or HNC protein activity are compounds that inhibit HMT
and/or HNC protein activity in at least one of a variety of ways: (a) by binding to or otherwise interacting with or otherwise modifying HMT andlor HNC protein sites; (b) by binding to the HMT and/or HNC protein and thus reducing the availability of the HMT and/or HNC protein in solution; and (c) by interacting with other regions of the HMT and/or HNC protein to inhibit HMT and/or HNC protein activity, for example, by allosteric interaction.
Flea HMT and/or HNC protein inhibitors can be used directly as compounds in compositions of the present invention to treat animals as long as such compounds are not harmful to host animals being treated. Preferred HMT andlor HNC protein inhibitors of the present invention include, but are not limited to, flea HMT
and/or HNC protein substrate analogs, and other molecules that bind to a flea HMT
and/or HNC proteins (e.g., to an allosteric site) in such a manner that the activity of the flea HMT and/or HNC protein is inhibited. An HMT and/or HNC protein substrate analog refers to a compound that interacts with (e.g., binds to, associates with, modifies) the active site of a HMT and/or HNC protein. A preferred HMT and/or HNC protein substrate analog inhibits HMT and/or HNC protein activity. HMT and/or HNC
protein substrate analogs can be of any inorganic or organic composition. HMT and/or HNC
protein substrate analogs can be, but need not be, structurally similar to a HMT and/or HNC protein natural substrate as long as they can interact with the active site of that HMT and/or HNC protein. HMT and/or HNC protein substrate analogs can be designed using computer-generated structures of HMT and/or HNC proteins of the present invention or computer structures of HMT and/or HNC protein's natural substrates. Preferred sites to model include one or more of the active sites of HMT
and/or HNC protein. Substrate analogs can also be obtained by generating random samples of molecules, such as oligonucleotides, peptides, peptidomimetic compounds, or other inorganic or organic molecules, and screening such samples for their ability to interfere with interaction between HMT and/or HNC proteins and their substrates, e.g.
by affinity chromatography techniques. A preferred HMT and/or HNC protein substrate analog is a HMT and/or HNC protein mimetic compound, i.e., a compound that is structurally and/or functionally similar to a natural substrate of a HMT and/or HNC protein of the present invention, particularly to the region of the substrate that interacts with the HMT and/or HNC protein active site, but that inhibits HMT
and/or HNC protein activity upon interacting with the HMT and/or HNC protein active site.
The present invention also includes a therapeutic composition comprising at least one protective molecule of the present invention in combination with at least one additional compound protective against one or more infectious agents.
In one embodiment, a therapeutic composition of the present invention can be used to protect an animal from flea infestation by administering such composition to a flea in order to prevent infestation. Such administration to the flea andlor animal could be oral, or by application to the animal's body surface (e.g. topical spot-on, or spraying onto the animal), or by application to the environment (e.g., spraying).

Examples of such compositions include, but are not limited to, transgenic vectors capable of producing at least one therapeutic composition of the present invention. In another embodiment a flea can ingest therapeutic compositions, or products thereof, present on the surface of or in the blood of a host animal that has been administered a therapeutic composition of the present invention.
In accordance with the present invention, a host animal (i.e., an animal that is or is capable of being infested with fleas) is treated by administering to the animal a therapeutic composition of the present invention in such a manner that the composition itself (e.g., a HMT and/or HNC protein, a HMT and/or HNC nucleic acid molecule, a HMT and/or HNC protein inhibitor, a HMT and/or HNC protein synthesis suppressor (i.e., a compound that decreases the production or half life of a HMT and/or HNC
protein in fleas), a HMT and/or HNC protein rnimetope, or a anti-HMT and/or HNC
antibody) or a product generated by the animal in response to administration of the composition (e.g., antibodies produced in response to administration of a flea HMT
and/or HNC protein or nucleic acid molecule, or conversion of an inactive inhibitor "prodrug" to an active HMT and/or HNC protein inhibitor) ultimately enters the flea.
A host animal is preferably treated in such a way that the compound or product thereof is present on the body surface of the animal or enters the blood stream of the animal.
Fleas are then exposed to the composition or product when they feed from the animal.
For example, flea HMT and/or HNC protein inhibitors administered to an animal are administered in such a way that the inhibitors enter the blood, stream of the animal, where they can be taken up by feeding fleas.
The present invention also includes the ability to reduce larval flea infestation in that when fleas feed from a host animal that has been administered a therapeutic composition of the present invention, at least a portion of compounds of the present invention, or products thereof, in the blood taken up by the fleas are excreted by the fleas in feces, which is subsequently ingested by flea larvae. In particular, it is of note that flea larvae obtain most, if not all, of their nutrition from flea feces.
In accordance with the present invention, reducing HMT and/or HNC protein activity in a flea can lead to a number of outcomes that reduce flea burden on treated animals and their surrounding environments. Such outcomes include, but are not limited to, (a) reducing the viability of fleas that feed from the treated animal, (b) reducing the fecundity of female fleas that feed from the treated animal, (c) reducing the reproductive capacity of male fleas that feed from the treated animal, (d) reducing the viability of eggs laid by female fleas that feed from the treated animal, (e) altering the blood feeding behavior of fleas that feed from the treated animal (e.g., fleas take up less volume per feeding or feed less frequently), (f) reducing the viability of flea larvae, for example due to the feeding of larvae from feces of fleas that feed from the treated animal, (g) altering the development of flea larvae (e.g., by decreasing feeding behavior, inhibiting growth, inhibiting (e.g., slowing or blocking) molting, and/or otherwise inhibiting maturation to adults), and/or (h) altering or decreasing the ability of fleas or flea larvae to digest a blood meal.
In order to protect an animal from flea infestation, a therapeutic composition of the present invention is administered to the animal in an effective manner such that the composition is capable of protecting that animal from flea infestation.
Therapeutic compositions of the present invention can be administered to animals prior to infestation in order to prevent infestation (i.e., as a preventative vaccine) and/or can be administered to animals after infestation. For example, proteins, mimetopes thereof, and antibodies thereof can be used as immunotherapeutic agents.
Therapeutic compositions of the present invention can be formulated in an excipient that the animal to be treated can tolerate. Examples of such excipients include water, saline, Ringer's solution, dextrose solution, Hank's solution, and other aqueous physiologically balanced salt solutions. Nonaqueous vehicles, such as fixed oils, sesame oil, ethyl oleate, or triglycerides may also be used. Other useful formulations include suspensions containing viscosity enhancing agents, such as sodium carboxymethylcellulose, sorbitol, or dextran. Excipients can also contain minor amounts of additives, such as substances that enhance isotonicity and chemical stability. Examples of buffers include phosphate buffer, bicarbonate buffer and Tris buffer, while examples of preservatives include thimerosal, or o-cresol, formalin and benzyl alcohol. Standard formulations can either be liquid injectables or solids which can be taken up in a suitable liquid as a suspension or solution for injection. Thus, in a non-liquid formulation, the excipient can comprise dextrose, human serum albumin, preservatives, etc., to which sterile water or saline can be added prior to administr ation.
In one embodiment of the present invention, a therapeutic composition can include an adjuvant. Adjuvants are agents that are capable of enhancing the immune response of an animal to a specific antigen. Suitable adjuvants include, but are not limited to, cytokines, chemokines, and compounds that induce the production of cytokines and chemokines (e.g., granulocyte macrophage colony stimulating factor (GM-CSF), Flt-3 ligand, granulocyte colony stimulating factor (G-CSF), macrophage colony stimulating factor (M-CSF), colony stimulating factor (CSF), erythropoietin (EPO), interleukin 2 (IL-2), interleukin-3 (IL-3), interleukin 4 (1L-4), interleukin 5 (IL
5), interleukin 6 (IL-6), interleukin 7 (IL-7), interleukin 8 (IL-8), interleukin 10 (IL
10), interleukin 12 (IL-12), interferon gamma, interferon gamma inducing factor I
(IGIF), transforming growth factor beta, RANTES (regulated upon activation, normal T cell expressed and presumably secreted), macrophage inflammatory proteins (e.g., MIP-1 alpha and MIP-1 beta), and Leishmania elongation initiating factor (LEIF));
bacterial components (e.g., endotoxins, in particular superantigens, exotoxins and cell wall components); aluminum-based salts; calcium-based salts; silica;
polynucleotides;
toxoids; serum proteins, viral coat proteins; block copolymer adjuvants (e.g., Hunter's TitermaxTM adjuvant (VaxcelTM, Inc. Norcross, GA), Ribi adjuvants (Ribi ImmunoChem Research, Inc., Hamilton, MT); and saponins and their derivatives (e.g., Quil A (Superfos Biosector A/S, Denmark). Protein adjuvants of the present invention can be delivered in the form of the protein themselves or of nucleic acid molecules encoding such proteins using the methods described herein.
W one embodiment of the present invention, a therapeutic composition can include a carrier. Carriers include compounds that increase the half-life of a therapeutic composition in the treated animal. Suitable caiTiers include, but are not limited to, polymeric controlled release vehicles, biodegradable implants, liposomes, bacteria, viruses, other cells, oils, esters, and glycols.
One embodiment of the present invention is a controlled release formulation that is capable of slowly releasing a composition of the present invention into an animal. As used herein, a controlled release formulation comprises a composition of the present invention in a controlled release vehicle. Suitable controlled release vehicles include, but are not limited to, biocompatible polymers, other polymeric matrices, capsules, microcapsules, microparticles, bolus preparations, osmotic pumps, diffusion devices, liposomes, lipospheres, and transdermal delivery systems.
Other controlled release formulations of the present invention include liquids that, upon administration to an animal, form a solid or a gel ire situ. Preferred controlled release formulations are biodegradable (i.e., bioerodible).
A preferred controlled release formulation of the present invention is capable of releasing a composition of the present invention into the blood of the treated animal at a constant rate sufficient to attain therapeutic dose levels of the composition to protect an animal from flea infestation. The therapeutic composition is preferably released over a period of time ranging from about 1 to about 12 months. A
controlled release formulation of the present invention is capable of effecting a treatment preferably for at least 1 month, preferably for at least 3 months, preferably for at least 6 months, preferably for at least 9 months, and preferably for at least 12 months.
Acceptable protocols to administer therapeutic compositions in an effective manner include individual dose size, number of doses, frequency of dose administration, and mode of administration. Determination of such protocols can be ' accomplished by those skilled in the art. A suitable single dose is a dose that is capable of protecting an animal fiom disease when administered one or more times over a suitable time period. For example, a preferred single dose of a protein, mimetope or antibody therapeutic composition, including a recombinant protein vaccine, is from about 1 microgram (pg) to about 10 milligrams (mg) of the therapeutic composition per kilogram body weight of the animal. Booster vaccinations can be administered from about 2 weeks to several years after the original administration. Booster administrations preferably are administered when the immune response of the animal becomes insufficient to protect the animal from disease. A
preferred administration schedule is one in which from about 10 ~g to about 1 mg of the therapeutic composition per kg body weight of the animal is administered from about one to about two times over a time period of from about 2 weeks to about months. Modes of administration can include, but are not limited to, subcutaneous, intradermal, intravenous, intranasal, oral, transdermal, intraocular, intranasal, conjunctival, and intramuscular routes. Methods of administration for other therapeutic compounds can be determined by one skilled in the art, and may include administration of a therapeutic composition one or more times, on a daily, weekly, monthly or yearly regimen; routes of administration can be determined by one skilled in the art, and may include any route. A preferred route of administration of an inhibitory compound when administering to fleas is a topical, or "spot-on"
formulation administered to the body surface of the animal, so that a flea would encounter the inhibitory compound when attached to the animal; another preferred route of administration of an inhibitory compound is an oral formulation that, when fed to an animal, would enter the bloodstream of the animal, which would then be transferred to a flea while feeding from the animal.
A recombinant protein vaccine of the present invention comprises a recombinantly-produced flea HMT and/or HNC protein of the present invention that is administered to an animal according to a protocol that results in the animal producing a sufficient immune response to protect itself from a flea infestation. Such protocols can be determined by those skilled in the art.
According to one embodiment, a nucleic acid molecule of the present invention can be administered to an animal in a fashion to enable expression of that nucleic acid molecule into a protective protein or protective RNA (e.g., antisense RNA, ribozyme, triple helix forms or RNA drug) in the animal. Nucleic acid molecules can be delivered to an animal in a variety of methods including, but not limited to, (a) administering a naked (i.e., not packaged in a viral coat or cellular membrane) nucleic acid as a genetic vaccine (e.g., as naked DNA or RNA molecules, such as is taught, for example in Wolff et al., 1990, Science 247, 1465-1468) or (b) administering a nucleic acid molecule packaged as a recombinant virus vaccine or as a recombinant cell vaccine (i.e., the nucleic acid molecule is delivered by a viral or cellular vehicle).
A genetic (i.e., naked nucleic acid) vaccine of the present invention includes a nucleic acid molecule of the present invention and preferably includes a recombinant molecule of the present invention that preferably is replication, or otherwise amplification, competent. A genetic vaccine of the present invention can comprise one or more nucleic acid molecules of the present invention in the form of, for example, a dicistronic recombinant molecule. Preferred genetic vaccines include at least a portion of a viral genome, i.e., a viral vector. Preferred viral vectors include those based on alphaviruses, poxviruses, adenoviruses, herpesviruses, picornaviruses, and retroviruses, with those based on alphaviruses, such as sindbis or Semliki forest virus, species-specific herpesviruses and poxviruses being particularly preferred.
Any suitable transcription control sequence can be used, including those disclosed as suitable for protein production. Particularly preferred transcription control sequences include cytomegalovirus immediate early (preferably in conjunction with Intron-A), Rous sarcoma virus long terminal repeat, and tissue-specific transcription control sequences, as well as transcription control sequences endogenous to viral vectors if viral vectors are used. The incorporation of a "strong" polyadenylation signal is also preferred.
Genetic vaccines of the present invention can be administered in a variety of ways, with intramuscular, subcutaneous, intradermal, transdermal, conjunctival, intraocular, intranasal and oral routes of administration being preferred. A
preferred single dose of a genetic vaccine ranges from about 1 nanogram (ng) to about 600 fig, depending on the route of administration andlor method of delivery, as can be determined by those skilled in the art. Suitable delivery methods include, for example, by injection, as drops, aerosolized and/or topically. Genetic vaccines of the present invention can be contained in an aqueous excipient (e.g., phosphate buffered saline) alone or in a carrier (e.g., lipid-based vehicles).
A recombinant virus vaccine of the present invention includes a recombinant molecule of the present invention that is packaged in a viral coat and that can be expressed in an animal after administration. Preferably, the recombinant molecule is packaging- or replication-deficient and/or encodes an attenuated virus. A
number of recombinant viruses can be used, including, but not limited to, those based on alphaviruses, poxviruses, adenoviruses, herpesviruses, picornaviruses, and retroviruses. Preferred recombinant virus vaccines are those based on alphaviruses (such as Sindbis virus), raccoon poxviruses, species-specific herpesviruses and species-specific poxviruses. An example of methods to produce and use alphavirus recombinant virus vaccines are disclosed in U.S. Patent No. 5,766,602 to Xiong and Grieve, which is incorporated by reference herein in its entirety.
When administered to an animal, a recombinant virus vaccine of the present invention infects cells within the immunized animal and directs the production of a protective protein or RNA nucleic acid molecule that is capable of protecting the animal from flea infestation as disclosed herein. For example, a recombinant virus vaccine comprising a flea HMT andlor HNC nucleic acid molecule of the present invention is administered according to a protocol that results in the animal producing a sufficient immune response to protect itself from flea infestation. A
preferred single dose of a recombinant virus vaccine of the present invention is from about 1 x 104 to about 1 x 108 virus plaque forming units (pfu) per kilogram body weight of the animal.
Administration protocols are similar to those described herein for protein-based vaccines, with subcutaneous, intramuscular, intranasal, intraocular, conjunctival, and oral administration routes being preferred.
A recombinant cell vaccine of the present invention includes recombinant cells of the present invention that express at least one protein of the present invention.
Preferred recombinant cells for this embodiment include Sal>7zohella, E. coli, Listeria, Mycobacteriu~a, S. frugiperda, yeast, (including Sacclzaromyces cerevisiae and Pielzia pastoris), BHK, CV-l, myoblast GS, COS (e.g., COS-7), Vero, MDCK and CRFK
recombinant cells. Recombinant cell vaccines of the present invention can be administered in a variety of ways but have the advantage that they can be administered orally, preferably at doses ranging from about 10$ to about 1012 cells per kilogram body weight. Administration protocols are similar to those described herein for protein-based vaccines. Recombinant cell vaccines can comprise whole cells, cells stripped of cell walls or cell lysates.
The efficacy of a therapeutic composition of the present invention to protect an animal from flea infestation can be tested in a variety of ways including, but not limited to, detection of protective antibodies (using, for example, proteins or mimetopes of the present invention), detection of cellular immunity within the treated animal, or challenge of the treated animal with the fleas to determine whether the treated animal is resistant to infestation. Challenge studies can include direct administration of fleas to the treated animal. In one embodiment, therapeutic compositions can be tested in animal models such as mice. Such techniques are known to those skilled in the art.
One therapeutic composition of the present invention includes an inhibitor of flea HMT and/or HNC protein activity, i.e., a compound capable of substantially interfering with the function of a flea HMT and/or HNC protein susceptible to inhibition by an inhibitor of flea HMT and/or HNC protein activity. An inhibitor of HMT and/or HNC protein activity can be identified using flea HMT and/or HNC
proteins of the present invention. An inhibitor of HMT and/or HNC protein function can be identified using flea HMT and/or HNC proteins of the present invention.
A
preferred inhibitor of HMT and/or HNC protein function is a compound capable of substantially interfering with the function of a flea HMT and/or HNC protein and which does not substantially interfere with host animal proteins. As used herein, a compound that does not substantially inhibit or interfere with host animal proteins is one that, when administered to a host animal, the host animal shows no significant adverse effects attributable to the compound and which, when administered to an animal in an effective manner, is capable of protecting that animal from flea infestation.
One embodiment of the present invention is a method to identify a compound capable of inhibiting HMT and/or HNC protein activity of a flea. Such a method includes the steps of (a) contacting (e.g., combining, mixing) an isolated flea HMT
and/or HNC protein, preferably a C. fells HMT and/or HNC protein of the present invention, with a putative inhibitory compound under conditions in which, in the absence of the compound, the protein has HMT and/or HNC protein activity, and (b) determining if the putative inhibitory compound inhibits the activity. HMT
and/or HNC protein activity can be determined in a variety of ways known in the art, including but not limited to determining the ability of HMT and/or HNC protein to bind to or otherwise interact with a substrate. Such conditions under which a HMT
and/or HNC protein has HMT and/or HNC protein activity include conditions in which a HMT and/or HNC protein has a correct three-dimensionally folded structure under physiologic conditions, i.e. physiologic pH, physiologic ionic concentrations, and physiologic temperatures.
Putative inhibitory compounds to screen include antibodies (including fragments and mimetopes thereof), putative substrate analogs, and other, preferably small, organic or inorganic molecules. Methods to determine HMT and/or HNC
protein activity are known to those skilled in the art; see, for example, the Examples section of the present application. Methods to determine binding of a putative inhibitory compound to a HMT and/or HNC protein of the present invention are known to those of skill in the art and include, for example, determining changes in molecular mass using surface plasmon resonance (e.g., determining light scatter by an inhibitor of a HMT and/or HNC protein, before and after contacting the inhibitor or protein with a HMT and/or HNC protein or inhibitor, respectively) or screening for compounds that inhibit interaction between a HMT and/or HNC protein and a substrate.
A preferred method to identify a compound capable of inhibiting HMT and/or HNC protein activity includes contacting an isolated flea HMT and/or HNC
protein encoded by a nucleic acid molecule of Table I and/or Table II; (b) a protein comprising an at least 25 consecutive amino acid portion identical in sequence to a consecutive amino acid portion of a sequence as set forth in (a), wherein the protein has HMT
and/or HNC protein activity; (c) a protein comprising a fragment of a protein as set forth in (a), wherein the fragment has an activity selected from the group consisting of binding to a HMT andlor HNC molecule and hydrolyzing a HMT and/or HNC protein substrate; and (d) a protein encoded by an allelic variant of a nucleic acid molecule that encodes any protein of (a), (b), or (c), with a putative inhibitory compound under conditions in which, in the absence of the compound, the protein has HMT
and/or HNC protein activity; and determining if the putative inhibitory compound inhibits the activity.
Another embodiment of the present invention is an assay kit to identify an inhibitor of a flea HMT and/or HNC protein of the present invention. This kit comprises an isolated flea HMT and/or HNC protein of the present invention, and a means for determining inhibition of an activity of flea HMT andlor HNC
protein, where the means enables detection of inhibition. Detection of inhibition of flea HMT
and/or HNC protein identifies a putative inhibitor to be an inhibitor of flea HMT
andlor HNC protein. Means for determining inhibition of flea HMT and/or HNC
protein include an assay system that detects binding of a putative inhibitor to a flea HMT and/or HNC molecule, and an assay system that detects interference by a putative inhibitor of the ability of flea HMT and/or HNC protein to hydrolyze a substrate. Means and methods are described herein and are known to those skilled in the art.
The following examples are provided for the purposes of illustration and are not intended to limit the scope of the present invention. The following examples include a number of recombinant DNA and protein chemistry techniques known to those skilled in the art; see, for example, Sambrook et al., ibid.
Example 1 This Example describes the isolation of RNA from the hindgut and Malpighian tubules (HMT) of Ctenoceplzalides fells and the use of isolated RNA to construct subtracted and unsubtracted cDNA libraries.
Approximately 10,000 hindguts and Malpighian tubules were dissected from equal numbers of cat blood fed and unfed adult C. fells with a male to female ratio of 1 to 4, and total RNA was extracted using a guanidine isothiocyanate lysis buffer and the standard procedure described by Sambrook et al. Poly-A enriched mRNA was purified from total RNA above using a mRNA Purification Kit, available from Pharmacia Biotech, Piscataway, NJ, following the manufacturer's protocol. The same procedures were used to extract total RNA and isolate poly-A enriched mRNA from the dissected C. fells bodies following removal of HMT, referred to hereinafter as "non-HMT
mRNA".
Poly-A enriched mRNA was used to construct a cDNA library using subtractive hybridization and suppression PCR as follows. Subtractive hybridization and suppression PCR was conducted using a PCR-SelectTM cDNA Subtraction Kit, available from Clontech Laboratories, Inc., Palo Alto, CA according to the manufacturer's instructions. Briefly, this kit uses subtractive hybridizati~n and suppression PCR to specifically amplify cDNA sequences that are present in the tester cDNA and absent in the driver cDNA, thus enriching for tester-specific sequences.
The efficiency of the subtraction process can be assessed by semi-quantitative PCR
and by comparing the ethidium bromide staining patterns of the subtracted and unsubtracted samples on agarose gels as described in section V.D. of the manufacturer's protocol. For the semi-quantitative PCR, three genes with mRNAs known to be expressed outside of the HMT tissue were used to test for specific subtraction. These genes encoded putative actin, N-aminopeptidase, and serine protease proteins.
Subtractive hybridization and suppression PCR was conducted under the following conditions. Two micrograms (~ g) of HMT mRNA was used as the template for synthesis of the tester material and 2 ~ g of non-HMT mRNA was used as template for synthesis of the driver material in this reaction. The number of cycles used in the selective amplification steps was optimized using the manufacturer's protocols.
Optimization resulted in the use of 24 rather than the standard 27 cycles of primary PCR in combination with 15 cycles of secondary PCR rather than the standard 12 cycles.
The products from the suppressive PCR reaction were ligated into the pCR02.1 vector, available from Invitrogen, Carlsbad, CA, using an Original TA
Cloning0 Kit, available from Invitrogen. The ligation reaction was then used to transform INVaF' One ShotTM competent cells, available from Invitrogen, which were plated on Luria broth (LB) agar with 50 micrograms per milliliter (p g/ml) ampicillin, available from Sigma-Aldrich Co., St. Louis, MO, and 50 ~ g/m15-bromo-4-chloro-indoyl ~i-D-galactopyranoside (X-Gal), available from Fisher Biotech, Fair Lawn, NJ.
Transformed colonies were amplified and the DNA isolated using the standard alkaline lysis procedure described by Sambrook et al., ibid.
Automated cycle sequencing of DNA samples was performed using an ABI
PRISMTM Model 377, available from Perkins Elmer, with XL upgrade DNA
Sequencer, available from PE Applied Biosystems, Foster City, CA, after reactions were carried out using the PRISMTM Dye Terminator Cycle Sequencing Ready Reaction Kit or the PRISIV1TM dRhodamine Terminator Cycle Sequencing Ready Reaction Kit or the PRISMTM BigDyeTM Terminator Cycle sequencing Ready Reaction Kit, available from PE Applied Biosystems, following the manufacturer's protocol, hereinafter "standard sequencing methods". The resulting sequences are presented in Table II.
Sequence analysis was performed using the MacVectorTM sequence analysis software, available from International Biotechnologies Inc., New Haven, CT, and the SeqLab sequence analysis software, using default parameters. Each sequence read was trimmed of vector sequence at either end and submitted for a search through the National Center for Biotechnology Information (NCBI], National Library of Medicine, National Institute of Health, Baltimore, MD, using the BLAST network. This database includes SwissProt + PIR + SPupdate + GenPept + GPUpdate + PDB databases. The search was conducted using the xBLAST function, which compares the translated sequences in all 6 reading frames to the protein sequences contained in the database.
An unsubtracted HMT cDNA library was constructed as follows.
Approximately 10,000 HMT tissues were dissected from equal numbers of unfed and cat blood-fed adult C. felis with a male to female ratio of 1:4. Total RNA was extracted using a guanidine isothiocyanate lysis buffer and procedures described in Sambrook et al., followed by isolation using a mRNA purification kit, available from Pharmacia, according to the manufacturer's protocols. The library was constructed with 5 ~g of isolated mRNA using a ZAP-cDNA~ cDNA synthesis kit, and packaged using a ZAP-cDNAO Gigapack0 gold cloning kit, both available from Stratagene, La Jolla, CA. The resultant HMT library was amplified to a titer of about 5 x 109 plaque forming units per milliliter (pfu/ml). Single clone excisions were performed using the Ex-AssistTM helper phage, available from Stratagene, and used to create double stranded plasmid template for sequencing using the manufacturer's protocols with the following exceptions. Following incubation of the SOLR cells with the cleared phage lysate, the mixture was used to inoculate LB broth, and the mix was incubated overnight and then subjected to mini-prep plasmid preparation and sequencing as described for the subtracted HMT library above.

Example 2 This Example describes the isolation of RNA from the head and nerve cord (HNC) of Cteyzoceplaalides felis and the use of isolated RNA to construct subtracted and unsubtracted cDNA libraries.
Approximately 4,000 heads and attached nerve cords, including the terminal abdominal ganglia were dissected from equal numbers of cat blood-fed and unfed adult C. felis with a male to female ratio of 1 to 4, and total RNA was extracted using a guanidine isothiocyanate lysis buffer and the standard procedure described by Sambrook et al. Approximately 618 ~g of total RNA was recovered. Poly-A
enriched mRNA was purified from total RNA above using a mRNA Purification Kit, available from Pharmacia, following the manufacturer's protocol. Approximately 13 ~ g of mRNA was isolated. The same procedures were used to extract total RNA and isolate poly-A enriched mRNA from the dissected C. felis bodies following removal of HNC
tissues, referred to hereinafter as "non-HNC mRNA".
Suppression subtractive PCR was conducted as described in Example 1 using a PCR-SelectTM cDNA Subtraction kit, available from Clontech, under the following conditions. Two micrograms (~ g) of HNC mRNA was used as the template for synthesis of the tester material and 2 dug of non-HMT mRNA was used as template for synthesis of the driver material in this reaction. The number of cycles used in the selective amplification steps was optimized using the manufacturer's protocols.
Optimization resulted in the use of 24 rather than the standard 27 cycles of primary PCR in combination with either 12 or 15 cycles of secondary PCR.
cDNA pools from various PCR cycling combinations were ligated into the TA
vector using a TA cloning kit, available from Invitrogen. Aliquots of ligation reaction were transformed into Ultramax DH5«TM bacteria, available from Gibco-BRL, Gaithersburg, MD. Portions of the transformation mixes were used to inoculate LB
broth cultures containing 100 ~ g/ml of ampicillin. The overnight cultures were plated to generate discreet colonies which were used individually for overnight cultures for plasmid preps. Transformed colonies were amplified and the DNA isolated using the standard alkaline lysis procedure described by Sambrook et al., ibid.

Automated cycle sequencing of DNA samples was performed using the standard sequencing methods described in Example 1. The resulting sequences are presented in Table I. Sequence analysis was performed using the MacVectorTM
sequence analysis software, available from International Biotechnologies Inc., New Haven, CT, and the SeqLab sequence analysis software, using default parameters.
An unsubtracted cDNA library was constructed as follows. Approximately 6400 head and nerve cords were dissected from C. felis and poly-A RNA was isolated as described above. About seven p g of HNC poly-A RNA was used to construct a cDNA library using Stratagene's 7~ZAP-cDNA Synthesis Kit and protocol. The resultant HNC library was amplified to a titer of about 5 x 109 plaque forming units per milliliter (pfu/ml). Single clone excisions were performed using the Ex-Assist helper phage, available from Stratagene, and used to create double stranded plasmid template for sequencing using the manufacturer's protocols with the following exceptions.
Following incubation of the SOLR cells with the cleared phage lysate, the mixture was used to inoculate LB broth, and the mix was incubated overnight and then subjected to mini-prep plasmid preparation and sequencing as described for the subtracted library above.
While various embodiments of the present invention have been described in detail, it is apparent that modifications and adaptations of those embodiments will occur to those skilled in the art. It is to be expressly understood, however, that such modifications and adaptations are within the scope of the present invention, as set forth in the following claims:

FC-8-C2-PCT.ST25.txt SEQUENCE LISTING
<110> Brandt, Kevin S.
Games, Patrick J.
Stinchcomb, Dan T.
Wisnewski, Nancy <120> FLEA HEAD, NERVE CORD, HINDGUT AND MALPIGHIAN TUBULE NUCLEIC ACID
MOLECULES, PROTEINS AND USES THEREOF
<130> FC-8-C2-PCT
<140> not yet assigned <141> 2002-10-04 <150> 60/328,347 <151> 2001-10-10 <160> 350 <170> PatentIn version 3.1 <210> 1 <211> 549 <212> DNA
<213> Ctenocephalides felis <400> 1 cagattcagg acagtgttta ttaatatact gcggtttact gaaattttat aaatatgaaa 60 gtctttttca ttttcttttg tttatgcctg attattctta atgaactgga tgcgatgcga 120 gtaattccag gaggtcgata tggaaaacac aactacaaag gaaaacagcc tgagaaaaat 180 acaatatagg aacccggcag ctattaacta ttatgaccac aaagatggag ccaaaatagt 240 taagtcatca cacttcgaat tagattacat gttaggaaga aaaatcacat tcttctgcat 300 ggctcaagga ttaccgcgac cttcaataac ttggtttaag gatggagttg agttatttca 360 tcatcgattt ttccaagttc atgaatggcc atgggaaatg atactttgaa atctaaaatg 420 gaaatcgatc ctacgaccca aaaagatgca ggatattacg aatgctggca gacaatcaat 480 atgcaataga tagacgagga tttcgacaga ttacgactga ttgttattaa atattcatat 540 tgatatatg <210> 2 <211> 549 <212> DNA
<213> Ctenocephalides felis <400> 2 aaattttctt ttaataagaa aaatttatta ttaagtttat taattttaga atataaagct 60 ttaattatatttttaattttatatttttgttttaattatataatatttgagaattatttt120 tgtaatttttatattgtatttacagtatgtgaaggggttttaggcctttctattttagtt180 gcaataattccgtactcatggtaatgattattttaaaagatttaatataatacaatgtta240 FC-8-C2-PCT.ST25.txt aaatttttaataataatattttttataatccctattattttattaaaaaattgttattga300 ttggttcaaacaatattatttataatattatttatttatataattttaggaataaaaaga360 attataattgttaatattagattaaattttggttatgatattatatcttatagattgaat420 ttattaaggatttgaattattattttaatattaatagcatcaattttaatttataaaaat480 aattataatttaaattatttttattattattaattttttattattaatattatttttacc540 tttagtcat 549 <210> 3 <211> 485 <212> DNA
<213> Ctenocephalides felis <220>
<221> misc_feature <222> (129)..(129) <223> n = unknown at position 129 <220>
<221> misc_feature <222> (154)..(154) <223> n = unknown at position 154 <220>
<221> misc_feature <222> (296)..(296) <223> n = unknown at position 296 <220>
<221> misc_feature <222> (316)..(316) <223> n = unknown at position 316 <220>
<221> misc_feature <222> (475)..(475) <223> n = unknown at position 475 <400>

ggtcgtatgcacgcacctggaaagggtatctcccaatctgctttgccttaccgtcgtgga60 gtccccacttggttaaaattgaatggagaagatgtcaaagaacagatttttaaacttgga120 aagatggtnttactccatcacaaatctggtgttntcttaagggattctcatggggtcgcc180 caagttcgatttgtcagtggcaacaaaatcttgcgtatcatgaaagccatgggagtagcc240 cctgaattacctgaagatttatactatttgatcaagaaggctgtagctatccgcangcat300 ttggaaaggaaccgcnaggacaaagacagcaaattccgtttgattcttgagaatccagaa360 ttcacagacttgcccgttactacaaaacaaagagcatcttggacccaactggaaatacga420 FC-8-C2-PCT.ST25.txt atccagcact gatctgactt gtagctaaga tgttaatgaa tgtgaagtta tatantaaat 480 gtgaa <210>

<211>

<212>
DNA

<213>
Ctenocephalides felis <400>

ctaaaaatctagttcaaaatgcgtgaatgtatctcaatccatgttggccaagctggtgtc 60 caaattggaaacgcctgttgggagttgtactgtttggaacatggcattcagcctgatggc 120 caaatgccatctgacaaaactattggaggcggtgatgactctttcaacacctttttcagt 180 gagacaggggcaggcaagcatgtgccacgagctgtctttgttgatttggaaccaactgtt 240 gtagatgaggtccgcactggaacataccgtcaattattccacccagaacaactgattact 300 ggcaaggaagatgctgccaacaattatgcacgtggtcactacacaattggcaaagaaatc 360 gttgatgttgtattggcagaattcgaaattgctgatcaatgcactgtctgcaaggtttct 420 catttcactccttggagggggcaccggtcaggatcacctcctgtcatggacgtctgctgt 480 gctat <210> 5 <211> 500 <212> DNA
<213> Ctenocephalides felis <400>

acacatagcaaattaagtcttcgcttggatttacatgaaaattgccaaaaaaagaataca 60 ttcattataggaagtatttattcaagtattttttgatgactgttctagctgttcatcaat 120 tcatacttaaaatagttaccctttttaatttttacagtatttaaatttaatagttggtat 180 agtattttataaattttaatttgaagcattcctttataggtttggaaattatatttttac 240 aaaaaaaaataacccgtccaattgattttaaacaaaaccataaaaaactaggtgctatag 300 taaacagcatttgatttgatgtatacattgcattgaaatgagtggagaagcaagatgaaa 360 gcctatccattaatgacttaagactttttagaaatgttttatatatacatatgtatgcta 420 gatatttagatggtgtcttgtaattgaattagatttacaaggaaccaaatgttctacatt 480 aaaactaaatttatctctat 500 <210> 6 <211> 0 <212> DNA
<213> Ctenocephalides felis <220>
<221> misc_feature <222> (15) .(16) FC-8-C2-PCT.ST25.txt <223> n = unknown at positions 15 and 16 <220>
<221> misc_feature <222> (24) . (24) <223> n = unknown at position 24 <220>
<221> misc_feature <222> (18) .(18) <223> n = unknown at position 18 <220>
<221> misc_feature <222> (22) .(22) <223> n = unknown at position 22 <220>
<221> misc_feature <222> (25)..(25) <223> n = unknown at position 25 <220>
<221> misc_feature <222> (28) . (28) <223> n = unknown at position 28 <220>
<221> misc_feature <222> (31) . (31) <223> n = unknown at position 31 <220>
<221> misc_feature <222> (42) .(42) <223> n = unknown at position 42 <220>
<221> misc_feature <222> (44) .(45) <223> n = unknown at positions 44 and 45 <220>
<221> misc_feature <222> (53) .(55) <223> n = unknown at positions 53, 54 and 55 <220>
<221> misc_feature <222> (57) .(57) <223> n = unknown at position 57 FC-8-C2-PCT.ST25.txt <220>
<221> misc_feature <222> (8). (9) <223> n = unknown at positions 8 and 9 <400> 6 <210> 7 <211> 198 <212> DNA
<213> Ctenocephalides felis <220>
<221> misc_feature <222> (143)..(143) <223> n = unknown at position 143 <220>
<221> misc_feature <222> (167):.(167) <223> n = unknown at position 167 <400> 7 acatttagct ctatcggaat atctgaatat ctgaatatct gaatatctga atatccgaat 60 atccgaatat ccgaatatcc gaatatccgg atatccgaat atccgaatat ccgaatatcc 120 gaatatccga atatccgaat atncgaatat ccgaatatcc gaatatncga atatccgaat 180 atccgaatat ccgaatat 198 <210> 8 <211> 485 <212> DNA
<213> Ctenocephalides felis <220>
<221> misc_feature <222> (475)..(475) <223> n = unknown at position 475 <400>

gttttttgtttaacacttttgaataaaacaaaatatacaatggcggacgtagatgttcaa60 atgcaattcacaacaaccacgaccaaaacgcgtaaagtgaagaagtcgtccaagaggcgc120 gaatctagcgaccatggtgaagttactattacagagatggagaacggacaccaacagcaa180 ggtggcgagtacaccaaggccatgaacaaggactggcactctggtcacttctgctgctgg240 caatgcgacgagtcactgaccggccaacgctacgtactccgcgacgaacatccttactgc300 atcaagtgctacgagagcgtcttttccaacacctgcgaggaatgcagcaagatcattggc360 attgattccaaggacttatcttacaaagaaaagcattggcatgaggcatgtttcttgtgc420 agtaaatgccgcgttcttcgtcgtaacagttcggaagtaatggacaaattactgnggaac480 FC-8-C2-PCT. ST25 . tact tgtat <210>

<211>

<212>
DNA

<213>
Ctenocephalides fells <400>

aaggatggagtcactgtagcgaagggtattgatctcaaggacaaatttcagaatattggg60 gccaaattagtccaagatgtagctaataacaccaatgaagaagctggtgatggtactact120 actgctactgtgcttgccagagctattgcaaaggaaggctttgaaaaaatatctaaagga180 gcaaatcccattgaaatcagacgaggtgttatgctggctgttgatgcagtcaaagaatct240 ttgaaaggaatgtcgaagcctgttactaccccggaggaaattgcacaagttgctaccatt300 tctgccaatggagacaaagctattggaaaattgatctcagatgcaatgaagcgtgttgga360 aaggaaggtgtaataacggttaaagatggaaaaactttacatgatgaacttgaggtcatt420 gagggcatgaagtttgatagaggatcatttcaccttactttatgaattccagcaaaggtg480 ctaaagtagaattccaagatccttattttgtcagtgacaaaaaatactcagtcaagcata540 ttctgcttg 549 <210> 10 <211> 549 <212> DNA
<213> Ctenocephalides fells <220>
<221> misc_feature <222> (207)..(207) <223> n = unknown at position 207 <220>
<221> misc_feature <222> (513)..(513) <223> n = unknown at position 513 <220>
<221> misc_feature <222> (515)..(515) <223> n = unknown at position 515 <400> 10 acgccaggctatgcttcaagccaccaaggatgccaacaagaagggccccaacttcaccat60 caccaaaaaggacagcaacttcaacatgtcttctgcccaaattgaaagaaacaagaccaa120 ggaacaattggaagaggaaaagaaaatttctctgtccttccgtatcaagcctctggatat180 cgaatctcttagcgttgaaaaactcanacaaaaggccaatgaactttgggaatgcatcgt240 FC-8-C2-PCT.ST25.txt caagttggaaactgagaaatacgatctggaggaacgccaaaaacgccaggactacgatct 300 taaagaattgaaagaaaggcagaaacaacaactcagacacaaagcattgaagaaaggatt 360 ggatccagaagccctcacaggaaaatcccacccaaaatccaagtcgctccaaatatgaga 420 gacgtgtcgcacaagatcatatgatgataagaaaaaattatttgaaggcggttcgcacat 480 tgtctctgaagtaatgaaaagtctggaacagcntncaactattcagaaacgtagtaaact 540 agcttctaa 549 <210> 11 <211> 87 <212> DNA
<213> Ctenocephalides felis <400> 11 aatttccagc gcgctcagta ccaccacgtc gagacgatca accgatgcgc ctttcgaacc 60 ccagttagcg tactggttgg cgctgat 87 <210> 12 <211> 268 <212> DNA
<213> Ctenocephalides felis <220>
<221> misc_feature <222> (30) . (31) <223> n = unknown at positions 30 and 31 <400> 12 acggtttacaccctagtcgcgcctaaagannttttatttaaaaacaaattgtattacttt 60 taacaagtttctctacaaatttgagaaaaccatgttgtatttttaactactagtgtaata 120 tttttgttaaaatatattatttttgtctgtcccaaacgaatgattttcctgcggccctgc 180 ctgttgcagatattcctacacacgttattataagtcacgctgtttgcgcgccgtagcaat 240 taCgCCJCCCCgttcgcgccgttcgttgt 268 <210> 13 <211> 43 6 <212> DNA
<213> Ctenocephalides felis <400> 13 attttaaatt ttttataaaa ataatattta aaattataaa aaatatgaaa taatgattaa 60 attagtataa tataatttat tgataatatt aaattgttag gtttatttaa ggaattcggc 120 aaatataata ttcacctgtt taacaaaaac atgtcttttt gaaaataata taaagtctaa 180 tctgcccatt gaagatttaa aaggccgcag taatttgact gtgcaaaggt agcataatca 240 atagtttttt aattgaaagc tggaatgaat gattgaatga aatattaact gtcttaataa 300 FC-8-C2-PCT.ST25.txt aattgttata gaatttaatt tttatgttaa aaagcgtaaa tattattaaa ggacgagaag 360 accctttaaa gttttatata taaatataga ttattttaag aatattatta aatttattta 420 tattaatata tttatt 436 <210>

<211>

<212>
DNA

<213>
Ctenocephalides fells <400>

accttgaatagtaataattatctctttcaggacccccatttcgaatcaatattataatta60 aaccttctactttcagtgctgatgtatcctttatctatatatatgcaattagcaattgta120 taatttatatataaatgtgtatactttcgtaggttatggtagcgcatttccacattatcc180 aattttaagttcacaaaagtctctaatgtaagtcgtgtcttgtgtgtttcgtagagaatt240 tagtcggacaattgtaaaatgtcaaatagacttgtaaaagacaaagttatgtagtggttc300 taaagtagtgatattgtgtcgagt 324 <210> 15 <211> 391 <212> DNA
<213> Ctenocephalides fells <220>
<221> misc_feature <222> (385)..(385) <223> n = unknown at position 385 <400> 15 caagtctttacggatatcaacatcacatttcattatggagttgtaagtggtttcatggat60 tccacaggcttccattcccaagaatgatggctggaagagagcttctgggcacctgaacct120 ttcatttccaatggtgatgacttgtccgtcaggaagttcgtatgatttttctaaagatga180 agagctggcagcggtggccatttcttgttcgaagtccaaagctacatagcacaatttttc240 tttaatatcacggacaatttctctttcggcagtagtggtgaaggagtatccacgttcagt300 taagattttcattaaataatcagttaagtcacgggcagccaagtccaaacgcaagataac360 gtgagggaaggcgtatccttcatanattggt 391 <210> 16 <211> 440 <212> DNA
<213> Ctenocephalides fells <220>
<221> misc_feature <222> (100)..(100) <223> n = unknown at position 100 FC-8-C2-PCT.ST25.txt <220>
<221> misc_feature <222> (123)..(123) <223> n = unknown at position 123 <220>
<221> misc_feature <222> (132)..(132) <223> n = unknown at position 132 <220>
<221> misc_feature <222> (162)..(162) <223> n = unknown at position 162 <220>
<221> misc_feature <222> (178)..(178) <223> n = unknown at position 178 <220>
<221> misc_feature <222> (182)..(182) <223> n = unknown at position 182 <220>
<221> misc_feature <222> (184)..(184) <223> n = unknown at position 184 <220>
<221> misc_feature <222> (248)..(248) <223> n = unknown at position 248 <220>
<221> misc_feature <222> (286)..(286) <223> n = unknown at position 286 <220>
<221> misc_feature <222> (342)..(342) <223> n = unknown at position 342 <220>
<221> misc_feature <222> (403)..(403) <223> n = unknown at position 403 <400> 16 aatattattg tattatttag gcagcaggac attcaagagt tccatcattt atgtcatcta 60 FC-8-C2-PCT.

. tact ttaagtcatgtggtgccctgccatcaatggtgcatcctanactttgggctgttcccaaaa120 ccnctttaaacnttcccgaaaattcaccaaccaagggaccanggcccaattggggggnaa180 ancncaactatgtcatcaaaagtgatattgccattgtgtttaatgttcttttgcttcttg240 cggtcacnagggggttccttcaatgctttaataactaaagaggcanctgaaggtactact300 gaaattgttgcttgcctgttttggatggacaattgaacagtnattttcaaacccttccaa360 tccccggtagccttagcaatatcatcaccaactttctttgganacagaccaatggaccaa420 ttttgggggcaaaggatgat <210> 17 <211> 498 <212> DNA
<213> Ctenocephalides felis <2,20>
<221> misc_feature <222> (399)..(399) <223> n = unknown at position 399 <220>
<221> misc-feature <222> (448)..(448) <223> n = unknown at position 448 <220>
<221> misc-feature <222> (462)..(462) <223> n = unknown at position 462 <220>
<221> misc-feature <222> (486)..(486) <223> n = unknown at position 486 <400>

aatcaataagaatattggggccagttccatcaggaccaaagcaccagattttacgagctt60 cagttacatcataatcatattgttcaccaagataacgagcacgagctttgaattcatcac120 gggggttgacttttccactgtcgatgtcttctgcgaggccatcaggcataggttgtgcct180 tcatgaataatctgttgtgcttgtttggagatttagacaagcacatttggtctgattctt240 cagatacagtttctctgtaagatacaacagggtcagatttctttaatggaatgcaagcat300 ggtcttcttccaaatctttcaagcaaatttccaagtgcaattcaccggcaccagcaatga360 tgtgttcaccagattcttcaatgatacattggaccatangatctgatttaaccagacgtt420 tcaaaccttctaccaattttggcagatnagcaaggtttttangttcaacagcaacacgca480 caacangagaaactgaga 498 FC-8-C2-PCT.ST25.txt <210>

<211>

<212>
DNA

<213> felis Ctenocephalides <400>
18 gctcgtgtgttcggagccatgaagggtgctgttgatggtggcttaaatat 60 aaccactggt tccacactccactaagcgtttcccaggttatgatgctgaaagcaaaagcttcaatgctga 120 agtgcacagagctcacatctttggaaaacatgtagctgactacatgaaatctcttgaaga 180 ggaagatgaagatgcattcaaacgtcaatttagcaaatacatcagtcttggaattactgc 240 tgataatattgaagagctatacaagaaagcccatgcggccattagagctgatccttcatt 300 gaaacctgcagataaaatgaaggcagctgatgtcaaggtgaaacgttggaaccgggctaa 360 actttcactctctgaacgcaagaatcgcgttgcccaaaagaaagcttcatggcttcagaa 420 gctcaaagcagagaatgaggcataaatgacttctaaatatataaatgtttatattacttt 480 gaaataaaccggtaaaat 498 <210> 19 <211> 488 <212> DNA

<213> Ctenocephalides felis <400> 19 gccgtcagtg tgctggaattcggctttcgagcggccgcccgggcagtacaaatacatttc60 atcacttgca cacatccttaaattatgaagaacttagttgaacaaaccgaagcccatatc120 atcatcggac tcctctggttcagattcctt~cttcttttcctccttagcagcagcagctgg180 tgctgcagct ccaccggcagcagcaggggcagcggctgctggtccggatccaactcctga240 accgatgttg gtgatcaggtcttttacgtttactccttccaaagccttggcgaacaatcc300 tggccagtat ggctcaacatcgacagctgcagctttcaagatggtagagatcttctcacc360 agttacagca acatcatcgtcaaccaatatcagagcggagtaaacgcaagcgagttcagc420 ttttgtggcc atttttatatatatttgaaataacttttggtgcaagtcgccgaaatttgg480 tcttagct <210> 20 <211> 321 <212> DNA
<213> Ctenocephalides felis <400> 20 caagaaggtt ggaattactg gtaaatatgg aactcgttat ggagcttcct tacgtaaaat 60 ggtcaaaaag atggaaataa cccagcacgc caaatacact tgcacatttt gtggaaagga 120 tgccatgaaa cgcagttgtg ttggaatttg gtcttgcaaa agatgtaaaa gaactgtagc 180 FC-8-C2-PCT. ST25 . tact tggtggtgcc tgggtatata ccacaactgc tgctgcctct gtccgatctg ctgttaggag 240 gttgagagaa acaaaggaac agtaatttat attgatctta ataaataatt aataaaaaaa 300 aaaaaaaaaa aaaaaaaaaa a 321 <210>

<211>

<212>
DNA

<213>
Ctenocephalides fells <400>

tttttttttttttttttttttttttttttttagtttaatgtttattttaaatcataattt 60 acaaatatgattcaggaaattcaaatgttacctcacgtcctgtcaactttttgtaaacgg 120 aagtgaacgtatcaactttatgttcaattgtagtttgttgacttttgtccaaatgaactt 180 taatgagctgggatccatctagcttgacacggatacgtttaccaacaatttcagctggga 240 atactaagtcttctaaaatagcatcatacacagatgtcaaagttctgctacgtggacgct 300 tctgcttattcttagtgcgagttttacgtgttggttttggaaggattttacgctctccaa 360 caaatacaacatgattgccagaaaatttcttctccaactctcgt 404 <210>

<211>

<212>
DNA

<213>
Ctenocephalides fells <400>

gtgttgagtggtatcaaaacttggtcacccagtttatgag agaagtcttg ccacaacatt60 tccaaagcatttgtctggtgtagcaaaacatcgtgcccac tccatgggct ttcgtagact120 tcagatactgcttccattaaagacttggaagcactttgca cagctctaat acatcttatg180 tagttattaaattccttttgcagtctattagcactatttt gctgcctggt gaagttttgt240 aaatgctcatcaaaaatatcatctgcggt 269 <210> 23 <211> 216 <212> DNA
<213> Ctenocephalides fells <400> 23 ggcggctgat gccattagtg ggtttgtcca gaataagatt gtcgatggtg atgttatttt 60 aacatttagc tgctcatctc taattcaaca aatatttgaa gatacttcaa agaacggaat 120 tgattttcat gttgtggttg tggatggtcg cccatctcta gaaggtagag aaatgctacg 180 cagactcatt gaacgtggta tcaagtgttc ctatgt 216 <210> 24 <211> 500 FC-8-C2-pCT.ST25.txt <212> DNA
<213> Ctenocephalides felis <220>
<221> misc_feature <222> (230)..(230) <223> n = unknown at position 230 <220>
<221> misc_feature <222> (334)..(334) <223> n = unknown at position 334 <220>
<221> misc_feature <222> (394)..(394) <223> n = unknown at position 394 <220>
<221> misc_feature <222> (459)..(459) <223> n = unknown at position 459 <400>

ttttttttttttttttttttttttttaatttcataaagcttctatatttatatattttaa60 ataatcagaaattactatagttaatgacattttgtttgtgctccttacataaaatacaat120 tttatacattttaatttttatacatattttttatatttaattgcacagatcatttgtgta180 tttattatattaattgactactttatgataatttgaatcctaattagtcnattcaagatt240 gttttcctggtttaagccttacttagcaatctcacttttgcattcttcgccgggtggcgt300 gggtatgcatcgcaactccgttttatcaaagtgnaagccatttggacaatcataatgttt360 tatttgccatttgttgttaaccaagaaacattgntaatattttttacaatctcttcgatc420 ggaaaaaacgttttcaacctttgcacgcagttctgcccnagaaagattttgggtcaaacc480 attcgttgacgtcgtcctca <210> 25 <211> 146 <212> DNA
<213> Ctenocephalides felis <400> 25 ttttccatca actttgatta aacgctgcat aacaatcttc gtaacctcgg tattggtcaa 60 agcatatttc aaacggttac gtagaaaaat aactaaaggt aaggactcgc gaagtttgtg 120 aggtccagtg gatggccgag gtgcgt 146 <210> 26 <211> 498 <212> DNA

FC-8-C2-PCT. ST25 . tact <213> Ctenocephalides felis <220>
<221> misc_feature <222> (403)..(403) <223> n = unknown at position 403 <400>

ggccctggacaagatgcccttcgactcaccaccgaatatggagctgtctacgatcaaaat60 gccaccattgaagttcttaataaacagaagaggaaaactttgtgccacactgatggcgaa120 ggagttgaatgcgaaacagaagaagggagcatcgcacctcaaataatactatttgctgcc180 caattaatttctggagtcggtggatctttatactacactttaggtgtatcctatatggac240 gataacaccaaaaaatctaaaacaccagcactgataagtttttcttattttcttcgtatg300 ctcggacctgcaaccggttatgccttggctagcgtctgtctaaagttctacatttcgcca360 acgttgacgccaacaattgataataatgatcctagatggttangagcatggtggttaagt420 tgggtgatactaggatcaactttaatattttttgcaacgttgattggattgtttcctaaa480 attctgccaagagctgca 498 <210> 27 <211> 489 <212> DNA
<213> Ctenocephalides felis <220>
<221> misc_feature <222> (445)..(445) <223> n = unknown at position 445 <400>

27 aacaatatgtaaccgattccacactatttttaacagaattcaaaattcgtaattgtcaag 60 aaattatttcatctctacagaatgttttatgtgatcaagaagtcaagtatattccaaagc 120 ggaaatttgggttcaaaaataaaaagctcttattaaatgataaaattattggtaaggact 180 tggatacaaaaagtaaagatacgacagatttatgtctcaataagtgtaatgattggaatt 240 tatcagaatgtggatttcatttaaaggagaatgaaattctatatctttatgctgaacagg 300 ttaataaaaaggatattacattgagtagcatatataaatgcactgtaataattactggct 360 atcctaatacttcaaacattcaagacagcactgtgatttgtggtcctgtaactacatcaa 420 tatttgctgataaatgcattaatangtaagttagtcattgcttgccagcaattgagacta 480 cattcttct 489 <210> 28 <211> 582 <212> DNA
<213> Ctenocephalides felis FC-8-C2-PCT.ST25.txt <220>
<221> misc_feature <222> (422)..(422) <223> n = unknown at position 422 <220>
<221> misc_feature <222> (479)..(479) <223> n = unknown at position 479 <220>
<221> misc_feature <222> (520)..(520) <223> n = unknown at position 520 <400>

28 agctgccaattatagtaattagctactaaagctttccagttaactctggaactgctttaa 60 ataaatcagctactaaaccaaagtctgaaacttgaaatatgggtgcctctggatctttat 120 tgattgcaacaatagttttagaatctttcattccagctaagtgttgaattgctccagata 180 ttccaacagcaatatagagttcaggtgcaacaatcttccctgtctgaccgatttgcatat 240 catttgggcaaaatccagcatctaccgcggctctagaagcgcctactgcagctcctatct 300 tatccgctaagtcaaataataatttgaaattatcaccagactttaaaccacgtcctcctg 360 atacaatagtcttagcacttgataattcaggcctgtctgatttacttagttcttgggata 420 tnaatgtggtcaaatcgggattatagtcacctctgggaacatctttcacttcagctggng 480 aagaatttaatgcagctgggttcaaagctggtgccacgantagatatgactttagtggat 540 ctttcaccttcagtgtctgaatacgttaccggcataaattgt 582 <210>

29 <211>

<212>
DNA

<213> felis Ctenocephalides <220>
<221> misc_feature <222> (185)..(185) <223> n = unknown at position 185 <220>
<221> misc_feature <222> (258)..(258) <223> n = unknown at position 258 <220>
<221> misc_feature <222> (283)..(283) <223> n = unknown at position 283 FC-8-C2-PCT.ST25.txt <400>

taatgtcgtagaaggtgaaaaactgcgagttgtttgcacagttgtaggcaaacctacacc60 aacagtgtcatggaaagttattaatgagacttatgacgaatcatccgatcgaatcaaatt120 gttagatcatgacaatataccaaattctgctttggaaattgatttggctgaaaaaagcga180 tcgangagaatatacatgtattgctactaatcaaggcattggaattactgttattctaca240 accttggttcgagttcangataaaataactgctttgtggccanttttgggtatctgtgct300 gaagtaattacttt 314 <210> 30 <211> 133 <212> DNA
<213> Ctenocephalides felis <400> 30 ccgggcaggt acggcctctt gcaaggcttc gtggtgcatt tcaacggatt taacttcagt 60 ggtcaagttg gctggggcaa atacaacaac agtgcctggt ttcaagactc cagtttcaac 120 acggcctact ggt 133 <210> 31 <211> 485 <212> DNA
<213> Ctenocephalides felis <220>
<221> misc_feature <222> (2). (2) <223> n = unknown at position 2 <220>
<221> misc_feature <222> (216)..(216) <223> n = unknown at position 216 <220>
<221> misc_feature <222> (332)..(332) <223> n = unknown at position 332 <220>
<221> misc_feature <222> (406)..(406) <223> n = unknown at position 406 <220> ' <221> misc_feature <222> (463)..(463) <223> n = unknown at position 463 FC-8-C2-PCT.ST25.txt <220>
<221> misc_feature <222> (467)..(467) <223> n = unknown at position 467 <400>

angggatctgttctcacgcagattccgcacccgaggagtcatccctctgtctacttatat60 gaaagtgtataaagtgggagacatagtagacataaagggtaatggtgctgtgcagaaagg120 tatgccttataaagcctaccatggtaaaactggacgtgtttataatgtaacccctcatgc180 gcttggtgtaatagtaaacaaacgtgtccgtggtanaattattccaaagagaataaatat240 tcgtattgaacacgtaaagcattcaaaatgcagagaagattttttgaaacgtgtgaagga300 aaatgaacgtcttcgacatgaagccaaggaanagaaagttcatgttacattgaagagaca360 acctgctcaacctcggccagcaaatattgtcaaaggaactgatganccaatacgtttggc420 accaattccttacgaatttattgcataaatttgtaaactttanatanataattaagattt480 gtaaa <210>

<211>

<212>
DNA

<213> fells Ctenocephalides <400>

gtcgatagagatttggagaggttcaacaaagaagttgaaaggaaacttagcaaaaaagtg 60 agtgaagccgcaaaggaatgccttaaaggccaacaccaaaaagctctaggctacgcacaa 120 gaagcccgtgccaaggtcaaggcttgcagagatgacaaacgtgatgaattcaaccaagtt 180 cgccaaaatggcttggccgcctggagagaagccagggagaacgccgaagccctcagagca 240 caagccaaggagtgcgttgtgaccgcagccgatgtcgatgaagccagagtttgcttgaaa 300 ggggttctccaagctgcccacgaaggagcaaaagacttggccaaggcactcaaagaaacc 360 tggtcccaattgggcaacttagtccgtgaagtcggaaaatgcgcaaggggagccttgaaa 420 gaattagggacaacttagt 439 <210> 33 <211> 498 <212> DNA
<213> Ctenocephalides fells <220>
<221> misc_feature <222> (344)..(344) <223> n = unknown at position 344 <400> 33 attacacaga attcgtgcaa agaacactgt tttggcgact ggtggttatg gacgagcata 60 FC-8-C2-PCT.ST25.txt tttttcatgcacttcagcacatacctgcac tagccagagc120 aggtgatggc accggtatgg tggattaccagcacaagatttagaatttgtgcagttccatcctacaggtatttatggagc180 tggctgtttaattactgaaggatgcagaggtgaaggtggatttttggtaaattctcaagg240 agagagatttatggaacgatatgcacctgttgcaaaagatttggccagtagagatgtggt300 ttctagatcaatgacaattgaaatcaatgaaggtcgcggtgtangacctgagaaagatca360 cgttttattgcaattacatcacttaccagctgaacaattagcgagcagacttcctggtat420 ttccgagacagctatgatattttgtggtgttgatgttactcgtgagccaatccccgtttt480 accaacagtgcattataa <210> 34 <211> 427 <212> DNA
<213> Ctenocephalides felis <220>
<221> misc_feature <222> (403)..(403) <223> n = unknown at position 403 <400>

tgaaatgtgaagacgacgtcaacgaatggtttgacccaaaatctttctcgtgcagaactg 60 catgcaaaagtgaaaacgttttttccgatcgaagagattgtaaaaaatattatcaatgtt 120 tcttggttaacaacaaatggcaaataaaacattatgattgtccaaatggcttgcactttg 180 ataaaacggagttgcgatgcatacccacgccacccggcgaagaatgcaaaagtgagattg 240 ctaagtaaggcttaaaccaggaaaacaatcttgaatagactaattaggattcaaattatc 300 ataaagtagtcaattaatataataaatacacaaatgatctgtgcaattaaatataaaaaa 360 tatgtataaaaattaaaatgtataaaattgtattttatgtaangagcacaaacaaaatgt 420 cattaac <210>

<211>

<212>
DNA

<213>
Ctenocephalides felis <400>

cagtgcgggggcacctttgacactcgatttaataaagaaacgggtcacattagttttgca 60 actgtatgacaaaattgatccagcaaagttgaaattggagtcacactttatcaacgactt 120 aggtttagattccttagatcatgtagaagttattatggccatggaggacgagtttggttt 180 tgaaatcccagatggtgatgcagaaaaactgacccgacctgcagatattgttcgatacgt 240 agcagatagagaagatgtttatgaataaactaaataaatggttgatttgtaaaacatttt 300 agaataataatttcaatatctgcacaaaaattatgtgcagtttgattatgtctgaacctt 360 FC-8-C2-PCT.ST25.txt gt 362 <210> 36 <211> 462 <212> DNA
<213> Ctenocephalides fells <220>
<221> misc_feature <222> (445)..(445) <223> n = unknown at position 445 <220>
<221> misc_feature <222> (451)..(451) <223> n = unknown at position 451 <400>
36 ctatgctccatggtgtggacactgcaaagctttagccccagaatacgcaa60 ttgtcgaatt aagcagccaaacagttagaagatgaaaaatctgaaatcaaacttgccaaagttgatgcca120 ctgaagaaggcgatttggctgagaaatatggtgttcgaggatatccaactttaaaattct180 tccgtagcggaagtgaaattgattatacaggtggacgccaagctgatgacattattactt240 ggttgaaaaagaagactggaccaccagctaaagaactggaatctgttgatgacgccaaag300 catttgttgaggaaagccaagttgtcgttattggtttctttaaggatcgtgaatcagctg360 aagctaaggcattcttgtcaactgctaactctgttgatgattatccatttggtatcacga420 gcaatgaagaagttataacaattangatttnaatgtgggtca 462 <210> 37 <211> 0 <212> DNA
<213> Ctenocephalides fells <400> 37 <210> 38 <211> 429 <212> DNA
<213> Ctenocephalides fells <220>
<221> misc feature <222> (286)..(286) <223> n = unknown at position 286 <400> 38 gatcggttgc aatttgtcat tgcaccaagc ataaattgat ggggtccggt gcataggtta 60 taaaagaatt gggatttgtg catttcatga cggtgcaatc agcttctgac gatttctttt 120 FC-8-C2-PCT.ST25.txt tacacatatttttaaaatgatcatatacataagttaggtgggcattcgaaaacctgacct180 ttaacatctttgcagaataagtatcttgtgcaagtctttcgggatctggctgatatccct240 acaaaccgcgcatggtagtttctggccgtcttacagttttccccangttttgatgagcac300 atatcatctacacaaaatggagcatcggatttgagtctctgcaattgattgttgtattgg360 ggtttcttgtccagcgcacaacttcaaagtagaacaatcgtgacaaaatgttcccaactg420 cttattagt 429 <210> 39 <211> 498 <212> DNA
<213> Ctenocephalides felis <220>
<221> misc_feature <222> (426)..(426) <223> n = unknown at 426 <400>

tgtatgagagttttgcatcgccaggcgggatttattgctgaagttcaacaaagatgcttt60 catgtctcgccattaactgccatggccagcaaggtggccatgagcaaattcgacagtgat120 tcttatctgccttatggaaaattatcaagaaaatttaaaagttgttaaaaagcgtttgaa180 caggccattaactctttccgaaaaagtattatattctcatttagatgaacctgaaaaaca240 agaaattgaaagaggaactagctacttacgtttaagacccgatagagttgctatgcaaga300 tgctactgctcaaatggccatgttgcaatttatttcatcagggctgcctaaagttgccgt360 tcctagcacgattcattgtgatcatttaatcgaagcacaaatcggaggagatcaagatct420 tgctanagccaagacattaataaagaagtatacagtttcttatccagtgctagtgctaaa480 tatggggtgggcttttgg 498 <210>

<211>

<212>
DNA

<213> felis Ctenocephalides <400>

tgcctgacgaaattctcaaagaagtcgttcctgggtaatataagaaacgcagaacacttt 60 gtaggatttctgaaaagatttgtcgaatacatcaaaaccaggcttcgggtacagcatgtc 120 gtgcaggaatcaccagcaggttttctcaaagacatccagtcaaaagtatgcatcgaaaga 180 aagcctctgagattttgtgcagaaagactcgcttctctgcttcggactctggaagtgacg 240 gatatgactgattttggagccctggttttgatcacacatttagctacattggtttccacg 300 tatacacatggatttactattattgttgaaccattcgatgataagactccgaatgtttcg 360 aacccgattttgtatttcagttgtttagattcatcattagccatgaggccagttttctca 420 FC-8-C2-PCT.ST25.txt agatttcaat cagttgtcat cacatcagga actttgtcac ctttggatat gtatccaaag 480 attttggatt ttcaacct 498 <210> 41 <211> 177 <212> DNA
<213> Ctenocephalides fells <400> 41 aagggcaatt ttcggtttaa ggcatattgg aatgaggcat tagcgaaatc tacaattgaa 60 ctatcggtgt tgaacattcc catggcgact ccaggtcctt ggtaagtgtg gacggtgaag 120 ctcattggac ttcccgattc gggggtaaat gtcaattcta atttaccctt tcctggt 177 <210> 42 <211> 211 <212> DNA
<213> Ctenocephalides fells <400> 42 tgttccaata cctccaattt tgtagacatc ttgcaatggc agacggaggg gcttgtcggt 60 gggacgagct ggaggtaaga tggcatccaa agcttcaatc aagcatttgc cgtcagcctt 120 gccttcttta cgttcaatgt tccatccctt gaaccagttc atcttggtgg aaacttccaa 180 catgttgtct ccgtgccatc cagagatggg t 211 <210> 43 <211> 452 <212> DNA
<213> Ctenocephalides fells <400>

tgaatgtatgaatttggaagtctttagaattctgtattgggaaatcttcgcgataccatt 60 taatcgtaggtaaaggatcgccatcaaattccacttcaaaacgagcttgttcttgttcaa 120 aagctctgcaaggttgaattttctttgtgaaaaatggagctttagatggttttacaggac 180 cttgaacaactctttcaaatgttttgcccttgtgttcaacttctgtcgtttcggtcgcag 240 taatcttgcgggttatttctaaatctcccactgtttccttttgtgtttgcttttgtttgg 300 caacatgaacttcttttccatgcactgttgattctgaaggttcaggtgggtgacctggga 360 catgttgagaatctattatatattctgttgtatgtggcttttggatatattttgctttag 420 gaggagcatcaacagctgtctcagtagtttgt 452 <210> 44 <211> 498 <212> DNA
<213> Ctenocephalides fells <400> 44 FC-8-C2-PCT.ST25.txt tgcatctagttccagtcacatgttgatatt tgcattgcat 60 tggttcttgc aagcattgaa gtcccatttcatgaaaaagattatctaccatagctgggcttaaaagcgtggggcttgacc 120 atcgcggtggtgccaaatttaatacaactactactataggattctgatatgaaccatcgg 180 atttctgtttaccaccttgtatggtaaaatggcaatcttgatttggctttcctggtcttt 240 cataaaaatcacaatatatatgacctagagttccttcagtctcatgaacaatttttaatt.300 tatatatatcattagcccaagcttcaccaggtgccatttcttcaattacaaatttaatac 360 caaataaagattgcattaatatgtccaatccttccatacaaccaccaaggctaaaataag 420 gagcaaactcagatgctccaacttgtaaccattgcttcctgattttttgtgtaagtaagg 480 tgtatcccaagcagctaa <210> 45 <211> 498 <212> DNA
<213> Ctenocephalides felis <220>
<221> misc_feature <222> (488)..(488) <223> n = unknown at position 488 <400>

caaccgaggctcaagttaaagcactgtcaaaggaatgggctaaccgtgccacattaccag 60 ctcatgtagtcagtttattaaataatatgcctagcaaccttcaccctatgtcacagttat 120 catgtgccgtcacagcattaaacagtgagagcaaatttgcaaaagcctacagtgaaggag 180 tcaacaaagctaaatactgggagtatgtatttgaagacagtatgaacttaatt t gc 240 aaac tgcctgtagtagcagcaacaatataccgcaacacatacagggatagtaaaggaattagtg 300 ccattgatacaaacaaagattggtctgctaatttcacaaaaatgttgggttatgaggatg 360 aaaaattcactgaattaatgcgtctatacctcaccattcacagtgatcacgaaggtggca 420 atgtatcagcgcatacagttcatttagtaggatcagccctcagcgatcctacctctcatt 480 tgctgcangtatgaacgg <210> 46 <211> 500 <212> DNA
<213> Ctenocephalides felis <400> 46 tttttttttt tttttttttt ttttttttta ttattgaaat tcatttattg aatataatta 60 aaatttcaca tatttatatt cataatttta ctacatttct aagccttcat tataataccc 120 gttgcctgca gctccactag ttttacttgc attagaatta atattgtttg gataatcgtc 180 atttttaata cttgtatttg tattagttaa atttttggcc catggctgca cttcgccgga 240 FC-8-C2-PCT.ST25.txt tgcaaaaaaccaatatatcacagctcctaacaaatatattccacttgaaatgtaaaatac300 aatttgccacgatgtgagtaaatcatctccaacctttccattaacaatgaaaccagtcaa360 ttgagggctgattattcctggcaatgttgcaaaagtatttgacaatcccatcagaacgct420 agcatattgtggtgcgatgtccaaatgatttacactgaatccagaccaagcaaaagcgcc480 caaacccacagcgactgcag 500 <210>

<211>

<212>
DNA

<213> felis Ctenocephalides <400>
47 tatcaatgcttttgtctaagttcttgatgaacacattgcctacacctgac60 attgccttat ttacgcaaagaggatctctttgagaccacataatgcgaataggtctgcccttgagcatat120 cgaagttcatggtgtctatggctcgctcggcatcggcaggctgctggaaattaacgtagg180 cgtagcccaaggagcgcctcgtgatcatgtctcggcatacccttatcgacaatacgggcc240 ccgcagtcgaaaacttctcaaacaacatcgcctcggtgatgtcggaatgcagatctccaa300 cataaagggacgccatcgggtagttaggagcccctggattcatatcttacggcctttata360 tacttaaaccacgaacaaattaattattaataagaattgattaaaaatatctatatttcc420 aacgtggcgtatatctcctcaaaatggctcgtctgtcagtcaaaccacgttgaatgcgcg480 cactagtttcaacatttg 498 <210>

<211>

<212>
DNA

<213> felis Ctenocephalides <400>
48 gaacttgcgtggtgttagattcaacatttatgatgtcacacttcatgctg60 tttcagaaga atgctatccacagaggaggtggccaaatcattcccacaacacgtcgttgcttgtatgctt120 gcttaataacagctgcaccaagattgatggaacctgtatatcaatgtgaaattcagtgtc180 ctgaagttgctgttggtggtatctatggtgtgtggaactggtatagttatttgacgctcc240 ttctcgaaaacatacaaaacagccatgggaaaggaaaaggttcatattaacatcgtcgtc300 attggacacgtagattcaggtaaatccaccacaactggacacttgatctacaaatgcggc360 ggtatcgacaagcgt <210> 49 <211> 168 <212> DNA
<213> Ctenocephalides felis FC-8-C2-PCT.ST25.txt <400> 49 caaacggtgt gaactccttg cgcagccttt catcatcgat tgtgtcatca aggttcttga 60 catacaggtt aacaccttgg tagcgattca gacgttctat tttcagctgc tcaaacttac 120 gcttgagttc ttgttggcgt tcagctttct tttgagcacg gccaacgt 168 <210>

<211>

<212>
DNA

<213> felis Ctenocephalides <400>

attttcctttaactttaatgagaatggtcatatcagtaagatctttaccatcccatttat 60 caaatggttccaacaattgcaatcggtctgatttaggatcaacattaactttcacacttg 120 agccatctgcaggaggttgttcatatgtatcttgacctgcatcgaaacctttgcttggaa 180 gttcatcgccaaagggtgaatcaagtttgaattctttgccatcgcttcctttcagagtat 240 ctgttactggattaaaatctaagcggccagcaattgatagagctgtgactaattcaggac 300 ttgtgacaaaacagtgagtggcaggattagcatcattacggcctgtgaaatttctattat 360 atgaagtaacaattgtgtttttctctcctttcttcacatccttacgatcccactgaccga 420 tgcaaggtccacaggcattgctaaaacagtgcccccaaaattcacgggagaagtttgggc 480 aattccatctcgttcgat 4gg <210> 51 <211> 498 <212> DNA
<213> Ctenocephalides felis <400> 51 aatcaataag aatattgggg ccagttccat caggaccaaa gcaccagatt ttacgagctt 60 cagttacatc ataatcatac tgttcaccaa gataacgagc acgagctttg aattcatcac 120 gggggttgac ttttccactg tcgatgtctt ctgcgaggcc atcaggcata ggttgtgcct 180 tcatgaataa tctgttgtgc ttgtttggag atttagacaa gcacatttgg tctgattctt 240 cagatacagt ttccctgtaa gatacaacag ggtcagattt ctttaatgga atgcaagcat 300 ggtcttcttc caaatctttc aagcaaattt ccaagtgcaa ttcaccggca ccagcaataa 360 tgtgttcacc agattcttca atgatacatt ggaccatagg atctgatttg gccaggcgtt 420 tcaaaccttc taccaatttt ggcaagatca gcagggttct taggttcaac agcaacacgc 480 acaacaggag aaactgag <210> 52 <211> 310 <212> DNA
<213> Ctenocephalides felis FC-8-C2-PCT.ST25.txt <400>

cggcgcgacgatacgcgtggagtcttcgcgggggccggagtttatgatgacggttaccaa60 gatctgtaatggattttctccagtaagcaaatgaatgatttcaaaagcgtgcttgacaat120 acggatggccatcagttttttgccattgttacgtccgtgcatcatcagggagttggtcag180 acgttctacgatggggcactgggctttgcggaaacgcttggcagcgtatctaccagcaga240 gtggggtagatatttggcgttcttttctttgacggcgatgtaatcctgtagtgacatatc300 agagacttgt 310 <210>

<211>

<212>
DNA

<213> felis Ctenocephalides <400>

aatcacagcaggaagcaaatgacgacagggagtgccataaagatggatggaacttcttca60 acacagatattgttttcgacagaaatggagcagttgtggccagatatcgaaaattcaacc120 tttttagtgaaaaacaattcaataccactaaatttcccgaacattcgacatttgtcacag180 atttcaatgtaacgttcgggctgttcacctgtttcgacataatgttcaacgaaccagcct240 tggccatggtgagagaaggcgttagagactttgcattcccaactatgtggtttagcgaat300 tgccattcttaacagctcgtcaagt 325 <210> 54 <211> 623 <212> DNA
<213> Ctenocephalides felis <220>
<221> misc_feature <222> (581)..(581) <223> n = unknown at position 581 <400> 54 caactaataaaacaagatcggcttcttcagctcttgcaatagaattattcagtatataat60 ttgatctcaaatctgtgccggcaccttctgatggaaatgattgttcagtgtataaatttt120 ccgatcctattttattgaacaagtctttgagtgataccaatgcctctgcatcagcaagac180 caccagcgactgcagcaatctggcatggctccacaccttgcaaggctttacatacagcaa240 taagtgctgattcccaatcaacaacttctaattgtgatcctgatttccttaacatgggtg300 tgactaatctttgtcgtttaagaccatcaatagcaaatctagatttatcggataaccatt360 cttcattaatttcctcattagttcttggtaaaactcgtaaaacttccccagatcttgagc420 taacaacaatattgctccctatagcatctaaaatatcaatagaatcgatccggcgagttt480 cccaagagtgaaaagtaaacgctgtaggtttcgacgttaaagcaccaacaggacacaagt540 FC-8-C2-PCT.ST25.txt caataacatt accagataat tcaagtcata aaaaggtttt ncacatatgt gccaacttgc 600 atgtcgtttc cacgacctgt agt 623 <210> 55 <211> 527 <212> DNA
<213> Ctenocephalides felis <220>
<221> misc_feature <222> (528)..(528) <223> n = unknown at position 528 <220>
<221> misc_feature <222> (540)..(540) <223> n = unknown at position 540 <220>
<221> misc_feature <222> (556)..(556) <223> n = unknown at position 556 <220>
<221> misc_feature <222> (595)..(595) <223> n = unknown at position 595 <220>
<221> misc_feature <222> (615)..(615) <223> n = unknown at position 615 <220>
<221> misc_feature <222> (631)..(631) <223> n = unknown at position 631 <220>
<221> misc_feature <222> (658)..(658) <223> n = unknown at position 658 <220>
<221> misc_feature <222> (672)..(672) <223> n = unknown at position 672 <220>
<221> misc_feature <222> (688)..(688) <223> n = unknown at position 688 FC-8-C2-PCT.ST25.txt <220>
<221> misc_feature <222> (698)..(698) <223> n = unknown at position 698 <220>
<221> misc_feature <222> (702)..(702) <223> n = unknown at position 702 <220>
<221> misc_feature <222> (705)..(705) <223> n = unknown at position 705 <220>
<221> misc_feature <222> (707)..(707) <223> n = unknown at position 707 <220>
<221> misc_feature <222> (711)..(711) <223> n = unknown at position 711 <220>

<221> misc_feature <222> (744)..(744) <223> n = unknown position at 744 <400> 55 ttaattttaa taaaagtgcatgcgaaaatctagccgcaatgtctggaatgttgacgattt60 catcgcggac cattccgcgaaaagttttacgacttgcgacagatctcgtagaggcaaata120 attcaaaggt tctgaaagcagccttaagtactaaagcctcacacaaacaatgcgttctaa180 gactagCCaa accagaggtatataacatacctagaacaaaggtgttaatgaagtggcctc240 aacataatcg attagtgcactcttctgcagctttttatgcatcaaaagtagttaatgtac300 ctccatttgc agattctgtatcagaaggcgatgtcaagttggacaaaaaggtaggtgact360 atgtgtctgt cgatgaagtggttatggaaattgagactgacaaaacatccatgcctgttc420 cggcaccaga aggtggtgttattgaagaaatcttagttaaagatggtgacactgtcaaag480 ctggacaggc tttgttcaaactgaacacttcagctgcaccaccagca 527 <210> 56 <211> 0 <212> 17NA
<213> Ctenocephalides fells <220>

<221> misc feature <222> (5)..(5) <223> n = unknown at 5 <220>
<221> misc_feature <222> (37) . . (37) <223> n = unknown at 37 <220>
<221> misc_feature <222> (41) . . (41) <223> n = unknown at 41 <220>
<221> mist feature <222> (206)..(206) <223> n = unknown at 206 <220>
<221> misc feature <222> (248)..(248) <223> n = unknown at 248 <220>
<221> misc_feature <222> (279)..(279) <223> n = unknown at 279 <220>
<221> misc_feature <222> (298)..(298) <223> n = unknown at 298 <220>
<221> misc_feature <222> (306)..(306) <223> n = unknown at 306 FC-8-C2-PCT. ST25 . tact <220>
<221> misc_feature <222> (346)..(346) <223> n = unknown at 346 <220>
<221> misc_feature <222> (352)..(352) <223> n = unknown at 352 <220>
<221> misc feature <222> (356)..(356) <223> n = unknown at 356 FC-8-C2-PCT.ST25.txt <220>
<221> misc_feature <222> (364)..(364) <223> n = unknown at 364 <220>
<221> misc_feature <222> (371)..(371) <223> n = unknown at 371 <220>
<221> misc_feature <222> (386)..(386) <223> n = unknown at 386 <220>
<221> misc_feature <222> (401)..(401) <223> n = unknown at 401 <220>
<221> misc_feature <222> (409)..(409) <223> n = unknown at 409 <220>
<221> misc_feature <222> (431)..(431) <223> n = unknown at 431 <220>
<221> misc_feature <222> (448)..(448) <223> n = unknown at 448 <400> 56 <210> 57 <211> 498 <212> DNA
<213> Ctenocephalides felis <220>
<221> misc_feature <222> (496)..(496) <223> n = unknown at 496 <400> 57 acacatgatc taatcatcct tctttgaatc tgtctttcca ccacgtattc taccagtacg 60 tcttgcagca ataagaccaa tcttacgacc agcagatgtt cctctcttaa cagtagatgc 120 FC-8-C2-PCT.ST25.txt tttaccaatatgttgatggttaccacctccgtgaggatgctcaacagggttcatagcaac180 accacgaaccttaggccagcagttcctcttaaccttgtatttgtggtatgctctaccagc240 tttcaaaataggtttgtcaatacgtccaccaccagacacaataccaaccatagcacgatt300 gttagaaggcataacctttttagctccagatggaagcttgacacgagtctttttagtgtc360 cgggttgtgagcaataacagtagcgtagtttccagaagcacgggctagccgacctcggtc420 accagttttctcctctaagttgcatacaatggttccttctggcatagcaccaactggcat480 tacatttcctatttgnag <210> 58 <211> 190 <212> DNA
<213> Ctenocephalides felis <400> 58 acgtttgggc aatgggaacc tgattttcgc atcgtggaat tgtgtaactt gagggcgtct 60 acaattagca gctttcactt cttccacttt gattacttga atagagtgag cacgagccct 120 gtggcgtgca cccatgtcac gatagcactg agtgacggca cctccgacag ttaagtctct 180 gtattccctg <210> 59 <211> 393 <212> DNA
<213> Ctenocephalides felis <220>
<221> misc-feature <222> (61)..(61) <223> n = unknown at 61 <220>
<221> misc-feature <222> (69)..(69) <223> n = unknown at 69 <400> 59 tctccaatatcaaagcaggctacagacagaacaaggggatcacgagagctttcaagtaag 60 nggatcaanatacgcaataactcataattcttctcattgagacgtggggcattctctctc 120 cagaacttagcagatttgtgaacaggtgaccattccaatctgccggacttgatttctgta 180 gcatattcatcaaatgagctaaggtcttgaatggaagcttgtaatttttcggtcaaatat 240 tcaacatcagcaacgatatcctcatcatctgaacgtctttgttccaggatggataattgt 300 ttcaataccttgctttgcaccattgcaatgcaatgctccttggccacctgttgatcttcc 360 actttctcaattaaattcctataaacagccagt 393 FC-8-C2-PCT.ST25.txt <210> 60 <211> 307 <212> DNA
<213> Ctenocephalides fells <220>
<221> misc_feature <222> (62) .(62) <223> n = unknown at 62 <400>

tggcgacacctgactactctcacaccggtgagctacaaacgctcaacatgaagctaagaa60 anactctagagttatatgccaatgttgttcatgttaaaagtttgccaggagttcgctcta120 ggcataagaatattgactgtgtaatcattcgtgaacaaactgagggagagtatagtgctt180 tggagcacgaaagcgttacaggagttgtagaatgtttaaaaattataacagctaagaaat240 ctgaaaggattgccaaattcgcttttgactatgcaacaaagcacaatcgaaagaaggtga300 ctgctgt 307 <210> 61 <211> 229 <212> DNA
<213> Ctenocephalides fells <400> 61 gaaaggccgt gatctttttg ccatttttga tcaattggac acggacacac ttgcggatgg 60 cagagttggg ctgtttggct tctacgccaa ctttctccaa aacgattccc ttagcgtgag 120 aagcacctcc gaaaggattg gccttccacc tggttcctaa atgagccttt ttgtagtctt 180 tgtcggccca cctttggtca cgacgatggt taacatgttt tctagcagt 229 <210> 62 <211> 326 <212> DNA
<213> Ctenocephalides fells <220>
<221> misc_feature <222> (194)..(194) <223> n = unknown at 194 <220>
<221> mist-feature <222> (248)..(248) <223> n = unknown at 248 <220>
<221> misc-feature <222> (279)..(279) <223> n = unknown at 279 FC-8-C2-PCT.ST25.txt <220>
<221> misc_feature <222> (327)..(327) <223> n = unknown at 327 <220>
<221> misc_feature <222> (347)..(347) <223> n = unknown, at 347 <220>
<221> misc_feature <222> (362)..(362) <223> n = unknown at 362 <220>
<221> misc_feature <222> (369)..(369) ' <223> n = unknown at 369 <220>
<221> misc_feature <222> (374)..(374) <223> n = unknown at 374 <220>
<221> misc_feature <222> (381)..(381) <223> n = unknown at 381 <220>
<221> misc-feature <222> (397)..(397) <223> n = unknown at 397 <220>
<221> misc_feature <222> (400)..(400) <223> n = unknown at 400 <220>
<221> misc_feature <222> (426)..(426) <223> n = unknown at 426 <220>
<221> misc_feature <222> (432)..(432) <223> n = unknown at 432 <400> 62 atcactagct catcatgtta tagctgcttg gtttttaaaa tgcaggctgc ctttcaggag 60 FC-8-C2-PCT.ST25.txt agattttgtaaaatttatcaccacggtatgtgtgaaatataacaattgtgttaacttggt 120 agtaaacaaaatatattaatcaattcatgacgtatagggcctaaaatcaaattgcattgc 180 accatttgaggaangaaggatattaattaaaccagaggttacaactttgaatgaagaatc 240 atcttacngaaaaagaaagtctaggttaacagaaccagntaggtatttggaggttttcat 300 aagaaaaatgggtgaaatggtaaatg 326 <210> 63 <211> 525 <212> DNA
<213> Ctenocephalides felis <220>
<221> misc_feature <222> (5)..(5) <223> n = unknown at position 5 <220>
<221> misc_feature <222> (15) .(16) <223> n = unknown at positions 15 and 16 <220>
<221> misc_feature <222> (20) . (20) <223> n = unknown at position 20 <220>
<221> misc_feature <222> (24) . (24) <223> n = unknown at position 24 <220>
<221> misc_feature <222> (29) .(29) <223> n = unknown at position 29 <220>
<221> misc_feature <222> (31) . (31) <223> n = unknown at position 31 <220>
<221> misc_feature <222> (43) .(43) <223> n = unknown at position 43 <220>
<221> misc_feature <222> (48) .(48) <223> n = unknown at position 48 FC-8-C2-PCT.ST25.txt <220>
<221> misc_feature <222> (55) .(55) <223> n = unknown at position 55 <220>
<221> misc_feature <222> (127)..(127) <223> n = unknown at position 127 <400>

tgggnactattggtnngctntttntaaancnccccgcctttcnggtanatcctcncagtg 60 ggccagttagacataacgtggccacatatacctacagcatcccattcaatgttagataga 120 cctgatncatcggagtaagtagcctgcgaaagcaaatgctgtaaggcgtgtccaaatttg 180 ttgaataaaattgatacctcttttagagacagaagagaaggtatattgccctcaggagga 240 tttagacttaaaattaatgatgctaatggtgttgtatccacaattttacttttattgcgc 300 atactcaacatccatcctgaactcttttggaatatcttctcttcttctctagtataagga 360 tctaaataaaatccagccagtggcaattcagctgcagaatcatgtatatcaaaatacctt 420 acatctttatgccaagtattcatatttgacctttcgataattttaataccatacaacttt 480 tcacacaactgaaatagcccgttcaaaacCtttggaaatgggaag 525 <210> 64 <211> 164 <212> DNA
<213> Ctenocephalides fells <400> 64 gaaacaacag ctgctgggtt gtaaccgatc ttcttgatgt atgaggatac ttccttcttg 60 atttcctcga aacggttctc gctgtatggt ggctcagtgg agtccatctt gttgacacca 120 acgatcagtt gttttacacc gagggtgaaa gccaacaagg cgtg 164 <210> 65 <211> 490 <212> DNA
<213> Ctenocephalides fells <220>
<221> misc_feature <222> (4). (4) <223> n = unknown at position 4 <220>
<221> misc_feature <222> (12) .(12) <223> n = unknown at position 12 FC-8-C2-PCT.ST25.txt <220>
<221> misc_feature <222> (19) .(19) <223> n = unknown at position 19 <220>
<221> misc_feature <222> (22)..(22) <223> n = unknown at position 22 <220>
<221> misc_feature <222> (41) .(41) <223> n = unknown at position 41 <220>
<221> mist-feature <222> (74)..(74) <223> n = unknown at position 74 <220>
<221> misc_feature <222> (82)..(82) <223> n = unknown at position 82 <220>
<221> misc_feature <222> (107)..(107) <223> n = unknown at position 107 <220>
<221> misc_feature <222> (216)..(216) <223> n = unknown at position 216 <220>
<221> misc_feature <222> (252)..(252) <223> n = unknown at position 252 <220>
<221> misc_feature <222> (298)..(298) <223> n = unknown at position 298 <220>
<221> misc_feature <222> (450)..(450) <223> n = unknown at position 450 <400> 65 ctgncagtcc ancaccctnt gncaatttaa acattagctg naatcttcca gtggctccta 60 FC-8-C2-PCT.ST25.txt agtcaattccagancgatctanttcatctgtgtcaagtgaggattgnccagtttgtaaat120 ctagcctctcagtaacatttccaactttcataggtcgaccagccaattcaaatccattta180 actgctctaatgcttttttagcatcatcgtgattgngaaacgttaaaaagccatatccct240 tagaccgccggnttccggatccattatcaattgtatgctgtcaatttttccaaaaggntc300 aaatattcctcttaacatatcttcagttatattgaaatgtaacgaaccaacatataatct360 catgggaccagtatggcctttggtgctaaattgggcattgaattacccatgcgatttttt420 tcagcctgtgtgcgctgaactacaatcggnattcctaataacttttgcctggtagaccta480 aaggcaatgg <210> 66 <211> 97 <212> DNA
<213> Ctenocephalides fells <400> 66 ttctcatgat gggtcgttat gtagaactat tgaagatgtt ccttctggta acatttgcgg 60 tttggtcggt gttgatcagt tcttagtcaa gacaggt 97 <210> 67 <211> 533 <212> DNA
<213> Ctenocephalides fells <220>
<221> misc_feature <222> (30) .(30) <223> n = unknown at position 30 <220>
<221> misc_feature <222> (73) .(73) <223> n = unknown at position 73 <400> 67 ctggctaacacattatttagaaattactgnaattccgagaactcatgtcttaaaagaact60 tgctgaatactgntctgatgaaaaagaaaaagaacaattaaaattaatgagctcaactgc120 tccagaaggaaaacaattgtatcaagattgggttgttgaccagaaccgcaatattgtgca180 tattttggaagatctgccatcatgtcgtcctccaattgatcatctgtgtgaattgttacc240 tcgcttacaaccacgttattactcaatttcaagtagtgctaagctgtttcctaccacagt300 tcatatcactgctgttctagtgaagtataaaactccaactggtagaatcaacaaaggtgt360 tgcaacatcttggctggccaagttcaaccccagtcctgagaaacctcaagttgtgcctat420 atttattagaaaaagtcaattcagactgccaaccaggtctcagactccaattattatggg480 tggcctggccaaatctctttcttgtataaatcctctgaaaggagccaaacctg 533 FC-8-C2-PCT.ST25.txt <210>

<211>

<212>
DNA

<213>
Ctenocephalides fells <400>

tcgtggggtagttcttgggaaaatttcttggcttccctgtgatacatttcctgagcccgt60 gaacacgaacgtcaatggacctacagattttggcatcatacctaaagcgatttcatatcc120 agcatcccttatggattgtctggccatggaggaatttctgtaattgtgagccaatccaat180 atgcatgaaaggtgtatgatgacccaaagctaaaagacgtaaacctaatccgtgcaaaat240 attgatcataccggcaacaccagcatattttccaaatgctactactctttggcccttttc300 gtccatcaacttttcataatcgataagacgaatattcttttccataattgcatcaagtaa360 aggcatattagattcttgggctttgataacatgtgaaaacatgcagtaggttttattggg420 aattaattgttctacaggt 439 <210>

<211>

<212>
DNA

<213>
Ctenocephalides fells <400>

ccacaggaaaggaggtagctatcaaaattattgacaaaac acagctcaatcctggttcgc60 ttcaaaaacttttccgagaggtgcgcatcatgaaaatgtt ggatcacccaaatattgtaa120 aactgtttcaagtgattgagacagaaaaaactttatatct agtaatggaatatgcatcag180 gtggtgaagtgtttgattatcttgttcttcatggtaggat gaaagaaaaggaagctagag240 ccaaatttaggcaaattgttagcgctgt 268 <210> 70 <211> 145 <212> DNA
<213> Ctenocephalides fells <220>
<221> misc_feature <222> (49) .(49) <223> n = unknown at position 49 <220>
<221> misc_feature <222> (71) .(71) <223> n = unknown at position 71 <400> 70 ctctcgtcgc taactctcaa ttcctgggtg acttgctcgt agtctccgnt caaagtcgat 60 aattcctgct ngattttgct ctttgagctt gccaaattga cgttgattac cgtcaactcg 120 FC-8-C2-PCT.ST25.txt ttgatgcggg cgacgctctc ctcgt 145 <210> 71 <211> 327 <212> DNA
<213> Ctenocephalides felis <220>
<221> misc_feature <222> (207)..(207) <223> n = unknown at position 207 <220>
<221> misc_feature <222> (227)..(227) <223> n = unknown at position 227 <220>
<221> misc_feature <222> (304)..(304) <223> n = unknown at position 304 <400>

gagtgcacagaaaattctctaccttgagaaagtttatggaaaaattagagaattgtaact 60 atgctgttgatcttggaaaacagatcaagttctcactagttggtattgctggacaggaca 120 tcaagtgaaggaaatgctactttaactttagcgcccatatggcagcttgatgagagctta 180 tactttatctgtgttgacacgtttggnaaacactggaagtccaatanttgaaagagaaat 240 tgttcagtgggtgaataccaagctagcaggtgccaataaa.acaacatctttgagtagctt 300 tccnaggacaaaacaatttcagatgct 327 <210>

<211>

<212>
DNA

<213> felis Ctenocephalides <400>

cattgtggatgaggcaaatcccatataccatgatgaagtttgcttgtttcgaaagaacag60 ttgaattgttatacactcatgtggttcccaaacccagagcagaatgcactaaaggtgaac120 aattggttgtcacctttgctgctggttacattgccggtgtattctgtgcagtagtttctc180 atcctgcagacacagttgtttccaagctaaatcaagacaaaggagcaacagccattgacg240 ctgcaaaaaacttggctttgctggtttatggaagggattaggacctaggatcatcatgat300 tggt <210> 73 <211> 232 <212> DNA

FC-8-C2-PCT.ST25.txt <213> Ctenocephalides felis <400> 73 caaagtgatt taccagagaa gtttgtgaaa gacttgggtg tcctcaacgc tttatcagac 60 aaagtcttgg aattaacccc cgatgaacgc cctacctgtt cttcttccgc ttatcaacaa 120 atttcaaatc ccccgaagac accgacaaag ccaaaacaga gctcaccaaa gccatcaaaa 180 ccttgagcga ggcttttgat aaaacctaca aaggcaaagt gctgttcaca gt 232 <210> 74 <211> 339 <212> DNA
<213> Ctenocephalides felis <220>
<221> misc_feature <222> (108)..(108) <223> n = unknown at position 108 <220>
<221> misc_feature <222> (284)..(284) <223> n = unknown at position 284 <400> 74 gtaacccgattctacacgagttggttttaaaactaagctcggatgacgtttatgttaatc 60 aataagtatgcagaagaatatgttttcttaaaacattggacaggaacncacgaagaagat 120 caagtccaaataaagaaacgtcgtgaagtcttggagcgtttttgcaaacttattgaattt 180 ggtatcataccagttaccagcgcgtcctttgttttgatgtattatgtgaagtgtgaaaaa 240 tgctatggagatatcataaagaaaatggttgccacgatgtgcancctcagcaggacaaat 300 tgcaccatggtcatgttcctgagtttggcgaaaatttat 339 <210> 75 <211> 339 <212> DNA
<213> Ctenocephalides felis <220>
<221> misc_feature <222> (108)..(108) <223> n = unknown at position 108 <220>
<221> misc_feature <222> (284)..(284) <223> n = unknown at position 284 <400> 75 gtaacccgat tctacacgag ttggttttaa aactaagctc ggatgacgtt tatgttaatc 60 FC-8-C2-PCT.ST25.txt aataagtatgcagaagaatatgttttcttaaaacattggacaggaacncacgaagaagat120 caagtccaaataaagaaacgtcgtgaagtcttggagogtttttgcaaacttattgaattt180 ggtatcataccagttaccagcgcgtcctttgttttgatgtattatgtgaagtgtgaaaaa240 tgctatggagatatcataaagaaaatggttgccacgatgtgcancctcagcaggacaaat300 tgcaccatggtcatgttcctgagtttggcgaaaatttat 339 <210> 76 <211> 188 <212> DNA
<213> Ctenocephalides felis <220>
<221> misc_feature <222> (39) .(39) <223> n = unknown at position 39 <220>
<221> misc_feature <222> (61) .(61) <223> n = unknown at position 61 <220>
<221> misc_feature <222> (182)..(182) <223> n = unknown at position 182 <400> 76 cccagaagta tctgctttgg agcacgattt attgaagtng atccagaaac taaagaaatg 60 ntgagcatgc tagatttcaa gaacatcagt ggtctgcaat taactcaacc ccaaacattc 120 aatgctaggc gttaatatga aaatgtcagt tatattctta taaataaata tatataactg 180 gnaaataa 1gg <210> 77 <211> 468 <212> DNA
<213> Ctenocephalides felis <220>
<221> misc_feature <222> (436)..(436) <223> n = unknown at position 436 <220>
<221> misc_feature <222> (442)..(442) <223> n = unknown at position 442 <400> 77 ccacagaatt aaggtggttt caaggcccaa gacttgcaga acactttcat gatcaaagaa 60 FC-8-C2-PCT.ST25.txt ttgtcccagtatatttagatatggaggttgaacatccactattcactgaaactattgata120 tgtgcttatctgtttatccacctgatgccggacctccacatgtaaagcttcagttagaat180 ggtctatgaaagaatatgaagaaattattcaacatccaggggatgttttagaagatcatt240 ttagtgtgtctgaagtgctgccaaaagtgaaaatgcaaatcctctaactttagagcagtg300 cttagaacattataccagagcagagactctctctgatgaagatgcctggaggtgtccaca360 ttgcaaaagtatatgccggttgttaagactttgggcttgtggcattacctgatatactag420 taatacattttaaganggtcangcagcaccaaattaaggcaatagaag 468 <210> 78 <211> 382 <212> DNA
<213> Ctenocephalides felis <220>
<221> misc_feature <222> (13)..(13) <223> n = unknown at position 13 <220>
<221> misc_feature <222> (67)..(67) <223> n = unknown at position 67 <400>

ttaagtctggatncacccaataacaatggtgggtggaaacgcccatagaattcatccaat 60 tccgggnagtttttttcttcgcggggtctcttcacaggtctctaatccctctatttcgcc 120 ttctggacgatctaatcctggatgggtgttgaacaagttggctacgaacgctaaattcaa 180 cttataaacgccattaacaacatctgctgctgttacaaagcttctgcatttgagtttagc 240 agcttgttgcagcatgatctcagctctctttgtcatatcttgttccatgagagcttcgtt 300 cgtaacttgagcttctggaggtgcaatttgtttcaacagaatagtataaacctctgaatc 360 cgaaatgtcgacttggaagtto 382 <210> 79 <211> 141 <212> DNA
<213> Ctenocephalides felis <220>
<221> misc_feature <222> (55)..(55) <223> n = unknown at position 55 <400> 79 gtttgtgctt gcacatataa atattaaaaa tgtctaaaag aggacctggt ggttnagccg 60 FC-8-C2-PCT.ST25.txt gagctaaatt ccgcatctcc ttgggtctgc ctgtcggagc tgttattaat tgtgccgata 120 acactggggc caagaacttg t 141 <210> 80 <211> 247 <212> DNA
<213> Ctenocephalides felis <400>

gttgacccataatcggtttgtagttaacacatcttctacttcatctaaacgctcagcaaa 60 ctttgaagcaaattcgtaaatatcatccatcaatccaattgggcatatcctgtgacacac 120 caccaggacgaatataagcagcatgcattctagcgcctgatactctttcatagaattcca 180 tcattttttctctttcctcaaaaagccagaaaaagggtgtcaaagctcctacatccaatg 240 catgtgt <210> 81 <211> 498 <212> DNA
<213> Ctenocephalides felis <220>
<221> misc_feature <222> (11) .(11) <223> n = unknown at position 11 <220>
<221> misc_feature <222> (28) .(28) <223> n = unknown at position 28 <220>
<221> misc_feature <222> (31) .(31) <223> n = unknown at position 31 <220>
<221> misc_feature <222> (72) .(72) <223> n = unknown at position 72 <220>
<221> misc_feature <222> (74) .(74) <223> n = unknown at position 74 <220>
<221> misc_feature <222> (105)..(105) <223> n = unknown at position 105 FC-8-C2-PCT.ST25.txt <220>
<221> misc_feature <222> (128)..(128) <223> n = unknown at position 128 <220>
<221> misc_feature <222> (170)..(170) <223> n = unknown at position 170 <220>
<221> misc_feature <222> (424)..(424) <223> n = unknown at position 424 <220>
<221> misc_feature <222> (457)..(457) <223> n = unknown at position 457 <220>
<221> misc_feature <222> (490)..(490) <223> n = unknown at position 490 <400>

aatttctccgntaattggtggagcctgntgntcttcttgagtagagatttcagtaagaga60 attacagtttgngntgccggaagccaattgcgcttttaataaagnattttggtcttttaa120 ttgattgntacttatcactacttcctctagctgactctggattgcattgntttgattgga180 taactgctgagcttgcatgcgcaattgcatcatctgcatatcttgctctcgaataatatt240 tttatatttgcacaataaagcagactcttgttcagataacgacatttcagataaattcaa300 attattggttccatctccaaatgatgttattgtcttgacaataagtgcctctagagcttt360 gaatagttcacaaaattcataatcaagtaacagctctctatacttagtagcttttatagc420 aggntgctttgcagctaaactatacatctcgtgccgngagaattttgatagctttgcaac480 aaacgtctcnctccaatt <210> 82 <211> 353 <212> DNA
<213> Ctenocephalides felis <400> 82 agtatacttt gacaaaatat aaatgccaat atgcagtcga agaataggcg ttcttgctgt 60 cttgcacaaa tctccagaca tattgtggga tgttcaaaat tactttttag aagcagcacc 120 aactttagat tttttcgttc ctcggacttt cttcattctg ttcttacgtt ctttacgttg 180 tttacgtgtc tgttgcttct tttcatagag accgtgcctt cctaacctgt gtttaggttc 240 FC-8-C2-PCT.ST25.txt aaatttcttg gcatagtcta atgagtcgta gattaaagca aatccagttg atttgccacc 300 accaaagttt gtgcggaagc cgaatacaaa tactacatct gatgtaactt tgt 353 <210> 83 <211> 498 <212> DNA
<213> Ctenocephalides fells <220>
<221> misc_feature <222> (205)..(205) <223> n = unknown at position 205 <400>

ccaaatattgcagttcctacacgaacaatattgctgcccatttcaatcgcttgttcaaag60 tcgtcagacattcccatagataaattaacttcttttggttctaaatttaattccttgcat120 aattgctcacgacattgttttaaagtgagaaaatctgggttgggtcctaaacttgtatca180 tagccatattttccaatagtcattnaaccatcaacaagtaggtttgggcaattttctttc240 acatatttgtatagagttgttgcctcatttgggtgaacaccatgtttttctatttcacca300 cttgtatttatttgaatcataactcttaatttatcaccatgaattttattagctgaatct360 aattttgaccaagcattctgcacactgcggctaatttaaatgagtgaattgtttcgacca420 tatatatcccaggtatgttaaccaccttattaattttatttctttgaagatgacctataa480 aatgccatttatttcttt 498 <210> 84 <211> 268 <212> DNA
<213> Ctenocephalides fells <220>
<221> misc_feature <222> (4). (4) <223> n = unknown at position 4 <220>
<221> misc_feature <222> (35) .(35) <223> n = unknown at position 35 <220>
<221> misc_feature <222> (156)..(156) <223> n = unknown at position 156 <220>
<221> misc_feature <222> (181)..(181) FC-8-C2-PCT.ST25.txt <223> n = unknown at position 181 <220>
<221> misc_feature <222> (233)..(233) <223> n = unknown at position 233 <400> 84 ttgnttccactttggccccaacagtgaggaaaggngtgtattatttaagtatagcagctg 60 caggatttttggtattcataattgcattaacaattatacgtttaataatattctgtataa 120 tttggatcataacacttggtaaacatcatttatggntatttccaaatttaacaaaagaag 180 ntggggtctttgcatcattttggcctctatataagtatgaatataaaggaggngaagcat 240 tagaaaatactagcaaaaaaaaaaaaaa 268 <210> 85 <211> 223 <212> DNA
<213> Ctenocephalides felis <220>
<221> misc_feature <222> (19) .(19) <223> n = unknown at position 19 <400> 85 aggttcaaca ccaactgtng agtatccaac caatcttgcc aaaggtttta agccatgctg 60 tttgcaggat tcttctgatg Ctaaaacaat cgctgctgct ccatccgata ttccggaagc 120 agatccagct gtgacaacgc catctttctt gaaaattgta ggtaatttgg ataaactttc 180 tagagttgtt tgtggtcttg gatgttcatc tacacagaat tgt 223 <210>

<211>

<212>
DNA

<213> felis Ctenocephalides <400>

attgccttctttatctagtcgggttttcattcttgcgataaaatcgttggcaatttcttc 60 aatccatccaacatattgccttgtaatcttatgctgcatcatgggctgatttactactga 120 ccgaattttttgccattcttccccttgtgagtgaattagaccataaacagatttgaaaac 180 ttctggtcttttttctctcaagtaatattctcgtgattcaaaagctggtctatcaggcca 240 aggcccttcagt 252 <210> 87 <211> 235 <212> DNA
<213> Ctenocephalides felis FC-8-C2-PCT.ST25~.txt <400> 87 aatccaggga gaccatcaga cttgaagatt ttggtcaagc agttgcccaa tccagagaat 60 tcacgttgac cagcgccttt acctacatca gcggccaatc tggtacgggc gaaatcaagt 120 gggtagacga agcacaatga tgtggctcca gcagcaccac cagatgccaa gtttcctgcg 180 aagtaacgcc agaattgggt cttcttgtca actccaccca agaaaacttg cttgt 235 <210> 88 <211> 498 <212> DNA
<213> Ctenocephalides felis <220>
<221> misc_feature <222> (59)..(59) <223> n = unknown at position 59 <220>
<221> misc_feature <222> (365)..(365) <223> n = unknown at position 365 <220>
<221> misc_feature <222> (404)..(404) <223> n = unknown at position 404 <220>
<221> misc_feature <222> (456)..(456) <223> n = unknown at position 456 <400>

ataaagtatccgttcaatggtaacgtttcagcagcaaaccaataattagtaacatctana60 aaaggagctgataatgacaaagctgctaaaaaacaggtatgtcttaaagcaactcttcta120 cataatgcaggatcagtaactgccatcattctatacccagctctactgtaatccggtctt180 aaattccaagataatgcattaaaatgtggaaattgccacgtatataaaagacctcctaag240 ataaatgctcctgcacttaaattaccggaacaagcagcccaacccattaggggtgggatt300 gctccgacgattgatccaacccaagtattcagaatacttattcttttcattggagtatat360 acggnggtatataatattaaattagctgctcctaatcctgctgntaaaccatttacacca420 aaatatacatacctatcccgtagatgcagcaaccgnagcaaaacctacagcatgtatgga480 cttaaaatcccctgacta <210> 89 <211> 293 <212> DNA

FC-8-C2-PCT.ST25.txt <213> Ctenocephalides felis <220>
<221> misc_feature <222> (24) . (24) <223> n = unknown at position 24 <220>
<221> misc_feature <222> (50)..(50) <223> n = unknown at position 50 <220>
<221> mist feature <222> (92)..(92) <223> n = unknown at position 92 <220>
<221> misc_feature <222> (282)..(282) <223> n = unknown at position 282 <400> 89 tttttttttttttttttttttttngcacattatattaatataatcacttnggccttttta 60 gacttgatcttcctcatgtagaattccaattnttttccttctaagatataaccatctgca 120 cgtccacattgtccaggacgagaggaaacagcagctaatagacgaccagtagcaaactgt 180 tcttccagagcttgctcaactttagcaacacgttgtcttgttgcatattttttagccacc 240 ttcttactgcgttttttattcataactgcttcttcttcaggngtgagcttagt 293 <210> 90 <211> 498 <212> DNA
<213> Ctenocephalides felis <220>
<221> misc_feature <222> (28) . . (28) <223> n = unknown at position 28 <220>
<221> misc_feature <222> (66)..(66) <223> n = unknown at position 66 <220>
<221> misc_feature <222> (73) .(73) <223> n = unknown at position 73 <400> 90 cattcatgca aatggggcat catttttntt tatttgtatt tacattcata ttggacgagg 60 FC-8-C2-PCT.ST25.txt aatggnattattntagttacaaattacatgaaacttgatttattgggttattaattttat120 ttttagtaataggaactgcttttataggatatgtattaccttgaggacaaatatcatttt180 gaggagcaactgttattacaaacttaatttctgctattctatatattggaaattcaattg240 ttcaatgattatgaggaggatttgcagttgataatgctactttaactcgattctttatga300 ttcattttttattaccatttattatttctgcttttgtaataattcatttgctttttcttc360 atcaaactggatctaataatcctttaggaattaatagtaatatttacaaaattccttttc420 atccttatttctctctaaaagatttattaggatttatttttttatttacaatattaatta480 tattaactcttattaatc 498 <210> 91 <211> 339 <212> DNA
<213> Ctenocephalides felis <220>
<221> misc feature <222> (46)~.. (46) <223> n = unknown at position 46 <220>
<221> misc_feature <222> (208)..(208) <223> n = unknown at position 208 <220>
<221> misc_feature <222> (223)..(223) <223> n = unknown at position 223 <220>
<221> misc_feature <222> (234)..(234) <223> n = unknown at position 234 <220>
<221> misc_feature <222> (289)..(289) <223> n = unknown at position 289 <400> 91 gggatatattataagatctttattttataataaattatataatcgntatttacttgaaag 60 acaaaatattgaaattatttgaacaattctacctgcatttatattaatttttattgcttt 120 accttctcttcgacttttatatttattagatgattcaaataggcctttaatttctttaaa 180 agcaattggtcatcaatgatattgaagntatgaatatactgantttaataatanttcttt 240 tgattcatatataattccatctaatgaattaaatttaaattcatttcgnttattagatgg 300 FC-8-C2-PCT.ST25.txt tgataatcga attatcttac ctattaattc acaaattcg 339 <210> 92 <211> 234 <212> DNA
<213> Ctenocephalides felis <220>
<221> misc-feature <222> (49)..(49) <223> n = unknown at position 49 <220>
<221> misc_feature <222> (59) .(59) <223> n = unknown at position 59 <220>
<221> misc_feature <222> (61) .(61) <223> n = unknown at position 61 <220>
<221> misc_feature <222> (68) . (68) <223> n = unknown at position 68 <400> 92 cagtgcaaca gcatttaacc atgcttatgc tgataccggt ctattctgna ttcatgccng 60 ngcacctnca acacatatac ggggggtaac agaagtgctc tgcagagaac tagttgccat 120 gtctacagtt ccaggggctc aagaactaag gagagcaaaa acacaacttc aatcaatgtt 180 attgatgaat ttggaagctc gtcctgtggt atttgaagat ataggtagac aagt 234 <210> 93 <211> 434 < 212 >
DNA

<213> Ctenocephalides felis <400> 93 ccaatctttttaaaggtaatggcttatcttctgtgatacctcttgctgtggaaagagaag 60 caacttgatcacaataaacatcagcacctaattcttccatatcaacttctacatcagatt 120 ttgctatttttgtggcttcatctacttctttcctaacttgcgtttccatttccttcagtt 180 cttcaggtgtagctagttctgatgaaaccattctttcacggaatgaagtgattggatctc 240 ttgtctgtcttacttcttgaatttcttctcttgttctgtaactggtgccaggatctgaca 300 ttgagtgccctgaatatctataagtcatagtttctaaaacaattggaccttttccagttg 360 tgcaatgttcaattgcatattttgatgcttcacgcaccgctaacacatccataccgtcaa 420 FC-8-C2-PCT.ST25.txt cccaaattcc aggt 434 <210> 94 <211> 347 <212> DNA
<213> Ctenocephalides fells <220>
<221> misc_feature <222> (58) .. (58) <223> n = unknown at position 58 <220>
<221> misc_feature <222> (243)..(243) <223> n = unknown at position 243 <400> 94 aatggaaagaggatgtcacagaagcttatcctggcaaagggcaagcgctattccaagnat 60 tccagtggctgatgtggttgcaggaagccgaatctactgatgaggatggagaagcttaag 120 tgtggaaataagaaacaaaaaatatgaaaaactctgataaaaaggaattgtaagtttagg 180 tcacccttcatattgaaccaatataaatgcttaacatcttccaataaacgaattatatat 240 ggntttaatgaagtcagatgaaaggatgaacagcatattatctagaagaaaaactaaagt 300 atgctcgtcacacttcggaaaatgctaagaatgttttattgactcgt 347 <210> 95 <211> 440 <212> DNA
<213> Ctenocephalides fells <220>
<221> misc_feature <222> (38) .(38) <223> n = unknown at position 38 <220>
<221> misc_feature <222> (129)..(129) <223> n = unknown at position 129 <220>
<221> misc_feature <222> (134)..(134) <223> n = unknown at position 134 , <220>
<221> misc_feature <222> (164)..(164) <223> n = unknown at position 164 FC-8-C2-PCT. ST25 . tact <220>
<221> misc_feature <222> (188)..(188) <223> n = unknown at position 188 <220>
<221> misc_feature <222> (230)..(230) <223> n = unknown at position 230 <220>
<221> misc_feature <222> (239)..(239) <223> n = unknown at position 239 <220>
<221> misc_feature <222> (289)..(289) <223> n = unknown at position 289 <220>
<221> misc feature <222> (303)..(303) <223> n = unknown at position 303 <220>
<221> misc_feature <222> (307)..(307) <223> n = unknown at position 307 <220>
<221> misc_feature <222> (349)..(349) <223> n = unknown at position 349 <220>
<221> misc-feature <222> (417)..(417) <223> n = unknown at position417 <220>
<221> mist-feature <222> (419)..(419) <223> n = unknown at position 419 <400> 95 gaaaggacca ttaatttata ataattattt taatttgnga aaaaaaaaaa aaaaaaaaaa 60 gggggggctt gtacaagctt tctttttttt tttttttttt ttggctatat cgaaatttat 120 tactcaagnt tttntacagc tttaaacaat tattgatcct tatntttgcc attgtagtag 180 ggcaagtntt gtaaaactaa tttccaatca gtgaagtagc aaagggcatn tgcaccagnt 240 FC-8-C2-PCT.ST25.txt agaccaaaaccagcagacgttcctacccac tgttttgctt gctcaatgng tttaotcccg300 agntttntcaaaatactoattttgcttact taaatcaatg ctcctcggnt tcaataaata360 attttaagcgcagaatttagctttgcgcta ttgtttcaaa aaaaaaaaat aaaaaanana420 aaaaaaaaaaaaaaaaaaaa 440 <210> 96 <211> 498 <212> DNA
<213> Ctenocephalides felis <220>
<221> misc_feature <222> (493)..(493) <223> n = unknown at position 493 <400>
96 ctaattcaattgttcggggatgaagtgccagctctggaaccccttgaaca60 tttccttgtg acttcagaaaccaaatcagcaactctagcctgacttgtctgtgaaocagtttgtccaggt120 gtgttagcagaactttgaatgataagattttggttggcagcaagactctgtccacacata180 tttctccgtttatgagatgctaaatcattactttgggcaaaagctctaccacaaaccata240 caagcgtagggcttttcacctgtatgtgtcctcatatgtatagctaacttatcagatcgt300 gcaaatcctctgttacaaacactgcatacaaatggtttttctcctgtatgttttctcata360 tgcaatgtaagatctgtagttcttggataacctttaccacagattttgcaaaaatttgtc420 atttgaccagtatgtctccgcatatggacggctaaattacttcttgaactgcaactttta480 ccacaaacactgnataat 498 <210> 97 <211> 186 <212> DNA
<213> Ctenocephalides felis <220>
<221> misc_feature <222> (30) . . (30) <223> n = unknown at position 30 <220>
<221> misc_feature <222> (63) .(63) <223> n = unknown at position 63 <400> 97 accacgaggt gcaggcggaa ttccagttan tttaaaggtt cctaaacgat tgttatcttt 60 ggncataacc ctttctcctt catagacttg aattgttact gcaggctgat tgtctgcatg 120 agtgctgaag gtttgtgttc ttttacatgg aattcttgca ttgcgttcaa taatcttagc 180 FC-8-C2-PCT.ST25.txt cattgt 186 <210> 98 <211> 270 <212> DNA
<213> Ctenocephalides fells <220>
<221> misc_feature <222> (36) . (36) <223> n = unknown at position 36 <220>
<221> misc_feature <222> (40) .(40) <223> n = unknown at position 40 <400> 98 catattttacaactgtatgagtttggattaaaaatnaatncctatgtataataatatgaa60 aataactattataattac,aacaattatagttttgaataaaaatcagttataaaatcacaa120 aaactgatttatattttacagcaaaattgcttataattaagcttcttattataattaatt180 ttttatttcatttctttgcaagctcatttgataaccaatcgaggccttcatataaaccgt240 gcccttgtgttgcacatgtagcttgaatgt 270 <210> 99 <211> 279 <212> DNA
<213> Ctenocephalides fells <220>
<221> misc_feature <222> (23) .(24) <223> n = unknown at positions 23 and 24 <400> 99 agattaataaatttaaatatgtnnaacaaattaaactggcaaggaacttt gcactatgag60 cttattgatgtaatgatttttcactggtatcgttatctataagtaaaatg ctataattaa120 tcttaaaaggctaaaccggcttcagcataagttgtgaagatcttttcaat agagtttaca180 tatgctgctgttctcaagtccaatcccaaattgaatttcattgctgtctt cacgatagct240 cttgcagacctttccattgtgtagtccaagccagaatgt 279 <210> 100 <211> 193 <212> DNA
<213> Ctenocephalides fells <220>
<221> misc-feature FC-8-C2-PCT.ST25.txt <222> (31) . . (31) <223> n = unknown at position 31 <220>
<221> misc_feature <222> (33) .(33) <223> n = unknown at position 33 <220>
<221> misc_feature <222> (38) . (38) <223> n = unknown at position 38 <400> 100 cgacacgccc caagttaccg acctccagtg ntnatgcnca agtttcctgg aatcaaactt 60 aatataatct gtgatcttag aactggcaat atccacctga acagtatcgt ttactttgat 120 taaaggatct gggtagcgaa tggtgcggcc atcatgtgtt gtcaagaatg gaatattttt 180 gggtcccatt tgt 193 <210> 101 <211> 110 <212> DNA
<213> Ctenocephalides felis <400> 101 tcatgaatat caagagtcta ctataggtgc tgcgttttta actcaaactg tgtgtctgga 60 tgacacaacg gttaaatttg aaatttggga tacggctgga caagaaaggt 110 <210> 102 <211> 323 <212> DNA
<213> Ctenocephalides felis <220>
<221> misc_feature <222> (5). (5) <223> n = unknown at position 5 <220>
<221> misc_feature <222> (16) . (16) <223> n = unknown at position 16 <220>
<221> misc_feature <222> (23) .(23) <223> n = unknown at position 23 <220>
<221> misc_feature <222> (66) .(66) FC-8-C2-PCT. ST25 . tart <223> n = unknown at position 66 <220>
<221> misc_feature <222> (75)..(75) <223> n = unknown at position 75 <220>
<221> misc_feature <222> (82)..(82) <223> n = unknown at position 82 <220>
<221> misc_feature <222> (406)..(406) <223> n = unknown at position 406 <220>
<221> misc_feature <222> (436)..(436) <223> n = unknown at position 436 <220>
<221> misc_feature <222> (441)..(441) <223> n = unknown at position 441 <220>
<221> misc-feature <222> (471)..(471) <223> n = unknown at position 471 <220>
<221> misc_feature <222> (499)..(499) <223> n = unknown at position 499 <400>

aagcatcaggaggccactctgcaatggctaaaacagtcggtttcccagcagcaattgcca 60 ccaggatgattttggatggagaaatccaaaaacgtggagttgttttgccattcagtgaag 120 atattttataggccaattcttagcagattgagagctgagggacttcatgccacagaaacc 180 gttaaagttttgtaagatggaggagaaacactttgttttattatcgtgaattcattaatc 240 gattttgaaaatatgactggctcttttagcattatacatatttttaagctcgctttgata 300 tatcaagacataagtatttactt 323 <210> 103 <211> 462 <212> DNA
<213> Ctenocephalides felis FC-8-C2-PCT.ST25.txt <220>
<221> misc_feature <222> (23) .(23) <223> n = unknown at position 23 <220>
<221> misc_feature <222> (36) . (36) <223> n = unknown at position 36 <220>
<221> misc_feature <222> (426)..(426) <223> n = unknown at position 426 <220>
<221> misc_feature <222> (431)..(431) <223> n = unknown at position 431 <220>
<221> misc_feature <222> (442)..(442) <223> n = unknown at position 442 <400>

ttttgatgtcatataaatgccancaattaaaccaantagaccaatagcagaaccaaagat 60 ttcaacaatgagaattttgacaaatagtgcagaattggcagcatcagcaagagctgctcc 120 agatccaacaataccaacagcgataccgcagaataaattcacaagaccaacagccaaacc 180 agctccaaaaagaacataaccacccatccaatttgcttctttatatcctgctttttcaaa 240 aacagatttgtctgtgtattccgctaacagtcctgacagaacaattgctgtaattaaacc 300 ataaatagcaacagcttcacagaaaataactgaaattaagttttttgttttgattctggg 360 cgctttcacaccacctcctacaatggatgtgcctgtggtatgaattccaacaagctgctc 420 caacanctganaatgctacggntaaacaattcctaagggagc 462 <210> 104 <211> 484 <212> DNA
<213> Ctenocephalides felis <220>
<221> misc_feature <222> (16) .(16) <223> n = unknown at position 16 <220>
<221> misc_feature <222> (25) . (25) FC-8-C2-PCT.ST25.txt <223> n = unknown at position 25 <220>
<221> misc_feature <222> (31) .(31) <223> n = unknown at position 31 <220>
<221> misc_feature <222> (39) .(39) <223> n = unknown at position 39 <220>
<221> misc_feature <222> (89) .(89) <223> n = unknown at position 89 <220>
<221> misc_feature <222> (116)..(116) <223> n = unknown at position 116 <220>
<221> misc_feature <222> (287)..(287) <223> n = unknown at position 287 <400>

agccgtggaaaacctnacggaaccncaattnctttcggnagcccgcccagggacgactac 60 agggaacaaatgtcgatgaagcccgtttncttattaaggaaagcaaactaaagatngtag 120 cccaagatgatttggatgatgcagctgcattagttgtcagtttatctcattacatatata 180 ctttgcggttaatttaatgagatatgcatagtatatattaatctttcttttttttcactg 240 cacatgcaaagctaaatttagccagcagaatgtgttattcaacacangtcgtattgttta 300 gtcgtgtaatgtcaacatgtattgcccattttgattggattccttaatacaggatatcag 360 caccacttgttaagtttgaaactctaggtaggatatgatcatatttattataatgcacat 420 ttattatgttactaatgttcaaaatgattgatgatcgatgattgatatcattgtcagtaa 480 tagt <210> 105 <211> 373 <212> DNA
<213> Ctenocephalides felis <220>
<221> misc_feature <222> (55) .(55) <223> n = unknown at position 55 FC-8-C2-PCT.ST25.txt <220>
<221> misc_feature <222> (330)..(330) <223> n = unknown at position 330 <400>

gaggctcatcagcgtggtccacttctttggacggcaacacttcgtaaggttcctnggact 60 ctaacggatcactgagtccaacctcgtttcgcgcaaatattcgtatcaagtattcgttct 120 gctcctgtaaatccctgatagtaaaattctgaatagaagcctgaacgcgtcctacttcca 180 tccacatagttttcttaacatctctaatagctatgttgtaaccctcgatgggagcaccac 240 cgtcggattccggaattccccaacttatcaCaatattatttggacctacacttctcatct 300 ctaagggggcagttggaggagatggcacanttgcgtgagtttttagagtaacattccttg 360 ttactgcgggcgt <210> 106 <211> 231 <212> DNA
<213> Ctenocephalides felis <220>
<221> misc_feature <222> (60) .(60) <223> n = unknown at position 60 <220>
<221> misc_feature <222> (88) .(88) <223> n = unknown at position 88 <220>
<221> misc_feature <222> (119)..(119) <223> n = unknown at position 119 <220>
<221> misc_feature <222> 057)..(157) <223> n = unknown at position 157 <220>
<221> misc_feature <222> (192)..(192) <223> n = unknown at position 192 <220>
<221> misc_feature <222> (232)..(234) <223> n = unknown at positions 232, 233, 234 FC-8-C2-PCT.ST25.txt <220>
<221> misc_feature <222> (263)..(263) <223> n = unknown at position 263 <220>
<221> misc_feature <222> (280)..(280) <223> n = unknown at position 280 <220>
<221> misc_feature <222> (284)..(284) <223> n = unknown at position 284 <220>
<221> misC_feature <222> (342)..(342) <223> n = unknown at position 342 <220>
<221> misc_feature <222> (354)..(354) <223> n = unknown at position 354 <400> 106 tattccttgt ctggcacata atattttcgg ctttgatatc caaatgaatg atattctttn 60 ggtgcatatg gttaacacct tcacaaantt gtctcatgta atctatgact tctgcttcng 120 acatacgata accgtcagcg gtgatgcgtt caaacanttc accaccagat aagaattcca 180 aaatgagaac cntttcatca tcatcttcga atgcatcatg aagattaatc a 231 <210> 107 <211> 411 <212> DNA
<213> Ctenocephalides felis <220>
<221> misc-feature <222> (145)..(145) <223> n = unknown at position 145 <400>

aaaaaaatgcaagaagaagtgtaggcataatcactttccgcctccctctcgtatcggcta60 aaaatccccataaatgtgaactgcttataatacctataaaagctatagcgcttaatattc120 ctttttgttttgttgttaattccanatcacattgtgctgaaggaacgacaaaactcattc180 caagagtttccatcaaaacacatactaatacgcatccactcacgaccagaagaaggtaat240 tgaattttccaaaacctgtgagtgccaacgcatcttcaaactgccttggttttggttctt300 FC-8-C2-PCT.ST25.txt tggaagctat tatttgaatc cccgtgttac tgtccatatt tattaatttc caaactacag 360 attcaattaa accgttttgt ttaacagtct aacagtaaaa aaaaaaaaaa a 411 <210> 108 <211> 261 <212> DNA
<213> Ctenocephalides felis <400>

tctcggtggaatcgatttctgtagtttcgtcgttaactgacaatattagagccaataaat 60 atttcttagaaacatcaacagcccacgtagtctgtgcaagattggtagttggtatatccg 120 taatgataatctgaggttgttgctggtttaccgattgttgctggtttatcgattgttgct 180 ggtttagcaattgctgctgttgccagagaaatttttgttcctgaactttttggtattgtc 240 gcaactgctgttgatgtaagt 261 <210> 109 <211> 498 <212> DNA
<213> Ctenocephalides felis <220>
<221> misc_feature <222> (33) .(33) <223> n = unknown at position 33 <220>
<221> misc_feature <222> (62) .(62) <223> n = unknown at position 62 <400>

atttgtatgtaagaaataattcacatctattcntaatgaagaccaaatagataattgaaa 60 anccacaaaaacaacatccgtaggttcattattttgatcatatttaaatagcatgttgtt 120 ggtccacaaatcaccatgatttaatacatttatacagccaggacgcattttagttgaagc 180 cattgctctttggataattgtatccttcatattatataatttttttgaatatttttgata 240 gccatcccaaattgatgtagcttctgaaagctccaccagcgcttttaacagcaattgatt 300 catcagatggtcttcagattctgtttgtataaaacctaatgtgtaatcatcaactgaatg 360 aggatcctttagctccatgacaatggatgcagcatgcatatgtgcaattttgtccataag 420 taaagtagaatgtgcaatatctaggcctcttgtcctttttcccattttgtaaccacctat 480 tgccaaatccgtcataac 498 <210> 110 <211> 356 <212> DNA
<213> Ctenocephalides felis FC-8-C2-PCT.ST25.txt <220>
<221> misc_feature <222> (142)..(142) <223> n = unknown at position 142 <400>
110 tttttttttttttttttcctcttttattagtataataatatctacaatca60 tttttttttt tctaattatatcaaattaattattaatgatgtccatgaccatgatgatcatctttaggat120 tCattttttCCCaCgCCttCtntaatgcgactgttactactgtagcagccagtccccatt180 tgaatcctcggaataaaaagCCCatCgttCggctccttcttgttccaaattcttttgtat240 taaatcgccatacttcatttctcagccatgggtctttaagtcctttacttgcaagagcct300 tttggacttgaactaattcaggggcatcttcaactttgtaaatcctataatctggt 356 <210> 111 <211> 500 <212> DNA
<213> Ctenocephalides felis <220>
<221> misc_feature <222> (498)..(498) <223> n = unknown at position 498 <400>
111 tatccgcgaatgaaactaaatgagctttcaacattggacttaagcaacaa60 gctcattgag tgatttttcaatattaccagaacaactcgacataaatctaatgcccaatttgagacacct120 ttcactaagtggaaatccaattcgcaaaattaaatttgaaaatatcctttccttggaaac180 gctcgagataaacgatatgtcaagacttacagaaatttcgggactcgaaaacatacatgg240 aagaatatttgtcgaatcaaataaaccttgtgttcatctgagcatttcaaattgtgaatt300 gctggttaaaatacaaaatgatgcttttgaaaatataaatttatgtagtttaaatttgag360 tcataacgctctgaaagaagtgccgctctcggtgccaaacgacatagaacttgatttaca420 gggaaaccctttgatatgctcttgcagcctacaatggttttagacgatatattaccattt480 tgtataaacatcacccanac 500 <210> 112 <211> 189 <212> DNA
<213> Ctenocephalides felis <400> 112 tcatcgttca ggtcagggtg cttttggaaa tatgtgtagg ggaggaagaa tgtttgcacc 60 caccaaacct tggcgcagat ggcatcgcaa gatcaatgtg aaccaaaaac gctatgcttt 120 ggtttccgca attgctgctt ctggtgtgcc agctttagtt atgtctaaag gacatgttgt 180 FC-8-C2-PCT. ST25 . tact tgatcaagt 189 <210> 113 <211> 131 <212> DNA
<213> Ctenocephalides felis <400> 113 aacaagtgtc tctggtcata aattatgatt tgccttccaa cagggaaaac tatatccaca 60 ggattggtcg aggtggacgt ttcggtcgta agggtgttgc tataaacttt gtcacagatg 120 aggacaagcg t <210> 114 <211> 102 <212> DNA
<213> Ctenocephalides felis <400> 114 attccctgaa taagagggaa taatcgctct ccaagcatct gtttctgttc atgagccggt 60 gctgcggcaa gcatggaagc ggtgagtggt tcttggcctt gt 102 <210> 115 <211> 299 <212> DNA
<213> Ctenocephalides felis <400>

gcgaaagaacatgtcgtataccgtctgaaagaagtaatgaggactggcaggactcaaaaa60 atggcccttccagcgaaaaagaagcacatgcttatgatgggcctcccggcatggaacccg120 atggcgtcatcgaaagtaattgggatgaggtggttgataatttcgatgatatgaatttaa180 aagatgaattacttcgtggaatctatgcttatggttttgaaaagccttcagcaatccaac240 agcgagccatcatgccatgcatagtcggtcatgatgttatcgcacaagctcaatctggt 299 <210> 116 <211> 403 <212> DNA
<213> Ctenocephalides felis <220>
<221> misc_feature <222> (60) .(60) <223> n = unknown at position 60 <400> 116 cccggtattg ctgaccgcat gcaaaaggtt aatcactgcc ttggccccat ccaccatggn 60 agatcaagat catcgctccc ccagaaagga aatactccgt atggatcgga ggatccatct 120 tggcctctct ctccaccttc caacagatgt ggatctccaa ggaagaatac gacgaatccg 180 FC-8-C2-PCT.ST25.txt gacccggaat tgtccaccgc aagtgcttct aagcgctttc taccctctag caaacaccaa 240 agaaagtgcc gtccggaaga aatggcggtt aagaaattat gcgcggccca tcgcgctttg 300 cgcatcgtaa ttgcgcttcc ggtcaatatc accggatatg ctattgcacc agcgaccatg 360 tgcacaaaat tatttgtaat ttattaattt attattggac tgt 403 <210> 117 <211> 229 <212> DNA
<213> Ctenocephalides felis <220>
<221> misc_feature <222> (181)..(181) <223> n = unknown at position 181 <220>
<221> misc_feature <222> (185)..(185) <223> n = unknown at position 185 <220>
<221> misc_feature <222> (187)..(187) <223> n = unknown at position 187 <220>
<221> misc_feature <222> (192)..(192) <223> n = unknown at position 192 <220>
<221> misc_feature <222> (205)..(205) <223> n = unknown at position 205 <220>
<221> misc_feature <222> (207)..(207) <223> n = unknown at position 207 <220>
<221> misc_feature <222> (211)..(212) <223> n = unknown at positions 211 and 212 <220>
<221> misc_feature <222> (215)..(215) <223> n = unknown at position 215 <220>

FC-8-C2-PCT.ST25.txt <221> misc_feature <222> (217)..(218) <223> n = unknown at positions 217 and 218 <220>
<221> misc_feature <222> (222)..(222) <223> n = unknown at position 222 <400> 117 caggatttca tggtcttcat gttttaattg gaacttcttt tcttattatt tgtttactac 60 cacttagatt atttcatttt aatcctaaac atcattttgg atttgaagca gcagcttgat 120 attgacattt tgttgatgta gtatgattat ttttatatat ttctatttac tgatgaggtc 180 nattnancag anaggaaacc tgtanancca nntgnannaa anaaaaaaa 229 <210> 118 <211> 469 <212> DNA
<213> Ctenocephalides fells <400> 118 atttcggacg accccaaaaa taatatcgac gaggaaaaac atttgaaagt cgtgcaattt 60 aaggacaaca agcccgaaga cgacgcggtg gaaatttccg aatatttcag atacaatgac 120 gttatcaaag aaggcgatat tttagtgcgg gctccggcgg aacgacttat tcacaaacta 180 aaggacggcg acgactgcgt ctgcatgaac tggagggttt gcaaagggaa aataatcgac 240 ggtcacccgt gtcctcagag ttacatagac gtctgctgcc aaactgagca gaattttgat 300 gaagataaaa acgaggagca gttgacattt aggaaat tat tgttatcgaa acaataatca 360 tgatattttt tgtaacattt ataagtgcac ttgtgaaatg taccaaactt tgcaatttgg 420 taaataataa ataaacaaat ttgtttaaaa aaaaaaaaaa aaaaaaaaa 469 <210> 119 <211> 500 <212> DNA
<213> Ctenocephalides fells <220>
<221> misc_feature <222> (428)..(428) <223> n = unknown at position 428 <220>
<221> misc_feature <222> (444)..(444) <223> n = unknown at position 444 <220>
<221> misc feature FC-8-C2-PCT.ST25.txt <222> (460)..(460) <223> n = unknown at position 460 <220>
<221> misc_feature <222> (467)..(467) <223> n = unknown at position 467 <220>
<221> misc_feature <222> (475)..(475) <223> n = unknown at position 475 <220>
<221> misc_feature <222> (491)..(491) <223> n = unknown at position 491 <400>

gttcttacttatatctagattatcaggtttatcgagctctagaatacgtctaagaatacc 60 atattgtatactcatttattaaatggaacatatttttaaagtgaatcaaaataaaattcg 120 tatttgacactttggtatttgtaaacaaaaaatgagcagcaaatcaaccaatagtcacca 180 atatagtagtatctgtctagaagtgactcaaatgataaacacaactgtgatcataaaaat 240 tcaataaaagacgagaaaatcaagatcatgttaaatcaaagcatcgttcaagatctcgta 300 gtcgttcatatgatagaattgattcacatgatagattttctagtaaagaaattacaacag 360 agattcaaactttctcatagtatgaaaaagttccaacttagaatcatcccgttcaaaaac 420 attgcttngccttttccacacatnagccaccttggggccnaggatantagcatangcaca 480 aacttgaattngctgatcaa 500 <210> 120 <211> 218 <212> DNA
<213> Ctenocephalides felis <220>
<221> misc_feature <222> (186)..(187) <223> n = unknown at positions 186 and 187 <220>
<221> misc_feature <222> (197)..(197) <223> n = unknown at position 197 <400> 120 ctcgagtttt tttttttttt ttttagaaat ttactgaaac agaatacttt agattaatta 60 ttatatacag taaacaatct tgatgtgata gtttattttt gagcaatata tttatctctt 120 FC-8-C2-PCT.ST25.txt aggcaaaata ttattttatg cataactgta catttattat caataaaaaa tataatatga 180 ccacannttt tttaaanaaa aaaaaaaaaa aaaaaaaa 218 <210> 121 <211> 500 <212> DNA
<213> Ctenocephalides felis <220>
<221> misc_feature <222> (460)..(460) <223> n = unknown at position 460 <400>

gttattttctaatctgtaccatcacagtttactcgagaagttatggatgatttcaatttg60 ctgttagaattgccatgtccagataaaagaattgcagaaatattatacgaagtgttgatg120 atcgacgcggaaccttcccggagtaccgtgaaaaagaagtttacattagaaaacaacgtt180 ataaaagtgccgttgttcaagtccaaatgtgaaaaatttacgtgtcgccgtgaatggttt240 gtttgacaatttttatttggttctgcaaacgattgatacttttgggcccgcaaaccccac300 ctatgattactattttgctgatttaattgcttccaagtgattcttagcaattcaaaatgc360 cagaacacacacctgcccctacaccgagcagagcggtgtatggatttgcattatatttaa420 caatgaaatcttgttttttgcttatgttgtatggtctatngtacctgatgatattctctt480 atgctgaattatattatctc 500 <210> 122 <211> 500 <212> DNA
<213> Ctenocephalides felis <220>
<221> misc_feature <222> (490)..(490) <223> n = unknown at position 490 <400> 122 tataaatgat ttaattcagaagcaaggcagttatgtaattttttgaatatatgtgataag 60 aaaaacattt aaaattatattagtgttaagtagaatttacgtgaaagtaagatgtgggtt 120 tattttaata gaatttttgctgcggtgttgattatattggcagtgtcagcaagggaatcg 180 aattcccaaa aacttactgttgatggatttaaaggtgatcctatagaagaatttcgtcag 240 gaatattcta cgtttttaaaaaagggcgaagtatcaaagaagttacaaaaacttactgat 300 ttactattaa aaccgagtgatgaaaatgattgtgcaaaatgccaaattttggcaacattt 360 gcaatcgaaa gaagacgtaatgggacttccaatgcagagatgaattctttgatgaatgat 420 FC-8-C2-PCT.ST25.txt atgtgtataa gattaaatat atggatagga aaatgtgtga aaacatgata tatatgcatt 480 tggatatcgn ctgatataat 500 <210>

<211>

<212>
DNA

<213> felis Ctenocephalides <400>

ggatgataaggatcttgtattcatcaccaaccccgatcaaagagaaaaagattgtaaagg60 tttcttgatcaatttgattgattcacctggacacgttgatttctcatctgaagtaactgc120 tgcacttcgagtaactgatggagctcttgtggttgtggattgtgtttcaggtgtgtgcgt180 tcaaactgaaaccgtattgcgtcaagctattgctgaacgtattaagcctgttgtgttcat240 gaacaaaatggaccgtgcacttttggaattgcaattggactctgaagaactgtaccaaac300 tttccaacgtattgtggaaaatgtgaacgttatcattgccacatactctgatgatactgg360 tccaatgggtgaagtcgtgttgatcccagcaaaggttcagtggttttggatctggcttca420 tggatgggcttttactctaaaacaattctctgaaatgtatgctgagaaattcaagattga480 tgtactaaattgatgaacag 500 <210> 124 <211> 0 <212> DNA
<213> Ctenocephalides felis <220>
<221> misc_feature <222> (5). (5) <223> n = unknown at position 5 <220>
<221> misc_feature <222> (86) .(86) <223> n = unknown at position 86 <220>
<221> misc_feature <222> (134)..(134) <223> n = unknown at position 134 <220>
<221> misc_feature <222> (177)..(177) <223> n = unknown at position 177 <220>
<221> misc_feature <222> (189)..(189) <223> n = unknown at position 189 FC-8-C2-PCT.ST25.txt <220>
<221> misc_feature <222> (254)..(254) <223> n = unknown at position 254 <220>
<221> misc_feature <222> (285)..(285) <223> n = unknown at position 285 <220>
<221> misc_feature <222> (306)..(306) <223> n = unknown at position 306 <220>
<221> misc_feature <222> (332)..(332) <223> n = unknown at position 332 <220>
<221> misc_feature <222> (339)..(339) <223> n = unknown at position 339 <220>
<221> misc_feature <222> (411)..(411) <223> n = unknown at position 411 <220>
<221> misc_feature <222> (419)..(419) <223> n = unknown at position 419 <220>
<221> misc_feature <222> (485)..(485) <223> n = unknown at position 485 <400> 124 <210> 125 <211> 436 <212> DNA , <213> Ctenocephalides felis <220>
<221> misc_feature <222> (346)..(346) <223> n = unknown at position 346 FC-8-C2-PCT.ST25.txt <400>

cgccaacctcagatatagtggcaggtcctggttccaatattcgaataatgggtggttcca 60 actcgcgcctaaacgcacgatatgctaccaaacaacttctgatgagccatgctgagaaac 120 gaattaatcggaatagttactccacggatatttattaagttatttcataataagtgcttt 180 gacatgttagattaaaagctttattttctgaagcaactttaaattttaggaatttgtatt 240 ctacaaatgttgtagaacaaaacggatataaagatacgcacaaggtgcataaaatagaag 300 aaaatgaaaaagtaagccaaagatttagcaaatgttcaaatattgnagtttcaaatattc 360 tctcaattctttttgataaatatctaattgcgaattaataaaatgtctgaattattaata 420 ataaacagattagcag 436 <210> 126 <211> 500 <212> DNA
<213> Ctenocephalides felis <220>
<221> misc_feature <222> (6). (6) <223> n = unknown at position 6 <400>

ctggcnctttgtatacataccccgagaacttccgtgcttacaaatctttaatcgctgcca 60 aattttcgggtgctaatgttaaagtagcagccgattttgcattcggagagacgaataagt 120 cagaatcgttcctcaagaagttccctgcgggaaaggttccagcgttcgaaagcaacgatg 180 gaaaatacttaagcggcagcgatgctattgcttattatgtatcaaatgcccaactacgtg 240 gcgcctccgacttggaaaaggctcaaattctacaatgggtagattttggcaatggagaga 300 ttgtgcccccatcgtgtgcatgggtgttcccaattttgggaataatgcagttcaataagc 360 aaaccactgaccgtgcaaaggaagatgtcaaaagtgccctttccgtcctcaatactcatt 420 tagtaaataacacatatctcgtcggtgaaagaataactttggctgatatcagtgtgttct 480 gtcattacttcacctgtcca 500 <210> 127 <211> 500 <212> DNA
<213> Ctenocephalides felis <220>
<221> misc_feature <222>. (468)..(468) <223> n = unknown at position 468 <400> 127 gagttcttta ccctcactga aaattgcttt attttcctca aattgcataa tttaaaaatg 60 FC-8-C2-PCT . ST25 . tact cttttgacaatagttcatttgaacatgtgcacattttaaatactttattttatttaagtg120 tgatttagcttaattgttaatttttaattcatctactgtagactacaacaatggccaaat180 ttctggtacactcttcaatttttggttgtgtcagtattgtgagttatttcatcgggtgca240 atatcgaaagaaaaaatatattaaaccatttcaaatcccaaaacattgatttgaatgttt300 tgagcaaggtatgtacatacataaaaattggttgcggttggtagtccagaaaatcgcgaa360 tctgtgtaactgaggactggataaatgagattagttggtcatcctatacactgaacactt420 gaagaatataaaaattgtatttgtaattcacagatcaagaaaggctgnttaccgattttg480 gcactgttcagcagcaactc 500 <210> 128 <211> 500 <212> DNA
<213> Ctenocephalides felis <220>
<221> misc_feature <222> (6) . (6) <223> n = unknown at position 6 <400> 128 gcagtncggtaaaaacctcaccaaptaattcgctccatgattagatatccggagtgatta 60 cccttatcagtgcagttgtcgcaacgtggtgttagcagttgtggttatgtgcgtagtttt 120 ttagtacattagtgcccgtttgagtgttaatattgagtttttttttgcatcaaaaaggat 180 taatgacacgtgcttttccaagtatactttggcatgagggaatgtcttgcgcgctgagcg 240 caacgtaatgcgaagaactcgtgctgcggtcatttgctgcagcagcagaaggccgcgtgg 300 gcggagacggcgcttcactggataatagaaaattcaaaatttttatctaaccatttgtct 360 taaaaataatcatacgaaatactctaccatttagtaattaatcttccttctgcaaacctt 420 caactgatcaatttgatatgtgatatactaatatctattatattatttaataactaagat 480 tttatgaatctgtattatat 500 <210> 129 <211> 500 <212> DNA
<213> Ctenocephalides felis <220>
<221> misc_feature <222> (4). (5) <223> n = unknown at positions 4 and 5 <400> 129 gatnnatccg atttcggttg tgcttgggct agtgtgttcc tgctatcatt tgatcttttc 60 FC-8-C2-PCT.ST25.txt cctccggaagaatttgaaaatgcaccagaccttacattactttgatacggagatggtgct120 gctctttgcattacagaattgccagcattgtttgtcggaccacttggccctacagctggg180 ggcggagtaaaacttgttctttgtggaaagtttggccctgcatattgacgaatgtttgaa240 ttttgtggaggtggatatctattttgtgtaggactggctgtattggctgtcactcccgca300 acaggaaatctttgcgccataataacttaacttaaatgcattcagaaaaataccttaaac360 ggtatcaatgattttcacaattcataattaaaataggtaaaaatgcataaccttataaac420 taataatacatcgaaatgattttgcagttgtataataacgatattctttgaagttggata480 aacacgaatattaaaactta 500 <210>

<211>

<212>
DNA

<213> felis Ctenocephalides <400>

aaaataaactcttacacatatttttagatatacagacaatatatttatatcttattacgt60 atacaatatgtgtatctatattataaatacatatattgcatcataaataatttggtgtat120 tctacatttgtcaatggtcctaaaattacaatcgatcaaaattaacacttcaaacctcat180 tcattcttgaaaatgtaacacaatattagaaaattaaactaaaaaagcatattttcacac240 gctattaaatttacacaaataacatcaagcaattaagcacttactttaccacatctaatg300 catataataaaaaaaaaaatctaagctaaggtttgtattattaatgtaattatttggttt360 ctattgcgcaattcatatttccaaggtgtcaattaacaagtgatctttaaattaaatata420 aactgttactttatattgttttaaactatcaccaaatttatgtgatgaagtctataattt480 ggtgattggcaaaaatttcc 500 <210> 131 <211> 494 <212> DNA
<213> Ctenocephalides felis <400>

tgtaactagaattttatttttgtattaatatttaatttaataatttgacatgaaaattta 60 tcgttcagctctctgatttcaaaggattctaagcaagactacttgtttaaacggtataag 120 ggcgactcctgaaaaaacgaaatgaagaaagtgaagcgagtcagtgatgtgggggcagtg 180 ggtccaccgccgacagcccctccatcctatatggctgagcctcaatatgcacctataccg 240 caaaatcaaaactttaatggaagtataaatcctaattatgggtacaatcaacaaactgca 300 aaccaaccatttgtgccatattccggtcaaccaaattacggacaacagagtcctgctcag 360 ttccatcaaacgccgtctaatccaatgatgtcttcgcaaataccaaatcaacaaattcct 420 ggtccccaaaatatgaatattccaccacaattctcaatgtttcaacaacccttggtcaag 480 FC-8-C2-PCT.ST25.txt gaatggcaat tcag 494 <210> 132 <211> 500 <212> DNA
<213> Ctenocephalides fells <220>
<221> misc_feature <222> (4) . (5) <223> n = unknown at positions 4 and 5 <400> 132 ctgnnacagtgaatgacataagatggcgactagtatatgtgtttctaggtgccagatatt60 gtttaaaaatgaaataaataggataggactgcgggcactgttttatacacttcagggctt120 taaagaatctcgacataattccatccacacgtgcgcctcttcacacccaacgctagtgcc180 taatgatcgcaacaatttgacgcctgtcatcagcgcagctaaaaaatcatcggaaaatga240 ggctgtcgaggtgacgtacgctgccgggctgccacaaataaccgttccgctgccaagcag300 gcgagagcggtgccgattcacgttgcgaccggtcacaaacaccgtcggcgacttcatatc360 tatgctgcgctccgaggaccagggcgtcgatcgagtagtttgcgtaacctgctccggaat420 acgaattgctctccgacagcatcgaagctttactcgaacggccttcttttggtcatcaac480 gacaggaagttgaggtagat 500 <210> 133 <211> 500 <212> DNA
<213> Ctenocephalides fells <220>
<221> misc_feature <222> (4) . (4) <223> n = unknown at position 4 <220>
<221> misc_feature <222> (10) .(11) <223> n = unknown at positions 10 and 11 <220>
<221> misc_feature <222> (17) .(17) <223> n = unknown at position 17 <220>
<221> misc_feature <222> (36) . (36) <223> n = unknown at position 36 FC-8-C2-PCT.ST25.txt <400>

atgntttatnnctaaangtgttcaatgcattctgantttgcgttgatgagaagtgattag 60 tatcaaaaatttataaaatatgtggaagtttattcaattcacgatatttttcagcgtgct 120 gcgatttggtgcaagtttggagtgttacagttgtgaacttgcaaagaattcgaactgcac 180 agttcctctggctggtactctaccagttgcaacaaagcacgaccatattctaggaacaaa 240 atttttcattcatttttgaaagacaattcgacatatgatttagatgaacttgagaaaaac 300 aaagaagaagaaagcttccgatgtctgttggttactggagaaagagttttcaatcatgga 360 tttatagaaaaaggtgtgattagagaatgtgtccctgccgagtcacatgtgaagaaataa 420 agaaagatttaacaaataaaggagcccgggatctaaattgcaccatgtgcgacactgact 480 tggtaatgggtcttagacct 500 <210> 134 <211> 0 <212> DNA
<213> Ctenocephalides felis <400> 134 <210> 135 <211> 500 <212> DNA
<213> Ctenocephalides felis <220>
<221> misc_feature <222> (4). (4) <223> n = unknown at position 4 <220>
<221> misc_feature <222> (7). (7) <223> n = unknown at position 7 <220>
<221> misc_feature <222> (35) .(35) <223> n = unknown at position 35 <400> 135 gatnttntcg aacaattcaa tctaccaagt ttatngcgaa caattgataa taaaaaataa 60 ataaataatt acttgtgtcc ggatgaacga atcatgctcc taaccccatt tggtgaagat 120 ggactctact aaaccttttt gtgaataatg aacagttttt gtcagtgttc accaactatc 180 aacccccgat actaattaag tgcgactgat cattaattga catgtctcga gtgatttacc 240 caaagcaatt aactaattaa ccaattactt aagtacagct aattacaaac ccagcgaaag 300 FC-8-C2-PCT. ST25 . t~et gtacacatac ctgcacaact gcatatactc acatttcaat aatatgaata tttcctgttc 360 atgtaaacca ctcaattaat ttaatcactg ataaattgaa catttaaatt tccttcttga 420 atacgaataa atttacctaa attagagtca cggctgttta ttaaacttta attatcaatt 480 aatcaaaaaa tgccatgcca 500 <210> 136 <211> 549 <212> DNA
<213> Ctenocephalides felis <220>
<221> misc_feature <222> (6). (6) <223> n = unknown at position 6 <400>

aaagtntaatatatatcttttaatataccgtttgtttcatatataaaacattaataaaat60 ctaagataaacaataccaagttactatcatagataagtaaacataactatactacggtta120 ctatatgtacttgagttcactagaattttgttttctataacaaatgctacagaagtgtag180 tattaaaaaacaattaggttctcgaaaaggcgcggcatcttcagatttccaaattgcatc240 taataattcactgcaactgactttccaatcatctgatgtcacagacctcaaatttcttct300 agaaactgaaagatacatctattcaatattggaaagaccatttttaattttgtaatgtta360 tatgaacaaattttatatactaaaattgaatcatttacagtaattttcaaattaacccga420 gattcacataatcacgctcataacacattcatgcacatgggaaaatttcaagtgtcaggg480 acttggtaccttttctactatcctcaaatactacttcttggatcattatcattttctggt540 tcgctctct 549 <210> 137 <211> 549 <212> DNA
<213> Ctenocephalides felis <220>
<221> misc_feature <222> (7). (7) <223> n = unknown at position 7 <220>
<221> misc_feature <222> (499)..(499) <223> n = unknown at position 499 <220>
<221> misc_feature <222> (523)..(523) <223> n = unknown at position 523 FC-8-C2-PCT.ST25.txt <220>
<221> misc_feature <222> (537)..(537) <223> n = unknown at position 537 <400>

gtgcttntatgctatttcaataaatgtttgttgaatgaaaaggaaatttattgttgtttt 60 gggtgcattgttttggcactagtgataaaaacttaaatgaaatttgtgtgtttataatgt 120 gattttctttaattaaaaattatttaaattctctggtgtatgttaagttgatcaaagtaa 180 acatgaaaccttatcatcgaacattagttaattattgatcataaaataatatttggtgaa 240 atgacctaaaaattcaataacattttgcttaaacaatatttatcactacataaaaaacat 300 gctatgaattattgcgttgattaagttccgacactttgctatattatttaataaatattt 360 tatttattttaattgtgattaaaaacttttatcgggtaattgctaaacttaattcgatat 420 tttttgctaacgcatttttgcggaatggctggatttatagaagttgccatatgtttggct 480 attctttactggctattcnacgcaagaccgccagcaatggtangtgtagtggcagancga 540 atgtggtct <210> 138 <211> 392 <212> DNA
<213> Ctenocephalides felis <220>
<221> misc_feature <222> (33) .(33) <223> n = unknown at position 33 <220>
<221> misc_feature <222> (380)..(380) <223> n = unknown at position 380 <400> 138 agatttgaaacaaaacaatttgaagataaagtngtacttacacagaatgaaaaggatttt60 gaaaaaaatgttgaaaagaaagaagcaaacgtaaatttagaaacaaataaggaaacatct120 gtgaaaaataaacttaacgaaggatttataaaaatcaaagatttttttaaattttaaatt180 ttattttatcatccaattttatttaatttttatgtcatgtatcatcaaataatgaaatga240 aagcatgcattaggataaatgtcaacatatatatagcaaagtcgttacatattttgttaa300 taatatgaaatacatttactatggtattaatttttgttttagatactttatttagaaata360 ataaatataaaagacccggnaaaaaaaaaaas 3g2 FC-8-C2-PCT.ST25.txt <210> 139 <211> 549 <212> DNA
<213> Ctenocephalides felis <220>
<221> misc_feature <222> (5). (5) <223> n = unknown at position 5 <220>
<221> misc_feature <222> (7) . (7) <223> n = unknown at position 7 <220>
<221> misc_feature <222> (517)..(517) <223> n = unknown at position 517 <400>

gtcantnataaaaaaaaaatttttttcaaaaaaaaaaaacaaaaaaaaattaaaaaaaaa60 ttttttttaaaaaaaattttttttcaaaaaaaagtaattttttgtccaatttatagtttc120 ttaaaaatcgtattttttcgaagtaaattttttttaaaaatgtcaatcgtttttaaaaaa180 ataataatttttggtacgggaagttccagcaatttgccttaaggcacgatgccttaattg240 ccttaagacagaattccacttacagctagtttttacttgatttgatagaggaatagttac300 agcaactatccaggtttattacgtataaatatgatcagtcgatttcaagatatattaatt360 tagaagaaattgttacaactagcccctatctagcttccctacttagatatgtttacttag420 atattcccttccctacgtagatatttttccatgattagaaatttatttataagtaaattt480 tcttttttattcgttagtattaaatataaggttatgnatttatatctttgggataggtat540 acatgagta 549 <210> 140 <211> 115 <212> DNA
<213> Ctenocephalides felis <220>
<221> misc_feature <222> (3) . (3) <223> n = unknown at position 3 <400> 140 aantttagtt acatagttat atagaggatg agaagtttaa gtttgtagct tattttatca 60 ataaatgttc aaaattatga atcgatatat tcataactca aaaaaaaaaa aaaaa 115 <210> 141 FC-8-C2-PCT.ST25.txt <211> 549 <212> DNA
<213> Ctenocephalides felis <220>
<221> misc_feature <222> (537)..(537) <223> n = unknown at position 537 <220>
<221> misc-feature <222> (540)..(540) <223> n = unknown at position 540 <400>

attttcaagtacccattcgctcgatctcgaagccgcacgcagacatttttatttaaataa 60 cgattatgtagcataattgaataatttttccgaattgtaataatgtttttatcttccgat 120 attgataaacattgacctgatgttcctgcaaataagattttcttttctaaaacgaaacct 180 gaaagataccacatacttttcctcatgcatgtaagataaagttttgtggaattgttttaa 240 aataaaggcaagatgcggcgaagtactactaaacgttctatggtgactgattacgatgat 300 agaagtctggggccagtgcttaaaaaatccaatgctagtcctgatttatcaatggtcaaa 360 cagtacacatcaattagccctcagcagactactgttagttatcatttggtggatcatggc 420 tacggtgctactcctcaacatcaaattatgcgggacataccagttgctccacctaagacc 480 acagaggctgtcaaacgtcgacttaacttgacatgacaagttcagtcccagttcctnctn 540 ctcgaccaa 549 <210> 142 <211> 375 <212> DNA
<213> Ctenocephalides felis <220>
<221> misc_feature <222> (68) .. (68) <223> n = unknown at position 68 <220>
<221> misc_feature <222> (210)..(210) <223> n = unknown at position 210 <220>
<221> misc_feature <222> (253)..(253) <223> n = unknown at position 253 <220>
<221> misc feature FC-8-C2-PCT.ST25.txt <222> (292)..(292) <223> n = unknown at position 292 <220>
<221> misc_feature <222> (329)..(329) <223> n = unknown at position 329 <220>
<221> misc_feature <222> (366)..(366) <223> n = unknown at position 366 <400>

agagcagaggggcattagaaaataaactattagcagctgctgaagatctgagaaaaactt 60 taagaganggtggtaatttgactaatggcgaagttatggatccttggttccttgaaaatg 120 ttgatgctgacattgaattccaaacattgccaagttaaacggagctcttgccaatgtaac 180 aagtagagggtttgtctagtttcaacatanatcacataaaagtgaatgtgctcttcatga 240 aagctactttcantatcactttttgaccacctcatggcaaagggtctatacnacatttga 300 aggcaagttggcagatttgctaagttgtntggcaaaggctcctttgatattcatgttacg 360 tgatantaaaccgct 375 <210>

<211>

<212>
DNA

<213> fells Ctenocephalides <400>

gaagtttactaacgctttgataaagtatcctaaataaaagtataacggtggtttttgatt 60 gaattaattgtaaaatcaataagatataaagttgttaaataaggtaatctgtgctagaaa 120 tgtatattgtgctccggagtcgagaaataatctaaagtttagccgtttgtcaccgagacg 180 ctatttctgacagttacatatcaataaaagtatcatatcttgttctgattcgtgaaattt 240 acaataatgtctgagttaatgttatgttctgaacactgataattactgtttagtgttttt 300 caatagatttcctcaaaactgttctacagcagtggactcagaattgtgctaaaagcaagc 360 cgaattcggctttaataaaaaagtgaaaattattgaccttccatccgagaacgatacttt 420 tcacaatcagtcagactttctgtaatgtacccaaatatcttccaagagcacacaccaaag 480 aaataagcttcgttctccccgttacttgtacactcaaaaaaaaaaaaaaaaaa 533 <210> 144 <211> 414 <212> DNA
<213> Ctenocephalides fells <220>

FC-8-C2-PCT.ST25.txt <221> misc_feature <222> (284)..(284) <223> n = unknown at position 284 <400>

tgcaaaatttagtgaacaatcgccaaagcaaaatgtatagtgttagagactcattagttt60 tagtattgcttgcttgtctggtcgcgccaagcttacaggccactcctacctacaaagctg120 gcgtcgtagaatactatccgtcccagaatgagaaattcgaagagaaaattaatgaaaatt180 tgaagttttatgaaaaaacaattgaggaagcagaaggcaagaaggtagacatcctagtgt240 ttcccgaagcaaccttaaatggtggtatcaaaactagagaagangccaaggaacatggtg300 ctgagatcaaatttccaaataaagacagtttaggcccaacaaaattaaaacagccgccgt360 cttttttggaaaaattatcctgtaaagctatggaaaagaaatctacgtagttgt 414 <210> 145 <211> 350 <212> DNA
<213> Ctenocephalides fells <220>
<221> misc_feature <222> (4). (5) <223> n = unknown at positions 4 and 5 <400>

gaanncatctatcaaaatggccaaacgtaccaagaaggttggaattactggtaaatatgg60 aactcgttatggagcttccttacgtaaaatggtcaaaaagatggaaataacccagcacgc120 caaatacacttgcacattttgtggaaaggatgccatgaaacgtagttgtgttggaatttg180 ggtcttgcaaaagatgtaaaagaacagtagctggtggtgcctgggtatataccacaactg240 ctgctgcctctgtccgatctgctgttaggaggttgagagaaacaaaggaacagtaattta300 tattgatcttaataaataattaataaaaattaaaaaaaaaaaaaaaaaaa 350 <210> 146 <211> 217 <212> DNA
<213> Ctenocephalides fells <400> 146 cccttcagta tttcttttat tttcaattac gaaaatcaga aaacagttat tccaagatca 60 tggttcttgt tcccttcttt aagtttattt cctatcctaa agtgttaact attactacct 120 aaactgcgat gatatttgtg atgaatgatg ttgacatgat gtccgaagac gttggctaag 180 atgcggtaaa attctgttcc tggtcatcat cgttccc 217 <210> 147 <211> 489 FC-8-C2-PCT.ST25.txt <212> DNA
<213> Ctenocephalides fells <400>

catttgcaagaattatcagtgtaatccaaaaacaatcctgtccgcacgcaagcaaagggt 60 tgcgatggtcctggtcctggtggcggtggtggaggtgcagctgtcgccaagcactcggat 120 aaaggcgcttgcacgcattgtgggtaaagcgcgctgaagtatgtctgtccggcgcatgta 180 aaacgacccacgttgaaatttcctccagagagtctcacacattcaaaatatactttgcat 240 gttttgtctgctggatctgcaaatgagccgctctgcacgcagctgtatatgggcggcggc 300 gtaggtggttgccaaggcgctggaggatttcttaggcactccgaaagggaagcgaccaca 360 cattgctgatagaagtaagtagaaccaaggacaattgtaacgcgctacactgaatcctcc 420.

gcctgctttcaaagcacatttgtagtatcctttgcaagtgctgtcatagggatctataaa 480 catcccttc 489 <210>

<211>

<212>
DNA

<213> fells Ctenocephalides <400>

aaaaggtttacaagtctcaaagattccgtgcaggtaaaggtaaaatgcgcaacaggcgtc 60 gcatccaaagacgtggtccattgatcatttacaaccaagaccagggattgaagaaagctt 120 tccgcaatatccctggaattgaattaatcaatgttgaaagacttaacttgttgaagttgg 180 cacctggtggtcatgttggtcgtttcgtgatttggactgaatctgctttcaaaaaactag 240 atgagttgtatggaacctggaaatcgtcatcaaaatctaaagctggttacaatctaccac 300 aacctaaaatggctaacactgatttggctcgcttgttgaaatctgaagaaattatgaagg 360 ttcttcgtgctacagttactcgcagggttaaaaaattgaacccattgaccaatgcacgag 420 ccatgcttaaattgaatccctatgcagcagttactaaacgtaaagccattcttgctacta 480 agaagaagc 489 <210>

<211>

<212>
DNA

<213> fells Ctenocephalides <400>

agttaaggctctatattgctggctacctcttgctgtcaaaggtgcgaagccaggcatgaa 60 gaaatgtaaacgtgggaatggaaccatgtttacagctaattttctcaaatcagcattaag 120 ttgaccagggaaacgaaggcaggtggtgacaccagacatggtaagagaaactaaatgatt 180 caagtctccatatgttggagtgctaagtttcaatgttctgaaacaaatatcatacaatgc 240 ttcattatcaatacaataggtttcatcagtgttttcaactaattgatgt 289 FC-8-C2-PCT.ST25.txt <210> 150 <211> 171 <212> DNA
<213> Ctenocephalides fells <400> 150 tgcttggaga aagtgcagtt ggaaaaagtt ctttggtgtt aagatttgtc aaggggcagt 60 ttcatgaata tcaagagtct actataggtg ctgcgttttt aactcaaact gtgtgtctgg 120 atgacacaac ggttaaattt gaaatttggg atacggctgg acaagaaagg t 171 <210> 151 <211> 413 <212> DNA
<213> Ctenocephalides fells <220>
<221> misc_feature <222> (405)..(405) <223> n = unknown at position 405 <400>

acacaattagcaataggaaaatctagccacaaatatttttaacaatttatttaatactta60 tgttctagaaatacaaagtgtaaagttcaaggtttgtatagcttaataaagtaaacattt120 taaaatgtaatgaatttatctgcaa~tagtcaaaatcaaaattactatataatgattatt180 gaaataatgcttttatagattgcatttcaatctgtattaaattgtatgtaaatcacagca240 caagatctaacacacaattcatctaacagaaagaacaaaataattttaatgttattaaaa300 attacatataaaataacttttacaataaagtaaccttacatgagatgtgttgatttcaga360 tacttttcatacaacagtgctttctgtataaacaatataatatanataacggt 413 <210> 152 <211> 418 <212> DNA
<213> Ctenocephalides fells <220>
<221> misc_feature <222> (402)..(402) <223> n = unknown at position 402 <400>

ttgacaagcaaaataaaatacatcaacgttgttttttacggccacttgcaagttatttaa60 tggttccattttttgaacaactccatttgttccaagaaccaatgatgtttcatatgtttg120 tgatggttgtaaattcggaacttgtaatggtgcaccaggtgccagaccaaagctgttttt180 attcaactgtatggcaaaaccactcatagcttgcattgctttgttggtgtagctcatgtc240 catgtaaatctgtccacttctcctggagaatgttccatatatttccaaacctttgccctt300 Page 81 ' FC-8-C2-PCT.ST25.txt ttcagctggc aaccacaatg cttttggtat aacaaaaggg ccagctgatc ccacagagaa 360 tatttctccc agaagtccag agttaccgat gttattaagt anaattgaca tctgatgt 418 <210> 153 <211> 340 <212> DNA
<213> Ctenocephalides felis <220>
<221> misc_feature <222> (196)..(196) <223> n = unknown at position 196 <220>
<221> misc_feature <222> (285)..(285) <223> n = unknown at position 285 <400>

gaatactggttcataaaatttagaagccctaatttaccgcctacggcgatatgatgatca 60 gacatatgtccgggcttgtccaaaaatgtaaaatgcacatgggaattttccaatggcgtc 120 cacttatgaataatattgggaagtcctagcaattgcgttatcaaattttcctgcacgggt 180 tgaaaacaatccttangcatcttcaaatcatcccttacaattttaccctggttaattaat 240 ttggtaaactcgtcaccggatttgtcgagtgtcttaactttatcngcgatgctggttctg 300 cacgcgtccaattttcggcgtgttttgtccaaatcttggt 340 <210>

<211>

<212>
DNA

<213> felis Ctenocephalides <400>

tacgagtgcacattaaacgctcgaggtgtattcgacgtggcccgatatgcttgtgcagct 60 gagttatatttcaacagcgttctgcagcagtgcgtacctgcataccagtcagactgttta 120 ggtgcaagtgtcacttcttCCCCatCCataCCatCCttaCCatCCttaCCatCtttCCCa 180 accttgccaacatcttccccaacagcaggatttccttttggccgaaaatctcttgatatg 240 caaacaaaaactggaactaaatgtacaaaaggagaagtttctaaagacgatttaaattct 300 acatgtacctagtgagtgtgttatatgaaattgctattgcatattacatacaagcttaat 360 aaaggttatggtgatttatttcatttaaaggcaatgtatcggtttaatggttttaaattt 420 atttttatttaatattaaatagattaattaaaaatctataaagtgataagaggctgatat 480 ccataatattattgaata 498 <210> 155 FC-8-C2-PCT.ST25.txt <211> 102 <212> DNA
<213> Ctenocephalides fells <400> 155 agaccaagta aaaatctcac tcacagcttt taccttatta ggatcattta aaaatctatc 60 tagaatgcca ggttttgcca gagattgctg cactttctta gt 102 <210>

<211>

<212>
DNA

<213>
Ctenocephalides fells <400>

aatttgcggaaacacctcagattttagcggcagcggaagaaatttgtggaccttatgtct60 ggggccgttatgatttacttgttttacctccttcattcccttatggtggaatggaaaatc120 catgcttgacatttgtaacacctactctattggctggcgacagatcgctagtgagtgttg180 ttgctcatgaaatagcacatagctggactggaaatttagtcacaaattgcagttttgaac240 atttctggctaaatgaaggtttcactgtatttgtagaatacaaaattcagggaaaattgc300 atggtgaagatgttagagactttcattcgctttgcggactcacaacattgaaagaagagg360 ttcaactattaggtgaaaccaatcaactgactgctctcgttgttaacttacaaaatttaa420 gtcctgacgatgcattttcttctataccttacatgaaaggct 462 <210> 157 <211> 194 <212> DNA
<213> Ctenocephalides fells <400> 157 atcctttttt tctatgaacg gttttaggta acggagaata atgtaaggca tcactaaagt 60 cgtttttatt tgtgatttca tgattaggct cgggtcttct gacccgcata aattgacccc 120 gcaattttgc ttcctttgcc tcgtttgatg atacagcccg ccaatccaaa aataataatt 180 ttggtaaatg atgt 194 <210> 158 <211> 265 <212> DNA
<213> Ctenocephalides fells <220>
<221> misc_feature <222> (58) .(58) <223> n = unknown at position 58 <400> 158 tttttttttt tttttttttt tttttttttt ttctgcatca gataatacct cataagcngg 60 tcctagatct tgaaacttcg aggctgcatc tggatcgttt ttattcttat caggatgcaa 120 FC-8-C2-PCT.ST25.txt ttcctttgct tgtttcctat aagctttctt aatttcattt aacgtcgcac tacgagatac 180 ttttaatatt gaatagaaat ctcgtccagc gctggctagt aaaaaataaa tacttaagtt 240 aactaacact aaataacaca gtttg 265 <210> 159 <211> 498 <212> DNA
<213> Ctenocephalides felis <400>

cttggcatctagttttgtaaattcagtttggctgtttattttgctgcgatatttcaatgg60 atttttcgtatctggaggaagtgcaacaatatatgcatatttaggagaatttcataatcc120 taggcatcgcagcagggctattatgggagcgtcaagcatcttcggatttgcgtgtcttgc180 attaccgacggttgcatggttaattataaatcagaaatggtcattctatattgacttttt240 gggatatacatacaagccctggaggttgtatatggttgcatgtggtttgccatcactgct300 ttgttgttttgctttgtggaaattaccagaaagtcccaaatttttgatgaatcagggaag360 aaacgaagaagctcgtcaaattattgccaaaatgtatagaattaatactggtaaaccaga420 aagtgaattccccgtatcatcaatcttagatgaatatccaggagtggatggtgaaaatac480 aaataaaacaaagaaatc 498 <210> 160 <211> 617 <212> DNA
<213> Ctenocephalides felis <220>
<221> misc_feature <222> (573)..(573) <223> n = unknown at position 573 <220>
<221> misc_feature <222> (577)..(577) <223> n = unknown at position 577 <220>
<221> misc_feature <222> (579)..(579) <223> n = unknown at position 579 <400> 160 ccatttgcaa ggaattatca gtgtaatcca aaaacaatcc tgtccgcacg caagcaaagg 60 gttgcgatgg tcctggtcct ggtggcggtg gtggaggtgc agctgtcgcc aagcactcgg 120 ataaaggcgc ttgcacgcat tgttggtaaa gcgcgctgaa gtatgtctgt ccggcgcatg 180 FC-8-C2-PCT.ST25.txt taaagcgacccacgttgaaatttcctccagagagtctcacacattcaaaatatactttgc 240 atgttttgtctgctggatctgcaaatgagccgctctgcacgcagttgtatatgggcggcg 300 gcgtaggtggttgccaaggcgctggaggatttcttaggcactccgaaagggaagcgacca 360 cacattgctgatacgtgctgctgaagtaagtagaaccaggacaattgtaacgcgctacac 420 tgaatcctccgcctgctttcaaagcacatttgtagtatcctttgcaagtgctgtcatagg 480 gatctataaacatccctttctgcacgcatgaaaaaagaagcggcggtgctggtgtaattg 540 gccgtagtcgtagtaatcggacaattggaggangcanangtgtaattaaacattccgaat 600 atgtagggcccacacat . 617 <210> 161 <211> 450 <212> DNA
<213> Ctenocephalides fells <220>
<221> misc-feature <222> (3~4) . . (34) <223> n = unknown at position 34 <220>
<221> misc_feature <222> (65) .(65) <223> n = unknown at position 65 <400> 161 agaattagaattgacacaaaatgctttagtgaangaaatgaaattaaaattgatgattat60 tgatngattttataccaaatgaaatcaaagataaattttatgcaaatgcaaagtttgatg120 aagatttggacatatggacagttgcaataactaaagaaatgttgccaccaagacgaccga180 tatccaaaccaggacggcgcaggcctatttctgaggtctctttggttaaaagacattacc240 ctggcttggcaggtatacgtcacagaggtgagaatattattgagtatgacttgggcatgc300 catttcgcaccacatttgaatacgaagccccaacagtgtccccagcgttacaatcagtat360 tagaagatgctttacaaccagaaggagatttagaaatcacttcaccccgtcaaccgtctt420 cagactgcccaaatcatgaaaagacctagt 450 <210> 162 <211> 420 <212> DNA
<213> Ctenocephalides fells <400> 162 ctgaattcca gatgtagtga agtaaggaat ctcaaatttc acttgtattg ggggttttcc 60 ttccgtatct tcacattcca cacttggcaa tccaaaatgt gctctcatta ggtattcttt 120 accacctgga aatgatttta tggaccaagt aaatgcattc aaatcaggag catatttaac 180 FC-8-C2-PCT.ST25.txt acttccaatt gttgttttaa acttcgggga atctgcatca gctggtacag gaatcagtat 240 ctccacatta tttgcagttg accgtctttt aaattgtgac ttagctttga tcatgtattc 300 tacacggctg tgtgcgtgcc tttcaattac tgattcaatc catatcaagg gttttacatg 360 tgtgttaagt cgataagaca ttaactcaaa ctctccatca ggaggaataa atgatatggt 420 <210> 163 <211> 498 <212> DNA
<213> Ctenocephalides felis <220>
<221> misc_feature <222> (27) .(27) <223> n = unknown at position 27 <400>

tacaatgggaattgtcatgttcaaatncttggctgcggcaattataccttcggctataac 60 atcacacctcattattcctccaaatatattcactaaaatagcactgaccttaggatctgt 120 tgtgataatcttgaaagcttctttgactgtagaagcagtggccgcaccgcccacatctaa 180 aaagtttgctgggtctcctccatgtaatttaattatgtccatagtagccatggcaagacc 240 agcgccattaaccatacatccaatatttccatccaatgctatataattcaaatcaaattt 300 tgaagcttctacttccttaggatcttcttgagtccagtctcttagggcaaaaagttcttt 360 ttgtcgaaaatgagcattgtcatcaaagcgcattttgcagtctaagctgaaaaatatatc 420 ctcagatttccttgatatttcttttgaatcttcagcaaatggattgatttcaatgagtag 480 tgcatctttctttaaaaa 498 <210> 164 <211> 0 <212> DNA
<213> Ctenocephalides felis <220>
<221> misc_feature <222> (12) .(12) <223> n = unknown at position 12 <220>
<221> misc_feature <222> (70) .(70) <223> n = unknown at position 70 <220>
<221> misc_feature <222> (74) .(74) <223> n = unknown at position 74 FC-8-C2-PCT.ST25.txt <220>
<221> misc_feature <222> (87) .(87) <223> n = unknown at position 87 <220>
<221> misc_feature <222> (108)..(108) <223> n = unknown at position 108 <220>
<221> misc_feature <222> (125)..(125) <223> n = unknown at position 125 <220>
<221> misc_feature <222> (129)..(129) <223> n = unknown at position 129 <220>
<221> misc_feature <222> (157)..(157) <223> n = unknown at position 157 <220>
<221> misc_feature <222> (160)..(160) <223> n = unknown at position 160 <220>
<221> misc_feature <222> (163)..(163) <223> n = unknown at position 163 <220>
<221> misc_feature <222> (206)..(206) <223> n = unknown at position 206 <400> 164 <210> 165 <211> 373 <212> DNA
<213> Ctenocephalides felis <220>
<221> misc_feature <222> (44) .(44) <223> n = unknown at position 44 FC-8-C2-PCT.ST25.txt <220>
<221> misc_feature <222> (50) .(50) <223> n = unknown at position 50 <220>
<221> misc_feature <222> (300)..(300) <223> n = unknown at position 300 <220>
<221> misc_feature <222> (334)..(334) <223> n = unknown at position 334 <220>
<221> misc_feature <222> (359)..(359) <223> n = unknown at position 359 <400>

caaaaatgggcacctcaactggtgcaaaacgtttttggcttttnttgggnccgtggtctt60 tagacaaacgtgccagagggcttttcacgaaaaccaaggggagggcattgtcattcc 120 ga gaagggcgcaagcaaaaagttctcaagggcagagttattgcagttggcccggcaaagaaa180 ccaactggtgaacatgtgcccctaggaatcaaaagtggtgacatggtttgctgccgaatc240 cgaggcccaaaagttgagctcgaagagaacaagaattccttattcaaagaatccgatatn300 cttgccaagttggatatcgacgaataaattgccnaaaatttaccatctttcacaatttnc360 ccaacacaaactg 373 <210>

<211>

<212>
DNA

<213> felis Ctenocephalides <400>

aaatctccgccctgcaatcttccccaaaccaaccaaccgatcaaatagga agccaactcc60 aagctgtccaagttctcccaacaaacctcggtttgcacacacacagacac caaaacaact120 atgctgtaccccaacaaaccaactttggatcctttcaccatcaccaacaa atcgcaccca180 caaatattgggggctactactccccccacggaattctaccaatctactac gataatgggc240 acggattgttgaattttaatcctttgaagcctcaaaactactacgcccag tattatcctt300 actaccagaacacgagacagtattatccttacaattttggatattattat catcacagtt360 tgccgaattcgcactactacaggcagcagt 390 <210> 167 <211> 500 FC-8-C2-PCT.ST25.txt <212> DNA
<213> Ctenocephalides felis <220>
<221> misc_feature <222> (6). (6) <223> n = unknown at position 6 <220>
<221> misc_feature <222> (8). (8) <223> n = unknown at position 8 <220>
<221> misc_feature <222> (10) . (10) <223> n = unknown at position 10 <220>
<221> misc_feature <222> (14) .(14) <223> n = unknown at position 14 <220>
<221> misc_feature <222> (17) .(17) <223> n = unknown at position 17 <220>
<221> misc_feature <222> (35) .(37) <223> n = unknown at positions 35, 36 and 37 <220>
<221> misc feature <222> (52)~. . (52) <223> n = unknown at position 52 <220>
<221> misc_feature <222> (56) .(56) <223> n = unknown at position 56 <220>
<221> misc_feature <222> (231)..(231) <223> n = unknown at position 231 <400> 167 aggggngntn tatnatnaat ttttctttga cgacnnnggt taaggggggc cncggncggg 60 tattttttca taatcattca atattaatta ttttattaat tacaatttta gtgggatata 120 ttatgagatc tttattttat aataaattat ataatcgtta tttacttgaa agacaaaata 180 FC-8-C2-PCT. ST25 . tact ttgaaattatttgaacaattctacctgcatttatattaatttttattgctntaccttctc 240 ttcgacttttatatttattagatgattcaaataggcctttaatttctttaaaagcaattg 300 gtcatcaatgatattgaagttatgaatatactgattttaataatatttcttttgattcat 360 atataattccatctaatgaattaaatttaaattcatttcgtttattagatgttgataatc 420 gaattatcttacctattaattcacaaattcgtattttaattctgctaccgatgttcttca 480 ttcatgaaccattccctctc 500 <210> 168 <211> 498 <212> DNA
<213> Ctenocephalides fells <220>
<221> misc-feature <222> (30) .. (30) <223> n = unknown at position 30 <220>
<221> misc_feature <222> (57) .(58) <223> n = unknown at positions 57 and 58 <400>

atatatcttacatcgaatctaataacattnaagagttattaattttcatgtcgatgnntt60 agaaaatgattcagtagcaacttctgaattttctttgtaatcgataatactttctcaaag120 tataattgcatttcacaacacacgacggaaatatggttatggtccatatgaagcaatgct180 cgttcttctatccacttgcttctgataaatgttcggtataaagaatgcaatcactccaca240 tgctatcagagatgttcctgatattaaaaatgttaaatcgCaattataatctagaattat300 tcctacaacattgcttccaacaacacttcctaatcgacccatcattaaagatatacatac360 tgccattgccctgagttgtgtagggtacagatctactaatgccgcgtttaccactgtcac420 agcaattccgcaaaccagcaaccacaaatataaatatgatgctattgttaaatgttcaat480 aaatgcacaa attattcc 498 <210> 169 <211> 232 <212> DNA
<213> Ctenocephalides fells <400> 169 tgtaaacagc actttgcctt tgtaggtttt atcgaaggct tcgctcaagg ttttgatggc 60 tttggtgagt tcagttttgg ctttgtcggt ttcttcgggg gatttgaaat ttgttgctaa 120 gcggaagaag aataagctag gacgttcatc aggggttagt tctaagactt tgtcagataa 180 FC-8-C2-PCT.ST25.txt aagcgttgag gacaccaggt ctttcacaaa cttctctggt aaatcacttt gt 232 <210>

<211>

<212>
DNA

<213> fells Ctenocephalides <400>

gataagaacagtggactctcgtgctcttgcacttctctatgttgaataggagcaccgccc60 aatttgtcatcaagttctacagtcataatggctgcggcacctgcctcatcttgtgacgtt120 tccaatccaagccagaaatgaacatcccagctaaattggcccttcttgtcttctttagta180 ttcaggatgatgtatgagtctcctgtgtagaattttccaaaatcatttttaggatacgcg240 acgggttcaaaattctctacacgccacacctccaagcctttggtagta 288 <210> 171 <211> 500 <212> DNA
<213> Ctenocephalides fells <400>

gttaattcccattaacacgcggcgtttttcgtttagcaattcaataaattacacacttca 60 caatggctgatatggaagatactcatttcgaaactggagactctggagcttcagcgacct 120 atcccatgcaatgttctgcacttcgaaaaaatggttttgtaatgttaaaatcccgccccg 180 tgtaaaattgtagaaatgtccacttccaaaactggtaaacatggtcatgctaaagttcac 240 atggtcggaattgatattttcaacggcaagaaatatgaagatatatgcccatctactcac 300 aacatggatgtcccacatgtaaaacgtgaagattatcagctcactgacattgatgacggt 360 tacttaacattgatggctgacaatggagaccttcgagaagatcttaaaattccagatggc 420 gaattgggacagcaacttcgtaatgatttcgaatctggaaaggagcttttgtgcctgact 480 aaaatcttgtggagaagaat 500 <210> 172 <211> 500 <212> DNA
<213> Ctenocephalides fells <220>
<221> misc_feature <222> (5) . (5) <223> n = unknown at position 5 <400> 172 gccgntgaag ccgcaccagc tccagctgca gcccccgccg ccagcgaacg ccaatcatcc 60 aggggatccc gcaaagccgt caagcgcagt ggatctaacg ttttctccat gttctcacaa 120 aagcaggtag ctgaattcaa agaagccttc caactaattg accacgacaa agatggtatt 180 FC-8-C2-PCT.ST25.txt cattggaaagaacgatctccgtgccactttcgacgaattgggccgtttggtacaagagaa240 agaactcgacgacatgatcggtgaggcttcaggaccaatcaacttcacccaattgttgac300 cttgttcgccaaccgcatgtctggatcaggtggtactgatgatgatgatgttgtcatcaa360 cgccttcaagaccttcgacaacgacggcaaaatcgacagtgacaggttacgtcatgccct420 catgacctggggagataaattcactgccaagaagttgatgacgcctacgaccaaatggca480 ttgacgataaaggcttcatt ' 500 <210>

<211>

<212>
DNA

<213> fells Ctenocephalides <400>

gatgccaaaacagaaccgtgtcgcaatctacgagcacctctttaaggagggagttatggt60 ggccaagaaggactaccatgcaccaaaacacccagaattagagcaaattccaaacttgca120 agttattaaggctttgcagtcgttgaaatctagaggatatgttactgaacaatttgcatg180 gaggcatttctactggtatctgacaaatgaaggtattgaatacttgaggacatacttgca240 cttgcccccagagatcgtgccctccactctcaaacgtcaaaccaggcccgaattggcaag300 gccaagaccagctgccggcccaaggactgaaggatctcgtccagctgaagacagatctgc360 ctaccgtagggcacctggtgcacctggtggcgctgacaagaaggctgatgtcggtgctgg420 cactggagacttggaattcgtggtggatatggacgtggcagacctgccctcaataaattt480 atataagtaatttataataa , 500 <210> 174 <211> 500 <212> DNA
<213> Ctenocephalides fells <220>
<221> misc_feature <222> (4) . (6) <223> n = unknown at positions 4, 5 and 6 <220>
<221> misc_feature <222> (41) .(41) <223> n = unknown at position 41 <220>
<221> misc_feature <222> (61)..(61) <223> n = unknown at position 61 <220>
<221> misc_feature FC-8-C2-PCT.ST25.txt <222> (179)..(179) <223> n = unknown at position 179 <400>

aggnnncacgcctatgctcaaaagaacttgcgcagatctgntcgtcgcattaaggaactt 60 nccttccaagctgaagaagaccgcaagaaccacgaacgtatgcaagaccttgtagacaaa 120 ttacaacaaaagatcaagacttacaagaggcagatcgaagaagccgaagaaatcgccgnc 180 ctcaatcttgccaaattcgcaaggctcaacaagaattggaagaggctgaagaacgcgctg 240 acttggctgaacaagccgtcagcaaattccgcgcgaagggacgtggtggatccatggcac 300 gaggtggcagcccagtgcccgcaagaacagcagctcgcccacaatttgacggaatggctt 360 tcccgctaggttcgacttgaaccctgacagcgagttctaaattatttataattaatttta 420 aaaaagcgaacgtggctgcttgacagtaaacattaatattttttaaaaattattaaaagt 480 aaaaaatatcagctcgttca 500 <210> 175 <211> 500 <212> DNA
<213> Ctenocephalides felis <220>
<221> misc_feature <222> (489)..(489) <223> n = unknown at position 489 <400>

gtaaaaacaaaataaaatcattttagtttgagttagaacatgtgttattataagttagcg60 taacacacaaaaattaatcataaatgtctgataataattggtagtatacttgtacagtaa120 attctttattttccttttcccaatctttttctaaaagcgagtgtggtcatactgctgtgg180 taattcgtcaccgagaagcggatgaagtttatcctccatcattagtgaaggctttgcttg240 tcagaagagagaggagtgttggttgccggtgtttattgtagttttaatttaattttaaac300 taagttatgcgtatgacccaagacatggacgatgatgaaaagggaaagcttttcgtcgga360 gggctatcatgggagactactcaggagaacctgcagaggttcttctcgaggtacggcgaa420 gtgatcgattgcgtcgcatgaagaacagcgagtcggcaggtcgcgcggcttcggcttcgt480 cacatttgncgatccctcga ' 500 <210> 176 <211> 500 <212> DNA
<213> Ctenocephalides felis <400> 176 aagccgtggt agtttcgctg tatgaggaac ctattcagcc tcaacaagct gcttctgtcg 60 FC-8-C2-PCT.ST25.txt tggagaaacttggtgactacctcataacttgcggctactaaatgtgaaacactggagtgg120 ccccttattaatttttaagaaaactataataattataattacgattaatgaataaattta180 aacaatgaagaccggccagcaaaaagggacagaatttttttctcatgcctcctgcaatgc240 agtaaaatattttgtgccatttttgtgcctttgttcccggtgacctttttttcctcagaa300 gggacacctggagtgtactggcaaaatcgaggacattagtttaaacatttgtattataaa360 accgtgcggacggcaaaatgaataaaaaatcttactacgattaaatttgtattaatttcg420 atgtgaggttatcgacttgtggatttaaatatactttctcctttaataaaactcgtgctt480 ttgcaatttatgatttgcta 500 <210>

<211>

<212>
DNA

<213> felis Ctenocephalides <400>

atcaacttggattttgtcggttaattccgaaatcgaaaaagtttttaagtgcaaaacatt60 taactgtgacatcagtttgtaatgtatctgggaaggaaatgagatctgtaaatccgattg120 aaaggtttcctccttatgactataagaagaaaggatacggatttatcaatgcgttttttg180 attacacaactaagagattcaatgataacacaaaggttattacagtggaaggtccaccag240 ctgttggcaaaactgcatttgctaaagctttggccgaagatttagatatgaaatactttc300 cagctgttactatggatcactactatattaatgaatatgggtatgatctcagacaacttg360 atgataaattacctgaatcatgtaaaagcatcgattttgataaatttcataaagatccgc420 ataatagaaatgtagcaacaatgcaaattgtgttatatatgaaaaaatatgaacaatatc480 ttgcggctcttgccatttat 500 <210> 178 <211> 500 <212> DNA
<213> Ctenocephalides felis <220>
<221> misc_feature <222> (492)..(492) <223> n = unknown at position 492 <400>

gtcattcactttttacgagttctttagtgtcctaatttattaataagtatcagaatggtg 60 ccgaaaacaaaagtatttgttggaagcttgccgccaggctccaagcctgaagaattacgt 120 cgtttgttcgaagcttacggtgttgtaacagaatgtgatattatgaatcgttgtggcttt 180 gtacatatgcagaccgaagagatggcttttagcgcgattcaagcgttgaataatactaca 240 tttaatggggccacgataagtgtcgaaaggggccgcatcaaggaacgcggatcaggtggt 300 FC-8-C2-PCT.ST25.txt ggtcgtggag gcggtggccg cggcggtcga ggatttggag gacgaggagg tggtatgaat 360 cgtagtggtg gaggacctgg gggtatgcgt aatggtggtg gccccatggg aggtggcatg 420 cgtcgtggag gcggtggccc aggacccatg cgtggtggtg gccgtgacat gaatcgtgga 480 caccctattc tngtgatggt 500 <210>

<211>

<212>
DNA

<213> felis Ctenocephalides <400>

atttgaatcctcttatcgattactaatcttttgtacagtttaatatttattatcaaggcc60 ttgtacgccagtgtgtgtaaaggtctcttcagtcgacgacagaggatagaagtcagagga120 catttataatacgcagtttttaagttttttctggtgaatttgaatatatttgaagaaaat180 gaagaggtttttggttgctttggcggttttggtggtcgttgctgaggctaagctgtcatg240 ttcgagtacaaaagcctcgatccccgaagaatggatcgacatgacagcccagtgcacgag300 gagcatgaggaaccagatccaagaagaactgagcgcttcgatgcaatacttggccatggg360 ggcgcatttctcaagagacactgtcaacaggccaggatttgctgagatgttcttcaaatc420 ggcaagcgaagagagggaacatgccatgaaactcatgtcttacttgatgatgagaggaga480 actgccgagaggctgcagga 500 <210>

<211>

<212>
DNA

<213> felis Ctenocephalides <400>

aagaagatcaagaagaagaaggcaaaagaagagtctggagatgCCCCagCCg'CtgaagCC60 gcaccagctccagctgcagcccccgccgccagcgaacgccaatcatccaggggatcccgc120 aaagccgtcaagcgcagcggatctaacgttttctccatgttctcacaaaagcaggtagct180 gaattcaaagaagccttccagctaattgaccacgacaaagatggtatcattggaaagaac240 gatcttcgtgccactttcgacgaattgggccgtttggtacaagagaaagaactcgacgac300 atgatcggtgaggcctcaggaccaatcaacttcacccaattgttgaccttgttcgccaac360 cgcatgtctggatcaggtggtactgatgatgatgatgttgtcatcaacgccttcaagacc420 ttcgacaacgacggcaaaatcgacagtgacaggttacgtcatgccctcatgactggggag480 ataaattcactgccaagaag 500 <210> 181 <211> 500 <212> DNA

FC-8-C2-PCT.ST25.txt <213> Ctenocephalides felis <220>
<221> misc_feature <222> (5) . (6) <223> n = unknown at positions 5 and 6 <400>

acttnnctccatgcaagcctctcttgaagccgaagccaagggcaaggctgaggctttacg 60 catgaagaagaagttggaagccgacatcaacgagcttgaaattgctctggaccatgctaa 120 caaggctaacgccgaggcccagaagaacatcaagcgttaccaacaacaacttaaggatgt 180 acaaaccgccttgaggaagaacaacgtgcacgtgatgatgcccgtgaacaacttggcatt 240 tctgaacgccgtgccaatgctctccaaaacgaattggaagaatcccgtaccttattggag 300 caagccgacagaggccgccgccaagccgaacaagaattgggagatgctcacgagcaattg 360 aacgaactttccgcacaaaacgcttcagtatctgctgcaagaggaaattggaatccgaat 420 tgcaaaccttgcattctgacttggacgaactctcaatgaagccaagactcagaagaaaaa 480 gcaagaaggcatggttgacg 500 <210> 182 <211> 500 <212> DNA
<213> Ctenocephalides felis <220>
<221> misc_feature <222> (5) . (5) <223> n = unknown at position 5 <220>
<221> misc_feature <222> (34) . (34) <223> n = unknown at position 34 <400>

agacngctgtttttgtgttcaaaaagcagtaaantatagaaaaaaaaaacttattactag 60 tgcacattaacaaaaataagcagaacaactattaaaaatatgttttccatgtcaaacctt 120 ttctaaaaaccattcaatgcatagaaatgtaatttttaaaatcaatagtatgatttttta 180 aaatatttggttttataccaggagtggactactgaatttctgcagtgttgctatggctct 240 caatgatctagggtacaaagctgttggtatacgaatagactctggtgatttagcatatct 300 ctcagttctggccagagaaacatttgagaggatagcagagaaatacaacattccttggtt 360 tgcaagactcatgataattgcatcaaatgatatcaatgaagacacaatactcagtctcaa 420 tgaacagggacacaaaatagattgttttggaattgggacgcatttagttacatgtcaaag 480 gcaccagcacttgttgtgtc 500 FC-8-C2-PCT.ST25.txt <210> 183 <211> 500 <212> DNA
<213> Ctenocephalides felis <400>

gtggttcaaagataatccaagatgggtaagggaaataatatgatccctaatgggcacttc 60 cacaaagattggcaacgttttgtgaaaacttggtttaaccagcccgcaaggaaaattcga 120 agacgtcaaaacaggattaaaaaagctcgtgccttgttccctcgtcctgctgctgggccc 180 ctcagaccaattgtgcattgccccacagtgcgataccacactaaggttcgagctggcaga 240 ggttttaccctcgaagaaatcaggggagcaggtttgaatgcaggatttgctcgctcgatt 300 ggcattgcattagatgttagacgtcgtaataaatctgtagaatctttacaacagaatata 360 caaagattaaaggaatacagatctaaattgatcttgttcccaagaggtggaaagaaattg 420 cataagggtgaagcaactgaagaggaatgcaaggttgcatctcaattagaaggcgtcgtg 480 atgccaattaaacaaacttc 500 <210> 184 <211> 500 <212> DNA
<213> Ctenocephalides felis <220>
<221> misc_feature <222> (4) . (5) <223> n = unknown at positions 4 and 5 <400> 184 aatnntattctgaatactatgttcgattttagcgttttcccggcgagcttgatataatat 60 taattgtttggagtaaaaacaacaaattttcgaggattaatttatacaaaaaataattaa 120 aatgaaattagttaggtttcttatgaaattatcacacgaaacagtgtcgatagaattgaa 180 aaatggaactcaagtaaatggaacaataactggcgtggatgttgccatgaatacgcattt 240 gaaagctgtcaaaatgactattaaagatcgagatccagtatttcttgatactataagttt 300 gagagggaataatattaggtactatattctcccagatagtttaccattggagaccctttt 360 gatagatgatacacccaaagccaaagctagaaagaaggaagcagctcgaggtggaatacg 420 aggaaggggtcgtggtcgtggtggcccagaggcggtcgtggaggtggtaggggacgtgga 480 cgaagataataatatttttg 500 <210> 185 <211> 500 <212> DNA
<213> Ctenocephalides felis FC-8-C2-PCT.ST25.txt <220>
<221> misc_feature <222> (5). (5) <223> n = unknown at position 5 <400>

gaatntgctactttacaagccgctattgaagcaatcagttcaatgaacatgtttgaccta 60 ggaggccaatttttaagagtgggacgtgctataacgccgccgaacgctcttatgggaccg 120 acaactggatctatgatgccgactgctgctgctgttgccgcagctgccgccacagctaaa 180 attcaagcgatggatgcagttgctagtaatgctgtcgctttggggctgccgagtttgagt 240 tcttcacctacaattccgttatcagtgccgacgatagcggtgccgcccatcgcaacgata 300 acgccatcagctcccgttgctatatccggtgctgttaagttaccaggtgtcgtcataccg 360 cgcaggtgtcgtcgtacctcaagtcatacagcctccaggaattgtgacgccgacatcgca 420 accagttataattccagtatcagtattacctaattgtgaatcttctccgacgatgatatc 480 aacaagctcacctgcacaca 500 <210> 186 <211> 500 <212> DNA
<213> Ctenocephalides felis <220>
<221> misc_feature <222> (34)..(34) <223> n = unknown at position 34 <400>

atttttgccaaacacgatcaatctcaagotattntcaacagacacaaggatgttttagcc60 agatggcagcgtctcctgggagactcccgtgctcgcaagacccgtttgctggacatgcaa120 gaacagttcagacagatcgaggaattatatttgacattcgcaaagaaggcatcagctttc180 aattcctggttcgagaatgccgaagaagatctcacagatcctgtcaggtgcaactcgatt240 gaggaaatccgtgccctgagggaggctcatgcacagttccaggcttctttgtcgtctgca300 caggctgattttgaagccctggcagcocttgatcgccaaatcaagacgttcaatgttggc360 cgaatccttacacctggttcaccatggaggctctcgaagacacCtggaggaacttgcaga420 agataattgctgaacgtgatactgaacttgctaaagaagotcaacgacaagacgaaaatg480 ataaattgaggaaagagttt 500 <210> 187 <211> 500 <212> DNA
<213> Ctenocephalides felis <220>

FC-8-C2-PCT.ST25.txt <221> misc_feature <222> (5). (5) <223> n = unknown at position 5 <220>
<221> misc_feature <222> (473)..(473) <223> n = unknown at position 473 <400>

caggnttccacaacttgtcattaatatctcggtctcttcactgagacggtaaaagaattt60 caaaatgggttacttttattgtatatcacttttatgcctgttcactatagttagctgtaa120 taccggattagatcctaaaggtccaaaagttactcatgaggtatactttgacatcagcat180 tggtggtgaacctgcaggcccgtgttttgatcggccttttcgggggcactgtacccaaaa240 cagttgaaaatttcgtagaattgtccaagaaacctaaaggtgaaggatacaaaggcagta300 aattccacagggtcatcaaggacttcatgattcaaggtggtgacttcaccaggggagatg360 gaactggaggccgtcaatttatggagaacgttttgctgatgaaaactttaagttgaagca420 ttatggtgctggatggttgccatggcaaatgctggcaaagacctatggatctnaattttt480 atcctacaaaccctgctggt 500 <210> 188 <211> 549 <212> DNA
<213> Ctenocephalides felis <220>
<221> misc_feature <222> (5). (5) <223> n = unknown at position 5 <220>
<221> misc_feature <222> (525)..(525) <223> n = unknown at position 525 <400>

atgtnttcgggcatttagtgttttgaagtatcgtggcataaagcagatttaagttaatct60 gcttcattcaatacacaacatggtagggaaacccaacaagatgtatgtttttaagcgaga120 tggccgcaaggaagaaattcattttgataaaatcacttcaaggattcaaaagctatgtta180 cggattgaacatggattttgtagaccctgtctcaataacattaaaagtcataaatggatt240 atatactggagttacaactcaagaacttgataatctggccgcagaaactgctgctaccat300 gacaacaaatcatgctgattatgccgttcttgcagcacgaatagctgtttcaaacttgca360 caaagaaacaaagaaacaattttcagatgtcatggatgatttgtcaatatgactaatgag420 FC-8-C2-PCT.ST25.txt tatactaaaa agagatcacc aatgatagca gattatcatc ataaaataat aatggataat 480 gcagatcgca ttaactctgc tattatttat gatagagaat tcagntacaa ttactttggg 540 ttcagacat 549 <210> 189 <211> 549 <212> DNA
<213> Ctenocephalides felis <220>
<221> misc_feature <222> (532)..(532) <223> n = unknown at position 532 <400>
189 aaataaatttattataaaatgttagatctatttacaatatttagtaaagg60 gttatttgtg gggcatagtgctctggtgctttcagagcactagccacatatttgcaccatctgtaaatgc120 acttattagaagtgtcatattgcaggagcgatctggaaaaaatacctacgaccacgattc180 cttaacattgcaatataaattagacaatgaatttgagctggtgtttgtggttgcctttca240 gaaaattttgcaactgtcatacgttgataaatttttaaatgatgtacatttagagtttag300 agataagtataagaatgacttacaaaataaaaggtattttcaagaatttgactttggtgc360 ttgttataacagtattctgcgggcggctgaagaatggggtcggactcaggctaagttacc420 taaacaaatgcgttcttttgaagattctatgaaatctaaaaagacagttgcttcaatgat480 tgaaagaaagggcgagggaaaaaaagaccccagaagaatggtaaacaaaaangcaaaaat540 ggttggttt 549 <210> 190 <211> 387 <212> DNA
<213> Ctenocephalides felis <220>
<221> misc_feature <222> (386)..(387) <223> n = unknown at positions 386 and 387 <400>
190 gcacggtcggggacatgtgaaccctgtaagatgcacaaattgtgctcgat60 gacgttctaa gtgtccctaaagacaaggctatcaagaagttcgtcattaggaacatcgtcgaagcagctg120 ctgtacgtgatatcacggaggcttctgtttattccgcttacgtcttacctaagttgtacg180 ctaagttacactactgcgtttcctgcgctattcactctaaggtagttcgcaatcgctcta240 aggaagatcgtcgcattaggactccaccagtgagatcattcccaagagataatcaaaggc300 aacaaaatgctccaagaaagtaaatgttcatattttaaataaaaaaaacgacaacttaaa360 FC-8-C2-PCT.ST25.txt aaaaaaaaaa aaaaaaaaaa aaaaann 387 <210> 191 <211> 549 <212> DNA
<213> Ctenocephalides felis <220>
<221> misc_feature <222> (5) . (5) <223> n = unknown at position 5 <400>

attgnttaaattaaactgttgtcatttaagctttaaattacacactgacaacggagaagt 60 acagtttatttcgcaatcgttatgttaacgttttctcttacatgtatgaaagttatttag 120 ttataattaaaaatggctctgcaaagcttgaaaggtttttctaaattgttaaacaaatgt 180 tcgaatcgtgtttcatatttaactgtaagatgtgcatctcaatattatgacatagatgat 240 aacatatttggattaaatgaggatcaggttcagttgaggaagaccattttcgattttgcc 300 cagaaagaattagcacctaaagcagctgaaattgataaaaataatacctttaaagaactg 360 agagagttctggagaaaattaggagacttgggaacattaggaataactgctccagcagaa 420 tacggtggaacagaaggcacatatttagatcatgttattattatggaagaattatcaaga 480 gcttcgggagctattgccctctcatatggagctcattctaatctagcaataaatcaaatt 540 cgaaggaat 549 <210>

<211>

<212>
DNA

<213> felis Ctenocephalides <400>

gcttttaacgctcttataaaagaaattgttaaatctattgaaatccttattcatcggtgc 60 ctattttcggcttaaagatggttcaaaagaagcctaagaagaaggtcggaaagaaagtgg 120 cagccgcccctttggctgtgaagaagtctgaacccaaaaagttggttaatcctttgttcg 180 agaagaggacaaggaactttggaatcggtcaggatatccagccaaccagagacttatcca 240 gatgcgtgagatggcccaaatacatcagaatccaacgccaaaagagcgttttgcagaagc 300 gtttgaaggtccctccaccaatcaaccagttcacgcagactttggacaaacaaacagcta 360 cacagttgttcaagattttagaaaagtacaggccggagactgctattgctaaaaaggcac 420 gtttgcaagcacgtgctgaagctaaagcccaagggaaggaagacacacctacaaaacgtc 480 caaatgttgtacgttctggcacaaacactgtcacaaaacttgttgaacaaaagaaggcca 540 cttgttgtg 549 FC-8-C2-PCT.ST25.txt <210> 193 <211> 493 <212> DNA
<213> Ctenocephalides felis <220>
<221> misc_feature <222> (395)..(395) <223> n = unknown at position 395 <220>
<221> misc_feature <222> (407)..(407) <223> n = unknown at position 407 <400> 193 gctaatttaaaaaccatatacgcttttgcgcgagagatgcattccaaaaatataaataaa 60 cctatacaatatggtacagctggatttcggaccaaagctacagacttggactatgtcatg 120 tttcgtatgggggttcttgcagctcttcgttcccgtgttaaaagcacagcagtaattgga 180 ttaatgataactgcatcacataatcctgaagaagataatggtataaaactcgtagacccc 240 catggtgagatgcttgaacaaagttgggaagagttagcaaccactttagttaacgttagt 300 gattctgagttagaggctacagttgaaaaaattgtaaaagacttgaatatcgatcttaat 360 ttgaaggctaatgtgtttattggtatggatacaangtatagtacgtncccagtctttgca 420 aagctgctgttgatggagtattatctatttcgggggatctcccagagaattttggtattg 480 gtaccgactccca 493 <210> 194 <211> 482 <212> DNA
<213> Ctenocephalides felis <220>
<221> misc_feature <222> (7). (7) <223> n = unknown at position 7 <220>
<221> misc_feature <222> (36) . (36) <223> n = unknown at position 36 <400> 194 ttttttnatagtgatgcatcctttatcatt aggagntaatactaattatccaaacaattt60 gaacaagaatatttattggtttaatttcaa aaacattttgattttcatatattttattta120 tttcatttattggaggaatattaattctat ttatttatataacaagaatttcaaatactg180 aatatttttctttaaatatttataaaacta tttttattattttattattaacattaagaa240 FC-8-C2-PCT.ST25.txt ttattatttattataataaaaattttctattaatttttaacaataattccatatttaatg300 attttaattcaaacttacttatatataattttaatctaaataaattatataattttccaa360 ataatttattaactattatattaattatttatttattaatttcattaatttgtgtagtta420 aaattacagatattttttatggtcctttacgaaaaaatttttaaaaaaaaaaaaaaaaaa480 as 482 <210> 195 <211> 549 <212> DNA
<213> Ctenocephalides felis <220>
<221> misc_feature <222> (5) . (6) <223> n = unknown at positions 5 and 6 <400> 195 gttgnnacagatacgtagctgttaaatgtcattgcgttgctatttttgattatttcatca60 gtatttcaataaatccaacatatgtctcggcacacatcattataattaaattattagttt120 catttccattaatttattttactggcgtcaatatgacggtatcaaacgacgaattagttg180 aatcttttaaagctttaggtttaagtgaacaaaaggcgaaagaaacgttaaaaaatacag240 ttgttacgaaaaatttaacattagcattacatgaggtgaggggcattattttgccccaag300 gagctggttctttaatctattatgtggcaacaaaaatcaaaccacagattatagatcaat360 tgcctgtacttgtaaaatatatatcaacatcaaaattagacacaacagttagagttgatg420 cggccttgcaatttatgttgtctcatttaaatggatatcaaatcgatgaatttgagaagg480 cttgtggaattggtgttgttgtacacctgaacaaattgaaaaagcagtaaatgaggctat540 gttggaaca <210>

<211>

<212>
DNA

<213> felis Ctenocephalides <400>

ctttagtatattcggatataatttctatgttttatcaaatatattgttaaaaatgttata 60 gtgttattgtaaataattagtcattaataaaaaatggctacatatgaagaatttattcaa 120 caaaatgaagatcgagacgggattaggcttacatggaacgtttggccttctagtaggata 180 gaagccactagattagtagtcccgctagcatgtctttaccagcctttgaaagagaggcct 240 gatttaccaccaattcaatatgatcctgtacaatgtacgagaaatacatgccgagcaatt 300 ttaaatcctttatgccaagttgactatagagccaaattatgggtttgcaacttctgcttt 360 FC-8-C2-PCT.ST25.txt cagaggaatc cgtttcctcc acaatatgct gcaatttctg agcagcatca accagcagag 420 ttgattgcaa gtttctctac aatagagtac accatcacaa gggcaccatg tatgcctcca 480 atattttata tgtcatggga tacatgcatg gatgatgaag aactgggtgc tttaaaagct 540 ctttacaat 549 <210>

<211>

<212>
DNA

<213> felis Ctenocephalides <400>

tttttattcgttttacaaatcagtctcaaacgataaacacaaagtgaacgttttaaaaca60 aacataaaactgtgcctaatcccattcagtgatcgaaaaaactatataaaaccagttcga120 tagtaataatacataatttacccagcaacgtgcactgtataacctacaaatcacgatttc180 gactgaatgaaattatggacgctgaagatttgaatcagttctacaacgggcgggagatgg240 gtgcttggcctcccaatgttggcatccttgccatcgaactgacgtttccgtcgcaatatg300 tcgaccaggccgaactcgaggcattcgatggagtgtcagcaggaaaatacacaattggac360 tcggccaagcaagaatgggcttctgtggtgaccgagaagatattaattcattgtgtttaa420 cagttgttaaaaatttgatggaaagacatcaagtgccttatgatagaataggttatttgg480 gagtaggcacagaaacccttctcgacaaatcaaaaagtgtaaaatctgtccttatgcaat540 tattcgaac 549 <210> 198 <211> 549 <212> DNA
<213> Ctenocephalides felis <220>
<221> misc_feature <222> (6) . (6) <223> n = unknown at position 6 <400>

gttttntataaattaatatatcgttctcgttgttaccaaattatattatttaaattaagt60 gaaaaatatggccagtgttgtgaagcagttgccaagaatatccggctccaaagtagcgaa120 atgcatttcgagccgtaattattattcgtatgtaaatgagcctgcccaacaaatcaaaga180 caaagaaccgaaatgggttactgccgaagaagccgtaaaaattattaaatcagatgactg240 ggtgtacgctcagggtgcggctgctaccccaatcaacttattaggtgcgatgactgcaca300 tgggaagaagcaaggcctgaagaatgtgcgcgtctgccacatgcacactgaaggacctgc360 attatacgctcagcctgattgcgaagggatatttagatctgtatcattcttcatgggagg420 caacgtacgtgcagctgtgaatgaaggacgaggcgatgcaattccaatcttcctatcaga480 FC-8-C2-PCT.ST25.txt aatacctctt ctattccaaa agaaaattat tcaacctgat gtagccatag ttcatgtttc 540 tcctccaga 549 <210>

<211>

<212>
DNA

<213> felis Ctenocephalides <400>

gcttttcgatgttgatgccaaaacagaaccgtgtcgcaatctacgagcacctctttaagg60 agggagttatggtggccaagaaggactaccatgcaccaaaacacccagaattagagcaaa120 ttccaaacttgcaagttattaaggctttgcagtcgttgaaatctagaggatatgttactg180 aacaatttgcatggaggcatttctactggtatctgacaaatgaaggtattgaatacttga240 ggacatacttgcacttgcccccagagatcgtgccctccactctcaaacgtcaaaccaggc300 ccgaattggcaaggccaagaccagctgccggcccaaggactgaaggatctcgtccagctg360 aagacagatctgctaccgtagggcacctggtgcacctggtggcgctgacaagaaggctga420 tgtcggtgctggcactggagacttggaattccgtggtggatatggacgtggcagacctgc480 cctcaataaatttatataaagtaatttataataaaatatcaataaaacatcttattgata540 aacc 544 <210> 200 <211> 549 <212> DNA
<213> Ctenocephalides felis <220>
<221> misc_feature <222> (4). (4) <223> n = unknown at position 4 <220>
<221> misc_feature <222> (6). (6) <223> n = unknown at position 6 <400>

tgantnttcctgttattgcattcaccaacactgattctccattgaaatttgttgatattg60 caattccatgcaacactaagtccaaccattccgttggtttgatgtggtggctgttgtcca120 gagaagtcttgagacttcgtggatcaattccccgtgagaagacttgggatgtcgtagttg180 atttgttcttctaccgtgatccagaggaagtagagaaagaggatcttgttcacaaggaag240 ttgtggccaaagttgagactgctgttgctcatgatactgctgaagtatgggcaggagatg300 agccagccactcaattatggactgatgatgcacctgttgctgcagttccggctgtttacc360 FC-8-C2-PCT.ST25.txt ctgctgctgc cagccaagat tgggccgagc aagtgcaaga agaatgggct gccaacccta 420 ccccagctgc tggtcaaact acttggggaa gctccacaca agaatggtca taaatcaaat 480 gatacttttt gtattggata aatgaaataa atgaaaatta aaattaaaaa aaaaaaaaaa 540 aaaaaaaaa 549 <210> 201 <211> 549 <212> DNA
<213> Ctenocephalides felis <220>
<221> misc_feature <222> (4). (4) <223> n = unknown at position 4 <220>
<221> misc_feature <222> (6). (5) <223> n = unknown at position 6 <400>

catntntgctttgtggaaggaaagtctaaaataagaatataaactatgccgggtaccggg 60 acgttcaaattattcatcggaaatcttgacgaaaaaactcaagcgtcagatatacgtcct 120 ctgttcgagaaatacgggacggtcgtcgaatgcgatgttgtcaagaactttggttttgtt 180 catatggagaccgaacagcaaggtcgggatgcaattcagaacctaaacggctacgtaata 240 aacggcgaggccataaaatgcgaagccgccaagagccggagggcgccgtcaaccccgacg 300 acaaagatattcgtcggaaatttgacagataaaactcgcgcgccggaggttcgcgaactg 360 tttcaaaaattcggtacagtcgtcgaatgcgatatcgttcgtaactacggtttcgtgcac 420 ttggacgcgagcggtgacgtgaacgaggcgattcgggagctgaacggaatgatggtcgac 480 gggcaacccatgaaagtgcaagtgtccacgagtcgcgtgcgccagaagccgggcattggg 540 tgatcccga 549 <210> 202 <211> 549 <212> DNA
<213> Ctenocephalides felis <220>
<221> misc_feature <222> (5). (5) <223> n = unknown at position 5 <220>
<221> misc_feature <222> (174)..(174) <223> n = unknown at position 174 FC-8-C2-PCT.ST25.txt <220>
<221> misc_feature <222> (502)..(502) <223> n = unknown at position 502 <400>

atttnagccaaccagcaagcagaaacattgcagactcaattaaatctaatgtcccagcaa 60 cgagatgaacttttggccaagatgagtgacactgaagacaaatataatagacaagttgct 120 gctctaaccaatttgcaatgtgccttagaacaattccaaagagataaagatcangaaatc 180 caccaatgcacagaaaggataagaaaccaattggaattggaacgacaggaacaaactgct 240 ttaaggaacgaaatccaatccctaaaaacgcaacttagtgaacaacaacaaggcctgatg 300 gctgcaggaagacttgccagtcaacttgaatcctcacaaacaactgtacaaaatctcaga 360 caagaattgaaagagagccaagacaaatacgctgctctgaccgccaaagtggagtcttca 420 aaaaacaaccaagccgacaaaatcgagaagagcttgtaaaaacctaatgctcggtacatc 480 cagctggtcaacaaaacgacangcacaaatctaaaatatatagcagtctcgacttcacca 540 gtcggatgc <210> 203 <211> 1098 <212> DNA
<213> Ctenocephalides fells <220>
<221> misc_feature <222> (511)..(511) <223> n = unknown at position 511 <220>
<221> misc_feature <222> (1060)..(1060) <223> n = unknown at position 1060 <400>

tgtttatattccttcaaaatgtccgtacgtgtgcaattcgaaaacaacaatgaaatcggt60 gtttttagcaaactaacaaacgcttattgtctcgtagcgatcggcggatctgaaaacttt120 tatagtgtcttcgaggctgaattggcagagactattccggttgtccatgctagcattgca180 gggtgtaggatcattggcaggttgactgtggggaataaaaatggtttattggtgccagct240 tcgacaactgacacagagttgcaacatataaggaattctttaccagaaagtgtaaaaata300 caaagggttgaagaaagattatcagctttgggtaatgtgatagcttgtaatgattatgta360 gccctagtacatccagatcttgataaggaaactgaagaaatcctaacagatgtctaaatg420 ttgaagtattccgcaaacagttgcagtaatgtcctagtaggctcatacacagttttaagt480 FC-8-C2-PCT.ST25.txt aaccaagggggcttagtgcccccaaaacatncatacaagacaagacgagcttcgtcatac540 tcaagtccgtgtttatattccttcaaaatgtccgtacgtgtgcaattcgaaaacaacaat600 gaaatcggtgtttttagcaaactaacaaacgcttattgtctcgtagcgatcggcggatct660 gaaaacttttatagtgtcttcgaggctgaattggcagagactattccggttgtccatgct720 agcattgcagggtgtaggatcattggcaggttgactgtggggaataaaaatggtttattg780 gtgccagcttcgacaactgacacagagttgcaacatataaggaattctttaccagaaagt840 gtaaaaatacaaagggttgaagaaagattatcagctttgggtaatgtgatagcttgtaat900 gattatgtagccctagtacatccagatcttgataaggaaactgaagaaatcctaacagat960 gtctaaatgttgaagtattccgcaaacagttgcagtaatgtcctagtaggctcatacaca1020 gttttaagtaaccaagggggcttagtgcccccaaaacatncatacaagacaagacgagct1080 tcgtcatactcaagtccg 1098 <210> 204 <211> 545 <212> DNA
<213> Ctenocephalides fells <220>
<221> misc_feature <222> (4). (5) <223> n = unknown at positions 4 and 5 <400>

gacnncggtgatgacagaggccgggatggcgattggacatgtcctagttgttccaacacc60 aactttgcttggagaaatgcctgcaacaggtgtagtgaagaaagacctgatggtgcaggc120 ggcggtgactccggccgaggtggtggccgtggaggcggccgcggcggtggtggtggaggt180 ggtagttacggtaaccgcggaggtggtgatagagattctggtcgcggtggtgatagaggt240 tttcgcggaggtggtggccgcggtggcggtggcggttatggaggtggccgcggaggcggt300 ggtgatcgtaattctggaggaggtggctcaatgcgtggaggagatagaggacgagatagg360 caacgaccttattaaataattagcttgtaattttattcaccctcattttcacatcattcg420 tactcatattagatttattgatttttagaattatttatatgatttgtatgattcaatgta480 acacaatattgtaaatcttatattaagatttaataatataaaataatagaaaaaaaaaaa540 aaaaa 545 <210> 205 <211> 532 <212> DNA
<213> Ctenocephalides fells <220>

FC-8-C2-PCT.ST25.txt <221> misc_feature <222> (3). (4) <223> n = unknown at positions 3 and 4 <400>

cannatgccacgaggaaaatataccaatcacaaagggcgtaacaggcatttcacaaatcc60 agaagagttagaggaacagagaaagcaagaagaacaaaaaaggcaatggcgtagggatca120 tggaaacgaatcaagttctgaggaagaggaagtagccaaaaaagctgcaggtgataagaa180 gaaagccccagggctggaaagctctgattcagagagtgaatcggagactgaatcttcaga240 agatgagaaggataagaagaaaggtgtatctggattgatagaagtggaaaatcctaatcg300 tgttcaaaaaaaaaccaagaaactttctactttaaatgaaacattaactgatagcaaacc360 acaattgtcaagacgagaaagagaagaagttgagaagcagagagcgcaagcacattacca420 aaagcttcatgcagaagcaaaactgtcaagcaagatctgtttagcaagacttgcatcatt480 aaacacagcgtgaagaacagcttaaagaccgagttggaaaaaaaaaaaaaas 532 <210> 206 <211> 454 <212> DNA
<213> Ctenocephalides felis <220>
<221> misc_feature <222> (5) . (5) <223> n = unknown at position 5 <400>

atttntgttggttaactcgagcagtaagaatatataaaaatggctgcagttggcaaagat60 ttagaaaaaccaacagctgaggtgcctcaggtccatcgcatccgtataactttaacttca120 agaaatgtacgttcattggagaaagtatgtaccgatttaataaatggagccaagaaacaa180 aagctccgtgtgaagggaccagttcgcatgccaaccaaaatcttgcgtattaccacacgt240 aaaacaccttgtggtgaaggttcaaaaacttgggatcgcttccaaatgagaatccacaaa300 agagtcatcgatctgcactctccatctgaaattgtgaagcaaatcacttccatcagtatt360 gagccaggtgtagaggttgaagtaacaatcgctgatgcttaaattttgtaaattgatgga420 aataaatgacaaaacctaaaaaaaaaaaaaaaaa 454 <210> 207 <211> 549 <212> DNA
<213> Ctenocephalides felis <220>
<221> misc_feature <222> (470)..(470) <223> n = unknown at position 470 FC-8-C2-PCT.ST25.txt <400>

cactttgaggttttgatccttaaccatggtgaacggacgcatcccgtccgtcttttcgaa 60 gacatatgtaacccctcgtcgtccttatgaaaaggctcgtttggaccaagaattgaaaat 120 Cattggagaatatggtctccgtaacaagagggaagtatggcgtgtcaaatacactttggc 180 taaaatccgtaaagctgcccgtgagttgctcacattggacgaaaaggatggcaaacgtct 240 cttcgaaggtaatgctttattgcgtcgtttagtacgtattggagtgttggatgaatccag 300 aatgaagctcgattacgtgttgggtttgaaaatagaagatttcttggaacgtcgtctgca 360 aacccaagttttcaaactgggattagccaaatcattcaccacgctcgtgttttgatccgc 420 agagacacattcgtgttcgcaagcaagttgtaatattccctcttcattgngcgttggatt 480 acaaaaacacattgacttctccctcaaatcgccttcggtggtggtcgtcaggacgttaaa 540 gaggaagac <210>

<211>

<212>
DNA

<213> felis Ctenocephalides <400>

cggtaataagggttgtgatcaacgaatcaatgacgctgctcaatttaaggctgtacgagc 60 agccatgtcagtcatcgaaatttctgaagaagaacaaatacaaatccttcgtgtcgtcgc 120 agcagttttgcatttaggcaatgttggatttactgaaagcgaaggaaaagcagaaatttt 180 gaaacctgaagctgtgcaatacgccagcgagttattgggatgttcatgtgaagctttatc 240 caaagcacttacacatcgaactatcgaaccaggccgagggttggcaaaggtcagttctcc 300 tttatcgcgagaaatggcaattcatgcaagagatgctttagcaaaggctgtttatgatag 360 aatgttcacttggcttgtgtCcaggctgaacgcttctttgcaaccggtcaaaaccaggag 420 ctattcggtatgggaatattggacatttacggatttgagatttccagaaaaatagttttg 480 aacaatttgataaacttttgCaatgaaaaacttcacactcttatcgagctgcttgaaata 540 gacaagaca 549 <210> 209 <211> 549 <212> DNA
<213> Ctenocephalides felis <220>
<221> misc_feature <222> (34) .(34) <223> n = unknown at position 34 <220>

FC-8-C2-PCT.ST25.txt <221> misc_feature <222> (525)..(525) <223> n = unknown at position 525 <400>

attttaataacatgacaaaaaattaaccctatantttgtttaagttattgtattaatata60 aattatttttatttaatttcattattaagaattataattggagctttaggtggattaaat120 aattcctctttacaaaaaattattacttattcttcaattacacatattggctgaataatt180 attgctttaataaataatgaaataaatttttgattttatttttttatttactttatcatt240 tctttaattattacaataaattttaattttttaaaattattttatataaatcaaatatta300 tctaattcaataaattatataacaaaaattactttaataataatatttttatcaatagga360 ggtttacctccatttttaggattttttcctaaatgaattattattgaatctataattaat420 ataaatataattttttattctattattataattttatgtactttattaatattatatatt480 attacgatttgcttttctatttaatataaataattccaaattctngaaaattatataaaa540 taaattttc 549 <210> 210 <211> 549 <212> DNA
<213> Ctenocephalides felis <220>
<221> misc_feature <222> (4). (4) <223> n = unknown at position 4 <220>
<221> misc_feature <222> (543)..(543) <223> n = unknown at position 543 <400>

cttntaaaaattattaataattccttaattgatcttccttctccatcaaatatttcatta 60 ttatgaaattttggttctttattaggactatgtttaattattcaattaattacaggatta 120 tttttagcaatgcattactgtgctaatattgatatcgcattttcatcagttagacatatt 180 tgtcgaaatgtaaaattatggttgattaattcgtaccattcatgcaaatggggcatcatt 240 tttctttatttgtatttacattcatattggacgaggaatttattattctagttacaaatt 300 acatgaaacttgatttattgggttattaattttatttttagtaataggaactgcttttat 360 aggatatgtattaccttgaggacaaatatcattttgaggagcaactgttattacaaactt 420 aatttctgctattccatatattggaaattcaattgtcaatgattatgaggaggatttgca 480 gtgataatgctctttaactcgattctttatgatcatttttattccatttattattctgtt 540 FC-8-C2-PCT.ST25.txt tgnaataat 549 <210> 211 <211> 549 <212> DNA
<213> Ctenocephalides felis <220>
<221> misc_feature <222> (4). (5) <223> n = unknown at positions 4 and 5 <220>
<221> misc_feature <222> (34) .(34) <223> n = unknown at position 34 <400>

gacnntataacaaaccgagattatttttgaaatnaaaggtattaggaaaaattattgata60 ataacataaaagtctaaatgcttttataatatgacagcaatgcgtactgcaatgaatgtt120 gtgtatacagttttgtggattctattaatttcacaaggtacccaaagcgttgaattaact180 tttgaactaccggataatgcaaaagaatgcttttaccaagatattcaaaaaaatacaagc240 gtcaccttagagtttcaggtcgtcacgggcggtcagtatgatgttgatgtaacattagaa300 agcccaaataagcaaattatatatagtcaagtgaaaacccaatttgattcgcatcatttc360 actgcaccgataagtggtgtttacgttgcttgtttcagtaatgaattttccacgttctca420 cacaaattggttatatggactttcaagttggtgatgaacagctttacccggtgtcgggga480 gcatgctacagtcttacccaacttgaatctttgccaagagattcatcgaagtttgccagt540 ttctcaatt 549 <210> 212 <211> 439 <212> DNA
<213> Ctenocephalides felis <220>
<221> misc_feature <222> (5) . (5) <223> n = unknown at position 5 <400> 212 aaatncgttacgtggctgcgtatttgttggctgttttgggaggcaaagccgccccgacct60 ccggagacatcgagaaaatcctgagctctgttggtattgaagccgatgctgaacgtttaa120 agaaagtcataagtgagctcaatggcaagagcatcgatgaactcattgaacaaggtcggg180 agaagctttcctccatgccagtaggtggtggtggtgcaccagctgcagctgctgctggcg240 gcgcccctgcggctgctgaagaaaagaaagaagagaaaaaagaggccaaaaaggaagaat300 FC-8-C2-PCT.ST25.txt cagagtctga ggacgatatg ggtttcggtc tctttgacta aggaatacgt atgccttacg 360 tataatgaaa ttataaattt aaatgtaaca tatgcataag aaataaagtg atttcattta 420 taaaaaaaaa aaaaaaaaa 439 <210>

<211>

<212>
DNA

<213> felis Ctenocephalides <400>

gtttaagtgtattcaaatcaaataattccataaaactattctagaaaataatccaacaca 60 gttagataagatgaagtctttggtgctcttggccgttttggctgtaggttttgcaaatgc 120 tgaatattgttacgatgaaagtgttactgcttgttcatcgactgccaataaggaggatct 180 ccctcattgtaatgccctctactccggtttccacactgtggctgccgacttatcatcata 240 cgttaaaagagaagtcctatactcctacgactacttgctgatgtctacacactttggaaa 300 ctatgaaaagaatcgtgttggtttcgaaaaactcttcaaaggcctctccgacaagtcttg 360 ggaaaacgcaatcaatgtcatcaaatacatcaccaaacgtgggggcactgtcgacttcca 420 gactactcacaatgtcaacactactcgtgtagctgaaacatcagaattggaagttgccag 480 ggctttggagaatgagaaagtgctcgtaaggatgtcaggctattcacaaacgtgtatcaa 540 ctccgacac 549 <210>

<211>

<212>
DNA

<213> felis Ctenocephalides <400>

attttagaatataaagctttaattatatttttaattttatatttttgttttaattatata 60 atatttgagaattatttttgtaatttttatattgtatttacagtatgtgaaggggtttta 120 ggcctttctattttagttgcaataattcgtactcatggtaatgattattttaaaagattt 180 aatataatacaatgttaaaatttttaataataatattttttataatccctattattttat 240 taaaaaattgttattgattggttcaaacaatattatttataatattatttatttatataa 300 ttttaggaataaaaagaattataattgttaatattagattaaattttggttatgatatta 360 tatcttatagattgaatttattaaggatttgaattattattttaatattaatagcatcaa 420 ttttaatttataaaaataattataattaaattatttttattattattaattttttattat 480 taatattatttttaacttttagtcattaaattattttgattttattttttttgaagaaga 540 taattccgcc 550 <210> 215 FC-8-C2-PCT.ST25.txt <211> 550 <212> DNA
<213> Ctenocephalides felis <400> 215 ctattcggaa aacttttcct gccgtagggc ccaaacctcc gctaatttct agtgagtgac 60 taaatttaat gtcgtggtaa gtggtgcaac aatgcagatt ttcgtcaaaa ccctcacggg 120 taaaaccata accctagagg ttgaagcttc tgataccatc gaaaatgtta aagctaagat 180 tcaagataaa gaaggaattc ctccagatca gcaaaggtta atctttgcag gtaaacaatt 240 agaggatggc agaacattgt cagactataa cattcaaaaa gaatccacct tgcatttggt 300 gctccgcctt cgtggtggtg ccaagaaacg caagaagaag aattattcaa caccaaaaaa 360 aatcaaacat aagaagaaga aggttaaact agctatttta aaatgttaca aggttgatga 420 aaatggtaaa atatttcgca tgaggcgtga atgccccgga gaggctgtgg agcaggagta 480 tttatggctg tatgacagat aggcattctg tggaaggtgg atcacttatt tcagcaaacc 540 agaagatatt 550 <210>

<211>

<212>
DNA

<213> felis Ctenocephalides <400>

tagcacctcaaaacctgcttcggctaagagctcaacaactaaaccagctgctgccaaacc 60 tgcagctaagcctgctgctaaaccagcagctggtaagccaaaagctccagcaaaatcttt 120 gtctaagccaacaagtgctcctaagaaagttactaaagttgcggctaagcccaaagttgc 180 tgccaaaccaaaacgcaatgtttctgttaaaggacagaaaggagccaagcctgcagctaa 240 agcagcagtcaagaaagctttaagtgtgcaaaagaaggtaattaaaggtgtaaatggcac 300 acgcacaaggaaaatccgtacctctgtacactttaggaggcccaagaccctcaggccacc 360 aagaaatcccaaatccccaggaagagtgtaccatctagaactcgcatggtgcttacaata 420 ttatcaaattccattgccactgagcagcatgaaaaaaattgagatccaatacttagtgt 479 <210> 217 <211> 420 <212> DNA
<213> Ctenocephalides felis <220>
<221> misc_feature <222> (386)..(386) <223> n = unknown at position 386 <220>
<221> misc_feature <222> (404)..(404) FC-8-C2-PCT.ST25.txt <223> n = unknown at position 404 <400>

cacaaatagggattaaaaaatggctccaagaaaaaacaaagtagcaaaagaagaagtgca60 ggtggctttgggacctcaagttcgtgaaggtgaaattgtttttggagtcgctcacatttt120 tgccagcttcaatgatactttcgttcatgtcaccgatttgtctggacgtgaaactattgc180 cagggtcactggaggcatgaaagtaaaggctgatcgtgatgaagcctctccttatgctgc240 tatgttggccgctcaggatgtggctgagaaatgcaagtccctcggcatcacagccctgca300 catcaagctccgtgccaccggtggaaacaaaaccaagaccccaggaccaggtgctcaatc360 cgctttgagagctttggccgttcttngatgaaaaatggacgcantgaagatgtgacaccc420 <210> 218 <211> 549 <212> DNA
<213> Ctenocephalides felis <220>
<221> misc_feature <222> (502)..(502) <223> n = unknown at position 502 <220>
<221> misc_feature <222> (541)..(541) <223> n = unknown at position 541 <400>

gcagagtaggcaaagtgtgactggcacttgtcagcgtttgtcgttcccgagtgctagatt 60 aggttatttaggattgttggccgatattccatcatctgggtaggctaaaatcaaggatcg 120 gaaagacgatcgagacgaacaaccatacaaaaatattttttagaacaaaatcaataaaaa 180 tttcaaaatgttatctcggattctaaaacctcttttaaatggatctactagagcgttttc 240 gactacaaacaagaataatgtcaaggttgcggttttgggagccgctggtggaattgggca 300 accccttagtcttttattgaaacagtcccctttgatcagtcgcctgaatttgtatgatat 360 ccttggaacgattggtgtggcagctgacttaagtcacattgaaacacctgccaaagtatg 420 tggttacaatggcaagaagttttgtgcaaggcttagaaggagtggatgtagtcgcatcca 480 gcagcagccctcgtaaacctgnatgactcgtgatgattggttgcatcatgcttcattgtg 540 ntgaataat 549 <210> 219 <211> 549 <212> DNA
<213> Ctenocephalides felis FC-8-C2-PCT. ST25 . tact <220>
<221> misc_feature <222> (444)..(444) <223> n = unknown at position 444 <220>
<221> misc_feature <222> (452)..(452) <223> n = unknown at position 452 <220>
<221> misc_feature <222> (521)..(521) <223> n = unknown at position 521 <220>
<221> misc_feature <222> (542)..(542) <223> n = unknown at position 542 <400>

gcgacctgtacacgcggtgaCaactattctgtgttcaatcgaaagttaacgcgcgtttaa60 caaaagtttttaaaaaaatccaataaggataactataaaataacgttttcagtgattcag120 ttataaatattcgtttgtgcatttcatatcattatggctaacaacagagctgcaaaatcc180 ggtttcgccgctgaggcacaaagaaagataaatagcaaatacagcgatgaacttgcacaa240 gaatgcttggaatgggtcaaaaccattactggagagcccgaaaatgttagcggcgatatg300 gacaatttttacgaagtattgaaggacggcacactgttgtgcaaattagtgaactctcta360 caagctggttctgtaaagaaagtcaatgagagcaaaatggcttttaaatgcatggagaat420 ataaatgctttcttggaagcggcnaagctatnggcgttcctgcgcaagaaactttcaaac480 ggtgatctgtggaaagacaaaatcttaattctgtgcatttnctcaatcattagggcgaaa540 gntggaata 549 <210> 220 <211> 301 <212> DNA
<213> Ctenocephalides felis <220>
<221> misc_feature <222> (32) .(32) <223> n = unknown at position 32 <220>
<221> misc_feature <222> (68) .(68) <223> n = unknown at position 68 FC-8-C2-PCT.ST25.txt <220>
<221> misc_feature <222> (70) .(70) <223> n = unknown at position 70 <220>
<221> misc_feature <222> (84) .(84) <223> n = unknown at position 84 <220>
<221> misc_feature <222> (88) .(88) <223> n = unknown at position 88 <220>
<221> misc_feature <222> (226)..(226) <223> n = unknown at position 226 <220>
<221> misc_feature <222> (277)..(277) <223> n = unknown at position 277 <400> 220 ctcatttattatttatttatttatattagttntgtgaggattatttgatatcaatcaata60 gttattgntnggagaaatggcgtncacnacgaaaacttcgaagtattcttacagatcgac120 cggcggaggaaacgctgatgtcagcatcgagtacagtgctgatctcagtgccctcaccag180 attggaggataaaattcgtcttttacacgatgacctcgaatccganagggaactccgtgc240 caggatcgagcgcgagaaggcagatttgagtgtacangtcattcaactgtcagaaaggtt300 a 301 <210> 221 <211> 549 <212> DNA
<213> Ctenocephalides felis <220>
<221> misc_feature <222> (374)..(374) <223> n = unknown at position 374 <220>
<221> misc_feature <222> (402)..(402) <223> n = unknown at position 402 <220>
<221> mist feature FC-8-C2-PCT.ST25.txt <222> (417)..(417) <223> n = unknown at position 417 <220>
<221> misc_feature <222> (440)..(440) <223> n = unknown at position 440 <220>
<221> misc_feature <222> (443)..(443) <223> n = unknown at position 443 <220>
<221> misc_feature <222> (445)..(445) <223> n = unknown at position 445 <220>
<221> misc_feature <222> (532)..(532) <223> n = unknown at position 532 <400>

attttcactggatacaaacgaggtctcaggaatcagcatgaaaacacagctctcttgaag60 gttgaaggttgtggtaccaaagccgatagctggttctatgttggtaaacgatgtgtattt120 gtgtacaaagctaagaacaagacctgcgtacccggcaaaccaaagaagggtaaatcaaaa180 agtacgcgctatttggggcaaagtaacacggccacatggatgctctggttgtgttcgtgc240 taagttccacagaaaccttccagcaaaagcaatgggtcatcgtgttcgtataatgctgtt300 cccatccagaatataaggcataggattcatcttttgttataaattataaaataaacccta360 taaaaaaaattaanaaaaaaaaaaaaaaaaaaaaaaaacctngggggggggcccggnccc420 aattcgccctatagggagtngtntncaattcactgccgtcgttttacacgtcgtgactgg480 gaaaccctgcgttcccaacttaatcgccttgagacatccctttcccagctgngtaataac540 aaaaggccc <210> 222 <211> 549 <212> DNA
<213> Ctenocephalides felis <400> 222 gcataccgac acctccattt ggtgtggcaa aatcatcagc tgaagcagct aaaatagcta 60 cggatttgaa aacaaatgac attgtactca aagcacaagt tttggctggt ggtagaggga 120 aaggccaatt caaatcgggc ttgaaaggcg gcgttcgaat ggttacaacg ccagagcaag 180 cagaaaaaat atcttcaaaa atgatcaacc aaatattagt aactaagcaa acaggtgaaa 240 FC-8-C2-PCT.ST25.txt agggtcggatatgtaattctgtcatggtcacagaaagaaagtttccccgacgagaatact300 atttggccgttatgatggagcgagctttttctggccctgtaattattgcttcatcacaag360 gaggagttaatatcgaagaagtagccgctgaagatccagaagcaataatttatgaaccaa420 ttgcataatgaagggaattacacatgaacagacaaaaaaattgttggcaattgacttgaa480 gatcatgcagaattaccgcagaatttagagaaatgtatgattggttttaaagaaagatcc540 tctattgaa <210>

<211>

<212>
DNA

<213> s fells Ctenocephalide <400>

gcgctattgagtcgcacgaataatttaacaaattatatatttaagtggaaattagataaa60 ttaataggacaaaacgtagcagtaatttcatgctcttactccacaaaaggatcaaaagtg120 tatgaagatgcttcagaagctgttaaagatataccaaatggagcaaaattactagttggt180 ggttttggtttatgtggaattccagagaatttaatcgctgctgtattaaaacatggtgtt240 tctggattaactgttgtttccaacaatgcgggtgttgatacgtttggtctgggattactg300 ttaaaagaaaaaaggatcaaacgcatgattgcttcatacgtgggtgaaaatgctgaattt360 gaaagacaatatttaagtggtgaacttgaagtagaattgactcctcaaggaacattagca420 gaaagaatccgagcaggaggagctggtattcctgttttttcacaccaaccgttatggacc480 cttgtcatgaaggaggcgctccattaaatatggaaagaaaggagaggtagaaattagagt540 cacctagct <210> 224 <211> 549 <212> DNA
<213> Ctenocephalides fells <220>
<221> misc_feature <222> (538)..(538) <223> n = unknown at position 538 <220> ' <221> misc_feature <222> (543)..(543) <223> n = unknown at position 543 <400> 224 aaagtctttt atcaatagtc ttttataaag cactttctac caggatacaa tttaaattac 60 attctataaa taaaaaagct tactattaaa atgaacataa ataccagaat gccataagtg 120 FC-8-C2-PCT.
ST25 .
tact tcttttctctcttatatacataataacatttcaataaatatttgtataaaaataatatct180 gaaatatgtacataactaaattaataggcaaatctcatttcaggattatatcctaattta240 tccagactaggctggcatgcaaatttgatcattggtggaggaccacacataagcactaga300 gtatcatcggaaggaggaaacaaatgttctgcaatcatgtctgagtttataaaaccagtg360 ctatatttccaattgtctggtgggctatcaagagtataccataatttaaattgagttgga420 tcatttttagcaatttcttccaatctatcacgcctaatatatccttttca~gtctggttgc480 aatatcaaagcgattcagttgatctggggttttacaattcctcactaactgagcatgngt540 aantctgac <210>

<211>

<212>
DNA

<213> fells Ctenocephalides <400>
225 gtttgaagtgtcagtgatataataataatcctaaagtgcaatgacatttt 60 gccattttca tttatataagagatgtttcttacaaagttaaatttcaagttattctctcccaataacttt 120 accttagcgataataaaaaaatattcgtgtttaacgtgccaaagtctaaccctactttcc 180 tctagctttacaaagcaagatgaatatagtttattaccaattagaaattattcacaaaat 240 actaaaaacgaattagctcattgcaacattggcactataggtcatgtagatcatggcaaa 300 acaacattgacagcagcaataacaaaaattttatcaaaaaatggttctgcaaaatacata 360 tcttatgatgaaattgatcgagctcctgaggaaaaagccagagggattccatcaatgcag 420 ctcatgttgggtatagcactactaagagacattatgctcatacagactgccaggtcatgc 480 cgatttgtaaaaacatgatatcagggcttacaaatggtggaccatttagagtggtgcaac 540 agaaggcaa <210>

<211>

<212>
DNA

<213> fells Ctenocephalides <400>
226 ctccaaaggatgcctccaaaaatcctatgagggaccttcacattaggaag60 gaaaaaaagg ttgtgccttaatatttgtgtgggagaatctggtgacaggctcactcgtgcagcaaaggtg120 ttggaacagttaacaggtcaacagcctgttttctcaaaagctcgttataccgtcagatct180 ttcggtatccgtcgtaatgaaaaaattgctgtccattgtacagtccgtggagctaaggcc240 gaagaaattttggaacgaggtcttaaagtacgagaatatgagttgaggcgagacaacttc300 tctggaactggaaactttggtttcggtattcaagaacacatcgatcttggaattaaatac360 gatccatccattggtatttatggtttggacttctacgttgtactcggacgaccaggtttc420 FC-8-C2-PCT.ST25.txt aatgttgcca cagaagcgca agactggcaa agtggtttcc aacccgttgc gaaagaagat 480 gctatgaaat ggtccaacag aaatatgatg gtatatttta tagtaaaagt gagaagacat 540 tttctgtat 549 <210>

<211>

<212>
DNA

<213> felis Ctenocephalides <400>

ctaaaccgaggcttcaaaactacgaaaataccttctggtaagccaggcggcgaaaagaaa 60 ttcaaagtacgccccgctcgcgtaaagggaatccaaacaaaacataccaaatttgtccgt 120 gatttggtaagagaagtcgttggacatgccccttacgagaaaagagctatggaattgtta 180 aaggtttcaaaggataagagggccctcaaatttttgaaaagacgcttgggaacacatatc 240 agagctaagaggaagcgtgaagaactttctaacatcttaacccaaatgcgtaaagcacat 300 gctgctgctacacatactaagtagatatgaataaagctgtttaattgtaaaaaaaaaaaa 360 aaaaa 365 <210> 228 <211> 549 <212> DNA
<213> Ctenocephalides felis <400>

ggacaatgaagcaaatcgtaacaaatcaatctgtgaagattccagaaggccttacggtca 60 ctgtgtcatcgagacaggtcactgtcaaggggcccaggggagtattaaaacgctccttca 120 aacatttagcagttgatattcgtatggtcaatcctagattattgaaagttgagaaatggt 180 ttggatcaaaaaaagctattgctgcagttaggactgtttgctctcacatcgaaaacatgt 240 tgaaaggtgtaactaaaggttatcaatacaaaatgcgtgccgtatatgctcactttccca 300 ttaattgtgtgaccacggaaaacaacacagttatcgaaatccgtaatttcttgggagaga 360 aatacatcagacgggtaaagatggcccctggtgttacagtgatgaactcccccaaacaaa 420 aggacgagctgatcttggaaggaaactcttggaagatgtatctaaatcagctgcattaat 480 ccacagtcgccctgtaaaaacaaggatatcagaagtcttggatggctttatgtgttgaaa 540 actcagtgt 549 <210> 229 <211> 549 <212> DNA
<213> Ctenocephalides felis <220>
<221> mist feature FC-8-C2-PCT.ST25.txt <222> (548)..(548) <223> n = unknown at position 548 <400>

gtctacattattttgtttcagtacgggttgaccaatcgctcatcaacgacgataaataaa 60 acactattaactgcaaaacgcaagttgacgaatattttttaatttaaacttataaatagc 120 atccactgaaatcattttgaataaatatttcacgatgtctatcaacgactgtgaagagaa~180 acgagaaatcatcgaaaggaagttggaaattccggatttcgcgttgaagcatccccttca 240 gcacacatggactttgtggtattttgaaaacgaccggcaaaagacatgggaagaaaatca 300 acgagaaatagtcagttttgctacaattgaagacttctggagtgtttatagccatataaa 360 gatggctagcgaattgcgtcaaggatgcgattattccttgttcaaaagcggcatcaggcc 420 aatgtgggaagatgacgccaataagcatggaggaagtgggccatcaccatggcaaaaagc 480 agcgacaaatgatttggatagaattggtggcaccctctctgttaatcgtgaacttttgaa 540 attccacna 549 <210>

<211>

<212>
DNA

<213>
Ctenocephalides fells <400>

cgctgaaatcatctcgcgataacgttttgaatgtgttttatatgaaaacagtggctattt 60 atttactcgatatgtgtccacagtaaagtatttgtgatttattcaagtgttgtgtgctga 120 tttagtttgttcaatagaacctaaaaaagaacaaggtttgtggtgtttgtgcttacaacg 180 ttttaggattttgaattcgtgactctacgtggataacgctagacttccaggcatagcaca 240 atgccggaagagaccaaagtggaggcggatgcacctcatagtcctgccaagcaaaaacgg 300 tcatcaggtggaagaactgttttggctaaagtcgacctactagatggatcagttcttgat 360 ctccatattgaacgtaaagtgaaagcaaaggagctcctggataaaatatgtgaagcttta 420 aatattttggaaaaagatttctttggcttatctcagtcaccatctgatccagagtctggc 480 tggatccagaaagacagtgctaagttcttaaatctgaccatgggtgtgcatcgagtaaat 540 ctcccccag 549 <210> 231 <211> 549 <212> DNA
<213> Ctenocephalides fells <220>
<221> misc_feature <222> (33) .(33) <223> n = unknown at position 33 FC-8-C2-PCT. ST25 . tact <220>
<221> misc_feature <222> (189)..(189) <223> n = unknown at position 189 <220>
<221> misc_feature <222> (451)..(451) <223> n = unknown at position 451 <220>
<221> misc_feature <222> (495)..(495) <223> n = unknown at position 495 <220>
<221> mist feature <222> (547)..(547) <223> n = unknown at position 547 <400>
231 ttttagaaagtttatcttcaatnaattttaattttttaatttatttatta 60 aaggatttaa ttttattttagaattgggttgactgtatgttattcttttcgactaagaagaatattattt 120 tttaattcttttaattattttagtttaaattttttaaatgatgaatttaataaaataatt 180 aaaataagntttattttaataattttttctttatgttttggtaagattatgattaatttg 240 attttttatattcctaatatattttttattagatttataataaaaattatagttataatt 300 atatcttattaggattatgagtagggttagaaattaggtattataaaattgggtataatt 360 attttaatagaaaaaaattagtattttttggaaatatatgatttttccagtaatttctgt 420 gtttatatttataaaccgtaattatggaatnatttaataaagatggtgacaaggatgatt 480 agaattataggatcnaaaactattttaaatattcttattatctaaaaattttataattca 540 aataatnta 549 <210> 232 <211> 548 < 212 > I7NA
<213> Ctenocephalides felis <220>
<221> misc_feature <222> (531)..(531) <223> n = unknown at position 531 <400> 232 tatgcccctc aacattcgaa gagaatctga ttcctacaaa tttcaaaggc atagaagatt 60 ttgtgcaagc aactgcccac ggtaaagctc aagaagtttt ccaaaggcta agtgaacaaa 120 FC-8-C2-PCT.ST25.txt atacaccacccgatgttgttataggggctgatacaatggtcactttagacggtatgcttt180 ttggtaaacccaattctgaagaggactcttataacacactatcaaggttgtcgggaagag240 tttgtaccgtgtacacaggagttgtaatcaaaacacagcgcaaaacggtcaagttttgtg300 acaataccaaagtccattttggaaaactcactccggcagtgataagagcatatattgcat360 caggagaacccatggacaaagctggaagctttggagtccaaggacgaggtgctagtctta420 ttgaaaaaatggtggtgattattttacagttgtgggttacctctacataaattatgtgtc480 gagttaaattcttatgtgaaaatattaatatattataatgtgatattttgnaaatatatt540 ggttattt <210> 233 <211> 549 <212> DNA
<213> Ctenocephalides felis <220>
<221> misc_feature <222> (547)..(547) <223> n = unknown at position 547 <400>

cttgtcatatattagtgaaaatgttattacaaataacgattgttttaagcactattggat60 tagtttgtgacaggtgccacgggaatgaaatcattacccagtttgatgaagtaacagagt120 cagattttgcatttccagaagattttcaatttggttgtgctactgctgcgtttcaagttg180 aaggagcatggaatgaaagtgctcacttttgcttattaggaaagggagaaaatatctggg240 atagactgatacattccaagccagatttagtaaccgatcttacaaacgctgatgtcgctt300 gtgattcttatcacaaatttaaagaagatgtggctgcactcaaaactattggtgtcaatt360 tttatcgtttttccatatcgtggtcacgagtgcttccactggtgacgcatccgtaattaa420 cgaggaaggaatccaatattattctgatttaatagacgaattactaagaaacggattgaa480 cccgtggtgactctattcactgggatgtcacagccttacagaattggaggtttataatcc540 gtcatancc 549 <210> 234 <211> 549 <212> DNA
<213> Ctenocephalides felis <400> 234 attcttcacc tgattttttt aaaatctaaa gattttcaaa aaaaaattac gattttttct 60 cgtgatgttt ggcggggaag gggggtggta attctgaata ttacccaggg gggatagaag 120 gtccagaaat ccttaaaaag ttgatcacgt tattaatgga caccccctaa ttgatgatac 180 aaagatgaga atttacatat ttctaatttc tattaatcac gtgaggtttt taggggggga 240 FC-8-C2-PCT.ST25.txt agggggtttatagtaagatatcacgtgtatttattttttacctgatttttttaaaaaact300 caaattattttaaaaaaaatgaagatttttacgattttttcgtaactaaaaatacacctg360 atgtttgctggggcgatgaggggggaattttgaatattaccaagggagagggggttccag420 aaatccttaaaaaatttatcatgtgaccaatggCgCCCCCttaggattaatatgcagtaa480 aagttttttattcttatttatttaaaaaaatgccatgttgtggtcccaataaaaacgtac540 gatctattt <210> 235 <211> 186 <212> DNA
<213> Ctenocephalides fells <400> 235 aatggacaaa caatttgtta ccttcccgtc aatttgggta tgtagtctta acaacgagtg 60 gtggtattat ggatcatgaa gaagctaggc gtaaacattt gggaggcaaa atattaggat 120 tcttctttta agctgtatta tgtcctgaat aaatcagatt tctacaacaa aaaaaaaaaa 180 aaaaaa 186 <210> 236 <211> 3 43 <212> DNA
<213> Ctenocephalides fells <220>
<221> misc_feature <222> (33) .(33) <223> n = unknown at position 33 <220>
<221> misc_feature <222> (39) .(39) <223> n = unknown at position 39 <220>
<221> misC_feature <222> (323)..(323) <223> n = unknown at position 323 <220>
<221> misc_feature <222> (325)..(325) <223> n = unknown at position 325 <220>
<22~> misc_feature <222> (330)..(330) <223> n = unknown at position 330 FC-8-C2-PCT.ST25.txt <220>
<221> misc_feature <222> (334)..(334) <223> n = unknown at position 334 <220>
<221> misc_feature <222> (340)..(340) <223> n = unknown at position 340 <400> 236 aagaagttgaaaagattgtcacaattatgtctnccccangacagttcaagattccagatt60 ggtttttgaatagacaaaaggatattgttgatggtagatacagtcaattgacctcatcca120 atttagactcaaaattgcgtgaagatttggaacgtatgaagaagatcaaggcacacagag180 gcatgcgtcactattggggtctgcgtgttcgtggtcaacacaccaagaccactggccgca240 gaggtcgtactgttggtgtgtctaagaagaaataagatacatatgtatttttttatttaa300 taaattcttattctacaccatanantaaanaaanaaaaanaaa 343 <210> 237 <211> 549 <212> DNA
<213> Ctenocephalides felis <220>
<221> misc_feature <222> (421)..(421) <223> n = unknown at position 421 <400>

aaacatgtcacactaagtcatcgtatgaaacgtgttccaagaactgtttttcccaaaaat 60 atgaccatggtcgtaaaagaagttactgaaatacttgaagatggtgccaagtttgaagat 120 ggttcaatagagaaattcgacattattttatattgcacgggatatctatatagttttccg 180 ttccttagtcctgagtgtggaattaccgtatctgaaaattatgtccagccattatataaa 240 catttagttaatatcgaacgtccaacaatggcactaataggcattcctttttacgtttgt 300 gcagctcaaatgtttgatttgcagattcggtttgtagttgagtatttgtgtggcaaattt 360 caattgccgtctaaagaagaaatgagagcggatactgaacgcgacatgcaaaaacgatgg 420 ncacgtggggatagcaaaaggaaagcccatattatgggcccagatcaggaggcctattac 480 aatgatttagcactactgtggatactcaattcccagtatgctaaatccaatgtgtagcga 540 agattcttg 549 <210> 238 <211> 549 <212> DNA

FC-8-C2-PCT.ST25.txt <213> felis Ctenocephalides <400>

ggaggtggtactggatctggtatgggtactctacttatttctaaaatcagagaggaatac60 ccagacagaattatgaatacttattcagtagtaccttcacctaaagtgtcagacactgta120 gtagaaccttataatgccactttgtctgttcatcaattagttgaaaacacagatgaaacc180 tattgtatcgataatgaagctctctacgatatctgcttcagaactttgaaactcaccaca240 cctacatatggagatcttaatcatttagtttctttaacaatgtctggagtcactacttgc300 cttagatttcctggacaattgaatgcagatttgaggaaattggctgtgaacatggtacct360 ttccctcgtttgcacttcttcatgcctggatttgcaccactcacatctaggggaagccaa420 caatacagagcactcaccgtccagaattaacacaacaaatgtttgatgccaagaacatga480 tggtgatgcgatccccgcatggtcgtacttgctgtggcactggttcagaggcgcatgcat540 gaaagaagt 549 <210> 239 <211> 549 <212> DNA
<213> Ctenocephalides felis <220>
<221> misc_feature <222> (537)..(537) <223> n = unknown at position 537 <400>

gtggcccaggacccatgcgaggtggtggccgtgacatgaatcgtggagcaccctattctc60 gtgatggtggtcgtggaggagattttggtggacgaggtgctgggcccatgcgcaatggta120 tgggcggcggcggcatgggtggtggttatggtgaccgcggcggttctatgatggacggcg180 gcatgggaatgcgtggtaatggcgttggagcaggtggaatgatggaccgcagaggtggcg240 gcatgggaggcggaatgggcggcggtggcggttacccggatggcggattttctggtgagg300 acagacgcgggttcgcgctgccgcagcttggcggcggcggtgcgggcggcgcttacagag360 acttagacttaggtcaaggtggttatgataacatgtacatggatgaccgaagaggtggag420 caccaccgatgatggatgataggcgaggcctgcaggtccaatgcgtcaccaatgcccatc480 ccccaatgtttgacaggaggcgctgtgtccaggagcatggtggtggacccgtgattntca540 caaaattct 549 <210> 240 <211> 549 <212> DNA
<213> Ctenocephalides felis <400> 240 FC-8-C2 -PCT.ST25.txt tctcattgtcgatctcattactccacgatactccgtacaggagacactttacatagaaat60 taagtgcgtggtgaaatcattcaatcgaccattaaatttaagttaagttcagttttttat120 caagttatttaagtgacagttaagtgccgtttttcgatccatgttctgtgcaaaaacgac180 aggatgacgttgaacgacgaaacagcaacggatgccatcgttaacggcgatggccttgac240 cgagacacgggagggctggaaaaggaccctaacacaaggaagaaaatggagttatcgctg300 catgaacgcgatagagttggggtgcaagattctgttcttttagaagactacacaaatgaa360 tcagttttcgtcgataatcttaagaagagatttggagaagaccttatatatacatacatc420 ggcaagtactggtatccatcaacccttacaaagaactaaatatatacagagaaaagatgt480 tttgaatatcaaagaagcgttttccagcctacctacgtattgcggtgagatatgcattag540 atcactgct 549 <210> 241 <211> 549 <212> DNA
<213> Ctenocephalides felis <220>
<221> misc_feature <222> (521)..(521) <223> n = unknown at position 521 <220>
<221> misc_feature <222> (547)..(547) <223> n = unknown at position 547 <400>

cttaaggccaaggctgagaaggagaaggcccaatacttcggtgaattgaatgaacttcgc60 tccggagttgaccaccttgctaacgagaaggccgcacaagaaaagattgccaagcaattg120 caacacagcctcaacgaagtacaaggcaaattggatgaaaccaaccgcaccctcaatgac180 ttcgactccgccaagaagaagttgtccattgaaaactccgacctcttgcgccaattggaa240 gaagccgaatcacaagtttcacaattatctaagatcaaggtgtcattgaccacccaattg300 gaagacaccaaacgcttggctgatgaagaaagccgcgaacgtgctacacttttgggcaaa360 ttccgtaacttggaacacgacctcgacaccgtccgcgaacaagttgaagaggaagctgaa420 ggcaaggccgtttgcaacgccaactcagcaaggctacgccgaagctaattgtggcgctcc480 aaatccaatctgaggagtcgccgtctgagactcgaaaagcnaagtaattgcagccgtctg540 ttaactnaa 549 <210> 242 <211> 426 <212> DNA

FC-8-C2-PCT.ST25.txt <213> Ctenocephalides fells <220>
<221> misc_feature <222> (3). (3) <223> n = unknown at position 3 <400>

aantggtgcagcgtttagtataccgacgacgtttgtcgtacaacacaaaatctaacagga 60 ggagggttgtccgaactccaggtggtaaactagtgtatcaatatttgaagaaacccaaga 120 agatccccaggtgtggacaatgcaaagacaaactcagaggcatccagcctgccaggccca 180 tggaaaggtccagaatgtgcagaagaaagaagacagtacgtagatcatatggtggagtac 240 tctgccacaagtgtgtaaaagaacgtattgtcagggcttttctaattgaagaacagaaaa 300 tagtcgtaaaagttcttaaagcacaacaagcaagtgttaaagcggctaagaaggataaat 360 agacctaattctatgttaaatataacataataaaaactttctcaaataaaaaaaaaaaaa 420 aaaaaa 426 <210> 243 <211> 485 <212> DNA
<213> Ctenocephalides fells <220>
<221> misc_feature <222> (436)..(436) <223> n = unknown at position 436 <400>

aatccaacgctttgcaatcgaatttatttagcatatcaggagattcttggcaaaaattaa 60 gaaatgctctgtcaccggcatttacatcgggaaaacttaaagctatgatggagattatta 120 ttagatgcggtaaaaatttgcaagaatttcttgaagaaaaatccaacagtaaagaagaac 180 tcgaattcaaagacttggctgcaagatacacaacagatgtaatttcttcagtagctttag 240 gcaatgaagtaaactcagtgaaaaatccaaatcacgaatttcgcgaaattggcagaaaaa 300 tatttaatttacccacaagatgggatgttgcatgtcattatgtttttcatgtcacctgaa 360 atcctgaataaatttaaattggcaaatataagtcgtgaagtttatgatttcatgatgaat 420 gtgtagacaaacagtncctttagagaagaaaataatatacagacctgttcatgcactttt 480 gatca 485 <210> 244 <211> 549 <212> DNA
<213> Ctenocephalides fells <220>

FC-8-C2-PCT.ST25.txt <221> misc_feature <222> (531)..(531) <223> n = unknown at position 531 <400>

atattcttcacaattattgcagcgattcatttcacaggacacagggtgccgtatgatttt60 gtaaataaaattgtaccattattaccagggtctaccacaggctggcagctaacaggatgt120 gccataatggcactttgtctatggttaactataattttcgttttaagatacacatttaaa180 ttgttactgatgtacaaaggatggatgtatgaggccagagggaaagggacttctgtttcg240 ttaccgacaaagatttggcttgtgctagtcaaaatcatgtccggttggaacaaaccagga300 ttgtacagttatcaaggatcactgccaagactgcctttaccatcggttgaagacacaacc360 aaaagatatcttagaagtgtccgtcccttattggatgatgccagttacaagcgcatggaa420 ggtctgactgaagaattcaaaaaaggcattgcagtgaaactgcaaagatatctcttctga480 aatctgtggcgagcactatgtctcagatggtgggaggattgttattgaganggagaggac540 cttatggtg <210> 245 <211> 549 <212> DNA
<213> Ctenocephalides felis <220>
<221> misc_feature <222> (498)..(498) <223> n = unknown at position 498 <220>
<221> misc_feature <222> (533)..(533) <223> n = unknown at position 533 <220>
<221> misc_feature <222> (543)..(543) <223> n = unknown at position 543 <220>
<221> misc_feature <222> (546)..(546) <223> n = unknown at position 546 <400> 245 attttatcga tttgcttgtt ttcgtgtgca tttttgacgt ttcatatttt tcgaaaacgc 60 ccataagtat taaaacttaa aatggccagt tacagtgaag atcaattggc agagttccaa 120 gaagcctttc aactatttga ttctcggggt gatggtaaaa tagatgttag cttggttgga 180 FC-8-C2-PCT.ST25.txt gatgctttgagagctcttggtcaaaatcctactgaaactgaagtcaaaaagttcacccat240 caacttaaacatgatgaccgcatttcttttgaagtatttctacccatatatcaggctatc300 tcaaaagggcgctcagcagatacggctgaagattttattgaaggtttgaggcactttgat360 aaagatgggaacggttttataagttgtgccgaattaagacatttgctttgtactttgggg420 gaaaaacttactgtgaggaggtcgagcaattgcttcaaaaccaagaagatctcaagcaac480 atcaattatgaagattgnaagaatggcatgtccggtgaatcataaattgaagntttaata540 acntcntca 549 <210>

<211>

<212>
DNA

<213> ocephalidesfelis Cten <400>

gctgatgatgctgatgtagatatggttgtaagagcagcagtatttgcctgcgttggaact60 gctggtcagaggtgcacaacaacccgacgattaatcgtacacgataaagtttacgatgaa120 gtgatctcacgattgaagaaagctttcgctcaagttcttacaagaattggtgatccattg180 gacaaagatactttatatggacctctacattctgaaaattctgttaatgaatacaagaga240 acgattgaagaagctaagaaattgggcggtaaaattgaatttggaggcaaggtggtagac300 aggcccggattcttcgtcgaacccacaattgtttctggattggcccatgatgcccctgta360 gttcgcagcgagtgctttgccccaattgtatacgccttgagagcctcttctgtccaggag420 gccttcaaatggaataatgaagttgaccagggattatcatccagtctcttcacccaaagt480 attagcacgttttcgattggataggccatgggtcagactgcggattggaacgtcaacatt540 ccacatcag 549 <210>

<211>

<212>
DNA

<213> felis Ctenocephalides <400>

ccaaaatgaagttgaatgtgtcttacccggcaacgggatgccagaaattgttcgaaattg 60 tggacgagcacaagcttcgtgtcttctatgaaaaacgtatgggagcagaagtcgaggccg 120 acatcctcggcgatgaatggaaaggttatgtactccgaattgcaggaggaaatgacaaac 180 aagggttccccatgaaacagggtgtcttaactaatgttcgtgtacgtttgctgttgtcaa 240 aggggcactcctgctaccgcccccgtcgtactggagaacgtaaacgtaaatctgtacgtg 300 gttgcattgttgacagcaacttgtctgttttagctcttgtcattgtacgcaaaggagtca 360 atgaaattcccggattgaccgacggcaatgtaccaagaaggcttggacccaagagggctt 420 caaaatccgtaagctgttcaacttgacaaggaagatgatgtacccgtatgtcgtcagacg 480 FC-8-C2-PCT.ST25.txt tccacttcca gtcaaagaag gtaagaaccc gtacaaaggc ccaagatcca aagctgtcac 540 tccacatct 549 <210> 248 <211> 549 <212> DNA
<213> Ctenocephalides felis <400>

attaaaaaaaaatctcaaatcgatcataaaagttttaatattaatttcagtaattaatac60 aacttctggatcagatgctaacaaatacgaagccacctgggagagtttggatagcagacc120 atctcccagttggtatgatgatgccaaagttggaatatttttacattggggagtttatgc180 agtaccaagttttggcacagaatggttttggcaaaattggcaaggatctaatgtgtcctc240 ctacgtggattttatgaatcagaactatcgaccgggatttacatatcaagattttggtac300 agaatttactacggaactgtttgatccaaaccactgggctgagctattccaagcttctgg360 ggctaaatatgttgtgttaacaagcaaacatcacgaaggatacaccctgtggcatcaaag420 tactctttcagttggaactcccaggacgttggtgctcataaggatttaataggtttacta480 ccaacgcaattcgcagcaaaaccaactacgtttcgtctctatcactcttgtcgatggtca540 cagacttat 549 <210> 249 <211> 550 <212> DNA
<213> Ctenocephalides felis <220>
<221> misc_feature <222> (454)..(454) <223> n = unknown at position 454 <220>
<221> misc_feature <222> (519)..(519) <223> n = unknown at position 519 <220>
<221> misc_feature <222> (529)..(529) <223> n = unknown at position 529 <220>
<221> misc_feature <222> (535)..(535) <223> n = unknown at position 535 <400> 249 FC-8-C2-PCT.ST25.txt gttatcaacaggattttggagatcgtgcaaggtgtttccaacctagtctaacttcttcat 60 cttcaatatgtgatgaacgattcacttggaaccctgataatgagcaaagaatgattttac 120 aagaagctctgtccaaagcacacactcatttaggcactggagcatcaataggttccggtt 180 ccatttctggtagctccattggttcttcttctacgtcgtcttcctcatctagttcttcct 240 caagttcttcttcatggtctggcagcggaatgggagttagttcaactcaaggacaaaagg 300 aaattgttcaaatcagcccacaaaaagtagggcttaaattaagaattaatgaagttcata 360 aactaaatgtttattatgcacaagctgagattatccagtagattatattatttgatggac 420 ttgctaagtctatggaagatgataagagagatgncagcattggagattactctcagaacc 480 tgagaaatatactcaaattccggtgggtttggactttgnggataagtgngatgcntatgt 540 gcaacatgcc 550 <210> 250 <211> 472 <212> DNA
<213> Ctenocephalides fells <220>
<221> misc_feature <222> (440)..(440) <223> n = unknown at position 440 <400> 250 gcaaataattctgtgttgatatatcttgtcatattaaaaacaagtgaacttgtgaaaatt60 tgcaattaatttgggtgacaataatgagacttattatatttatatcgtgcttggcatcaa120 gtacggtttaaatgtacctatagtcaaaggcataataaaaacgctagtggaatttgcacc180 aagtgttattgtataaaactgcaatatgatcaatataaaaagtatatgtccttatttttt240 tgtttatattttactttcatcttatataaacactattttatgtatcccggtaccaatgag300 gcgctgtgcaaagttatacaactcaataagtcctcaagcctcggcagagtacgatgagaa360 tggcgatatatcaaaagatctggtcgaaaatctcttacacaggtgtagaacaatgcacaa420 gtaattttgtactcttgtgncggaaaatgaaacggtctctcatgtatgggca 472 <210> 251 <211> 339 <212> DNA
<213> Ctenocephalides fells <220>
<221> misc_feature <222> (13) .(13) <223> n = unknown at position 13 <220>
<221> misc feature FC-8-C2-PCT. ST25. tact <222> (54)..(54) <223> n = unknown at position 54 <220>
<221> misc_feature <222> (108)..(108) <223> n = unknown at position 108 <220>
<221> misc_feature <222> (139)..(139) <223> n = unknown at position 139 <220>
<221> misc_feature <222> (274)..(274) <223> n = unknown at position 274 <220>
<221> misc_feature <222> (305)..(305) <223> n = unknown at position 305 <220>
<221> misc_feature <222> (311)..(311) <223> n = unknown at position 311 <400>

ggtcctaatttanttaccccattccaaaaattttaaatttttgccaaaaatgcntttcta60 ccatggttaaagctcaaatatccggacttttttattgccttattattngatgaataattt120 cgattatttgatttcgatncaaaatctaaatgaaccagtttaaatccaaatcaaataatt180 gaaattatttgtaacaaaaaaaaaatttataatcccaaaaatataaaaaatatttatttc240 tactcattgaacacaaaattcacatagaaaatgngcctgtttgctatacaatatagatta300 aaatntacgtncgtattttaataatttttgcatatttat 339 <210> 252 <211> 549 <212> DNA
<213> Ctenocephalides felis <400>

ccggcagaccgtttttgtttgctgcattactcttgttggcgaaattagtgcacgatggct 60 caaccactagctacatctgatatcaagtataccaagctattcatcaacaatgaatttgtc 120 gattctgtaagcggaaaaaaatttcctactattaatccaaccaatgaaaagaaaattata 180 gatgtatcggaaggtgataaggccgatgtagatctggcagtcgctgctgccaaaaaagcg 240 tttgcgctaaactcagcatggcgccagatggacgcatctgcacgcggaaagctacttaac 300 FC-8-C2-PCT.ST25.txt aagtttgctgatttgattgaaagagatcttcaatatttagcaaatttagaaaccttagac360 aatggaaaaccattttctaatagtatcatagatatacagtgcgctatcaataccatcaga420 tattatgcggctggtgcgacaaaattcatggtcatacaattcagcagattatggattgcc480 agcttcacattcgacaccagtggtgtagtaggtcagatttaccttggacttccaatgcta540 tggtgcctg 549 <210> 253 <211> 549 <212> DNA
<213> Ctenocephalides felis <400>

aacagctgtttgtgccgtacggtggtccccgcgtgtgtagcattgtagcagtatcaaagc60 gaagctcgatcgatccggtcgagacgtgaagcccgcgcgatacccccgcttcagagacaa120 tttacagactccggagttaatatcacgaaaccactttgtgttcgacttctcagtacgaca180 gtgtcggtcatttcaaacgcgcgttgtgtgtgtccatctatcatttacaacggggttcct240 gatttttcgaaggttcagtaataattttcgtatttcgttatggcgatgagatgtgtagga300 ttgctcgtcaagagcaccatggtccagtccggacagcgcaggctgatatcccaatcagct360 gttgctatgaacaggatgcttcaggattcaccaaacgctcacctagataaatcaatcttg420 caaaggtacactcgtcttctcaagacgatcggtcacgcaggtcacttacgtgtggatcga480 tggacggtgagtacttgccgctaaggacgtgtcttgaattaacctccaaaccagaaactg540 caaatggac 549 <210>

<211>

<212>
DNA

<213> felis Ctenocephalides <400>

gttgattatagtccttgacctttaactggagctttaggaactttaagtttaacattaggt60 ataattatatgatttcatcaatttaattatacattaatattattaggaataataattacc120 ttattaactatatttcaatgatgacgagatattagtcgagaaggatcattccaaggtctt180 catagtttaatagtttctaaaggattacgaatcggaataattttatttattatttcagaa240 attttattctttttttctttcttctgagcatattttcatagaagattatcaccttctatt300 gaattaggaataatttgccacctttaaatattaaaatatttaatccttttcaaatccctc360 tattaaatacagcaattttattatcttcaggaatttcaattcatgagctcatcattcatt420 actagaaaaaaatcattcagaaacaataaaaagttatttattcaattattttaggaatta480 tttttctatacttcaatggatgaattttagaagctcttttcaattgagatcaattttgga540 FC-8-C2-PCT.ST25.txt caatttttc 549 <210> 255 <211> 465 <212> DNA
<213> Ctenocephalides fells <400>

gtttgtaacggcttccaaagatatgactgctaaactttttgacactgattcgcttttatg 60 tttgaaatgttacaaaacagaaaggcatgtaaatagtgccgccatcagtcctattaaaga 120 acatgttgcattgggaggtggtcaagatgctatggaagtaacaaccacatcctcaagaca 180 gggtatgtttgaaactagattcttccacttggtctatgaagaagaattcggtcgtgtgaa 240 gggacatttcggtccagtaaacagcttgaagttccatcctgatggaaagagttttgctac 300 aggaggagaagatggttttgttcgagttcaaaattttgattatacatatttagattatgc 360 ttttgaataaacttgagaattttcatatttgtaatttgtccaatttatacaatgttcaag 420 atgatgaaaataaagtataattatactgtaaaaaaaaaaaaaaaa 465 <210> 256 <211> 549 <212> DNA
<213> Ctenocephalides fells <220>
<221> misc_feature <222> (3). (4) <223> n = unknown at positions 3 and 4 <400>

ttnnaccgtagctctaaagtgacgttacaatgtcgggaggcttggatgttcttgccotac 60 gggaagatgatgttaccaaaatgttagccgcatgcacccatcttggcagcgaaaatgtga 120 acttccagatggagcaatatgtgtacaaacgccgtgctgatggagtacatgtaatcaact 180 tacgtcgcacatgggagaagcttttgttggCtgCtCgtgCtattgttgctattgagcatc 240 cagctgatgtcttcgttatttcctccagacctcaaggacaacgtgcagtattgaagttcg 300 ccaactatactggtgctactccaattgctggacgtttcactcctggtgccttcactaatc 360 agattcaagctgcattccgtgaaccacgtttgctgattgttactgatcctcactccgatc 420 accaacccatcactgaggcttcatatgtgaacattcctgtattgcattcaccaacactga 480 ttctccattgaaatttgtgatattgaattcatgcaacacaagtccaaccatccgtggttt 540 gatgtgtgg 549 <210> 257 <211> 0 <212> DNA
<213> Ctenocephalides fells FC-8-C2-PCT.ST25.txt <400> 257 <210> 258 <211> 549 <212> DNA
<213> Ctenocephalides felis <400>

gtggtgttagccgcgactgtttcaactattttagcggcaagttgttcaaatccagaagtt60 acagccacatctttcactacgcaagatgctacaattgttacaaatatagcatttattgct120 gaatttacattaaaatgcagtccaagtgttcctagtggcatacctttgtatgctgaaatt180 gatggtaaagtaatgccgactgctagaattggagataataaataccaagttagctggact240 gaagaaattaacaaagctcgcagtggtgatcataaagtgagattatatgatgaagaaggt300 tatactgcagttcgtaaagctatgcgatctggtgaagatgccagtgctgtcaggtcattg360 gtgactatcgttatttcacatcctggtgcatttactggtccatgggtcaattctgaattg420 gtgctcattagctgctgtggtgtagcttacttagcattacttcaaatacaaattattatt480 atagatgatttaattatagtaatgtaaaatttattattaaatacaatctgattttataaa540 aaaaaaaaa 549 <210>

<211>

<212>
DNA

<213> felis Ctenocephalides <400>

agatggttgtggtgatctgaatatttagtataaatttgtgtttttagcgttcatttatca 60 gttaagtgatcaaaaatgtctttcaatcaagatattaaaacagaaaacggtggtgaattc 120 gcccaagatgttgaaatgaaaactcacaatacagacggcagtggtgacgcatcaacaaca 180 caaaatggacacgcgggaaagcctcgtttcgacgatagaaaattattcatcggtggactg 240 agtgccgaaactaccgaacaacaattacgcgatcatttcgttcaatacggtgccatcgac 300 tccgtggatgtgaaaagcgacccaaataccggcagatcaaaaggattcgcatttttagtg,360 ttcgcgtccgtggactccattgaaaaagcgcttgcggtcagtgaacatgtcattaattct 420 aagaaaatagacgttaagaaagcaaaagcgaaacacggaaagttttcgtcggcggttaaa 480 tacaacaataccgcgatgatattaaaactcatttcggcaatttggaaattcgcgagtcga 540 ctcccttgt 549 <210> 260 <211> 549 <212> DNA
<213> Ctenocephalides felis FC-8-C2-PCT.ST25.txt <220>
<221> misc_feature <222> (473)..(473) <223> n = unknown at position 473 <220>
<221> misc_feature <222> (521)..(521) <223> n = unknown at position 521 <220>
<221> misc_feature <222> (526)..(526) <223> n = unknown at position 526 <400>

gaaacttggcgcttattggagcacttcttcttgtacttgctgaatctcgagtggaaggaa60 gaagtctttttgctggaattcctactatgggtgattcaaggccaaaaaattatttacaac120 tggctggcagaattttactagctttcatgtttattacattaattcgttttgaattaagtt180 ttttacaggttatacaagatttaattggatctgttttaatggtattggtaaccattggtt240 ataaaacaaaattatcagctctgattttagttctacttctttcatttttaaatttatacc300 acaatgcttggtggacagtgcctacctataagccattaagagattttctcaaatatgatt360 tctttcaaacattgtctgtgattggaggtcttcttatgattgtttccttgggcccaggtg420 gagtttcgatggatgaacataaaaaagagtggtagaaaaatcaggaatattgngccgtgt480 gctagtataacaattatatatatatatatatatatatatcntatcntatatatatatata540 tatatatat 54g <210> 261 <211> 549 <212> DNA
<213> Ctenocephalides felis <400> 261 cgtaaaatca agaaacttct aagaaaatta atgtcactgt taaaacacca aaagagaaac 60 aaacagtgga agttgaggaa gatgccgaaa ttaaagactt caaaactttg gttgctcaga 120 aattcaacgc agaacctgag caattatgtc ttatttttgc gggaaagata atgaaggata 180 ctgatacttt gaaaggtcac aatattaagg atggtcttac tgtacattta gttatcaaaa 240 ctcctccaag gcctgagcca gaaagggcag ctcagagacc tccagctgat atcagtgcaa 300 caccttttgg attaaattca ctaggaggtt tagttggatt agagtcattg ggtttaggtc 360 aaggttcatt catggattta caagcacgga tgcaaaatga actcatgact aaccccgata 420 tgttaagaaa tgttttagat aatccacttg ttcagcagtt gatgaatgat cctgaaaaca 480 tgagacctta attacatcta atcctcaaat gcaagattaa tgcacgcaat ccagagattc 540 FC-8-C2-PCT.ST25.txt tccatgctc 549 <210>

<211>

<212>
DNA

<213> felis , Ctenocephalides <400>

gctgtacactcacgctctcgtgtacgtaaaaatactgaagccagaaggaaaggtcgccac60 agcggttttggtaaaagaaaaggtacagctaatgcccgtacaccacaaaaggatctatgg120 gtacaacgtatgagagtcctgaggcgtttgttgaaaaaataccgtgaagcaaaaaagatt180 gatcgccatttgtatcatgcactttacatgaaagcaaagggtaatgtattcaagaacaag240 agagttcttatggaatacatccataagaaaaaggctgaaaaggcacgtactaaaatgttg300 aatgaccaagctgaagctaggagaacaaaagtacgtgaagcacgtaaacgacgtgaagaa360 agaattgctactaaaaagcaagagctcttacaaacttacgctaaggaagatgaagcagca420 gccccaaaaaataaatgtcccttccttgtaaataaaataaatttatataaatttaaaaaa480 aaaaaaaaaaaaaaaaaaaaas 502 <210> 263 <211> 549 <212> DNA
<213> Ctenocephalides felis <220>
<221> misc_feature <222> (32) .(32) <223> n = unknown at position 32 <220>
<221> misc_feature <222> (512)..(512) <223> n = unknown at position 512 <400>

ggttactatgcctttctcggttcgagaaggtnttaacatatttttggacggttatgtgcc60 aactgaaaacatgagattcaggactgaatcgcttgtttttaaagtagccgaatcggcaac120 cgaggaagaggtgaaacgtatgaaccattacgtttacccggcaatgtcaaagacaatggg180 cgatttccatctggaaatcaaacagggccagttttcggattccgaaattttagtattatt240 gggcgaaaacggtaccggtaaaacgacattcatcagaatgttggccggaaacttggaacc300 tgatgcaggttctgcgaagttgccacaattacacataagttacaaaccgcagaagatttc360 accgaaatctcaagggcacgtgaggcatttgttgcacgagaaaattagggacgcatatat420 acatccacagttcataacagacgtaatgaaacccatgaaaatcgaagaaatcatggccaa480 FC-8-C2-PCT.ST25.txt gaagtccaaa acctctcggc ggcgaactgc ancgcgtcgc ctcgtcttgc tcggaaaccg 540 gccgcgtct <210> 264 <211> 534 <212> DNA
<213> Ctenocephalides fells <400>

attcgtgaaaaatattaatttaaaaatgcagaaacaaaagagggaacctattcatgctgt60 ccaagtttttggcagaaaaaagacagccaccgctgttgcctattgtaagcgtggacgtgg120 aaatttgcgagtaaatggacgtcctttggaactcgtggagccaaaagttctccaatacaa180 gttgcaggagcctattctacttttaggcaaggaaaaatttgccggtgttgatatccgcgt240 tcgagtaaatggaggaggtcacgtcgcacaaatttacgctatcaggcaagcaatttcaaa300 ggctttagtttcctattaccagaaatatgtcgatgaggcgtctaagaaggaaatcaaaga360 catgctgattcaatacgacaggaccctgttcgtagcagatccccgtcgttgcgaaccaaa420 gaaatttggaggtcaggtgcccgtcacgttaccaaaagtcatccgtaatttatattatct480 ttttttaaattctattataataaattggttgcagaaaaaaaaaaaaaaaaaaaa 534 <210> 265 <211> 549 <212> DNA
<213> Ctenocephalides fells <220>
<221> misc_feature <222> (43) .(543) <223> n = unknown at position 543 <400> 265 gtgaaaaggccattctcatcgatggtcgtggccatctgttaggccgactggctgctatta 60 ttgcaaaagttttgttagaaggaacaaaagttgttgtagtaagatgtgaacaactgaata 120 tttcaggaaacttcttcaggaacaagttgaagttcatgtccttcttgcgtaagagatgta 180 acatcaaccctgcacgaggaccttaccatttcagagcaccctccagaattttgtggaaga 240 ctgttcgtggtatggtgccacacaagaccgaaaggggaaagcaagctcttcgcagactta 300 aggcatatgaaggttgcccagcaccattcgacagaaggaaacgtctagttgttccaaaag 360 caatgagagtcatgtgtcttaaacctgccgcaagtactgcatgttggtcgcttatcaaat 420 gaggttggatggaaatatcaagatgttgtcgtactcttgaaaacaagaggagagttaaga 480 ctattcttaacatcaaaaagagaaataatcttaagaactcctaagaaggaggtgagcagt 540 cgncaaagg 549 FC-8-C2-PCT.ST25.txt <210> 266 <211> 549 <212> DNA
<213> Ctenocephalides fells <400>

gaaaaacaagcagtacttcaagcgctaccaagtaaaattcaaaaggcgtcgtgatggtaa 60 gaccgattactatgctcgtaagcgtcttacagtacaggacaaaaacaaatataacactcc 120 taagtatcgactgatcgtgcgcctttcgaacaaagatatcacctgtcaagttgcttactc 180 cagaattgaaggagacaagattgtgtgcgctgcttattcacatgaattaccaaagtatgg 240 tatcaaagtaggtttaactaactacgctgcagcatattgcacaggcttgcttttggctcg 300 aaggctcttacaaaagttcggcttggataatttatatggtggctgtactgaagtaacagg 360 cgaagagtacaatgttgaagctctggatgatggaccagctgctttccgttgctacttaga 420 tgttggtttagcacgtacaaccactggtgctcgtgtgttcggagccatgaagggtgctgt.480 gtggtggcttaaatattccacactccactaagcgtttccaggtatgatgctgaaagcaaa 540 agctcatgc 549 <210>

<211>

<212>
DNA

<213> fells Ctenocephalides <400>

ctgttaaaacatcaattacaatatgtcagacgtagaagttgatgtaccttcttcggcccc 60 agttcttgggggagccatggacgtaaacactgctttacaagaagtgttgaagactgcact 120 tattcatgatggacttgtacatggtttacacgaagccaccaaatctctggacaaacgaca 180 agcagttctttgtgttttggctgaaagttgcgatgaaccaatgtacaaaaaattagtaca 240 agctctgtgcaatgagcatcagattccccttgttaaagttgatagcaacaaaaaactagg 300 agaatgggcaggtctttgcaaaattgacaaagatggtaaagccagaaagattgttggatg 360 ctcatgcgttgtcatcagagatttcggggaagagactccagctttggatgttttgaagga 420 ctatttgaaacaatcgagttaaactatcttgctatttttaattatgaaataaaaatacaa 480 aaacctaaaaaaaaaaaaaaaaaaaaaaaaaaaa 514 <210> 268 <211> 549 <212> DNA
<213> Ctenocephalides fells <400> 268 aattcggcac cagggtcggc tttagtcgta acaaattgaa tatcaaaaaa taatgataaa 60 tttatcattc agtcttattc taaaatcgta gacgtgatgt tgttttggtt agttttaatt 120 tttgtgttgt gtttttatgc ctattttagg ctaaatcgag tgcgacgatt tgctgataag 180 FC-8-C2-PCT.ST25.txt ataccaggagaacaaccatggcctatagcaggaaacgccttagatattgggacggatcat240 acgaaattattacaaaaacttatgcaatatttggataagtataaattttctcgatttggt300 atttggcttgggatggactattttgtgtttttagtagaacctgatgatgttgagattatt360 ctaagcaacaccacaaatataaccaaatcaaaagaatatgattttctgcacaaatggctt420 ggagctggttattaacatccacaggcgataaatggcgacatagcccgaaaaatcattacc480 ccacattccactttaaaattttagaggaatatgcagaggtgatgaccgtgagtgtacttg540 gttaaaaat 549 <210> 269 <211> 477 <212> DNA
<213> Ctenocephalides fells <400>

cttcttcctctgattggtaacaaaacaatttcttattgggacagttttgcaatcacaaaa60 ttagtgtttcagaaaaatgagagctaagtggcgtaaaaagaggatgcgcaggcttaaacg120 taaacgcaggaagatgcgtgctcgttccaagtaaataaacgattgcaatcatttaacact180 tgtgtatatttttgcagtttattgtgaagctcattatcataatggccaaaatgaaatatt240 ttcgatattcaatcgacattcattatatttaagtaacaatagtatatgtatagcctgttt300 ctaaattattatgcaagcataaactaaaaattatttattaaacaaatgttatttaatata360 ttattgaattattatatatattgaattgaattgttataaacccgtattaatgaattatta420 taaacccgtattatacagaaatatatatttattaattgcaaaaaaaaaaaaaaaaaa 477 <210> 270 <211> 549 <212> DNA
<213> Ctenocephalides fells <220>
<221> misc_feature <222> (456)..(456) <223> n = unknown at position 456 <220>
<221> misc_feature <222> (495)..(495) <223> n = unknown at position 495 <400> 270 agagtattct gtatctatta ccatagtgtt ttgtttgttt aaatagtttt taaagataac 60 aaaatgaact taattatact tttcggcctg tgttttgtta cgttttgtac gggacatgag 120 gaaagccctt acacattcca atatacgaaa gagaactttc aagagggagt tggaaaaaat 180 FC-8-C2-PCT.ST25.txt aaccatttcatcatgttttatgcaccctggtgcggttactgccaaaaattggcgcccaca 240 tggaaccagttagccgaaatgttaaattcagatgagcattcaagagtcaccattgccaaa 300 gttgattgtacgactgacaatgaagtttgttctcaacatgatgttacaggctaccctact 360 ctgttatttttcaaattggacaccgaagaacctgtgaagttcaaaggaactcgagatttg 420 ccaactctcacagcttttattaatgaacaaattggngcaatgaacctttggatgttgaat 480 cgattccacatattntgatgctgcaatggctagttgaaccgcagaagataggttggagat 540 atgtcagag 549 <210> 271 <211> 549 <212> DNA
<213> Ctenocephalides felis <220>
<221> misc_feature <222> (443)..(443) <223> n = unknown at position 443 <220>
<221> misc_feature <222> (543)..(543) <223> n = unknown at position 543 <400> 271 gttagcccaaggtttaatagatcgtggccattttgataaagagaatataaaaattagaca 60 agctgatattgaaaaacaatttgacaatttgaaaaacttgagtttacaaagggaaaaacg 120 gcttgctgagagcaagaaattcttccactttattcaaatggtagaagaggttcaggagtg 180 gataaatgatcagatatcaattgcagattctgaagattatggaactgatgttgaacatgt 240 ggaattgctcataaagacttttgatacttttgtagcaggattaatagctagtgaaagcag 300 aattataaattgtacagacacaggaaagaaattgattaaagaaggaaatccatgcgctga 360 tactattcgagataaaattgatgaaacaaaacaattatgggatgacttaaaagaattagc 420 tcatgttcggcaagaggcattgntggtgctaaacttgtcatgtgtttgccgcaatgctga 480 tgaactatttcctgatagctgaaaagaatcgacataccactgccatacttcaagattaga 540 agnatccat 549 <210> 272 <211> 515 <212> DNA
<213> Ctenocephalides felis <220>
<221> misc-feature <222> (28)..(28) <223> n = unknown at position 28 FC-8-C2-PCT.ST25.txt <220>
<221> misc_feature <222> (145)..(145) <223> n = unknown at position 145 <220>
<221> misc_feature <222> (178)..(178) <223> n = unknown at position 178 <400>

ttgcccttagtctaaaatggggcgtgtnatcagagctcaaagaaaaggtgcgggctccgt60 cttcgtggcccatacaaagaaaaggaaaggggcacctaaacttcgttcactcgatttcgc120 tgagagacacggttatattaaaggngttgtcaaggatattatccatgaccctggtcgngg180 agctcccttagctatggttcacttccgtgatccatacaagttcaagaccagaaaagaatt240 gttcattgcacctgagggtatgtacacaggacagtttgtgtactgcggtaaaaaagctaa300 tctacaaataggaaatgtaatgccagttggtgctatgccagaaggaaccattgtatgcaa360 cttagaggagaaaactggcgaccgaggtcggctacccgtgcttctggaaactacgctctg420 ttattgctacaacccggcactaaaaagactcgtgtcaagcttcattggagctaaaaaggt480 atgcctttacatcgtgctatggtggattgggctgg 515 <210> 273 <211> 549 <212> DNA
<213> Ctenocephalides felis <220>
<221> misc_feature <222> (488)..(488) <223> n = unknown at position 488 <400> 273 aagcattctcatactttgcaacagctcaacaatggaaaaaaaattcctccaagcaattgg60 aaatgtgagaaatgtgatctcacctcgaatttatggcttaatttatcagatggatccatt120 ttatgtggcagaaaattttttgatggcacaggaggaaatgatcatgctgttgaacactac180 aaagcctatggttatcctctggctgttaaattgggtacaataactaaagaaggtaaaggt240 gatgttttcagctacgatgaagatgatatggtagaggatgttaacttaaagcagcatcta300 gcacattttggaatcaatataactgatttagaaaagactgaaaaatctatggtagaattg360 gagcttgatttaaaccagcgagtaggggagtggggtgctcttactgaaagctcaaatgcc420 ttgaaacctgttatggcctggttatactggctgagaaatttgggaaattcatgttatttg480 aacagtgnatgcagtttgtcactatccagacttattcttagattgcatgatcacctagat540 FC-8-C2-PCT.ST25.txt atcacacat 549 <210> 274 <211> 406 <212> DNA
<213> Ctenocephalides felis <220>
<221> misC_feature <222> (55) .(55) <223> n = unknown at position 55 <220>
<221> misc_feature <222> (349)..(350) <223> n = unknown at positions 349 and 350 <220>
<221> misc_feature <222> (352)..(352) <223> n = unknown at position 352 <220>
<221> misc_feature <222> (400)..(402) <223> n = unknown at positions 400, 401 and 402 <400>

cgttagctaaagatttattgcatccgacgattgcggaggagaagagacgtcacangctaa 60 agcgtcttgtgcagcatccaaattcttattttatggatgtcaaatgtccgggttgttata 120 aaattacaacagtttttagccatgcacaaagtgtagttgtatgtgctggatgttcaacaa 180 tattatgccaaccaacaggaggtcgtgctcgtctgactgaaggatgttcgttcaggagga 240 aacaacattaatttaagttatttcctactttgtttataagatgtaaaagttaaattgcct 300 tgtagatttttttaaaactctaataaaggcaaatttgaaaaaaaaaaanngnaaaaaaaa 360 aaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaannnaaaa 406 <210> 275 <211> 399 <212> DNA
<213> Ctenocephalides felis <220>
<221> misc_feature <222> (353)..(353) <223> n = unknown at position 353 <220>
<221> misc_feature <222> ('365) . . (365) FC-8-C2-PCT.ST25.txt <223> n = unknown at position 365 <400> 275 attacgcaga accttctgca aaaaatgcaa ggttcacaag caacacaaag taacacaata 60 taaaaaatca aaagaacgac atgcagccca gggacgtcgt cgttacgaca gaaaacaaca 120 aggttttggt ggtcagacca aacctatctt cagaaaaaag gctaaaacta ctaagaaaat 180 tgtattgaga atggaatgct ccgactgtaa attcaggaaa caaatcccac ttaagcgttg 240 caagcacttc gagcttggtg gtgacaagaa gaggaaggga cagatgattc agttctaaat 300 gtgtattttt tttacatacg actgtattaa gtttaataaa gcagtttaat gtnaaaaaaa 360 aaaantaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaa 399 <210> 276 <211> 549 <212> DNA
<213> Ctenocephalides fells <220>
<221> misc_feature <222> (417)..(417) <223> n = unknown at position 417 <220>
<221> misc_feature <222> (516)..(516) <223> n = unknown at position 516 <400> 276 gcttcagctcatttagtcggaggagcaaagaaggtcatcatttcagctcctagtgcagat 60 gcaccaatgtttgtttgtggtgttaacttagaagcttatgacccgtccatgaaagttgta 120 tctaatgcttcttgcactaccaattgtttagctcctttggctaaagttattcatgataac 180 tttgaaatcgttgaaggtcttatgactacagtccatgctaccacagccacccaaaagact 240 gtggatgggcctagcggtaagttatggcgagatgggcgtggagctgcacaaaatattata 300 cctgccgcaactggcgctgctaaggctgtaggcaaagtcattccggctttgaatggtaag 360 cttactggtatggcgtttagagtccaatagcaaacgtttctgtggtgatcttactgnaag 420 attgggtaaaaatgcacttatgaagatatcaagaacaaagtaaggagcatctgaaggctc 480 ttgcatggaatttggattatacagaagatgatgtgngtctgtgattcattggtgatccct 540 ctcgatctt ' 549 <210> 277 <211> 549 <212> DNA
<213> Ctenocephalides fells FC-8-C2-PCT.ST25.txt <400>

agttgccgccctcgtagtcgataatggatctggtatgtgcaaagccggtttcgctgggga 60 tgatgctccacgagctgtattcccatcaattgttgggcgcccccgtcatcaaggtgtgat 120 ggtcggcatgggacaaaaagacagttatgtaggtgatgaagcacaaagcaaaagaggtat 180 cctcacattgaaatacccaattgaacacggtattgtcacaaactgggatgatatggaaaa 240 aatctggcatcacaccttctacaatgaactccgagttgccccagaagaacaccctgttct 300 cctcacagaagccccattgaatcctaaagcaaacagggaaaagatgacccaaattatgtt 360 tgaaaccttcaacactcctgcatgtacgttgcatccaggctgtctgtcattgtcgcttct 420 ggtcgtaccactggtattgcttggatttggtgatggtgttctcacactgaccaatctatg 480 aaggatCgCCCtCCtaCCttcttgcgtttggacttgctgccgtgacttactgatattaat 540 gaaatctaa <210> 278 <211> 549 <212> DNA
<213> Ctenocephalides felis <400>

aatgaggtttgtacgataactattgtggcttttagttcaattttacagtagtataaaaaa60 tgtctagtgtgtatgagaataaaaatggttttaaagatggatgtatcgacttcacggcag120 gatccttgggaggtgtggcattggtgctagtcggtcaacctttggataccgtaaaagtga180 aaatgcaagctttcccaagtctttatagtggcatgacaaattgtataaaaaagacagtta240 aaagagatggatttattaaaggattatatgcaggaacaactccagctttaatagctaatg300 ttgctgaaaattctgtattattcgctggctacggagcttgccaaaaattcattgcctact360 tgactggcgaaaaagatgtatctaaattaagtacttttagtaatgcatctgtggtgctcg420 ctgcatttttttcatcattcacattatgtccaactgaattggtaaatgccactccaagcc480 ttggaggaagctcaaattcagaaggttcgctggcaaatgattgaagaatgggccgataaa540 taaccaacg 549 <210> 279 <211> 549 <212> DNA
<213> Ctenocephalides felis <400> 279 agCggCttCC aCCttCtttC gctattgtgt ccgcaaagaa tatgaaaata atatattaca 60 catagtgcat gtagtttttc taatacatga tacacttata aaataaataa taacaaacat 120 ttgaagttga tacaaaagta catcaggtta aaatgccgga aggtgtagaa aatttagttc 180 taggagaatt atatacatcg gagaagaatg gtagtgatga aacaggagat ggaactgcag 240 FC-8-C2-PCT.ST25.txt atcgccctttcaaaacaattcttcaagccatgaaacatgctggtaaggagCcatttccaa300 cgatatatgttgatgcaaaggaaccagagagtggtgagaaatatgaactcgctgccaaat360 ctcaattgaagaaaatacaaaaaatatgggtgagagatacatataaaaatgcagataaat420 taaagaaagaggaagaggatgcaaagaaacgagaacagaatctgtggaagctaaaagatt480 caaatcaagaagacaaaattaccaccgtaagtataaaattaacaggtgattcatcgaatt540 gccggttaa <210>

<211>

<212>
DNA

<213> felis Ctenocephalides <400>

cacgtgtgatttatttcttctgatttagttttgtgttgcaataaacattataatttaacc60 tcaaaacttcaaaatgtctatccgacctgtgtatcgcccgaagattgtcaagaagcgaac120 gaaaaagttcattcgccatcaatctgaccgatatgataaacttaagaggaactggcgtaa180 accaaaaggtattgacaacagagtacgtcgtcgtttcaagggccaatttttgatgcccaa240 cattggttatggatccaacagcaagacccgtcatatgttgcctactggattccacaaggt300 tttggtacataataccaaggaacttgaaatcctaatgatgcagaataggacatactgcgc360 tgaaattgcacatggtgtttcttcgaagaaacgtaaagagatcacagagagagccaaaca420 attgtcatcagagtcacaaatggcaatgcagactccgctcacaagaaaatgaataaatga480 ttgataacttcatataataaacgctaaaagtaaaaaaaaaaaaaaaaa 528 <210> 281 <211> 549 <212> DNA
<213> Ctenocephalides felis <220>
<221> misc_feature <222> (489)..(489) <223> n = unknown at position 489 <220>
<221> mist feature <222> (547)..(547) <223> n = unknown at position 547 <400>

atttagatcaaattttcagtggtattacagaaaatccataaaagcatttagataacaaac60 aattatccaacgttacctgattgcaaatcttaaactgcattaagtgataaaacacaaagc120 attctgaaataaattttgttctggggatacacatgcattcacataagttcaatggcttga180 agttacaataaagaaggctccaagggctgccaaaataattacgtgtctcgatctagcctg240 FC-8-C2-PCT.ST25.txt aatcttcgtccgctcgcctaatcatgcccaaggtcgtaaaagctgaaggggaggatcccg 300 atccaaccccatacttgttcgtatctctcgaacaaaagcgtatcgatcaaaccaaaccct 360 acgatgcgaagaaggcctgctgggtacccgacgagaaggaaggcttcgttctcggtgaaa 420 ttaaggccaccaagggggatCtCgtCa.CCgtCggCttgCCggtggagagaccaaagattt 480 caagaaggnctcgtcgccaagtgaccccccaattgaaaaatgcgaggattgtcaaatttg 540 ctacctnac 549 <210> 282 <211> 549 <212> DNA
<213> Ctenocephalides felis <220>
<221> misc_feature <222> (504)..(504) <223> n = unknown at position 504 <400>

gaaagctcaagcgaaagaagaaaaaattgctaagcagcttgaaagagagaagctacagct 60 agccttagctgctcgtgatagagctgagaagaaacaacaagagtatgaagacaagcttaa 120 atctatgcaagaagaaatggaacgatcacaggctaatttaatagaggcacaagatatgat 180 cagacgtttagaggagcaactgaaacaattgcaagcatctaaagaagaattagagcaaag 240 gcagaatgaattgcaggcaatgatgacccgccttgaggaaacaaagaacatggaagccgc 300 agagcgtgcaaaattagaagaagaaattcgtgcaaaacaagatgaagtgcaacgcattca 360 agaagaagttgaagctaaagatacagagactaggagattacagaatgaagttgatgaagc 420 caggcgtaaaaaagatgaagtagccgctgcttgttggctgcacatcaactcctcacatcc 480 catgtgatggaagaaatgacaccnagagtgtgagttccaaacggagtggagatatagctg 540 cttaatctg 549 <210> 283 <211> 550 <212> DNA
<213> Ctenocephalides felis <220>
<221> misc_feature <222> (496)..(496) <223> n = unknown at position 496 <400> 283 aagccgcatt tgttattgag aaatatatgc taaaatatat tgtctcagaa ttaatataat 60 gtgctgagtt tgcattaata ttgaaacgtt ttattcattc tgaggcatct atataaaata 120 FC-8-C2-PCT.ST25.txt aataaatatgaaagcaattttgataacattgatagtcgccgcggctgtgtattccgtaag 180 gcctgaggttttcctggaagaaaacttcgtagacgatacgtggacaaatacatgggttta 240 tagtgaacaccctggcaaagaattcggcaaattcgtgcacactgccggaaagttctataa 300 cgatgccgaagcagacaaaggtttgcaaacaagtcaagatgctaggttctacgctctatc 360 tcataagttcaaacctttctcaaataaagacaagacattagttgtcaattttctgttaaa 420 catgaacaaaacattgactgtggaggtggtacttgaaggtgtcgattgaagttgaatcaa 480 aaggacatgcatggtnaagtcctatgaaatatgtttggcctgcatttgggccaggaacta 540 aaaggtccgt 550 <210> 284 <211> 328 <212> DNA
<213> Ctenocephalides felis <400> 284 catccgttca ccaattggtg taggatccgt agctaaagtg ttcggaggac gtaaacgtaa 60 tggtgtatct ccttcacatt tccacaaatc atcaggaggt attgcacgca aagcattgca 120 agctttggaa tctttgaaat tggttgagaa acacccagat ggtggccgca agcttaccgt 180 tcagggaaga cgtgatttag atcgtatcgc tgcccaagtt agggctaaac aaagggcaag 240 aaaagatacc actggtccta ttgttttgta aaacatgttt atatataaat aaaaaaataa 300 gatctaaaaa aaaaaaaaaa aaaaaaaa 328 <210> 285 <211> 550 <212> DNA
<213> Ctenocephalides felis <220>
<221> misc_feature <222> (535)..(535) <223> n = unknown at position 535 <400>

cagcgatttcctttttattccggctaagtgagaaaacacaaacacaaccaaaatggacgc 60 gatcaagaagaaaatgcaagcgatgaagctggaaaaggagaacgcaatggaccgcgctct 120 caactgcgaacaggaagctaaggatgccaacctgcgcgctgagaaggccgaggaagaggc 180 ccgttctctgcaaaagaagatccaatcaattgagaacgatcttgaccaaactcaagagtc 240 attgatgcaagtcaatggaaaactcgaagagaaagacaaggctctccaaaatgccgaatc 300 tgaagtcgctgccttgaaccgacgcattcaactcttggaagaagatttggaacgttcaga 360 agaacgtcttgccaccgcactgctaaattggctgaagcctcacaggctgctgatgaatca 420 gaacgccccgtaaggtgcttgagaaccgttccctcgcgaggaagagcgcatggaccactt 480 FC-8-C2-PCT.ST25.txt gagaccaact taaagaagcg cgttctggct gaggaggccg ccaagaaata cgatnggttg 540 ccgtaaattg <210> 286 <211> 457 <212> DNA
<213> Ctenocephalides felis <220>
<221> misc_feature <222> (446)..(446) <223> n = unknown at position 446 <400>

gcaaacaacaaggtccgctgatgcaagatccacacgattgtcacgcatattacacatgtc 60 tagaaattggatcattaccgaaacattttaattgtaataaaggtgcttatttcaatacag 120 tcaaattaaaatgcgtgaaaggaaattgcgaaaatagcacagaaattcctcttcctgagc 180 ttccagacatttgcgatgaagtaggacctttggtgcaagatccaaacgattgccgcaagt 240 attattcatgcgtcacgattggaaaagaacctgaacattttacgtgcaataaaggggcgt 300 attttgatcgagaaagattacggtgtgtcagaggatcttgttaacaaatattgttatata 360 acaaagttcaatctttaattattatttagaagaatttgaaaatgtatatttaatgttttt 420 taataaaatagtttattggcaatttnaaaaaaaaaaa 457 <210>

<211>

<212>
DNA

<213> felis w Ctenocephalides <400>

attgtaccaccaacaataaatttagaaaatctaactgaagatatggacttaaattatgtg 60 cctaataaatcttgtgattggaagagttgtgataagagaatagctttgaaaaatgctttt 120 ggttttggaggtacaaatgcatcattatgtataggcgaatatagagagatataatcacat 180 aatgcttatatgctgattttaaaaagagataatataatagttttctaatactaataaata 240 aaatatattttaatat 256 <210> 288 <211> 550 <212> DNA
<213> Ctenocephalides felis <220>
<221> misc_feature <222> (530)..(530) <223> n = unknown at position 530 FC-8-C2-PCT.ST25.txt <400>

cgacaacctgacgcccttgaaagacaacgaaggcattctagtgccattggtgcaatcgtg60 cgatgacgaccatcctgctattttccattcgatggcaccttttccattgttacgtaaagt120 tcttgtacggttattgcattctgttgacttgatactggagaaggctaacgaaatttaccc180 taaccagtatcttcagaaggccaaatattattggggtaactttgaaatgttgacgtcagt240 aatttccccaaaacgtttgacgattttgcacgtcgattacaacggcaaaattttgggatc300 gatgcattcgatgacaggagacgtcaaaggcatttcgcatgccttacctcattccgatgg360 atatctgtatttggggtcaccatttaataactatttggcacgagtacctatgcccaaatc420 ttcgaagatcagagtgtctaaggtcacttatgagggcgagggattgatgttgctggggaa480 aaggtaaaggagactcctaaggaaagaagtacctaaggaaagaggtgccnaggaaaggaa540 gccctaggtg 550 <210> 289 <211> 550 <212> DNA
<213> Ctenocephalides fells <220>
<221> misc_feature <222> (512)..(512) <223> n = unknown at position 512 <220>
<221> misc_feature <222> (539)..(539) <223> n = unknown at position 539 <220>
<221> misc_feature <222> (546)..(546) <223> n = unknown at position 546 <400>

aatcgacccagttcttatatttgttaaaccgtttgttaaaattcgcaaaaataaaaattt60 gcaaaaaatgcctttaaaaatcaaaaaagtgttagttagcgacgctgtcgatgcagcatg120 tgtggaattactaaagcaacatggcgtcgaagtgaaatgtcgaacaggaatgacgcctgc180 ggagcttctgcaggagatccctgctcatgacggattgattgttcgctccgatacgaaagt240 aacacgcgaagtaatagctgcagctactaatctcaaggtggtcggcagagccggagccgg300 cgtggataacatcgatatacctgcagcaagcgaacgaaatatcatcgtgcttaataoacc360 agggggtaattcaatcagcgcctgcgaactaacgtgtacgttgatggggtgtctcgcacg420 tcatgtagtgcccgccggtgcgtctatggtagctggacgtgggatagaaaaatctattca480 ggaaatgaattgatggtaaacctgccgtttangtttgggaggatggattgaagttgcang540 FC-8-C2-PCT.ST25.txt cgtatnaagt 550 <210> 290 <211> 550 <212> DNA
<213> Ctenocephalides felis <220>
<221> misc_feature <222> (30) . (30) <223> n = unknown at position 30 <220>
<221> misc_feature <222> (36) . (36) <223> n = unknown at position 36 <400>

attaattggagacgaccaaatttttaatgnaattgnaacagctcatgcatttattataat60 ttttttcatagttatgccaattttaattgggggttttggaaattgattaatcccattaat120 attaggagctccagatatagcctttccacgaataaataatataagattttgacttttacc180 tccttcattaattttattactttcaagagctatggtagaaagaggagctggaacaggatg240 aacagtttatcctcccctttcttcagtaatcgctcacagtggatctagagtagatttaac300 tatttttagacttcatatagcaggtatttcttcaatcttaggagccattaattttatttc360 tacttgtttaaatatacgacctaaaggaatatctttagaccgaataccactttttgtatg420 atcagtttttattactgctttcttattacttttatctttaccagtattagcaggggctat480 tcaatattattaacagatcgaaatttaatacatcattctttgcccttctggtggaggaga540 cccaatttat 550 <210> 291 <211> 362 <212> DNA
<213> Ctenocephalides felis <220>
<221> misc_feature <222> (95) .(95) <223> n = unknown at position 95 <220>
<221> misc_feature <222> (179)..(179) <223> n = unknown at position 179 <220>
<221> misc_feature <222> (292)..(292) FC-8-C2-PCT.ST25.txt <223> n = unknown at position 292 <400>

acaagctaaagatgccaacttgagagcagaaaaggtaaacgaggaggttcgtgaactgat 60 caagaaactcggacaagtcgaagccgatttggccntgaacaagaacaacttggagcaagc 120 caataaggatctcgaagagaaagaaaagaccttgaccgccaccgagtctgaagtggctnc 180 actcaacaggaaggtgcaacaaattgaagaagatttggaaaaatctgaagaacgtgccgg 240 aagcgctccccaaattgttggagccacacaagctgccgatgaaaccaccgtntgtccaag 300 tattggagaaccgctccaacaagatgaagagcgatggcccattgaccaccaattgaagga 360 gc 362 <210>

<211>

<212>
DNA

<213> fells Ctenocephalides <400>

attaattcctatgatggccggtacattaactaaactcagtggaaatattaaatacgtgaa 60 aaaacagaataataatatttttagacaatttttgacaacaaagaattatgccacaagggt 120 ccaagcagctaagccagtggtcgaaatggatggtgacgagatgactaggatcatctgggc 180 taaaattaaagagcaactcatttttccatatgttaaggtagaatgtttgtattatgattt 240 gggtttgccacatagagatgccaccgatgataaagtaactgtggatgccgctcaagcaat 300 tttaaaacacaatgtcggcataaaatgtgcgacaattacccctgatgaggcaagagtagc 360 tgaatttaaattaaagaagatgtggctcagtccaaatggaaccattcgtaatatactggg 420 cggaacagtgttccgtgaacccattctttgtaaaatatacctcgtcttgacctggatggc 480 taaacctattataatagccgcacgcgcatggtgatcaatataaagctaagatttgagtaa 540 atgacctgg 549 <210> 293 <211> 549 <212> DNA
<213> Ctenocephalides fells <400>

tataaattgtatatatgagcaatgaatcaatttttttaaacattttgattttcctgggcc 60 ctgtagcaaacttgatatttgacaactgaaccatcaaagctatatataaatattaatcga 120 atttctataaatcttaaatacatattttgaattaaagatagtaaattaacaatggcagcg 180 ggggctgagcctttgtcactagctaaagatgtcaaaagagcctgcgagctccttgataaa 240 ttacaaatgactggagaaataccagccacaaaaatagctgccttacaaaaagttttacaa 300 tccgatttttttactgccgttcgcgaagtttatgaacatatttatgaaacagtagatatt 360 FC-8-C2-PCT.ST25.txt caaggttctg aagacataag agcatcagca acagcaaagg ctactgttgc agcttttgca 420 gccagtgagg ccatgccatc cacgagttgt tgaattccta agacagatga aggcttaggg 480 tttaatgtat gggtggaaaa gaacaaaatt ctccatatca tatcaagaat atccagtggt 540 gagctgtcg 549 <210> 294 <211> 549 <212> DNA
<213> Ctenocephalides felis <220>
<221> misc_feature <222> (474)..(474) <223> n = unknown at position 474 <220>
<221> misc_feature <222> (485)..(485) <223> n = unknown at position 485 <220>
<221> misc_feature i <222> (515)..(515) <223> n = unknown at position 515 <220>
<221> misc_feature <222> (517)..(517) <223> n = unknown at position 517 <220>
<221> misc_feature <222> (532)..(532) <223> n = unknown at position 532 <400>

gcttatgtttgtttttattttgacgttgctaaaattttagctttaatgtttgttttcttg60 tgataaattagttttattttaaaggctagcgcataataataatgatgcatctgagcgctg120 acatatctagtgcacttcagcaacttgagagcatcaagacagcaatagatgactcccatg180 atccaaaacttcagctcagtactaatgaagatttggatatgataataagcctattgcaag240 atccagtttttcgaagcattgttactactcaagattcactaggtgaattgaattcccaaa300 taacacaacatccatcaatattaccaggagattttgatataactacttcaggtgatctaa360 ttctgcggtgcccccttctcttgatttatatgataatgagtacactgatgaacaaagagt420 accctctgacaattaagtccaggtagccctcagaggttaggtatagcatcggtnggggca480 gtcangggaacattacattcatgaagggatcaatntngcaatgaggcaaccntgatggat540 FC-8-C2-PCT.ST25.txt attcccaca 549 <210> 295 <211> 512 <212> DNA
<213> Ctenocephalides fells <220>
<221> misc_feature <222> (30) .(30) <223> n = unknown at position 30 <400>

atcagactgaacgagccttccagaagcagntgggtgtaaacctcaaccgcaagatcggtt60 taaaaaagaaatccttacgtcttcacaggaatgtaggattaggattcaaaacaccccgtg120 aggctattgaaggttcttacatcgacaaaaaatgcccattcacaggaaatgtatctatca180 gagggcgtattttgacaggtgttgtacaaaaaatgaaaatgcagcgcactattgtcattc240 gacgggattatctgcattatattaggaagtacaacagatttgaaaagagacatcgcaata300 tgtctgtgcacctttcaccatgcttcagggatgttgaaattggtgatgttgtgaccattg360 gtgaatgcagacccctttcaaagaccgttcgtttcaatgttctcaaagtgacaaaaggaa420 caggttctaaaaagagcttcaagaaattttagtaatctttattacaataaacatttgttc480 ttgtaaaaaaaaaaaaaaaaaaaaaaaaaaas 512 <210> 296 <211> 549 <212> DNA
<213> Ctenocephalides fells <220>
<221> misc_feature <222> (514)..(514) <223> n = unknown at position 514 <400>

ggctcttggtcgaagaggctgttaacgatgataacagtgtggtggctttgtctcaagcta60 aaatggacgaactgcagctattcagaggcgatacagtattattaaaaggcaaacgcagga120 aggagacagtttgcatcgttctctctgatgatacttgccccgatgaaaaaatccgtatga180 acagagttgtacgtaacaacttgcgtgtccgcttgtctgatgttgtttctgtgcagcctt240 gtccagacgtgaagtatggaaagcgaattcatgtgctgcccattgatgattctgttgaag300 gcctaacaggaaatctgttcgaggtctacctcaagccctacttcttagaagcctatcggc360 caattcacaaggacgacacatttattgtccgtggcggtatgagagcaatagaattcaaag420 tagtcgaaacagatctgcttcttattgatcgtggtcctgcacagtcattcattgcgaagg480 FC-8-C2-PCT.ST25.txt ggatccaatc aagcgtgagg aagagaagag ctcnatgctg cggtatgatg atattgtggt 540 gagaaacat <210> 297 <211> 296 <212> DNA
<213> Ctenocephalides felis <220>
<221> misc_feature <222> (102)..(102) <223> n = unknown at position 102 <220>
<221> misc_feature <222> (121)..(121) <223> n = unknown at position 121 <400>

cctaataaattaggaggagttattgccttactattatcaattttaattattattatttta 60 cctattaataataaaatatttttaagaaattcattttatttnttaaataaaattttattt 120 ngatttttcatttatattttaattttactaacatgaattggagctcgccagttgaagacc 180 ttatattattcaggacaaattcttacaattctatacttttcttattatttaattaatcca 240 tatatttattataaatgagataatttattattataaaaaaaaaaaaaaaaaaaaaa 296 <210> 298 <211> 549 <212> DNA
<213> Ctenocephalides felis <220>
<221> misc_feature <222> (505)..(505) <223> n = unknown at position 505 <220>
<221> misc_feature <222> (535)..(535) <223> n = unknown at position 535 <400>

ggaacatttattcgtgaagctagcactagtcatgcattgtttggtaaacatattctcact60 gtagacatgttcaacaaagaacaattaaatgatattttca~atttggctgaaaatatcaaa120 gctcgcgttgtcaaagatcgaccagtagatgaaattttgcgcggtaaggtaatggcttct180 atcttttatgaagtgagtactcgtaccagctgcagctttgctgctgcaatgcagaggctc240 ggaggccgagtgatccatatagatgaaactagctcatctgctaagaaaggcgaaactcta300 gaagattctgtttctgtcatggctggttactcggatgtaattgtacttcgccatccagaa360 FC-8-C2-PCT. ST25 . tact ccaggagcag tagctaaagt ggctgcgcac tgtagaaagc cattaataaa tgctggcgat 420 ggagttggcg aacatccaac tcagcgtgct tgatatattt actattcgtg aagaaatggt 480 ccgttatggt tgactatact atggncggac ttgaaaatgc gtctgacctc attgntcgtt 540 ggtgcgtat <210> 299 <211> 549 <212> DNA
<213> Ctenocephalides felis <220>
<221> misC_feature <222> (540)..(540) <223> n = unknown at position 540 <400>

cttcaaacctttaatgttttgaaacacaaactgttcataatcaattgtagttttgtgata60 aaactcaatatgatcgtataaacctgtgtacctagaaaaaacagaggagcagggagggag120 tggacattttattctggatttccttgatggtgtacctataaaattttccttagtactgat180 tggggaactgggaatgggaggcgtgtgaatttgaactttagtagaggaagtacaaggatg240 tggcgataaacttacgtgggaatgtttgtgaagacgatctgatatggaaggagcagaggt300 aaaagatgagcaagcagaaagtggatatcttagtttctcttttgttgatggtgtacctgt360 aaaattttccttagtactgattggggaactgggaatgggaggcgtgtgaatttgaacttt420 agtagaggaagtcaaggatgtggcgataaacttacgtgggaatgttgtgaagacgatctg480 tatggaaggagcagagtaaaagatagcagcagaagtggatatctagttctctttgtgtgn540 gtcctgtaa <210> 300 <211> 549 <212> DNA
<213> Ctenocephalides felis <220>
<221> misc_feature <222> (537)..(537) <223> n = unknown at position 537 <400> 300 cttaagtgtt cagaatgcca caatcaatgc atgtaaatgg ctatgccaac ggccacagcg 60 gttccttcga tatggaggat ggatctgttt tcttgtttac ttcggaatct gttggcgaag 120 gacatccaga taaaatgtgt gatcagatca gtgatgctgt attagatgct cacttgaagc 180 aagatccaaa tgctaaagtt gcttgtgaaa ctgttactaa aactggaatg gttctcctct 240 FC-8-C2-PCT.

. tact gtggagaaataacttccaatgctgtagttgattatcaaaaagtggttcgtgacacggtga 300 aacacattggttatgatgattcatctaaagggtttgactggcgtacccttaatcttctgg 360 ttgcattggaacagcagagtccagacattgcgggtggtgtacacatgaataggcaagaac 420 atgacataggtgctggggatcaggtttcgactataggacctgcaatgtctacttgcattg 480 atctcagcacctatatagcagctggagtcatgacatcgaaggatgaaaaatgggcangaa 540 acagggtta 549 <210> 301 <211> 475 <212> DNA
<213> Ctenocephalides felis <220>
<221> misc_feature <222> (87) .(87) <223> n = unknown at position 87 <220>
<221> misc_feature <222> (95) .(95) <223> n = unknown at position 95 <220>
<221> misc_feature <222> (132)..(132) <223> n = unknown at position 132 <220>
<221> misc_feature <222> (181)..(181) <223> n = unknown at position 181 <400>

gttttggcaatgtcgcaccgtaagttttccgggccgagacatggctccatggggttctac 60 cccaagaagaggtcccagcgtcaccgnggtaaagnaaaggctttccctaaagatgaccct 120 agcaaaccagtncatttaactgcctttattggttacaaagctggtatgacccacgtgctt 180 ngtgaggcagacagacctggatcaaagatcaacaagaaagaaattgttgaagccgtaact 240 attttggaaacaccacccatggtcgtggttggagctgtcggttatattgaaactccacat 300 ggtctacgggcattggttactgtttgggctgaacatttgtcagaggaatgccgccgtaga 360 ttttacaaaaactggtataagagcaagaagaaggctttctctaagtcctcaagaaatggc 420 aagatgagcttggtcgcaaatcaattgaaagggattgaagaaaatggcaaatctg 475 <210> 302 <211> 549 <212> DNA

FC-8-C2-PCT.ST25.txt <213> felis Ctenocephalides <400>

gtgtattcatattaaaaactgctgaatccacgtgtgtataatgtcagacgcagtggtggc 60 aggaagtggcggacaacctaattctggacaaatcaagaaagaattatcggaaggcattat 120 ataccagaccgcagaaagtccaggatcaacatgctgcacagcgctgtattccgatgtaga 180 agtgataaagatggaaatttcggatcattttgaagttgttgacccgaaatctctacagat 240 agcaaatagtccgggtagtccagatagacaattctgcagctcaaccacggcgtcaattgg 300 ggaaattacgacaaacgatgatattaaagaagacagtccaaggcgtctttgccttgtttg 360 tggtgatatagcctctggattccattacggcgttgcctcatgtgaggcatgcaaagcatt 420 ttttaagagaacaatacagggtaacatagagtataattgtccggcagttggagattgtga 480 aatcaataaacgaaggcaaaagcatgtcaagcctgtcgattcaaaaatgttagcattgga 540 tgtaaaaga 549 <210> 303 <211> 549 <212> DNA
<213> Ctenocephalides felis <220>
<221> misc_feature <222> (541)..(541) <223> n = unknown at position 541 <400>

gccgtcaaaaatggctggagtattatttcgtgttaatttggctaaaacacaattgggcat 60 aatcagcccagtttttacaattagacataacagcggtggaccaccaccaccaggcactcc 120 acctccacaaacacgtactaaatttggtcctcttaaagatgaagacagaatttttacaaa 180 tctttatggacgtcacgattggagattgaaaggatctttgaaaagaggagattggtataa 240 aactaaagaaatcattcttaagggagctgattggatcataaatgaaattaaaacttctgg 300 actcagaggtcgtggaggtgctggttttccttcaggtttgaaatggtctttcatgaacaa 360 accaggagatggaaggccaaaataccttgtggttaatgctgatgaaggagagccaggaac 420 atgtaaagatcgtgaaataatgcgacatgaccctcataaattggtggaggctgttaattg 480 caggaagagcatggcgctcgactgctcattacatagaggagaatttatatgaacatcaat 540 ntcagttgc 549 <210> 304 <211> 406 <212> DNA
<213> Ctenocephalides felis <400> 304 FC-8-C2-PCT.ST25.txt gttaatttaaaataacaaaatgaaaggaacattattaatattatcatgtcttgtgatcat 60 gataagtgccgaatatgctgacgtagatgtgtgccaagatttggacgatggaacttttct 120 tgctgattcaaacaattgccaaaatttcttcatttgtgatggaggccgagcttggaaaat 180 gtattgtccaggatcacttttatggaatgatcacgaaggaacatgtgattacgcacaaaa 240 tgtagaatgttaccaaccagaataaaacattttaatatctgacagcgattttctgaaact 300 atatttcatactactgttataataaatttatcttcattgctCtCCtCCtataaatttatt 360 ccgttttaataaaatcaatataaagacaaaaaaaaaaaaaaaaaaa 406 <210> 305 <211> 549 <212> DNA
<213> Ctenocephalides felis <220>
<221> misc_feature <222> (524)..(524) <223> n = unknown at position 524 <220>
<221> misc_feature <222> (539)..(539) <223> n = unknown at position 539 <400>

ctgataccatcgaagccagtgctgattcatgacttgggtcgtgtttatatacaaactggt 60 cctgatgttaagaggtgcattcttagattattagagggaccagttagacaactcggcatg 120 gaaaatactgaattgatgaaattagttgaggcctgtgcaaagggatctgaaaccttggtt 180 acaagggtgatacatattttgaccgaaagaagtttgcctagcatggaactagtttctaga 240 gttcgagatctttatcacactaaagtatctgatgtccgattcttaataccagtattaaat 300 ggtcttaataaagacgaggttatagattctttgcccaaattcattaaactaaatccagta 360 gttttaaaggaagtttttaataagctgctgaataatcaagcaggtcctacatcatatcct 420 agtccagttacacctatagagttgttgtagcgtacatacaatagacacaactagtgcaga 480 tttaaaattcgagtgaaagcaacaagttgtgttagcagagaagnaattacacccagaang 540 atgcagagt 549 <210> 306 <211> 534 <212> DNA
<213> Ctenocephalides.felis <220>
<221> misc_feature <222> (4). (4) <223> n = unknown at position 4 FC-8-C2-PCT.ST25.txt <400>

tgangatcggcttgtgcgcgtacagtggatacaaaatttatcccggccatggcaaaacca60 tggtcaaaattgatggaaagacattcactttcctcaattcaaaatgtgaggctgctcatt120 taatgaagaggaacccacgtaaagtaacatggactgtcttgtacagacgcaagcacaaga180 aaggtcaagaagaagagttgactaagaaacgtactcgtcgtacacagaaattccaacgtg240 ctatcgtaggagcttctcttaatgacatccttgccaagaggaacatgaaacccgaagtac300 gaaaggcacaaagagaccaagctatcagggctgcaaaagagcagaagaaatccacgaaag360 cagcaaagaaggctgcagctccacctaaagtgaaagccccaccaaaagctaaggccgcta420 aagtgagccagaaagcacccccagggttggcggtaaacgataagtggtgttggagattat480 tctaatgtattatatgaaaaataatttgtaaaatgctaaaaaaaaaaaaaaaaa 534 <210> 307 <211> 549 <212> DNA
<213> Ctenocephalides fells <400>

tgaaaaactacgatccccagaaggacaagcgtttcagcggcaccgtaaaattgaagcaca60 ttccacgaccaaaaatgcaagtatgtattttgggagatcaacagcattgtgatgaagcta120 aggccaacaatgtcccatggatggatgccgaagctctgaaaaaacttaacaagaacaaaa180 agcttgtgaagaaactagctaaaaaatatgatgctttcttagcttctgaatctttaatca240 agcagatcccccgtttgttgggtccaggtttgaacaaggcaggtaaattccctggtctct300 tgtctcatcaggaatccatggtgcagaagattgatgaagttaaaggaactatcaaattcc360 agatgaagaaggtgttgtgtttatctgtagctgtggtcatgttgatatgtctcctgacga420 gttggccaaaacgttcacttgtcaattaacttcttggtgtcacttttgaagaagcattgc480 agaatgttaggtctctcatgtcaaatccactatgggcccccaaagatatataatacataa540 tgatatgtt 549 <210> 308 <211> 549 <212> DNA
<213> Ctenocephalides fells <220>
<221> misc_feature <222> (472)..(472) <223> n = unknown at position 472 <220>
<221> misc_feature <222> (524)..(524) FC-8-C2-PCT.ST25.txt <223> n = unknown at position 524 <400>

gcaagttagggatcttgatattaacactacagaaagacttaaaggtgttatggatttagt60 atttgaaaaggctgttgatgaacctagtttttctgtagcttatgcatatatgtgtaaaga120 gttagctcttatccaagttcctgctgaccaacaaaacggtcaatcttcaactgttgaata180 cgtcaatttccgtaaattgctcttatcccgttgtcaaaatgaatttgagaaaaatacagt240 agatgaaaccgcaagagaagttaaactgaaagaaattgaagcgtgtacagaccctgagaa300 gaagaaggaactacagtttaatttagaagaagaagagcgaaagattcgtgttaaatctgt360 tggaaatgttagatttattggagagttattcaaacaaggtatgttgccacaaaaattatg420 atgcagtgccttaggatgctctgcagtcaattgaagaagaaagcctcgagtnctggcaaa480 ttgtgcgacattgccggatttagaaattacaaaatcaagatttnaacctctttcttaaaa540 tgcaagaat 549 <210> 309 <211> 549 <212> DNA
<213> Ctenocephalides felis <220>
<221> misc_feature <222> (503)..(503) <223> n = unknown at position 503 <400>

atttgtatttatgatttagatttatagtgcattaagatttgtgcttcaaaaatgttatca60 cgtgcctcatatacttcatctgtattcttaaattcgctgcagcaatttcgtttacaaagc120 acattagtaattgctgaacattcaaatgcaaaattaaacccattgactcaaaatgcttta180 acagctgcatcaaaaattggaggtgaagtaacagttttaattgctggcaaaaattgcaaa240 ccgcttgctaatgaagttgccaaagcagcgggtttgaaacaagtcttattagcggaaaat300 gctgcatttgaaaactgttctgctgaagccttgacaaaacttgttgttgcagttcaagag360 caatataaatttacgcatattattggtggtgcaagttcccttggcaaggctgtattgcaa420 gaatagcggcaagttagatgtttccctatttctgaaataattgatgttaaagatctgaac480 attcgtcgacaatttatgccggnacgctttcagacactgaggtgaaagatccctaaagct540 atcttcgtg 549 <210> 310 <211> 549 <212> DNA
<213> Ctenocephalides felis FC-8-C2-PCT.ST25.txt <400>

gcccagtgcacgaggagcatgaggaaccagatccaagaagaactgagcgcttcgatgcaa60 tacttggccatgggggcgcatttctcaagagacactgtcaacaggccaggatttgctgag120 atgttcttcaaatcggcaagcgaagagagggaacatgccatgaaactcatgtcttacttg180 atgatgagaggagaactgaccgagaggctgcaggacttgatcagaacaccaactgttcca240 atcacgacttgggctgatggtttgagtgctttgaaagatgctctgaaattggaggcttcc300 gttaccaagaagattaaacatgtgatcaaagcttgcgagaacgataatggagctaatgat360 tatcatttggttgactacctgaccggtgaattcttagaagagcaatactctggtcaacgt420 gacctgccggaaagatctccaccctgggcaagatgatgaaccaacaaggtgttctcggga480 gttcttgttgacaagaaactctgggttaaatgaaacatacatcccatcaaatatcacagt540 aataaaata 549 <210> 311 <211> 548 <212> DNA
<213> Ctenocephalides felis <400> 311 aaaaacaaaa ttatttgatacattttaatctagaatcatctgtgtggctaaatattattt60 gtgtttcaat ttcgactatttgacgaaaatagataatagaaatggcacctaaagcatttg120 gtgatgtgaa aatgcaaggacaagcatataaagataaaagtaaaccggctgatattcgta180 gcagcaatat atgtgctgcgaaagctgtttctgatgcagtacgtacaagtttgggaccca240 ggggtatgga taagatgatccaagcctcaaacggtgaagtgacaattaccaatgatggtg300 ccacaatttt gaaagaaatgaatgttactcatccagcagctaagatgttggttgaattgt360 ctcgggcgca agatattgaagctggcgatggcacaacatcagttgtagtagttgcaggtg420 ctttgctgga ggctgctgaaaagttacttcacagaggacttcatccaactgcatttctga480 tgcatttcaa agatgtgctccaaagctgtggaaattcttacacctgtcacaccaattgac540 ttactgtc 548 <210> 312 <211> 549 <212> DNA
<213> Ctenocephalides felis <400> 312 tgtgtattat tcgcacacgt gaaataaatc atatcggtaa acttgtaata tctattttat 60 taactaataa gcgtagtttg tgtaataaca agtaatttct attagtagtc ataagtaatt 120 gaagagaaaa ctattttcca tataagaatt gaaagcataa aatgattctc tccgtgctga 180 aaaaatctcg catcgtgcac ttgtgttttg ccatttcatt cttcacatca ggcctgatca 240 FC-8-C2-PCT.ST25.txt taaacattgcacaattcatattatacacatgtctcaagcctttcaacaagaggctttaca300 gaaaacttggttattatttgtgctacacattttacagtcagatagtgtttttagctgact360 ggtggtccaaatctaatctgaccttacacatatcaaagaaagattatgaacaatgtggga420 aggaacatggtcttttaattatgaatcatacgtacgaaacagattggtattaggatggat480 gtttacggaaaaaattggtgtcttggaattgtaggatatgcaaaaagactattcaataca540 tccgcatta 549 <210> 313 <211> 549 <212> DNA
<213> Ctenocephalides felis <220>
<221> misc_feature <222> (529)..(529) <223> n = unknown at position 529 <400> 313 aaaaaatatt attctaacattatggcgcagatacctgcacgaccgaaaat tttgttaact60 catccggata taccgaaggaagctattgacatcatgtctcccaattgcga aataattatg120 tgcaccggac gtccgtctccatcacgacaaggaattttggaaaaaattgt gggagttcat180 ggtttaatgt ggtgtactaaagaacgtttggacaaagagatattagatgc tgcaggaccc240 aacattatgg ccatatctactatgtcggctgggctggacaatgtagattt acctgaaatc300 cgcaaaagaa atattccaattggttatactgcaggagttctgaataatgc tgttgctgat360 ttaactgttg ggcttatgatagctactgctagaagatttggagaagctag gaaacacata420 gagaatggaa catggggaagcggacctgcgtggtttttaggcagaaatgt atctgaaact480 cagctggtat tgtcggctaggagaaattggcaaactgtgcaagaagatng aaagcttcaa540 tatgactat 549 <210> 314 <211> 549 <212> DNA

<213> Ctenocephalides felis <220>

<221> misc_feature <222> (532)..(532) <223> n = unknown position at 532 <400> 314 gtggtgcagg atttgtagat ggtatgaatc ttggagataa caccaaggca gcagttattc 60 gtcttggttt aatggaaatg attcgttttg ttgatgtttt ctatccagga agtaaacttt 120 ctactttctt tgaatcttgc ggtgtggctg atttagttac tacatgttat ggtggtagaa 180 FC-8-C2-PCT.

. tact atcgtagagtttctgaagcatttgtgaaaacaggtaaatcattgaaacaattagaagacg240 aaatgttaaatggtcaaaaattgcaaggaccaataacagcagaagaagttaattttatgc300 tgagaaatcgtggcatgcaagacaaatttcctttgttcactgctgttcataaaatttgca360 caggagatatggatgttaaagaatttcttaattgtataaggagccacccggaacacatgt420 aagtgcctgctttataaaatataaatcagatttaaaatgcttctcagatggtaatattag480 taatgtctttacatgaacataagtgagagttgctagttgtttgatattttgntcttcata540 tagtatttg <210> 315 <211> 550 <212> DNA
<213> Ctenocephalides fells <220>
<221> misc_feature <222> (365)..(365) <223> n = unknown at position 365 <220>
<221> misc_feature <222> (409)..(409) <223> n = unknown at position 409 <220>
<221> misc_feature <222> (427)..(427) <223> n = unknown at position 427 <220>
<221> misc_feature <222> (441)..(441) <223> n = unknown at position 441 <220>
<221> misc_feature <222> (447)..(447) <223> n = unknown at position 447 <220>
<221> misc_feature <222> (457)..(457) <223> n = unknown at position 457 <220>
<221> misc_feature <222> (543)..(543) <223> n = unknown at position 543 FC-8-C2-PCT.ST25.txt <400>

cttgtgccggtccgagagcaccctaaattcaactttgttggaaaactgttgggacctaag60 ggtaattccatgaaaagattacaagaagatactatgtgtaagatggccgtcttaggtaga120 ggctcaatgaaagacagacaaaaggaagaagaacttcgtaattccttagatcccaaatat180 gctcatttggctgatgatctacatgttgaaatatcggctttgggtcctccacagaagctc240 acgctcgagttgcttatgctttggctgaagtccgtaatatttggtccctgataataatga300 tacaattcgccaagaacaatgcgtgaaatgatgactgacccaatgcccgccagaagaaat360 gagangagcacccacattagacgaggtggttggctgtggtgcttctcgngaggaggatac420 taatcgngcagcttcgacatntggggnatgcttctgngttctaatgtgatccggcctcac480 ccctcctcactcgtgtatcctgtaactaaatcttcattaataacaaagtgctgagaactt540 gtntaaaccc 550 <210> 316 <211> 550 <212> DNA
<213> Ctenocephalides felis <220>
<221> mist-feature <222> (33)..(33) <223> n = unknown at position 33 <220>
<221> misc_feature <222> (406)..(406) <223> n = unknown at position 406 <400>

tcttattcatattaattataagaacatttattngtatttcttcaaattcttgattaggag60 catgaattggattagaaataaatttattatcatttatccctatactaaatgataataaaa120 atttactatctaatgaatcttcattaaaatattttctagttcaagtttttgcttcaattt180 tattattattttttatttctttgaattttttatttaaaaacttttttagaataatatatt240 ttaatgaaatttatttaattttattaaattcatccctatttttaaaaataggggctgccc300 catttcacttttgatttcctagaataatagaaggaataaattgaataaataattttattt360 taataacatgacaaaaaattaaccctataatttgttaagttattgnattaatataaatta420 ttttatttaattcattattaagaattataattggagcttaggtggattaaataatcctct480 tacaaaaattatacttattctcaatacactatggctgaataatattgtttaataaataat540 gaataatttt 550 <210> 317 <211> 474 FC-8-C2-PCT.ST25.txt <212> DNA
<213> Ctenocephalides felis <220>
<221> misc_feature <222> (4). (4) <223> n = unknown at position 4 <220>
<221> misc_feature <222> (456)..(456) <223> n = unknown at position 456 <220>
<221> misc_feature <222> (467)..(467) <223> n = unknown at position 467 <400> 317 gtcncattggtgccttccaaacccatgtgtgtcgaatccttccaggagttCCCtCCattg6O

ggtcgtttcgctgtgcgtgacatgagacagaccgttgccgtcggtgtcatcaagtctgtg120 aacttcaaggatgcctccggtggtaaggtcaccaaggctgccgaaaaagccaccaaggga180 aagaagtagctagatctaccgatctgcttactgcagatgttcaacactgtaatgaaacac240 tacttccatcgcaaagcgtttcgaagaaaaaaggcctcattcattcctttactatatgtt300 ttttgactcagctttatattttatatgactattttatagaataataattattttatgttc360 tgctctatattaaaatgaatttatattaatgtatggatattatgtgctaagaaagaactt420 gaagagatttgcagctgatgtgtattatagtatggnataaatggaanccaaagt 474 <210>

<211>

<212>
DNA

<213> felis Ctenocephalides <400>

agtttgctacacacagcacgtgtaaattttcataaaataaattaaaaatggataaacctg60 tagttcttgctcgcgttatgaaagtcttgggccgtacaggatcccaaggacaatgtacac120 aagttaaagttgaatttattggtgaacaaaaccgacaaatcatccgaaatgtcaaaggac180 cagtacgagagggtgatattttaacacttttggaatctgaacgtgaagctagaagactaa240 gatagattaattctaaaatgtgagaggtgatatcctgaactataatatctgggttcaatt300 atttgtatggaattataaatatacgacttatcactcccaaaaaaaaaaaaaaaaaa 356 <210> 319 <211> 550 <212> DNA
<213> Ctenocephalides felis FC-8-C2-PCT.ST25.txt <220>
<221> misc_feature <222> (537)..(537) <223> n = unknown at position 537 <400>

aaataatctctgaaaatgttgaagggcgttgtaggtcttgttactggaggtgcttctggt60 ttgggaaaggccactgtagagcgaattgtgcaacaaggtggccgagttgttttatgtgat120 ttacctaaatcaagtggatctcaagtagcaaaagatattggagataattgtttatatgca180 ccagttgatgtcacatctgaaaaagatgttgaggaagcaatagcattaactaaagaaaaa240 tttggtcgtcttgatgttgctgtaaactgtgctggtatcggtgtagcatttaaaacatat300 aatttcaataagcagttgcctcataaattagaggatttcacaaaagttttaatggttaat360 actgttggaaccttcaatgtaattcgattgctgtggattgatgggagtaaatgaacctaa420 taaggatggtcaaccaggagtgattgtaacacacaagtgtgctgcttatgatggcagatg480 ggacaagctcttattctgcgtccaagggctttgtggaatgcactgcttttgctaganatt540 accaggcaag <210> 320 <211> 550 <212> DNA
<213> Ctenocephalides felis <400>

gattcagctttgcatagaattgtagatgcaactgatgcaattgttagcactgcatattcc60 cataaaagaacatttattatggaagtaatgggacgaaactgcggatatttagctgttgtt120 gcaggcttgtgtgttgaagctgattttatatttgcgcccgaagatcctccagatgccaac180 tggccaagtgttttatgcgcactattaagtcaggaacgattagctggaagaaggcaaaat240 attatcatggtatcagaaggggcaattgatagaaatggagaacctataacagcagaaaaa300 attaaagaagtcattattgcaggtttaaatcaggatacaagaattacagtgcttgccatg360 tacagagaggtggaagtccttctgcttttgatcgattactgggatgtcgcatgggggcag420 aagcagtattagcccttatggaagctcatgatgagtctgaccttgtgttgtgaccttgcc480 cgaaccaacagtcgtcttcaatgatggaatgtgttctcaccaaactgtacttacgcttgg540 aaaataaaat 550 <210> 321 <211> 550 <212> DNA
<213> Ctenocephalides felis <400> 321 gtcaaacatc acaaaaatat atagctttat gtgtcacaaa tgattacact tttatcgtta 60 FC-8-C2-PCT.ST25.txt tgatataatacaaaataaatacaatttcttacatgctattattataattcaatattaaaa120 catttgtactataatataacaatgaatttcttatatttattaatatgctctctttgtgcg180 gtagtaaatgccggtggggatacattagttttattagacaatcttgcaattaaagaaact240 cattccatattttttaaaagcttacaagatagaggttatgttctaacattcaaattggct300 gatgaagccaatttagttttatctaagtatggagaatatttatacaagcatttaattcta360 ttttcacctgctgtggaagagtttggaggatcattaagtgtcgaggcaattcagaattca420 tagatgaaggtggcaatgttttggtagcaggaagtgaatcctggtgatgcaattcgtgaa480 tagctctgaatgcggtttgaggagatgaggaaggacactgtatggacatcttaatatgat540 ggtctgataa <210> 322 <211> 546 <212> DNA
<213> Ctenocephalides felis <220>
<221> misc_feature <222> (28) .(28) <223> n = unknown at position 28 <220>
<221> misc_feature <222> (488)..(488) <223> n = unknown at position 488 <400>

tttttttttttttttttatttgcattgngatagccggccgattgcattcagttctaaaat60 gccgatcaaaagtatcaaagctcgtcaaattttcgactctaggggcaatcctaccgtcga120 agtcgatttagtgaccgaattaggattatttagagcagcggttccttcaggtgcttccac180 aggagcttatgaggctcttgaattacgtgataatgataagactcaatatatgggtaaagg240 agtatccaaagcattgcacaatattaatcaattgattgcccccgagttaattaaacaatc300 ttttgaggttactcaacaggaagagatcgacaaatttatgttgaaactggatggtactga360 gaacaaatctaaatttggagccaatgctatattaggagtttcattagctgtgtgcaaagc420 tggtgctgctaaaaagggtgttcctctgatcaacatattgcagatttggctggaaataaa480 aaaaatgngcttcctgtccactttcaatgtatcaatggcggagcatgctggaataactgt540 ttcaga 546 <210> 323 <211> 550 <212> DNA
<213> Ctenocephalides felis FC-8-C2-PCT.ST25.txt <220>
<221> misc_feature <222> (545)..(545) <223> n = unknown at position 545 <400>

cagaagctcaagccagagccgaagaagtcgaggaaatcaggaggaaatacagtgccagaa 60 tccaagaacaagaggagcacatcgagaccttgttggtcaagatcagcaatctggagaaac 120 agaagagcagactgcaaagcgaagttgaggtcctcatcatcgatctggagaaagcaaaca 180 gCgCtgCCCgcgatttgcagaaacgttgcgagcaattggaacgtgtcaacatcgagatca 240 aatcacgtcttgaggagaccattcagttgtatgaaggagcccagagagatttgagaacca 300 agcaacaggagttgcagagggtcaaccacgaattggacaagaccagggaacagaaagatc 360 agttggccagggaaaacaagaaattgggagacgaattgggagatgctcgcaaccagttgg 420 ccgaatacaacagacgtttgcacgaattggaactcgaattgagaagactcgagaatgagc 480 cgaagagttgctgctgcatcaagaggccgaactgtcgcaaggccaagacacgttccacgt 540 tggcnacgat 550 <210> 324 <211> 550 <212> DNA
<213> Ctenocephalides fells <220>
<221> misc_feature <222> (5). (5) <223> n = unknown at position 5 <220>
<221> misc_feature <222> (522)..(522) <223> n = unknown at position 522 <220>
<221> misc_feature <222> (527)..(527) <223> n = unknown at position 527 <220>
<221> misc_feature <222> (535)..(535) <223> n = unknown at position 535 <400> 324 gcaantcgca aacgagtttc agagcacagc ggctgcgcat ctgttaagct caacggctat 60 agtttttcag tattataatt tgtattaagt cgaagtaaat aacaaacatg agggaaattg 120 tgcacattca aaccggacag tgcggaaacc aaattggagc taagttctgg gagatcattt 180 FC-8-C2-PCT.ST25.txt ctgatgaacatggaattgacccaactggagcttatcttggagatcatgaacttcaattag 240 aacgtatcaatgtttattacaatgaggcttctggtggaaaatatgttccacgtgccattc 300 ttgtggatttggaacctggaaccatggactccgtgcgttctggaccatacggtcaaattt 360 ttaggccagacaattttgttttcggacaaagtggtgctggtaacaactgggctaagggac 420 attatactgaaggtgctgaactagtcgactctgtttggatgttgtgcgtaaagaagctga 480 atcttgtgattgctgcaaggttccactacccattcttgggangtggnctgcttgnatggg 540 acactattga 550 <210>

<211>

<212>
DNA

<213> felis Ctenocephalides <400>

cagcaactcatttaatatttgtggatcctgatgcgaaaaatgaaacatggatattacaca 60 cacatattgcagcagtagaacgattaccgttgagtactacaggatgcccacttcaaattc 120 gatgcaaaacattccaatcagtaatatttcttataagaaaagaaaacgatagccatgacg 180 tttataatactttgttgcaattagcccaacctgtttctatcgatgatctgtattgcttcc 240 agtacaccataaataaaaatgatatgcataagagcgaaggttggaattattttaatttag 300 aggatgaatttaaaagaatgaatgttccaaatgatgaatggatttacacagatttaaatg 360 aaaattatgagctatgtgatacttatcctaattgttgtatgtgcctgctaacagcacaat 420 aaatatgttacaaggaagtgcaaaatttcgatccaaaggaagataccggtctcacatatt 480 acacaagaacaagcttcaatatgcagatcagcaacctttctggttcagtgcacgatgtct 540 gagatgacaa <210>

<211>

<212>
DNA

<213> ocephalides Cten felis <400>

aaaattttaattttaattattatgtgttcctttatcattgatgcgagaagtaaaagtttc 60 gaaataaagttaagacggtggaaaagtcctcgacttcaattgattgaaatggatcgaaat 120 atgcgtatagcggtttggaaagatattcaagaaaacgaaagctattattcggatccatct 180 aaacctaaacctcctccaggtcctaaggacaatgatactatagcaatttataaattttta 240 gatactgaattttatgctgaggttggaataggccatcctgtaaagtatttcaaacttgtg 300 gttgacactgcatgggcagaaacatgggtggcctcgaaacaatgtgggttaaagtgtgtt 360 ggatgttggaatcttaataaatatgactctttggcatcatcaacatttcaagaaaacggt 420 FC-8-C2-PCT.ST25.txt aaagaatttt cttttggctc agcaaagaac cataacaggg tcttctcaat agaaagtttt 480 atattgccac ataaatgtta aaatcagact ttggggaagt acatgtttgc cttgcactct 540 gtttcaaaca <210> 327 <211> 542 <212> DNA
<213> Ctenocephalides fells <220>
<221> misc_feature <222> (539)..(539) <223> n = unknown at position 539 <400> s~

agctcacagaattcagggctagctttaaccacttcgacaagaaccgcacaggacgtttgg60 cgcctgatgagttcaagtcatgcctggtttccctcggctactcgataggcaaggatcgtc120 aaggcgatttggacttccaaaggattttggcagtagtcgatcccaatggaactggatacg180 tccacttcgatgcattcttagatttcatgacccgcgaaagtacagacaccgacactgccg240 agcaagtcatcgacagcttcagaatcttggcctccgacaagccatacattctgcccgatg300 agttacgcagagaattgccaccagaccaggccgaatactgcattcaacgtatgccacctt360 acaagggacccaatggtgtacccggtgctctcgattacatgtccttcagcacagccttgt420 acggtgaaagcgatttgtaaggtagaataaattttcataaacatttaagcaaacatttga480 caagtacacataaaggtaaataatgaagttatgagagttacgatcaaaattagcattgng540 ct 542 <210> 328 <211> 542 <212> DNA
<213> Ctenocephalides fells <220>
<221> misc-feature <222> (2)..(2) <223> n = unknown at position 2 <400>

gntatggaaaaagccgaaaagctgggccatcgcgttcaaaaatgtgtggttgtcgctcat60 ctgaaacgtgtcactcctggaaccagcgaggagatcaatggagtcgagactcctatgaca120 gatggccgcgaccattggtggcatgaggaaatggacgaagttgaaccagcatgttaccca180 gaatggatggctgctgaggatccattgttcatgctttatacaagcggctccactggtaaa240 ccgaaaggggttctacatactacaggtggttatctattgtatgctgccaccacatttaaa300 atggtttttgactacaagcccgatgacatctactggtgcactgctgatgtaggatggatt360 FC-8-C2-PCT.ST25.txt actggacatt cgtatgttgt ctatggacca ttagctaatg gcgccacctc cgttatgttt 420 gaagggacgc cattttatcc ggataacgat agatattggg cggtattgaa aaatacaaag 480 tgacccagtt ttacacagca ccaaccgcat aagacttgat gaagttcggg aagaccagtc 540 as 542 <210> 329 <211> 542 <212> DNA
<213> Ctenocephalides felis <220>
<221> misc_feature <222> (31) . (31) <223> n = unknown at position 31 <400>

ttgagaaacttctcaaaattgataaaaaagntgctgtagtaatggccatggatatgttgt 60 tagcaggagttgatactacatcaaatagtacagcatttttgctgtatttcctggctacga 120 atcaaaaatgccaagataaactacgtgaagaaatcagaacaattctgccaaataaggatt 180 cacatgtaaataacgaatcatttcaccatttgccgtatttgagagcttgtatgaaagagt 240 cgagtagaatattgccaataattggaggcactgttaggaaagtaccagtcgatattgttt 300 tatcaggatatcaaattccgaaggggaccgaggtggttctaagtcatcttactacatcaa 360 tgaaaagcagtcaattccctgaacctgaaaaattcatgccagaacgatggctgagcagcc 420 aggaatcagaaggatgtcctttagcaaaaaatgctcatccattttcgcacatgccttcgg 480 tttgggcctcgcactgcgtgggaaaagattgcagatttggaaatggaactttatatgaag 540 to 542 <210> 330 <211> 539 <212> DNA
<213> Ctenocephalides felis <220>
<221> misc_feature <222> (14) .(14) <223> n = unknown at position 14 <220>
<221> misc_feature <222> (29) .(29) <223> n = unknown at position 29 <400> 330 taaaaatttt aatngcttat tcttcaggng ttcatataag aatagtatta ggaggaattt 60 FC-8-C2-PCT.ST25.txt ttactataaatatattaggtattgtaggtaggtttattttaataatttctcatggattat120 gttcttctgggatattttgtctttctaatattatttatgaacgatcaggaagacgaagaa180 ttttaattaataagggtttaataagatttataccttcaataacattattttgatttttat240 tatgtagatctaatatagcagctcctccttcattaaatttattaggtgaaattatattga300 ttaattctataataagttgatcaataatattaatagtattaattataataatttcattta360 taagagcaaggtatagactatatttatttgcttatagtcaacatggaataagaagaataa420 gactatattcttgtcttctggaagggttcgagaatatttattattatttttacattgatt480 ccattaaattattaattttaaaaagagattattataattatttataaaaaaaaaaaaaa 539 <210> 331 <211> 542 <212> DNA
<213> Ctenocephalides felis <220>
<221> misc_feature <222> (2). (2) <223> n = unknown at position 2 <220>
<221> misc_feature <222> (32)..(32) <223> n = unknown at position 32 <400> 331 gnctaatcgaagtgatcgtaaaatattaaatngacagctatttgttaaaacaattgtgca60 ttatagaaaaacatttaagtgaaaattaattacgtttcagcagaaatacatcttctttgt120 aaatttggggttaataatctttcaaaatgcagatctttgtgaaaactctcacagggaaaa180 ccattactttggaggtagaaccttcagataccattgaaaacgttaaagctaagattcaag240 ataaagaaggaatcccaccagatcagcaacgtttgatttttgctggtaagcaattggaag300 acggcagaactttgtctgactacaatatccaaaaggaatcaacattgcatttagtattac360 gtcttcgtggaggtatgcaaatctttgtaaaaacattgacltggaaaaactattacattag420 aggttgagccctctgatccattgagaatgtaaaagctaaaatccaagataaagaaggaat480 tccccagatcagcagcgtttgtctttgctggcaaacaattagaagatggaagacttgtct540 ga 542 <210> 332 <211> 542 <212> DNA
<213> Ctenocephalides felis <220>
<221> misc_feature FC-8-C2-PCT.ST25.txt <222> (3)..(3) <223> n = unknown at position 3 <220>
<221> misc_feature <222> (32) .(32) <223> n = unknown at position 32 <220>
<221> misc_feature <222> (38) .(38) <223> n = unknown at position 38 <400>

gtnacattgatcggatctgttagcaaaggctnccaaantgccgaaatcgatgaatgttcg60 tgtgaccacgatggacgcggaactggagttcgcgatccagcagaccaccactggaaaaca120 gcttttcgatcaggtggtcaagacgatcggtctgcgcgaagtctggttttttggactcca180 atatactgacagcaagggtgatttgacgtggattaagctttacaaaaaggtcatgaatca240 agatgtcaaaaaggaaaatccacttcaattcaaattcagagcgaaattttatcctgaaga300 tgtagctgaagaacttatacaggacattacatctcgtcttttttatcttcaggttaagaa360 cgctatactgtctgatgaaatatattgccctcctgagacatcggttcttctagcatctta420 tgctgtcaagcccgacatggagattttcaaaaagaccaacactcttctggattttggcga480 acgatagactgtaccacaaagggtaatggatcaacacaaaatgtctaaagaagaatggga540 ac 542 <210>

<211>

<212>
DNA

<213>
Ctenocephalides felis <400>

cagacactgacaaaggcaaaaatgggtcgtcgaccggccagatgttatcgctattgcaaa60 aacaagccctaccccaaatctcggttctgtcgtggtgtgccagacgctaaaattcgtatc120 ttcgatttgggtaagaagaaggcaggcgtagaagattttccactatgtgtgcatcttgta180 tctgatgaatatgaacaattgagttctgaggcactggaagcaggacgtatttgctgtaac240 aaatacctcgttaagaattgtggtaaagatcaattccacatcagaatgaggctgcatcct300 ttccatgttatccgcatcaataaaatgttatcgtgtgctggagctgataggctccaaact360 ggaatgcgtggtgcttttggaaaaccacaaggtactgttgccagagttcacatcggtcaa420 ccaatcatgtctgtcgttccagtgacagatacaaggccgctgttgtagaggctctgcgtc480 gtgctaagttcaagttccctgcagacaaaagatctatgttccaagaaatggggattcact540 as 542 FC-8-C2-PCT.ST25.txt <210>

<211>

<212>
DNA

<213>
Ctenocephalides felis <400>

cacaagttctattcaaccagatagaatcttacttatggatacgtttttccaaattttgat60 cttccatggagagactatagctcaatggagagctttgcgatatcaagatatgcctgagta120 tgaaaatttcaaacaacttttgcaagctccagtagatgatgctcaggaaattttattgac180 acgttttccaatgcctcgttatattgataccgagcaaggtggatcacaggctcgattttt240 attatccaaagtcaatccctcgcagacacataacaatatgtatgcttatggaggagacgg300 aggagcaccagtattgactgatgatgtgtctcttcaagtatttatggagcatctaaagaa360 attagcagtatcttcaaattcataaaattatataaagacagaaatagatatacaaatctt420 attttatctgtaatattgtgtgtcagtctttatatttgcaatatagataagtaataaatt480 caattgattatgaacctaaatacattgatatattaaaagaacatttacactaaaaaaaaa540 as 542 <210> 335 <211> 542 <212> DNA
<213> Ctenocephalides felis <220>
<221> misc_feature <222> (538)..(538) <223> n = unknown at position 538 <400>

caggagaaagcttcagttcgtgcacactaaatgtgttagtaccaggcgagaagaaaaaga60 caccagctttcagcactttcccagagtccgccagcgtgcaagagggcgagagtgcatcgt120 tcttagttcgcacagaagacgaagttcttggacttcaatggataaaagatggtaaaccta180 ttgacgagaagagctctcgctatcgattcacaatggagggcaaaacgaccttccgattag240 agatagtatcctgcgccagcatagacgtgggccaataccaagccaaagccatcggtaaga300 caggagaaacattcgctgcgttctcagtgaatgtcgcagccgagcattgagccgaacaac360 taaattaataaccgatcctgcacacggcttaggccaaaaaacgagacgactcgcgttgaa420 o.

tcgcgagatatcgtgtaaacttaagtttattaaattattatgtttatatgaataaattat480 tagtgtgatctaagcgtgttttcattataattcgccttgccacttcgcttatatatangc540 cg 542 <210> 336 FC-8-C2-PCT.ST25.txt <211>

<212>
DNA

<213>
Ctenocephalides felis <400>

cagacactgacaaaggcaaaaatgggtcgtcgaccggccagatgttatcgctattgcaaa 60 aacaagccctaccccaaatctcggttctgtcgtggtgtgccagacgctaaaattcgtatc 120 ttcgatttgggtaagaagaaggcaggcgtagaagattttccactatgtgtgcatcttgta 180 tctgatgaatatgaacaattgagttctgaggcactggaagcaggacgtatttgctgtaac 240 aaatacctcgttaagaattgtggtaaagatcaattccacatcagaatgaggctgcatcct 300 ttccatgttatccgcatcaataaaatgttatcgtgtgctggagctgataggctccaaact 360 ggaatgcgtggtgcttttggaaaaccacaaggtactgttgccagagttcacatcggtcaa 420 ccaatcatgtctgtcgttccagtgacagatacaaggccgctgttgtagaggctctgcgtc 480 gtgctaagttcaagttccctgcagacaaaagatctatgttccaagaaatggggattcact 540 as <210> 337 <211> 542 <212> DNA
<213> Ctenocephalides felis <220>
<221> misc_feature <222> (33)..(33) <223> n = unknown at position 33 <400>

cgaaaccataaaaaaatacggcaaactcaatgncctcatcaacaatgccggcatgttgga 60 aacaggcaccatcgagacaaccagtctagaccagtacgacagaatcatgaacgttaacat 120 gagatctgtgtaccatttgacaatgttagcagtgcctcatttgattgagacgaagggaaa 180 tattgtgaatgtctcgagtgttaatggtatacgttcttttcctggtgttctggcttataa 240 tatatccaaagcggctttggatcagtttacaagatgcgttgctttggaacttgctcttaa 300 gcaagttcgtgttaattctgttaatccgggggtcattattactgagatacataaacgggg 360 gggaatggatgatgaaacttataaaaaattcttagaacattgcaaaactactcacgcctt 420 aggacgtccaggacaagtaagtgaagtatccaatgccattgcgtttcttgcaagcgagca 480 cagcagctttattactggtgcttcgttaccggtgatggaggcagacatgcctgtgcccac 540 g 542 <210> 338 <211> 542 <212> DNA
<213> Ctenocephalides felis FC-8-C2-PCT.ST25.txt <220>
<221> misc_feature <222> (31) .(31) <223> n = unknown at position 31 <400>

aaaaaaacaagcttttttaacgaaatatttngcaaaaagagaaatatataaacaaactta60 atgtctgaaacaatataaaataatattttcaacgctaacgcttaaataattccaaaacaa120 atcatgagtgactattttcttttatgttaacctctttacgcaaagaaaatgtttataaac180 ttcaaacaacgttaaggtgataaatgtttacctaataataataataataagtttattttg240 attttgtgtataaaaacatgtcttggaggctgctgttgaagttttaatttgcaagtgtta300 gatacaacttaataaaaaatgtctcaatgatgacaataacaaaaaattcttttacccaag360 tgcctcttgtatttataatatgatatacaaaatgcccccgtttagacgtaaaaaatctgg420 aaagtcttttccagttaaagtttgcactttggacgctgagttagaatttaatttggagtg480 gaaagcgacaggcaaagatttattcgaattagtttgccggacgattggttacgagagacc540 tg 542 <210> 339 <211> 542 <212> DNA
<213> Ctenocephalides felis <220>
<221> misc_feature <222> (32) .(32) <223> n = unknown at position 32 <400>

agttcatcttatgttgtaattatattatcagnttgttgtaaacacttcttattttatatc60 ataaatatcgctgtatttgatttacacgatggctgcaagattgaattcattgtttaaaac120 agatttcacaaattacataaaacgtttaaacagcgttcaaatacgttgcttaaatttgca180 agaatatcaaagtaaaactctacttcaaaaaagtggcgttgccgttcaagcctttagatt240 attagataacaccgaaaacacaagtgtcttaaatgatttcaaagtccccgaatatgttat300 caaggcccaagttttagcaggaggtagaggcaaaggccattttgataatggctttaaagg360 tggtgttcatataacaaaggaccctaaagaaatattgccaattgctaaaaatatgatggg420 tcacagacttattacaaaacaaacaaaagctgaaggtatacttgtcaaaaagttatggtg480 cacaaagcgttgacatttgcgggaaacatattatgtatcatcatggcagagctcatatgg540 cc 542 <210> 340 FC-8-C2-PCT.

. tact <211>

<212>
DNA

<213>
Ctenocephalides fells <400>

gagaaaagagtggaagaatttaaattaaagaaaatgtggaaaagtcccaatggaactatc 60 agaaatattctcggtggcacagtcttcagagaagcaatcatctgcaagaacataccccga 120 ttggtaacaggatggaatgagcccatagtcatcggcagacacgctcatgctgatcaatac 180 aaagccaccgacttcgtggtaccaggaaagggtaaattagaattgacatttacccccgaa 240 tcgggaagtccaatgagcttcaccgtccacacttaccaaggacctggagtcgccatggga 300 atgttcaacaccgatagttcaattgtagatttcgctaatgcctcattccaatatgcctta 360 aaccgaaaattgcccttgtacctatctaccaaaaataccattctcaagaaatacgatggt 420 agatttaaggatatattccaggaaatttatgacaagcaatacaagaaagaatatgaagct 480 gtggaatctggtacgaacatcgactgatcgatgacatggtcgctacgcaatgaaatcatc 540 tg <210> 341 <211> 542 <212> DNA
<213> Ctenocephalides fells <220>
<221> misc_feature <222> (533)..(533) <223> n = unknown at position 533 <400>

attcctttttttttttttaaatattagtgtaacttttaataaaattatttactcaatatc60 ttaaaattgtttgtgatggcacatgttttataactgttttgaaaatttcgagtcacttct120 taaagttaaaatttaattaaagtcagtgtagtggtaataaataaattttggcatcaaatt180 aagtattggatatcatggcgaataataatgtgataaattttggggcaggacccgccaaac240 tcccagaagaggtgatgcttgaagtgcaagaacaattggtgcattatggtgaaacaaaaa300 tcagtgtaatggaaatgagccatcgatcaaaagactacatgaaaatcaacgatgatacac360 aaaatgcagttaaagaattattcaaataccggacaattcaaaatactatttctgcaagga420 ggtggcacaggatgtttgcagccataccactgaatattttgaagactggagttgcagatt480 atgttgttacaggttcttggtctgcaaaagctgcaaagaagcaccaaattggnaagtaac540 at 542 <210> 342 <211> 542 <212> DNA
<213> Ctenocephalides fells FC-8-C2-PCT.ST25.txt <220>
<221> misc_feature <222> (2)..(2) <223> n = unknown at position 2 <220>
<221> misc_feature <222> (33) .(33) <223> n = unknown at position 33 <220>
<221> misc_feature <222> (39) .(39) <223> n = unknown at position 39 <400>

cnttaatatactatgaatggtggagaagattgngctcangatgtgaaagagcaagaacaa60 gttaacggtggcggtgaagaaggagccggcgatgcgtctcgtgaaaatggaagcgccgaa120 gctcctggccgtgacgacgataggaaactctttgttggtggacttagttgggaaacaact180 gataaggaattgcgtgaacactttggtgcatatggagatatcgatagcattaatgttaaa240 actgaccccagtactggtcgatcacgaggatttgcattcatcgtgtattcatctcctgaa300 tccattgataaagttgttgctgtatctgaacatataattaacaacaagaaagtagatcca360 aagaaagccaaggcccgccatggaaaaatttttgttggaggtttgacgactgaagtcagc420 gatgatgatattaaaaactatttcaatcagtttgggacgatcatagaagttgagatgcca480 tttgacaagacgaagaatcaacggaaaggtttctgtttatacttttgaatctgagcaagt540 tg 542 <210>

<211>

<212>
DNA

<213>
Ctenocephalides fells <400>

aaaatactagttttattgccattaatatttgaaaaatatcattgagaaaatgtcaatatt60 attatgcgcgctattttttgcttcgacgcttagcaatgaaactttgtcgaaacaccatca120 agttaagaatcgtcgattttacatagattatgataaaaatacattcatgatggatgacaa180 accatttcgatacatagcaggttcattacattattttcgggtacatccgcaacaatggaa240 agaccgtttggaaaagctaaaagcagcaggtttaaatgccgttgatacatatgttgagtg300 gtcacttcacaattttgatgaaggaaaatattggtggggcagcaacgccgatttggagca360 atttattaaaactgcacaagaagtaggattgtatgtaattttgagaccagggccatacat420 ttgcgctgaacgtgattttggaggccaaccttattggctcttatccacccagaaaaagat480 FC-8-C2-PCT. ST25 . tact attaaaccaa ggacactgtt tcgtatatgg aagcagcacg agatggatac aagaatatta 540 as 542 <210> 344 <211> 550 <212> DNA
<213> Ctenocephalides fells <220>
<221> misc_feature <222> (538)..(538) <223> n = unknown at position 538 <400>

cttgaccgccaccgagtctgaagtggctgcactcaacaggaaggtgcaacaaattgaaga60 agatttggaaaaatctgaagaacgtgccggaagcgctaccaccaaattgcttgaagccac120 acaagctgccgatgaaaacaaccgtatgtgcaaagtattggagaacccgctcacaacaag180 atgaagagcgaatggaccaattgaccaaccaattgaaggaagcacgtctcttggctgaag240 atgctgatggtaaatctgatgaagtatcacgcaagttggccttcgttgaagatgaacttg300 aagtagctgaagaccgtgttaagggaggtgacagcaagatcatggagttggaagaagaat360 tgaaggttgtaggaaactccttgaaatctttagaagtatccgaagaaaaggccaaccaaa420 gagtagaagaattcaaacgccaattgaagactttgccgtcaaacacaaggaaccgaactc480 gtgccgagttcgccgaaaagaccgtcaagaactgaaaaggagtcgcaggctgaagacnat540 tggcataaca 550 <210> 345 <211> 550 <212> DNA
<213> Ctenocephalides fells <220>
<221> misc_feature <222> (33) . (33) <223> n = unknown at position 33 <220>
<221> misc_feature <222> (539)..(539) <223> n = unknown at position 539 <400> 345 aatcggtgta gtgcatatag gaataaaata ctngttttat tgccattaat atttgaaaaa 60 tatcattgag aaaatgtcaa tattattatg cgcgctattt tttgcttcga cgcttagcaa 120 tgaaactttg tcgaaacacc atcaagttaa gaatcgtcga ttttacatag attatgataa 180 aaatacattc atgatggatg acaaaccatt tcgatacata gcaggttcat tacattattt 240 FC-8-C2-PCT.ST25.txt tcgggtacatccgcaacaatggaaagaccgtttggaaaagctaaaagcagcaggtttaaa300 tgccgttgatacatatgttgagtggtcacttcacaattttgatgaaggaaaatattggtg360 gggcagcaacgccgatttggagcaatttattaaaactgcacaagaagtaggattgtatgt420 aattttgagaccagggccatacatttgcgctgaacgtgattttggaggccaaccttattg480 ctcttatcacacccagaaaaagatttaaaccaaggacaactgatttcgtatatggaagng540 cacgagattg 550 <210>

<211>

<212>
DNA

<213>
Ctenocephalides felis <400>

caagctgtcaaagtctggcaaatatattttaatttaataatttttgtctgttttaaataa60 aaaatgtttcggagagcgatttctagtgctactaaattgcacaaacaaactttaggtaat120 caaattgtgcaagtcaggaaccatggtggaactgccaattaccgctgtgcttcacgtcct180 tcaaaagaaattaaacttctcggtgaacttactggggctttaatgtggtactggtgcttg240 taccatatttggactgaaccggatcatattttaggagagtttccttatcctgatcctagt300 aaatggacggatgaagagcttggtatcccagccgagtaatgatggatagttaattgatag360 aaataattaaatattgaaatttaatgaagtatctggattgtatatggtactgcaatttgt420 atatagaaagtgaataagatgtgttaaacggtagtgaaatggaaaaaaaaaaaaaaaaaa480 as 482 <210>

<211>

<212>
DNA

<213>
Ctenocephalides felis <400>

gctactcattttacgtaacgttttagaagtgtgtgggtgtggtgctaattttagcaccgt60 agcgatcgtttttattaagttttttttattttgagtaatagttttaatatattttaccaa120 aatgttgcatcttaagtcaatcaccaaaagtgcactgaaacataacccctccgaagtgtc180 cacattggtcaaagctctgccaacggccatccaaacgcgctcttactccgagcaccaaat240 tcccgataggctgaaggatgtcccaacacatcctaatccaagatttttcgacatggtgga300 gtacttcttccaccgtgcctgccagatcgtcgaatctaaattggtcgaggacatgaaagg360 aagcaaaatgacagttgaagataaaactaagaaagtcaaaggtattcttatgttaatgca420 gccatgcgatcacattttggaaattgctttcccattgcgcagagattcaggaaactacga480 aatgattcagggttaccgtgctcaacacagcacacatcgacaccaccaaaggaggttccg540 FC-8-C2-PCT.ST25.txt tttcaatggt 550 <210> 348 <211> 550 < 2'12 > DNA
<213> Ctenocephalides felis <220>
<221> misc_feature <222> (523)..(523) <223> n = unknown at position 523 <400> 348 gtcacttgtagcggtttgttcgattataatatttttacgaaacaatgaaagtaatattga60 gaaacaaataatacaaatgtagttgatttcgtacattttatagcaatcttcacgatggcc120 gccggtccgatagcagaaagaaatcaagatgccactatttatgttggtggtttggacgat180 aaagtctcagaaagtcttatgtgggagctatttgttcaatctgggcctgttgtgaatgtc240 catatgccaaaagatcgagtaactcaaatgcatcaaggatatggatttgttgaatttctg300 ggagaagaagatgcagactatgcaatcaaaattatgaacatgataaaactttatggaaaa360 ccaattagggtcaataaagcttcagcacatcagaaaaatcttgatgtcggtgccaatgtg420 tttataggaaatctagacacagaagttgatgaaaaattattgatgacacatttctgtttt480 ggagtaattcttcaaacccgaagatatgagggatccaacctgnaatcaaagggtttgcat540 cataaatttg 550 <210> 349 <211> 550 <212> DNA
<213> Ctenocephalides felis <220>
<221> misc_feature <222> (547)..(547) <223> n = unknown at position 547 <400>

ttaatgtcgacggaacgcctttaacagtaaataaagaagtatttgcatcattggatgagc60 ccgcaccaggagtagtacctactcctgaacctacacctgtaccgaaacccgagcaaaaat120 gtaaaaaagtaaaatttagttgcgtgaattcgtgcagttcacccgaaatgcagtattgtc180 cggaaataggagcagatccggttaaggaatcctgtagcccagatcaagtgtgcgctgatc240 aaagtggatatctacagtgcaccactaaagaaagtacagtctgcaaagtacaaggtttca300 aatgtccgtcaccatcgagattttatccaaatataaatgattgtcaaagctattattatt360 gtgacgaaaatagtataggaacccaatattattgcccgcaaattttgcatatgatccgtt420 acgtcataattgcggcctatggctctgggcacaaaatgctatacagttacatgtctgcca480 FC-8-C2-PCT.ST25.txt gcctaaggtg cttccgtaca ttggtgataa atattgacgt cgatgtatgg ccggaagagg 540 accgtangca 550 <210>

<211>

<212>
DNA

<213>
Ctenocephalides felis <400>

cgagtttatattttatttttaataatttaatttatattattgaatgggagattattagaa 60 ttaattctataataattatttttagaatattatttgattgaatatctttaatatttataa 120 gatttgtattaataatttcttcaatagtagttaagtatagagaaagatatataatagaag 180 attttaataaaaatcgatttttattattagtattaatatttgtattttctataataataa 240 taattattagtccaaatttaattagaattttattaggttgagatggattaggtttagttt 300 cttattgtttagttatttattatcaaaatattaaatcttataatgctggtatattaactg 360 ttttaataaatcgaattggggatgtgctttattaattagaatttcttggataataaatta 420 tggtagttgaaattatttattttatataaaatatataataaataatttgaaataatttaa 480 ttatatttttgatttaatttctgcaataactaaaagagcacaaatccttttcttcttggt 540 accagctgca 550

Claims (16)

What is claimed is:

1. ~An isolated nucleic acid molecule that hybridizes to a nucleic acid sequence selected from the group consisting of a nucleic acid sequence of Table I, a nucleic acid sequence of Table II, a nucleic acid sequence complementary to a nucleic acid sequence of Table I, and a nucleic acid sequence complementary to a nucleic acid sequence of Table II, under conditions comprising (a) hybridizing in a solution comprising 1X SSC in the absence of helix destabilizing agents, at a temperature of 37°C and (b) washing in a solution comprising 1X SSC in the absence of helix destabilizing agents, at a temperature of 47.5°C.

2. ~The nucleic acid of Claim 1, wherein said nucleic acid molecule comprises a nucleic acid sequence that is at least 70% identical to a nucleic acid sequence selected from the group consisting of a nucleic acid sequence of Table I and a nucleic acid sequence of Table II.

3. ~The nucleic acid of Claim 1, wherein said nucleic acid molecule comprises a nucleic acid sequence selected from the group consisting of a nucleic acid sequence of Table I and a nucleic acid sequence of Table II.

4. ~A recombinant molecule comprising a nucleic acid molecule as set forth in Claim 1 operatively linked to a transcription control sequence.

5. ~A recombinant virus comprising a nucleic acid molecule as set forth in Claim 1.

6. ~A recombinant cell comprising a nucleic acid molecule as set forth in Claim 1.

7. ~A method to produce a protein encoded by a nucleic acid molecule that hybridizes to a nucleic acid sequence selected from the group consisting of a nucleic acid sequence complementary to a nucleic acid sequence of Table I and a nucleic acid sequence complementary to a nucleic acid sequence of Table II, under conditions comprising (a) hybridizing in a solution comprising 1X SSC in the absence of helix destabilizing agents, at a temperature of 37°C and (b) washing in a solution comprising 1X SSC in the absence of helix destabilizing agents, at a temperature of 47.5°C.

8.~The method of Claim 7, wherein said nucleic acid molecule comprises a nucleic acid sequence that is at least 70% identical to a nucleic acid sequence selected from the group consisting of a nucleic acid sequence of Table I and a nucleic acid sequence of Table II.

9. ~The method of Claim 7, wherein said nucleic acid molecule comprises a nucleic acid sequence selected from the group consisting of a nucleic acid sequence of Table I and a nucleic acid sequence of Table II.

10. An isolated protein selected from the group consisting of: an isolated protein encoded by a nucleic acid molecule that hybridizes to a nucleic acid sequence selected from the group consisting of a nucleic acid sequence complementary to a nucleic acid sequence of Table I and a nucleic acid sequence complementary to a nucleic acid sequence of Table II, under conditions comprising (a) hybridizing in a solution comprising 1X SSC in the absence of helix destabilizing agents, at a temperature of 37°C and (b) washing in a solution comprising 1X SSC in the absence of helix destabilizing agents, at a temperature of 47.5°C.

11. The protein of Claim 10, wherein said nucleic acid molecule comprises a nucleic acid sequence selected from the group consisting of a nucleic acid sequence of Table I and a nucleic acid sequence of Table II.

12. The protein of Claim 10, wherein said nucleic acid molecule comprises a nucleic acid sequence that is at least 70% identical to a nucleic acid sequence selected from the group consisting of a nucleic acid sequence of Table I and a nucleic acid sequence of Table II.

13. An isolated antibody that selectively binds to a protein as set forth in Claim 10.

14. A method to identify a compound capable of inhibiting activity of an isolated protein of Claim 10, said method comprising contacting an isolated protein of Claim 10 with a putative inhibitory compound under conditions in which, in the absence of said compound, said protein has activity; and determining if said putative inhibitory compound inhibits said activity.

15. A kit to identify a compound capable of inhibiting activity of an isolated protein of Claim 10, said test kit comprising an isolated protein of Claim 10 and a means for determining the extent of inhibition of said activity in the presence of a putative inhibitory compound.

16. A composition comprising an excipient and a compound selected from the group consisting of: (a) an isolated nucleic acid molecule of Claim 1, (b) an isolated protein of Claim 10, and (c) an isolated antibody of Claim 13.