CA2410688A1 - A system for cell-based screening - Google Patents

A system for cell-based screening Download PDF

Info

Publication number
CA2410688A1
CA2410688A1 CA002410688A CA2410688A CA2410688A1 CA 2410688 A1 CA2410688 A1 CA 2410688A1 CA 002410688 A CA002410688 A CA 002410688A CA 2410688 A CA2410688 A CA 2410688A CA 2410688 A1 CA2410688 A1 CA 2410688A1
Authority
CA
Canada
Prior art keywords
cells
locations
array
reporter molecules
microplate
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Granted
Application number
CA002410688A
Other languages
French (fr)
Other versions
CA2410688C (en
Inventor
R. Terry Dunlay
D. Lansing Taylor
Albert H. Gough
Kenneth A. Giuliano
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Cellomics Inc
Original Assignee
Individual
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Priority claimed from US08/810,983 external-priority patent/US5989835A/en
Priority claimed from US08/865,341 external-priority patent/US6103479A/en
Priority claimed from PCT/US1997/009564 external-priority patent/WO1997045730A1/en
Application filed by Individual filed Critical Individual
Priority claimed from CA002282658A external-priority patent/CA2282658C/en
Publication of CA2410688A1 publication Critical patent/CA2410688A1/en
Application granted granted Critical
Publication of CA2410688C publication Critical patent/CA2410688C/en
Anticipated expiration legal-status Critical
Expired - Lifetime legal-status Critical Current

Links

Landscapes

  • Investigating, Analyzing Materials By Fluorescence Or Luminescence (AREA)
  • Investigating Or Analysing Biological Materials (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)

Abstract

The present invention provides systems, methods, and screens for an optical system analysis of cells to rapidly determine the distribution, environment, or activity of fluorescently labeled reporter molecules in cells for the purpose of screening large numbers of compounds for those that specifically affect particular biological functions. The invention involves providing cells containing fluorescent reporter molecules in an array of locations and scanning numerous cells in each location with a high magnification fluorescence optical system, converting the optical information into digital data, and utilizing the digital data to determine the distribution, environment or activity of the fluorescently labeled reporter molecules in the cells. The array of locations may be an industry standard 96 well or 384 well microtiter plate or a microplate which is microplate having cells in a micropaterned array of locations. The invention includes apparatus and computerized method for processing, displaying and storing the data.
CA002410688A 1997-02-27 1998-02-27 A system for cell-based screening Expired - Lifetime CA2410688C (en)

Applications Claiming Priority (11)

Application Number Priority Date Filing Date Title
US08/810,983 1997-02-27
US08/810,983 US5989835A (en) 1997-02-27 1997-02-27 System for cell-based screening
US08/865,341 US6103479A (en) 1996-05-30 1997-05-29 Miniaturized cell array methods and apparatus for cell-based screening
US08/865,341 1997-05-29
WOPCT/US97/09564 1997-05-29
PCT/US1997/009564 WO1997045730A1 (en) 1996-05-30 1997-05-29 Miniaturized cell array methods and apparatus for cell-based screening
US6924697P 1997-12-11 1997-12-11
US6932997P 1997-12-11 1997-12-11
US60/069,246 1997-12-11
US60/069,329 1997-12-11
CA002282658A CA2282658C (en) 1997-02-27 1998-02-27 A system for cell-based screening

Related Parent Applications (1)

Application Number Title Priority Date Filing Date
CA002282658A Division CA2282658C (en) 1997-02-27 1998-02-27 A system for cell-based screening

Publications (2)

Publication Number Publication Date
CA2410688A1 true CA2410688A1 (en) 1998-09-03
CA2410688C CA2410688C (en) 2004-10-26

Family

ID=33425598

Family Applications (1)

Application Number Title Priority Date Filing Date
CA002410688A Expired - Lifetime CA2410688C (en) 1997-02-27 1998-02-27 A system for cell-based screening

Country Status (1)

Country Link
CA (1) CA2410688C (en)

Cited By (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP3093649A3 (en) * 1998-05-16 2017-04-26 Life Technologies Corporation An optical instrument monitoring dna polymerase chain reactions
US9823195B2 (en) 1998-05-16 2017-11-21 Life Technologies Corporation Optical instrument comprising multi-notch beam splitter
WO2023226884A1 (en) * 2022-05-24 2023-11-30 Bioarchitec Group Limited High-throughput selection and fabrication of biomaterial-encapsulated cell mass and uses thereof

Cited By (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP3093649A3 (en) * 1998-05-16 2017-04-26 Life Technologies Corporation An optical instrument monitoring dna polymerase chain reactions
US9671342B2 (en) 1998-05-16 2017-06-06 Life Technologies Corporation Instrument for monitoring polymerase chain reaction of DNA
US9823195B2 (en) 1998-05-16 2017-11-21 Life Technologies Corporation Optical instrument comprising multi-notch beam splitter
WO2023226884A1 (en) * 2022-05-24 2023-11-30 Bioarchitec Group Limited High-throughput selection and fabrication of biomaterial-encapsulated cell mass and uses thereof

Also Published As

Publication number Publication date
CA2410688C (en) 2004-10-26

Similar Documents

Publication Publication Date Title
CA2282658A1 (en) A system for cell-based screening
WO2000017643A3 (en) A system for cell-based screening
CA2348132A1 (en) A system for cell-based screening
CA2362117A1 (en) A system for cell-based screening
DE69733663T2 (en) ANALYTICAL SYSTEM AND METHOD
IL135793A (en) Process for simultaneously testing a multitude of samples of different substances for their ability to enhance or inhibit a biological process
EP1101106A4 (en) Device and method for cell motility assay
CA2381344A1 (en) Optical system analysis of cells
CA2343377A1 (en) Target analyte sensors utilizing microspheres
DE69939503D1 (en) Method and apparatus for preparing tissue samples for parallel analysis
Rockett et al. Application of DNA arrays to toxicology.
AU4564297A (en) Apparatus and method for conducting assays
CA2262739A1 (en) Low background multi-well plates for fluorescence measurements of biological and biochemical samples
WO2001011341A3 (en) A system for cell-based screening
GB9903555D0 (en) Chemical and biological assay method and apparatus
WO2000070342A3 (en) Optical system analysis of cells
EP1439384A3 (en) A system for screening biological cells
HUP0003261A2 (en) Multiple functionalities within an array element and uses thereof
CA2410688A1 (en) A system for cell-based screening
Schalkhammer Analytical biotechnology
Cortese Microarray readers: pushing the envelope: companies adopt many strategies to overcome technical challenges.(LabConsumer)
Lockhart et al. Multiplex metallica
German An Advance in Cytogenetics: Chromosome Identification. Technique and Applications in Biology and Medicine. Proceedings of a symposium, Stockholm, Sept. 1972. Torbjrn Caspersson and Lore Zech, Eds. Nobel Foundation, Stockholm, and Academic Press, New York, 1973. 256 pp., illus. $28.50. Nobel Symposium 23. Medicine and Natural Sciences.
Miller et al. Quantifying cell monolayer cultures by video imaging
Forman et al. Updated Fatigue-Crack-Growth And Fracture-Mechanics Software

Legal Events

Date Code Title Description
EEER Examination request
MKEX Expiry

Effective date: 20180227