CA2410072C - Agents de clivage et polymerases, et methode de detection connexe de l'arn - Google Patents

Agents de clivage et polymerases, et methode de detection connexe de l'arn Download PDF

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Publication number
CA2410072C
CA2410072C CA2410072A CA2410072A CA2410072C CA 2410072 C CA2410072 C CA 2410072C CA 2410072 A CA2410072 A CA 2410072A CA 2410072 A CA2410072 A CA 2410072A CA 2410072 C CA2410072 C CA 2410072C
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CA
Canada
Prior art keywords
nucleic acid
cleavage
oligonucleotide
target
probe
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Expired - Lifetime
Application number
CA2410072A
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English (en)
Other versions
CA2410072A1 (fr
Inventor
Hatim Taysir Allawi
Christian Tor Bartholomay
Luanne Chehak
Michelle L. Curtis
Peggy S. Eis
Jeff G. Hall
Hon S. Ip
Michael W. Kaiser
Robert W. Kwiatkowski, Jr.
Andrew A. Lukowiak
Victor I. Lyamichev
Wupo Ma
Marilyn C. Olson-Munoz
Sarah M. Olson
James J. Schaefer
Zbigniew Skrzypczynski
Tsetska Y. Takova
Kevin L. Vedvik
Natalie E. Lyamichev
Bruce P. Neri
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Third Wave Technologies Inc
Original Assignee
Third Wave Technologies Inc
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Priority claimed from US09/577,304 external-priority patent/US6759226B1/en
Application filed by Third Wave Technologies Inc filed Critical Third Wave Technologies Inc
Priority claimed from US09/864,426 external-priority patent/US20040018489A1/en
Priority claimed from US09/864,636 external-priority patent/US7045289B2/en
Publication of CA2410072A1 publication Critical patent/CA2410072A1/fr
Application granted granted Critical
Publication of CA2410072C publication Critical patent/CA2410072C/fr
Anticipated expiration legal-status Critical
Expired - Lifetime legal-status Critical Current

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Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6813—Hybridisation assays
    • C12Q1/6816—Hybridisation assays characterised by the detection means
    • C12Q1/6823—Release of bound markers
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/10—Transferases (2.)
    • C12N9/12—Transferases (2.) transferring phosphorus containing groups, e.g. kinases (2.7)
    • C12N9/1241—Nucleotidyltransferases (2.7.7)
    • C12N9/1252—DNA-directed DNA polymerase (2.7.7.7), i.e. DNA replicase
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/16—Hydrolases (3) acting on ester bonds (3.1)
    • C12N9/22—Ribonucleases RNAses, DNAses
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6813—Hybridisation assays
    • C12Q1/6827—Hybridisation assays for detection of mutation or polymorphism
    • C12Q1/683—Hybridisation assays for detection of mutation or polymorphism involving restriction enzymes, e.g. restriction fragment length polymorphism [RFLP]

Abstract

La présente invention se rapporte à de nouveaux agents de clivage et polymérases destinés au clivage et à la modification d'un acide nucléique. Ces agents de clivage et polymérases s'avèrent utiles, notamment, pour la détection et la caractérisation de séquences d'acides nucléiques et de variations dans des séquences d'acides nucléiques. Dans certaines réalisations, l'activité de nucléase 5' d'une variété d'enzymes est utilisée pour couper une structure à clivage dépendant de la cible, ce qui permet d'indiquer la présence de séquences d'acides nucléiques spécifiques ou de variations spécifiques de ces séquences.
CA2410072A 2000-05-24 2001-05-24 Agents de clivage et polymerases, et methode de detection connexe de l'arn Expired - Lifetime CA2410072C (fr)

Applications Claiming Priority (9)

Application Number Priority Date Filing Date Title
US09/577,304 US6759226B1 (en) 2000-05-24 2000-05-24 Enzymes for the detection of specific nucleic acid sequences
US09/577,304 2000-05-24
US09/758,282 2001-01-11
US09/758,282 US6635463B2 (en) 2000-05-24 2001-01-11 Enzymes for the detection of nucleic acid sequences
US09/864,636 2001-05-24
US09/864,426 US20040018489A1 (en) 1991-09-09 2001-05-24 Detection of RNA
US09/864,426 2001-05-24
US09/864,636 US7045289B2 (en) 1991-09-09 2001-05-24 Detection of RNA Sequences
PCT/US2001/017086 WO2001090337A2 (fr) 2000-05-24 2001-05-24 Detection d'arn

Publications (2)

Publication Number Publication Date
CA2410072A1 CA2410072A1 (fr) 2001-11-29
CA2410072C true CA2410072C (fr) 2011-07-19

Family

ID=27504919

Family Applications (1)

Application Number Title Priority Date Filing Date
CA2410072A Expired - Lifetime CA2410072C (fr) 2000-05-24 2001-05-24 Agents de clivage et polymerases, et methode de detection connexe de l'arn

Country Status (5)

Country Link
EP (1) EP1294863B1 (fr)
JP (1) JP2004521606A (fr)
AU (1) AU2001263449A1 (fr)
CA (1) CA2410072C (fr)
WO (1) WO2001090337A2 (fr)

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Also Published As

Publication number Publication date
WO2001090337A2 (fr) 2001-11-29
AU2001263449A1 (en) 2001-12-03
EP1294863A2 (fr) 2003-03-26
WO2001090337A3 (fr) 2003-01-23
JP2004521606A (ja) 2004-07-22
CA2410072A1 (fr) 2001-11-29
EP1294863B1 (fr) 2009-07-08

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