CA2405881C - Methode de separation de cellules - Google Patents

Methode de separation de cellules Download PDF

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Publication number
CA2405881C
CA2405881C CA2405881A CA2405881A CA2405881C CA 2405881 C CA2405881 C CA 2405881C CA 2405881 A CA2405881 A CA 2405881A CA 2405881 A CA2405881 A CA 2405881A CA 2405881 C CA2405881 C CA 2405881C
Authority
CA
Canada
Prior art keywords
cells
population
density
dsm
particles
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Expired - Lifetime
Application number
CA2405881A
Other languages
English (en)
Other versions
CA2405881A1 (fr
Inventor
Steven M. Woodside
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
StemCell Technologies Inc
Original Assignee
StemCell Technologies Inc
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Priority claimed from CA002358326A external-priority patent/CA2358326A1/fr
Application filed by StemCell Technologies Inc filed Critical StemCell Technologies Inc
Priority to CA2405881A priority Critical patent/CA2405881C/fr
Publication of CA2405881A1 publication Critical patent/CA2405881A1/fr
Application granted granted Critical
Publication of CA2405881C publication Critical patent/CA2405881C/fr
Anticipated expiration legal-status Critical
Expired - Lifetime legal-status Critical Current

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Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M33/00—Means for introduction, transport, positioning, extraction, harvesting, peeling or sampling of biological material in or from the apparatus
    • C12M33/10—Means for introduction, transport, positioning, extraction, harvesting, peeling or sampling of biological material in or from the apparatus by centrifugation ; Cyclones
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M47/00—Means for after-treatment of the produced biomass or of the fermentation or metabolic products, e.g. storage of biomass
    • C12M47/04—Cell isolation or sorting
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C12N5/0081—Purging biological preparations of unwanted cells
    • C12N5/0087—Purging against subsets of blood cells, e.g. purging alloreactive T cells

Abstract

La présente invention porte sur une méthode qui consiste à séparer une première population de cellules d'une seconde population de cellules d'un échantillon. Il s'agit d'effectuer une séparation à gradients de densités discontinue au moyen de particules denses pour cibler la première population de cellules, et d'un milieu de séparation de densités étant au moins environ 0,001 g/cm3 plus élevé que la densité de la seconde population de cellules.
CA2405881A 2001-10-01 2002-10-01 Methode de separation de cellules Expired - Lifetime CA2405881C (fr)

Priority Applications (1)

Application Number Priority Date Filing Date Title
CA2405881A CA2405881C (fr) 2001-10-01 2002-10-01 Methode de separation de cellules

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
CA002358326A CA2358326A1 (fr) 2001-10-01 2001-10-01 Methode de separation de cellules par centrifugation a gradient de densite discontinue
CA2,358,326 2001-10-01
CA2405881A CA2405881C (fr) 2001-10-01 2002-10-01 Methode de separation de cellules

Publications (2)

Publication Number Publication Date
CA2405881A1 CA2405881A1 (fr) 2003-04-01
CA2405881C true CA2405881C (fr) 2011-11-15

Family

ID=25682745

Family Applications (1)

Application Number Title Priority Date Filing Date
CA2405881A Expired - Lifetime CA2405881C (fr) 2001-10-01 2002-10-01 Methode de separation de cellules

Country Status (1)

Country Link
CA (1) CA2405881C (fr)

Families Citing this family (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN113308436A (zh) * 2021-06-24 2021-08-27 海南赛奥干细胞生物科技有限公司 一种人类骨髓、脐带血干细胞处理试剂盒及干细胞分离方法

Also Published As

Publication number Publication date
CA2405881A1 (fr) 2003-04-01

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