CA2402483A1 - Methodes de detection des differences entre acides nucleiques - Google Patents

Methodes de detection des differences entre acides nucleiques Download PDF

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Publication number
CA2402483A1
CA2402483A1 CA002402483A CA2402483A CA2402483A1 CA 2402483 A1 CA2402483 A1 CA 2402483A1 CA 002402483 A CA002402483 A CA 002402483A CA 2402483 A CA2402483 A CA 2402483A CA 2402483 A1 CA2402483 A1 CA 2402483A1
Authority
CA
Canada
Prior art keywords
holliday
nucleic acid
complex
difference
dna
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Abandoned
Application number
CA002402483A
Other languages
English (en)
Inventor
Qinghong Yang
Wendy Yang
Alla Lishanski
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Panomics LLC
Original Assignee
Individual
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Individual filed Critical Individual
Publication of CA2402483A1 publication Critical patent/CA2402483A1/fr
Abandoned legal-status Critical Current

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Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6813—Hybridisation assays
    • C12Q1/6827—Hybridisation assays for detection of mutation or polymorphism

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  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Microbiology (AREA)
  • Immunology (AREA)
  • Physics & Mathematics (AREA)
  • Molecular Biology (AREA)
  • Biotechnology (AREA)
  • Biophysics (AREA)
  • Analytical Chemistry (AREA)
  • Biochemistry (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)

Abstract

La présente invention concerne des méthodes permettant de détecter la présence ou l'absence d'une différence entre deux séquences d'acides nucléiques apparentées. Lesdites méthodes consistent à mettre en contact un acide nucléique cible et un acide nucléique de référence dans des conditions où ils peuvent former un complexe d'acide nucléique à quatre branches possédant une structure susceptible de migrations. Dans ces conditions de contact, si l'acide nucléique de référence et l'acide nucléique cible sont identiques, la migration entre les branches peut s'effectuer jusqu'à son terme et résulter en un échange de brins complet. Si l'acide nucléique de référence et l'acide nucléique cible sont différents, la migration entre les branches n'arrive pas à son terme, ce qui entraîne la création d'un complexe à quatre branches stable. Pour identifier la présence d'une différence entre les acides nucléiques, on utilise ledit complexe à quatre branches stable. Ledit complexe à quatre branches stable peut également être détecté à l'aide de molécules se liant spécifiquement à ces complexes, soit par électrophorèse sur gel soit par isolement spécifique du complexe à quatre branches stable.
CA002402483A 2000-03-11 2001-03-12 Methodes de detection des differences entre acides nucleiques Abandoned CA2402483A1 (fr)

Applications Claiming Priority (13)

Application Number Priority Date Filing Date Title
US18866900P 2000-03-11 2000-03-11
US60/188,669 2000-03-11
US22888500P 2000-08-29 2000-08-29
US60/228,885 2000-08-29
US23422900P 2000-09-21 2000-09-21
US60/234,229 2000-09-21
US23436300P 2000-09-22 2000-09-22
US60/234,363 2000-09-22
US24284000P 2000-10-23 2000-10-23
US24277000P 2000-10-23 2000-10-23
US60/242,840 2000-10-23
US60/242,770 2000-10-23
PCT/US2001/007858 WO2001069200A2 (fr) 2000-03-11 2001-03-12 Methodes de detection des differences entre acides nucleiques

Publications (1)

Publication Number Publication Date
CA2402483A1 true CA2402483A1 (fr) 2001-09-20

Family

ID=27558796

Family Applications (1)

Application Number Title Priority Date Filing Date
CA002402483A Abandoned CA2402483A1 (fr) 2000-03-11 2001-03-12 Methodes de detection des differences entre acides nucleiques

Country Status (6)

Country Link
EP (1) EP1266202A4 (fr)
JP (1) JP2003527119A (fr)
CN (1) CN1191374C (fr)
AU (2) AU2001245634B2 (fr)
CA (1) CA2402483A1 (fr)
WO (1) WO2001069200A2 (fr)

Families Citing this family (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2009093667A1 (fr) * 2008-01-22 2009-07-30 Hiroshima University Procédé et kit de dosage de protéine de liaison à l'acide nucléique

Family Cites Families (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
DE69531612D1 (de) * 1994-04-25 2003-10-02 Avitech Diagnostics Inc Die bestimmung von mutationen durch spaltung mit resolvase
US5824471A (en) * 1995-06-05 1998-10-20 Brigham And Women's Hospital Detection of mismatches by cleavage of nucleic acid heteroduplexes
WO1997023646A1 (fr) * 1995-12-22 1997-07-03 Behringwerke Aktiengesellschaft Detection de differences dans les acides nucleiques
WO1999036574A1 (fr) * 1998-01-20 1999-07-22 Mayo Foundation For Medical Education And Research Detection simplifiee des mutations

Also Published As

Publication number Publication date
AU2001245634B2 (en) 2005-10-06
AU4563401A (en) 2001-09-24
JP2003527119A (ja) 2003-09-16
CN1191374C (zh) 2005-03-02
EP1266202A4 (fr) 2005-11-09
WO2001069200A2 (fr) 2001-09-20
EP1266202A2 (fr) 2002-12-18
WO2001069200A3 (fr) 2002-03-14
CN1429277A (zh) 2003-07-09

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Legal Events

Date Code Title Description
FZDE Discontinued
FZDE Discontinued

Effective date: 20070312