CA2390375A1 - Appareil d'analyse et de tri de cellules servant a manipuler des cellules - Google Patents
Appareil d'analyse et de tri de cellules servant a manipuler des cellules Download PDFInfo
- Publication number
- CA2390375A1 CA2390375A1 CA002390375A CA2390375A CA2390375A1 CA 2390375 A1 CA2390375 A1 CA 2390375A1 CA 002390375 A CA002390375 A CA 002390375A CA 2390375 A CA2390375 A CA 2390375A CA 2390375 A1 CA2390375 A1 CA 2390375A1
- Authority
- CA
- Canada
- Prior art keywords
- substrate
- cell
- well
- chamber
- cells
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Abandoned
Links
- 238000004458 analytical method Methods 0.000 title abstract description 21
- 230000005684 electric field Effects 0.000 claims abstract description 18
- BASFCYQUMIYNBI-UHFFFAOYSA-N platinum Chemical compound [Pt] BASFCYQUMIYNBI-UHFFFAOYSA-N 0.000 claims description 88
- 229910052697 platinum Inorganic materials 0.000 claims description 44
- 238000000034 method Methods 0.000 claims description 35
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- XUIMIQQOPSSXEZ-UHFFFAOYSA-N Silicon Chemical compound [Si] XUIMIQQOPSSXEZ-UHFFFAOYSA-N 0.000 claims description 25
- 229910052710 silicon Inorganic materials 0.000 claims description 24
- 239000010703 silicon Substances 0.000 claims description 24
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- CSCPPACGZOOCGX-UHFFFAOYSA-N Acetone Chemical compound CC(C)=O CSCPPACGZOOCGX-UHFFFAOYSA-N 0.000 claims description 4
- 238000000151 deposition Methods 0.000 claims description 4
- 230000004913 activation Effects 0.000 claims description 2
- PCHJSUWPFVWCPO-UHFFFAOYSA-N gold Chemical compound [Au] PCHJSUWPFVWCPO-UHFFFAOYSA-N 0.000 claims description 2
- 239000010931 gold Substances 0.000 claims description 2
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- 238000012544 monitoring process Methods 0.000 abstract description 2
- 210000004027 cell Anatomy 0.000 description 176
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 description 61
- 239000002245 particle Substances 0.000 description 36
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- 241000196324 Embryophyta Species 0.000 description 2
- RTAQQCXQSZGOHL-UHFFFAOYSA-N Titanium Chemical compound [Ti] RTAQQCXQSZGOHL-UHFFFAOYSA-N 0.000 description 2
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- 244000153158 Ammi visnaga Species 0.000 description 1
- 235000010585 Ammi visnaga Nutrition 0.000 description 1
- 101100269850 Caenorhabditis elegans mask-1 gene Proteins 0.000 description 1
- OYPRJOBELJOOCE-UHFFFAOYSA-N Calcium Chemical compound [Ca] OYPRJOBELJOOCE-UHFFFAOYSA-N 0.000 description 1
- 206010013710 Drug interaction Diseases 0.000 description 1
- 102100031573 Hematopoietic progenitor cell antigen CD34 Human genes 0.000 description 1
- 101000777663 Homo sapiens Hematopoietic progenitor cell antigen CD34 Proteins 0.000 description 1
- UFHFLCQGNIYNRP-UHFFFAOYSA-N Hydrogen Chemical compound [H][H] UFHFLCQGNIYNRP-UHFFFAOYSA-N 0.000 description 1
- 108700002232 Immediate-Early Genes Proteins 0.000 description 1
- VVQNEPGJFQJSBK-UHFFFAOYSA-N Methyl methacrylate Chemical compound COC(=O)C(C)=C VVQNEPGJFQJSBK-UHFFFAOYSA-N 0.000 description 1
- 229920005372 Plexiglas® Polymers 0.000 description 1
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- 229910052782 aluminium Inorganic materials 0.000 description 1
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- 238000013459 approach Methods 0.000 description 1
- 101150036080 at gene Proteins 0.000 description 1
- QVGXLLKOCUKJST-UHFFFAOYSA-N atomic oxygen Chemical compound [O] QVGXLLKOCUKJST-UHFFFAOYSA-N 0.000 description 1
- 230000027455 binding Effects 0.000 description 1
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- 229910052791 calcium Inorganic materials 0.000 description 1
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- 230000022131 cell cycle Effects 0.000 description 1
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- 201000010099 disease Diseases 0.000 description 1
- 208000037265 diseases, disorders, signs and symptoms Diseases 0.000 description 1
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- 239000003814 drug Substances 0.000 description 1
- 238000012377 drug delivery Methods 0.000 description 1
- 238000007876 drug discovery Methods 0.000 description 1
- 235000013601 eggs Nutrition 0.000 description 1
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- 150000002500 ions Chemical class 0.000 description 1
- 239000003446 ligand Substances 0.000 description 1
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- 210000004962 mammalian cell Anatomy 0.000 description 1
- 230000000873 masking effect Effects 0.000 description 1
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- 230000004001 molecular interaction Effects 0.000 description 1
- 239000001301 oxygen Substances 0.000 description 1
- 229910052760 oxygen Inorganic materials 0.000 description 1
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- 210000005259 peripheral blood Anatomy 0.000 description 1
- 239000011886 peripheral blood Substances 0.000 description 1
- 230000003285 pharmacodynamic effect Effects 0.000 description 1
- 229910021420 polycrystalline silicon Inorganic materials 0.000 description 1
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- 238000004062 sedimentation Methods 0.000 description 1
- 230000019491 signal transduction Effects 0.000 description 1
- NTHWMYGWWRZVTN-UHFFFAOYSA-N sodium silicate Chemical compound [Na+].[Na+].[O-][Si]([O-])=O NTHWMYGWWRZVTN-UHFFFAOYSA-N 0.000 description 1
- 239000007787 solid Substances 0.000 description 1
- 230000003068 static effect Effects 0.000 description 1
- 238000005309 stochastic process Methods 0.000 description 1
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- GPRLSGONYQIRFK-MNYXATJNSA-N triton Chemical compound [3H+] GPRLSGONYQIRFK-MNYXATJNSA-N 0.000 description 1
- 238000009834 vaporization Methods 0.000 description 1
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- 239000002699 waste material Substances 0.000 description 1
- 210000005253 yeast cell Anatomy 0.000 description 1
Classifications
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N15/00—Investigating characteristics of particles; Investigating permeability, pore-volume or surface-area of porous materials
- G01N15/10—Investigating individual particles
- G01N15/14—Optical investigation techniques, e.g. flow cytometry
- G01N15/1484—Optical investigation techniques, e.g. flow cytometry microstructural devices
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N15/00—Investigating characteristics of particles; Investigating permeability, pore-volume or surface-area of porous materials
- G01N15/10—Investigating individual particles
- G01N15/14—Optical investigation techniques, e.g. flow cytometry
- G01N15/1456—Optical investigation techniques, e.g. flow cytometry without spatial resolution of the texture or inner structure of the particle, e.g. processing of pulse signals
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N15/00—Investigating characteristics of particles; Investigating permeability, pore-volume or surface-area of porous materials
- G01N15/10—Investigating individual particles
- G01N15/14—Optical investigation techniques, e.g. flow cytometry
- G01N15/1468—Optical investigation techniques, e.g. flow cytometry with spatial resolution of the texture or inner structure of the particle
- G01N15/147—Optical investigation techniques, e.g. flow cytometry with spatial resolution of the texture or inner structure of the particle the analysis being performed on a sample stream
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N30/00—Investigating or analysing materials by separation into components using adsorption, absorption or similar phenomena or using ion-exchange, e.g. chromatography or field flow fractionation
- G01N30/0005—Field flow fractionation
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01J—CHEMICAL OR PHYSICAL PROCESSES, e.g. CATALYSIS OR COLLOID CHEMISTRY; THEIR RELEVANT APPARATUS
- B01J2219/00—Chemical, physical or physico-chemical processes in general; Their relevant apparatus
- B01J2219/00274—Sequential or parallel reactions; Apparatus and devices for combinatorial chemistry or for making arrays; Chemical library technology
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N15/00—Investigating characteristics of particles; Investigating permeability, pore-volume or surface-area of porous materials
- G01N15/10—Investigating individual particles
- G01N15/14—Optical investigation techniques, e.g. flow cytometry
- G01N15/149—Optical investigation techniques, e.g. flow cytometry specially adapted for sorting particles, e.g. by their size or optical properties
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N15/00—Investigating characteristics of particles; Investigating permeability, pore-volume or surface-area of porous materials
- G01N15/10—Investigating individual particles
- G01N15/14—Optical investigation techniques, e.g. flow cytometry
- G01N15/1404—Handling flow, e.g. hydrodynamic focusing
- G01N2015/1415—Control of particle position
Landscapes
- Chemical & Material Sciences (AREA)
- Biochemistry (AREA)
- Physics & Mathematics (AREA)
- Health & Medical Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Analytical Chemistry (AREA)
- General Health & Medical Sciences (AREA)
- General Physics & Mathematics (AREA)
- Immunology (AREA)
- Pathology (AREA)
- Dispersion Chemistry (AREA)
- Apparatus Associated With Microorganisms And Enzymes (AREA)
- Investigating Or Analysing Biological Materials (AREA)
- Sampling And Sample Adjustment (AREA)
Abstract
L'invention concerne un appareil d'analyse et de tri de cellules pouvant surveiller, au fil du temps, le comportement de chaque cellule au sein d'une grande population de cellules. L'appareil d'analyse et de tri de cellules contient des emplacements de cellules portant une adresse distincte. Chaque emplacement peut piéger et retenir une seule cellule, et libérer sélectivement ladite cellule dudit emplacement particulier. Dans un aspect de l'invention, les cellules sont piégées et retenues dans des cupules; elles sont libérées par des bulles de vapeur utilisées comme moyens d'activation. Dans un autre aspect de l'invention, les cellules sont piégées, retenues et libérées au moyen de pièges à champs électriques.
Applications Claiming Priority (3)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
US16464399P | 1999-11-10 | 1999-11-10 | |
US60/164,643 | 1999-11-10 | ||
PCT/US2000/030815 WO2001035071A2 (fr) | 1999-11-10 | 2000-11-10 | Appareil d'analyse et de tri de cellules servant a manipuler des cellules |
Publications (1)
Publication Number | Publication Date |
---|---|
CA2390375A1 true CA2390375A1 (fr) | 2001-05-17 |
Family
ID=22595424
Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
CA002390375A Abandoned CA2390375A1 (fr) | 1999-11-10 | 2000-11-10 | Appareil d'analyse et de tri de cellules servant a manipuler des cellules |
Country Status (5)
Country | Link |
---|---|
EP (1) | EP1236032A2 (fr) |
JP (1) | JP2003514236A (fr) |
AU (1) | AU1592501A (fr) |
CA (1) | CA2390375A1 (fr) |
WO (1) | WO2001035071A2 (fr) |
Families Citing this family (36)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
US20020064808A1 (en) * | 2000-11-29 | 2002-05-30 | Mutz Mitchell W. | Focused acoustic energy for ejecting cells from a fluid |
US6893836B2 (en) | 2000-11-29 | 2005-05-17 | Picoliter Inc. | Spatially directed ejection of cells from a carrier fluid |
EP2359689B1 (fr) | 2002-09-27 | 2015-08-26 | The General Hospital Corporation | Dispositif microfluidique pour la séparation de cellules et usage du dispositif |
IL154677A0 (en) | 2003-02-27 | 2003-09-17 | Univ Bar Ilan | A method and apparatus for manipulating an individual cell |
US9200245B2 (en) | 2003-06-26 | 2015-12-01 | Seng Enterprises Ltd. | Multiwell plate |
US7888110B2 (en) | 2003-06-26 | 2011-02-15 | Seng Enterprises Ltd. | Pico liter well holding device and method of making the same |
EP1711166A1 (fr) | 2004-02-03 | 2006-10-18 | Chemagis Ltd. | Formes amorphes stables de montelukast sodique |
ATE444045T1 (de) | 2004-03-08 | 2009-10-15 | Allergan Medical S A | Verschlusssystem für röhrenförmige organe |
US7403647B2 (en) | 2004-09-13 | 2008-07-22 | Seng Enterprises Ltd. | Method for identifying an image of a well in an image of a well-bearing component |
DE602005023534D1 (de) * | 2005-01-25 | 2010-10-21 | Seng Entpr Ltd | Mikrofluidische vorrichtung zur untersuchung zellen |
US20070196820A1 (en) | 2005-04-05 | 2007-08-23 | Ravi Kapur | Devices and methods for enrichment and alteration of cells and other particles |
GB0508983D0 (en) | 2005-05-03 | 2005-06-08 | Oxford Gene Tech Ip Ltd | Cell analyser |
US8921102B2 (en) | 2005-07-29 | 2014-12-30 | Gpb Scientific, Llc | Devices and methods for enrichment and alteration of circulating tumor cells and other particles |
EP2589668A1 (fr) | 2006-06-14 | 2013-05-08 | Verinata Health, Inc | Analyse de cellules rares utilisant la division d'échantillons et les marqueurs d'ADN |
US20080070792A1 (en) | 2006-06-14 | 2008-03-20 | Roland Stoughton | Use of highly parallel snp genotyping for fetal diagnosis |
US20080050739A1 (en) | 2006-06-14 | 2008-02-28 | Roland Stoughton | Diagnosis of fetal abnormalities using polymorphisms including short tandem repeats |
US8137912B2 (en) | 2006-06-14 | 2012-03-20 | The General Hospital Corporation | Methods for the diagnosis of fetal abnormalities |
CA2655272C (fr) | 2006-06-14 | 2017-04-18 | Living Microsystems, Inc. | Analyse de cellules rares avec recours au fractionnement d'echantillons et a des marqueurs d'adn |
WO2009081409A2 (fr) | 2007-12-26 | 2009-07-02 | Seng Enterprises Ltd. | Dispositif pour l'étude de cellules vivantes |
US9145540B1 (en) | 2007-11-15 | 2015-09-29 | Seng Enterprises Ltd. | Device for the study of living cells |
CA3069082C (fr) | 2008-09-20 | 2022-03-22 | The Board Of Trustees Of The Leland Stanford Junior University | Diagnostic non effractif d'aneuploidie foetale par sequencage |
JP2011000079A (ja) * | 2009-06-19 | 2011-01-06 | Univ Of Tokyo | 粒子を操作する方法及びマイクロ流体装置 |
US9364831B2 (en) | 2009-08-08 | 2016-06-14 | The Regents Of The University Of California | Pulsed laser triggered high speed microfluidic switch and applications in fluorescent activated cell sorting |
US9176504B2 (en) | 2011-02-11 | 2015-11-03 | The Regents Of The University Of California | High-speed on demand droplet generation and single cell encapsulation driven by induced cavitation |
CN110186835B (zh) | 2013-03-15 | 2022-05-31 | Gpb科学有限公司 | 颗粒的片上微流体处理 |
JP6461905B2 (ja) | 2013-03-15 | 2019-01-30 | ザ リージェンツ オブ ザ ユニバーシティ オブ カリフォルニア | 高速オンデマンド型マイクロ流体液滴生成及び操作 |
US20150064153A1 (en) | 2013-03-15 | 2015-03-05 | The Trustees Of Princeton University | High efficiency microfluidic purification of stem cells to improve transplants |
CN105247042B (zh) | 2013-03-15 | 2021-06-11 | 普林斯顿大学理事会 | 用于高通量纯化的方法和设备 |
JP6334274B2 (ja) * | 2014-06-03 | 2018-05-30 | 積水化学工業株式会社 | 懸濁液の移送方法 |
US10124338B2 (en) * | 2014-11-19 | 2018-11-13 | Imec Vzw | Microbubble generator device, systems and method to fabricate |
JP6964590B2 (ja) * | 2015-12-30 | 2021-11-10 | バークレー ライツ,インコーポレイテッド | 光学的に駆動される対流及び変位のマイクロ流体デバイス、そのキット及び方法 |
WO2019190488A1 (fr) | 2018-03-27 | 2019-10-03 | Hewlett-Packard Development Company, L.P. | Séparation de particules |
JP2020027068A (ja) * | 2018-08-15 | 2020-02-20 | ソニー株式会社 | 粒子取得方法、粒子捕捉用チャンバ、及び粒子分析システム |
CN116174068A (zh) | 2019-04-30 | 2023-05-30 | 伯克利之光生命科技公司 | 用于包封和测定细胞的方法 |
US11492585B2 (en) | 2019-05-31 | 2022-11-08 | Canon Medical Systems Corporation | Cell identification system and cell identification method |
WO2024151030A1 (fr) * | 2023-01-09 | 2024-07-18 | 한국화학연구원 | Plaque de mesure pour dispositif de mesure d'impédance de sphéroïdes de cellules |
Family Cites Families (4)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
US5310674A (en) * | 1982-05-10 | 1994-05-10 | Bar-Ilan University | Apertured cell carrier |
JP2662215B2 (ja) * | 1986-11-19 | 1997-10-08 | 株式会社日立製作所 | 細胞保持装置 |
DE19712309A1 (de) * | 1996-11-16 | 1998-05-20 | Nmi Univ Tuebingen | Mikroelementenanordnung, Verfahren zum Kontaktieren von in einer flüssigen Umgebung befindlichen Zellen und Verfahren zum Herstellen einer Mikroelementenanordnung |
AU746580B2 (en) * | 1997-12-17 | 2002-05-02 | Ecole Polytechnique Federale De Lausanne | Positioning and electrophysiological characterization of individual cells and reconstituted membrane systems on microstructured carriers |
-
2000
- 2000-11-10 WO PCT/US2000/030815 patent/WO2001035071A2/fr active Application Filing
- 2000-11-10 CA CA002390375A patent/CA2390375A1/fr not_active Abandoned
- 2000-11-10 JP JP2001536950A patent/JP2003514236A/ja not_active Withdrawn
- 2000-11-10 EP EP00978464A patent/EP1236032A2/fr not_active Withdrawn
- 2000-11-10 AU AU15925/01A patent/AU1592501A/en not_active Abandoned
Also Published As
Publication number | Publication date |
---|---|
AU1592501A (en) | 2001-06-06 |
WO2001035071A2 (fr) | 2001-05-17 |
WO2001035071A3 (fr) | 2002-02-21 |
JP2003514236A (ja) | 2003-04-15 |
EP1236032A2 (fr) | 2002-09-04 |
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