CA2387605A1 - High yield protein expression system and methods - Google Patents

High yield protein expression system and methods Download PDF

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Publication number
CA2387605A1
CA2387605A1 CA002387605A CA2387605A CA2387605A1 CA 2387605 A1 CA2387605 A1 CA 2387605A1 CA 002387605 A CA002387605 A CA 002387605A CA 2387605 A CA2387605 A CA 2387605A CA 2387605 A1 CA2387605 A1 CA 2387605A1
Authority
CA
Canada
Prior art keywords
cell
pyruvate carboxylase
protein
metabolically engineered
peptide
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Abandoned
Application number
CA002387605A
Other languages
English (en)
French (fr)
Inventor
Mark A. Eiteman
Elliot Altman
Ravi R. Gokarn
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
University of Georgia Research Foundation Inc UGARF
Original Assignee
Individual
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Individual filed Critical Individual
Publication of CA2387605A1 publication Critical patent/CA2387605A1/en
Abandoned legal-status Critical Current

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Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/93—Ligases (6)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P13/00—Preparation of nitrogen-containing organic compounds
    • C12P13/04—Alpha- or beta- amino acids
    • C12P13/08—Lysine; Diaminopimelic acid; Threonine; Valine
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P21/00—Preparation of peptides or proteins
    • C12P21/02—Preparation of peptides or proteins having a known sequence of two or more amino acids, e.g. glutathione

Landscapes

  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Zoology (AREA)
  • Engineering & Computer Science (AREA)
  • Wood Science & Technology (AREA)
  • Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Microbiology (AREA)
  • Biochemistry (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Biotechnology (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • General Chemical & Material Sciences (AREA)
  • Molecular Biology (AREA)
  • Medicinal Chemistry (AREA)
  • Biomedical Technology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Enzymes And Modification Thereof (AREA)
CA002387605A 1999-10-13 2000-10-13 High yield protein expression system and methods Abandoned CA2387605A1 (en)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
US15946799P 1999-10-13 1999-10-13
US60/159,467 1999-10-13
PCT/US2000/028578 WO2001027258A2 (en) 1999-10-13 2000-10-13 High yield protein expression system and methods

Publications (1)

Publication Number Publication Date
CA2387605A1 true CA2387605A1 (en) 2001-04-19

Family

ID=22572721

Family Applications (1)

Application Number Title Priority Date Filing Date
CA002387605A Abandoned CA2387605A1 (en) 1999-10-13 2000-10-13 High yield protein expression system and methods

Country Status (9)

Country Link
EP (1) EP1235903A2 (pt)
JP (1) JP2003511067A (pt)
AR (1) AR026892A1 (pt)
AU (1) AU1206801A (pt)
BR (1) BR0014758A (pt)
CA (1) CA2387605A1 (pt)
HK (1) HK1052198A1 (pt)
MX (1) MXPA02003634A (pt)
WO (1) WO2001027258A2 (pt)

Families Citing this family (18)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
KR100397423B1 (ko) 2001-02-13 2003-09-13 씨제이 주식회사 Ｌ-쓰레오닌의 제조방법
US7368266B2 (en) 2001-02-13 2008-05-06 Cj Corporation Method for L-threonine production
US8119365B2 (en) 2002-01-23 2012-02-21 Wisconsin Alumni Research Foundation Insertion sequence-free bacteria
US8765408B2 (en) 2002-01-23 2014-07-01 Wisconsin Alumni Research Foundation Prophage element-free bacteria
US8039243B2 (en) 2002-01-23 2011-10-18 Wisconsin Alumni Research Foundation Insertion sequence-free bacteria
US6989265B2 (en) 2002-01-23 2006-01-24 Wisconsin Alumni Research Foundation Bacteria with reduced genome
DE10219714A1 (de) * 2002-05-02 2003-11-27 Holland Sweetener Co Verfahren zur mikrobielien Herstellung von aromatischen Aminosäuren und anderen Metaboliten des aromatischen Aminosäurebiosyntheseweges
US7303906B2 (en) 2002-09-06 2007-12-04 Wisconsin Alumni Research Foundation Competent bacteria
US9453251B2 (en) 2002-10-08 2016-09-27 Pfenex Inc. Expression of mammalian proteins in Pseudomonas fluorescens
PL1709170T3 (pl) 2004-01-16 2018-06-29 Pfenex Inc. Ekspresja białek ssaczych w Pseudomonas fluorescens
WO2005087940A1 (en) * 2004-03-11 2005-09-22 Wisconsin Alumni Research Foundation Genetically altered microorganisms with modified metabolism
EP1584680A1 (en) * 2004-04-08 2005-10-12 Boehringer Ingelheim Austria GmbH Fed-batch fermentation process for the production of plasmid DNA
JP4720114B2 (ja) * 2004-05-20 2011-07-13 三菱化学株式会社 オキザロ酢酸またはオキザロ酢酸誘導体の製造方法
WO2006014899A2 (en) 2004-07-26 2006-02-09 Dow Global Technologies Inc. Process for improved protein expression by strain engineering
US9580719B2 (en) 2007-04-27 2017-02-28 Pfenex, Inc. Method for rapidly screening microbial hosts to identify certain strains with improved yield and/or quality in the expression of heterologous proteins
US9394571B2 (en) 2007-04-27 2016-07-19 Pfenex Inc. Method for rapidly screening microbial hosts to identify certain strains with improved yield and/or quality in the expression of heterologous proteins
EP2692911B1 (en) 2011-03-31 2017-02-22 Kunimine Industries Co., Ltd. Use of agent for searching for protein crystallization conditions, and method for searching for protein crystallization conditions
JP2015156844A (ja) * 2014-02-25 2015-09-03 花王株式会社 枯草菌変異株及びそれを用いたジピコリン酸の製造方法

Family Cites Families (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO1995006114A1 (fr) * 1993-08-24 1995-03-02 Ajinomoto Co., Inc. Allele de phosphenolpyruvate carboxylase, gene de cet allele et procede de production de l'acide amine
DE69942022D1 (de) * 1998-04-13 2010-04-01 Univ Georgia Pyruvate carboxylase überexpression zur verstärkten produktion von oxalacetat abgeleiteten verbindungen in mikrobiellen zellen
DE19904794A1 (de) * 1999-02-05 2000-08-10 Biotechnolog Forschung Gmbh Verfahren zur Verbesserung des Primärstoffwechsels von Säugerzelllinien

Also Published As

Publication number Publication date
MXPA02003634A (es) 2002-10-23
WO2001027258A2 (en) 2001-04-19
AR026892A1 (es) 2003-03-05
BR0014758A (pt) 2002-07-02
HK1052198A1 (zh) 2003-09-05
EP1235903A2 (en) 2002-09-04
AU1206801A (en) 2001-04-23
WO2001027258A3 (en) 2001-12-27
JP2003511067A (ja) 2003-03-25

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