CA2386722A1 - Materiaux et procedes pour la production simplifiee d'aav - Google Patents

Materiaux et procedes pour la production simplifiee d'aav Download PDF

Info

Publication number
CA2386722A1
CA2386722A1 CA002386722A CA2386722A CA2386722A1 CA 2386722 A1 CA2386722 A1 CA 2386722A1 CA 002386722 A CA002386722 A CA 002386722A CA 2386722 A CA2386722 A CA 2386722A CA 2386722 A1 CA2386722 A1 CA 2386722A1
Authority
CA
Canada
Prior art keywords
aav
host cell
replication
adenovirus
construct
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Abandoned
Application number
CA002386722A
Other languages
English (en)
Inventor
Varavani Dwarki
Martha Ladner
Jaime Escobedo
Shang-Zhen Zhou
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Novartis Vaccines and Diagnostics Inc
Original Assignee
Individual
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Individual filed Critical Individual
Publication of CA2386722A1 publication Critical patent/CA2386722A1/fr
Abandoned legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/85—Vectors or expression systems specially adapted for eukaryotic hosts for animal cells
    • C12N15/86—Viral vectors
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2750/00—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA ssDNA viruses
    • C12N2750/00011—Details
    • C12N2750/14011—Parvoviridae
    • C12N2750/14111—Dependovirus, e.g. adenoassociated viruses
    • C12N2750/14141—Use of virus, viral particle or viral elements as a vector
    • C12N2750/14143—Use of virus, viral particle or viral elements as a vector viral genome or elements thereof as genetic vector

Landscapes

  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Engineering & Computer Science (AREA)
  • Zoology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biomedical Technology (AREA)
  • Organic Chemistry (AREA)
  • Biotechnology (AREA)
  • General Engineering & Computer Science (AREA)
  • Chemical & Material Sciences (AREA)
  • Wood Science & Technology (AREA)
  • Microbiology (AREA)
  • Physics & Mathematics (AREA)
  • Plant Pathology (AREA)
  • Virology (AREA)
  • Molecular Biology (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Biophysics (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Medicines Containing Material From Animals Or Micro-Organisms (AREA)
  • Medicines That Contain Protein Lipid Enzymes And Other Medicines (AREA)

Abstract

L'invention concerne un procédé relatif à la production de virions AAV de recombinaison purifiés à déficience de réplication, qui consiste à introduire dans une cellule hôte appropriée un vecteur AAV, un produit de synthèse assistant AAV et un produit de synthèse adénoplasmide accessoire. Le plasmide adénoplasmide accessoire est composé d'ADN de plasmide d'adénovirus impossible à empaqueter dans des particules adénovirales car ne possédant pas de séquence(s) de signaux d'empaquetage ou parce que ces particules contiennent des séquences additionnelles leur conférant une taille trop importante pour l'empaquetage. On met en culture la cellule hôte pour produire des virions rAAV bruts, et la cellule est ensuite soumis à une lyse. Le lysat de cellule résultant est appliqué à une colonne de chromatographie renfermant de la cellulose sulfonée ou soumis à une centrifugation à gradient d'équilibre au chlorure de césium, et on peut ainsi récupérer les virions rAAV purifiés.
CA002386722A 1999-09-30 1999-09-30 Materiaux et procedes pour la production simplifiee d'aav Abandoned CA2386722A1 (fr)

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
PCT/US1999/022789 WO2001025464A1 (fr) 1999-09-30 1999-09-30 Materiaux et procedes pour la production simplifiee d'aav

Publications (1)

Publication Number Publication Date
CA2386722A1 true CA2386722A1 (fr) 2001-04-12

Family

ID=22273719

Family Applications (1)

Application Number Title Priority Date Filing Date
CA002386722A Abandoned CA2386722A1 (fr) 1999-09-30 1999-09-30 Materiaux et procedes pour la production simplifiee d'aav

Country Status (5)

Country Link
EP (1) EP1220938A1 (fr)
JP (1) JP2003511038A (fr)
AU (1) AU1441400A (fr)
CA (1) CA2386722A1 (fr)
WO (1) WO2001025464A1 (fr)

Families Citing this family (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN103476399A (zh) * 2011-04-18 2013-12-25 独立行政法人国立精神·神经医疗研究中心 药剂递送粒子及其制造方法

Family Cites Families (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO1994012649A2 (fr) * 1992-12-03 1994-06-09 Genzyme Corporation Therapie genique de la fibrose kystique
WO1999006562A1 (fr) * 1997-07-31 1999-02-11 Chiron Corporation Procede permettant de readministrer un vecteur viral adeno-associe via l'immunodepression de l'hote

Also Published As

Publication number Publication date
AU1441400A (en) 2001-05-10
JP2003511038A (ja) 2003-03-25
EP1220938A1 (fr) 2002-07-10
WO2001025464A1 (fr) 2001-04-12

Similar Documents

Publication Publication Date Title
US6221646B1 (en) Materials and methods for simplified AAV production
US7037713B2 (en) High-efficiency wild-type-free AAV helper functions
US6897063B2 (en) Accessory functions for use in recombinant AAV virion production
US7238526B2 (en) Methods and cell line useful for production of recombinant adeno-associated viruses
US7094604B2 (en) Production of pseudotyped recombinant AAV virions
AU706647B2 (en) Process for preparing recombinant adeno-associated viruses (AAVs), and uses
US20040087026A1 (en) Host cells for packing a recombinant adeno-associated virus (raav), method for the production and use thereof
US5945335A (en) Adenovirus helper-free system for producing recombinant AAV virions lacking oncogenic sequences
WO2000011149A1 (fr) Procedes de production de virus associes aux adenovirus recombinants a titre eleve
US6303371B1 (en) Method of purified rAAV vector production in non-human cell line transfected with cocksackie and adenovirus receptor
US20020177222A1 (en) Replication competent AAV helper functions
US7148341B2 (en) AAV DNA comprising helper virus sequences
US20020045250A1 (en) Methods for purified AAV vector production
CA2386722A1 (fr) Materiaux et procedes pour la production simplifiee d'aav
US6541012B2 (en) System for the production of AAV vectors
Weger High-Level rAAV Vector Production by rAdV-Mediated Amplification of Small Amounts of Input Vector. Viruses 2023, 15, 64

Legal Events

Date Code Title Description
FZDE Discontinued