CA2386722A1 - Materiaux et procedes pour la production simplifiee d'aav - Google Patents
Materiaux et procedes pour la production simplifiee d'aav Download PDFInfo
- Publication number
- CA2386722A1 CA2386722A1 CA002386722A CA2386722A CA2386722A1 CA 2386722 A1 CA2386722 A1 CA 2386722A1 CA 002386722 A CA002386722 A CA 002386722A CA 2386722 A CA2386722 A CA 2386722A CA 2386722 A1 CA2386722 A1 CA 2386722A1
- Authority
- CA
- Canada
- Prior art keywords
- aav
- host cell
- replication
- adenovirus
- construct
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Abandoned
Links
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
- C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
- C12N15/85—Vectors or expression systems specially adapted for eukaryotic hosts for animal cells
- C12N15/86—Viral vectors
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N2750/00—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA ssDNA viruses
- C12N2750/00011—Details
- C12N2750/14011—Parvoviridae
- C12N2750/14111—Dependovirus, e.g. adenoassociated viruses
- C12N2750/14141—Use of virus, viral particle or viral elements as a vector
- C12N2750/14143—Use of virus, viral particle or viral elements as a vector viral genome or elements thereof as genetic vector
Landscapes
- Life Sciences & Earth Sciences (AREA)
- Health & Medical Sciences (AREA)
- Genetics & Genomics (AREA)
- Engineering & Computer Science (AREA)
- Zoology (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Biomedical Technology (AREA)
- Organic Chemistry (AREA)
- Biotechnology (AREA)
- General Engineering & Computer Science (AREA)
- Chemical & Material Sciences (AREA)
- Wood Science & Technology (AREA)
- Microbiology (AREA)
- Physics & Mathematics (AREA)
- Plant Pathology (AREA)
- Virology (AREA)
- Molecular Biology (AREA)
- Biochemistry (AREA)
- General Health & Medical Sciences (AREA)
- Biophysics (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
- Medicines Containing Material From Animals Or Micro-Organisms (AREA)
- Medicines That Contain Protein Lipid Enzymes And Other Medicines (AREA)
Abstract
L'invention concerne un procédé relatif à la production de virions AAV de recombinaison purifiés à déficience de réplication, qui consiste à introduire dans une cellule hôte appropriée un vecteur AAV, un produit de synthèse assistant AAV et un produit de synthèse adénoplasmide accessoire. Le plasmide adénoplasmide accessoire est composé d'ADN de plasmide d'adénovirus impossible à empaqueter dans des particules adénovirales car ne possédant pas de séquence(s) de signaux d'empaquetage ou parce que ces particules contiennent des séquences additionnelles leur conférant une taille trop importante pour l'empaquetage. On met en culture la cellule hôte pour produire des virions rAAV bruts, et la cellule est ensuite soumis à une lyse. Le lysat de cellule résultant est appliqué à une colonne de chromatographie renfermant de la cellulose sulfonée ou soumis à une centrifugation à gradient d'équilibre au chlorure de césium, et on peut ainsi récupérer les virions rAAV purifiés.
Applications Claiming Priority (1)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
PCT/US1999/022789 WO2001025464A1 (fr) | 1999-09-30 | 1999-09-30 | Materiaux et procedes pour la production simplifiee d'aav |
Publications (1)
Publication Number | Publication Date |
---|---|
CA2386722A1 true CA2386722A1 (fr) | 2001-04-12 |
Family
ID=22273719
Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
CA002386722A Abandoned CA2386722A1 (fr) | 1999-09-30 | 1999-09-30 | Materiaux et procedes pour la production simplifiee d'aav |
Country Status (5)
Country | Link |
---|---|
EP (1) | EP1220938A1 (fr) |
JP (1) | JP2003511038A (fr) |
AU (1) | AU1441400A (fr) |
CA (1) | CA2386722A1 (fr) |
WO (1) | WO2001025464A1 (fr) |
Families Citing this family (1)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
CN103476399A (zh) * | 2011-04-18 | 2013-12-25 | 独立行政法人国立精神·神经医疗研究中心 | 药剂递送粒子及其制造方法 |
Family Cites Families (2)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
WO1994012649A2 (fr) * | 1992-12-03 | 1994-06-09 | Genzyme Corporation | Therapie genique de la fibrose kystique |
WO1999006562A1 (fr) * | 1997-07-31 | 1999-02-11 | Chiron Corporation | Procede permettant de readministrer un vecteur viral adeno-associe via l'immunodepression de l'hote |
-
1999
- 1999-09-30 EP EP99974094A patent/EP1220938A1/fr not_active Withdrawn
- 1999-09-30 WO PCT/US1999/022789 patent/WO2001025464A1/fr not_active Application Discontinuation
- 1999-09-30 AU AU14414/00A patent/AU1441400A/en not_active Abandoned
- 1999-09-30 CA CA002386722A patent/CA2386722A1/fr not_active Abandoned
- 1999-09-30 JP JP2001528615A patent/JP2003511038A/ja not_active Withdrawn
Also Published As
Publication number | Publication date |
---|---|
AU1441400A (en) | 2001-05-10 |
JP2003511038A (ja) | 2003-03-25 |
EP1220938A1 (fr) | 2002-07-10 |
WO2001025464A1 (fr) | 2001-04-12 |
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Legal Events
Date | Code | Title | Description |
---|---|---|---|
FZDE | Discontinued |