CA2382121A1 - Procedes pour l'immobilisation de ligands sur des supports solides et dispositif et ses procedes d'utilisation - Google Patents

Procedes pour l'immobilisation de ligands sur des supports solides et dispositif et ses procedes d'utilisation Download PDF

Info

Publication number
CA2382121A1
CA2382121A1 CA002382121A CA2382121A CA2382121A1 CA 2382121 A1 CA2382121 A1 CA 2382121A1 CA 002382121 A CA002382121 A CA 002382121A CA 2382121 A CA2382121 A CA 2382121A CA 2382121 A1 CA2382121 A1 CA 2382121A1
Authority
CA
Canada
Prior art keywords
solid support
group
immobilized
thiol groups
nucleic acids
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Abandoned
Application number
CA002382121A
Other languages
English (en)
Inventor
Ezra S. Abrams
Tianhong Zhang
Brian C. Patterson
Slawomir Mielewczyk
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Matrix Technologies LLC
Original Assignee
Individual
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Individual filed Critical Individual
Publication of CA2382121A1 publication Critical patent/CA2382121A1/fr
Abandoned legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07H—SUGARS; DERIVATIVES THEREOF; NUCLEOSIDES; NUCLEOTIDES; NUCLEIC ACIDS
    • C07H21/00—Compounds containing two or more mononucleotide units having separate phosphate or polyphosphate groups linked by saccharide radicals of nucleoside groups, e.g. nucleic acids
    • C07H21/04—Compounds containing two or more mononucleotide units having separate phosphate or polyphosphate groups linked by saccharide radicals of nucleoside groups, e.g. nucleic acids with deoxyribosyl as saccharide radical
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6813—Hybridisation assays
    • C12Q1/6834—Enzymatic or biochemical coupling of nucleic acids to a solid phase
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6813—Hybridisation assays
    • C12Q1/6834—Enzymatic or biochemical coupling of nucleic acids to a solid phase
    • C12Q1/6837—Enzymatic or biochemical coupling of nucleic acids to a solid phase using probe arrays or probe chips
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
    • G01N33/543—Immunoassay; Biospecific binding assay; Materials therefor with an insoluble carrier for immobilising immunochemicals
    • G01N33/54353—Immunoassay; Biospecific binding assay; Materials therefor with an insoluble carrier for immobilising immunochemicals with ligand attached to the carrier via a chemical coupling agent

Landscapes

  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Organic Chemistry (AREA)
  • Engineering & Computer Science (AREA)
  • Immunology (AREA)
  • Molecular Biology (AREA)
  • Biochemistry (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Biotechnology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • General Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Microbiology (AREA)
  • Analytical Chemistry (AREA)
  • Physics & Mathematics (AREA)
  • Hematology (AREA)
  • Urology & Nephrology (AREA)
  • General Engineering & Computer Science (AREA)
  • Biomedical Technology (AREA)
  • Biophysics (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Food Science & Technology (AREA)
  • Cell Biology (AREA)
  • Medicinal Chemistry (AREA)
  • General Physics & Mathematics (AREA)
  • Pathology (AREA)
  • Apparatus Associated With Microorganisms And Enzymes (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Saccharide Compounds (AREA)

Abstract

La présente invention concerne un procédé permettant d'immobiliser un ligand, par exemple un acide nucléique, sur un support solide. Le procédé consiste à se munir d'un support solide contenant un groupe thiol latent immobilisé, à activer le groupe thiol, à mettre en contact le groupe thiol activé avec un acide nucléique comprenant un groupe acrylamide, et à former la liaison covalente entre les deux groupes, immobilisant ainsi l'acide nucléique sur le support solide. La présente invention concerne également des nécessaires contenant les supports solides et un procédé d'utilisation de tels supports.
CA002382121A 1999-08-27 2000-08-28 Procedes pour l'immobilisation de ligands sur des supports solides et dispositif et ses procedes d'utilisation Abandoned CA2382121A1 (fr)

Applications Claiming Priority (5)

Application Number Priority Date Filing Date Title
US15126799P 1999-08-27 1999-08-27
US60/151,267 1999-08-27
US17784400P 2000-01-25 2000-01-25
US60/177,844 2000-01-25
PCT/US2000/023627 WO2001016372A1 (fr) 1999-08-27 2000-08-28 Procedes pour l'immobilisation de ligands sur des supports solides et dispositif et ses procedes d'utilisation

Publications (1)

Publication Number Publication Date
CA2382121A1 true CA2382121A1 (fr) 2001-03-08

Family

ID=26848471

Family Applications (1)

Application Number Title Priority Date Filing Date
CA002382121A Abandoned CA2382121A1 (fr) 1999-08-27 2000-08-28 Procedes pour l'immobilisation de ligands sur des supports solides et dispositif et ses procedes d'utilisation

Country Status (7)

Country Link
US (3) US6492118B1 (fr)
EP (1) EP1208238B1 (fr)
JP (1) JP2003508763A (fr)
AT (1) ATE414171T1 (fr)
CA (1) CA2382121A1 (fr)
DE (1) DE60040794D1 (fr)
WO (1) WO2001016372A1 (fr)

Families Citing this family (84)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US6673908B1 (en) * 1999-02-22 2004-01-06 Nuvelo, Inc. Tumor necrosis factor receptor 2
US20020012971A1 (en) * 2000-03-20 2002-01-31 Mehta Tammy Burd PCR compatible nucleic acid sieving medium
WO2002027026A2 (fr) * 2000-09-28 2002-04-04 Matrix Technologies Corporation Support ameliore pour dosages par hybridation en phase solide
CN100357450C (zh) * 2000-10-23 2007-12-26 因哲尼控股公司 检测样品中核酸片段突变的方法和装置
EP1245272A1 (fr) * 2001-03-30 2002-10-02 Ucb S.A. Procédé de préparation de substrats ainsi que leur utilisation
US7297311B2 (en) * 2001-06-29 2007-11-20 Sysmex Corporation Automatic smear preparing apparatus and automatic sample analysis system having the same
CA2656209C (fr) * 2001-07-11 2012-11-27 Baylor College Of Medicine Procedes et dispositifs bases sur une nouvelle forme de double helice d'acide nucleique sur une surface
US10272409B2 (en) 2001-07-11 2019-04-30 Michael E. Hogan Methods and devices based upon a novel form of nucleic acid duplex on a surface
ES2262601T3 (es) * 2001-07-16 2006-12-01 Dr. Chip Biotechnology Inc. Inmobilizacion de biomoleculas.
US6995248B2 (en) 2001-07-16 2006-02-07 Dr. Chip Biotechnology, Inc. Immobilization of nucleic acids
RU2206575C2 (ru) * 2001-07-25 2003-06-20 Институт молекулярной биологии им. В.А. Энгельгардта РАН Композиция для иммобилизации биологических макромолекул в гидрогелях, способ приготовления композиции, биочип, способ проведения пцр на биочипе
US7341552B2 (en) * 2002-03-15 2008-03-11 The University Of Texas System Board Of Regents Gene sets for glioma classification
US7371852B2 (en) * 2003-01-22 2008-05-13 Serenex, Inc. Alkyl-linked nucleotide compositions
SE0300624D0 (sv) 2003-03-05 2003-03-05 Amersham Biosciences Ab A method of preparing affinity ligands
US20050112786A1 (en) * 2003-11-25 2005-05-26 Qing Wang Method of immobilizing a substance of interest to a solid phase
TW200521433A (en) * 2003-12-22 2005-07-01 We Gene Technologies Inc Cell detection chip and fabricating method thereof and method of detecting cell
US7693825B2 (en) * 2004-03-31 2010-04-06 Google Inc. Systems and methods for ranking implicit search results
GB0410448D0 (en) * 2004-05-11 2004-06-16 Hammersmith Imanet Ltd Purification methods
US7579224B2 (en) * 2005-01-21 2009-08-25 Semiconductor Energy Laboratory Co., Ltd. Method for manufacturing a thin film semiconductor device
US20070238163A1 (en) * 2006-04-05 2007-10-11 Golova Julia B Covalent attachment of biomolecules to solid supports by a polymerization method
US8241697B2 (en) 2007-12-20 2012-08-14 Abbott Point Of Care Inc. Formation of immobilized biological layers for sensing
US8268604B2 (en) * 2007-12-20 2012-09-18 Abbott Point Of Care Inc. Compositions for forming immobilized biological layers for sensing
JP2009156767A (ja) * 2007-12-27 2009-07-16 National Institute Of Advanced Industrial & Technology タンパク質アレイ基板
RU2451010C1 (ru) * 2011-01-11 2012-05-20 Закрытое Акционерное Общество "Ива Фарм" Палладиево-медные катализаторы гомогенного селективного окисления тиольных групп, комбинация и композиция на их основе и способ терапевтического воздействия
US11591637B2 (en) 2012-08-14 2023-02-28 10X Genomics, Inc. Compositions and methods for sample processing
US20140155295A1 (en) 2012-08-14 2014-06-05 10X Technologies, Inc. Capsule array devices and methods of use
US10221442B2 (en) 2012-08-14 2019-03-05 10X Genomics, Inc. Compositions and methods for sample processing
US20150005199A1 (en) * 2012-08-14 2015-01-01 10X Technologies, Inc. Compositions and methods for sample processing
US9701998B2 (en) 2012-12-14 2017-07-11 10X Genomics, Inc. Methods and systems for processing polynucleotides
US10400280B2 (en) 2012-08-14 2019-09-03 10X Genomics, Inc. Methods and systems for processing polynucleotides
US10273541B2 (en) 2012-08-14 2019-04-30 10X Genomics, Inc. Methods and systems for processing polynucleotides
US10752949B2 (en) 2012-08-14 2020-08-25 10X Genomics, Inc. Methods and systems for processing polynucleotides
US9951386B2 (en) 2014-06-26 2018-04-24 10X Genomics, Inc. Methods and systems for processing polynucleotides
US10323279B2 (en) 2012-08-14 2019-06-18 10X Genomics, Inc. Methods and systems for processing polynucleotides
US20140378349A1 (en) * 2012-08-14 2014-12-25 10X Technologies, Inc. Compositions and methods for sample processing
US10533221B2 (en) 2012-12-14 2020-01-14 10X Genomics, Inc. Methods and systems for processing polynucleotides
EP3567116A1 (fr) 2012-12-14 2019-11-13 10X Genomics, Inc. Procédés et systèmes de traitement de polynucléotides
WO2014124338A1 (fr) 2013-02-08 2014-08-14 10X Technologies, Inc. Génération de codes à barres de polynucléotides
WO2014138028A1 (fr) * 2013-03-04 2014-09-12 The Scripps Research Institute Plate-forme de réseau liquide pour l'analyse en multiplex d'interactions molécule-protéine
ES2537097B1 (es) * 2013-10-30 2016-04-05 Universitat Politècnica De València Metodo de activación química superficial de un soporte sólido en base silicio mediante anclaje covalente directo de al menos una biomolécula de ácido nucleico.
US9824068B2 (en) 2013-12-16 2017-11-21 10X Genomics, Inc. Methods and apparatus for sorting data
WO2015157567A1 (fr) 2014-04-10 2015-10-15 10X Genomics, Inc. Dispositifs fluidiques, systèmes et procédés permettant d'encapsuler et de séparer des réactifs, et leurs applications
MX2016016902A (es) 2014-06-26 2017-03-27 10X Genomics Inc Metodos para analizar acidos nucleicos de celulas individuales o poblaciones de celulas.
CN114807307A (zh) 2014-10-29 2022-07-29 10X 基因组学有限公司 用于靶核酸测序的方法和组合物
US9975122B2 (en) 2014-11-05 2018-05-22 10X Genomics, Inc. Instrument systems for integrated sample processing
CN107427808B (zh) 2015-01-12 2020-10-23 10X基因组学有限公司 用于制备核酸测序文库的方法和***以及用其制备的文库
US10697000B2 (en) 2015-02-24 2020-06-30 10X Genomics, Inc. Partition processing methods and systems
WO2016138148A1 (fr) 2015-02-24 2016-09-01 10X Genomics, Inc. Procédés pour la couverture ciblée de séquences d'acides nucléiques
US11371094B2 (en) 2015-11-19 2022-06-28 10X Genomics, Inc. Systems and methods for nucleic acid processing using degenerate nucleotides
ES2926495T3 (es) 2015-12-04 2022-10-26 10X Genomics Inc Métodos y composiciones para el análisis de ácidos nucleicos
WO2017138984A1 (fr) 2016-02-11 2017-08-17 10X Genomics, Inc. Systèmes, procédés, et milieux destinés à l'assemblage de novo de données de séquence du génome entier
WO2017197338A1 (fr) 2016-05-13 2017-11-16 10X Genomics, Inc. Systèmes microfluidiques et procédés d'utilisation
US10969394B2 (en) 2016-11-28 2021-04-06 Dana-Farber Cancer Institute, Inc. Reagents and methods for analysis of proteins and metabolites targeted by covalent probes
US10815525B2 (en) 2016-12-22 2020-10-27 10X Genomics, Inc. Methods and systems for processing polynucleotides
US10550429B2 (en) 2016-12-22 2020-02-04 10X Genomics, Inc. Methods and systems for processing polynucleotides
US10011872B1 (en) 2016-12-22 2018-07-03 10X Genomics, Inc. Methods and systems for processing polynucleotides
CN117512066A (zh) 2017-01-30 2024-02-06 10X基因组学有限公司 用于基于微滴的单细胞条形编码的方法和***
US10995333B2 (en) 2017-02-06 2021-05-04 10X Genomics, Inc. Systems and methods for nucleic acid preparation
CN109526228B (zh) 2017-05-26 2022-11-25 10X基因组学有限公司 转座酶可接近性染色质的单细胞分析
US10400235B2 (en) 2017-05-26 2019-09-03 10X Genomics, Inc. Single cell analysis of transposase accessible chromatin
US10837047B2 (en) 2017-10-04 2020-11-17 10X Genomics, Inc. Compositions, methods, and systems for bead formation using improved polymers
US10590244B2 (en) 2017-10-04 2020-03-17 10X Genomics, Inc. Compositions, methods, and systems for bead formation using improved polymers
WO2019084043A1 (fr) 2017-10-26 2019-05-02 10X Genomics, Inc. Méthodes et systèmes de préparation d'acide nucléique et d'analyse de chromatine
EP4241882A3 (fr) 2017-10-27 2023-12-06 10X Genomics, Inc. Procédés de préparation et d'analyse d'échantillons
WO2019099751A1 (fr) 2017-11-15 2019-05-23 10X Genomics, Inc. Perles de gel fonctionnalisées
US10829815B2 (en) 2017-11-17 2020-11-10 10X Genomics, Inc. Methods and systems for associating physical and genetic properties of biological particles
WO2019108851A1 (fr) 2017-11-30 2019-06-06 10X Genomics, Inc. Systèmes et procédés de préparation et d'analyse d'acides nucléiques
EP3752832A1 (fr) 2018-02-12 2020-12-23 10X Genomics, Inc. Procédés de caractérisation d'analytes multiples à partir de cellules individuelles ou de populations cellulaires
US11639928B2 (en) 2018-02-22 2023-05-02 10X Genomics, Inc. Methods and systems for characterizing analytes from individual cells or cell populations
EP3775271A1 (fr) 2018-04-06 2021-02-17 10X Genomics, Inc. Systèmes et procédés de contrôle de qualité dans un traitement de cellules uniques
US11932899B2 (en) 2018-06-07 2024-03-19 10X Genomics, Inc. Methods and systems for characterizing nucleic acid molecules
US11703427B2 (en) 2018-06-25 2023-07-18 10X Genomics, Inc. Methods and systems for cell and bead processing
US20200032335A1 (en) 2018-07-27 2020-01-30 10X Genomics, Inc. Systems and methods for metabolome analysis
US11459607B1 (en) 2018-12-10 2022-10-04 10X Genomics, Inc. Systems and methods for processing-nucleic acid molecules from a single cell using sequential co-partitioning and composite barcodes
US11845983B1 (en) 2019-01-09 2023-12-19 10X Genomics, Inc. Methods and systems for multiplexing of droplet based assays
WO2020168013A1 (fr) 2019-02-12 2020-08-20 10X Genomics, Inc. Procédés de traitement de molécules d'acides nucléiques
US11851683B1 (en) 2019-02-12 2023-12-26 10X Genomics, Inc. Methods and systems for selective analysis of cellular samples
US11467153B2 (en) 2019-02-12 2022-10-11 10X Genomics, Inc. Methods for processing nucleic acid molecules
US11655499B1 (en) 2019-02-25 2023-05-23 10X Genomics, Inc. Detection of sequence elements in nucleic acid molecules
US11920183B2 (en) 2019-03-11 2024-03-05 10X Genomics, Inc. Systems and methods for processing optically tagged beads
CA3135206A1 (fr) * 2019-04-29 2020-11-05 Pierre Indermuhle Procedes et systemes de detection de molecule unique integree sur puce
WO2021153717A1 (fr) * 2020-01-31 2021-08-05 池田　壽文 Chromatographie d'affinité à base d'acide nucléique artificiel
US11851700B1 (en) 2020-05-13 2023-12-26 10X Genomics, Inc. Methods, kits, and compositions for processing extracellular molecules
EP4298244A1 (fr) 2021-02-23 2024-01-03 10X Genomics, Inc. Analyse à base de sonde d'acides nucléiques et de protéines

Family Cites Families (20)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JPS5927900A (ja) 1982-08-09 1984-02-14 Wakunaga Seiyaku Kk 固定化オリゴヌクレオチド
US4683202A (en) 1985-03-28 1987-07-28 Cetus Corporation Process for amplifying nucleic acid sequences
US5034428A (en) 1986-06-19 1991-07-23 Board Of Regents Of The University Of Washington Immobilized biomolecules and method of making same
US4898824A (en) 1986-12-09 1990-02-06 Miles Inc. Crosslinked polyacrylamide-sulfhydryl polymer for immobilization of biologically active substances
US5237016A (en) 1989-01-05 1993-08-17 Siska Diagnostics, Inc. End-attachment of oligonucleotides to polyacrylamide solid supports for capture and detection of nucleic acids
US6040138A (en) 1995-09-15 2000-03-21 Affymetrix, Inc. Expression monitoring by hybridization to high density oligonucleotide arrays
US5324650A (en) * 1990-03-20 1994-06-28 E. I. Du Pont De Nemours And Company Situ process for production of conjugates
US5176966A (en) 1990-11-19 1993-01-05 Ballard Power Systems Inc. Fuel cell membrane electrode and seal assembly
US5482836A (en) 1993-01-14 1996-01-09 The Regents Of The University Of California DNA purification by triplex-affinity capture and affinity capture electrophoresis
US5807522A (en) 1994-06-17 1998-09-15 The Board Of Trustees Of The Leland Stanford Junior University Methods for fabricating microarrays of biological samples
KR100399813B1 (ko) * 1994-12-14 2004-06-09 가부시키가이샤 니콘 노광장치
US5599695A (en) 1995-02-27 1997-02-04 Affymetrix, Inc. Printing molecular library arrays using deprotection agents solely in the vapor phase
US6013440A (en) 1996-03-11 2000-01-11 Affymetrix, Inc. Nucleic acid affinity columns
US5728296A (en) * 1996-03-20 1998-03-17 Bio-Rad Laboratories, Inc. Selective recognition of solutes in chromatographic media by artificially created affinity
US6083763A (en) 1996-12-31 2000-07-04 Genometrix Inc. Multiplexed molecular analysis apparatus and method
US5837860A (en) 1997-03-05 1998-11-17 Molecular Tool, Inc. Covalent attachment of nucleic acid molecules onto solid-phases via disulfide bonds
US5932711A (en) 1997-03-05 1999-08-03 Mosaic Technologies, Inc. Nucleic acid-containing polymerizable complex
US5858653A (en) 1997-09-30 1999-01-12 Surmodics, Inc. Reagent and method for attaching target molecules to a surface
US6268131B1 (en) * 1997-12-15 2001-07-31 Sequenom, Inc. Mass spectrometric methods for sequencing nucleic acids
US6093370A (en) 1998-06-11 2000-07-25 Hitachi, Ltd. Polynucleotide separation method and apparatus therefor

Also Published As

Publication number Publication date
WO2001016372A1 (fr) 2001-03-08
WO2001016372A9 (fr) 2002-09-12
DE60040794D1 (de) 2008-12-24
EP1208238A1 (fr) 2002-05-29
JP2003508763A (ja) 2003-03-04
US20030143570A1 (en) 2003-07-31
US6492118B1 (en) 2002-12-10
EP1208238B1 (fr) 2008-11-12
ATE414171T1 (de) 2008-11-15
US20030143569A1 (en) 2003-07-31

Similar Documents

Publication Publication Date Title
US6492118B1 (en) Methods of immobilizing ligands on solid supports
Kandow et al. Polyacrylamide hydrogels for cell mechanics: steps toward optimization and alternative uses
US6180770B1 (en) Nucleic acid-containing polymerizable complex
EP1208126B1 (fr) Brosses polymeres destinees a immobiliser des molecules sur une surface ou un substrat, dans lesquelles les polymeres presentent des segments dispersables ou solubles dans l'eau et capteurs y etant lies
US6692912B1 (en) Nucleic acid-containing polymerizable complex
US9834617B2 (en) Method for immobilizing biologic molecules on solid surfaces
JP5588430B2 (ja) 標識独立検出のための表面及びその方法
MXPA01007638A (es) Configuracion replicable de sonda.
US20020037509A1 (en) Polymer coated surfaces for microarray applications
US7785770B2 (en) Sugar-containing hydrogel for immobilization
KR20140137366A (ko) 분석 적용용 고분자 스카폴드
AU2004265263A1 (en) Colorable microspheres for DNA and protein microarray
KR101319104B1 (ko) 수퍼코일드 ｄｎａ를 고정하기 위한 방법 및 ｄｎａ 수선을분석하기 위한 이의 용도
AU780882B2 (en) Methods of immobilizing ligands on solid supports and apparatus and methods of use therefor
JP2003329685A (ja) プローブ分子が固定された検出具の処理方法及び水性処理液
US20030003480A1 (en) Reactive solid support and DNA fragment detection tool
Peris et al. Photopolymerization as a promising method to sense biorecognition events
RU2004134192A (ru) Нанодиагностическая тест-система для выявления вируса гепатитов
JP4397006B2 (ja) 生体関連物質固定化ゲルの製造方法
JP2001136972A (ja) 核酸固定化高分子ゲル及びその製造方法
JP2003194815A (ja) 反応性固相担体及びｄｎａ断片検出用具
Zhang Differential adhesion of microspheres to substrate* surfaces mediated by DNA hybridization
JP2003028871A (ja) 反応性固相担体及びｄｎａ断片検出用具
JP2002365292A (ja) 反応性固相担体及びｄｎａ断片検出用具

Legal Events

Date Code Title Description
FZDE Discontinued