CA2362979A1 - Method for isolating nucleic acids - Google Patents

Method for isolating nucleic acids Download PDF

Info

Publication number
CA2362979A1
CA2362979A1 CA002362979A CA2362979A CA2362979A1 CA 2362979 A1 CA2362979 A1 CA 2362979A1 CA 002362979 A CA002362979 A CA 002362979A CA 2362979 A CA2362979 A CA 2362979A CA 2362979 A1 CA2362979 A1 CA 2362979A1
Authority
CA
Canada
Prior art keywords
weight
water
insoluble
crosslinker
monomer
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Abandoned
Application number
CA002362979A
Other languages
English (en)
French (fr)
Inventor
Wolfgang Podszun
Rainer Neumann
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Bayer AG
Original Assignee
Individual
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Individual filed Critical Individual
Publication of CA2362979A1 publication Critical patent/CA2362979A1/en
Abandoned legal-status Critical Current

Links

Classifications

    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09Recombinant DNA-technology
    • C12N15/10Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/1003Extracting or separating nucleic acids from biological samples, e.g. pure separation or isolation methods; Conditions, buffers or apparatuses therefor
    • C12N15/1006Extracting or separating nucleic acids from biological samples, e.g. pure separation or isolation methods; Conditions, buffers or apparatuses therefor by means of a solid support carrier, e.g. particles, polymers
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12QMEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6806Preparing nucleic acids for analysis, e.g. for polymerase chain reaction [PCR] assay

Landscapes

  • Chemical & Material Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Organic Chemistry (AREA)
  • Wood Science & Technology (AREA)
  • Genetics & Genomics (AREA)
  • Zoology (AREA)
  • Analytical Chemistry (AREA)
  • Biomedical Technology (AREA)
  • Biotechnology (AREA)
  • General Engineering & Computer Science (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Microbiology (AREA)
  • Physics & Mathematics (AREA)
  • Molecular Biology (AREA)
  • Biochemistry (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Biophysics (AREA)
  • General Health & Medical Sciences (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Immunology (AREA)
  • Crystallography & Structural Chemistry (AREA)
  • Plant Pathology (AREA)
  • Addition Polymer Or Copolymer, Post-Treatments, Or Chemical Modifications (AREA)
  • Saccharide Compounds (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
CA002362979A 1999-02-19 2000-02-09 Method for isolating nucleic acids Abandoned CA2362979A1 (en)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
DE19907023.7 1999-02-19
DE19907023A DE19907023A1 (de) 1999-02-19 1999-02-19 Verfahren zur Isolierung von Nucleinsäuren
PCT/EP2000/001028 WO2000049031A2 (de) 1999-02-19 2000-02-09 Verfahren zur isolierung von nucleinsäuren

Publications (1)

Publication Number Publication Date
CA2362979A1 true CA2362979A1 (en) 2000-08-24

Family

ID=7898058

Family Applications (1)

Application Number Title Priority Date Filing Date
CA002362979A Abandoned CA2362979A1 (en) 1999-02-19 2000-02-09 Method for isolating nucleic acids

Country Status (6)

Country Link
EP (1) EP1155026A2 (de)
JP (1) JP2002537306A (de)
AU (1) AU2547100A (de)
CA (1) CA2362979A1 (de)
DE (1) DE19907023A1 (de)
WO (1) WO2000049031A2 (de)

Cited By (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US9663779B2 (en) 2008-12-23 2017-05-30 Qiagen Gmbh Nucleic acid purification method
US11001703B2 (en) 2015-12-25 2021-05-11 Kuraray Co., Ltd. Aqueous emulsion and adhesive using same

Families Citing this family (9)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
GB9425138D0 (en) 1994-12-12 1995-02-08 Dynal As Isolation of nucleic acid
US6914137B2 (en) 1997-12-06 2005-07-05 Dna Research Innovations Limited Isolation of nucleic acids
US6958392B2 (en) * 1998-10-09 2005-10-25 Whatman, Inc. Methods for the isolation of nucleic acids and for quantitative DNA extraction and detection for leukocyte evaluation in blood products
US6383783B1 (en) * 1999-09-21 2002-05-07 3M Innovative Properties Company Nucleic acid isolation by adhering to hydrophobic solid phase and removing with nonionic surfactant
DE10261910A1 (de) * 2002-12-30 2004-07-15 Polymerics Gmbh Adsorbermaterial für Blut-, Blutplasma- und Albuminreinigungsverfahren
US7098253B2 (en) 2004-05-20 2006-08-29 3M Innovative Properties Company Macroporous ion exchange resins
US7674835B2 (en) 2005-12-21 2010-03-09 3M Innovative Properties Company Method of making macroporous anion exchange resins
US7683100B2 (en) 2005-12-21 2010-03-23 3M Innovative Properties Company Method of making macroporous cation exchange resins
US7674836B2 (en) 2006-07-28 2010-03-09 3M Innovative Properties Company Method of making macroporous cation exchange resins

Family Cites Families (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP0214909A1 (de) * 1985-09-02 1987-03-18 Plant Genetic Systems N.V. Mittel und Verfahren zur Übertragung von Proteinen und/oder Nukleinsäuren auf eine unterstützte Rezeptoroberfläche
DE3935098C2 (de) * 1989-10-21 1995-05-24 Macherey Nagel & Co Chem Chromatographisches Trägermaterial sowie seine Verwendung in einem Verfahren zur chromatographischen Trennung von Nukleinsäuren
NZ247458A (en) * 1992-05-29 1994-09-27 Rohm & Haas Crosslinked beads predominantly formed from methacrylic anhydride, preparation and use in separation processes
US5804684A (en) * 1995-08-24 1998-09-08 The Theobald Smith Research Institute, Inc. Method for isolating nucleic acids
JP4693944B2 (ja) * 1996-03-20 2011-06-01 ベーイーオー・メリュー 核酸単離法

Cited By (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US9663779B2 (en) 2008-12-23 2017-05-30 Qiagen Gmbh Nucleic acid purification method
US11001703B2 (en) 2015-12-25 2021-05-11 Kuraray Co., Ltd. Aqueous emulsion and adhesive using same

Also Published As

Publication number Publication date
AU2547100A (en) 2000-09-04
EP1155026A2 (de) 2001-11-21
WO2000049031A3 (de) 2001-04-12
WO2000049031A2 (de) 2000-08-24
DE19907023A1 (de) 2000-08-24
JP2002537306A (ja) 2002-11-05

Similar Documents

Publication Publication Date Title
US6590094B2 (en) Superparamagnetic bead polymers
US10221411B2 (en) Process for manufacturing a composite sorbent material for chromatographical separation of biopolymers
US8598338B2 (en) Compositions and methods for using a solid support to purify DNA
JP4377550B2 (ja) pH依存型イオン交換マトリックス及び核酸の単離におけるその使用法
EP1179056B1 (de) Mischbett-festphase und dessen verwendung zur isolation von nukleinsäuren
JP4693944B2 (ja) 核酸単離法
CA2158485C (en) Methods for capture and selective release of nucleic acids using weakly basic polymer and amplification of same
JP3395906B2 (ja) 核酸断片の分離法
JP3397737B2 (ja) 核酸の抽出方法
CA2362979A1 (en) Method for isolating nucleic acids
US6503762B1 (en) Magnetic carrier, preparation thereof, and method of extraction of nucleic acid
JP2009118858A (ja) 常磁性粒子を用いた集細胞及びライセート清澄化
US8778842B2 (en) Sample lysis and coating of reaction surface
EP0095932A1 (de) Die Verwendung eines teilchenförmigen Polymers als Träger für biologische Substanzen und dergleichen und solche auf dem Träger gebundene Substanzen
JP3689897B2 (ja) 核酸吸着剤
JP2005083905A (ja) 磁性粒子
JP3689896B2 (ja) 核酸吸着剤
JP2005083904A (ja) 磁性粒子
JP4161167B2 (ja) ウイルス濃縮方法、ウイルス検出方法およびウイルス濃縮用試薬
JP2009118773A (ja) 核酸分析法
CA2552010A1 (en) Method for enriching and stabilising components which contain dna and which are made of biological materials
CN117861627A (zh) 钾离子微球、质粒提取试剂和应用

Legal Events

Date Code Title Description
EEER Examination request
FZDE Dead