CA2362977A1 - Method of detecting shikimic acid - Google Patents
Method of detecting shikimic acid Download PDFInfo
- Publication number
- CA2362977A1 CA2362977A1 CA002362977A CA2362977A CA2362977A1 CA 2362977 A1 CA2362977 A1 CA 2362977A1 CA 002362977 A CA002362977 A CA 002362977A CA 2362977 A CA2362977 A CA 2362977A CA 2362977 A1 CA2362977 A1 CA 2362977A1
- Authority
- CA
- Canada
- Prior art keywords
- chromophore
- shikimic acid
- detecting
- acid
- tissue extracts
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Granted
Links
- JXOHGGNKMLTUBP-HSUXUTPPSA-N shikimic acid Chemical compound O[C@@H]1CC(C(O)=O)=C[C@@H](O)[C@H]1O JXOHGGNKMLTUBP-HSUXUTPPSA-N 0.000 title abstract 3
- JXOHGGNKMLTUBP-JKUQZMGJSA-N shikimic acid Natural products O[C@@H]1CC(C(O)=O)=C[C@H](O)[C@@H]1O JXOHGGNKMLTUBP-JKUQZMGJSA-N 0.000 title abstract 3
- KHIWWQKSHDUIBK-UHFFFAOYSA-N periodic acid Chemical compound OI(=O)(=O)=O KHIWWQKSHDUIBK-UHFFFAOYSA-N 0.000 abstract 3
- 239000000284 extract Substances 0.000 abstract 2
- LSNNMFCWUKXFEE-UHFFFAOYSA-N Sulfurous acid Chemical compound OS(O)=O LSNNMFCWUKXFEE-UHFFFAOYSA-N 0.000 abstract 1
- 239000003153 chemical reaction reagent Substances 0.000 abstract 1
- 230000001590 oxidative effect Effects 0.000 abstract 1
- 230000000087 stabilizing effect Effects 0.000 abstract 1
Classifications
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
- G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
- G01N33/66—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving blood sugars, e.g. galactose
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
- G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
- G01N33/52—Use of compounds or compositions for colorimetric, spectrophotometric or fluorometric investigation, e.g. use of reagent paper and including single- and multilayer analytical elements
Landscapes
- Health & Medical Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Hematology (AREA)
- Engineering & Computer Science (AREA)
- Immunology (AREA)
- Urology & Nephrology (AREA)
- Molecular Biology (AREA)
- Chemical & Material Sciences (AREA)
- Biomedical Technology (AREA)
- Physics & Mathematics (AREA)
- Microbiology (AREA)
- Cell Biology (AREA)
- Food Science & Technology (AREA)
- Medicinal Chemistry (AREA)
- Biotechnology (AREA)
- Analytical Chemistry (AREA)
- Biochemistry (AREA)
- General Health & Medical Sciences (AREA)
- General Physics & Mathematics (AREA)
- Pathology (AREA)
- Diabetes (AREA)
- Investigating Or Analyzing Non-Biological Materials By The Use Of Chemical Means (AREA)
Abstract
The invention provides methods of detecting shikimic acid comprising the steps of: oxidizing shikimic acid with periodic acid or a reagent comprising periodate and periodic acid; adding a strong base to generate a chromophore; stabilizing the chromophore with sulfite;
detecting the presence of the chromophore; and optionally quantifying the amount of the chromophore. The methods of the invention can be performed directly with tissue extracts, preferably plant tissue extracts.
detecting the presence of the chromophore; and optionally quantifying the amount of the chromophore. The methods of the invention can be performed directly with tissue extracts, preferably plant tissue extracts.
Applications Claiming Priority (3)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
US25328299A | 1999-02-19 | 1999-02-19 | |
US09/253,282 | 1999-02-19 | ||
PCT/GB2000/000358 WO2000049399A1 (en) | 1999-02-19 | 2000-02-07 | Method of detecting shikimic acid |
Publications (2)
Publication Number | Publication Date |
---|---|
CA2362977A1 true CA2362977A1 (en) | 2000-08-24 |
CA2362977C CA2362977C (en) | 2008-08-12 |
Family
ID=22959622
Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
CA002362977A Expired - Fee Related CA2362977C (en) | 1999-02-19 | 2000-02-07 | Method of detecting shikimic acid |
Country Status (11)
Country | Link |
---|---|
JP (1) | JP4410946B2 (en) |
CN (1) | CN1164943C (en) |
AR (1) | AR022590A1 (en) |
AU (1) | AU774136B2 (en) |
BR (1) | BR0008953B1 (en) |
CA (1) | CA2362977C (en) |
IL (2) | IL144946A0 (en) |
MX (1) | MXPA01008358A (en) |
TW (1) | TWI223067B (en) |
WO (1) | WO2000049399A1 (en) |
ZA (1) | ZA200106824B (en) |
Families Citing this family (3)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
CN102980866A (en) * | 2012-11-26 | 2013-03-20 | 东北农业大学 | Kit for testing in-vivo shikimic acid content of plants |
CN107084943B (en) * | 2017-04-28 | 2019-06-25 | 浙江大学 | A kind of method of quick obtaining transgenic corns glyphosate tolerant phenotype |
CN106896077B (en) * | 2017-04-28 | 2019-05-28 | 浙江大学 | The detection method of transgenic corns glyphosate tolerant phenotype based on imaging-PAM |
Family Cites Families (2)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
US4294911A (en) * | 1979-06-18 | 1981-10-13 | Eastman Kodak Company | Development of light-sensitive quinone diazide compositions using sulfite stabilizer |
DK100592D0 (en) * | 1992-08-10 | 1992-08-10 | Mouritsen & Elsner Aps | METHOD OF CHEMICAL CONNECTION ON SOLID PHASES |
-
2000
- 2000-02-03 TW TW089102005A patent/TWI223067B/en not_active IP Right Cessation
- 2000-02-07 AU AU23098/00A patent/AU774136B2/en not_active Ceased
- 2000-02-07 BR BRPI0008953-2A patent/BR0008953B1/en not_active IP Right Cessation
- 2000-02-07 CN CNB008064288A patent/CN1164943C/en not_active Expired - Fee Related
- 2000-02-07 MX MXPA01008358A patent/MXPA01008358A/en not_active IP Right Cessation
- 2000-02-07 IL IL14494600A patent/IL144946A0/en active IP Right Grant
- 2000-02-07 JP JP2000600090A patent/JP4410946B2/en not_active Expired - Fee Related
- 2000-02-07 WO PCT/GB2000/000358 patent/WO2000049399A1/en active IP Right Grant
- 2000-02-07 CA CA002362977A patent/CA2362977C/en not_active Expired - Fee Related
- 2000-02-14 AR ARP000100628A patent/AR022590A1/en active IP Right Grant
-
2001
- 2001-08-16 IL IL144946A patent/IL144946A/en not_active IP Right Cessation
- 2001-08-17 ZA ZA200106824A patent/ZA200106824B/en unknown
Also Published As
Publication number | Publication date |
---|---|
CA2362977C (en) | 2008-08-12 |
ZA200106824B (en) | 2003-02-17 |
AU2309800A (en) | 2000-09-04 |
CN1351711A (en) | 2002-05-29 |
CN1164943C (en) | 2004-09-01 |
JP2002537559A (en) | 2002-11-05 |
MXPA01008358A (en) | 2003-06-06 |
AR022590A1 (en) | 2002-09-04 |
IL144946A0 (en) | 2002-06-30 |
TWI223067B (en) | 2004-11-01 |
IL144946A (en) | 2007-03-08 |
JP4410946B2 (en) | 2010-02-10 |
WO2000049399A1 (en) | 2000-08-24 |
AU774136B2 (en) | 2004-06-17 |
BR0008953A (en) | 2001-11-27 |
BR0008953B1 (en) | 2012-09-18 |
Similar Documents
Publication | Publication Date | Title |
---|---|---|
CA2375453A1 (en) | Bidirectional lateral flow test strip and method | |
EP1115389A4 (en) | Fructosamine oxidase: antagonists and inhibitors | |
AU3320897A (en) | Analyte concentration measurement using a hollow frustum | |
ID30385A (en) | COMPOSITION FOR LOSS OF INFECTION BASED ON H202, ACID AND METAL IONS | |
DE69928618D1 (en) | Acrylic acid-maleic acid copolymer (or its salts), method of preparation and use, and process for the preparation of an aqueous solution of a salt of maleic acid of low impurity content | |
NO984355D0 (en) | Procedure for measuring an analyte's concentration | |
TR199802441A3 (en) | Method and composition for the treatment and prevention of the presence of excess uric acid in the blood. | |
DE60026844D1 (en) | PROCESS FOR THE PREPARATION OF 3,3-DIMETHYLBUTANAL | |
AU6194998A (en) | Aqueous cosmetic composition containing stably solubilized uric acid and water-soluble polymer and method for stably solubilizing uric acid in aqueous cosmetic composition | |
AU6364699A (en) | Detection of analytes | |
AU5422099A (en) | Method for producing acrolein and/or acrylic acid from propane | |
EP0667524A3 (en) | Electrochemical determination of the oxygen concentration. | |
CA2416062A1 (en) | Resolution of the enantiomers of amlodipine | |
AUPP713498A0 (en) | A method of detecting blood | |
DK0946812T3 (en) | Method for improved shrink resistance | |
AU5512599A (en) | Method for producing acrolein and/or acrylic acid from propane | |
AU5512699A (en) | Method for producing acrolein and/or acrylic acid from propane | |
CA2362977A1 (en) | Method of detecting shikimic acid | |
AU3825297A (en) | Determination of aqueous fluid surfactant concentration | |
AU4439999A (en) | Metal yo-yo and method for manufacture | |
AU3460800A (en) | Method of bleaching blood samples, diagnostic method and diagnostic kit using the same | |
BR9705520B1 (en) | Process for the preparation of aliphatic diacids from Wash waters from a cyclohexane oxidation process and containing peroxides. | |
EP1083890A4 (en) | N-acetyl 3-chloroprocainamide, acid addition salts thereof, and methods of use | |
CA2380227A1 (en) | Improved liquid smoke coloring agent solution made from liquid smoke organic precipitate | |
AU2002240829A1 (en) | Method for determining concentrations of analytes |
Legal Events
Date | Code | Title | Description |
---|---|---|---|
EEER | Examination request | ||
MKLA | Lapsed |
Effective date: 20150209 |