CA2362977A1 - Method of detecting shikimic acid - Google Patents

Method of detecting shikimic acid Download PDF

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Publication number
CA2362977A1
CA2362977A1 CA002362977A CA2362977A CA2362977A1 CA 2362977 A1 CA2362977 A1 CA 2362977A1 CA 002362977 A CA002362977 A CA 002362977A CA 2362977 A CA2362977 A CA 2362977A CA 2362977 A1 CA2362977 A1 CA 2362977A1
Authority
CA
Canada
Prior art keywords
chromophore
shikimic acid
detecting
acid
tissue extracts
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Granted
Application number
CA002362977A
Other languages
French (fr)
Other versions
CA2362977C (en
Inventor
Thomas Houston Cromartie
Nicholas David Polge
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Syngenta Ltd
Original Assignee
Individual
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Individual filed Critical Individual
Publication of CA2362977A1 publication Critical patent/CA2362977A1/en
Application granted granted Critical
Publication of CA2362977C publication Critical patent/CA2362977C/en
Anticipated expiration legal-status Critical
Expired - Fee Related legal-status Critical Current

Links

Classifications

    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/66—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving blood sugars, e.g. galactose
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/52—Use of compounds or compositions for colorimetric, spectrophotometric or fluorometric investigation, e.g. use of reagent paper and including single- and multilayer analytical elements

Landscapes

  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Hematology (AREA)
  • Engineering & Computer Science (AREA)
  • Immunology (AREA)
  • Urology & Nephrology (AREA)
  • Molecular Biology (AREA)
  • Chemical & Material Sciences (AREA)
  • Biomedical Technology (AREA)
  • Physics & Mathematics (AREA)
  • Microbiology (AREA)
  • Cell Biology (AREA)
  • Food Science & Technology (AREA)
  • Medicinal Chemistry (AREA)
  • Biotechnology (AREA)
  • Analytical Chemistry (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • General Physics & Mathematics (AREA)
  • Pathology (AREA)
  • Diabetes (AREA)
  • Investigating Or Analyzing Non-Biological Materials By The Use Of Chemical Means (AREA)

Abstract

The invention provides methods of detecting shikimic acid comprising the steps of: oxidizing shikimic acid with periodic acid or a reagent comprising periodate and periodic acid; adding a strong base to generate a chromophore; stabilizing the chromophore with sulfite;
detecting the presence of the chromophore; and optionally quantifying the amount of the chromophore. The methods of the invention can be performed directly with tissue extracts, preferably plant tissue extracts.
CA002362977A 1999-02-19 2000-02-07 Method of detecting shikimic acid Expired - Fee Related CA2362977C (en)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
US25328299A 1999-02-19 1999-02-19
US09/253,282 1999-02-19
PCT/GB2000/000358 WO2000049399A1 (en) 1999-02-19 2000-02-07 Method of detecting shikimic acid

Publications (2)

Publication Number Publication Date
CA2362977A1 true CA2362977A1 (en) 2000-08-24
CA2362977C CA2362977C (en) 2008-08-12

Family

ID=22959622

Family Applications (1)

Application Number Title Priority Date Filing Date
CA002362977A Expired - Fee Related CA2362977C (en) 1999-02-19 2000-02-07 Method of detecting shikimic acid

Country Status (11)

Country Link
JP (1) JP4410946B2 (en)
CN (1) CN1164943C (en)
AR (1) AR022590A1 (en)
AU (1) AU774136B2 (en)
BR (1) BR0008953B1 (en)
CA (1) CA2362977C (en)
IL (2) IL144946A0 (en)
MX (1) MXPA01008358A (en)
TW (1) TWI223067B (en)
WO (1) WO2000049399A1 (en)
ZA (1) ZA200106824B (en)

Families Citing this family (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN102980866A (en) * 2012-11-26 2013-03-20 东北农业大学 Kit for testing in-vivo shikimic acid content of plants
CN107084943B (en) * 2017-04-28 2019-06-25 浙江大学 A kind of method of quick obtaining transgenic corns glyphosate tolerant phenotype
CN106896077B (en) * 2017-04-28 2019-05-28 浙江大学 The detection method of transgenic corns glyphosate tolerant phenotype based on imaging-PAM

Family Cites Families (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4294911A (en) * 1979-06-18 1981-10-13 Eastman Kodak Company Development of light-sensitive quinone diazide compositions using sulfite stabilizer
DK100592D0 (en) * 1992-08-10 1992-08-10 Mouritsen & Elsner Aps METHOD OF CHEMICAL CONNECTION ON SOLID PHASES

Also Published As

Publication number Publication date
CA2362977C (en) 2008-08-12
ZA200106824B (en) 2003-02-17
AU2309800A (en) 2000-09-04
CN1351711A (en) 2002-05-29
CN1164943C (en) 2004-09-01
JP2002537559A (en) 2002-11-05
MXPA01008358A (en) 2003-06-06
AR022590A1 (en) 2002-09-04
IL144946A0 (en) 2002-06-30
TWI223067B (en) 2004-11-01
IL144946A (en) 2007-03-08
JP4410946B2 (en) 2010-02-10
WO2000049399A1 (en) 2000-08-24
AU774136B2 (en) 2004-06-17
BR0008953A (en) 2001-11-27
BR0008953B1 (en) 2012-09-18

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Legal Events

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EEER Examination request
MKLA Lapsed

Effective date: 20150209